Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025523.01.T01 | XP_003612898.1 | 93.939 | 99 | 6 | 0 | 1 | 99 | 22 | 120 | 3.57E-61 | 192 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025523.01.T01 | G7KF21 | 93.939 | 99 | 6 | 0 | 1 | 99 | 22 | 120 | 1.70e-61 | 192 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004396.01 | MsG0580025523.01 | 0.810510 | 1.112302e-50 | 6.717444e-48 |
| MsG0580025523.01 | MsG0580025548.01 | 0.875946 | 2.062907e-68 | 9.819981e-65 |
| MsG0580025523.01 | MsG0580025549.01 | 0.874797 | 5.097913e-68 | 2.326005e-64 |
| MsG0580025523.01 | MsG0580028021.01 | 0.810940 | 8.975699e-51 | 5.483952e-48 |
| MsG0580025523.01 | MsG0780041091.01 | 0.800081 | 1.703352e-48 | 7.859780e-46 |
| MsG0580025523.01 | MsG0880041999.01 | 0.809331 | 1.995064e-50 | 1.168029e-47 |
| MsG0580025523.01 | MsG0880045285.01 | 0.807071 | 6.044705e-50 | 3.334778e-47 |
| MsG0580025523.01 | MsG0880047267.01 | 0.809552 | 1.788057e-50 | 1.053040e-47 |
| MsG0380017293.01 | MsG0580025523.01 | 0.800278 | 1.553598e-48 | 7.204719e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025523.01.T01 | MTR_5g430430 | 93.939 | 99 | 6 | 0 | 1 | 99 | 22 | 120 | 4.32e-65 | 192 |
| MsG0580025523.01.T01 | MTR_5g030290 | 93.939 | 99 | 6 | 0 | 1 | 99 | 22 | 120 | 4.32e-65 | 192 |
| MsG0580025523.01.T01 | MTR_5g030310 | 84.848 | 99 | 15 | 0 | 1 | 99 | 22 | 120 | 7.98e-58 | 174 |
| MsG0580025523.01.T01 | MTR_5g430440 | 83.838 | 99 | 16 | 0 | 1 | 99 | 22 | 120 | 8.44e-57 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATTAATTTGTACTAAATT+TGG | 0.168371 | 5:-19973119 | MsG0580025523.01.T01:three_prime_UTR |
| TCACTTCTCTAGGGACAATT+TGG | 0.245955 | 5:+19973180 | None:intergenic |
| CTTGCTCTTGAGTGCAGCTT+TGG | 0.250449 | 5:+19974191 | None:intergenic |
| TCACTGCTACTCTCAATTTC+AGG | 0.257468 | 5:+19973342 | None:intergenic |
| CTCATATCATTGTGAGAATA+TGG | 0.263715 | 5:-19974218 | MsG0580025523.01.T01:five_prime_UTR |
| TTGCTCTTGAGTGCAGCTTT+GGG | 0.361214 | 5:+19974192 | None:intergenic |
| TGTGTGATCAGCTATGAATT+TGG | 0.395112 | 5:-19973080 | MsG0580025523.01.T01:three_prime_UTR |
| GTTTGTAATTAGTATGTGTT+TGG | 0.438400 | 5:-19973047 | MsG0580025523.01.T01:three_prime_UTR |
| CCCTTGCCTGAGTCTGCATC+AGG | 0.461443 | 5:+19973252 | None:intergenic |
| GTAATGCAATAGTATGTGTT+TGG | 0.464999 | 5:-19973009 | MsG0580025523.01.T01:three_prime_UTR |
| CCTTAGTGAATCACTTCTCT+AGG | 0.478513 | 5:+19973170 | None:intergenic |
| TTCAAGCTAATAAAATATCA+AGG | 0.479076 | 5:-19973143 | MsG0580025523.01.T01:three_prime_UTR |
