Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025609.01.T01 | XP_003612984.1 | 95.58 | 362 | 16 | 0 | 1 | 362 | 1 | 362 | 0 | 694 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025609.01.T01 | Q9ZRF1 | 79.666 | 359 | 70 | 1 | 1 | 359 | 1 | 356 | 0 | 566 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025609.01.T01 | G7KGI7 | 95.580 | 362 | 16 | 0 | 1 | 362 | 1 | 362 | 0.0 | 694 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022090.01 | MsG0580025609.01 | 0.807537 | 4.815134e-50 | 2.688837e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025609.01.T01 | MTR_5g031310 | 95.580 | 362 | 16 | 0 | 1 | 362 | 1 | 362 | 0.0 | 694 |
| MsG0580025609.01.T01 | MTR_5g031305 | 92.818 | 362 | 26 | 0 | 1 | 362 | 1 | 362 | 0.0 | 682 |
| MsG0580025609.01.T01 | MTR_5g031360 | 87.430 | 358 | 45 | 0 | 5 | 362 | 6 | 363 | 0.0 | 629 |
| MsG0580025609.01.T01 | MTR_5g031300 | 83.934 | 361 | 58 | 0 | 1 | 361 | 1 | 361 | 0.0 | 625 |
| MsG0580025609.01.T01 | MTR_5g031230 | 81.994 | 361 | 64 | 1 | 1 | 361 | 1 | 360 | 0.0 | 601 |
| MsG0580025609.01.T01 | MTR_4g115300 | 83.286 | 353 | 59 | 0 | 9 | 361 | 8 | 360 | 0.0 | 601 |
| MsG0580025609.01.T01 | MTR_5g031390 | 83.003 | 353 | 60 | 0 | 9 | 361 | 8 | 360 | 0.0 | 590 |
| MsG0580025609.01.T01 | MTR_5g031250 | 80.609 | 361 | 69 | 1 | 1 | 361 | 1 | 360 | 0.0 | 582 |
| MsG0580025609.01.T01 | MTR_5g031210 | 79.603 | 353 | 72 | 0 | 9 | 361 | 8 | 360 | 0.0 | 565 |
| MsG0580025609.01.T01 | MTR_5g031190 | 76.271 | 354 | 84 | 0 | 8 | 361 | 7 | 360 | 0.0 | 559 |
| MsG0580025609.01.T01 | MTR_5g031180 | 80.737 | 353 | 68 | 0 | 9 | 361 | 8 | 360 | 0.0 | 553 |
| MsG0580025609.01.T01 | MTR_8g068190 | 78.470 | 353 | 76 | 0 | 9 | 361 | 8 | 360 | 0.0 | 538 |
| MsG0580025609.01.T01 | MTR_8g092630 | 61.602 | 362 | 133 | 2 | 2 | 358 | 53 | 413 | 6.48e-153 | 437 |
| MsG0580025609.01.T01 | MTR_5g031430 | 60.580 | 345 | 136 | 0 | 15 | 359 | 14 | 358 | 1.44e-146 | 419 |
| MsG0580025609.01.T01 | MTR_5g031250 | 81.818 | 242 | 43 | 1 | 1 | 242 | 1 | 241 | 9.47e-141 | 400 |
| MsG0580025609.01.T01 | MTR_1g025950 | 57.184 | 348 | 148 | 1 | 10 | 356 | 8 | 355 | 3.07e-133 | 385 |
| MsG0580025609.01.T01 | MTR_1g107425 | 50.729 | 343 | 169 | 0 | 12 | 354 | 9 | 351 | 3.38e-121 | 354 |
| MsG0580025609.01.T01 | MTR_2g009810 | 53.890 | 347 | 156 | 3 | 15 | 359 | 62 | 406 | 1.61e-117 | 347 |
| MsG0580025609.01.T01 | MTR_1g107425 | 50.865 | 289 | 141 | 1 | 12 | 299 | 9 | 297 | 5.17e-96 | 288 |
| MsG0580025609.01.T01 | MTR_2g009820 | 51.724 | 232 | 109 | 2 | 131 | 359 | 23 | 254 | 9.62e-63 | 201 |
| MsG0580025609.01.T01 | MTR_5g031290 | 79.130 | 115 | 24 | 0 | 248 | 362 | 80 | 194 | 4.55e-57 | 184 |
| MsG0580025609.01.T01 | MTR_5g031290 | 82.716 | 81 | 12 | 2 | 1 | 80 | 1 | 80 | 7.20e-41 | 142 |
| MsG0580025609.01.T01 | MTR_0001s0660 | 28.947 | 304 | 188 | 10 | 39 | 319 | 35 | 333 | 1.28e-22 | 99.0 |
| MsG0580025609.01.T01 | MTR_8g095220 | 26.599 | 297 | 186 | 8 | 11 | 279 | 51 | 343 | 3.36e-22 | 97.4 |
| MsG0580025609.01.T01 | MTR_8g095220 | 28.125 | 256 | 157 | 7 | 11 | 243 | 51 | 302 | 6.07e-21 | 92.4 |
| MsG0580025609.01.T01 | MTR_3g089977 | 24.339 | 378 | 242 | 14 | 12 | 355 | 12 | 379 | 1.66e-17 | 83.2 |
| MsG0580025609.01.T01 | MTR_3g089940 | 24.728 | 368 | 234 | 13 | 12 | 345 | 12 | 370 | 2.51e-17 | 82.8 |
| MsG0580025609.01.T01 | MTR_3g089940 | 24.457 | 368 | 235 | 13 | 12 | 345 | 12 | 370 | 4.67e-17 | 82.0 |
| MsG0580025609.01.T01 | MTR_6g465010 | 25.271 | 277 | 182 | 9 | 26 | 289 | 13 | 277 | 1.24e-16 | 80.5 |
