AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022862.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022862.01.T01 MTR_7g062720 65.909 132 45 0 1 132 1 132 9.98e-60 190
MsG0480022862.01.T01 MTR_5g061150 62.992 127 47 0 20 146 10 136 2.18e-48 160
MsG0480022862.01.T01 MTR_4g037355 57.971 138 35 5 151 287 1 116 2.52e-41 142
MsG0480022862.01.T01 MTR_4g112730 71.831 71 18 1 138 208 1 69 1.03e-26 102
MsG0480022862.01.T01 MTR_1g036690 58.537 82 27 3 280 360 5 80 1.06e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 4:-77677492 MsG0480022862.01.T01:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 4:+77677440 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 4:-77676597 MsG0480022862.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 4:-77676605 MsG0480022862.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 4:-77677217 MsG0480022862.01.T01:CDS
TATGGAAGGGGACGTGGTTT+TGG 0.259444 4:-77676832 MsG0480022862.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.267031 4:+77677294 None:intergenic
TATTTCTGGAAATAATTATC+TGG 0.282933 4:-77677478 MsG0480022862.01.T01:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.283634 4:-77677302 MsG0480022862.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 4:+77676928 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 4:+77676628 None:intergenic
TATAGGCGGGGTTCTATTCC+TGG 0.336951 4:+77676443 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 4:-77676545 MsG0480022862.01.T01:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 4:-77677473 MsG0480022862.01.T01:CDS
TGGAAATAATTATCTGGCTT+GGG 0.353242 4:-77677472 MsG0480022862.01.T01:CDS
CGTGGACGTGGTCGTGGTTA+TGG 0.362984 4:-77676850 MsG0480022862.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 4:+77677234 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 4:-77676553 MsG0480022862.01.T01:CDS
TTTGGGACAATTTAAAAGAA+AGG 0.395342 4:-77677284 MsG0480022862.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 4:-77677301 MsG0480022862.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 4:-77676676 MsG0480022862.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 4:+77676929 None:intergenic
CGTGGTTATGGAAGGGGACG+TGG 0.429618 4:-77676838 MsG0480022862.01.T01:CDS
CCTTCGTCACCACCTTCATA+AGG 0.433246 4:-77677349 MsG0480022862.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 4:-77677142 MsG0480022862.01.T01:CDS
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 4:-77676892 MsG0480022862.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 4:-77676886 MsG0480022862.01.T01:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 4:-77677388 MsG0480022862.01.T01:CDS
AATTGTATCTCCATGCCCTT+CGG 0.460742 4:+77677422 None:intergenic
AGAAATCAGCAGCATCTAAG+TGG 0.464577 4:+77676515 None:intergenic
GCAGCAACAATACCGTGAAA+AGG 0.470239 4:-77677058 MsG0480022862.01.T01:CDS
AATGTTGTAAAGAATGATAT+TGG 0.471241 4:-77676727 MsG0480022862.01.T01:CDS
AATCACTCGGCTCGTCCCAC+TGG 0.475930 4:-77676964 MsG0480022862.01.T01:CDS
CAGCAACAATACCGTGAAAA+GGG 0.478771 4:-77677057 MsG0480022862.01.T01:CDS
GCTACATTCACTTCTGGGAA+TGG 0.483138 4:+77676934 None:intergenic
ACTTCAAAATAAGGATAAAA+GGG 0.487291 4:-77676596 MsG0480022862.01.T01:CDS
TACATTAGATGCATGAAAAG+TGG 0.490606 4:+77677086 None:intergenic
CATTTAAATGCCGAAGGGCA+TGG 0.494088 4:-77677432 MsG0480022862.01.T01:CDS
GACGTGGTCGTGGTTATGGA+AGG 0.501950 4:-77676846 MsG0480022862.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 4:-77676916 MsG0480022862.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 4:-77676669 MsG0480022862.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 4:-77676880 MsG0480022862.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 4:-77677236 MsG0480022862.01.T01:CDS
CATGGTCGTGGACGTGGACG+TGG 0.524744 4:-77676868 MsG0480022862.01.T01:CDS
CGTGGACGTGGACGTGGTCG+TGG 0.524981 4:-77676856 MsG0480022862.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 4:-77676461 MsG0480022862.01.T01:CDS
CATGGAGATACAATTAAAGA+AGG 0.533844 4:-77677414 MsG0480022862.01.T01:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 4:-77677438 MsG0480022862.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 4:-77676874 MsG0480022862.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.546682 4:-77676862 MsG0480022862.01.T01:CDS
CGTGGTCGTGGTTATGGAAG+GGG 0.551769 4:-77676844 MsG0480022862.01.T01:CDS
CTTAATATCATGCCTGCTTG+TGG 0.567363 4:-77677016 MsG0480022862.01.T01:CDS
TATGGACAAAATCGTGGTCG+TGG 0.573891 4:-77676898 MsG0480022862.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 4:-77677187 MsG0480022862.01.T01:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 4:-77677382 MsG0480022862.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 4:-77676685 MsG0480022862.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 4:+77677238 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 4:+77677349 None:intergenic
