AlfalfaGEDB | MsG0580027726.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027726.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027726.01.T01	MCI28106.1	55.882	68	29	1	1	68	1	67	9.77E-17	77.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027726.01.T01	A0A392QX48	55.882	68	29	1	1	68	1	67	4.67e-17	77.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047899.01	MsG0580027726.01	0.805570	1.252623e-49	6.649346e-47
MsG0080048773.01	MsG0580027726.01	0.801917	7.181907e-49	3.471644e-46
MsG0080049040.01	MsG0580027726.01	0.807253	5.533461e-50	3.067233e-47
MsG0180000670.01	MsG0580027726.01	0.812497	4.114713e-51	2.620358e-48
MsG0180000782.01	MsG0580027726.01	0.818962	1.490823e-52	1.132299e-49
MsG0180000784.01	MsG0580027726.01	0.856594	2.894045e-62	6.944077e-59
MsG0180000785.01	MsG0580027726.01	0.817502	3.189521e-52	2.326938e-49
MsG0180001085.01	MsG0580027726.01	0.802832	4.652123e-49	2.302011e-46
MsG0180001100.01	MsG0580027726.01	0.818398	2.001090e-52	1.496381e-49
MsG0180001270.01	MsG0580027726.01	0.807432	5.068883e-50	2.822839e-47
MsG0180002929.01	MsG0580027726.01	0.806754	7.054611e-50	3.860140e-47
MsG0180003741.01	MsG0580027726.01	0.813130	2.990681e-51	1.936617e-48
MsG0180004246.01	MsG0580027726.01	0.828468	8.840137e-55	8.794595e-52
MsG0180004735.01	MsG0580027726.01	0.817354	3.444485e-52	2.502611e-49
MsG0180005485.01	MsG0580027726.01	0.804136	2.497276e-49	1.277634e-46
MsG0180005807.01	MsG0580027726.01	0.829788	4.230148e-55	4.374628e-52
MsG0280010569.01	MsG0580027726.01	0.832716	8.063826e-56	9.088591e-53
MsG0480019971.01	MsG0580027726.01	0.816188	6.289325e-52	4.426250e-49
MsG0480020315.01	MsG0580027726.01	0.826251	3.004920e-54	2.803148e-51
MsG0480020690.01	MsG0580027726.01	0.800535	1.377323e-48	6.428802e-46
MsG0480020783.01	MsG0580027726.01	0.811051	8.495407e-51	5.205409e-48
MsG0480020890.01	MsG0580027726.01	0.802906	4.490848e-49	2.226296e-46
MsG0480021060.01	MsG0580027726.01	0.821478	3.951939e-53	3.218724e-50
MsG0480022355.01	MsG0580027726.01	0.819041	1.430156e-52	1.088569e-49
MsG0480022537.01	MsG0580027726.01	0.826808	2.214322e-54	2.098652e-51
MsG0480022624.01	MsG0580027726.01	0.803759	2.990371e-49	1.515210e-46
MsG0480022647.01	MsG0580027726.01	0.810905	9.136882e-51	5.576952e-48
MsG0480023565.01	MsG0580027726.01	0.804651	1.949878e-49	1.010910e-46
MsG0480023992.01	MsG0580027726.01	0.822027	2.950054e-53	2.440293e-50
MsG0580024873.01	MsG0580027726.01	0.800385	1.477569e-48	6.870793e-46
MsG0580025607.01	MsG0580027726.01	0.804845	1.776573e-49	9.257323e-47
MsG0580025668.01	MsG0580027726.01	0.818046	2.404099e-52	1.780139e-49
