AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016653.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016653.01.T01 MTR_3g092600 99.091 110 1 0 1 110 1 110 3.25e-74 215
MsG0380016653.01.T01 MTR_2g066750 98.182 110 2 0 1 110 1 110 1.11e-73 214
MsG0380016653.01.T01 MTR_8g024350 64.545 110 37 2 1 110 1 108 1.48e-33 112
MsG0380016653.01.T01 MTR_7g118060 62.712 59 22 0 1 59 1 59 1.04e-20 80.5
MsG0380016653.01.T01 MTR_7g005740 66.129 62 2 1 1 62 1 43 1.57e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016653.01.T01 AT5G24510 57.391 115 40 4 1 110 1 111 4.31e-36 119
MsG0380016653.01.T01 AT5G47700 57.658 111 45 1 2 110 3 113 8.29e-29 100
MsG0380016653.01.T01 AT5G47700 57.658 111 45 1 2 110 3 113 8.29e-29 100
MsG0380016653.01.T01 AT1G01100 60.185 108 42 1 4 110 5 112 4.43e-28 99.0
MsG0380016653.01.T01 AT1G01100 60.185 108 42 1 4 110 5 112 4.43e-28 99.0
MsG0380016653.01.T01 AT1G01100 60.185 108 42 1 4 110 5 112 4.43e-28 99.0
MsG0380016653.01.T01 AT1G01100 50.459 109 39 1 2 110 3 96 2.22e-27 96.7
MsG0380016653.01.T01 AT4G00810 54.955 111 48 1 2 110 3 113 2.98e-27 97.1
MsG0380016653.01.T01 AT4G00810 54.955 111 48 1 2 110 3 113 2.98e-27 97.1

Find 44 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAAAACAAAACAAGTTT+TGG 0.254235 3:+86165418 None:intergenic
TTCCCGCATCAGATGTAAAA+TGG 0.280734 3:+86165282 None:intergenic
CAAAATGAGTTCCGGCGAAT+TGG 0.294649 3:-86166834 MsG0380016653.01.T01:exon
GTCATCGTCACTTTCTTCCT+TGG 0.329724 3:+86165523 None:intergenic
ACGTCACCGTTGAATCTTAC+TGG 0.348075 3:-86166628 MsG0380016653.01.T01:CDS
AAAATGAGTTCCGGCGAATT+GGG 0.368280 3:-86166833 MsG0380016653.01.T01:CDS
TAGTTTGTTGTAGCCAGATT+AGG 0.371827 3:-86165465 MsG0380016653.01.T01:three_prime_UTR
CCTTGAAAGGAGTCCTAATC+TGG 0.373967 3:+86165452 None:intergenic
CCAGATTAGGACTCCTTTCA+AGG 0.391998 3:-86165452 MsG0380016653.01.T01:three_prime_UTR
TCTGAGCAAGCTTAGCGAAT+AGG 0.392998 3:+86166600 None:intergenic
GATAATAGACTTACGGTGAT+TGG 0.399296 3:+86166770 None:intergenic
GCCGTATCTGCACCCGCTGC+TGG 0.401410 3:-86166521 MsG0380016653.01.T01:CDS
TCTGCACCCGCTGCTGGTGG+TGG 0.439771 3:-86166515 MsG0380016653.01.T01:CDS
ATTGTTCTGCCACACAAACT+AGG 0.448236 3:+86166866 None:intergenic
GCGGCTCCACCACCAGCAGC+GGG 0.451994 3:+86166509 None:intergenic
ATAATAGACTTACGGTGATT+GGG 0.452174 3:+86166771 None:intergenic
GAAGATCGGTACTTTGTTGA+AGG 0.461021 3:-86166657 MsG0380016653.01.T01:CDS
ACCTGCCGCCGTTGAAAAGA+AGG 0.476631 3:-86166474 MsG0380016653.01.T01:intron
TGCGGCTCCACCACCAGCAG+CGG 0.489051 3:+86166508 None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG 0.510539 3:-86166554 MsG0380016653.01.T01:CDS
ACAATTCACAAAATGAGTTC+CGG 0.522809 3:-86166842 MsG0380016653.01.T01:exon
GGAAGAAAGTGACGATGACA+TGG 0.523361 3:-86165519 MsG0380016653.01.T01:CDS
AGCGGCACCACCAACAGCGC+CGG 0.527844 3:+86166547 None:intergenic
