AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014122.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014122.01.T01 MTR_7g062720 65.909 132 45 0 1 132 1 132 9.06e-60 190
MsG0380014122.01.T01 MTR_5g061150 62.992 127 47 0 20 146 10 136 2.58e-48 160
MsG0380014122.01.T01 MTR_4g037355 57.246 138 36 4 151 287 1 116 2.80e-41 142
MsG0380014122.01.T01 MTR_4g112730 69.863 73 20 1 138 210 1 71 1.80e-25 99.0
MsG0380014122.01.T01 MTR_1g036690 58.537 82 27 3 280 360 5 80 1.05e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 3:-50142558 MsG0380014122.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 3:+50142506 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 3:-50141663 MsG0380014122.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 3:-50141671 MsG0380014122.01.T01:CDS
TGTGGAAGGGGACGTGGTTT+TGG 0.247927 3:-50141898 MsG0380014122.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 3:-50142283 MsG0380014122.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 3:+50142360 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 3:-50142544 MsG0380014122.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 3:-50142368 MsG0380014122.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 3:+50141994 None:intergenic
TATAGGTGGGGTTCTATTCC+TGG 0.331534 3:+50141509 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 3:+50141694 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 3:-50141611 MsG0380014122.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 3:-50142539 MsG0380014122.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.353242 3:-50142538 MsG0380014122.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 3:+50142300 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 3:-50141619 MsG0380014122.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 3:-50142350 MsG0380014122.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 3:-50142367 MsG0380014122.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 3:-50141742 MsG0380014122.01.T01:CDS
CGTGGTCGTGGTTGTGGAAG+GGG 0.421350 3:-50141910 MsG0380014122.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 3:+50141995 None:intergenic
CGTGGTTGTGGAAGGGGACG+TGG 0.427760 3:-50141904 MsG0380014122.01.T01:CDS
CCTTCGTCACCACCTTCATA+AGG 0.433246 3:-50142415 MsG0380014122.01.T01:CDS
CGTGGACGTGGTCGTGGTTG+TGG 0.436225 3:-50141916 MsG0380014122.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 3:-50142208 MsG0380014122.01.T01:CDS
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 3:-50141958 MsG0380014122.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 3:-50141952 MsG0380014122.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 3:-50142454 MsG0380014122.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG 0.460742 3:+50142488 None:intergenic
AGAAATCAGCAGCATCTAAG+TGG 0.464577 3:+50141581 None:intergenic
TTTCACGGTATTGTTGCTGC+AGG 0.465441 3:+50142127 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 3:-50141793 MsG0380014122.01.T01:CDS
GCAGCAACAATACCGTGAAA+AGG 0.474876 3:-50142124 MsG0380014122.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG 0.475930 3:-50142030 MsG0380014122.01.T01:CDS
GACGTGGTCGTGGTTGTGGA+AGG 0.480300 3:-50141912 MsG0380014122.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.483138 3:+50142000 None:intergenic
ACGTGGTCGTGGTTGTGGAA+GGG 0.484449 3:-50141911 MsG0380014122.01.T01:CDS
ACTTCAAAATAAGGATAAAA+GGG 0.487291 3:-50141662 MsG0380014122.01.T01:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 3:-50142498 MsG0380014122.01.T01:CDS
CAGCAACAATACCGTGAAAA+GGG 0.498612 3:-50142123 MsG0380014122.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 3:-50141982 MsG0380014122.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 3:-50141735 MsG0380014122.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 3:-50141946 MsG0380014122.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 3:-50142302 MsG0380014122.01.T01:CDS
TACATTAGATGCATGAAAAG+TGG 0.516304 3:+50142152 None:intergenic
CGTGGACGTGGACGTGGTCG+TGG 0.520243 3:-50141922 MsG0380014122.01.T01:CDS
CATGGTCGTGGACGTGGACG+TGG 0.524744 3:-50141934 MsG0380014122.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 3:-50141527 MsG0380014122.01.T01:CDS
CATGGAGATACAATTAAAGA+AGG 0.533844 3:-50142480 MsG0380014122.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 3:-50142504 MsG0380014122.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 3:-50141940 MsG0380014122.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 3:-50141928 MsG0380014122.01.T01:CDS
CTTAATATCATGCCTGCTTG+TGG 0.567363 3:-50142082 MsG0380014122.01.T01:CDS
