AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000142.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000142.01.T01 MTR_1g022360 96.040 101 4 0 1 101 21 121 2.21e-66 203
MsG0180000142.01.T01 MTR_7g075010 45.455 99 54 0 2 100 191 289 4.96e-23 92.0
MsG0180000142.01.T01 MTR_4g068360 35.106 94 61 0 2 95 507 600 1.30e-16 74.3
MsG0180000142.01.T01 MTR_8g105170 34.409 93 61 0 4 96 464 556 7.82e-13 63.5
MsG0180000142.01.T01 MTR_7g091470 32.979 94 63 0 2 95 577 670 9.47e-13 63.5
MsG0180000142.01.T01 MTR_1g112240 30.693 101 70 0 2 102 287 387 1.16e-12 63.2
MsG0180000142.01.T01 MTR_5g008300 28.000 100 72 0 1 100 938 1037 1.96e-12 62.4
MsG0180000142.01.T01 MTR_1g068960 30.851 94 65 0 2 95 247 340 3.96e-12 61.6
MsG0180000142.01.T01 MTR_2g036560 24.545 110 76 1 1 110 667 769 2.59e-11 59.3
MsG0180000142.01.T01 MTR_5g090210 30.337 89 62 0 8 96 464 552 3.32e-11 58.9
MsG0180000142.01.T01 MTR_7g007400 27.083 96 70 0 2 97 296 391 4.48e-11 58.5
MsG0180000142.01.T01 MTR_0014s0250 31.522 92 63 0 4 95 420 511 5.78e-11 58.2
MsG0180000142.01.T01 MTR_3g115270 32.222 90 58 1 7 96 509 595 5.92e-11 58.2
MsG0180000142.01.T01 MTR_7g405940 26.042 96 71 0 2 97 314 409 9.96e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000142.01.T01 AT1G19520 49.505 101 51 0 1 101 393 493 6.26e-32 118
MsG0180000142.01.T01 AT1G01970 42.424 99 57 0 2 100 175 273 1.97e-19 82.0
MsG0180000142.01.T01 AT2G35130 30.000 110 77 0 2 111 331 440 2.56e-14 67.8
MsG0180000142.01.T01 AT2G35130 30.000 110 77 0 2 111 306 415 3.58e-14 67.4
MsG0180000142.01.T01 AT2G35130 30.000 110 77 0 2 111 309 418 3.58e-14 67.4
MsG0180000142.01.T01 AT3G04130 29.630 108 74 2 5 111 307 413 2.02e-13 65.5
MsG0180000142.01.T01 AT3G04130 29.630 108 74 2 5 111 307 413 2.02e-13 65.5
MsG0180000142.01.T01 AT5G61990 27.174 92 67 0 4 95 604 695 4.63e-13 64.3
MsG0180000142.01.T01 AT1G62720 34.043 94 62 0 2 95 149 242 1.24e-11 60.1
MsG0180000142.01.T01 AT5G42310 30.526 95 66 0 2 96 524 618 1.98e-11 59.7
MsG0180000142.01.T01 AT3G18110 28.000 100 72 0 1 100 901 1000 3.11e-11 59.3
MsG0180000142.01.T01 AT5G21222 27.619 105 73 1 6 110 580 681 3.80e-11 58.9
MsG0180000142.01.T01 AT5G21222 27.619 105 73 1 6 110 580 681 3.80e-11 58.9
MsG0180000142.01.T01 AT5G21222 27.619 105 73 1 6 110 580 681 3.80e-11 58.9
MsG0180000142.01.T01 AT5G21222 27.619 105 73 1 6 110 580 681 3.80e-11 58.9
MsG0180000142.01.T01 AT1G13800 29.032 93 66 0 3 95 576 668 6.21e-11 58.2
MsG0180000142.01.T01 AT1G13800 29.032 93 66 0 3 95 576 668 6.21e-11 58.2

