Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029563.01.T01 | XP_024640218.1 | 93.69 | 729 | 46 | 0 | 1 | 729 | 151 | 879 | 0 | 1419 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048892.01 | MsG0580029563.01 | 0.802658 | 5.053900e-49 | 2.489492e-46 |
| MsG0180000142.01 | MsG0580029563.01 | 0.828352 | 9.428448e-55 | 9.347796e-52 |
| MsG0180000444.01 | MsG0580029563.01 | 0.804811 | 1.805483e-49 | 9.399917e-47 |
| MsG0180001173.01 | MsG0580029563.01 | 0.801248 | 9.849145e-49 | 4.681153e-46 |
| MsG0180003073.01 | MsG0580029563.01 | 0.844871 | 5.780492e-59 | 9.460015e-56 |
| MsG0180003097.01 | MsG0580029563.01 | 0.830348 | 3.088953e-55 | 3.246092e-52 |
| MsG0180003786.01 | MsG0580029563.01 | 0.815305 | 9.895560e-52 | 6.797547e-49 |
| MsG0180004991.01 | MsG0580029563.01 | 0.806671 | 7.344263e-50 | 4.009930e-47 |
| MsG0180005664.01 | MsG0580029563.01 | 0.804543 | 2.054289e-49 | 1.062025e-46 |
| MsG0180006023.01 | MsG0580029563.01 | 0.816651 | 4.955507e-52 | 3.531935e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029563.01.T01 | MTR_5g088490 | 93.690 | 729 | 46 | 0 | 1 | 729 | 151 | 879 | 0.0 | 1419 |
| MsG0580029563.01.T01 | MTR_5g088510 | 74.095 | 718 | 184 | 2 | 1 | 716 | 161 | 878 | 0.0 | 1067 |
| MsG0580029563.01.T01 | MTR_5g095130 | 23.769 | 711 | 452 | 14 | 26 | 723 | 174 | 807 | 9.39e-43 | 167 |
| MsG0580029563.01.T01 | MTR_5g095130 | 22.702 | 718 | 461 | 16 | 16 | 719 | 234 | 871 | 3.71e-39 | 156 |
| MsG0580029563.01.T01 | MTR_5g095130 | 21.382 | 608 | 467 | 9 | 130 | 729 | 174 | 778 | 3.07e-27 | 119 |
| MsG0580029563.01.T01 | MTR_5g095130 | 22.885 | 520 | 348 | 11 | 251 | 723 | 154 | 667 | 4.46e-22 | 102 |
| MsG0580029563.01.T01 | MTR_5g095130 | 25.000 | 292 | 217 | 2 | 20 | 310 | 587 | 877 | 1.32e-15 | 81.6 |
| MsG0580029563.01.T01 | MTR_3g105900 | 20.978 | 777 | 534 | 11 | 25 | 722 | 310 | 1085 | 1.10e-38 | 155 |
| MsG0580029563.01.T01 | MTR_3g105900 | 23.108 | 740 | 504 | 17 | 26 | 711 | 241 | 969 | 6.12e-35 | 143 |
| MsG0580029563.01.T01 | MTR_3g105900 | 21.781 | 730 | 505 | 15 | 4 | 715 | 432 | 1113 | 8.31e-34 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029563.01.T01 | AT1G71210 | 41.326 | 709 | 400 | 5 | 1 | 694 | 162 | 869 | 1.37e-175 | 525 |
| MsG0580029563.01.T01 | AT3G06920 | 23.505 | 736 | 483 | 13 | 26 | 719 | 153 | 850 | 1.64e-47 | 181 |
| MsG0580029563.01.T01 | AT3G06920 | 23.394 | 607 | 404 | 11 | 3 | 559 | 200 | 795 | 1.05e-35 | 145 |
| MsG0580029563.01.T01 | AT3G06920 | 21.785 | 661 | 439 | 14 | 58 | 711 | 115 | 704 | 8.52e-28 | 120 |
| MsG0580029563.01.T01 | AT3G06920 | 24.183 | 306 | 226 | 4 | 8 | 310 | 554 | 856 | 2.26e-15 | 80.9 |
| MsG0580029563.01.T01 | AT3G06920 | 23.505 | 736 | 483 | 13 | 26 | 719 | 153 | 850 | 1.64e-47 | 181 |
| MsG0580029563.01.T01 | AT3G06920 | 23.394 | 607 | 404 | 11 | 3 | 559 | 200 | 795 | 1.05e-35 | 145 |
