AlfalfaGEDB | MsG0580028409.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028409.01

KEGG: K02942
GO: -
PFAM: PF00428

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028409.01.T01	PNX74316.1	91.045	67	6	0	1	67	1	67	2.48E-34	125

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028409.01.T01	P52855	62.5	64	24	0	1	64	1	64	1.90E-22	88.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028409.01.T01	A0A2K3L706	91.045	67	6	0	1	67	1	67	1.19e-34	125

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0380016607.01	MsG0580028409.01	PPI
MsG0380016607.01	MsG0580028409.01	PPI
MsG0380016653.01	MsG0580028409.01	PPI
MsG0780036015.01	MsG0580028409.01	PPI
MsG0580028409.01	MsG0380016653.01	PPI
MsG0280010495.01	MsG0580028409.01	PPI
MsG0780036073.01	MsG0580028409.01	PPI
MsG0280010002.01	MsG0580028409.01	PPI
MsG0280006717.01	MsG0580028409.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028409.01.T01	MTR_3g092600	93.443	61	4	0	1	61	1	61	2.33e-35	118
MsG0580028409.01.T01	MTR_2g066750	93.443	61	4	0	1	61	1	61	2.33e-35	118
MsG0580028409.01.T01	MTR_8g024350	63.889	72	25	1	1	72	1	71	1.00e-25	94.4
MsG0580028409.01.T01	MTR_7g118060	61.017	59	23	0	1	59	1	59	3.26e-19	77.8
MsG0580028409.01.T01	MTR_7g005740	61.290	62	5	1	1	62	1	43	6.71e-16	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028409.01.T01	AT5G24510	54.545	88	37	1	1	85	1	88	1.96e-26	96.3
MsG0580028409.01.T01	AT5G47700	63.158	57	21	0	5	61	6	62	1.58e-20	81.3
MsG0580028409.01.T01	AT5G47700	63.158	57	21	0	5	61	6	62	1.58e-20	81.3
MsG0580028409.01.T01	AT1G01100	61.404	57	22	0	5	61	6	62	2.79e-20	80.1
MsG0580028409.01.T01	AT1G01100	61.404	57	22	0	5	61	6	62	4.50e-20	80.1
MsG0580028409.01.T01	AT1G01100	61.404	57	22	0	5	61	6	62	4.50e-20	80.1
MsG0580028409.01.T01	AT1G01100	61.404	57	22	0	5	61	6	62	4.50e-20	80.1
MsG0580028409.01.T01	AT4G00810	59.649	57	23	0	5	61	6	62	2.41e-19	78.2
MsG0580028409.01.T01	AT4G00810	59.649	57	23	0	5	61	6	62	2.41e-19	78.2

