Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025753.01.T01 | RHN55037.1 | 99.487 | 195 | 1 | 0 | 1 | 195 | 1 | 195 | 4.25E-142 | 404 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025753.01.T01 | Q9LDT3 | 63.793 | 174 | 55 | 2 | 2 | 167 | 9 | 182 | 4.20E-66 | 205 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025753.01.T01 | A0A396HWH4 | 99.487 | 195 | 1 | 0 | 1 | 195 | 1 | 195 | 2.03e-142 | 404 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580025753.01.T01 | TF | C2C2-YABBY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000784.01 | MsG0580025753.01 | 0.819438 | 1.161102e-52 | 8.935110e-50 |
| MsG0180002929.01 | MsG0580025753.01 | 0.830607 | 2.670428e-55 | 2.827443e-52 |
| MsG0280010569.01 | MsG0580025753.01 | 0.806263 | 8.953800e-50 | 4.838472e-47 |
| MsG0280010611.01 | MsG0580025753.01 | 0.817745 | 2.811573e-52 | 2.064904e-49 |
| MsG0480020315.01 | MsG0580025753.01 | 0.804346 | 2.257554e-49 | 1.161261e-46 |
| MsG0480021060.01 | MsG0580025753.01 | 0.814692 | 1.353728e-51 | 9.145755e-49 |
| MsG0480021894.01 | MsG0580025753.01 | 0.805871 | 1.082666e-49 | 5.792195e-47 |
| MsG0580025753.01 | MsG0580025828.01 | 0.814227 | 1.715434e-51 | 1.144460e-48 |
| MsG0580025753.01 | MsG0580027726.01 | 0.818443 | 1.954662e-52 | 1.463518e-49 |
| MsG0580025753.01 | MsG0580027763.01 | 0.801933 | 7.128058e-49 | 3.446947e-46 |
| MsG0580025753.01 | MsG0780041262.01 | 0.801901 | 7.235772e-49 | 3.496347e-46 |
| MsG0580025753.01 | MsG0880045668.01 | 0.815070 | 1.115862e-51 | 7.615988e-49 |
| MsG0380017423.01 | MsG0580025753.01 | 0.803545 | 3.313071e-49 | 1.669537e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025753.01.T01 | MTR_5g034030 | 99.482 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 2.79e-144 | 400 |
| MsG0580025753.01.T01 | MTR_4g114730 | 49.686 | 159 | 58 | 4 | 12 | 162 | 14 | 158 | 4.44e-45 | 148 |
| MsG0580025753.01.T01 | MTR_2g087740 | 51.250 | 160 | 62 | 4 | 12 | 162 | 12 | 164 | 1.78e-44 | 146 |
| MsG0580025753.01.T01 | MTR_4g050300 | 48.344 | 151 | 73 | 1 | 12 | 162 | 9 | 154 | 1.86e-41 | 138 |
| MsG0580025753.01.T01 | MTR_4g025900 | 50.000 | 156 | 70 | 2 | 14 | 162 | 25 | 179 | 2.41e-40 | 137 |
| MsG0580025753.01.T01 | MTR_4g101660 | 51.553 | 161 | 64 | 5 | 12 | 162 | 18 | 174 | 8.39e-40 | 135 |
| MsG0580025753.01.T01 | MTR_5g046230 | 40.373 | 161 | 67 | 3 | 12 | 163 | 17 | 157 | 6.22e-30 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025753.01.T01 | AT1G23420 | 63.793 | 174 | 55 | 2 | 2 | 167 | 9 | 182 | 4.29e-67 | 205 |
| MsG0580025753.01.T01 | AT1G23420 | 63.793 | 174 | 55 | 2 | 2 | 167 | 40 | 213 | 9.61e-67 | 205 |
| MsG0580025753.01.T01 | AT2G26580 | 50.327 | 153 | 60 | 3 | 12 | 162 | 9 | 147 | 5.54e-47 | 152 |
| MsG0580025753.01.T01 | AT2G26580 | 50.327 | 153 | 60 | 3 | 12 | 162 | 9 | 147 | 5.54e-47 | 152 |
| MsG0580025753.01.T01 | AT2G45190 | 46.061 | 165 | 79 | 1 | 8 | 162 | 19 | 183 | 2.99e-43 | 144 |
| MsG0580025753.01.T01 | AT1G08465 | 49.677 | 155 | 66 | 4 | 12 | 162 | 8 | 154 | 2.23e-41 | 138 |
