Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029782.01.T01 | AET00479.1 | 67.033 | 273 | 21 | 4 | 312 | 554 | 11 | 244 | 6.57E-111 | 343 |
| MsG0580029782.01.T01 | AET00479.1 | 38.115 | 244 | 133 | 3 | 215 | 446 | 60 | 297 | 1.74E-37 | 151 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048036.01 | MsG0580029782.01 | 0.832167 | 1.103109e-55 | 1.223020e-52 |
| MsG0080048345.01 | MsG0580029782.01 | 0.813862 | 2.064687e-51 | 1.363908e-48 |
| MsG0080048347.01 | MsG0580029782.01 | 0.847502 | 1.110200e-59 | 1.977410e-56 |
| MsG0080048349.01 | MsG0580029782.01 | 0.808984 | 2.366997e-50 | 1.372937e-47 |
| MsG0080048352.01 | MsG0580029782.01 | 0.848768 | 4.964873e-60 | 9.209886e-57 |
| MsG0080049066.01 | MsG0580029782.01 | -0.812560 | 3.986781e-51 | 2.543071e-48 |
| MsG0180000930.01 | MsG0580029782.01 | 0.813529 | 2.443737e-51 | 1.599893e-48 |
| MsG0180001072.01 | MsG0580029782.01 | -0.817521 | 3.158023e-52 | 2.305123e-49 |
| MsG0180001533.01 | MsG0580029782.01 | 0.816946 | 4.253858e-52 | 3.056675e-49 |
| MsG0180002935.01 | MsG0580029782.01 | 0.815220 | 1.033705e-51 | 7.083864e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029782.01.T01 | MTR_5g092520 | 67.033 | 273 | 21 | 4 | 312 | 554 | 11 | 244 | 7.96e-115 | 343 |
| MsG0580029782.01.T01 | MTR_5g092520 | 38.115 | 244 | 133 | 3 | 215 | 446 | 60 | 297 | 2.11e-41 | 151 |
| MsG0580029782.01.T01 | MTR_5g092520 | 42.000 | 100 | 34 | 2 | 205 | 304 | 217 | 292 | 8.81e-12 | 66.6 |
| MsG0580029782.01.T01 | MTR_5g092630 | 65.660 | 265 | 54 | 3 | 306 | 534 | 8 | 271 | 1.64e-113 | 341 |
| MsG0580029782.01.T01 | MTR_5g092630 | 50.476 | 210 | 93 | 4 | 205 | 410 | 83 | 285 | 7.69e-52 | 181 |
| MsG0580029782.01.T01 | MTR_5g092630 | 52.000 | 75 | 36 | 0 | 205 | 279 | 253 | 327 | 6.67e-18 | 85.9 |
| MsG0580029782.01.T01 | MTR_5g092630 | 65.660 | 265 | 54 | 3 | 306 | 534 | 8 | 271 | 1.94e-113 | 342 |
| MsG0580029782.01.T01 | MTR_5g092630 | 43.750 | 272 | 111 | 6 | 205 | 441 | 83 | 347 | 1.48e-52 | 183 |
| MsG0580029782.01.T01 | MTR_5g092510 | 66.122 | 245 | 58 | 5 | 313 | 534 | 11 | 253 | 3.01e-101 | 309 |
| MsG0580029782.01.T01 | MTR_5g092510 | 49.515 | 206 | 85 | 3 | 205 | 410 | 81 | 267 | 1.48e-50 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029782.01.T01 | AT1G27180 | 33.091 | 275 | 126 | 12 | 306 | 537 | 8 | 267 | 7.52e-28 | 119 |
| MsG0580029782.01.T01 | AT1G27180 | 32.500 | 280 | 147 | 9 | 148 | 411 | 22 | 275 | 6.87e-26 | 113 |
| MsG0580029782.01.T01 | AT1G72930 | 49.425 | 87 | 43 | 1 | 445 | 531 | 4 | 89 | 2.82e-20 | 88.2 |
| MsG0580029782.01.T01 | AT1G72930 | 40.952 | 105 | 62 | 0 | 307 | 411 | 3 | 107 | 8.47e-18 | 80.9 |
| MsG0580029782.01.T01 | AT1G72930 | 44.737 | 76 | 40 | 1 | 205 | 280 | 74 | 147 | 7.80e-12 | 63.9 |
| MsG0580029782.01.T01 | AT1G72950 | 53.488 | 86 | 39 | 1 | 446 | 531 | 7 | 91 | 2.94e-20 | 93.2 |
| MsG0580029782.01.T01 | AT1G72950 | 40.952 | 105 | 62 | 0 | 307 | 411 | 5 | 109 | 2.43e-17 | 84.7 |
| MsG0580029782.01.T01 | AT1G72950 | 44.304 | 79 | 42 | 1 | 202 | 280 | 73 | 149 | 1.34e-12 | 70.1 |
| MsG0580029782.01.T01 | AT1G72930 | 49.425 | 87 | 43 | 1 | 445 | 531 | 4 | 89 | 2.95e-20 | 89.0 |
| MsG0580029782.01.T01 | AT1G72930 | 42.202 | 109 | 55 | 2 | 307 | 411 | 3 | 107 | 1.49e-17 | 81.3 |