| MsG0580025609.01.T01 | MTR_6g466320 | 24.910 | 277 | 183 | 8 | 26 | 289 | 29 | 293 | 1.57e-16 | 80.5 |
| MsG0580025609.01.T01 | MTR_6g465010 | 24.910 | 277 | 183 | 8 | 26 | 289 | 29 | 293 | 1.57e-16 | 80.5 |
| MsG0580025609.01.T01 | MTR_7g068320 | 26.488 | 336 | 211 | 14 | 40 | 341 | 48 | 381 | 5.04e-16 | 79.0 |
| MsG0580025609.01.T01 | MTR_3g089940 | 25.000 | 336 | 217 | 11 | 12 | 320 | 12 | 339 | 7.47e-16 | 78.2 |
| MsG0580025609.01.T01 | MTR_3g089940 | 24.702 | 336 | 218 | 11 | 12 | 320 | 12 | 339 | 1.13e-15 | 77.8 |
| MsG0580025609.01.T01 | MTR_3g089970 | 24.044 | 366 | 235 | 13 | 14 | 345 | 15 | 371 | 2.29e-15 | 77.0 |
| MsG0580025609.01.T01 | MTR_7g099040 | 25.360 | 347 | 217 | 12 | 12 | 328 | 13 | 347 | 7.28e-14 | 72.4 |
| MsG0580025609.01.T01 | MTR_5g045710 | 24.405 | 336 | 221 | 10 | 39 | 345 | 35 | 366 | 9.85e-14 | 72.0 |
| MsG0580025609.01.T01 | MTR_7g068330 | 26.786 | 336 | 209 | 14 | 40 | 341 | 41 | 373 | 1.24e-13 | 72.0 |
| MsG0580025609.01.T01 | MTR_1g069430 | 26.239 | 343 | 211 | 14 | 1 | 313 | 1 | 331 | 1.85e-13 | 71.2 |
| MsG0580025609.01.T01 | MTR_5g031380 | 64.103 | 39 | 14 | 0 | 81 | 119 | 19 | 57 | 2.26e-11 | 60.8 |
| MsG0580025609.01.T01 | MTR_3g099460 | 25.641 | 312 | 196 | 11 | 40 | 320 | 37 | 343 | 2.77e-11 | 64.7 |
| MsG0580025609.01.T01 | MTR_3g099460 | 25.641 | 312 | 196 | 11 | 40 | 320 | 44 | 350 | 3.20e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025609.01.T01 | AT4G37980 | 72.080 | 351 | 98 | 0 | 12 | 362 | 7 | 357 | 6.55e-177 | 495 |
| MsG0580025609.01.T01 | AT4G37990 | 72.080 | 351 | 98 | 0 | 12 | 362 | 7 | 357 | 1.01e-173 | 488 |
| MsG0580025609.01.T01 | AT4G37970 | 63.739 | 353 | 128 | 0 | 9 | 361 | 9 | 361 | 1.11e-165 | 467 |
| MsG0580025609.01.T01 | AT4G39330 | 62.040 | 353 | 134 | 0 | 8 | 360 | 7 | 359 | 2.57e-162 | 459 |
| MsG0580025609.01.T01 | AT2G21730 | 60.857 | 350 | 135 | 2 | 13 | 360 | 6 | 355 | 2.46e-151 | 431 |
| MsG0580025609.01.T01 | AT2G21890 | 60.172 | 349 | 138 | 1 | 13 | 360 | 6 | 354 | 2.62e-151 | 431 |
| MsG0580025609.01.T01 | AT4G37980 | 70.548 | 292 | 86 | 0 | 12 | 303 | 7 | 298 | 1.32e-135 | 389 |
| MsG0580025609.01.T01 | AT4G39330 | 61.279 | 297 | 115 | 0 | 8 | 304 | 7 | 303 | 1.76e-130 | 376 |
| MsG0580025609.01.T01 | AT4G37970 | 62.069 | 290 | 110 | 0 | 9 | 298 | 9 | 298 | 2.19e-128 | 370 |
| MsG0580025609.01.T01 | AT1G72680 | 50.437 | 343 | 169 | 1 | 17 | 359 | 14 | 355 | 2.42e-114 | 337 |
| MsG0580025609.01.T01 | AT3G19450 | 51.734 | 346 | 167 | 0 | 12 | 357 | 9 | 354 | 4.84e-113 | 333 |
| MsG0580025609.01.T01 | AT4G34230 | 50.575 | 348 | 172 | 0 | 7 | 354 | 3 | 350 | 9.50e-107 | 317 |
| MsG0580025609.01.T01 | AT4G34230 | 50.287 | 348 | 173 | 0 | 7 | 354 | 3 | 350 | 7.50e-106 | 315 |
| MsG0580025609.01.T01 | AT4G37970 | 60.759 | 237 | 93 | 0 | 9 | 245 | 9 | 245 | 9.67e-101 | 298 |
| MsG0580025609.01.T01 | AT4G37970 | 61.277 | 235 | 91 | 0 | 9 | 243 | 9 | 243 | 1.58e-100 | 298 |
| MsG0580025609.01.T01 | AT5G63620 | 26.207 | 290 | 186 | 6 | 16 | 279 | 58 | 345 | 2.64e-23 | 100 |
| MsG0580025609.01.T01 | AT5G63620 | 26.207 | 290 | 186 | 6 | 16 | 279 | 58 | 345 | 2.82e-23 | 100 |
| MsG0580025609.01.T01 | AT5G63620 | 27.711 | 249 | 157 | 5 | 16 | 243 | 58 | 304 | 3.87e-22 | 95.9 |
| MsG0580025609.01.T01 | AT1G64710 | 26.790 | 377 | 235 | 16 | 1 | 343 | 1 | 370 | 2.75e-18 | 85.9 |
| MsG0580025609.01.T01 | AT1G64710 | 26.923 | 338 | 210 | 14 | 40 | 343 | 13 | 347 | 3.79e-18 | 85.1 |
