AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047267.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047267.01.T01 MTR_4g124080 96.622 148 5 0 1 148 194 341 6.30e-102 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047267.01.T01 AT1G48300 53.191 94 39 2 43 136 261 349 3.27e-26 101

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATGCATCTCTTGTTAC+TGG 0.282326 8:+83976137 MsG0880047267.01.T01:CDS
TGTTGATCTTAACATGGTTC+AGG 0.306649 8:+83976364 MsG0880047267.01.T01:CDS
GAATTCTGAATTCTCACATT+AGG 0.340007 8:-83976341 None:intergenic
TACTTCACATCATCATGTTA+TGG 0.361031 8:+83976100 MsG0880047267.01.T01:CDS
CAAGAGTTTGAGAAGGTAGT+AGG 0.384655 8:+83976260 MsG0880047267.01.T01:CDS
TGTTGTTGGATGCAAGTGTA+TGG 0.400360 8:+83976301 MsG0880047267.01.T01:CDS
GAGAAGGTAGTAGGTGTTGA+AGG 0.415252 8:+83976269 MsG0880047267.01.T01:CDS
TGATGTGAAGTAGAATCTTC+AGG 0.436223 8:-83976089 None:intergenic
GTTGATCTTAACATGGTTCA+GGG 0.438643 8:+83976365 MsG0880047267.01.T01:CDS
CCAATGCAGAGAGGGTTACT+TGG 0.447490 8:-83976410 None:intergenic
GAAGGTGAAGGTGTTGTTGT+TGG 0.461516 8:+83976287 MsG0880047267.01.T01:CDS
CCAAGTAACCCTCTCTGCAT+TGG 0.508307 8:+83976410 MsG0880047267.01.T01:CDS
AAAGAGGATTGAAGTTTGCA+TGG 0.512458 8:+83976199 MsG0880047267.01.T01:CDS
TGGAGTTGGTCTAGAGGATG+TGG 0.516198 8:+83976430 MsG0880047267.01.T01:CDS
AGGGGAGGATTACAAAGATG+TGG 0.525816 8:+83976475 MsG0880047267.01.T01:CDS
TGAAGTAGAATCTTCAGGTA+TGG 0.531707 8:-83976084 None:intergenic
AACCCTCTCTGCATTGGAGT+TGG 0.545052 8:+83976416 MsG0880047267.01.T01:CDS
CTGCATTGGAGTTGGTCTAG+AGG 0.545072 8:+83976424 MsG0880047267.01.T01:CDS
GTTGTTGGATGCAAGTGTAT+GGG 0.547984 8:+83976302 MsG0880047267.01.T01:CDS
ATAATGCATCTCTTGTTACT+GGG 0.551376 8:+83976138 MsG0880047267.01.T01:CDS
TTATCCCTGCAGCTCAAAAG+AGG 0.567917 8:+83976183 MsG0880047267.01.T01:CDS
AAGAGGATTGAAGTTTGCAT+GGG 0.571804 8:+83976200 MsG0880047267.01.T01:CDS
GACCAACTCCAATGCAGAGA+GGG 0.577245 8:-83976418 None:intergenic
TTGTTGGATGCAAGTGTATG+GGG 0.578412 8:+83976303 MsG0880047267.01.T01:CDS
ATGTGGATACTATTGTGGCT+AGG 0.581180 8:+83976447 MsG0880047267.01.T01:CDS
AGAGGATGTGGATACTATTG+TGG 0.582949 8:+83976442 MsG0880047267.01.T01:CDS
GTAGTAGGTGTTGAAGGTGA+AGG 0.594774 8:+83976275 MsG0880047267.01.T01:CDS
GGATTACAAAGATGTGGGTA+CGG 0.595227 8:+83976481 MsG0880047267.01.T01:CDS
GAATTCTGTTGATCTTAACA+TGG 0.597830 8:+83976358 MsG0880047267.01.T01:CDS
GGGGAGGATTACAAAGATGT+GGG 0.600350 8:+83976476 MsG0880047267.01.T01:CDS
TCTTCAGGTATGGATAGCAT+AGG 0.636532 8:-83976074 None:intergenic
ATTGTTACAAGAGTTTGAGA+AGG 0.637353 8:+83976253 MsG0880047267.01.T01:CDS
GTTTAACAAAGAGACTAATG+TGG 0.665239 8:+83976160 MsG0880047267.01.T01:CDS
AGACCAACTCCAATGCAGAG+AGG 0.696753 8:-83976419 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAACAAATGCAAAAAATCA+GGG + Chr8:83976222-83976241 MsG0880047267.01.T01:CDS 25.0%
! TAACAAATGCAAAAAATCAG+GGG + Chr8:83976223-83976242 MsG0880047267.01.T01:CDS 25.0%
AATAATGCATCTCTTGTTAC+TGG + Chr8:83976137-83976156 MsG0880047267.01.T01:CDS 30.0%
ATAATGCATCTCTTGTTACT+GGG + Chr8:83976138-83976157 MsG0880047267.01.T01:CDS 30.0%
ATTGTTACAAGAGTTTGAGA+AGG + Chr8:83976253-83976272 MsG0880047267.01.T01:CDS 30.0%
GAATTCTGAATTCTCACATT+AGG - Chr8:83976344-83976363 None:intergenic 30.0%
GAATTCTGTTGATCTTAACA+TGG + Chr8:83976358-83976377 MsG0880047267.01.T01:CDS 30.0%