ACCAGCAGCGGGTGCAGATA+CGG 0.530473 3:+86166520 None:intergenic
GGATTCAGCCACTCCTTGAA+AGG 0.532068 3:+86165439 None:intergenic
TCCATCGTCGTGGAGGATCA+AGG 0.532980 3:+86166796 None:intergenic
GCAAGCTTAGCGAATAGGCT+TGG 0.542190 3:+86166605 None:intergenic
TTTGTTTAGGCGGAGAAGAT+CGG 0.543289 3:-86166671 MsG0380016653.01.T01:intron
GGCAGGTTCGGCAGCTGCTG+CGG 0.548908 3:+86166490 None:intergenic
TTGTTCTGCCACACAAACTA+GGG 0.550348 3:+86166867 None:intergenic
GTAAATGCAGCCCAATTCGC+CGG 0.571217 3:+86166823 None:intergenic
ACCTTGATCCTCCACGACGA+TGG 0.573503 3:-86166797 MsG0380016653.01.T01:CDS
TTAGGACTCCTTTCAAGGAG+TGG 0.585485 3:-86165447 MsG0380016653.01.T01:three_prime_UTR
TTCGTAAACCCTAGTTTGTG+TGG 0.589308 3:-86166875 MsG0380016653.01.T01:five_prime_UTR
AAAATCTTTCAAAATTAACC+TGG 0.590654 3:+86165250 None:intergenic
GTATCTGCACCCGCTGCTGG+TGG 0.603091 3:-86166518 MsG0380016653.01.T01:CDS
ATGCGGGAATATATCATACC+AGG 0.604298 3:-86165268 MsG0380016653.01.T01:three_prime_UTR
GCTTGGCCAGTAAGATTCAA+CGG 0.629260 3:+86166622 None:intergenic
TTGGGATTCCATCGTCGTGG+AGG 0.650788 3:+86166789 None:intergenic
GTGTTTGTAGGAGGAAGCCA+AGG 0.654949 3:-86165540 MsG0380016653.01.T01:intron
TGATTGGGATTCCATCGTCG+TGG 0.662981 3:+86166786 None:intergenic
GGGTGCAGATACGGCAACAG+CGG 0.665524 3:+86166529 None:intergenic
GAAGAAAGTGACGATGACAT+GGG 0.678372 3:-86165518 MsG0380016653.01.T01:CDS
ATCGTCGTGGAGGATCAAGG+TGG 0.743990 3:+86166799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATATGTTTTTATCTTA+AGG + Chr3:86165867-86165886 None:intergenic 10.0%
!! AATTGTATTGAAATTAGAAA+CGG - Chr3:86165768-86165787 MsG0380016653.01.T01:intron 15.0%
!! TATAAAAGATATAATTTCCT+CGG - Chr3:86165990-86166009 MsG0380016653.01.T01:intron 15.0%
!!! CTCATTTTATTGTTAAATTT+TGG - Chr3:86166782-86166801 MsG0380016653.01.T01:intron 15.0%
!!! TTATAAAACAAAACAAGTTT+TGG + Chr3:86166727-86166746 None:intergenic 15.0%
!!! TTTTCATGCAAAATAATTTT+TGG + Chr3:86166061-86166080 None:intergenic 15.0%
!! AAAGTAAAAAATGTGGATAT+AGG + Chr3:86166430-86166449 None:intergenic 20.0%
!! AAGTAAAAAATGTGGATATA+GGG + Chr3:86166429-86166448 None:intergenic 20.0%
!! ATTGTATTGAAATTAGAAAC+GGG - Chr3:86165769-86165788 MsG0380016653.01.T01:intron 20.0%
!!! AATTGTTGTTTTTCTTCATT+GGG - Chr3:86166140-86166159 MsG0380016653.01.T01:intron 20.0%
!!! ATATCCACATTTTTTACTTT+TGG - Chr3:86166430-86166449 MsG0380016653.01.T01:intron 20.0%
!!! ATGAAAACATGTAGTTATTT+TGG - Chr3:86166098-86166117 MsG0380016653.01.T01:intron 20.0%
!!! ATTTTTCTTTTGTTACTGTT+TGG - Chr3:86165441-86165460 MsG0380016653.01.T01:three_prime_UTR 20.0%
!!! TAATTGTTGTTTTTCTTCAT+TGG - Chr3:86166139-86166158 MsG0380016653.01.T01:intron 20.0%
!!! TATCCACATTTTTTACTTTT+GGG - Chr3:86166431-86166450 MsG0380016653.01.T01:intron 20.0%
!!! TTTTTCTTTTGTTACTGTTT+GGG - Chr3:86165442-86165461 MsG0380016653.01.T01:three_prime_UTR 20.0%
