AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029101.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029101.01.T01 MTR_7g062720 65.909 132 45 0 1 132 1 132 9.98e-60 190
MsG0580029101.01.T01 MTR_5g061150 62.992 127 47 0 20 146 10 136 2.18e-48 160
MsG0580029101.01.T01 MTR_4g037355 57.971 138 35 5 151 287 1 116 2.52e-41 142
MsG0580029101.01.T01 MTR_4g112730 71.831 71 18 1 138 208 1 69 1.03e-26 102
MsG0580029101.01.T01 MTR_1g036690 58.537 82 27 3 280 360 5 80 1.06e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 5:-91971180 MsG0580029101.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 5:+91971128 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 5:-91970285 MsG0580029101.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 5:-91970293 MsG0580029101.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 5:-91970905 MsG0580029101.01.T01:CDS
TATGGAAGGGGACGTGGTTT+TGG 0.259444 5:-91970520 MsG0580029101.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 5:+91970982 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 5:-91971166 MsG0580029101.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 5:-91970990 MsG0580029101.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 5:+91970616 None:intergenic
TATAGGTGGGGTTCTATTCC+TGG 0.331534 5:+91970131 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 5:+91970316 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 5:-91970233 MsG0580029101.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 5:-91971161 MsG0580029101.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.353242 5:-91971160 MsG0580029101.01.T01:CDS
CGTGGACGTGGTCGTGGTTA+TGG 0.362984 5:-91970538 MsG0580029101.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 5:+91970922 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 5:-91970241 MsG0580029101.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 5:-91970972 MsG0580029101.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 5:-91970989 MsG0580029101.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 5:-91970364 MsG0580029101.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 5:+91970617 None:intergenic
CGTGGTTATGGAAGGGGACG+TGG 0.429618 5:-91970526 MsG0580029101.01.T01:CDS
CCTTCGTCACCACCTTCATA+AGG 0.433246 5:-91971037 MsG0580029101.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 5:-91970830 MsG0580029101.01.T01:CDS
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 5:-91970580 MsG0580029101.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 5:-91970574 MsG0580029101.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 5:-91971076 MsG0580029101.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG 0.460742 5:+91971110 None:intergenic
AGAAATCAGCAGCATCTAAG+TGG 0.464577 5:+91970203 None:intergenic
TTTCACGGTATTGTTGCTGC+AGG 0.465441 5:+91970749 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 5:-91970415 MsG0580029101.01.T01:CDS
GCAGCAACAATACCGTGAAA+AGG 0.474876 5:-91970746 MsG0580029101.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG 0.475930 5:-91970652 MsG0580029101.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.483138 5:+91970622 None:intergenic
ACTTCAAAATAAGGATAAAA+GGG 0.487291 5:-91970284 MsG0580029101.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 5:-91971120 MsG0580029101.01.T01:CDS
CAGCAACAATACCGTGAAAA+GGG 0.498612 5:-91970745 MsG0580029101.01.T01:CDS
GACGTGGTCGTGGTTATGGA+AGG 0.501950 5:-91970534 MsG0580029101.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 5:-91970604 MsG0580029101.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 5:-91970357 MsG0580029101.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 5:-91970568 MsG0580029101.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 5:-91970924 MsG0580029101.01.T01:CDS
TACATTAGATGCATGAAAAG+TGG 0.516304 5:+91970774 None:intergenic
CATGGTCGTGGACGTGGACG+TGG 0.524744 5:-91970556 MsG0580029101.01.T01:CDS
CGTGGACGTGGACGTGGTCG+TGG 0.524981 5:-91970544 MsG0580029101.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 5:-91970149 MsG0580029101.01.T01:CDS
CATGGAGATACAATTAAAGA+AGG 0.533844 5:-91971102 MsG0580029101.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 5:-91971126 MsG0580029101.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 5:-91970562 MsG0580029101.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 5:-91970550 MsG0580029101.01.T01:CDS
CGTGGTCGTGGTTATGGAAG+GGG 0.551769 5:-91970532 MsG0580029101.01.T01:CDS
CTTAATATCATGCCTGCTTG+TGG 0.567363 5:-91970704 MsG0580029101.01.T01:CDS
