AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005664.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005664.01.T01 MTR_1g105470 98.876 178 2 0 1 178 166 343 9.84e-127 360
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005664.01.T01 AT1G61790 68.362 177 56 0 1 177 169 345 1.15e-91 271
MsG0180005664.01.T01 AT1G61790 68.362 177 56 0 1 177 169 345 1.15e-91 271
MsG0180005664.01.T01 AT1G11560 60.452 177 70 0 1 177 165 341 1.14e-71 220

Find 30 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATGGGATTCATGGGTTT+TGG 0.245577 1:-94696315 MsG0180005664.01.T01:CDS
GATCCTAGGGTTTGGTTGGC+TGG 0.275919 1:-94696652 MsG0180005664.01.T01:CDS
GATAATTGGAAGACTGGTTA+TGG 0.283602 1:-94696331 MsG0180005664.01.T01:CDS
GAAAATGCCTATGTTTCTTC+AGG 0.313592 1:-94696575 MsG0180005664.01.T01:CDS
GTGCAATGCATAACATTATA+AGG 0.321034 1:-94696597 MsG0180005664.01.T01:CDS
TACACTTGTGGGTTTGCTTT+TGG 0.330345 1:-94696464 MsG0180005664.01.T01:CDS
AAGACTGGTTATGGGATTCA+TGG 0.386907 1:-94696322 MsG0180005664.01.T01:CDS
ATAATTGGAAGACTGGTTAT+GGG 0.413547 1:-94696330 MsG0180005664.01.T01:CDS
AAACATTGTTGCATGATCCT+AGG 0.420052 1:-94696666 MsG0180005664.01.T01:CDS
AAAATCGGAGGACGATGAAT+TGG 0.423321 1:+94696769 None:intergenic
AAAATTCAGTTCCAGCTAGA+AGG 0.427617 1:+94696292 None:intergenic
GGATTCTTGTATACACTTGT+GGG 0.454272 1:-94696475 MsG0180005664.01.T01:CDS
TGGATTCTTGTATACACTTG+TGG 0.458267 1:-94696476 MsG0180005664.01.T01:CDS
TGTTGCATGATCCTAGGGTT+TGG 0.462490 1:-94696660 MsG0180005664.01.T01:CDS
AATTATTCTCATTGTTGTTG+TGG 0.473492 1:-94696734 MsG0180005664.01.T01:CDS
ATCATAACCACCCTCTGCAC+TGG 0.480009 1:+94696403 None:intergenic
AACATTGTTGCATGATCCTA+GGG 0.482379 1:-94696665 MsG0180005664.01.T01:CDS
GCATGATCCTAGGGTTTGGT+TGG 0.487069 1:-94696656 MsG0180005664.01.T01:CDS
TGGAATGCAGCTTGGTGCTG+AGG 0.505373 1:-94696509 MsG0180005664.01.T01:CDS
AGACTGGTTATGGGATTCAT+GGG 0.517745 1:-94696321 MsG0180005664.01.T01:CDS
GGAATGCAGCTTGGTGCTGA+GGG 0.518582 1:-94696508 MsG0180005664.01.T01:CDS
CAGGGATCTGGAATGCAGCT+TGG 0.525185 1:-94696517 MsG0180005664.01.T01:CDS
CAAGAGTGTTCCAGTGCAGA+GGG 0.538565 1:-94696413 MsG0180005664.01.T01:CDS
TCAAGAGTGTTCCAGTGCAG+AGG 0.559711 1:-94696414 MsG0180005664.01.T01:CDS
TTACGATCCTGAAGAAACAT+AGG 0.564980 1:+94696568 None:intergenic
GAACCAGCCAACCAAACCCT+AGG 0.603893 1:+94696649 None:intergenic
GCGAAAACGAAACTCTCTGT+GGG 0.617553 1:-94696793 MsG0180005664.01.T01:CDS
AGCGAAAACGAAACTCTCTG+TGG 0.639306 1:-94696794 MsG0180005664.01.T01:CDS
TTGTTTCTAGACAAAATCGG+AGG 0.672104 1:+94696757 None:intergenic
GAGTGTTCCAGTGCAGAGGG+TGG 0.685915 1:-94696410 MsG0180005664.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTTTCTAGACAAAAT+CGG + Chr1:94696357-94696376 None:intergenic 20.0%
!! AATTATTCTCATTGTTGTTG+TGG - Chr1:94696374-94696393 MsG0180005664.01.T01:CDS 25.0%
GTGCAATGCATAACATTATA+AGG - Chr1:94696511-94696530 MsG0180005664.01.T01:CDS 30.0%
! AAGCTTGTGTTTTTCTATCA+GGG - Chr1:94696573-94696592 MsG0180005664.01.T01:CDS 30.0%
! ATAATTGGAAGACTGGTTAT+GGG - Chr1:94696778-94696797 MsG0180005664.01.T01:CDS 30.0%
! GGTTATGATTTTTGCTCTTT+TGG - Chr1:94696719-94696738 MsG0180005664.01.T01:CDS 30.0%
! TTTGTTAAGAAGCTTTTGAC+TGG - Chr1:94696417-94696436 MsG0180005664.01.T01:CDS 30.0%
! TTTTTGGATAATTGGAAGAC+TGG - Chr1:94696771-94696790 MsG0180005664.01.T01:CDS 30.0%
!!! AGGTTGTGTTTTTGGATAAT+TGG - Chr1:94696763-94696782 MsG0180005664.01.T01:CDS 30.0%
!!! GTTTACTTTTTCAGTGTTTC+TGG - Chr1:94696489-94696508 MsG0180005664.01.T01:CDS 30.0%