| MsG0580025753.01.T01 | AT4G00180 | 45.810 | 179 | 71 | 5 | 8 | 162 | 19 | 195 | 3.40e-39 | 134 |
| MsG0580025753.01.T01 | AT4G00180 | 43.976 | 166 | 67 | 5 | 21 | 162 | 1 | 164 | 2.38e-30 | 110 |
| MsG0580025753.01.T01 | AT1G69180 | 40.385 | 156 | 73 | 4 | 12 | 163 | 19 | 158 | 1.96e-29 | 107 |
| MsG0580025753.01.T01 | AT2G45190 | 63.636 | 66 | 24 | 0 | 97 | 162 | 54 | 119 | 1.12e-25 | 97.8 |
Find 29 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGCAGAGAAGAGATTAAA+AGG | 0.220244 | 5:+23745496 | MsG0580025753.01.T01:intron |
| CATCACCTTTGCACTGAGTT+GGG | 0.247070 | 5:-23745759 | None:intergenic |
| GCTTCCTTGTGAGCCATATC+AGG | 0.254990 | 5:-23745534 | None:intergenic |
| ACTCATGGTTACCATTAAAA+TGG | 0.296363 | 5:-23744749 | None:intergenic |
| GATGAGTAAGAGATGCTAAA+AGG | 0.346967 | 5:-23744983 | None:intergenic |
| GGTGTTCTTTGTCTCTTCTC+TGG | 0.372695 | 5:-23745366 | None:intergenic |
| TGTACATAGCAAATCTGTTC+TGG | 0.376697 | 5:-23744709 | None:intergenic |
| TCATCACCTTTGCACTGAGT+TGG | 0.384038 | 5:-23745760 | None:intergenic |
| TTATTGACAACATTGCTGAT+TGG | 0.429525 | 5:-23745234 | None:intergenic |
| ATCACCTTTGCACTGAGTTG+GGG | 0.440172 | 5:-23745758 | None:intergenic |
| GATCCACAAATTGATCTGTC+TGG | 0.455209 | 5:-23745795 | None:intergenic |
| AGCATCTCTTACTCATCTTG+AGG | 0.521370 | 5:+23744989 | MsG0580025753.01.T01:CDS |
| ACAATCAGAATAAGTCATCA+TGG | 0.529221 | 5:-23745193 | None:intergenic |
| TAGCCAGACAGATCAATTTG+TGG | 0.529249 | 5:+23745792 | MsG0580025753.01.T01:CDS |
| AGAAGAGATTAAAAGGCTAA+AGG | 0.529887 | 5:+23745503 | MsG0580025753.01.T01:CDS |
| CTTCCTTGTGAGCCATATCA+GGG | 0.545406 | 5:-23745533 | None:intergenic |
| GAGATGCTAAAAGGTGGAAA+GGG | 0.553103 | 5:-23744974 | None:intergenic |
| AGAGATGCTAAAAGGTGGAA+AGG | 0.557063 | 5:-23744975 | None:intergenic |
| GAGTAAGAGATGCTAAAAGG+TGG | 0.562368 | 5:-23744980 | None:intergenic |
| ACCATTGTCAAACTGCTGCA+TGG | 0.563063 | 5:-23744885 | None:intergenic |
| AAACCCTGATATGGCTCACA+AGG | 0.577441 | 5:+23745530 | MsG0580025753.01.T01:CDS |
| TCACCTTTGCACTGAGTTGG+GGG | 0.601505 | 5:-23745757 | None:intergenic |
| GATGACTTATTCTGATTGTG+AGG | 0.620697 | 5:+23745197 | MsG0580025753.01.T01:CDS |
| CAGATTTGCTATGTACAATG+TGG | 0.632418 | 5:+23744715 | MsG0580025753.01.T01:CDS |
| TCCATGCAGCAGTTTGACAA+TGG | 0.655787 | 5:+23744884 | MsG0580025753.01.T01:CDS |
| ATGAAGCGATTATAAGCTGA+TGG | 0.658312 | 5:-23745387 | None:intergenic |
| TGTGGATCTTGACTCCCAAG+TGG | 0.667148 | 5:+23745810 | MsG0580025753.01.T01:CDS |
| ATGCAGCAGTTTGACAATGG+TGG | 0.669140 | 5:+23744887 | MsG0580025753.01.T01:CDS |
| ATGGTGGTGACAGTAAGATG+TGG | 0.764759 | 5:+23744903 | MsG0580025753.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATTTTTAATTTTCAGT+GGG | + | Chr5:23745726-23745745 | MsG0580025753.01.T01:intron | 10.0% |
| !!! | TTTTTATTTTTAATTTTCAG+TGG | + | Chr5:23745725-23745744 | MsG0580025753.01.T01:intron | 10.0% |
| !! | AAAGAAAATAAACAAAGTAT+TGG | - | Chr5:23745037-23745056 | None:intergenic | 15.0% |
| !!! | TATAGGAAATTTTATAAGAT+TGG | + | Chr5:23745667-23745686 | MsG0580025753.01.T01:intron | 15.0% |