| MsG0580025609.01.T01 | AT5G43940 | 26.048 | 334 | 210 | 10 | 40 | 344 | 36 | 361 | 4.34e-18 | 85.1 |
| MsG0580025609.01.T01 | AT5G43940 | 26.048 | 334 | 210 | 10 | 40 | 344 | 48 | 373 | 5.35e-18 | 85.1 |
| MsG0580025609.01.T01 | AT1G22430 | 25.369 | 339 | 214 | 11 | 40 | 343 | 42 | 376 | 1.65e-17 | 83.6 |
| MsG0580025609.01.T01 | AT1G22430 | 25.369 | 339 | 214 | 11 | 40 | 343 | 42 | 376 | 1.65e-17 | 83.6 |
| MsG0580025609.01.T01 | AT5G51970 | 25.882 | 255 | 168 | 6 | 37 | 279 | 40 | 285 | 7.66e-17 | 81.3 |
| MsG0580025609.01.T01 | AT5G51970 | 25.882 | 255 | 168 | 6 | 37 | 279 | 40 | 285 | 7.66e-17 | 81.3 |
| MsG0580025609.01.T01 | AT1G32780 | 27.348 | 362 | 216 | 15 | 40 | 359 | 37 | 393 | 1.54e-16 | 80.9 |
| MsG0580025609.01.T01 | AT1G77120 | 23.262 | 374 | 251 | 12 | 12 | 355 | 11 | 378 | 2.54e-14 | 73.9 |
| MsG0580025609.01.T01 | AT4G22110 | 25.075 | 335 | 215 | 13 | 40 | 344 | 43 | 371 | 9.22e-14 | 72.4 |
| MsG0580025609.01.T01 | AT4G22110 | 25.075 | 335 | 215 | 13 | 40 | 344 | 43 | 371 | 9.22e-14 | 72.4 |
| MsG0580025609.01.T01 | AT1G22430 | 26.000 | 200 | 129 | 5 | 40 | 222 | 42 | 239 | 4.03e-13 | 69.7 |
| MsG0580025609.01.T01 | AT1G22430 | 26.000 | 200 | 129 | 5 | 40 | 222 | 42 | 239 | 6.16e-13 | 68.9 |
| MsG0580025609.01.T01 | AT1G22430 | 26.000 | 200 | 129 | 5 | 40 | 222 | 42 | 239 | 6.16e-13 | 68.9 |
Find 86 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAGTCCTCTTCGACATTT+TGG | 0.197168 | 5:-21208936 | MsG0580025609.01.T01:CDS |
| GTGAAATTCGCCAAAGCTTT+TGG | 0.225325 | 5:-21208852 | MsG0580025609.01.T01:CDS |
| TATCGATTCGTGATTGATAT+TGG | 0.245698 | 5:-21208193 | MsG0580025609.01.T01:CDS |
| ATTGGTTTATTGAAATCTAA+TGG | 0.271827 | 5:-21208566 | MsG0580025609.01.T01:CDS |
| TCCTGCAATGTTTAAGGGCT+TGG | 0.287864 | 5:+21208157 | None:intergenic |
| AAGTTTGATTGGAGGGATAA+AGG | 0.302889 | 5:-21208332 | MsG0580025609.01.T01:CDS |
| TCTTGGTGGACTTGGTCATA+TGG | 0.309264 | 5:-21208877 | MsG0580025609.01.T01:CDS |
| CCCATTCATTCTTCATCATA+TGG | 0.323377 | 5:+21209578 | None:intergenic |
| TTAGTAGCTGGAAGTTTGAT+TGG | 0.337701 | 5:-21208343 | MsG0580025609.01.T01:CDS |
| ACCACAAGTGACTGTTTGTT+GGG | 0.342426 | 5:+21209097 | None:intergenic |
| CATCAAAACTTTAGGGAAAC+TGG | 0.347590 | 5:-21209655 | MsG0580025609.01.T01:intron |
| TGGTATTATCGACACAGTTT+CGG | 0.347787 | 5:-21208612 | MsG0580025609.01.T01:CDS |
| GCCCAACCAAAGGCCTTCTT+AGG | 0.362894 | 5:+21209927 | None:intergenic |
| CACATTAGCGCCAAAAGCTT+TGG | 0.364310 | 5:+21208842 | None:intergenic |
| GCCGGTCCTCTTCTTTGTGC+TGG | 0.364538 | 5:-21208969 | MsG0580025609.01.T01:CDS |
| CCCTGGAACTAATGGATAGA+TGG | 0.366233 | 5:+21209549 | None:intergenic |
| ATAGGAATAACCTCAATATC+AGG | 0.384692 | 5:+21208265 | None:intergenic |
| TCCATCTATCCATTAGTTCC+AGG | 0.385414 | 5:-21209550 | MsG0580025609.01.T01:CDS |
| AACTTACCTCTTGATGTTGC+CGG | 0.388597 | 5:-21208987 | MsG0580025609.01.T01:CDS |
| TCCTAAGAAGGCCTTTGGTT+GGG | 0.390941 | 5:-21209928 | MsG0580025609.01.T01:CDS |
| ACATCAAGAGGTAAGTTATC+AGG | 0.391560 | 5:+21208993 | None:intergenic |
| AAAGTAGAGAAGTTTAAAGT+TGG | 0.393389 | 5:-21209200 | MsG0580025609.01.T01:CDS |
| GTTAGGCGATGTACTAATTA+CGG | 0.396532 | 5:+21208818 | None:intergenic |
| CACCACAAGTGACTGTTTGT+TGG | 0.396853 | 5:+21209096 | None:intergenic |
| GTAGGGAGAAAGTTAGTAGC+TGG | 0.412852 | 5:-21208355 | MsG0580025609.01.T01:intron |
| CCCCTTTCAATTTCTCCAGA+AGG | 0.415467 | 5:-21209878 | MsG0580025609.01.T01:intron |