| TACTAAATTTGGTTTATCAT+CGG | 0.484979 | 5:-19973108 | MsG0580025523.01.T01:three_prime_UTR |
| TGTGTTATAACTAGGACTAC+TGG | 0.487878 | 5:-19973935 | MsG0580025523.01.T01:CDS |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.489184 | 5:-19973971 | MsG0580025523.01.T01:CDS |
| TAAACCAATAAGCTTTGTGC+AGG | 0.493645 | 5:-19974033 | MsG0580025523.01.T01:intron |
| GAGGTAGAGCCTTGAAAAGT+CGG | 0.524080 | 5:+19974303 | None:intergenic |
| CCTAGAGAAGTGATTCACTA+AGG | 0.524929 | 5:-19973170 | MsG0580025523.01.T01:three_prime_UTR |
| AATGTATCAACTAGTGAATG+AGG | 0.527069 | 5:+19974284 | None:intergenic |
| ATAAGCTTTGTGCAGGAAAG+TGG | 0.528788 | 5:-19974026 | MsG0580025523.01.T01:intron |
| AGGACTACTGGACCTGATGC+TGG | 0.536873 | 5:-19973923 | MsG0580025523.01.T01:CDS |
| CAAAGCTGCACTCAAGAGCA+AGG | 0.561178 | 5:-19974190 | MsG0580025523.01.T01:five_prime_UTR |
| TCCTGATGCAGACTCAGGCA+AGG | 0.574927 | 5:-19973253 | MsG0580025523.01.T01:CDS |
| GAAGGTCCTGATGCAGACTC+AGG | 0.576340 | 5:-19973258 | MsG0580025523.01.T01:CDS |
| CTAGAGAAGTGATTCACTAA+GGG | 0.578373 | 5:-19973169 | MsG0580025523.01.T01:three_prime_UTR |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5:-19973943 | MsG0580025523.01.T01:CDS |
| TCTGCAAGTGTGGTGAAGGA+TGG | 0.591787 | 5:-19973302 | MsG0580025523.01.T01:CDS |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.593456 | 5:-19973306 | MsG0580025523.01.T01:CDS |
| CTTAGTGAATCACTTCTCTA+GGG | 0.599847 | 5:+19973171 | None:intergenic |
| TCTTGTGCATGCAGAGATGG+TGG | 0.610106 | 5:-19973375 | MsG0580025523.01.T01:intron |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.620823 | 5:-19973336 | MsG0580025523.01.T01:CDS |
| GTGTGTGACATACAGTGTGC+AGG | 0.624270 | 5:+19973868 | None:intergenic |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5:-19973965 | MsG0580025523.01.T01:CDS |
| CCTGATGCAGACTCAGGCAA+GGG | 0.633802 | 5:-19973252 | MsG0580025523.01.T01:CDS |
| AAAGCTGCACTCAAGAGCAA+GGG | 0.647133 | 5:-19974189 | MsG0580025523.01.T01:intron |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5:-19973961 | MsG0580025523.01.T01:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5:-19973312 | MsG0580025523.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTAATTTGTACTAAATT+TGG | - | Chr5:19974130-19974149 | MsG0580025523.01.T01:intron | 10.0% |
| !! | AAAACTATATAGATGAAATA+AGG | + | Chr5:19973113-19973132 | None:intergenic | 15.0% |
| !! | GATTTATTTATGTATGAAAA+TGG | + | Chr5:19973191-19973210 | None:intergenic | 15.0% |
| !!! | AAAGTATTATAGTTCATTTT+TGG | - | Chr5:19973845-19973864 | MsG0580025523.01.T01:intron | 15.0% |