ACGTGGTCGTGGTTATGGAA+GGG 0.598966 4:-77676845 MsG0480022862.01.T01:CDS
AGTATTTGCTATCGTTGTGG+AGG 0.599695 4:-77676682 MsG0480022862.01.T01:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 4:+77676516 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 4:-77677437 MsG0480022862.01.T01:CDS
CACGTCCACGACCATGACCA+CGG 0.661673 4:+77676875 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 4:+77677359 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 4:-77677150 MsG0480022862.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 4:+77676948 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 4:+77676949 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 4:-77676904 MsG0480022862.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr4:77677245-77677264 MsG0480022862.01.T01:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr4:77677376-77677395 MsG0480022862.01.T01:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG - Chr4:77676494-77676513 MsG0480022862.01.T01:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG - Chr4:77676534-77676553 MsG0480022862.01.T01:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG - Chr4:77676535-77676554 MsG0480022862.01.T01:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr4:77677375-77677394 MsG0480022862.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr4:77677419-77677438 MsG0480022862.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr4:77677427-77677446 MsG0480022862.01.T01:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr4:77677347-77677366 None:intergenic 25.0%
!! TATTTTCATCTCTAATGAGA+TGG + Chr4:77677508-77677527 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr4:77677235-77677254 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG + Chr4:77676535-77676554 None:intergenic 25.0%
!! TTTGGGACAATTTAAAAGAA+AGG - Chr4:77676688-77676707 MsG0480022862.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr4:77676638-77676657 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr4:77677211-77677230 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr4:77676671-77676690 MsG0480022862.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG - Chr4:77676755-77676774 MsG0480022862.01.T01:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG - Chr4:77676584-77676603 MsG0480022862.01.T01:CDS 30.0%
CATGGAGATACAATTAAAGA+AGG - Chr4:77676558-77676577 MsG0480022862.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG - Chr4:77677511-77677530 MsG0480022862.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr4:77677367-77677386 MsG0480022862.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG - Chr4:77676785-77676804 MsG0480022862.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr4:77676889-77676908 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr4:77676670-77676689 MsG0480022862.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr4:77676681-77676700 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr4:77676670-77676689 None:intergenic 30.0%
! GCTTTTGATGAAAAATCACT+CGG - Chr4:77676995-77677014 MsG0480022862.01.T01:CDS 30.0%
! TGGAAATAATTATCTGGCTT+GGG - Chr4:77676500-77676519 MsG0480022862.01.T01:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG - Chr4:77676480-77676499 MsG0480022862.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr4:77676635-77676654 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG - Chr4:77677161-77677180 MsG0480022862.01.T01:CDS 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG + Chr4:77676929-77676948 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr4:77677370-77677389 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr4:77676741-77676760 None:intergenic 35.0%
AACTGAACAACAAAAGGCTA+AGG - Chr4:77676590-77676609 MsG0480022862.01.T01:CDS 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr4:77676822-77676841 MsG0480022862.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr4:77676830-77676849 MsG0480022862.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr4:77676737-77676756 None:intergenic 35.0%
CTGGAAATAATTATCTGGCT+TGG - Chr4:77676499-77676518 MsG0480022862.01.T01:CDS 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr4:77676736-77676755 MsG0480022862.01.T01:CDS 35.0%
! AGATGGTCAATCTTTTCATC+AGG + Chr4:77677491-77677510 None:intergenic 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG + Chr4:77676467-77676486 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG - Chr4:77676464-77676483 MsG0480022862.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr4:77676665-77676684 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG - Chr4:77676925-77676944 MsG0480022862.01.T01:CDS 35.0%