TATGGACAAAATCGTGGTCG+TGG 0.573891 3:-50141964 MsG0380014122.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 3:-50142253 MsG0380014122.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 3:-50142448 MsG0380014122.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 3:-50141751 MsG0380014122.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 3:+50142304 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 3:+50142415 None:intergenic
AGTATTTGCTATCGTTGTGG+AGG 0.599695 3:-50141748 MsG0380014122.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 3:+50141582 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 3:-50142503 MsG0380014122.01.T01:CDS
CACGTCCACGACCATGACCA+CGG 0.661673 3:+50141941 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 3:+50142425 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 3:-50142216 MsG0380014122.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 3:+50142014 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 3:+50142015 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 3:-50141970 MsG0380014122.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr3:50142311-50142330 MsG0380014122.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr3:50142442-50142461 MsG0380014122.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG - Chr3:50141560-50141579 MsG0380014122.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG - Chr3:50141600-50141619 MsG0380014122.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG - Chr3:50141601-50141620 MsG0380014122.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr3:50142441-50142460 MsG0380014122.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr3:50142485-50142504 MsG0380014122.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr3:50142493-50142512 MsG0380014122.01.T01:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr3:50142413-50142432 None:intergenic 25.0%
!! TATTTTCATCTCTAATGAGA+TGG + Chr3:50142574-50142593 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr3:50142301-50142320 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG + Chr3:50141601-50141620 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG - Chr3:50141754-50141773 MsG0380014122.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr3:50141704-50141723 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr3:50142277-50142296 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr3:50141737-50141756 MsG0380014122.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG - Chr3:50141821-50141840 MsG0380014122.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG - Chr3:50141650-50141669 MsG0380014122.01.T01:CDS 30.0%
CATGGAGATACAATTAAAGA+AGG - Chr3:50141624-50141643 MsG0380014122.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG - Chr3:50142577-50142596 MsG0380014122.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr3:50142433-50142452 MsG0380014122.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG - Chr3:50141851-50141870 MsG0380014122.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr3:50141955-50141974 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr3:50141736-50141755 MsG0380014122.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr3:50141747-50141766 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr3:50141736-50141755 None:intergenic 30.0%
! GCTTTTGATGAAAAATCACT+CGG - Chr3:50142061-50142080 MsG0380014122.01.T01:CDS 30.0%
! TGGAAATAATTATCTGGCTT+GGG - Chr3:50141566-50141585 MsG0380014122.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG - Chr3:50141546-50141565 MsG0380014122.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr3:50141701-50141720 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG - Chr3:50142227-50142246 MsG0380014122.01.T01:CDS 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG + Chr3:50141995-50142014 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr3:50142436-50142455 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr3:50141807-50141826 None:intergenic 35.0%
AACTGAACAACAAAAGGCTA+AGG - Chr3:50141656-50141675 MsG0380014122.01.T01:CDS 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr3:50141888-50141907 MsG0380014122.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr3:50141896-50141915 MsG0380014122.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr3:50141803-50141822 None:intergenic 35.0%
CTGGAAATAATTATCTGGCT+TGG - Chr3:50141565-50141584 MsG0380014122.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr3:50141802-50141821 MsG0380014122.01.T01:CDS 35.0%
