Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036073.01.T01 | AES77205.1 | 65.854 | 205 | 28 | 5 | 1 | 182 | 1 | 186 | 1.77E-61 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036073.01.T01 | P52855 | 67.188 | 64 | 21 | 0 | 1 | 64 | 1 | 64 | 1.32E-24 | 95.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036073.01.T01 | G7KWM5 | 65.854 | 205 | 28 | 5 | 1 | 182 | 1 | 186 | 8.45e-62 | 199 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036073.01.T01 | MTR_7g005740 | 65.854 | 205 | 28 | 5 | 1 | 182 | 1 | 186 | 2.14e-65 | 199 |
| MsG0780036073.01.T01 | MTR_3g092600 | 95.082 | 61 | 3 | 0 | 1 | 61 | 1 | 61 | 3.43e-36 | 122 |
| MsG0780036073.01.T01 | MTR_2g066750 | 95.082 | 61 | 3 | 0 | 1 | 61 | 1 | 61 | 3.43e-36 | 122 |
| MsG0780036073.01.T01 | MTR_8g024350 | 67.606 | 71 | 22 | 1 | 1 | 71 | 1 | 70 | 1.46e-26 | 97.8 |
| MsG0780036073.01.T01 | MTR_7g118060 | 64.407 | 59 | 21 | 0 | 1 | 59 | 1 | 59 | 1.08e-18 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036073.01.T01 | AT5G24510 | 57.143 | 112 | 45 | 3 | 1 | 111 | 1 | 110 | 1.27e-32 | 113 |
| MsG0780036073.01.T01 | AT5G47700 | 47.748 | 111 | 55 | 2 | 2 | 112 | 3 | 110 | 2.26e-22 | 87.0 |
| MsG0780036073.01.T01 | AT5G47700 | 47.748 | 111 | 55 | 2 | 2 | 112 | 3 | 110 | 2.26e-22 | 87.0 |
| MsG0780036073.01.T01 | AT1G01100 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 1.00e-21 | 85.1 |
| MsG0780036073.01.T01 | AT1G01100 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 1.59e-21 | 85.1 |
| MsG0780036073.01.T01 | AT1G01100 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 1.59e-21 | 85.1 |
| MsG0780036073.01.T01 | AT1G01100 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 1.59e-21 | 85.1 |
| MsG0780036073.01.T01 | AT4G00810 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.08e-20 | 82.8 |
| MsG0780036073.01.T01 | AT4G00810 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.08e-20 | 82.8 |
Find 34 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTCTATAATCAACTTCT+AGG | 0.201812 | 7:+2085800 | None:intergenic |
| ATGTCGTGCAAAGTATATTT+TGG | 0.240282 | 7:+2085943 | None:intergenic |
| GATAATAGACTTACGGTGAT+TGG | 0.316212 | 7:+2086750 | None:intergenic |
| GATGATGATATAGGTTGTTT+TGG | 0.338031 | 7:-2086003 | MsG0780036073.01.T01:CDS |
| TCATCTGAGCTCTCGACTTC+AGG | 0.351887 | 7:+2086021 | None:intergenic |
| TCTGAGCAAGCTTAGCGAAT+AGG | 0.392998 | 7:+2086605 | None:intergenic |
| AAAATGAGTTCCGGCGAGTT+GGG | 0.395726 | 7:-2086813 | MsG0780036073.01.T01:CDS |
| ATCTTTGATAGTCGTACTAA+TGG | 0.401580 | 7:-2085922 | MsG0780036073.01.T01:CDS |
| ACCTAGAAGTTGATTATAGA+AGG | 0.404052 | 7:-2085801 | MsG0780036073.01.T01:CDS |
| GATGAGAATGGCAGAAAATT+CGG | 0.405059 | 7:-2085829 | MsG0780036073.01.T01:CDS |
| GAAGATCGGCACTTTGTTGA+AGG | 0.430819 | 7:-2086662 | MsG0780036073.01.T01:CDS |