TATGGACAAAATCGTGGTCG+TGG 0.573891 5:-91970586 MsG0580029101.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 5:-91970875 MsG0580029101.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 5:-91971070 MsG0580029101.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 5:-91970373 MsG0580029101.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 5:+91970926 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 5:+91971037 None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG 0.598966 5:-91970533 MsG0580029101.01.T01:CDS
AGTATTTGCTATCGTTGTGG+AGG 0.599695 5:-91970370 MsG0580029101.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 5:+91970204 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 5:-91971125 MsG0580029101.01.T01:CDS
CACGTCCACGACCATGACCA+CGG 0.661673 5:+91970563 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 5:+91971047 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 5:-91970838 MsG0580029101.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 5:+91970636 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 5:+91970637 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 5:-91970592 MsG0580029101.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr5:91970933-91970952 MsG0580029101.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr5:91971064-91971083 MsG0580029101.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG - Chr5:91970182-91970201 MsG0580029101.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG - Chr5:91970222-91970241 MsG0580029101.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG - Chr5:91970223-91970242 MsG0580029101.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr5:91971063-91971082 MsG0580029101.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr5:91971107-91971126 MsG0580029101.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr5:91971115-91971134 MsG0580029101.01.T01:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr5:91971035-91971054 None:intergenic 25.0%
!! TATTTTCATCTCTAATGAGA+TGG + Chr5:91971196-91971215 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr5:91970923-91970942 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG + Chr5:91970223-91970242 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG - Chr5:91970376-91970395 MsG0580029101.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr5:91970326-91970345 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr5:91970899-91970918 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr5:91970359-91970378 MsG0580029101.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG - Chr5:91970443-91970462 MsG0580029101.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG - Chr5:91970272-91970291 MsG0580029101.01.T01:CDS 30.0%
CATGGAGATACAATTAAAGA+AGG - Chr5:91970246-91970265 MsG0580029101.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG - Chr5:91971199-91971218 MsG0580029101.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr5:91971055-91971074 MsG0580029101.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG - Chr5:91970473-91970492 MsG0580029101.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr5:91970577-91970596 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr5:91970358-91970377 MsG0580029101.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr5:91970369-91970388 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr5:91970358-91970377 None:intergenic 30.0%
! GCTTTTGATGAAAAATCACT+CGG - Chr5:91970683-91970702 MsG0580029101.01.T01:CDS 30.0%
! TGGAAATAATTATCTGGCTT+GGG - Chr5:91970188-91970207 MsG0580029101.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG - Chr5:91970168-91970187 MsG0580029101.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr5:91970323-91970342 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG - Chr5:91970849-91970868 MsG0580029101.01.T01:CDS 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG + Chr5:91970617-91970636 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr5:91971058-91971077 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr5:91970429-91970448 None:intergenic 35.0%
AACTGAACAACAAAAGGCTA+AGG - Chr5:91970278-91970297 MsG0580029101.01.T01:CDS 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr5:91970510-91970529 MsG0580029101.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr5:91970518-91970537 MsG0580029101.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr5:91970425-91970444 None:intergenic 35.0%
CTGGAAATAATTATCTGGCT+TGG - Chr5:91970187-91970206 MsG0580029101.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr5:91970424-91970443 MsG0580029101.01.T01:CDS 35.0%
