Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030544.01.T01 | XP_013451021.1 | 96.629 | 89 | 3 | 0 | 1 | 89 | 369 | 457 | 1.29E-52 | 180 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030544.01.T01 | Q9ZWR8 | 47.297 | 74 | 39 | 0 | 16 | 89 | 395 | 468 | 1.14E-17 | 79 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030544.01.T01 | A0A072U6Q7 | 96.629 | 89 | 3 | 0 | 1 | 89 | 369 | 457 | 6.18e-53 | 180 |
| MsG0680030544.01.T01 | A0A396HA79 | 96.629 | 89 | 3 | 0 | 1 | 89 | 369 | 457 | 2.35e-51 | 182 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003731.01 | MsG0680030544.01 | 0.804604 | 1.994695e-49 | 1.032879e-46 |
| MsG0180005328.01 | MsG0680030544.01 | 0.818067 | 2.378630e-52 | 1.762281e-49 |
| MsG0580027797.01 | MsG0680030544.01 | 0.800671 | 1.292106e-48 | 6.052073e-46 |
| MsG0680030544.01 | MsG0680030545.01 | 0.882537 | 9.587360e-71 | 5.861129e-67 |
| MsG0680030544.01 | MsG0680030961.01 | 0.859790 | 3.241635e-63 | 8.665717e-60 |
| MsG0680030544.01 | MsG0780041079.01 | 0.800851 | 1.187155e-48 | 5.586149e-46 |
| MsG0680030544.01 | MsG0880045683.01 | 0.837739 | 4.350216e-57 | 5.704315e-54 |
| MsG0280006883.01 | MsG0680030544.01 | 0.800051 | 1.727547e-48 | 7.965294e-46 |
| MsG0280007052.01 | MsG0680030544.01 | 0.838010 | 3.706330e-57 | 4.900537e-54 |
| MsG0280008553.01 | MsG0680030544.01 | 0.823058 | 1.699219e-53 | 1.447561e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030544.01.T01 | MTR_6g015320 | 96.629 | 89 | 3 | 0 | 1 | 89 | 369 | 457 | 1.57e-56 | 180 |
| MsG0680030544.01.T01 | MTR_6g015315 | 78.652 | 89 | 16 | 1 | 1 | 89 | 370 | 455 | 3.73e-42 | 142 |
| MsG0680030544.01.T01 | MTR_6g015850 | 70.270 | 74 | 22 | 0 | 16 | 89 | 387 | 460 | 2.44e-31 | 114 |
| MsG0680030544.01.T01 | MTR_6g015815 | 70.270 | 74 | 22 | 0 | 16 | 89 | 390 | 463 | 2.53e-31 | 114 |
| MsG0680030544.01.T01 | MTR_6g015830 | 66.216 | 74 | 25 | 0 | 16 | 89 | 390 | 463 | 7.27e-30 | 110 |
| MsG0680030544.01.T01 | MTR_6g015310 | 71.875 | 64 | 15 | 1 | 1 | 64 | 273 | 333 | 6.24e-25 | 95.1 |
| MsG0680030544.01.T01 | MTR_7g014230 | 60.000 | 70 | 27 | 1 | 16 | 85 | 389 | 457 | 4.42e-22 | 88.6 |
| MsG0680030544.01.T01 | MTR_7g014330 | 57.534 | 73 | 30 | 1 | 16 | 88 | 387 | 458 | 1.96e-21 | 87.0 |
| MsG0680030544.01.T01 | MTR_7g014360 | 57.534 | 73 | 30 | 1 | 16 | 88 | 317 | 388 | 6.36e-21 | 85.1 |
| MsG0680030544.01.T01 | MTR_7g014610 | 48.052 | 77 | 40 | 0 | 12 | 88 | 401 | 477 | 3.99e-20 | 83.2 |
| MsG0680030544.01.T01 | MTR_7g014670 | 48.000 | 75 | 39 | 0 | 13 | 87 | 186 | 260 | 8.25e-20 | 80.5 |
| MsG0680030544.01.T01 | MTR_7g014200 | 42.857 | 84 | 48 | 0 | 3 | 86 | 385 | 468 | 2.06e-19 | 81.3 |
| MsG0680030544.01.T01 | MTR_7g014250 | 53.425 | 73 | 33 | 1 | 16 | 88 | 387 | 458 | 2.45e-19 | 80.9 |