MsG0580025753.01	MsG0580027726.01	0.818443	1.954662e-52	1.463518e-49
MsG0580025828.01	MsG0580027726.01	0.808134	3.596112e-50	2.039772e-47
MsG0580025906.01	MsG0580027726.01	0.808087	3.679907e-50	2.084721e-47
MsG0380015438.01	MsG0580027726.01	0.801546	8.557094e-49	4.097616e-46
MsG0380015645.01	MsG0580027726.01	0.811550	6.619246e-51	4.109524e-48
MsG0380015896.01	MsG0580027726.01	0.810191	1.303142e-50	7.803835e-48
MsG0380016284.01	MsG0580027726.01	0.807647	4.564794e-50	2.556241e-47
MsG0380016864.01	MsG0580027726.01	0.811806	5.822595e-51	3.639339e-48
MsG0380017087.01	MsG0580027726.01	0.835360	1.756393e-56	2.142944e-53
MsG0380018069.01	MsG0580027726.01	0.810090	1.370434e-50	8.185410e-48
MsG0480018200.01	MsG0580027726.01	0.808367	3.207167e-50	1.830464e-47
MsG0480018423.01	MsG0580027726.01	0.814650	1.383051e-51	9.333426e-49
MsG0580027394.01	MsG0580027726.01	0.801296	9.626913e-49	4.581128e-46
MsG0580027655.01	MsG0580027726.01	0.867500	1.307790e-65	4.576004e-62
MsG0580027725.01	MsG0580027726.01	0.824529	7.681305e-54	6.821984e-51
MsG0580027726.01	MsG0580027763.01	0.812544	4.018859e-51	2.562554e-48
MsG0580027726.01	MsG0580027826.01	0.812424	4.269041e-51	2.713506e-48
MsG0580027726.01	MsG0580028723.01	0.820188	7.828316e-53	6.151616e-50
MsG0580027726.01	MsG0580029414.01	0.830738	2.480429e-55	2.636472e-52
MsG0580027726.01	MsG0580029529.01	0.814164	1.770645e-51	1.179287e-48
MsG0580027726.01	MsG0680030680.01	0.814793	1.285903e-51	8.711156e-49
MsG0580027726.01	MsG0680030784.01	0.832077	1.161157e-55	1.284093e-52
MsG0580027726.01	MsG0680030895.01	0.833899	4.091567e-56	4.777982e-53
MsG0580027726.01	MsG0680030908.01	0.846905	1.619486e-59	2.829539e-56
MsG0580027726.01	MsG0680031469.01	0.806018	1.008634e-49	5.416357e-47
MsG0580027726.01	MsG0680031621.01	0.808414	3.134153e-50	1.790903e-47
MsG0580027726.01	MsG0680032830.01	0.804735	1.873135e-49	9.732967e-47
MsG0580027726.01	MsG0680034064.01	0.800826	1.200882e-48	5.646917e-46
MsG0580027726.01	MsG0680034767.01	0.820143	8.016483e-53	6.291859e-50
MsG0580027726.01	MsG0680035307.01	0.821207	4.565592e-53	3.690773e-50
MsG0580027726.01	MsG0780036243.01	0.846892	1.632211e-59	2.850572e-56
MsG0580027726.01	MsG0780036517.01	0.801308	9.573738e-49	4.557116e-46
MsG0580027726.01	MsG0780036593.01	0.807428	5.078147e-50	2.827776e-47
MsG0580027726.01	MsG0780036596.01	0.818074	2.369798e-52	1.756093e-49
MsG0580027726.01	MsG0780036979.01	0.814985	1.165755e-51	7.938382e-49
MsG0580027726.01	MsG0780037341.01	0.815483	9.031961e-52	6.235314e-49
MsG0580027726.01	MsG0780037395.01	0.800053	1.726292e-48	7.959875e-46
MsG0580027726.01	MsG0780037399.01	0.803731	3.030859e-49	1.534595e-46
