AlfalfaGEDB | MsG0880047267.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047267.01

KEGG: K22849
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047267.01.T01	XP_003609890.1	96.622	148	5	0	1	148	194	341	5.21E-98	295

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047267.01.T01	Q9C5W0	53.191	94	39	2	43	136	261	349	3.21E-25	101

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048016.01	MsG0880047267.01	0.810982	8.791583e-51	5.377579e-48
MsG0080048463.01	MsG0880047267.01	0.870948	9.914934e-67	3.928891e-63
MsG0080048464.01	MsG0880047267.01	0.879085	1.661979e-69	8.891419e-66
MsG0080048683.01	MsG0880047267.01	0.827307	1.681709e-54	1.617439e-51
MsG0080048748.01	MsG0880047267.01	0.814405	1.566591e-51	1.050284e-48
MsG0080048826.01	MsG0880047267.01	0.819336	1.225305e-52	9.401197e-50
MsG0080048849.01	MsG0880047267.01	0.836962	6.878099e-57	8.806259e-54
MsG0080048865.01	MsG0880047267.01	0.813869	2.056910e-51	1.359052e-48
MsG0080048938.01	MsG0880047267.01	0.834996	2.169732e-56	2.618287e-53
MsG0080049069.01	MsG0880047267.01	0.822903	1.846753e-53	1.566319e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047267.01.T01	MTR_4g124080	96.622	148	5	0	1	148	194	341	6.30e-102	295

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047267.01.T01	AT1G48300	53.191	94	39	2	43	136	261	349	3.27e-26	101

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Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGGATTGAAGTTTGCA+TGG	0.512458	8:+83976199	MsG0880047267.01.T01:CDS
AACCCTCTCTGCATTGGAGT+TGG	0.545052	8:+83976416	MsG0880047267.01.T01:CDS
AAGAGGATTGAAGTTTGCAT+GGG	0.571804	8:+83976200	MsG0880047267.01.T01:CDS
AATAATGCATCTCTTGTTAC+TGG	0.282326	8:+83976137	MsG0880047267.01.T01:CDS
AGACCAACTCCAATGCAGAG+AGG	0.696753	8:-83976419	None:intergenic
AGAGGATGTGGATACTATTG+TGG	0.582949	8:+83976442	MsG0880047267.01.T01:CDS
AGGGGAGGATTACAAAGATG+TGG	0.525816	8:+83976475	MsG0880047267.01.T01:CDS
ATAATGCATCTCTTGTTACT+GGG	0.551376	8:+83976138	MsG0880047267.01.T01:CDS
ATGTGGATACTATTGTGGCT+AGG	0.581180	8:+83976447	MsG0880047267.01.T01:CDS
ATTGTTACAAGAGTTTGAGA+AGG	0.637353	8:+83976253	MsG0880047267.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAATGCATCTCTTGTTAC+TGG	+	Chr8:83976137-83976156	MsG0880047267.01.T01:CDS	30.0%
ATAATGCATCTCTTGTTACT+GGG	+	Chr8:83976138-83976157	MsG0880047267.01.T01:CDS	30.0%
ATTGTTACAAGAGTTTGAGA+AGG	+	Chr8:83976253-83976272	MsG0880047267.01.T01:CDS	30.0%
GAATTCTGAATTCTCACATT+AGG	-	Chr8:83976344-83976363	None:intergenic	30.0%
GAATTCTGTTGATCTTAACA+TGG	+	Chr8:83976358-83976377	MsG0880047267.01.T01:CDS	30.0%
GGTAACAAATGCAAAAAATC+AGG	+	Chr8:83976221-83976240	MsG0880047267.01.T01:CDS	30.0%
GTTTAACAAAGAGACTAATG+TGG	+	Chr8:83976160-83976179	MsG0880047267.01.T01:CDS	30.0%
TACTTCACATCATCATGTTA+TGG	+	Chr8:83976100-83976119	MsG0880047267.01.T01:CDS	30.0%
AAAGAGGATTGAAGTTTGCA+TGG	+	Chr8:83976199-83976218	MsG0880047267.01.T01:CDS	35.0%
AAGAGGATTGAAGTTTGCAT+GGG	+	Chr8:83976200-83976219	MsG0880047267.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	83976077	83976523	83976077	ID=MsG0880047267.01;Name=MsG0880047267.01
Chr8	mRNA	83976077	83976523	83976077	ID=MsG0880047267.01.T01;Parent=MsG0880047267.01;Name=MsG0880047267.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr8	exon	83976077	83976523	83976077	ID=MsG0880047267.01.T01:exon:35845;Parent=MsG0880047267.01.T01
Chr8	CDS	83976077	83976523	83976077	ID=MsG0880047267.01.T01:cds;Parent=MsG0880047267.01.T01

Gene Sequence

>MsG0880047267.01.T01

ATGCTATCCATACCTGAAGATTCTACTTCACATCATCATGTTATGGATGTGTGCACTACAAATAATGCATCTCTTGTTACTGGGTTTAACAAAGAGACTAATGTGGTTATCCCTGCAGCTCAAAAGAGGATTGAAGTTTGCATGGGTAACAAATGCAAAAAATCAGGGGCTGCTACATTGTTACAAGAGTTTGAGAAGGTAGTAGGTGTTGAAGGTGAAGGTGTTGTTGTTGGATGCAAGTGTATGGGGAAGTGTAAAACTGCTCCTAATGTGAGAATTCAGAATTCTGTTGATCTTAACATGGTTCAGGGAATTGATGATTCTGTTAAAATTCCAAGTAACCCTCTCTGCATTGGAGTTGGTCTAGAGGATGTGGATACTATTGTGGCTAGGTTTTTAGGGGAGGATTACAAAGATGTGGGTACGGTTGCTGCAACTTCTTCATGA

Protein sequence

>MsG0880047267.01.T01

MLSIPEDSTSHHHVMDVCTTNNASLVTGFNKETNVVIPAAQKRIEVCMGNKCKKSGAATLLQEFEKVVGVEGEGVVVGCKCMGKCKTAPNVRIQNSVDLNMVQGIDDSVKIPSNPLCIGVGLEDVDTIVARFLGEDYKDVGTVAATSS*