Find 45 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCGTATTTGCACCTGCTTC+TGG	0.236382	5:-80183563	MsG0580028409.01.T01:CDS
AGATAGTTGATTGCAAGTTT+TGG	0.308451	5:+80183385	None:intergenic
GTCATCGTCACTTTCTTCCT+TGG	0.329724	5:+80183491	None:intergenic
ACGTCACCGTTGAATCTTAC+TGG	0.348075	5:-80183670	MsG0580028409.01.T01:CDS
GATAGCAGACTTACGGTGAT+AGG	0.389185	5:+80183807	None:intergenic
ATTGTTCTGCCACTCAAACT+AGG	0.408152	5:+80183903	None:intergenic
CCAGACTAGGACTCCTTTCA+AGG	0.415086	5:-80183420	MsG0580028409.01.T01:CDS
CCTTGAAAGGAGTCCTAGTC+TGG	0.418177	5:+80183420	None:intergenic
CAAAATGAGTTCCAACGAAT+CGG	0.420231	5:-80183871	MsG0580028409.01.T01:exon
TTTGCACCTGCTTCTGGAGG+TGG	0.421586	5:-80183557	MsG0580028409.01.T01:CDS
TCTGAGCAAGCTTAGCGAAT+AGG	0.441352	5:+80183642	None:intergenic
TCCAGAAGCAGGTGCAAATA+CGG	0.450862	5:+80183562	None:intergenic
ACCTTGATCCTCCACGATGA+TGG	0.469006	5:-80183834	MsG0580028409.01.T01:CDS
AACTGCAGCCGTTGAAAAGA+AGG	0.478736	5:-80183520	MsG0580028409.01.T01:CDS
GAAGATCGGTACTTTGTTGA+AGG	0.484661	5:-80183699	MsG0580028409.01.T01:CDS
AAAATGAGTTCCAACGAATC+GGG	0.488892	5:-80183870	MsG0580028409.01.T01:CDS
TTGTTCTGCCACTCAAACTA+GGG	0.493480	5:+80183904	None:intergenic
ACCGTTGAATCTTACTGGCC+AGG	0.494210	5:-80183665	MsG0580028409.01.T01:CDS
GCGGCTCCACCTCCAGAAGC+AGG	0.495490	5:+80183551	None:intergenic
TGATGATGTGTTGTTGATGA+AGG	0.502176	5:-80183284	MsG0580028409.01.T01:three_prime_UTR
CGGCTGCAGTTCGGTTGTTG+CGG	0.504668	5:+80183532	None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	5:-80183596	MsG0580028409.01.T01:CDS
TCCATCATCGTGGAGGATCA+AGG	0.519306	5:+80183833	None:intergenic
TAGTTTGTTGTAGCCAGACT+AGG	0.521961	5:-80183433	MsG0580028409.01.T01:CDS
GGAAGAAAGTGACGATGACA+TGG	0.523361	5:-80183487	MsG0580028409.01.T01:CDS
AGATTCAACGGTGACGTTAG+CGG	0.526016	5:+80183676	None:intergenic
GCAAGCTTAGCGAATAGGCC+TGG	0.531593	5:+80183647	None:intergenic
TTTGTTTAGGCGAAGAAGAT+CGG	0.532771	5:-80183713	MsG0580028409.01.T01:intron
ATAGCAGACTTACGGTGATA+GGG	0.561399	5:+80183808	None:intergenic
TGAAAAGAAGGAGGAAGCCA+AGG	0.574510	5:-80183508	MsG0580028409.01.T01:CDS
GTAAATGCAGCCCGATTCGT+TGG	0.581909	5:+80183860	None:intergenic
CAGACTAGGACTCCTTTCAA+GGG	0.583004	5:-80183419	MsG0580028409.01.T01:CDS
GCCTGGCCAGTAAGATTCAA+CGG	0.587082	5:+80183664	None:intergenic
TTCGTAAACCCTAGTTTGAG+TGG	0.588932	5:-80183912	MsG0580028409.01.T01:five_prime_UTR
GGTGGCACCACCAACAGCGC+CGG	0.589428	5:+80183589	None:intergenic
GTATTTGCACCTGCTTCTGG+AGG	0.615296	5:-80183560	MsG0580028409.01.T01:CDS
AGCAGGTGCAAATACGGTAA+CGG	0.621107	5:+80183568	None:intergenic
AGACTAGGACTCCTTTCAAG+GGG	0.655500	5:-80183418	MsG0580028409.01.T01:CDS
TGCAGCCGTTGAAAAGAAGG+AGG	0.676132	5:-80183517	MsG0580028409.01.T01:CDS
GAAGAAAGTGACGATGACAT+GGG	0.678372	5:-80183486	MsG0580028409.01.T01:CDS
TGATAGGGATTCCATCATCG+TGG	0.699207	5:+80183823	None:intergenic
TAGGGATTCCATCATCGTGG+AGG	0.711417	5:+80183826	None:intergenic
GACTAGGACTCCTTTCAAGG+GGG	0.714058	5:-80183417	MsG0580028409.01.T01:CDS
AGGTGCAAATACGGTAACGG+TGG	0.719409	5:+80183571	None:intergenic
ATCATCGTGGAGGATCAAGG+TGG	0.755949	5:+80183836	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAACTCAAAAACAACTA+TGG	+	Chr5:80183918-80183937	None:intergenic	20.0%