| !! | ATTAACTCTTGAGATAAAAT+CGG | - | Chr5:19973752-19973771 | MsG0580025523.01.T01:intron | 20.0% |
| !! | GATGGTAGTAAAATTAAAAT+AGG | + | Chr5:19973418-19973437 | None:intergenic | 20.0% |
| !! | GTAAAATTAAAATAGGCAAA+AGG | + | Chr5:19973411-19973430 | None:intergenic | 20.0% |
| !! | TTCAAGCTAATAAAATATCA+AGG | - | Chr5:19974106-19974125 | MsG0580025523.01.T01:intron | 20.0% |
| !!! | TACTAAATTTGGTTTATCAT+CGG | - | Chr5:19974141-19974160 | MsG0580025523.01.T01:intron | 20.0% |
| !!! | TTTTTCAAAATCGAATGAAT+TGG | - | Chr5:19973485-19973504 | MsG0580025523.01.T01:intron | 20.0% |
| ! | AATGAAACTGCAATGTAATA+AGG | + | Chr5:19973561-19973580 | None:intergenic | 25.0% |
| ! | ATTATATAAGCCTAGATTTG+TGG | + | Chr5:19973803-19973822 | None:intergenic | 25.0% |
| ! | GTTTGTAATTAGTATGTGTT+TGG | - | Chr5:19974202-19974221 | MsG0580025523.01.T01:five_prime_UTR | 25.0% |
| ! | TGTTGTAACAATAGTTGAAT+CGG | + | Chr5:19973690-19973709 | None:intergenic | 25.0% |
| ! | TTTGATCAAAATCATTTCGA+TGG | + | Chr5:19973436-19973455 | None:intergenic | 25.0% |
| AAAATGGAACAAGAGTAGTA+TGG | + | Chr5:19973175-19973194 | None:intergenic | 30.0% | |
| AAATGGAACAAGAGTAGTAT+GGG | + | Chr5:19973174-19973193 | None:intergenic | 30.0% | |
| AATAGTACTCTGGATCAAAT+CGG | + | Chr5:19973722-19973741 | None:intergenic | 30.0% | |
| AATGTATCAACTAGTGAATG+AGG | + | Chr5:19972968-19972987 | None:intergenic | 30.0% | |
| AGATTTGTGGCATATTTGAA+TGG | + | Chr5:19973790-19973809 | None:intergenic | 30.0% | |
| CTCATATCATTGTGAGAATA+TGG | - | Chr5:19973031-19973050 | MsG0580025523.01.T01:three_prime_UTR | 30.0% | |
| GTAATGCAATAGTATGTGTT+TGG | - | Chr5:19974240-19974259 | MsG0580025523.01.T01:five_prime_UTR | 30.0% | |
| TCAAAATCGAATGAATTGGT+TGG | - | Chr5:19973489-19973508 | MsG0580025523.01.T01:intron | 30.0% | |
| TCTGAAAAACAATAGTACTC+TGG | + | Chr5:19973732-19973751 | None:intergenic | 30.0% | |
| TTCAAATATGCCACAAATCT+AGG | - | Chr5:19973790-19973809 | MsG0580025523.01.T01:intron | 30.0% | |
| ! | GGCAAAGTTTTTGTTAAATG+CGG | - | Chr5:19973347-19973366 | MsG0580025523.01.T01:CDS | 30.0% |
| !! | GAAATTTTAAGAGACAACAG+AGG | + | Chr5:19974292-19974311 | None:intergenic | 30.0% |
| CTAGAGAAGTGATTCACTAA+GGG | - | Chr5:19974080-19974099 | MsG0580025523.01.T01:intron | 35.0% | |
| CTTAGTGAATCACTTCTCTA+GGG | + | Chr5:19974081-19974100 | None:intergenic | 35.0% | |
| GTAGTTCAAGACAAAAACTC+AGG | - | Chr5:19973245-19973264 | MsG0580025523.01.T01:CDS | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | Chr5:19973306-19973325 | MsG0580025523.01.T01:CDS | 35.0% | |