| AAATGCAGGCATGAACTTGC+GGG | 0.418681 | 5:-21209245 | MsG0580025609.01.T01:intron |
| CGTGATGGCACTGTCACATA+TGG | 0.422522 | 5:-21209065 | MsG0580025609.01.T01:CDS |
| ATAGGCGTTGTTGGTCTTGG+TGG | 0.424569 | 5:-21208891 | MsG0580025609.01.T01:CDS |
| GAAGCATACCCTGGAACTAA+TGG | 0.426302 | 5:+21209541 | None:intergenic |
| GTTGTTGGTCTTGGTGGACT+TGG | 0.427259 | 5:-21208885 | MsG0580025609.01.T01:CDS |
| GAGCATCCTAAGAAGGCCTT+TGG | 0.429666 | 5:-21209933 | MsG0580025609.01.T01:CDS |
| TCCATATGATGAAGAATGAA+TGG | 0.431554 | 5:-21209579 | MsG0580025609.01.T01:CDS |
| CATTCAAGGTGTTGTATTGT+GGG | 0.442854 | 5:-21209618 | MsG0580025609.01.T01:CDS |
| TTTAAAGTTGGAGACAAAGT+TGG | 0.442932 | 5:-21209188 | MsG0580025609.01.T01:CDS |
| AATATAGGCGTTGTTGGTCT+TGG | 0.444452 | 5:-21208894 | MsG0580025609.01.T01:CDS |
| CTCTACTTTGCTTCCAACCT+CGG | 0.449103 | 5:+21209214 | None:intergenic |
| GACAGTGCCATCACGATACT+TGG | 0.451051 | 5:+21209073 | None:intergenic |
| TGGGCAGCTAGAGATCCTTC+TGG | 0.459135 | 5:-21209909 | MsG0580025609.01.T01:CDS |
| TGAGAAAGATGTAGCATTCA+AGG | 0.467351 | 5:-21209632 | MsG0580025609.01.T01:CDS |
| GCTGTGTTGACATAATCAAT+AGG | 0.469010 | 5:+21208247 | None:intergenic |
| CCATATGATGAAGAATGAAT+GGG | 0.478005 | 5:-21209578 | MsG0580025609.01.T01:CDS |
| CCTCCAAGTAAAGAAAATAC+AGG | 0.487127 | 5:+21208500 | None:intergenic |
| GATTTCAATAAACCAATCAA+TGG | 0.487235 | 5:+21208572 | None:intergenic |
| GGTATGAATATAGGCGTTGT+TGG | 0.488041 | 5:-21208900 | MsG0580025609.01.T01:CDS |
| CAATGTAAAGCCTGATATTG+AGG | 0.489359 | 5:-21208275 | MsG0580025609.01.T01:CDS |
| TGGAGGTTATTCTGACTCAA+TGG | 0.494148 | 5:-21209045 | MsG0580025609.01.T01:CDS |
| TAGCTGGAAGTTTGATTGGA+GGG | 0.499663 | 5:-21208339 | MsG0580025609.01.T01:CDS |
| ACCTTCTGGAGAAATTGAAA+GGG | 0.500807 | 5:+21209877 | None:intergenic |
| ATCCTAAGAAGGCCTTTGGT+TGG | 0.502470 | 5:-21209929 | MsG0580025609.01.T01:CDS |
| AACCTTCTGGAGAAATTGAA+AGG | 0.504412 | 5:+21209876 | None:intergenic |
| GACAAGCCTGGTATGAATAT+AGG | 0.507608 | 5:-21208909 | MsG0580025609.01.T01:CDS |
| AAAGGAAGCAATAGAACACT+TGG | 0.509890 | 5:-21208790 | MsG0580025609.01.T01:CDS |
| GCATTCAAGGTGTTGTATTG+TGG | 0.522756 | 5:-21209619 | MsG0580025609.01.T01:CDS |
| GTAGCTGGAAGTTTGATTGG+AGG | 0.522925 | 5:-21208340 | MsG0580025609.01.T01:CDS |
| CAAAGTTGGTGTGGGCTACA+TGG | 0.524993 | 5:-21209174 | MsG0580025609.01.T01:CDS |
| AGTGTCAGAGCATCCTAAGA+AGG | 0.533747 | 5:-21209940 | MsG0580025609.01.T01:CDS |
| TACATCGCCTAACAAAGAAA+AGG | 0.540053 | 5:-21208808 | MsG0580025609.01.T01:CDS |
| GAAAATACAGGAAGCTCTAG+AGG | 0.546279 | 5:+21208512 | None:intergenic |
| CCATCTATCCATTAGTTCCA+GGG | 0.551121 | 5:-21209549 | MsG0580025609.01.T01:intron |
| TCCAGCACAAAGAAGAGGAC+CGG | 0.566097 | 5:+21208968 | None:intergenic |
| GCTGCAATGAGTACTTTGAA+TGG | 0.568095 | 5:-21208632 | MsG0580025609.01.T01:CDS |
| TAAATGCAGGCATGAACTTG+CGG | 0.570766 | 5:-21209246 | MsG0580025609.01.T01:intron |
| ATCTCTAGCTGCCCAACCAA+AGG | 0.576625 | 5:+21209917 | None:intergenic |
| AGTTGGAGACAAAGTTGGTG+TGG | 0.579016 | 5:-21209183 | MsG0580025609.01.T01:CDS |
| ATCTAATGGAAAGCTTGTAA+TGG | 0.587781 | 5:-21208552 | MsG0580025609.01.T01:CDS |
| CCGCGATTCAGATCAGATGC+AGG | 0.590600 | 5:-21208745 | MsG0580025609.01.T01:intron |
| CCACAAGTGACTGTTTGTTG+GGG | 0.595949 | 5:+21209098 | None:intergenic |
| TGATTATGTCAACACAGCAA+TGG | 0.603631 | 5:-21208242 | MsG0580025609.01.T01:CDS |
| GTGACTCCAGCACAAAGAAG+AGG | 0.605674 | 5:+21208963 | None:intergenic |