!!	AAAAACTCAAAAACAACTAT+GGG	+	Chr5:80183917-80183936	None:intergenic	20.0%
!!	TACAGAAATAGAATTGAATA+GGG	+	Chr5:80183384-80183403	None:intergenic	20.0%
!!	TTACAGAAATAGAATTGAAT+AGG	+	Chr5:80183385-80183404	None:intergenic	20.0%
!!!	ATTTTTCTTTGTTACTGTTT+GGG	-	Chr5:80183428-80183447	MsG0580028409.01.T01:CDS	20.0%
!!!	GTTGTTTTTGAGTTTTTTAT+TGG	-	Chr5:80183920-80183939	MsG0580028409.01.T01:five_prime_UTR	20.0%
!!	CATTTTTCTTTGTTACTGTT+TGG	-	Chr5:80183427-80183446	MsG0580028409.01.T01:CDS	25.0%
!	AGATAGTTGATTGCAAGTTT+TGG	+	Chr5:80183784-80183803	None:intergenic	30.0%
!!	TACTGTTTGGGTTTTTGTTT+AGG	-	Chr5:80183440-80183459	MsG0580028409.01.T01:CDS	30.0%
	AAAATGAGTTCCAACGAATC+GGG	-	Chr5:80183296-80183315	MsG0580028409.01.T01:three_prime_UTR	35.0%
	AATGAGATCTATGAGCGATT+TGG	+	Chr5:80183411-80183430	None:intergenic	35.0%
	CAAAATGAGTTCCAACGAAT+CGG	-	Chr5:80183295-80183314	MsG0580028409.01.T01:three_prime_UTR	35.0%
	TTTGTTTAGGCGAAGAAGAT+CGG	-	Chr5:80183453-80183472	MsG0580028409.01.T01:CDS	35.0%
!	TGATGATGTGTTGTTGATGA+AGG	-	Chr5:80183882-80183901	MsG0580028409.01.T01:exon	35.0%
	ATAGCAGACTTACGGTGATA+GGG	+	Chr5:80183361-80183380	None:intergenic	40.0%
	ATTGTTCTGCCACTCAAACT+AGG	+	Chr5:80183266-80183285	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	Chr5:80183680-80183699	MsG0580028409.01.T01:CDS	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	Chr5:80183467-80183486	MsG0580028409.01.T01:CDS	40.0%
	GAATAGGGATAGCAGACTTA+CGG	+	Chr5:80183369-80183388	None:intergenic	40.0%
	TTCGTAAACCCTAGTTTGAG+TGG	-	Chr5:80183254-80183273	MsG0580028409.01.T01:three_prime_UTR	40.0%
	TTGTTCTGCCACTCAAACTA+GGG	+	Chr5:80183265-80183284	None:intergenic	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	Chr5:80183558-80183577	MsG0580028409.01.T01:CDS	40.0%
!!	TAGTTTGTTGTAGCCAGACT+AGG	-	Chr5:80183733-80183752	MsG0580028409.01.T01:intron	40.0%
	AACTGCAGCCGTTGAAAAGA+AGG	-	Chr5:80183646-80183665	MsG0580028409.01.T01:CDS	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	Chr5:80183496-80183515	MsG0580028409.01.T01:CDS	45.0%
	AGACTAGGACTCCTTTCAAG+GGG	-	Chr5:80183748-80183767	MsG0580028409.01.T01:intron	45.0%
	AGATTCAACGGTGACGTTAG+CGG	+	Chr5:80183493-80183512	None:intergenic	45.0%
	AGCAGGTGCAAATACGGTAA+CGG	+	Chr5:80183601-80183620	None:intergenic	45.0%
	CAGACTAGGACTCCTTTCAA+GGG	-	Chr5:80183747-80183766	MsG0580028409.01.T01:intron	45.0%
	GATAGCAGACTTACGGTGAT+AGG	+	Chr5:80183362-80183381	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	Chr5:80183679-80183698	MsG0580028409.01.T01:CDS	45.0%
	TCCAGAAGCAGGTGCAAATA+CGG	+	Chr5:80183607-80183626	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	Chr5:80183527-80183546	None:intergenic	45.0%
	TGAAAAGAAGGAGGAAGCCA+AGG	-	Chr5:80183658-80183677	MsG0580028409.01.T01:CDS	45.0%
	TGATAGGGATTCCATCATCG+TGG	+	Chr5:80183346-80183365	None:intergenic	45.0%
!	GGCTTCCTCCTTCTTTTCAA+CGG	+	Chr5:80183657-80183676	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	Chr5:80183678-80183697	None:intergenic	45.0%
!	TCTTTTCAACGGCTGCAGTT+CGG	+	Chr5:80183646-80183665	None:intergenic	45.0%
	ACCGTATTTGCACCTGCTTC+TGG	-	Chr5:80183603-80183622	MsG0580028409.01.T01:CDS	50.0%
	ACCGTTGAATCTTACTGGCC+AGG	-	Chr5:80183501-80183520	MsG0580028409.01.T01:CDS	50.0%