AAACATTGTTGCATGATCCT+AGG - Chr1:94696442-94696461 MsG0180005664.01.T01:CDS 35.0%
AACATTGTTGCATGATCCTA+GGG - Chr1:94696443-94696462 MsG0180005664.01.T01:CDS 35.0%
GAAAATGCCTATGTTTCTTC+AGG - Chr1:94696533-94696552 MsG0180005664.01.T01:CDS 35.0%
GGATTCTTGTATACACTTGT+GGG - Chr1:94696633-94696652 MsG0180005664.01.T01:CDS 35.0%
TAGAAAAACACAAGCTTCGA+AGG + Chr1:94696570-94696589 None:intergenic 35.0%
TGGATTCTTGTATACACTTG+TGG - Chr1:94696632-94696651 MsG0180005664.01.T01:CDS 35.0%
TTACGATCCTGAAGAAACAT+AGG + Chr1:94696543-94696562 None:intergenic 35.0%
! GAAGCTTGTGTTTTTCTATC+AGG - Chr1:94696572-94696591 MsG0180005664.01.T01:CDS 35.0%
! GATAATTGGAAGACTGGTTA+TGG - Chr1:94696777-94696796 MsG0180005664.01.T01:CDS 35.0%
!! TTGTTTCTAGACAAAATCGG+AGG + Chr1:94696354-94696373 None:intergenic 35.0%
!!! TGCTCTTTTGGTTTGTTTTC+TGG - Chr1:94696731-94696750 MsG0180005664.01.T01:CDS 35.0%
!!! TTGTTGTTGTGGTTTTGATC+TGG - Chr1:94696385-94696404 MsG0180005664.01.T01:CDS 35.0%
AAAATCGGAGGACGATGAAT+TGG + Chr1:94696342-94696361 None:intergenic 40.0%
! AAGACTGGTTATGGGATTCA+TGG - Chr1:94696786-94696805 MsG0180005664.01.T01:CDS 40.0%
! AGACTGGTTATGGGATTCAT+GGG - Chr1:94696787-94696806 MsG0180005664.01.T01:CDS 40.0%
! CTTTTGGCGTTTATGACTCA+TGG - Chr1:94696660-94696679 MsG0180005664.01.T01:CDS 40.0%
! GTGTTTTTCTATCAGGGATC+TGG - Chr1:94696579-94696598 MsG0180005664.01.T01:CDS 40.0%
! GTTATGGGATTCATGGGTTT+TGG - Chr1:94696793-94696812 MsG0180005664.01.T01:CDS 40.0%
! TGTGAAGCAGGTTGTGTTTT+TGG - Chr1:94696755-94696774 MsG0180005664.01.T01:CDS 40.0%
!! TACACTTGTGGGTTTGCTTT+TGG - Chr1:94696644-94696663 MsG0180005664.01.T01:CDS 40.0%
AGCGAAAACGAAACTCTCTG+TGG - Chr1:94696314-94696333 MsG0180005664.01.T01:CDS 45.0%
GCGAAAACGAAACTCTCTGT+GGG - Chr1:94696315-94696334 MsG0180005664.01.T01:CDS 45.0%
TGTTGCATGATCCTAGGGTT+TGG - Chr1:94696448-94696467 MsG0180005664.01.T01:CDS 45.0%
!!! TTGTTTTCTGGCTGTGAAGC+AGG - Chr1:94696743-94696762 MsG0180005664.01.T01:CDS 45.0%
ATCATAACCACCCTCTGCAC+TGG + Chr1:94696708-94696727 None:intergenic 50.0%
! GCATGATCCTAGGGTTTGGT+TGG - Chr1:94696452-94696471 MsG0180005664.01.T01:CDS 50.0%
!! ATGGGTTTTGGCCTTCTAGC+TGG - Chr1:94696805-94696824 MsG0180005664.01.T01:CDS 50.0%
!! CAAGAGTGTTCCAGTGCAGA+GGG - Chr1:94696695-94696714 MsG0180005664.01.T01:CDS 50.0%
!! GGTGCTGAGGGTTTTACTGT+TGG - Chr1:94696612-94696631 MsG0180005664.01.T01:CDS 50.0%
!! TCAAGAGTGTTCCAGTGCAG+AGG - Chr1:94696694-94696713 MsG0180005664.01.T01:CDS 50.0%
GAACCAGCCAACCAAACCCT+AGG + Chr1:94696462-94696481 None:intergenic 55.0%
! CAGGGATCTGGAATGCAGCT+TGG - Chr1:94696591-94696610 MsG0180005664.01.T01:CDS 55.0%
! GATCCTAGGGTTTGGTTGGC+TGG - Chr1:94696456-94696475 MsG0180005664.01.T01:CDS 55.0%
!! GGAATGCAGCTTGGTGCTGA+GGG - Chr1:94696600-94696619 MsG0180005664.01.T01:CDS 55.0%
!! TGGAATGCAGCTTGGTGCTG+AGG - Chr1:94696599-94696618 MsG0180005664.01.T01:CDS 55.0%
GAGTGTTCCAGTGCAGAGGG+TGG - Chr1:94696698-94696717 MsG0180005664.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 94696297 94696833 94696297 ID=MsG0180005664.01;Name=MsG0180005664.01
Chr1 mRNA 94696297 94696833 94696297 ID=MsG0180005664.01.T01;Parent=MsG0180005664.01;Name=MsG0180005664.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|178
Chr1 exon 94696297 94696833 94696297 ID=MsG0180005664.01.T01:exon:50194;Parent=MsG0180005664.01.T01
Chr1 CDS 94696297 94696833 94696297 ID=MsG0180005664.01.T01:cds;Parent=MsG0180005664.01.T01
Gene Sequence