| GCGGGTATAGTAACCGAGGT+TGG | 0.608625 | 5:-21209227 | MsG0580025609.01.T01:CDS |
| GTTGGAGACAAAGTTGGTGT+GGG | 0.610808 | 5:-21209182 | MsG0580025609.01.T01:CDS |
| AGAGGACCGGCAACATCAAG+AGG | 0.610980 | 5:+21208981 | None:intergenic |
| AATGGAAAGCTTGTAATGGT+TGG | 0.618001 | 5:-21208548 | MsG0580025609.01.T01:CDS |
| AAAGGGGAGAGAACACCAGA+AGG | 0.635132 | 5:+21209894 | None:intergenic |
| TCGAGTCCAAAATGTCGAAG+AGG | 0.639224 | 5:+21208930 | None:intergenic |
| GATGGCACTGTCACATATGG+AGG | 0.647077 | 5:-21209062 | MsG0580025609.01.T01:CDS |
| CCTTCTGGAGAAATTGAAAG+GGG | 0.647504 | 5:+21209878 | None:intergenic |
| ACAACGCCTATATTCATACC+AGG | 0.648632 | 5:+21208903 | None:intergenic |
| CACAAGTGACTGTTTGTTGG+GGG | 0.661357 | 5:+21209099 | None:intergenic |
| CATATGATGAAGAATGAATG+GGG | 0.667164 | 5:-21209577 | MsG0580025609.01.T01:CDS |
| AAGGAAGCAATAGAACACTT+GGG | 0.667331 | 5:-21208789 | MsG0580025609.01.T01:CDS |
| CCTGCATCTGATCTGAATCG+CGG | 0.667814 | 5:+21208745 | None:intergenic |
| CCCCAACAAACAGTCACTTG+TGG | 0.684195 | 5:-21209098 | MsG0580025609.01.T01:CDS |
| TGTGGTGCCAAGTATCGTGA+TGG | 0.688725 | 5:-21209080 | MsG0580025609.01.T01:CDS |
| ACTTGCGGGTATAGTAACCG+AGG | 0.754285 | 5:-21209231 | MsG0580025609.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATATTAATTTCACATTAT+TGG | - | Chr5:21209748-21209767 | MsG0580025609.01.T01:intron | 10.0% |
| !!! | ATATTTTATTTAATAAATGC+AGG | - | Chr5:21208866-21208885 | MsG0580025609.01.T01:CDS | 10.0% |
| !! | AGAAATGAATAGTAAATAAT+TGG | - | Chr5:21208832-21208851 | MsG0580025609.01.T01:CDS | 15.0% |
| !! | TAAACATCAAATATTATTTC+AGG | + | Chr5:21208384-21208403 | None:intergenic | 15.0% |
| !! | TTAAAGTAAGTATAAAGATA+TGG | + | Chr5:21208727-21208746 | None:intergenic | 15.0% |
| !!! | AATATTGCATAACATTTTAT+AGG | - | Chr5:21209471-21209490 | MsG0580025609.01.T01:intron | 15.0% |
| !! | AAGGAATGTTAATATTATCA+AGG | - | Chr5:21208357-21208376 | MsG0580025609.01.T01:intron | 20.0% |
| !! | ATTAATTTCACATTATTGGT+AGG | - | Chr5:21209752-21209771 | MsG0580025609.01.T01:intron | 20.0% |
| !! | TTAATTTCACATTATTGGTA+GGG | - | Chr5:21209753-21209772 | MsG0580025609.01.T01:intron | 20.0% |
| !! | TTCAAGTAAGTATAAAGATA+TGG | + | Chr5:21208655-21208674 | None:intergenic | 20.0% |
| !!! | ATCAACTCATCTTTATTTAA+TGG | - | Chr5:21208284-21208303 | MsG0580025609.01.T01:CDS | 20.0% |
| !!! | ATTGGTTTATTGAAATCTAA+TGG | - | Chr5:21209559-21209578 | MsG0580025609.01.T01:CDS | 20.0% |
| !!! | TTAGTCTTAAACTATAGTAT+GGG | + | Chr5:21209682-21209701 | None:intergenic | 20.0% |
| !!! | TTTAGTCTTAAACTATAGTA+TGG | + | Chr5:21209683-21209702 | None:intergenic | 20.0% |
| ! | AATGCAACAAATAATGAGTT+AGG | + | Chr5:21208315-21208334 | None:intergenic | 25.0% |
| ! | GATTTCAATAAACCAATCAA+TGG | + | Chr5:21209556-21209575 | None:intergenic | 25.0% |
| ! | TAAACTACAATTGTAGTAGA+AGG | + | Chr5:21208615-21208634 | None:intergenic | 25.0% |
| ! | TTATAATGAAGTAAACCTTC+TGG | + | Chr5:21208265-21208284 | None:intergenic | 25.0% |
| !! | AAAGTAGAGAAGTTTAAAGT+TGG | - | Chr5:21208925-21208944 | MsG0580025609.01.T01:CDS | 25.0% |
| !!! | TCAAGAAATGATTGATTTTG+CGG | - | Chr5:21209820-21209839 | MsG0580025609.01.T01:intron | 25.0% |
| AACATTCCTTGAAAACTAAC+AGG | + | Chr5:21208347-21208366 | None:intergenic | 30.0% | |
| ACATTCCTTGAAAACTAACA+GGG | + | Chr5:21208346-21208365 | None:intergenic | 30.0% | |
| ATAGGAATAACCTCAATATC+AGG | + | Chr5:21209863-21209882 | None:intergenic | 30.0% | |