| MsG0580029563.01.T01 | AT3G06920 | 21.785 | 661 | 439 | 14 | 58 | 711 | 115 | 704 | 8.52e-28 | 120 |
| MsG0580029563.01.T01 | AT3G06920 | 24.183 | 306 | 226 | 4 | 8 | 310 | 554 | 856 | 2.26e-15 | 80.9 |
| MsG0580029563.01.T01 | AT3G06920 | 23.505 | 736 | 483 | 13 | 26 | 719 | 139 | 836 | 2.16e-47 | 181 |
Find 154 sgRNAs with CRISPR-Local
Find 182 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCACGGGAGAAATGATG+AGG | 0.665268 | 5:+98622164 | MsG0580029563.01.T01:CDS |
| AAATCCTTCTGTTGAGGAAT+TGG | 0.308783 | 5:+98621972 | MsG0580029563.01.T01:CDS |
| AAATGATGAGGCTAAGCAGT+GGG | 0.615788 | 5:+98622176 | MsG0580029563.01.T01:CDS |
| AAATTATCTTATCGGTTTGT+TGG | 0.333992 | 5:+98620424 | MsG0580029563.01.T01:CDS |
| AAATTGAAAACACGTGATTT+TGG | 0.185682 | 5:-98621349 | None:intergenic |
| AACAGATTGAGTGCAATTTC+AGG | 0.273035 | 5:-98620203 | None:intergenic |
| AAGACGTTGAGATAAACTAG+TGG | 0.706365 | 5:-98621503 | None:intergenic |
| AAGACTAGAAGATAGTTATT+TGG | 0.317604 | 5:+98621144 | MsG0580029563.01.T01:CDS |
| AAGATAGTTATTTGGTGCAC+GGG | 0.468356 | 5:+98621152 | MsG0580029563.01.T01:CDS |
| AAGCTTGGTTCATATCATAT+TGG | 0.278633 | 5:-98622062 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGCTTGGTTCATATCATAT+TGG | - | Chr5:98622065-98622084 | None:intergenic | 30.0% | |
| AAGCTTGTGAGTTGTTTAAT+AGG | + | Chr5:98622079-98622098 | MsG0580029563.01.T01:CDS | 30.0% | |
| AATCTGTTTGAACATGCTTA+TGG | + | Chr5:98620563-98620582 | MsG0580029563.01.T01:CDS | 30.0% | |
| ACTTATAATATCATGGTACG+TGG | + | Chr5:98622135-98622154 | MsG0580029563.01.T01:CDS | 30.0% | |
| AGCTTGGTTCATATCATATT+GGG | - | Chr5:98622064-98622083 | None:intergenic | 30.0% | |
| ATTGAGTATCTTGAAACGTT+TGG | + | Chr5:98620110-98620129 | MsG0580029563.01.T01:CDS | 30.0% | |
| GAGGCAGAAAATTATCTTAT+CGG | + | Chr5:98620416-98620435 | MsG0580029563.01.T01:CDS | 30.0% | |
| GCTTATGGTGATTGTATAAA+GGG | + | Chr5:98620578-98620597 | MsG0580029563.01.T01:CDS | 30.0% | |
| GTTTAATAGGATACGTCATA+GGG | + | Chr5:98622092-98622111 | MsG0580029563.01.T01:CDS | 30.0% | |
| TACTTGTAAGCCAAGTTATT+CGG | - | Chr5:98620887-98620906 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 98620098 | 98622287 | 98620098 | ID=MsG0580029563.01;Name=MsG0580029563.01 |
| Chr5 | mRNA | 98620098 | 98622287 | 98620098 | ID=MsG0580029563.01.T01;Parent=MsG0580029563.01;Name=MsG0580029563.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|729 |
| Chr5 | exon | 98620098 | 98622287 | 98620098 | ID=MsG0580029563.01.T01:exon:25801;Parent=MsG0580029563.01.T01 |
| Chr5 | CDS | 98620098 | 98622287 | 98620098 | ID=MsG0580029563.01.T01:cds;Parent=MsG0580029563.01.T01 |
| Gene Sequence |