MsG0580027726.01	MsG0780039940.01	0.805437	1.335428e-49	7.064927e-47
MsG0580027726.01	MsG0780039975.01	0.823494	1.344049e-53	1.159139e-50
MsG0580027726.01	MsG0780040278.01	0.807847	4.139349e-50	2.330152e-47
MsG0580027726.01	MsG0780040430.01	0.826048	3.358646e-54	3.115104e-51
MsG0580027726.01	MsG0780040431.01	0.833897	4.096676e-56	4.783540e-53
MsG0580027726.01	MsG0780041791.01	0.809629	1.721773e-50	1.016136e-47
MsG0580027726.01	MsG0880041834.01	0.812778	3.572645e-51	2.291849e-48
MsG0580027726.01	MsG0880042958.01	0.801297	9.624672e-49	4.580116e-46
MsG0580027726.01	MsG0880044487.01	0.809903	1.502869e-50	8.933239e-48
MsG0580027726.01	MsG0880044538.01	0.810545	1.093112e-50	6.607624e-48
MsG0580027726.01	MsG0880044704.01	0.814360	1.602849e-51	1.073206e-48
MsG0580027726.01	MsG0880045558.01	0.820840	5.544353e-53	4.436403e-50
MsG0580027726.01	MsG0880045668.01	0.835341	1.775387e-56	2.164949e-53
MsG0580027726.01	MsG0880047394.01	0.810063	1.388742e-50	8.289366e-48
MsG0280006592.01	MsG0580027726.01	0.808687	2.740525e-50	1.577109e-47
MsG0280006937.01	MsG0580027726.01	0.817537	3.131845e-52	2.286982e-49
MsG0280007750.01	MsG0580027726.01	0.835145	1.989590e-56	2.411584e-53
MsG0280008390.01	MsG0580027726.01	0.801780	7.660720e-49	3.690187e-46
MsG0280008418.01	MsG0580027726.01	0.822197	2.694241e-53	2.239524e-50
MsG0280008899.01	MsG0580027726.01	0.823118	1.644779e-53	1.403566e-50
MsG0280009635.01	MsG0580027726.01	0.838776	2.351542e-57	3.182354e-54
MsG0280009756.01	MsG0580027726.01	0.803570	3.273281e-49	1.650595e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027726.01.T01	MTR_2g068840	55.072	69	30	1	1	69	296	363	9.01e-18	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027726.01.T01	AT3G12900	43.077	65	36	1	2	66	292	355	1.60e-11	57.8

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTATGTAGACAACTTCTT+TGG	0.237823	5:+67476672	MsG0580027726.01.T01:CDS
ATCTTTCTTTCCACCAATTC+TGG	0.291514	5:-67476616	None:intergenic
GATTGGCCCGTTACCAGAAT+TGG	0.402403	5:+67476603	MsG0580027726.01.T01:CDS
TTCTTTGGGAGTTCTCATAA+AGG	0.412724	5:+67476687	MsG0580027726.01.T01:CDS
ATTATGTAGACAACTTCTTT+GGG	0.417144	5:+67476673	MsG0580027726.01.T01:CDS
TTTCCACCAATTCTGGTAAC+GGG	0.440919	5:-67476609	None:intergenic
CTTTCCACCAATTCTGGTAA+CGG	0.463161	5:-67476610	None:intergenic
AGAATTGGTGGAAAGAAAGA+TGG	0.504086	5:+67476618	MsG0580027726.01.T01:CDS
TGGCCCGTTACCAGAATTGG+TGG	0.534702	5:+67476606	MsG0580027726.01.T01:CDS
CCTATGCCAACAGAGATGAT+TGG	0.551257	5:+67476586	MsG0580027726.01.T01:CDS
CCAATCATCTCTGTTGGCAT+AGG	0.556190	5:-67476586	None:intergenic
AACGGGCCAATCATCTCTGT+TGG	0.572721	5:-67476592	None:intergenic