Find 79 sgRNAs with CRISPR-Local
Find 635 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTTTGGATATTCCACA+TGG | 0.695058 | 5:+101965017 | MsG0580029782.01.T01:CDS |
| AAAAGTTGAAGCAATTGATC+GGG | 0.527004 | 5:+101954044 | MsG0580029782.01.T01:CDS |
| AAAATCTTCATGGATGATGA+GGG | 0.581105 | 5:+101964914 | MsG0580029782.01.T01:CDS |
| AAATTGGAAGTCAGCTTTGT+CGG | 0.469386 | 5:+101961101 | MsG0580029782.01.T01:CDS |
| AAGTAAACCAATGGAGGAAT+GGG | 0.436359 | 5:+101954900 | MsG0580029782.01.T01:CDS |
| AAGTAGATCCATCAGACATA+AGG | 0.500366 | 5:+101955245 | MsG0580029782.01.T01:CDS |
| AAGTCTCCTTGATTGCTGGA+TGG | 0.601876 | 5:+101963777 | MsG0580029782.01.T01:CDS |
| AAGTTCATCAAGACAATATG+TGG | 0.539044 | 5:-101960923 | None:intergenic |
| AAGTTCATCAAGACACCATG+TGG | 0.653722 | 5:-101965032 | None:intergenic |
| AAGTTCATCTAGACAACGTG+TGG | 0.621054 | 5:-101963577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAGTTATGGCAAAGACA+TGG | + | Chr5:101955276-101955295 | MsG0580029782.01.T01:CDS | 30.0% | |
| AAAAAGTTATGGTAAAGCCA+TGG | + | Chr5:101956298-101956317 | MsG0580029782.01.T01:intron | 30.0% | |
| AAAAATAGGCCAACAAGTAA+TGG | - | Chr5:101961968-101961987 | None:intergenic | 30.0% | |
| AAAACCTTCATAGATAACGA+CGG | + | Chr5:101960805-101960824 | MsG0580029782.01.T01:intron | 30.0% | |
| AAAACTATGCAAATTCCACA+TGG | + | Chr5:101955149-101955168 | MsG0580029782.01.T01:intron | 30.0% | |
| AAAACTTTGGATATTCCACA+TGG | + | Chr5:101965017-101965036 | MsG0580029782.01.T01:CDS | 30.0% | |
| AAAACTTTGGATATTCGACA+TGG | + | Chr5:101956171-101956190 | MsG0580029782.01.T01:intron | 30.0% | |
| AAAAGACAAAAGAGGGTTAT+AGG | + | Chr5:101963029-101963048 | MsG0580029782.01.T01:intron | 30.0% | |
| AAAAGTTGAAGCAATTGATC+GGG | + | Chr5:101954044-101954063 | MsG0580029782.01.T01:CDS | 30.0% | |
| AAAATCTTCATGGATGATGA+GGG | + | Chr5:101964914-101964933 | MsG0580029782.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 101953994 | 101965156 | 101953994 | ID=MsG0580029782.01;Name=MsG0580029782.01 |
| Chr5 | mRNA | 101953994 | 101965156 | 101953994 | ID=MsG0580029782.01.T01;Parent=MsG0580029782.01;Name=MsG0580029782.01.T01;_AED=0.37;_eAED=0.39;_QI=0|0|0|0.88|1|1|9|0|559 |
| Chr5 | exon | 101953994 | 101954218 | 101953994 | ID=MsG0580029782.01.T01:exon:15050;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101954868 | 101955011 | 101954868 | ID=MsG0580029782.01.T01:exon:15051;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101955208 | 101955399 | 101955208 | ID=MsG0580029782.01.T01:exon:15052;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101957573 | 101957622 | 101957573 | ID=MsG0580029782.01.T01:exon:15053;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101960912 | 101961160 | 101960912 | ID=MsG0580029782.01.T01:exon:15054;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101961622 | 101961749 | 101961622 | ID=MsG0580029782.01.T01:exon:15055;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101963424 | 101963674 | 101963424 | ID=MsG0580029782.01.T01:exon:15056;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101963764 | 101963813 | 101963764 | ID=MsG0580029782.01.T01:exon:15057;Parent=MsG0580029782.01.T01 |