| CAAAATGAGTTCCGGCGAGT+TGG | 0.444981 | 7:-2086814 | None:intergenic |
| ATAATAGACTTACGGTGATT+GGG | 0.445449 | 7:+2086751 | None:intergenic |
| TTTGATGACTTTGATTGCAT+TGG | 0.451838 | 7:-2085874 | MsG0780036073.01.T01:CDS |
| TTGATGACTTTGATTGCATT+GGG | 0.462464 | 7:-2085873 | MsG0780036073.01.T01:CDS |
| GAAGGCATTATGTGCGTTAG+TGG | 0.466016 | 7:-2085783 | None:intergenic |
| TTGAACTCCGGCTCTGTTGG+TGG | 0.474989 | 7:-2086559 | MsG0780036073.01.T01:CDS |
| GAGAGCTCAGATGATGATAT+AGG | 0.478337 | 7:-2086012 | MsG0780036073.01.T01:CDS |
| ACGTCACCGTTGAATTCTAC+TGG | 0.509674 | 7:-2086633 | MsG0780036073.01.T01:CDS |
| GGCGGCACCACCAACAGAGC+CGG | 0.512281 | 7:+2086552 | None:intergenic |
| AACTCAGACGTTGATGAGAA+TGG | 0.522044 | 7:-2085841 | MsG0780036073.01.T01:CDS |
| ACAATTCACAAAATGAGTTC+CGG | 0.522809 | 7:-2086822 | None:intergenic |
| TCCATCGTCGTGGAGGATCA+AGG | 0.532980 | 7:+2086776 | None:intergenic |
| GCAAGCTTAGCGAATAGGCT+TGG | 0.542190 | 7:+2086610 | None:intergenic |
| GGCTGCGGGACAAGTGAGGA+AGG | 0.571317 | 7:-2085982 | MsG0780036073.01.T01:CDS |
| ACCTTGATCCTCCACGACGA+TGG | 0.573503 | 7:-2086777 | MsG0780036073.01.T01:CDS |
| GGAAACGAATCAGCATGAGC+AGG | 0.589625 | 7:+2086520 | None:intergenic |
| GTAAATGCAACCCAACTCGC+CGG | 0.617289 | 7:+2086803 | None:intergenic |
| ATGAGCAGGTTCAGCAACGG+CGG | 0.622677 | 7:+2086534 | None:intergenic |
| AGCATGAGCAGGTTCAGCAA+CGG | 0.627947 | 7:+2086531 | None:intergenic |
| TTGGGATTCCATCGTCGTGG+AGG | 0.650788 | 7:+2086769 | None:intergenic |
| TGATTGGGATTCCATCGTCG+TGG | 0.662981 | 7:+2086766 | None:intergenic |
| GCTTGGCCAGTAGAATTCAA+CGG | 0.676718 | 7:+2086627 | None:intergenic |
| ATCGTCGTGGAGGATCAAGG+TGG | 0.743990 | 7:+2086779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAAATAAAAAGTACAAT+TGG | + | Chr7:2086381-2086400 | None:intergenic | 10.0% |
| !!! | TAGATCTCATTTTTTTTTTT+AGG | - | Chr7:2085907-2085926 | MsG0780036073.01.T01:CDS | 15.0% |
| !! | GAAAAAAGATAATAGACTTA+CGG | + | Chr7:2085856-2085875 | None:intergenic | 20.0% |
| !! | TGAAAAAGAAATAACTGAAA+AGG | + | Chr7:2086131-2086150 | None:intergenic | 20.0% |
| !!! | AATTGGTTTATAAAATGAGA+AGG | + | Chr7:2086364-2086383 | None:intergenic | 20.0% |
| !!! | ATTGGTTTATAAAATGAGAA+GGG | + | Chr7:2086363-2086382 | None:intergenic | 20.0% |
| !!! | GAAATTATTTGAAACTTGTT+GGG | - | Chr7:2086187-2086206 | MsG0780036073.01.T01:intron | 20.0% |
| !!! | TGAAATTATTTGAAACTTGT+TGG | - | Chr7:2086186-2086205 | MsG0780036073.01.T01:intron | 20.0% |
| ! | AATGTGTCAAAACAATAATC+AGG | - | Chr7:2086223-2086242 | MsG0780036073.01.T01:intron | 25.0% |
| ! | ATTAAAATATACTCACCCTT+GGG | - | Chr7:2086491-2086510 | MsG0780036073.01.T01:intron | 25.0% |
| ! | TTGGTTTATAAAATGAGAAG+GGG | + | Chr7:2086362-2086381 | None:intergenic | 25.0% |
| ACAAAAGACAAGTTGCTAAA+CGG | - | Chr7:2086249-2086268 | MsG0780036073.01.T01:intron | 30.0% | |