GGTAACAAATGCAAAAAATC+AGG + Chr8:83976221-83976240 MsG0880047267.01.T01:CDS 30.0%
GTTTAACAAAGAGACTAATG+TGG + Chr8:83976160-83976179 MsG0880047267.01.T01:CDS 30.0%
TACTTCACATCATCATGTTA+TGG + Chr8:83976100-83976119 MsG0880047267.01.T01:CDS 30.0%
AAAGAGGATTGAAGTTTGCA+TGG + Chr8:83976199-83976218 MsG0880047267.01.T01:CDS 35.0%
AAGAGGATTGAAGTTTGCAT+GGG + Chr8:83976200-83976219 MsG0880047267.01.T01:CDS 35.0%
GTTGATCTTAACATGGTTCA+GGG + Chr8:83976365-83976384 MsG0880047267.01.T01:CDS 35.0%
TGAAGTAGAATCTTCAGGTA+TGG - Chr8:83976087-83976106 None:intergenic 35.0%
TGATGTGAAGTAGAATCTTC+AGG - Chr8:83976092-83976111 None:intergenic 35.0%
TGTTGATCTTAACATGGTTC+AGG + Chr8:83976364-83976383 MsG0880047267.01.T01:CDS 35.0%
! ACTATTGTGGCTAGGTTTTT+AGG + Chr8:83976455-83976474 MsG0880047267.01.T01:CDS 35.0%
! CTATTGTGGCTAGGTTTTTA+GGG + Chr8:83976456-83976475 MsG0880047267.01.T01:CDS 35.0%
!! TATTGTGGCTAGGTTTTTAG+GGG + Chr8:83976457-83976476 MsG0880047267.01.T01:CDS 35.0%
AGAGGATGTGGATACTATTG+TGG + Chr8:83976442-83976461 MsG0880047267.01.T01:CDS 40.0%
ATGTGGATACTATTGTGGCT+AGG + Chr8:83976447-83976466 MsG0880047267.01.T01:CDS 40.0%
CAAGAGTTTGAGAAGGTAGT+AGG + Chr8:83976260-83976279 MsG0880047267.01.T01:CDS 40.0%
GGATTACAAAGATGTGGGTA+CGG + Chr8:83976481-83976500 MsG0880047267.01.T01:CDS 40.0%
GTTGTTGGATGCAAGTGTAT+GGG + Chr8:83976302-83976321 MsG0880047267.01.T01:CDS 40.0%
TGTTGTTGGATGCAAGTGTA+TGG + Chr8:83976301-83976320 MsG0880047267.01.T01:CDS 40.0%
TTGTTGGATGCAAGTGTATG+GGG + Chr8:83976303-83976322 MsG0880047267.01.T01:CDS 40.0%
AGGGGAGGATTACAAAGATG+TGG + Chr8:83976475-83976494 MsG0880047267.01.T01:CDS 45.0%
GGGGAGGATTACAAAGATGT+GGG + Chr8:83976476-83976495 MsG0880047267.01.T01:CDS 45.0%
TTATCCCTGCAGCTCAAAAG+AGG + Chr8:83976183-83976202 MsG0880047267.01.T01:CDS 45.0%
! CAATCCTCTTTTGAGCTGCA+GGG - Chr8:83976190-83976209 None:intergenic 45.0%
! TCAATCCTCTTTTGAGCTGC+AGG - Chr8:83976191-83976210 None:intergenic 45.0%
!! GAAGGTGAAGGTGTTGTTGT+TGG + Chr8:83976287-83976306 MsG0880047267.01.T01:CDS 45.0%
!! GAGAAGGTAGTAGGTGTTGA+AGG + Chr8:83976269-83976288 MsG0880047267.01.T01:CDS 45.0%
!! GTAGTAGGTGTTGAAGGTGA+AGG + Chr8:83976275-83976294 MsG0880047267.01.T01:CDS 45.0%
AACCCTCTCTGCATTGGAGT+TGG + Chr8:83976416-83976435 MsG0880047267.01.T01:CDS 50.0%
AGACCAACTCCAATGCAGAG+AGG - Chr8:83976422-83976441 None:intergenic 50.0%
CCAAGTAACCCTCTCTGCAT+TGG + Chr8:83976410-83976429 MsG0880047267.01.T01:CDS 50.0%
CCAATGCAGAGAGGGTTACT+TGG - Chr8:83976413-83976432 None:intergenic 50.0%
GACCAACTCCAATGCAGAGA+GGG - Chr8:83976421-83976440 None:intergenic 50.0%
! CTGCATTGGAGTTGGTCTAG+AGG + Chr8:83976424-83976443 MsG0880047267.01.T01:CDS 50.0%
! TGGAGTTGGTCTAGAGGATG+TGG + Chr8:83976430-83976449 MsG0880047267.01.T01:CDS 50.0%
!! TGTGGCTAGGTTTTTAGGGG+AGG + Chr8:83976460-83976479 MsG0880047267.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 83976077 83976523 83976077 ID=MsG0880047267.01;Name=MsG0880047267.01
Chr8 mRNA 83976077 83976523 83976077 ID=MsG0880047267.01.T01;Parent=MsG0880047267.01;Name=MsG0880047267.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|148
Chr8 exon 83976077 83976523 83976077 ID=MsG0880047267.01.T01:exon:35845;Parent=MsG0880047267.01.T01
Chr8 CDS 83976077 83976523 83976077 ID=MsG0880047267.01.T01:cds;Parent=MsG0880047267.01.T01
Gene Sequence