| Chr5 | exon | 101964766 | 101965156 | 101964766 | ID=MsG0580029782.01.T01:exon:15058;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101953994 | 101954218 | 101953994 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101954868 | 101955011 | 101954868 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101955208 | 101955399 | 101955208 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101957573 | 101957622 | 101957573 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101960912 | 101961160 | 101960912 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101961622 | 101961749 | 101961622 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101963424 | 101963674 | 101963424 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101963764 | 101963813 | 101963764 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Chr5 | CDS | 101964766 | 101965156 | 101964766 | ID=MsG0580029782.01.T01:cds;Parent=MsG0580029782.01.T01 |
| Gene Sequence |
>MsG0580029782.01.T01 TTGAAAGACAAAGAAGAGAAACTCATTTCAAATCGTGATCGTGTACGTGCAAAAGTTGAAGCAATTGATCGGGAAACTGAAAAGGTTAGAGATGTTGTGTTTGAGTGGCTAAAAGAAGCAGACATAGTCATGCAAAAAATGGAGAATCTTACATTACAATCAAAACCACCATCATGGATTGAATTTAATAAATTGCAGGAAAAAATTACAGCACTAAATAAGAATGAAAAAAATCATAAGGCAGGGTCTTCCGTAAGAAGTAAACCAATGGAGGAATGGGAAAAATCACTAGACAATTTGAGACATTCAACTGCTCAATGGCAGATGTTTCTCAGTTTAGAGGGGAAGATACTCGCTACTCTTTTACAGAAATGGAATAATCAATTAGTTTGGCCAATCTTTTACAAAGTAGATCCATCAGACATAAGGCATTTGAGAAAAAGTTATGGCAAAGACATGGCTCACAATGAAAAAAATTTGGAATTGACTCGGAGAGAGTACAAAAATGGAAGTCAGCTTTGCTTGAAGTTTCTATTTGTCTGGAATGACTTACACGACTGGGTCATGAGCATGATGGACTTCAAGGCAGTGGCTTTTCCAAGACCTGTGGGGTATGCAGCTTCCACATATTGTCTTGATGAACTTGTCAAGATACTTGAGTGTAGAAACAAGATAAACCAACTGGTTCTCCCAATTTTTTATGAGGTTGATCCTTTTGAGGTAAGAGACCAAACAGGTACATATGGAAAATCTTTTGATGCTCATGAATATAGGTTTGGAAAGGATTATGTGAAGTTGCAAAATTGGAAGTCAGCTTTGTCGGAAGTCGCTGTGTTAACGGATGGCAAATTCCAAAATAGAGAATCCGCTGAGTCCAAATGTGAATATGAATTAATTCAAGACATAGTGAAATGTGTTTCCAACACTTTGCCTCGTTACGATGTTTTTCTTAGTTTCAGCGAACAAGATACCAACTACTCTTTTTCGGGTAGTCTCTATCATGCTTTGCGTAACAAGAGAATCAAGACCTTTTCCGCACATGAAACTGATATTAAGAAACTTCAACCTTCTCCTATTGTGCTCAAGGCACTTAAGGAAGCAAGGATTTCTATTGTTATTCTCTCTGAAAACTTTGCATCTTCCACACGTTGTCTAGATGAACTTGTGAACATCCTTGAATGTATGAAGATGAAAAATCAACTTGTTTGGCCAATCTTTTACAAAGTGGAGCCTTGGGAGAAAGCTTTGCATCAAGTCTCCTTGATTGCTGGATGGAACTACGGAGCTGAGTTCGAATATGAACTCTTCCAGAAGATTGTGGAGTCAACGGTACAATCACTGCCTCGCTACGATGTTTTTCTCAGTTTTTGCGGGGAAGAAGATACTCGCTACTCTTTTACAGGATTTCTCTATCATGATTTGCGTCGTGAGGGATTCAAAATCTTCATGGATGATGAGGGATTGAAGGGTGGAAACCAAATTTCACAAACTTTACTCAAAGCAATTGAAAAATCAAGGCTTTCAATTGTTGTTTTGTCTGAAAACTTTGGATATTCCACATGGTGTCTTGATGAACTTGTCAAGATTATGGAAGCAAGAAGACCAATAATCAATTGGTGTGGCCAATATTTTACAAATAGAACAATCCGATATAAGAAATCAAACAAAAAGTTATGGTAA |
| Protein sequence |
>MsG0580029782.01.T01 LKDKEEKLISNRDRVRAKVEAIDRETEKVRDVVFEWLKEADIVMQKMENLTLQSKPPSWIEFNKLQEKITALNKNEKNHKAGSSVRSKPMEEWEKSLDNLRHSTAQWQMFLSLEGKILATLLQKWNNQLVWPIFYKVDPSDIRHLRKSYGKDMAHNEKNLELTRREYKNGSQLCLKFLFVWNDLHDWVMSMMDFKAVAFPRPVGYAASTYCLDELVKILECRNKINQLVLPIFYEVDPFEVRDQTGTYGKSFDAHEYRFGKDYVKLQNWKSALSEVAVLTDGKFQNRESAESKCEYELIQDIVKCVSNTLPRYDVFLSFSEQDTNYSFSGSLYHALRNKRIKTFSAHETDIKKLQPSPIVLKALKEARISIVILSENFASSTRCLDELVNILECMKMKNQLVWPIFYKVEPWEKALHQVSLIAGWNYGAEFEYELFQKIVESTVQSLPRYDVFLSFCGEEDTRYSFTGFLYHDLRREGFKIFMDDEGLKGGNQISQTLLKAIEKSRLSIVVLSENFGYSTWCLDELVKIMEARRPIINWCGQYFTNRTIRYKKSNKKLW* |