! CGATTTGAAGATTATAGAAA+TGG + Chr3:86165409-86165428 None:intergenic 25.0%
! GACAACTCTGAATATAATTA+TGG - Chr3:86166190-86166209 MsG0380016653.01.T01:intron 25.0%
! TATAGAAATGGAATTGAAGA+GGG + Chr3:86165397-86165416 None:intergenic 25.0%
! TTAAATAAGCCAATCATAAC+TGG - Chr3:86166403-86166422 MsG0380016653.01.T01:intron 25.0%
! TTATAGAAATGGAATTGAAG+AGG + Chr3:86165398-86165417 None:intergenic 25.0%
!! AAACAATACCTTCTTTTCAA+CGG + Chr3:86165679-86165698 None:intergenic 25.0%
!!! ATAAAGTTAACTTGTTTTCC+TGG - Chr3:86166320-86166339 MsG0380016653.01.T01:intron 25.0%
!!! TAAAGTTAACTTGTTTTCCT+GGG - Chr3:86166321-86166340 MsG0380016653.01.T01:intron 25.0%
AAAAGATATAATTTCCTCGG+AGG - Chr3:86165993-86166012 MsG0380016653.01.T01:intron 30.0%
AAAGATATAATTTCCTCGGA+GGG - Chr3:86165994-86166013 MsG0380016653.01.T01:intron 30.0%
AATGTAAGCTTATGCATAAG+TGG + Chr3:86166275-86166294 None:intergenic 30.0%
ACAATTCACAAAATGAGTTC+CGG - Chr3:86165300-86165319 MsG0380016653.01.T01:three_prime_UTR 30.0%
AGATGCAAGTTCTACAAAAT+AGG + Chr3:86165967-86165986 None:intergenic 30.0%
ATAATAGACTTACGGTGATT+GGG + Chr3:86165374-86165393 None:intergenic 30.0%
ATGTAAGCTTATGCATAAGT+GGG + Chr3:86166274-86166293 None:intergenic 30.0%
CAGCAAATGACAGTATTTAT+CGG + Chr3:86166030-86166049 None:intergenic 30.0%
TATTCAGAGTTGTCTTGAAA+AGG + Chr3:86166184-86166203 None:intergenic 30.0%
TCACCCAAAAGTAAAAAATG+TGG + Chr3:86166437-86166456 None:intergenic 30.0%
TGTAATGATGAGTTACTGAT+TGG - Chr3:86165739-86165758 MsG0380016653.01.T01:intron 30.0%
TGTTAAATGAATGCTAGTTC+TGG - Chr3:86165816-86165835 MsG0380016653.01.T01:intron 30.0%
TTTGTTTAGTGACACTGTTT+GGG + Chr3:86166376-86166395 None:intergenic 30.0%
! ATTCCATTTTACATCTGATG+CGG - Chr3:86166857-86166876 MsG0380016653.01.T01:five_prime_UTR 30.0%
! ATTTTGTAGAACTTGCATCT+AGG - Chr3:86165967-86165986 MsG0380016653.01.T01:intron 30.0%
AAGATATAATTTCCTCGGAG+GGG - Chr3:86165995-86166014 MsG0380016653.01.T01:intron 35.0%
CTTCATTGGGATTTGAGAAT+TGG - Chr3:86166153-86166172 MsG0380016653.01.T01:intron 35.0%
GAAGAGGGATAATAGACTTA+CGG + Chr3:86165382-86165401 None:intergenic 35.0%
GATAATAGACTTACGGTGAT+TGG + Chr3:86165375-86165394 None:intergenic 35.0%
GTTTGTTTAGTGACACTGTT+TGG + Chr3:86166377-86166396 None:intergenic 35.0%
TCATTCCATACTGTGTTTGT+AGG - Chr3:86166590-86166609 MsG0380016653.01.T01:CDS 35.0%
! ATTTTGTGCTGTGATCAAGT+TGG - Chr3:86165934-86165953 MsG0380016653.01.T01:intron 35.0%
! TCTTTTGTTACTGTTTGGGT+TGG - Chr3:86165446-86165465 MsG0380016653.01.T01:three_prime_UTR 35.0%
! TTCCATTTTACATCTGATGC+GGG - Chr3:86166858-86166877 MsG0380016653.01.T01:five_prime_UTR 35.0%
! TTTTGTGCTGTGATCAAGTT+GGG - Chr3:86165935-86165954 MsG0380016653.01.T01:intron 35.0%
!! TAGTTTGTTGTAGCCAGATT+AGG - Chr3:86166677-86166696 MsG0380016653.01.T01:intron 35.0%