>MsG0580029563.01.T01 ATGCCGATGGTTATTGAGTATCTTGAAACGTTTGGGAAGCGTGTTTTTGATCATAAAGTTCGTTTTATTGATTTGCTTGTTGTTGGTTATGCAATTGCTGGTAAACCTGAAATTGCACTCAATCTGTTTGGTAAAATGCGTTATCAAGGTCTTGATTTGGATATAGTTGGTTATCATATTTTACTTGGTGCTCTTGCTAAGGATAATTATTTTAATTCTTTTGATGTGATTCTTAATCAGATTCGGAGGAAAGGGTATGCAACGCGTGTTACTGATACTATTGTTGTTAAGTTTCTGTGTAACCAGGGTAGGTTGGATGAGGCAGAAAATTATCTTATCGGTTTGTTGGATAGTGGGAAAAAGTTGCATGGTTCTGAGGTAGGTTTTCTTGTTGGTTCGTTGTGTGAGAGGAAAAAGGTTGGGCATGCTGTTGAGTTGGTTAAGGAGTTTGGGAACTCGGGGTTGAATCTGTTTGAACATGCTTATGGTGATTGTATAAAGGGTCTTGTGAAGGGTGGTAGGTTGGATGAGGCTTTGGAGTTTTTTAGGCAGAAGAGAGATTCTGAAGGGTATGTTCCTCGTCGGTATAGGTATAACTTGTTGATTTGTAGGCTTTTGAGGGAGAGTAGGCTTCACGAGGTATATGATTTGTTGATGGATATGCAGGAGTCTTGTATTCCACCTGATATGGTTACTATGAGTGCTGTCGTGTGCTTCCTTTGCAAAGCTGGGATGGTGAATGTTGCACTCCAATTGTACGAATCCAGGTCTCAGTTTGGACTGAATCCGAATAACTTGGCTTACAAGTATTTGATACTTAATCTCTGTTGGGATGGGAGTGTTAAGGAAGCGTACAGTGTTTTCAAAAATTTTATCGGAAATGGTCTTTTTCCTGATACACAAACCTTTACCACACTTGCCAATGCTTTGTGCAGAGAGTGCAAGGTTGATGAGATGAAGGAGCTGATGCATCTTGCCTGGGAGCGAAACTTTACACCGAGCCAAATCACATATGACAAGTTTATATTGGCTTTGTGCCAAGCTGGAAGACTAGAAGATAGTTATTTGGTGCACGGGGATCACAACAATGCAACTGCTAGACTGTCCTATGGTAGGATGATTAAGAGTTTTATTAAGTTTAAGAGGGGAGACATTGCTGCTCGCCTTCTGGTTGAAATGAAAGAGAAGAATTTTAAATTGACGCGGATCTGGTGCACCGCCGTTATTGGAAGTTTACTTGATATGGAAAATCCAAAATCACGTGTTTTCAATTTATTGGACAGGCTGACTCATGGTAAACCTGATACTAAGATTTTCAACTTTTTCATTGATGGAGCTGGGCATGCCAATAATACAGAGTTGGCCAGGGAAGTATATGAGTTGATGCTAAGGAATAACATTGCGCCCACTAGTTTATCTCAACGTCTTGTGTTGAATGGTTATTTGAAAAGTGGAAAGATATATGATGCTTTGAATTTCTTTCACAGTCTTCGGTGCCAGGGCACAGTGAGCAAAAAAGTATATCAATCCATTATTTTTGCTCTCTGCAAATCCAGTAAGGTTGACATTGCACACAATTTCTTGTTTCAAATGTTAAAAGCTGGATTAAATCCAAGCATTGAATGCTTTGAGATTCTTGTGCAGACGCTTTGCTCATTGGAAAGATACCATGAAGCGATAAATCTTGTTCATGTGTATATAAAAATGGGACGTCGATTAACTAATTTTCTTGGCAACATACTTCTTTCTCATTCTTTGATATCGCCGGATATCTATCATGCATGTGTTCGTTTAAGAGGAGCCAAAGAGGAAGAATGTTCTCCTATGTCTACACTGAGCTTTGTCATTGGTGCATTTTCTCGTTGTCTTAGAGTAAATCCTTCTGTTGAGGAATTGGAGAAACTAATATCAATGTGCTTTCCGCTTGACCTTTACACCTACAATCAGCTGCTGAGAAGAGTAACCCAATATGATATGAACCAAGCTTGTGAGTTGTTTAATAGGATACGTCATAGGGGTTATGAGCCTAATGATTGGACTTATAATATCATGGTACGTGGTTTCTCAAATCACGGGAGAAATGATGAGGCTAAGCAGTGGGTCGAAGAAATGCACCAAAAAGGATTTTATCCAAGAGAGAATACTAAAAGATGTGTACAAAAAGGGATTTTATCCAACAGAGAATACTAG |
| Protein sequence |
>MsG0580029563.01.T01 MPMVIEYLETFGKRVFDHKVRFIDLLVVGYAIAGKPEIALNLFGKMRYQGLDLDIVGYHILLGALAKDNYFNSFDVILNQIRRKGYATRVTDTIVVKFLCNQGRLDEAENYLIGLLDSGKKLHGSEVGFLVGSLCERKKVGHAVELVKEFGNSGLNLFEHAYGDCIKGLVKGGRLDEALEFFRQKRDSEGYVPRRYRYNLLICRLLRESRLHEVYDLLMDMQESCIPPDMVTMSAVVCFLCKAGMVNVALQLYESRSQFGLNPNNLAYKYLILNLCWDGSVKEAYSVFKNFIGNGLFPDTQTFTTLANALCRECKVDEMKELMHLAWERNFTPSQITYDKFILALCQAGRLEDSYLVHGDHNNATARLSYGRMIKSFIKFKRGDIAARLLVEMKEKNFKLTRIWCTAVIGSLLDMENPKSRVFNLLDRLTHGKPDTKIFNFFIDGAGHANNTELAREVYELMLRNNIAPTSLSQRLVLNGYLKSGKIYDALNFFHSLRCQGTVSKKVYQSIIFALCKSSKVDIAHNFLFQMLKAGLNPSIECFEILVQTLCSLERYHEAINLVHVYIKMGRRLTNFLGNILLSHSLISPDIYHACVRLRGAKEEECSPMSTLSFVIGAFSRCLRVNPSVEELEKLISMCFPLDLYTYNQLLRRVTQYDMNQACELFNRIRHRGYEPNDWTYNIMVRGFSNHGRNDEAKQWVEEMHQKGFYPRENTKRCVQKGILSNREY* |