Find 25 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAGAAGATGAATGAAAA+TGG 0.299964 1:-1987509 MsG0180000142.01.T01:CDS
ATCAGTGTTTCACCCTTTGT+AGG 0.323330 1:+1987320 None:intergenic
ATGACATGTATATCCTTTGT+AGG 0.353024 1:+1987269 None:intergenic
AGGTTCACCAGCATTGATAT+AGG 0.408715 1:+1987340 None:intergenic
ATTTAAAATCTTGAGTAGCC+TGG 0.418541 1:-1987405 MsG0180000142.01.T01:CDS
CGCCACGTTGAGAATAATAT+CGG 0.469885 1:+1987242 None:intergenic
GAGTTGTAGACTCTCGTGTC+TGG 0.475797 1:+1987374 None:intergenic
GTCCACATGTACAGCAAGAT+AGG 0.481832 1:-1987452 MsG0180000142.01.T01:CDS
TTTAAAATCTTGAGTAGCCT+GGG 0.503901 1:-1987404 MsG0180000142.01.T01:CDS
ACATTGGCTATCGAAGCATC+TGG 0.528199 1:+1987479 None:intergenic
CAATGCTGGTGAACCTACAA+AGG 0.532330 1:-1987333 MsG0180000142.01.T01:CDS
TCGTGTCTGGTTGGAAACCC+AGG 0.536262 1:+1987387 None:intergenic
AGTCTACAACTCAATGATCA+TGG 0.557154 1:-1987363 MsG0180000142.01.T01:CDS
ATCATGGCCTATATCAATGC+TGG 0.564676 1:-1987347 MsG0180000142.01.T01:CDS
TGTAGACTCTCGTGTCTGGT+TGG 0.572645 1:+1987378 None:intergenic
TACCTATCTTGCTGTACATG+TGG 0.574085 1:+1987450 None:intergenic
TGAAACACTGATGAGGCAGA+TGG 0.584225 1:-1987309 MsG0180000142.01.T01:CDS
AATGCTGGTGAACCTACAAA+GGG 0.617131 1:-1987332 MsG0180000142.01.T01:CDS
GAGAGATATTAAGCCTACAA+AGG 0.621763 1:-1987282 MsG0180000142.01.T01:CDS
TGCTTCGATAGCCAATGTGA+TGG 0.640106 1:-1987474 MsG0180000142.01.T01:CDS
CTCCGATATTATTCTCAACG+TGG 0.648716 1:-1987244 MsG0180000142.01.T01:CDS
CAAAGGGTGAAACACTGATG+AGG 0.685674 1:-1987316 MsG0180000142.01.T01:CDS
GTACATGTGGACCATCACAT+TGG 0.702814 1:+1987463 None:intergenic
TCCTCTCAGTATCATCAATG+CGG 0.705522 1:+1987534 None:intergenic
ACCGCATTGATGATACTGAG+AGG 0.716042 1:-1987535 MsG0180000142.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTAAGAAGATGAATGAAAA+TGG - Chr1:1987277-1987296 MsG0180000142.01.T01:CDS 25.0%
ATGACATGTATATCCTTTGT+AGG + Chr1:1987520-1987539 None:intergenic 30.0%
! ATTTAAAATCTTGAGTAGCC+TGG - Chr1:1987381-1987400 MsG0180000142.01.T01:CDS 30.0%
! TTTAAAATCTTGAGTAGCCT+GGG - Chr1:1987382-1987401 MsG0180000142.01.T01:CDS 30.0%
AGTCTACAACTCAATGATCA+TGG - Chr1:1987423-1987442 MsG0180000142.01.T01:CDS 35.0%
GAGAGATATTAAGCCTACAA+AGG - Chr1:1987504-1987523 MsG0180000142.01.T01:CDS 35.0%
AATGCTGGTGAACCTACAAA+GGG - Chr1:1987454-1987473 MsG0180000142.01.T01:CDS 40.0%