| !!! | TTGTTTATTTTCTTTTAGTT+AGG | + | Chr5:23745042-23745061 | MsG0580025753.01.T01:intron | 15.0% |
| ! | CTTCTTAACTTCATCATATT+TGG | + | Chr5:23744784-23744803 | MsG0580025753.01.T01:intron | 25.0% |
| ! | TCAACTCATTTGAAAACTAT+AGG | + | Chr5:23745650-23745669 | MsG0580025753.01.T01:intron | 25.0% |
| !! | ATTCTGCAATACCATTTTAA+TGG | + | Chr5:23744738-23744757 | MsG0580025753.01.T01:CDS | 25.0% |
| AAGAGCAATTAGAAAACTCA+TGG | - | Chr5:23744767-23744786 | None:intergenic | 30.0% | |
| AATGCAGAGAAGAGATTAAA+AGG | + | Chr5:23745496-23745515 | MsG0580025753.01.T01:intron | 30.0% | |
| ACAATCAGAATAAGTCATCA+TGG | - | Chr5:23745196-23745215 | None:intergenic | 30.0% | |
| ACTCATGGTTACCATTAAAA+TGG | - | Chr5:23744752-23744771 | None:intergenic | 30.0% | |
| AGAAGAGATTAAAAGGCTAA+AGG | + | Chr5:23745503-23745522 | MsG0580025753.01.T01:CDS | 30.0% | |
| TTATTGACAACATTGCTGAT+TGG | - | Chr5:23745237-23745256 | None:intergenic | 30.0% | |
| !!! | TAGCTAGTTAGTTTTGAACA+TGG | - | Chr5:23745625-23745644 | None:intergenic | 30.0% |
| AAAGGCTAAAAACCCTGATA+TGG | + | Chr5:23745521-23745540 | MsG0580025753.01.T01:CDS | 35.0% | |
| ATGAAGCGATTATAAGCTGA+TGG | - | Chr5:23745390-23745409 | None:intergenic | 35.0% | |
| GATGACTTATTCTGATTGTG+AGG | + | Chr5:23745197-23745216 | MsG0580025753.01.T01:CDS | 35.0% | |
| GATGAGTAAGAGATGCTAAA+AGG | - | Chr5:23744986-23745005 | None:intergenic | 35.0% | |
| GTACAGCTGCAAAAAATGTA+AGG | + | Chr5:23745562-23745581 | MsG0580025753.01.T01:intron | 35.0% | |
| TCATTTCAATTGTGTGATGC+AGG | + | Chr5:23744854-23744873 | MsG0580025753.01.T01:intron | 35.0% | |
| TGGTGACTTACTCATAATCT+TGG | + | Chr5:23745687-23745706 | MsG0580025753.01.T01:intron | 35.0% | |
| TGTACATAGCAAATCTGTTC+TGG | - | Chr5:23744712-23744731 | None:intergenic | 35.0% | |
| !! | ATGTTTTTACCTCAGCTTCA+TGG | - | Chr5:23745840-23745859 | None:intergenic | 35.0% |
| !! | CAGATTTGCTATGTACAATG+TGG | + | Chr5:23744715-23744734 | MsG0580025753.01.T01:CDS | 35.0% |
| !! | GTTCTGGAAGATCAAAAAGA+TGG | - | Chr5:23744696-23744715 | None:intergenic | 35.0% |
| !! | TGTTTTTACCTCAGCTTCAT+GGG | - | Chr5:23745839-23745858 | None:intergenic | 35.0% |
| !!! | GTTTTGTTTGCATCTTCATC+TGG | - | Chr5:23745150-23745169 | None:intergenic | 35.0% |
| AGAGATGCTAAAAGGTGGAA+AGG | - | Chr5:23744978-23744997 | None:intergenic | 40.0% | |
| AGCATCTCTTACTCATCTTG+AGG | + | Chr5:23744989-23745008 | MsG0580025753.01.T01:CDS | 40.0% | |
| GAGATGCTAAAAGGTGGAAA+GGG | - | Chr5:23744977-23744996 | None:intergenic | 40.0% | |
| GAGTAAGAGATGCTAAAAGG+TGG | - | Chr5:23744983-23745002 | None:intergenic | 40.0% | |
| GATCCACAAATTGATCTGTC+TGG | - | Chr5:23745798-23745817 | None:intergenic | 40.0% | |
| TAGCCAGACAGATCAATTTG+TGG | + | Chr5:23745792-23745811 | MsG0580025753.01.T01:CDS | 40.0% | |
| ! | GTTTTTACCTCAGCTTCATG+GGG | - | Chr5:23745838-23745857 | None:intergenic | 40.0% |
| ! | TTTTTACCTCAGCTTCATGG+GGG | - | Chr5:23745837-23745856 | None:intergenic | 40.0% |
| AAACCCTGATATGGCTCACA+AGG | + | Chr5:23745530-23745549 | MsG0580025753.01.T01:CDS | 45.0% | |