! AGATGGTCAATCTTTTCATC+AGG + Chr5:91971179-91971198 None:intergenic 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG + Chr5:91970155-91970174 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG - Chr5:91970152-91970171 MsG0580029101.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr5:91970353-91970372 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG - Chr5:91970613-91970632 MsG0580029101.01.T01:CDS 35.0%
AATTGTATCTCCATGCCCTT+CGG + Chr5:91970241-91970260 None:intergenic 40.0%
AGAAATCAGCAGCATCTAAG+TGG + Chr5:91971148-91971167 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG - Chr5:91970603-91970622 MsG0580029101.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG - Chr5:91970644-91970663 MsG0580029101.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG + Chr5:91971147-91971166 None:intergenic 40.0%
GATCACTATGGACAAAATCG+TGG - Chr5:91970756-91970775 MsG0580029101.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG - Chr5:91970744-91970763 MsG0580029101.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr5:91970407-91970426 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG + Chr5:91970734-91970753 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG + Chr5:91970659-91970678 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG + Chr5:91970735-91970754 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr5:91970978-91970997 MsG0580029101.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr5:91970975-91970994 MsG0580029101.01.T01:CDS 40.0%
CATTTAAATGCCGAAGGGCA+TGG - Chr5:91970228-91970247 MsG0580029101.01.T01:CDS 45.0%
GCAGCAACAATACCGTGAAA+AGG - Chr5:91970602-91970621 MsG0580029101.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr5:91970991-91971010 MsG0580029101.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG - Chr5:91970762-91970781 MsG0580029101.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr5:91970984-91971003 MsG0580029101.01.T01:CDS 45.0%
TTTCACGGTATTGTTGCTGC+AGG + Chr5:91970602-91970621 None:intergenic 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr5:91970729-91970748 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG + Chr5:91970304-91970323 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr5:91971024-91971043 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG - Chr5:91970815-91970834 MsG0580029101.01.T01:CDS 50.0%
CCTTCGTCACCACCTTCATA+AGG - Chr5:91970311-91970330 MsG0580029101.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr5:91970314-91970333 None:intergenic 50.0%
! TATGGAAGGGGACGTGGTTT+TGG - Chr5:91970828-91970847 MsG0580029101.01.T01:CDS 50.0%
CGTGGTCGTGGTTATGGAAG+GGG - Chr5:91970816-91970835 MsG0580029101.01.T01:CDS 55.0%
GACGTGGTCGTGGTTATGGA+AGG - Chr5:91970814-91970833 MsG0580029101.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr5:91970714-91970733 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr5:91970715-91970734 None:intergenic 55.0%
CAAAATCGTGGTCGTGGCCG+TGG - Chr5:91970768-91970787 MsG0580029101.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG + Chr5:91970788-91970807 None:intergenic 60.0%
CGTGGACGTGGTCGTGGTTA+TGG - Chr5:91970810-91970829 MsG0580029101.01.T01:CDS 60.0%
CGTGGTTATGGAAGGGGACG+TGG - Chr5:91970822-91970841 MsG0580029101.01.T01:CDS 60.0%
! AATCACTCGGCTCGTCCCAC+TGG - Chr5:91970696-91970715 MsG0580029101.01.T01:CDS 60.0%
CATGGTCGTGGACGTGGACG+TGG - Chr5:91970792-91970811 MsG0580029101.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG - Chr5:91970786-91970805 MsG0580029101.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGACG+TGG - Chr5:91970798-91970817 MsG0580029101.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG - Chr5:91970804-91970823 MsG0580029101.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG - Chr5:91970780-91970799 MsG0580029101.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG - Chr5:91970774-91970793 MsG0580029101.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 91970144 91971226 91970144 ID=MsG0580029101.01;Name=MsG0580029101.01
Chr5 mRNA 91970144 91971226 91970144 ID=MsG0580029101.01.T01;Parent=MsG0580029101.01;Name=MsG0580029101.01.T01;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|360
Chr5 exon 91970144 91971226 91970144 ID=MsG0580029101.01.T01:exon:7459;Parent=MsG0580029101.01.T01
Chr5 CDS 91970144 91971226 91970144 ID=MsG0580029101.01.T01:cds;Parent=MsG0580029101.01.T01
Gene Sequence

>MsG0580029101.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0580029101.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*