| MsG0680030544.01.T01 | MTR_7g014160 | 42.857 | 84 | 48 | 0 | 3 | 86 | 390 | 473 | 2.62e-18 | 78.2 |
| MsG0680030544.01.T01 | MTR_2g089765 | 42.857 | 70 | 40 | 0 | 20 | 89 | 416 | 485 | 8.51e-16 | 70.9 |
| MsG0680030544.01.T01 | MTR_7g013850 | 41.176 | 85 | 50 | 0 | 2 | 86 | 396 | 480 | 1.91e-15 | 70.1 |
| MsG0680030544.01.T01 | MTR_2g089745 | 45.714 | 70 | 38 | 0 | 20 | 89 | 11 | 80 | 3.11e-15 | 64.7 |
| MsG0680030544.01.T01 | MTR_3g028210 | 42.857 | 70 | 40 | 0 | 20 | 89 | 398 | 467 | 4.18e-15 | 68.9 |
| MsG0680030544.01.T01 | MTR_7g014310 | 58.929 | 56 | 22 | 1 | 16 | 71 | 254 | 308 | 6.64e-15 | 68.2 |
| MsG0680030544.01.T01 | MTR_7g014620 | 46.875 | 64 | 34 | 0 | 23 | 86 | 369 | 432 | 4.22e-13 | 63.2 |
| MsG0680030544.01.T01 | MTR_2g089670 | 46.269 | 67 | 36 | 0 | 20 | 86 | 411 | 477 | 1.19e-12 | 62.0 |
| MsG0680030544.01.T01 | MTR_8g012750 | 41.333 | 75 | 42 | 1 | 17 | 89 | 401 | 475 | 1.74e-12 | 61.6 |
| MsG0680030544.01.T01 | MTR_3g435290 | 39.726 | 73 | 42 | 1 | 17 | 89 | 50 | 120 | 3.18e-12 | 58.2 |
| MsG0680030544.01.T01 | MTR_2g089755 | 36.364 | 77 | 47 | 1 | 13 | 89 | 404 | 478 | 1.05e-11 | 59.3 |
| MsG0680030544.01.T01 | MTR_2g089650 | 37.662 | 77 | 46 | 1 | 13 | 89 | 392 | 466 | 1.14e-11 | 59.3 |
| MsG0680030544.01.T01 | MTR_2g089735 | 40.000 | 70 | 40 | 1 | 20 | 89 | 397 | 464 | 1.77e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030544.01.T01 | AT3G29635 | 50.000 | 72 | 36 | 0 | 16 | 87 | 384 | 455 | 5.04e-18 | 77.4 |
| MsG0680030544.01.T01 | AT5G39050 | 43.836 | 73 | 41 | 0 | 16 | 88 | 397 | 469 | 1.70e-17 | 75.9 |
| MsG0680030544.01.T01 | AT3G29590 | 39.744 | 78 | 47 | 0 | 10 | 87 | 372 | 449 | 9.26e-16 | 70.9 |
| MsG0680030544.01.T01 | AT5G39090 | 41.667 | 72 | 42 | 0 | 16 | 87 | 377 | 448 | 1.42e-15 | 70.5 |
| MsG0680030544.01.T01 | AT3G29670 | 44.286 | 70 | 39 | 0 | 19 | 88 | 382 | 451 | 1.50e-15 | 70.5 |
| MsG0680030544.01.T01 | AT3G29680 | 43.478 | 69 | 39 | 0 | 19 | 87 | 380 | 448 | 3.25e-14 | 66.6 |
| MsG0680030544.01.T01 | AT1G03940 | 36.620 | 71 | 45 | 0 | 19 | 89 | 397 | 467 | 9.20e-14 | 65.1 |
| MsG0680030544.01.T01 | AT5G39080 | 37.313 | 67 | 42 | 0 | 16 | 82 | 391 | 457 | 3.29e-12 | 60.8 |
| MsG0680030544.01.T01 | AT3G29690 | 38.356 | 73 | 40 | 1 | 16 | 88 | 113 | 180 | 6.95e-12 | 58.5 |
Find 14 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTAACATCAACTTTCTT+AGG | 0.346906 | 6:+5061882 | None:intergenic |
| TACCGGGTCACCTAGGTTTG+AGG | 0.437629 | 6:-5061934 | MsG0680030544.01.T01:CDS |
| AATGATGGTGGAATTGAGAT+TGG | 0.442831 | 6:-5061816 | MsG0680030544.01.T01:CDS |
| GACTTTGAACAAGCAACAAA+TGG | 0.453802 | 6:-5061790 | MsG0680030544.01.T01:CDS |
| GCAATCTAAAACATCTAGAA+AGG | 0.487853 | 6:-5061982 | MsG0680030544.01.T01:CDS |