!!! GTTTGGGTTGGTTTTTGTTT+AGG - Chr3:86165458-86165477 MsG0380016653.01.T01:three_prime_UTR 35.0%
AAAATGAGTTCCGGCGAATT+GGG - Chr3:86165309-86165328 MsG0380016653.01.T01:three_prime_UTR 40.0%
ATCAACAAGACAATCTTCCC+AGG + Chr3:86166341-86166360 None:intergenic 40.0%
ATGCGGGAATATATCATACC+AGG - Chr3:86166874-86166893 MsG0380016653.01.T01:five_prime_UTR 40.0%
ATTGTTCTGCCACACAAACT+AGG + Chr3:86165279-86165298 None:intergenic 40.0%
GAAGAAAGTGACGATGACAT+GGG - Chr3:86166624-86166643 MsG0380016653.01.T01:CDS 40.0%
GAAGATCGGTACTTTGTTGA+AGG - Chr3:86165485-86165504 MsG0380016653.01.T01:exon 40.0%
GATATAGGGCCAGTTATGAT+TGG + Chr3:86166415-86166434 None:intergenic 40.0%
TTCCATACTGTGTTTGTAGG+AGG - Chr3:86166593-86166612 MsG0380016653.01.T01:CDS 40.0%
TTCCCGCATCAGATGTAAAA+TGG + Chr3:86166863-86166882 None:intergenic 40.0%
TTCCTCCTACAAACACAGTA+TGG + Chr3:86166598-86166617 None:intergenic 40.0%
TTGTTCTGCCACACAAACTA+GGG + Chr3:86165278-86165297 None:intergenic 40.0%
TTTGTTTAGGCGGAGAAGAT+CGG - Chr3:86165471-86165490 MsG0380016653.01.T01:three_prime_UTR 40.0%
! CAATACCTTCTTTTCAACGG+CGG + Chr3:86165676-86165695 None:intergenic 40.0%
! TTCGTAAACCCTAGTTTGTG+TGG - Chr3:86165267-86165286 MsG0380016653.01.T01:three_prime_UTR 40.0%
! TTTGTGCTGTGATCAAGTTG+GGG - Chr3:86165936-86165955 MsG0380016653.01.T01:intron 40.0%
!! GATGATCTCGTTTTGAACTC+CGG - Chr3:86165576-86165595 MsG0380016653.01.T01:intron 40.0%
!!! TGGGTTGGTTTTTGTTTAGG+CGG - Chr3:86165461-86165480 MsG0380016653.01.T01:three_prime_UTR 40.0%
ACGTCACCGTTGAATCTTAC+TGG - Chr3:86165514-86165533 MsG0380016653.01.T01:CDS 45.0%
CAAAATGAGTTCCGGCGAAT+TGG - Chr3:86165308-86165327 MsG0380016653.01.T01:three_prime_UTR 45.0%
CCAGATTAGGACTCCTTTCA+AGG - Chr3:86166690-86166709 MsG0380016653.01.T01:intron 45.0%
CCTTGAAAGGAGTCCTAATC+TGG + Chr3:86166693-86166712 None:intergenic 45.0%
GCTTGGCCAGTAAGATTCAA+CGG + Chr3:86165523-86165542 None:intergenic 45.0%
GGAAGAAAGTGACGATGACA+TGG - Chr3:86166623-86166642 MsG0380016653.01.T01:CDS 45.0%
TCTGAGCAAGCTTAGCGAAT+AGG + Chr3:86165545-86165564 None:intergenic 45.0%
TTAGGACTCCTTTCAAGGAG+TGG - Chr3:86166695-86166714 MsG0380016653.01.T01:intron 45.0%
! GTCATCGTCACTTTCTTCCT+TGG + Chr3:86166622-86166641 None:intergenic 45.0%
GCAAGCTTAGCGAATAGGCT+TGG + Chr3:86165540-86165559 None:intergenic 50.0%
GGATTCAGCCACTCCTTGAA+AGG + Chr3:86166706-86166725 None:intergenic 50.0%
GTAAATGCAGCCCAATTCGC+CGG + Chr3:86165322-86165341 None:intergenic 50.0%
GTGTTTGTAGGAGGAAGCCA+AGG - Chr3:86166602-86166621 MsG0380016653.01.T01:CDS 50.0%
TGATTGGGATTCCATCGTCG+TGG + Chr3:86165359-86165378 None:intergenic 50.0%
ACCTGCCGCCGTTGAAAAGA+AGG - Chr3:86165668-86165687 MsG0380016653.01.T01:intron 55.0%
ACCTTGATCCTCCACGACGA+TGG - Chr3:86165345-86165364 MsG0380016653.01.T01:three_prime_UTR 55.0%
ATCGTCGTGGAGGATCAAGG+TGG + Chr3:86165346-86165365 None:intergenic 55.0%