ATCAGTGTTTCACCCTTTGT+AGG + Chr1:1987469-1987488 None:intergenic 40.0%
ATCATGGCCTATATCAATGC+TGG - Chr1:1987439-1987458 MsG0180000142.01.T01:CDS 40.0%
CGCCACGTTGAGAATAATAT+CGG + Chr1:1987547-1987566 None:intergenic 40.0%
CTCCGATATTATTCTCAACG+TGG - Chr1:1987542-1987561 MsG0180000142.01.T01:CDS 40.0%
TCCTCTCAGTATCATCAATG+CGG + Chr1:1987255-1987274 None:intergenic 40.0%
! TACCTATCTTGCTGTACATG+TGG + Chr1:1987339-1987358 None:intergenic 40.0%
!! AGGTTCACCAGCATTGATAT+AGG + Chr1:1987449-1987468 None:intergenic 40.0%
CAAAGGGTGAAACACTGATG+AGG - Chr1:1987470-1987489 MsG0180000142.01.T01:CDS 45.0%
CAATGCTGGTGAACCTACAA+AGG - Chr1:1987453-1987472 MsG0180000142.01.T01:CDS 45.0%
GTACATGTGGACCATCACAT+TGG + Chr1:1987326-1987345 None:intergenic 45.0%
GTCCACATGTACAGCAAGAT+AGG - Chr1:1987334-1987353 MsG0180000142.01.T01:CDS 45.0%
TGAAACACTGATGAGGCAGA+TGG - Chr1:1987477-1987496 MsG0180000142.01.T01:CDS 45.0%
TGCTTCGATAGCCAATGTGA+TGG - Chr1:1987312-1987331 MsG0180000142.01.T01:CDS 45.0%
! ACATTGGCTATCGAAGCATC+TGG + Chr1:1987310-1987329 None:intergenic 45.0%
! ACCGCATTGATGATACTGAG+AGG - Chr1:1987251-1987270 MsG0180000142.01.T01:CDS 45.0%
GAGTTGTAGACTCTCGTGTC+TGG + Chr1:1987415-1987434 None:intergenic 50.0%
TGTAGACTCTCGTGTCTGGT+TGG + Chr1:1987411-1987430 None:intergenic 50.0%
TCGTGTCTGGTTGGAAACCC+AGG + Chr1:1987402-1987421 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 1987235 1987573 1987235 ID=MsG0180000142.01;Name=MsG0180000142.01
Chr1 mRNA 1987235 1987573 1987235 ID=MsG0180000142.01.T01;Parent=MsG0180000142.01;Name=MsG0180000142.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|112
Chr1 exon 1987235 1987573 1987235 ID=MsG0180000142.01.T01:exon:2526;Parent=MsG0180000142.01.T01
Chr1 CDS 1987235 1987573 1987235 ID=MsG0180000142.01.T01:cds;Parent=MsG0180000142.01.T01
Gene Sequence

>MsG0180000142.01.T01

ATGTATGCTAAAGAGAACCGCATTGATGATACTGAGAGGATGCTTAAGAAGATGAATGAAAATGGTTTACAACCAGATGCTTCGATAGCCAATGTGATGGTCCACATGTACAGCAAGATAGGTAATCTTGAACGTGCAGAAGAAGCATTTAAAATCTTGAGTAGCCTGGGTTTCCAACCAGACACGAGAGTCTACAACTCAATGATCATGGCCTATATCAATGCTGGTGAACCTACAAAGGGTGAAACACTGATGAGGCAGATGGAGATGAGAGATATTAAGCCTACAAAGGATATACATGTCATTGCTCCGATATTATTCTCAACGTGGCGACATTGA

Protein sequence

>MsG0180000142.01.T01

MYAKENRIDDTERMLKKMNENGLQPDASIANVMVHMYSKIGNLERAEEAFKILSSLGFQPDTRVYNSMIMAYINAGEPTKGETLMRQMEMRDIKPTKDIHVIAPILFSTWRH*