| ATAATAGACTTACGGTGATT+GGG | + | Chr7:2085848-2085867 | None:intergenic | 30.0% | |
| GATTAAAATATACTCACCCT+TGG | - | Chr7:2086490-2086509 | MsG0780036073.01.T01:intron | 30.0% | |
| TCAAAGCATACATATGATCT+GGG | - | Chr7:2086284-2086303 | MsG0780036073.01.T01:intron | 30.0% | |
| TGATCAATTAAGAAACCCAA+GGG | + | Chr7:2086509-2086528 | None:intergenic | 30.0% | |
| TGGTTTATAAAATGAGAAGG+GGG | + | Chr7:2086361-2086380 | None:intergenic | 30.0% | |
| TTGATCAATTAAGAAACCCA+AGG | + | Chr7:2086510-2086529 | None:intergenic | 30.0% | |
| ! | ACCTAGAAGTTGATTATAGA+AGG | - | Chr7:2086795-2086814 | MsG0780036073.01.T01:CDS | 30.0% |
| ! | ATACATATGATCTGGGATAT+CGG | - | Chr7:2086291-2086310 | MsG0780036073.01.T01:intron | 30.0% |
| ! | ATCTTTGATAGTCGTACTAA+TGG | - | Chr7:2086674-2086693 | MsG0780036073.01.T01:intron | 30.0% |
| ! | ATGTCGTGCAAAGTATATTT+TGG | + | Chr7:2086656-2086675 | None:intergenic | 30.0% |
| ! | GATGATGATATAGGTTGTTT+TGG | - | Chr7:2086593-2086612 | MsG0780036073.01.T01:CDS | 30.0% |
| ! | TTGATGACTTTGATTGCATT+GGG | - | Chr7:2086723-2086742 | MsG0780036073.01.T01:intron | 30.0% |
| ! | TTTGATGACTTTGATTGCAT+TGG | - | Chr7:2086722-2086741 | MsG0780036073.01.T01:intron | 30.0% |
| !!! | AGCAACTTCTTTTTTGTCTT+CGG | + | Chr7:2086552-2086571 | None:intergenic | 30.0% |
| !!! | TTTTTTTAGGCAGAGAAGAT+CGG | - | Chr7:2085920-2085939 | MsG0780036073.01.T01:CDS | 30.0% |
| AATGAGATCTATGAGCGATT+TGG | + | Chr7:2085898-2085917 | None:intergenic | 35.0% | |
| CTCAAAGCATACATATGATC+TGG | - | Chr7:2086283-2086302 | MsG0780036073.01.T01:intron | 35.0% | |
| GAAATAACTGAAAAGGAACG+AGG | + | Chr7:2086124-2086143 | None:intergenic | 35.0% | |
| GATAATAGACTTACGGTGAT+TGG | + | Chr7:2085849-2085868 | None:intergenic | 35.0% | |
| GATGAGAATGGCAGAAAATT+CGG | - | Chr7:2086767-2086786 | MsG0780036073.01.T01:CDS | 35.0% | |
| GCCTTCTATAATCAACTTCT+AGG | + | Chr7:2086799-2086818 | None:intergenic | 35.0% | |
| ! | GATGATCTCATTTTGAACTC+CGG | - | Chr7:2086025-2086044 | MsG0780036073.01.T01:CDS | 35.0% |
| !!! | GCAACTTCTTTTTTGTCTTC+GGG | + | Chr7:2086551-2086570 | None:intergenic | 35.0% |
| GAAGAAGAAGAAGAAGACGA+AGG | + | Chr7:2086100-2086119 | None:intergenic | 40.0% | |
| GAGAGCTCAGATGATGATAT+AGG | - | Chr7:2086584-2086603 | MsG0780036073.01.T01:CDS | 40.0% | |
| ! | AACTCAGACGTTGATGAGAA+TGG | - | Chr7:2086755-2086774 | MsG0780036073.01.T01:intron | 40.0% |
| !!! | ATATAGGTTGTTTTGGCTGC+GGG | - | Chr7:2086600-2086619 | MsG0780036073.01.T01:CDS | 40.0% |
| !!! | GATATAGGTTGTTTTGGCTG+CGG | - | Chr7:2086599-2086618 | MsG0780036073.01.T01:CDS | 40.0% |
| ACAAGTTGCTAAACGGAGAG+AGG | - | Chr7:2086256-2086275 | MsG0780036073.01.T01:intron | 45.0% | |
| ACGTCACCGTTGAATTCTAC+TGG | - | Chr7:2085963-2085982 | MsG0780036073.01.T01:CDS | 45.0% | |