>MsG0880047267.01.T01

ATGCTATCCATACCTGAAGATTCTACTTCACATCATCATGTTATGGATGTGTGCACTACAAATAATGCATCTCTTGTTACTGGGTTTAACAAAGAGACTAATGTGGTTATCCCTGCAGCTCAAAAGAGGATTGAAGTTTGCATGGGTAACAAATGCAAAAAATCAGGGGCTGCTACATTGTTACAAGAGTTTGAGAAGGTAGTAGGTGTTGAAGGTGAAGGTGTTGTTGTTGGATGCAAGTGTATGGGGAAGTGTAAAACTGCTCCTAATGTGAGAATTCAGAATTCTGTTGATCTTAACATGGTTCAGGGAATTGATGATTCTGTTAAAATTCCAAGTAACCCTCTCTGCATTGGAGTTGGTCTAGAGGATGTGGATACTATTGTGGCTAGGTTTTTAGGGGAGGATTACAAAGATGTGGGTACGGTTGCTGCAACTTCTTCATGA

Protein sequence

>MsG0880047267.01.T01

MLSIPEDSTSHHHVMDVCTTNNASLVTGFNKETNVVIPAAQKRIEVCMGNKCKKSGAATLLQEFEKVVGVEGEGVVVGCKCMGKCKTAPNVRIQNSVDLNMVQGIDDSVKIPSNPLCIGVGLEDVDTIVARFLGEDYKDVGTVAATSS*