| TGTGTGATCAGCTATGAATT+TGG | - | Chr5:19974169-19974188 | MsG0580025523.01.T01:intron | 35.0% | |
| TGTGTTATAACTAGGACTAC+TGG | - | Chr5:19973314-19973333 | MsG0580025523.01.T01:CDS | 35.0% | |
| ! | AATGAATTGGTTGGTTCAAC+TGG | - | Chr5:19973498-19973517 | MsG0580025523.01.T01:intron | 35.0% |
| ! | TAAACCAATAAGCTTTGTGC+AGG | - | Chr5:19973216-19973235 | MsG0580025523.01.T01:CDS | 35.0% |
| !! | GATGGAGTTGTTCCATTTTT+AGG | - | Chr5:19973965-19973984 | MsG0580025523.01.T01:CDS | 35.0% |
| !!! | TGTTCCATTTTTAGGACTGA+AGG | - | Chr5:19973973-19973992 | MsG0580025523.01.T01:CDS | 35.0% |
| ACAAAAACTTTGCCAGCATC+AGG | + | Chr5:19973341-19973360 | None:intergenic | 40.0% | |
| AGGACCTTCAGTCCTAAAAA+TGG | + | Chr5:19973980-19973999 | None:intergenic | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | Chr5:19973937-19973956 | MsG0580025523.01.T01:CDS | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | Chr5:19973913-19973932 | MsG0580025523.01.T01:CDS | 40.0% | |
| CAAGACAAAAACTCAGGCAT+GGG | - | Chr5:19973251-19973270 | MsG0580025523.01.T01:CDS | 40.0% | |
| CCTAGAGAAGTGATTCACTA+AGG | - | Chr5:19974079-19974098 | MsG0580025523.01.T01:intron | 40.0% | |
| CCTTAGTGAATCACTTCTCT+AGG | + | Chr5:19974082-19974101 | None:intergenic | 40.0% | |
| CTTTCCTGCACAAAGCTTAT+TGG | + | Chr5:19973223-19973242 | None:intergenic | 40.0% | |
| TCAAGACAAAAACTCAGGCA+TGG | - | Chr5:19973250-19973269 | MsG0580025523.01.T01:CDS | 40.0% | |
| TCACTGCTACTCTCAATTTC+AGG | + | Chr5:19973910-19973929 | None:intergenic | 40.0% | |
| TGAGCTGACAACAGTTGAAA+GGG | + | Chr5:19973608-19973627 | None:intergenic | 40.0% | |
| TTGAGCTGACAACAGTTGAA+AGG | + | Chr5:19973609-19973628 | None:intergenic | 40.0% | |
| ! | ATAAGCTTTGTGCAGGAAAG+TGG | - | Chr5:19973223-19973242 | MsG0580025523.01.T01:CDS | 40.0% |
| ! | TCACTTCTCTAGGGACAATT+TGG | + | Chr5:19974072-19974091 | None:intergenic | 40.0% |
| AAAAACTCAGGCATGGGCAT+GGG | - | Chr5:19973257-19973276 | MsG0580025523.01.T01:CDS | 45.0% | |
| AAAGCTGCACTCAAGAGCAA+GGG | - | Chr5:19973060-19973079 | MsG0580025523.01.T01:three_prime_UTR | 45.0% | |
| AACTCTTGTGCATGCAGAGA+TGG | - | Chr5:19973871-19973890 | MsG0580025523.01.T01:intron | 45.0% | |
| ACATTCTGCAAGTGTGGTGA+AGG | - | Chr5:19973943-19973962 | MsG0580025523.01.T01:CDS | 45.0% | |
| GAGGTAGAGCCTTGAAAAGT+CGG | + | Chr5:19972949-19972968 | None:intergenic | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | - | Chr5:19973284-19973303 | MsG0580025523.01.T01:CDS | 45.0% | |
| TGGAACAAGAGTAGTATGGG+TGG | + | Chr5:19973171-19973190 | None:intergenic | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | - | Chr5:19973288-19973307 | MsG0580025523.01.T01:CDS | 45.0% | |