| ATCTAATGGAAAGCTTGTAA+TGG | - | Chr5:21209573-21209592 | MsG0580025609.01.T01:CDS | 30.0% | |
| CATATGATGAAGAATGAATG+GGG | - | Chr5:21208548-21208567 | MsG0580025609.01.T01:CDS | 30.0% | |
| CCATATGATGAAGAATGAAT+GGG | - | Chr5:21208547-21208566 | MsG0580025609.01.T01:CDS | 30.0% | |
| GCTACTAATTAAGTCAACTT+TGG | - | Chr5:21208445-21208464 | MsG0580025609.01.T01:intron | 30.0% | |
| TAGTTTATTCCTACGTTACT+TGG | - | Chr5:21208628-21208647 | MsG0580025609.01.T01:CDS | 30.0% | |
| TCCATATGATGAAGAATGAA+TGG | - | Chr5:21208546-21208565 | MsG0580025609.01.T01:CDS | 30.0% | |
| ! | ATTGCTTCCTTTTCTTTGTT+AGG | + | Chr5:21209327-21209346 | None:intergenic | 30.0% |
| ! | CATTCTGTTTTACCATTGAT+TGG | - | Chr5:21209541-21209560 | MsG0580025609.01.T01:intron | 30.0% |
| ! | CTATAGTATGGGAGTATATT+TGG | + | Chr5:21209671-21209690 | None:intergenic | 30.0% |
| ! | CTTCCTGTATTTTCTTTACT+TGG | - | Chr5:21209622-21209641 | MsG0580025609.01.T01:CDS | 30.0% |
| ! | TTTAAAGTTGGAGACAAAGT+TGG | - | Chr5:21208937-21208956 | MsG0580025609.01.T01:CDS | 30.0% |
| ! | TTTGGTGCATCAAAACTTTA+GGG | - | Chr5:21208463-21208482 | MsG0580025609.01.T01:intron | 30.0% |
| !! | TATCGATTCGTGATTGATAT+TGG | - | Chr5:21209932-21209951 | MsG0580025609.01.T01:CDS | 30.0% |
| !!! | TTTTTCCCTGTTAGTTTTCA+AGG | - | Chr5:21208338-21208357 | MsG0580025609.01.T01:CDS | 30.0% |
| AAAGGAAGCAATAGAACACT+TGG | - | Chr5:21209335-21209354 | MsG0580025609.01.T01:intron | 35.0% | |
| AAGGAAGCAATAGAACACTT+GGG | - | Chr5:21209336-21209355 | MsG0580025609.01.T01:intron | 35.0% | |
| AAGTTTGATTGGAGGGATAA+AGG | - | Chr5:21209793-21209812 | MsG0580025609.01.T01:intron | 35.0% | |
| AATGGAAAGCTTGTAATGGT+TGG | - | Chr5:21209577-21209596 | MsG0580025609.01.T01:CDS | 35.0% | |
| ACAATTGTAGTAGAAGGAGT+TGG | + | Chr5:21208609-21208628 | None:intergenic | 35.0% | |
| ACATCAAGAGGTAAGTTATC+AGG | + | Chr5:21209135-21209154 | None:intergenic | 35.0% | |
| CAATGTAAAGCCTGATATTG+AGG | - | Chr5:21209850-21209869 | MsG0580025609.01.T01:intron | 35.0% | |
| CCCATTCATTCTTCATCATA+TGG | + | Chr5:21208550-21208569 | None:intergenic | 35.0% | |
| CCTCCAAGTAAAGAAAATAC+AGG | + | Chr5:21209628-21209647 | None:intergenic | 35.0% | |
| GTTAGGCGATGTACTAATTA+CGG | + | Chr5:21209310-21209329 | None:intergenic | 35.0% | |
| TACATCGCCTAACAAAGAAA+AGG | - | Chr5:21209317-21209336 | MsG0580025609.01.T01:intron | 35.0% | |
| TGAGAAAGATGTAGCATTCA+AGG | - | Chr5:21208493-21208512 | MsG0580025609.01.T01:intron | 35.0% | |
| TGATTATGTCAACACAGCAA+TGG | - | Chr5:21209883-21209902 | MsG0580025609.01.T01:CDS | 35.0% | |
| TGGTATTATCGACACAGTTT+CGG | - | Chr5:21209513-21209532 | MsG0580025609.01.T01:intron | 35.0% | |
| TTAGTAGCTGGAAGTTTGAT+TGG | - | Chr5:21209782-21209801 | MsG0580025609.01.T01:intron | 35.0% | |
| ! | AACCTTCTGGAGAAATTGAA+AGG | + | Chr5:21208252-21208271 | None:intergenic | 35.0% |
| ! | ACCTTCTGGAGAAATTGAAA+GGG | + | Chr5:21208251-21208270 | None:intergenic | 35.0% |
| ! | CATCAAAACTTTAGGGAAAC+TGG | - | Chr5:21208470-21208489 | MsG0580025609.01.T01:intron | 35.0% |
| ! | CCTGTATTTTCTTTACTTGG+AGG | - | Chr5:21209625-21209644 | MsG0580025609.01.T01:CDS | 35.0% |
| ! | CTTTGGTGCATCAAAACTTT+AGG | - | Chr5:21208462-21208481 | MsG0580025609.01.T01:intron | 35.0% |
| ! | GCTGTGTTGACATAATCAAT+AGG | + | Chr5:21209881-21209900 | None:intergenic | 35.0% |
| ! | TATAGTCCTCTTCGACATTT+TGG | - | Chr5:21209189-21209208 | MsG0580025609.01.T01:CDS | 35.0% |