! AGATGGTCAATCTTTTCATC+AGG + Chr3:50142557-50142576 None:intergenic 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG + Chr3:50141533-50141552 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG - Chr3:50141530-50141549 MsG0380014122.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr3:50141731-50141750 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG - Chr3:50141991-50142010 MsG0380014122.01.T01:CDS 35.0%
AATTGTATCTCCATGCCCTT+CGG + Chr3:50141619-50141638 None:intergenic 40.0%
AGAAATCAGCAGCATCTAAG+TGG + Chr3:50142526-50142545 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG - Chr3:50141981-50142000 MsG0380014122.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG - Chr3:50142022-50142041 MsG0380014122.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG + Chr3:50142525-50142544 None:intergenic 40.0%
GATCACTATGGACAAAATCG+TGG - Chr3:50142134-50142153 MsG0380014122.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG - Chr3:50142122-50142141 MsG0380014122.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr3:50141785-50141804 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG + Chr3:50142112-50142131 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG + Chr3:50142037-50142056 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG + Chr3:50142113-50142132 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr3:50142356-50142375 MsG0380014122.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr3:50142353-50142372 MsG0380014122.01.T01:CDS 40.0%
CATTTAAATGCCGAAGGGCA+TGG - Chr3:50141606-50141625 MsG0380014122.01.T01:CDS 45.0%
GCAGCAACAATACCGTGAAA+AGG - Chr3:50141980-50141999 MsG0380014122.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr3:50142369-50142388 MsG0380014122.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG - Chr3:50142140-50142159 MsG0380014122.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr3:50142362-50142381 MsG0380014122.01.T01:CDS 45.0%
TTTCACGGTATTGTTGCTGC+AGG + Chr3:50141980-50141999 None:intergenic 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr3:50142107-50142126 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG + Chr3:50141682-50141701 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr3:50142402-50142421 None:intergenic 45.0%
CCTTCGTCACCACCTTCATA+AGG - Chr3:50141689-50141708 MsG0380014122.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr3:50141692-50141711 None:intergenic 50.0%
ACGTGGTCGTGGTTGTGGAA+GGG - Chr3:50142193-50142212 MsG0380014122.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr3:50142092-50142111 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr3:50142093-50142112 None:intergenic 55.0%
! TGTGGAAGGGGACGTGGTTT+TGG - Chr3:50142206-50142225 MsG0380014122.01.T01:CDS 55.0%
CAAAATCGTGGTCGTGGCCG+TGG - Chr3:50142146-50142165 MsG0380014122.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG + Chr3:50142166-50142185 None:intergenic 60.0%
CGTGGTCGTGGTTGTGGAAG+GGG - Chr3:50142194-50142213 MsG0380014122.01.T01:CDS 60.0%
GACGTGGTCGTGGTTGTGGA+AGG - Chr3:50142192-50142211 MsG0380014122.01.T01:CDS 60.0%
! AATCACTCGGCTCGTCCCAC+TGG - Chr3:50142074-50142093 MsG0380014122.01.T01:CDS 60.0%
CATGGTCGTGGACGTGGACG+TGG - Chr3:50142170-50142189 MsG0380014122.01.T01:CDS 65.0%
CGTGGACGTGGTCGTGGTTG+TGG - Chr3:50142188-50142207 MsG0380014122.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG - Chr3:50142164-50142183 MsG0380014122.01.T01:CDS 65.0%
CGTGGTTGTGGAAGGGGACG+TGG - Chr3:50142200-50142219 MsG0380014122.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGACG+TGG - Chr3:50142176-50142195 MsG0380014122.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG - Chr3:50142182-50142201 MsG0380014122.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG - Chr3:50142158-50142177 MsG0380014122.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG - Chr3:50142152-50142171 MsG0380014122.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 50141522 50142604 50141522 ID=MsG0380014122.01;Name=MsG0380014122.01
Chr3 mRNA 50141522 50142604 50141522 ID=MsG0380014122.01.T01;Parent=MsG0380014122.01;Name=MsG0380014122.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|360
Chr3 exon 50141522 50142604 50141522 ID=MsG0380014122.01.T01:exon:9175;Parent=MsG0380014122.01.T01
Chr3 CDS 50141522 50142604 50141522 ID=MsG0380014122.01.T01:cds;Parent=MsG0380014122.01.T01
Gene Sequence

>MsG0380014122.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCCTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTGTGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0380014122.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGRGCGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*