| TTGCTCTTGAGTGCAGCTTT+GGG | + | Chr5:19973060-19973079 | None:intergenic | 45.0% | |
| CAAAAACTCAGGCATGGGCA+TGG | - | Chr5:19973256-19973275 | MsG0580025523.01.T01:CDS | 50.0% | |
| CAAAGCTGCACTCAAGAGCA+AGG | - | Chr5:19973059-19973078 | MsG0580025523.01.T01:three_prime_UTR | 50.0% | |
| CTTGCTCTTGAGTGCAGCTT+TGG | + | Chr5:19973061-19973080 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | Chr5:19973278-19973297 | MsG0580025523.01.T01:CDS | 50.0% | |
| GTGTGTGACATACAGTGTGC+AGG | + | Chr5:19973384-19973403 | None:intergenic | 50.0% | |
| TCTGCAAGTGTGGTGAAGGA+TGG | - | Chr5:19973947-19973966 | MsG0580025523.01.T01:CDS | 50.0% | |
| TCTTGTGCATGCAGAGATGG+TGG | - | Chr5:19973874-19973893 | MsG0580025523.01.T01:intron | 50.0% | |
| AGGACTACTGGACCTGATGC+TGG | - | Chr5:19973326-19973345 | MsG0580025523.01.T01:CDS | 55.0% | |
| CCTGATGCAGACTCAGGCAA+GGG | - | Chr5:19973997-19974016 | MsG0580025523.01.T01:exon | 55.0% | |
| GAAGGTCCTGATGCAGACTC+AGG | - | Chr5:19973991-19974010 | MsG0580025523.01.T01:exon | 55.0% | |
| TCCTGATGCAGACTCAGGCA+AGG | - | Chr5:19973996-19974015 | MsG0580025523.01.T01:exon | 55.0% | |
| CCCTTGCCTGAGTCTGCATC+AGG | + | Chr5:19974000-19974019 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 19972941 | 19974330 | 19972941 | ID=MsG0580025523.01;Name=MsG0580025523.01 |
| Chr5 | mRNA | 19972941 | 19974330 | 19972941 | ID=MsG0580025523.01.T01;Parent=MsG0580025523.01;Name=MsG0580025523.01.T01;_AED=0.30;_eAED=0.30;_QI=182|0.5|0.66|1|0.5|0.33|3|265|99 |
| Chr5 | exon | 19972941 | 19973383 | 19972941 | ID=MsG0580025523.01.T01:exon:8399;Parent=MsG0580025523.01.T01 |
| Chr5 | exon | 19973881 | 19974043 | 19973881 | ID=MsG0580025523.01.T01:exon:8398;Parent=MsG0580025523.01.T01 |
| Chr5 | exon | 19974190 | 19974330 | 19974190 | ID=MsG0580025523.01.T01:exon:8397;Parent=MsG0580025523.01.T01 |
| Chr5 | five_prime_UTR | 19974190 | 19974330 | 19974190 | ID=MsG0580025523.01.T01:five_prime_utr;Parent=MsG0580025523.01.T01 |
| Chr5 | five_prime_UTR | 19974003 | 19974043 | 19974003 | ID=MsG0580025523.01.T01:five_prime_utr;Parent=MsG0580025523.01.T01 |
| Chr5 | CDS | 19973881 | 19974002 | 19973881 | ID=MsG0580025523.01.T01:cds;Parent=MsG0580025523.01.T01 |
| Chr5 | CDS | 19973206 | 19973383 | 19973206 | ID=MsG0580025523.01.T01:cds;Parent=MsG0580025523.01.T01 |
| Chr5 | three_prime_UTR | 19972941 | 19973205 | 19972941 | ID=MsG0580025523.01.T01:three_prime_utr;Parent=MsG0580025523.01.T01 |
| Gene Sequence |
| Protein sequence |