| ACCATTGTCAAACTGCTGCA+TGG | - | Chr5:23744888-23744907 | None:intergenic | 45.0% | |
| ATCACCTTTGCACTGAGTTG+GGG | - | Chr5:23745761-23745780 | None:intergenic | 45.0% | |
| ATGCAGCAGTTTGACAATGG+TGG | + | Chr5:23744887-23744906 | MsG0580025753.01.T01:CDS | 45.0% | |
| CATCACCTTTGCACTGAGTT+GGG | - | Chr5:23745762-23745781 | None:intergenic | 45.0% | |
| CTTCCTTGTGAGCCATATCA+GGG | - | Chr5:23745536-23745555 | None:intergenic | 45.0% | |
| GGTGTTCTTTGTCTCTTCTC+TGG | - | Chr5:23745369-23745388 | None:intergenic | 45.0% | |
| TCATCACCTTTGCACTGAGT+TGG | - | Chr5:23745763-23745782 | None:intergenic | 45.0% | |
| TCCATGCAGCAGTTTGACAA+TGG | + | Chr5:23744884-23744903 | MsG0580025753.01.T01:CDS | 45.0% | |
| ! | ATGGTGGTGACAGTAAGATG+TGG | + | Chr5:23744903-23744922 | MsG0580025753.01.T01:CDS | 45.0% |
| GCTTCCTTGTGAGCCATATC+AGG | - | Chr5:23745537-23745556 | None:intergenic | 50.0% | |
| TGTGGATCTTGACTCCCAAG+TGG | + | Chr5:23745810-23745829 | MsG0580025753.01.T01:CDS | 50.0% | |
| ! | TCACCTTTGCACTGAGTTGG+GGG | - | Chr5:23745760-23745779 | None:intergenic | 50.0% |
| AGCTTCATGGGGGTCCACTT+GGG | - | Chr5:23745827-23745846 | None:intergenic | 55.0% | |
| TGTCCCCCAACTCAGTGCAA+AGG | + | Chr5:23745754-23745773 | MsG0580025753.01.T01:CDS | 55.0% | |
| AGTGGACCCCCATGAAGCTG+AGG | + | Chr5:23745828-23745847 | MsG0580025753.01.T01:CDS | 60.0% | |
| CAGCTTCATGGGGGTCCACT+TGG | - | Chr5:23745828-23745847 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 23744679 | 23745861 | 23744679 | ID=MsG0580025753.01;Name=MsG0580025753.01 |
| Chr5 | mRNA | 23744679 | 23745861 | 23744679 | ID=MsG0580025753.01.T01;Parent=MsG0580025753.01;Name=MsG0580025753.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|6|0|195 |
| Chr5 | exon | 23744679 | 23744759 | 23744679 | ID=MsG0580025753.01.T01:exon:4492;Parent=MsG0580025753.01.T01 |
| Chr5 | exon | 23744876 | 23745010 | 23744876 | ID=MsG0580025753.01.T01:exon:4493;Parent=MsG0580025753.01.T01 |
| Chr5 | exon | 23745129 | 23745258 | 23745129 | ID=MsG0580025753.01.T01:exon:4494;Parent=MsG0580025753.01.T01 |
| Chr5 | exon | 23745364 | 23745412 | 23745364 | ID=MsG0580025753.01.T01:exon:4495;Parent=MsG0580025753.01.T01 |
| Chr5 | exon | 23745503 | 23745578 | 23745503 | ID=MsG0580025753.01.T01:exon:4496;Parent=MsG0580025753.01.T01 |
| Chr5 | exon | 23745745 | 23745861 | 23745745 | ID=MsG0580025753.01.T01:exon:4497;Parent=MsG0580025753.01.T01 |
| Chr5 | CDS | 23744679 | 23744759 | 23744679 | ID=MsG0580025753.01.T01:cds;Parent=MsG0580025753.01.T01 |
| Chr5 | CDS | 23744876 | 23745010 | 23744876 | ID=MsG0580025753.01.T01:cds;Parent=MsG0580025753.01.T01 |
| Chr5 | CDS | 23745129 | 23745258 | 23745129 | ID=MsG0580025753.01.T01:cds;Parent=MsG0580025753.01.T01 |
| Chr5 | CDS | 23745364 | 23745412 | 23745364 | ID=MsG0580025753.01.T01:cds;Parent=MsG0580025753.01.T01 |
| Chr5 | CDS | 23745503 | 23745578 | 23745503 | ID=MsG0580025753.01.T01:cds;Parent=MsG0580025753.01.T01 |
| Chr5 | CDS | 23745745 | 23745861 | 23745745 | ID=MsG0580025753.01.T01:cds;Parent=MsG0580025753.01.T01 |
| Gene Sequence |
| Protein sequence |