| GAAGATCGGCACTTTGTTGA+AGG | - | Chr7:2085934-2085953 | MsG0780036073.01.T01:CDS | 45.0% | |
| GCTTGGCCAGTAGAATTCAA+CGG | + | Chr7:2085972-2085991 | None:intergenic | 45.0% | |
| TCTGAGCAAGCTTAGCGAAT+AGG | + | Chr7:2085994-2086013 | None:intergenic | 45.0% | |
| ! | ATCTCACAAGCGTCGCAAAT+GGG | - | Chr7:2086323-2086342 | MsG0780036073.01.T01:intron | 45.0% |
| ! | ATTTTGAACTCCGGCTCTGT+TGG | - | Chr7:2086034-2086053 | MsG0780036073.01.T01:CDS | 45.0% |
| ! | TATCTCACAAGCGTCGCAAA+TGG | - | Chr7:2086322-2086341 | MsG0780036073.01.T01:intron | 45.0% |
| AGCATGAGCAGGTTCAGCAA+CGG | + | Chr7:2086068-2086087 | None:intergenic | 50.0% | |
| GCAAGCTTAGCGAATAGGCT+TGG | + | Chr7:2085989-2086008 | None:intergenic | 50.0% | |
| GGAAACGAATCAGCATGAGC+AGG | + | Chr7:2086079-2086098 | None:intergenic | 50.0% | |
| GTAAATGCAACCCAACTCGC+CGG | + | Chr7:2085796-2085815 | None:intergenic | 50.0% | |
| TCATCTGAGCTCTCGACTTC+AGG | + | Chr7:2086578-2086597 | None:intergenic | 50.0% | |
| TGATTGGGATTCCATCGTCG+TGG | + | Chr7:2085833-2085852 | None:intergenic | 50.0% | |
| ACCTTGATCCTCCACGACGA+TGG | - | Chr7:2085819-2085838 | MsG0780036073.01.T01:CDS | 55.0% | |
| ATCGTCGTGGAGGATCAAGG+TGG | + | Chr7:2085820-2085839 | None:intergenic | 55.0% | |
| ATGAGCAGGTTCAGCAACGG+CGG | + | Chr7:2086065-2086084 | None:intergenic | 55.0% | |
| TCCATCGTCGTGGAGGATCA+AGG | + | Chr7:2085823-2085842 | None:intergenic | 55.0% | |
| TTGAACTCCGGCTCTGTTGG+TGG | - | Chr7:2086037-2086056 | MsG0780036073.01.T01:CDS | 55.0% | |
| TTGGGATTCCATCGTCGTGG+AGG | + | Chr7:2085830-2085849 | None:intergenic | 55.0% | |
| !! | TTTTGGCTGCGGGACAAGTG+AGG | - | Chr7:2086610-2086629 | MsG0780036073.01.T01:CDS | 55.0% |
| GGCTGCGGGACAAGTGAGGA+AGG | - | Chr7:2086614-2086633 | MsG0780036073.01.T01:CDS | 65.0% | |
| GGCGGCACCACCAACAGAGC+CGG | + | Chr7:2086047-2086066 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 2085786 | 2086832 | 2085786 | ID=MsG0780036073.01;Name=MsG0780036073.01 |
| Chr7 | mRNA | 2085786 | 2086832 | 2085786 | ID=MsG0780036073.01.T01;Parent=MsG0780036073.01;Name=MsG0780036073.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|183 |
| Chr7 | exon | 2086764 | 2086832 | 2086764 | ID=MsG0780036073.01.T01:exon:38486;Parent=MsG0780036073.01.T01 |
| Chr7 | exon | 2086494 | 2086689 | 2086494 | ID=MsG0780036073.01.T01:exon:38485;Parent=MsG0780036073.01.T01 |
| Chr7 | exon | 2085786 | 2086072 | 2085786 | ID=MsG0780036073.01.T01:exon:38484;Parent=MsG0780036073.01.T01 |
| Chr7 | CDS | 2086764 | 2086832 | 2086764 | ID=MsG0780036073.01.T01:cds;Parent=MsG0780036073.01.T01 |
| Chr7 | CDS | 2086494 | 2086689 | 2086494 | ID=MsG0780036073.01.T01:cds;Parent=MsG0780036073.01.T01 |
| Chr7 | CDS | 2085786 | 2086072 | 2085786 | ID=MsG0780036073.01.T01:cds;Parent=MsG0780036073.01.T01 |
| Gene Sequence |
| Protein sequence |
