AlfalfaGEDB | MsG0580029101.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029101.01

KEGG: -
GO: GO:0003676 GO:0008270
PFAM: PF00098 PF14223

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029101.01.T01	XP_039688731.1	64.402	368	102	5	1	360	1	347	1.08E-152	444

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029101.01.T01	A0A6D2I977	60.112	356	108	6	1	343	1	335	1.61e-138	406

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280006510.01	MsG0580029101.01	0.863206	2.936372e-64	8.830985e-61
MsG0280010102.01	MsG0580029101.01	0.802141	6.459712e-49	3.140329e-46
MsG0380012021.01	MsG0580029101.01	0.831342	1.762293e-55	1.906967e-52
MsG0380012033.01	MsG0580029101.01	0.814130	1.802097e-51	1.199119e-48
MsG0380014122.01	MsG0580029101.01	0.981711	2.934211e-153	7.822578e-147
MsG0380015877.01	MsG0580029101.01	0.814668	1.370463e-51	9.252624e-49
MsG0380017940.01	MsG0580029101.01	0.805004	1.645536e-49	8.609174e-47
MsG0480021775.01	MsG0580029101.01	0.885985	5.068229e-72	3.553403e-68
MsG0480022862.01	MsG0580029101.01	0.962251	1.179472e-120	5.831544e-115
MsG0480023011.01	MsG0580029101.01	0.806981	6.317369e-50	3.477130e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029101.01.T01	MTR_7g062720	65.909	132	45	0	1	132	1	132	9.98e-60	190
MsG0580029101.01.T01	MTR_5g061150	62.992	127	47	0	20	146	10	136	2.18e-48	160
MsG0580029101.01.T01	MTR_4g037355	57.971	138	35	5	151	287	1	116	2.52e-41	142
MsG0580029101.01.T01	MTR_4g112730	71.831	71	18	1	138	208	1	69	1.03e-26	102
MsG0580029101.01.T01	MTR_1g036690	58.537	82	27	3	280	360	5	80	1.06e-22	90.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCATTTAAATGCCGA+AGG	0.537172	5:-91971126	MsG0580029101.01.T01:CDS
AAATTCATTTAAATGCCGAA+GGG	0.631745	5:-91971125	MsG0580029101.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG	0.267031	5:+91970982	None:intergenic
AACCATTCATTACGAGCATT+AGG	0.386252	5:+91970922	None:intergenic
AACTGAACAACAAAAGGCTA+AGG	0.582581	5:-91971070	MsG0580029101.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG	0.475930	5:-91970652	MsG0580029101.01.T01:CDS
AATGTTGTAAAGAATGATAT+TGG	0.471241	5:-91970415	MsG0580029101.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG	0.460742	5:+91971110	None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG	0.598966	5:-91970533	MsG0580029101.01.T01:CDS
ACTTCAAAATAAGGATAAAA+GGG	0.487291	5:-91970284	MsG0580029101.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGATCCAAAAGATCTTT+GGG	-	Chr5:91970359-91970378	MsG0580029101.01.T01:CDS	30.0%
ATGGTTATATTTGCGTTTAC+AGG	-	Chr5:91970443-91970462	MsG0580029101.01.T01:CDS	30.0%
CAAAACAACTGAACAACAAA+AGG	-	Chr5:91970272-91970291	MsG0580029101.01.T01:CDS	30.0%
CATGGAGATACAATTAAAGA+AGG	-	Chr5:91970246-91970265	MsG0580029101.01.T01:CDS	30.0%
CATTAGAGATGAAAATATCC+AGG	-	Chr5:91971199-91971218	MsG0580029101.01.T01:CDS	30.0%
CCAACAATCACTTCAAAATA+AGG	-	Chr5:91971055-91971074	MsG0580029101.01.T01:CDS	30.0%
GTCTGTAATCGAATATAACT+CGG	-	Chr5:91970473-91970492	MsG0580029101.01.T01:CDS	30.0%
TACATTAGATGCATGAAAAG+TGG	+	Chr5:91970577-91970596	None:intergenic	30.0%
TAGAAGATCCAAAAGATCTT+TGG	-	Chr5:91970358-91970377	MsG0580029101.01.T01:CDS	30.0%
AACCATTCATTACGAGCATT+AGG	+	Chr5:91970429-91970448	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	91970144	91971226	91970144	ID=MsG0580029101.01;Name=MsG0580029101.01
Chr5	mRNA	91970144	91971226	91970144	ID=MsG0580029101.01.T01;Parent=MsG0580029101.01;Name=MsG0580029101.01.T01;_AED=0.24;_eAED=0.24;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|360
Chr5	exon	91970144	91971226	91970144	ID=MsG0580029101.01.T01:exon:7459;Parent=MsG0580029101.01.T01
Chr5	CDS	91970144	91971226	91970144	ID=MsG0580029101.01.T01:cds;Parent=MsG0580029101.01.T01

Gene Sequence

>MsG0580029101.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0580029101.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*