| !! | CATTCAAGGTGTTGTATTGT+GGG | - | Chr5:21208507-21208526 | MsG0580025609.01.T01:CDS | 35.0% |
| AACTTACCTCTTGATGTTGC+CGG | - | Chr5:21209138-21209157 | MsG0580025609.01.T01:CDS | 40.0% | |
| ACAACGCCTATATTCATACC+AGG | + | Chr5:21209225-21209244 | None:intergenic | 40.0% | |
| ACCACAAGTGACTGTTTGTT+GGG | + | Chr5:21209031-21209050 | None:intergenic | 40.0% | |
| AGATATGGTCCAAGTAACGT+AGG | + | Chr5:21208640-21208659 | None:intergenic | 40.0% | |
| CCATCTATCCATTAGTTCCA+GGG | - | Chr5:21208576-21208595 | MsG0580025609.01.T01:CDS | 40.0% | |
| GACAAGCCTGGTATGAATAT+AGG | - | Chr5:21209216-21209235 | MsG0580025609.01.T01:CDS | 40.0% | |
| GCTGCAATGAGTACTTTGAA+TGG | - | Chr5:21209493-21209512 | MsG0580025609.01.T01:intron | 40.0% | |
| TAAATGCAGGCATGAACTTG+CGG | - | Chr5:21208879-21208898 | MsG0580025609.01.T01:CDS | 40.0% | |
| TAGCTGGAAGTTTGATTGGA+GGG | - | Chr5:21209786-21209805 | MsG0580025609.01.T01:intron | 40.0% | |
| TCCATCTATCCATTAGTTCC+AGG | - | Chr5:21208575-21208594 | MsG0580025609.01.T01:CDS | 40.0% | |
| TGGAGGTTATTCTGACTCAA+TGG | - | Chr5:21209080-21209099 | MsG0580025609.01.T01:CDS | 40.0% | |
| ! | AATATAGGCGTTGTTGGTCT+TGG | - | Chr5:21209231-21209250 | MsG0580025609.01.T01:CDS | 40.0% |
| ! | CCTTCTGGAGAAATTGAAAG+GGG | + | Chr5:21208250-21208269 | None:intergenic | 40.0% |
| ! | GAAAATACAGGAAGCTCTAG+AGG | + | Chr5:21209616-21209635 | None:intergenic | 40.0% |
| ! | GGTATGAATATAGGCGTTGT+TGG | - | Chr5:21209225-21209244 | MsG0580025609.01.T01:CDS | 40.0% |
| ! | GTGAAATTCGCCAAAGCTTT+TGG | - | Chr5:21209273-21209292 | MsG0580025609.01.T01:intron | 40.0% |
| !! | GCATTCAAGGTGTTGTATTG+TGG | - | Chr5:21208506-21208525 | MsG0580025609.01.T01:CDS | 40.0% |
| AAATGCAGGCATGAACTTGC+GGG | - | Chr5:21208880-21208899 | MsG0580025609.01.T01:CDS | 45.0% | |
| ATCCTAAGAAGGCCTTTGGT+TGG | - | Chr5:21208196-21208215 | MsG0580025609.01.T01:CDS | 45.0% | |
| CACATTAGCGCCAAAAGCTT+TGG | + | Chr5:21209286-21209305 | None:intergenic | 45.0% | |
| CACCACAAGTGACTGTTTGT+TGG | + | Chr5:21209032-21209051 | None:intergenic | 45.0% | |
| CCCCTTTCAATTTCTCCAGA+AGG | - | Chr5:21208247-21208266 | MsG0580025609.01.T01:CDS | 45.0% | |
| CTCTACTTTGCTTCCAACCT+CGG | + | Chr5:21208914-21208933 | None:intergenic | 45.0% | |
| GAAGCATACCCTGGAACTAA+TGG | + | Chr5:21208587-21208606 | None:intergenic | 45.0% | |
| GTAGCTGGAAGTTTGATTGG+AGG | - | Chr5:21209785-21209804 | MsG0580025609.01.T01:intron | 45.0% | |
| GTAGGGAGAAAGTTAGTAGC+TGG | - | Chr5:21209770-21209789 | MsG0580025609.01.T01:intron | 45.0% | |
| TCCTAAGAAGGCCTTTGGTT+GGG | - | Chr5:21208197-21208216 | MsG0580025609.01.T01:CDS | 45.0% | |
| TCGAGTCCAAAATGTCGAAG+AGG | + | Chr5:21209198-21209217 | None:intergenic | 45.0% | |
| ! | AGTGTCAGAGCATCCTAAGA+AGG | - | Chr5:21208185-21208204 | MsG0580025609.01.T01:CDS | 45.0% |
| ! | CACAAGTGACTGTTTGTTGG+GGG | + | Chr5:21209029-21209048 | None:intergenic | 45.0% |
| ! | CCACAAGTGACTGTTTGTTG+GGG | + | Chr5:21209030-21209049 | None:intergenic | 45.0% |
| ! | CCCTGGAACTAATGGATAGA+TGG | + | Chr5:21208579-21208598 | None:intergenic | 45.0% |
| !! | AGTTGGAGACAAAGTTGGTG+TGG | - | Chr5:21208942-21208961 | MsG0580025609.01.T01:CDS | 45.0% |
| !! | GTTGGAGACAAAGTTGGTGT+GGG | - | Chr5:21208943-21208962 | MsG0580025609.01.T01:CDS | 45.0% |
| !! | TCTTGGTGGACTTGGTCATA+TGG | - | Chr5:21209248-21209267 | MsG0580025609.01.T01:intron | 45.0% |
| AAAGGGGAGAGAACACCAGA+AGG | + | Chr5:21208234-21208253 | None:intergenic | 50.0% | |