TCCATCGTCGTGGAGGATCA+AGG + Chr3:86165349-86165368 None:intergenic 55.0%
TTGGGATTCCATCGTCGTGG+AGG + Chr3:86165356-86165375 None:intergenic 55.0%
! ACCTTCTTTTCAACGGCGGC+AGG + Chr3:86165672-86165691 None:intergenic 55.0%
! CTTTTCAACGGCGGCAGGTT+CGG + Chr3:86165667-86165686 None:intergenic 55.0%
!! GTTTTGAACTCCGGCGCTGT+TGG - Chr3:86165585-86165604 MsG0380016653.01.T01:intron 55.0%
ACCAGCAGCGGGTGCAGATA+CGG + Chr3:86165625-86165644 None:intergenic 60.0%
CGGATACAGAATCCCCTCCG+AGG + Chr3:86166010-86166029 None:intergenic 60.0%
GGGTGCAGATACGGCAACAG+CGG + Chr3:86165616-86165635 None:intergenic 60.0%
TTGAACTCCGGCGCTGTTGG+TGG - Chr3:86165588-86165607 MsG0380016653.01.T01:intron 60.0%
GTATCTGCACCCGCTGCTGG+TGG - Chr3:86165624-86165643 MsG0380016653.01.T01:intron 65.0%
AGCGGCACCACCAACAGCGC+CGG + Chr3:86165598-86165617 None:intergenic 70.0%
GCCGTATCTGCACCCGCTGC+TGG - Chr3:86165621-86165640 MsG0380016653.01.T01:intron 70.0%
GGCAGGTTCGGCAGCTGCTG+CGG + Chr3:86165655-86165674 None:intergenic 70.0%
TCTGCACCCGCTGCTGGTGG+TGG - Chr3:86165627-86165646 MsG0380016653.01.T01:intron 70.0%
TGCGGCTCCACCACCAGCAG+CGG + Chr3:86165637-86165656 None:intergenic 70.0%
GCGGCTCCACCACCAGCAGC+GGG + Chr3:86165636-86165655 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 86165261 86166903 86165261 ID=MsG0380016653.01;Name=MsG0380016653.01
Chr3 mRNA 86165261 86166903 86165261 ID=MsG0380016653.01.T01;Parent=MsG0380016653.01;Name=MsG0380016653.01.T01;_AED=0.47;_eAED=0.47;_QI=51|1|1|1|0.5|0.66|3|238|110
Chr3 exon 86165261 86165552 86165261 ID=MsG0380016653.01.T01:exon:22112;Parent=MsG0380016653.01.T01
Chr3 exon 86166475 86166684 86166475 ID=MsG0380016653.01.T01:exon:22111;Parent=MsG0380016653.01.T01
Chr3 exon 86166784 86166903 86166784 ID=MsG0380016653.01.T01:exon:22110;Parent=MsG0380016653.01.T01
Chr3 five_prime_UTR 86166853 86166903 86166853 ID=MsG0380016653.01.T01:five_prime_utr;Parent=MsG0380016653.01.T01
Chr3 CDS 86166784 86166852 86166784 ID=MsG0380016653.01.T01:cds;Parent=MsG0380016653.01.T01
Chr3 CDS 86166475 86166684 86166475 ID=MsG0380016653.01.T01:cds;Parent=MsG0380016653.01.T01
Chr3 CDS 86165499 86165552 86165499 ID=MsG0380016653.01.T01:cds;Parent=MsG0380016653.01.T01
Chr3 three_prime_UTR 86165261 86165498 86165261 ID=MsG0380016653.01.T01:three_prime_utr;Parent=MsG0380016653.01.T01
Gene Sequence

>MsG0380016653.01.T01

ATGAGTTCCGGCGAATTGGGCTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCAATCACCGCGGAGAAGATCGGTACTTTGTTGAAGGCTGCTAACGTCACCGTTGAATCTTACTGGCCAAGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCGCTGTTGCCGTATCTGCACCCGCTGCTGGTGGTGGAGCCGCAGCAGCTGCCGAACCTGCCGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAA

Protein sequence

>MsG0380016653.01.T01

MSSGELGCIYATLILHDDGIPITAEKIGTLLKAANVTVESYWPSLFAKLAQSKNVDDLVLNSGAVGGAAVAVSAPAAGGGAAAAAEPAAVEKKEEAKEESDDDMGFSLFD*