AATTGTATCTCCATGCCCTT+CGG + Chr4:77676553-77676572 None:intergenic 40.0%
AGAAATCAGCAGCATCTAAG+TGG + Chr4:77677460-77677479 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG - Chr4:77676915-77676934 MsG0480022862.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG - Chr4:77676956-77676975 MsG0480022862.01.T01:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG + Chr4:77677459-77677478 None:intergenic 40.0%
GATCACTATGGACAAAATCG+TGG - Chr4:77677068-77677087 MsG0480022862.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG - Chr4:77677056-77677075 MsG0480022862.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr4:77676719-77676738 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG + Chr4:77677046-77677065 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG + Chr4:77676971-77676990 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG + Chr4:77677047-77677066 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr4:77677290-77677309 MsG0480022862.01.T01:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr4:77677287-77677306 MsG0480022862.01.T01:CDS 40.0%
CATTTAAATGCCGAAGGGCA+TGG - Chr4:77676540-77676559 MsG0480022862.01.T01:CDS 45.0%
GCAGCAACAATACCGTGAAA+AGG - Chr4:77676914-77676933 MsG0480022862.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr4:77677303-77677322 MsG0480022862.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG - Chr4:77677074-77677093 MsG0480022862.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr4:77677296-77677315 MsG0480022862.01.T01:CDS 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr4:77677041-77677060 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG + Chr4:77676616-77676635 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr4:77677336-77677355 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG - Chr4:77677127-77677146 MsG0480022862.01.T01:CDS 50.0%
CCTTCGTCACCACCTTCATA+AGG - Chr4:77676623-77676642 MsG0480022862.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr4:77676626-77676645 None:intergenic 50.0%
! TATGGAAGGGGACGTGGTTT+TGG - Chr4:77677140-77677159 MsG0480022862.01.T01:CDS 50.0%
CGTGGTCGTGGTTATGGAAG+GGG - Chr4:77677128-77677147 MsG0480022862.01.T01:CDS 55.0%
GACGTGGTCGTGGTTATGGA+AGG - Chr4:77677126-77677145 MsG0480022862.01.T01:CDS 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr4:77677026-77677045 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr4:77677027-77677046 None:intergenic 55.0%
CAAAATCGTGGTCGTGGCCG+TGG - Chr4:77677080-77677099 MsG0480022862.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG + Chr4:77677100-77677119 None:intergenic 60.0%
CGTGGACGTGGTCGTGGTTA+TGG - Chr4:77677122-77677141 MsG0480022862.01.T01:CDS 60.0%
CGTGGTTATGGAAGGGGACG+TGG - Chr4:77677134-77677153 MsG0480022862.01.T01:CDS 60.0%
! AATCACTCGGCTCGTCCCAC+TGG - Chr4:77677008-77677027 MsG0480022862.01.T01:CDS 60.0%
CATGGTCGTGGACGTGGACG+TGG - Chr4:77677104-77677123 MsG0480022862.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG - Chr4:77677098-77677117 MsG0480022862.01.T01:CDS 65.0%
CGTGGACGTGGACGTGGACG+TGG - Chr4:77677110-77677129 MsG0480022862.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGTCG+TGG - Chr4:77677116-77677135 MsG0480022862.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG - Chr4:77677092-77677111 MsG0480022862.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG - Chr4:77677086-77677105 MsG0480022862.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 77676456 77677538 77676456 ID=MsG0480022862.01;Name=MsG0480022862.01
Chr4 mRNA 77676456 77677538 77676456 ID=MsG0480022862.01.T01;Parent=MsG0480022862.01;Name=MsG0480022862.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|360
Chr4 exon 77676456 77677538 77676456 ID=MsG0480022862.01.T01:exon:4897;Parent=MsG0480022862.01.T01
Chr4 CDS 77676456 77677538 77676456 ID=MsG0480022862.01.T01:cds;Parent=MsG0480022862.01.T01
Gene Sequence

>MsG0480022862.01.T01

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCTCTAGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACAATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGGTGACTGATGCGCAGATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCTTGCAGCAACAATACCGTGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGCCTGCTTGTGGCAGAACAAAATAACGAGCTTTTGATGAAAAATCACTCGGCTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0480022862.01.T01

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWDNLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHSARPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGRGYGRGRGFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE*