AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000784.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGTGTTTATTAAATTTA+AGG 0.093174 1:+11132373 None:intergenic
TTCCACAGGATCATGTTGTT+TGG 0.282843 1:-11132441 MsG0180000784.01.T01:CDS
ACTTGAAAAGCCAAATGTTT+AGG 0.303666 1:+11132504 None:intergenic
TCCACAGGATCATGTTGTTT+GGG 0.305699 1:-11132440 MsG0180000784.01.T01:CDS
ACAATGGAATCCTAAACATT+TGG 0.370419 1:-11132514 MsG0180000784.01.T01:CDS
CTGTGGAAGGAGTGACAAAC+TGG 0.402105 1:+11132456 None:intergenic
TAAATTTAAGGATAATCCTA+TGG 0.446079 1:+11132385 None:intergenic
CAGTTTGTCACTCCTTCCAC+AGG 0.448486 1:-11132455 MsG0180000784.01.T01:CDS
CAAGTGCTCAATGTTCAACC+TGG 0.454270 1:-11132486 MsG0180000784.01.T01:CDS
TGACAAACTGGAACAGTCCC+AGG 0.541759 1:+11132468 None:intergenic
ACACAACAACAGCTTTAACT+CGG 0.579233 1:+11132553 None:intergenic
AAGTGCTCAATGTTCAACCT+GGG 0.607659 1:-11132485 MsG0180000784.01.T01:CDS
ACCCAAACAACATGATCCTG+TGG 0.647573 1:+11132439 None:intergenic
AAACAACATGATCCTGTGGA+AGG 0.648040 1:+11132443 None:intergenic
GTCACACTGATACTTCACAA+TGG 0.703629 1:-11132530 MsG0180000784.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGTGTTTATTAAATTTA+AGG + Chr1:11132560-11132579 None:intergenic 10.0%
!! TAAATTTAAGGATAATCCTA+TGG + Chr1:11132548-11132567 None:intergenic 20.0%
!!! TTGAAAAGTTTTCAATTACT+TGG + Chr1:11132519-11132538 None:intergenic 20.0%
!! AACTTTTCAATTTCATCCAT+AGG - Chr1:11132529-11132548 MsG0180000784.01.T01:CDS 25.0%
ACAATGGAATCCTAAACATT+TGG - Chr1:11132416-11132435 MsG0180000784.01.T01:CDS 30.0%
ACTTGAAAAGCCAAATGTTT+AGG + Chr1:11132429-11132448 None:intergenic 30.0%
! ACACAACAACAGCTTTAACT+CGG + Chr1:11132380-11132399 None:intergenic 35.0%
AAACAACATGATCCTGTGGA+AGG + Chr1:11132490-11132509 None:intergenic 40.0%
AAGTGCTCAATGTTCAACCT+GGG - Chr1:11132445-11132464 MsG0180000784.01.T01:CDS 40.0%
GTCACACTGATACTTCACAA+TGG - Chr1:11132400-11132419 MsG0180000784.01.T01:CDS 40.0%
TTCCACAGGATCATGTTGTT+TGG - Chr1:11132489-11132508 MsG0180000784.01.T01:CDS 40.0%
! TCCACAGGATCATGTTGTTT+GGG - Chr1:11132490-11132509 MsG0180000784.01.T01:CDS 40.0%
ACCCAAACAACATGATCCTG+TGG + Chr1:11132494-11132513 None:intergenic 45.0%
CAAGTGCTCAATGTTCAACC+TGG - Chr1:11132444-11132463 MsG0180000784.01.T01:CDS 45.0%
CAGTTTGTCACTCCTTCCAC+AGG - Chr1:11132475-11132494 MsG0180000784.01.T01:CDS 50.0%
CTGTGGAAGGAGTGACAAAC+TGG + Chr1:11132477-11132496 None:intergenic 50.0%
TGACAAACTGGAACAGTCCC+AGG + Chr1:11132465-11132484 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 11132370 11132582 11132370 ID=MsG0180000784.01;Name=MsG0180000784.01
Chr1 mRNA 11132370 11132582 11132370 ID=MsG0180000784.01.T01;Parent=MsG0180000784.01;Name=MsG0180000784.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|70
Chr1 exon 11132370 11132582 11132370 ID=MsG0180000784.01.T01:exon:1013;Parent=MsG0180000784.01.T01
Chr1 CDS 11132370 11132582 11132370 ID=MsG0180000784.01.T01:cds;Parent=MsG0180000784.01.T01
Gene Sequence

>MsG0180000784.01.T01

ATGGAAGCCGAGTTAAAGCTGTTGTTGTGTGTCACACTGATACTTCACAATGGAATCCTAAACATTTGGCTTTTCAAGTGCTCAATGTTCAACCTGGGACTGTTCCAGTTTGTCACTCCTTCCACAGGATCATGTTGTTTGGGTTTCCAAGTAATTGAAAACTTTTCAATTTCATCCATAGGATTATCCTTAAATTTAATAAACACTATATAG

Protein sequence

>MsG0180000784.01.T01

MEAELKLLLCVTLILHNGILNIWLFKCSMFNLGLFQFVTPSTGSCCLGFQVIENFSISSIGLSLNLINTI*