>MsG0180005664.01.T01

ATGGCTGAATTCGTCGAAGCGAAAACGAAACTCTCTGTGGGACCAATTCATCGTCCTCCGATTTTGTCTAGAAACAAAATTATTCTCATTGTTGTTGTGGTTTTGATCTGGTTGTCGTTTTTTGTTAAGAAGCTTTTGACTGGAAAAACATTGTTGCATGATCCTAGGGTTTGGTTGGCTGGTTCTGTTTTTGTTTACTTTTTCAGTGTTTCTGGTGCAATGCATAACATTATAAGGAAAATGCCTATGTTTCTTCAGGATCGTAATGATCCTTCGAAGCTTGTGTTTTTCTATCAGGGATCTGGAATGCAGCTTGGTGCTGAGGGTTTTACTGTTGGATTCTTGTATACACTTGTGGGTTTGCTTTTGGCGTTTATGACTCATGGTCTTGTTAAGATCAAGAGTGTTCCAGTGCAGAGGGTGGTTATGATTTTTGCTCTTTTGGTTTGTTTTCTGGCTGTGAAGCAGGTTGTGTTTTTGGATAATTGGAAGACTGGTTATGGGATTCATGGGTTTTGGCCTTCTAGCTGGAACTGA

Protein sequence

>MsG0180005664.01.T01

MAEFVEAKTKLSVGPIHRPPILSRNKIILIVVVVLIWLSFFVKKLLTGKTLLHDPRVWLAGSVFVYFFSVSGAMHNIIRKMPMFLQDRNDPSKLVFFYQGSGMQLGAEGFTVGFLYTLVGLLLAFMTHGLVKIKSVPVQRVVMIFALLVCFLAVKQVVFLDNWKTGYGIHGFWPSSWN*