TGGTGGAAAGAAAGATGGAT+TGG	0.602584	5:+67476623	MsG0580027726.01.T01:CDS
ATTGATACTCTGGGATTGAG+TGG	0.613546	5:-67476547	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAATGGAATTGATACTCT+GGG	-	Chr5:67476559-67476578	None:intergenic	25.0%
!	ATTATGTAGACAACTTCTTT+GGG	+	Chr5:67476673-67476692	MsG0580027726.01.T01:CDS	25.0%
	GAAAAATGGAATTGATACTC+TGG	-	Chr5:67476560-67476579	None:intergenic	30.0%
	GATTATGTAGACAACTTCTT+TGG	+	Chr5:67476672-67476691	MsG0580027726.01.T01:CDS	30.0%
!	CTGAATATAGAGAAGTTTTG+TGG	+	Chr5:67476646-67476665	MsG0580027726.01.T01:CDS	30.0%
	AGAATTGGTGGAAAGAAAGA+TGG	+	Chr5:67476618-67476637	MsG0580027726.01.T01:CDS	35.0%
	ATCTTTCTTTCCACCAATTC+TGG	-	Chr5:67476619-67476638	None:intergenic	35.0%
!	GATTTTGCAAAGATCAACTC+TGG	+	Chr5:67476723-67476742	MsG0580027726.01.T01:CDS	35.0%
!	TTCTTTGGGAGTTCTCATAA+AGG	+	Chr5:67476687-67476706	MsG0580027726.01.T01:CDS	35.0%
!!	ATATAGAGAAGTTTTGTGGC+AGG	+	Chr5:67476650-67476669	MsG0580027726.01.T01:CDS	35.0%
	CTTTCCACCAATTCTGGTAA+CGG	-	Chr5:67476613-67476632	None:intergenic	40.0%
	GGCATAGGATGAGTGAAAAA+TGG	-	Chr5:67476574-67476593	None:intergenic	40.0%
	TGGTGGAAAGAAAGATGGAT+TGG	+	Chr5:67476623-67476642	MsG0580027726.01.T01:CDS	40.0%
	TTTCCACCAATTCTGGTAAC+GGG	-	Chr5:67476612-67476631	None:intergenic	40.0%
!!	ATTGATACTCTGGGATTGAG+TGG	-	Chr5:67476550-67476569	None:intergenic	40.0%
	CCAATCATCTCTGTTGGCAT+AGG	-	Chr5:67476589-67476608	None:intergenic	45.0%
	CCTATGCCAACAGAGATGAT+TGG	+	Chr5:67476586-67476605	MsG0580027726.01.T01:CDS	45.0%
	AACGGGCCAATCATCTCTGT+TGG	-	Chr5:67476595-67476614	None:intergenic	50.0%
!	GATTGGCCCGTTACCAGAAT+TGG	+	Chr5:67476603-67476622	MsG0580027726.01.T01:CDS	50.0%
	TGGCCCGTTACCAGAATTGG+TGG	+	Chr5:67476606-67476625	MsG0580027726.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	67476537	67476749	67476537	ID=MsG0580027726.01;Name=MsG0580027726.01
Chr5	mRNA	67476537	67476749	67476537	ID=MsG0580027726.01.T01;Parent=MsG0580027726.01;Name=MsG0580027726.01.T01;_AED=0.49;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|70
Chr5	exon	67476537	67476749	67476537	ID=MsG0580027726.01.T01:exon:44169;Parent=MsG0580027726.01.T01
Chr5	CDS	67476537	67476749	67476537	ID=MsG0580027726.01.T01:cds;Parent=MsG0580027726.01.T01

Gene Sequence	>MsG0580027726.01.T01 ATGACAACATCCACTCAATCCCAGAGTATCAATTCCATTTTTCACTCATCCTATGCCAACAGAGATGATTGGCCCGTTACCAGAATTGGTGGAAAGAAAGATGGATTGGCTGAATATAGAGAAGTTTTGTGGCAGGATTATGTAGACAACTTCTTTGGGAGTTCTCATAAAGGAAAGAAGACTCTTGATTTTGCAAAGATCAACTCTGGCTAA
Protein sequence	>MsG0580027726.01.T01 MTTSTQSQSINSIFHSSYANRDDWPVTRIGGKKDGLAEYREVLWQDYVDNFFGSSHKGKKTLDFAKINSG*