	ACCTTGATCCTCCACGATGA+TGG	-	Chr5:80183332-80183351	MsG0580028409.01.T01:three_prime_UTR	50.0%
	ATCATCGTGGAGGATCAAGG+TGG	+	Chr5:80183333-80183352	None:intergenic	50.0%
	CCAGACTAGGACTCCTTTCA+AGG	-	Chr5:80183746-80183765	MsG0580028409.01.T01:intron	50.0%
	CCTTGAAAGGAGTCCTAGTC+TGG	+	Chr5:80183749-80183768	None:intergenic	50.0%
	GACTAGGACTCCTTTCAAGG+GGG	-	Chr5:80183749-80183768	MsG0580028409.01.T01:intron	50.0%
	GCCTGGCCAGTAAGATTCAA+CGG	+	Chr5:80183505-80183524	None:intergenic	50.0%
	GTATTTGCACCTGCTTCTGG+AGG	-	Chr5:80183606-80183625	MsG0580028409.01.T01:CDS	50.0%
	TAGGGATTCCATCATCGTGG+AGG	+	Chr5:80183343-80183362	None:intergenic	50.0%
	TCCATCATCGTGGAGGATCA+AGG	+	Chr5:80183336-80183355	None:intergenic	50.0%
	TGCAGCCGTTGAAAAGAAGG+AGG	-	Chr5:80183649-80183668	MsG0580028409.01.T01:CDS	50.0%
!	AGGTGCAAATACGGTAACGG+TGG	+	Chr5:80183598-80183617	None:intergenic	50.0%
!	GTAAATGCAGCCCGATTCGT+TGG	+	Chr5:80183309-80183328	None:intergenic	50.0%
	GATTCAGCCACCCCCTTGAA+AGG	+	Chr5:80183762-80183781	None:intergenic	55.0%
	GCAAGCTTAGCGAATAGGCC+TGG	+	Chr5:80183522-80183541	None:intergenic	55.0%
	TAGGACTCCTTTCAAGGGGG+TGG	-	Chr5:80183752-80183771	MsG0580028409.01.T01:intron	55.0%
!	TTTGCACCTGCTTCTGGAGG+TGG	-	Chr5:80183609-80183628	MsG0580028409.01.T01:CDS	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	Chr5:80183567-80183586	MsG0580028409.01.T01:CDS	55.0%
	CGGCTGCAGTTCGGTTGTTG+CGG	+	Chr5:80183637-80183656	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	Chr5:80183570-80183589	MsG0580028409.01.T01:CDS	60.0%
	GCGGCTCCACCTCCAGAAGC+AGG	+	Chr5:80183618-80183637	None:intergenic	70.0%
	GGTGGCACCACCAACAGCGC+CGG	+	Chr5:80183580-80183599	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	80183249	80183939	80183249	ID=MsG0580028409.01;Name=MsG0580028409.01
Chr5	mRNA	80183249	80183939	80183249	ID=MsG0580028409.01.T01;Parent=MsG0580028409.01;Name=MsG0580028409.01.T01;_AED=0.02;_eAED=0.02;_QI=50\|1\|1\|1\|1\|1\|2\|115\|143
Chr5	exon	80183249	80183726	80183249	ID=MsG0580028409.01.T01:exon:7629;Parent=MsG0580028409.01.T01
Chr5	exon	80183821	80183939	80183821	ID=MsG0580028409.01.T01:exon:7628;Parent=MsG0580028409.01.T01
Chr5	five_prime_UTR	80183890	80183939	80183890	ID=MsG0580028409.01.T01:five_prime_utr;Parent=MsG0580028409.01.T01
Chr5	CDS	80183821	80183889	80183821	ID=MsG0580028409.01.T01:cds;Parent=MsG0580028409.01.T01
Chr5	CDS	80183364	80183726	80183364	ID=MsG0580028409.01.T01:cds;Parent=MsG0580028409.01.T01
Chr5	three_prime_UTR	80183249	80183363	80183249	ID=MsG0580028409.01.T01:three_prime_utr;Parent=MsG0580028409.01.T01

Gene Sequence

>MsG0580028409.01.T01

ATGAGTTCCAACGAATCGGGCTGCATTTACGCCACCTTGATCCTCCACGATGATGGAATCCCTATCACCGCGAAGAAGATCGGTACTTTGTTGAAGGCCGCTAACGTCACCGTTGAATCTTACTGGCCAGGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTCGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCACCGTTACCGTATTTGCACCTGCTTCTGGAGGTGGAGCCGCAACAACCGAACTGCAGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAAGTTTCTCTGTATAGTTTGTTGTAGCCAGACTAGGACTCCTTTCAAGGGGGTGGCTGAATCCAAAACTTGCAATCAACTATCTCTTTATAAATTGTTTATCTAA

Protein sequence

>MsG0580028409.01.T01

MSSNESGCIYATLILHDDGIPITAKKIGTLLKAANVTVESYWPGLFAKLAQSKNVDDLVLNSGAVGGATVTVFAPASGGGAATTELQPLKRRRKPRKKVTMTWALVCLIKFLCIVCCSQTRTPFKGVAESKTCNQLSLYKLFI*