| ACTTGCGGGTATAGTAACCG+AGG | - | Chr5:21208894-21208913 | MsG0580025609.01.T01:CDS | 50.0% | |
| ATCTCTAGCTGCCCAACCAA+AGG | + | Chr5:21208211-21208230 | None:intergenic | 50.0% | |
| CCCCAACAAACAGTCACTTG+TGG | - | Chr5:21209027-21209046 | MsG0580025609.01.T01:CDS | 50.0% | |
| CCTGCATCTGATCTGAATCG+CGG | + | Chr5:21209383-21209402 | None:intergenic | 50.0% | |
| CGTGATGGCACTGTCACATA+TGG | - | Chr5:21209060-21209079 | MsG0580025609.01.T01:CDS | 50.0% | |
| GACAGTGCCATCACGATACT+TGG | + | Chr5:21209055-21209074 | None:intergenic | 50.0% | |
| GAGCATCCTAAGAAGGCCTT+TGG | - | Chr5:21208192-21208211 | MsG0580025609.01.T01:CDS | 50.0% | |
| GATGGCACTGTCACATATGG+AGG | - | Chr5:21209063-21209082 | MsG0580025609.01.T01:CDS | 50.0% | |
| GTGACTCCAGCACAAAGAAG+AGG | + | Chr5:21209165-21209184 | None:intergenic | 50.0% | |
| TCCAGCACAAAGAAGAGGAC+CGG | + | Chr5:21209160-21209179 | None:intergenic | 50.0% | |
| ! | AAGGAGTTGGAAGCATACCC+TGG | + | Chr5:21208596-21208615 | None:intergenic | 50.0% |
| ! | CATTTTGGACTCGACAAGCC+TGG | - | Chr5:21209204-21209223 | MsG0580025609.01.T01:CDS | 50.0% |
| ! | TGTGGTGCCAAGTATCGTGA+TGG | - | Chr5:21209045-21209064 | MsG0580025609.01.T01:CDS | 50.0% |
| !! | ATAGGCGTTGTTGGTCTTGG+TGG | - | Chr5:21209234-21209253 | MsG0580025609.01.T01:CDS | 50.0% |
| !! | CAAAGTTGGTGTGGGCTACA+TGG | - | Chr5:21208951-21208970 | MsG0580025609.01.T01:CDS | 50.0% |
| !! | GTTGTTGGTCTTGGTGGACT+TGG | - | Chr5:21209240-21209259 | MsG0580025609.01.T01:CDS | 50.0% |
| AGAGGACCGGCAACATCAAG+AGG | + | Chr5:21209147-21209166 | None:intergenic | 55.0% | |
| CCGCGATTCAGATCAGATGC+AGG | - | Chr5:21209380-21209399 | MsG0580025609.01.T01:intron | 55.0% | |
| GCCCAACCAAAGGCCTTCTT+AGG | + | Chr5:21208201-21208220 | None:intergenic | 55.0% | |
| GCGGGTATAGTAACCGAGGT+TGG | - | Chr5:21208898-21208917 | MsG0580025609.01.T01:CDS | 55.0% | |
| TGGGCAGCTAGAGATCCTTC+TGG | - | Chr5:21208216-21208235 | MsG0580025609.01.T01:CDS | 55.0% | |
| GCCGGTCCTCTTCTTTGTGC+TGG | - | Chr5:21209156-21209175 | MsG0580025609.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 21208168 | 21209979 | 21208168 | ID=MsG0580025609.01;Name=MsG0580025609.01 |
| Chr5 | mRNA | 21208168 | 21209979 | 21208168 | ID=MsG0580025609.01.T01;Parent=MsG0580025609.01;Name=MsG0580025609.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|5|0|362 |
| Chr5 | exon | 21209879 | 21209979 | 21209879 | ID=MsG0580025609.01.T01:exon:23608;Parent=MsG0580025609.01.T01 |
| Chr5 | exon | 21209550 | 21209663 | 21209550 | ID=MsG0580025609.01.T01:exon:23607;Parent=MsG0580025609.01.T01 |
| Chr5 | exon | 21208746 | 21209259 | 21208746 | ID=MsG0580025609.01.T01:exon:23606;Parent=MsG0580025609.01.T01 |
| Chr5 | exon | 21208501 | 21208654 | 21208501 | ID=MsG0580025609.01.T01:exon:23605;Parent=MsG0580025609.01.T01 |
| Chr5 | exon | 21208168 | 21208373 | 21208168 | ID=MsG0580025609.01.T01:exon:23604;Parent=MsG0580025609.01.T01 |
| Chr5 | CDS | 21209879 | 21209979 | 21209879 | ID=MsG0580025609.01.T01:cds;Parent=MsG0580025609.01.T01 |
| Chr5 | CDS | 21209550 | 21209663 | 21209550 | ID=MsG0580025609.01.T01:cds;Parent=MsG0580025609.01.T01 |
| Chr5 | CDS | 21208746 | 21209259 | 21208746 | ID=MsG0580025609.01.T01:cds;Parent=MsG0580025609.01.T01 |
| Chr5 | CDS | 21208501 | 21208654 | 21208501 | ID=MsG0580025609.01.T01:cds;Parent=MsG0580025609.01.T01 |
| Chr5 | CDS | 21208168 | 21208373 | 21208168 | ID=MsG0580025609.01.T01:cds;Parent=MsG0580025609.01.T01 |
| Gene Sequence |
| Protein sequence |