| GTAAATCCTTCAAACTTACA+AGG | 0.509431 | 6:+5061731 | None:intergenic |
| AAATTATACACCTCAAACCT+AGG | 0.524375 | 6:+5061924 | None:intergenic |
| TAAGAAAGTTGATGTTACTT+CGG | 0.530062 | 6:-5061880 | MsG0680030544.01.T01:CDS |
| CACCTCAAACCTAGGTGACC+CGG | 0.561487 | 6:+5061932 | None:intergenic |
| TCAGGAAATAGGAATAATGA+TGG | 0.563821 | 6:-5061831 | MsG0680030544.01.T01:CDS |
| GGAAATAGGAATAATGATGG+TGG | 0.570392 | 6:-5061828 | MsG0680030544.01.T01:CDS |
| TTTGAACAAGCAACAAATGG+AGG | 0.592025 | 6:-5061787 | MsG0680030544.01.T01:CDS |
| TTTCTATTACCGGGTCACCT+AGG | 0.609709 | 6:-5061941 | MsG0680030544.01.T01:CDS |
| GTTACTTCGGTTGATAGACC+AGG | 0.617818 | 6:-5061867 | MsG0680030544.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAAGATTTTTTCTATTAC+CGG | - | Chr6:5061788-5061807 | MsG0680030544.01.T01:CDS | 15.0% |
| !!! | AGGTGTATAATTTTGATTTT+GGG | - | Chr6:5061825-5061844 | MsG0680030544.01.T01:CDS | 20.0% |
| !!! | ATAAGATTTTTTCTATTACC+GGG | - | Chr6:5061789-5061808 | MsG0680030544.01.T01:CDS | 20.0% |
| !! | CTTTTTATCTTTCAGGAAAT+AGG | - | Chr6:5061897-5061916 | MsG0680030544.01.T01:CDS | 25.0% |
| !!! | GAGGTGTATAATTTTGATTT+TGG | - | Chr6:5061824-5061843 | MsG0680030544.01.T01:CDS | 25.0% |
| !!! | GTATAATTTTGATTTTGGGT+GGG | - | Chr6:5061829-5061848 | MsG0680030544.01.T01:CDS | 25.0% |
| !!! | TAAGAAAGTTGATGTTACTT+CGG | - | Chr6:5061859-5061878 | MsG0680030544.01.T01:CDS | 25.0% |
| !!! | TATAATTTTGATTTTGGGTG+GGG | - | Chr6:5061830-5061849 | MsG0680030544.01.T01:CDS | 25.0% |
| !!! | TGTATAATTTTGATTTTGGG+TGG | - | Chr6:5061828-5061847 | MsG0680030544.01.T01:CDS | 25.0% |
| AAATTATACACCTCAAACCT+AGG | + | Chr6:5061818-5061837 | None:intergenic | 30.0% | |
| GAAGTAACATCAACTTTCTT+AGG | + | Chr6:5061860-5061879 | None:intergenic | 30.0% | |
| TCAGGAAATAGGAATAATGA+TGG | - | Chr6:5061908-5061927 | MsG0680030544.01.T01:CDS | 30.0% | |
| ! | GCAATCTAAAACATCTAGAA+AGG | - | Chr6:5061757-5061776 | MsG0680030544.01.T01:CDS | 30.0% |
| GACTTTGAACAAGCAACAAA+TGG | - | Chr6:5061949-5061968 | MsG0680030544.01.T01:CDS | 35.0% | |
| GGAAATAGGAATAATGATGG+TGG | - | Chr6:5061911-5061930 | MsG0680030544.01.T01:CDS | 35.0% | |
| TTTGAACAAGCAACAAATGG+AGG | - | Chr6:5061952-5061971 | MsG0680030544.01.T01:CDS | 35.0% | |
| ! | TTTGCTCGACTTTTTGTTCA+AGG | - | Chr6:5061977-5061996 | MsG0680030544.01.T01:CDS | 35.0% |
| !! | AATGATGGTGGAATTGAGAT+TGG | - | Chr6:5061923-5061942 | MsG0680030544.01.T01:CDS | 35.0% |
| CCTGAAAGATAAAAAGCTCC+TGG | + | Chr6:5061893-5061912 | None:intergenic | 40.0% | |
| ! | CCAGGAGCTTTTTATCTTTC+AGG | - | Chr6:5061890-5061909 | MsG0680030544.01.T01:CDS | 40.0% |
| TTTCTATTACCGGGTCACCT+AGG | - | Chr6:5061798-5061817 | MsG0680030544.01.T01:CDS | 45.0% | |
| !! | GTTACTTCGGTTGATAGACC+AGG | - | Chr6:5061872-5061891 | MsG0680030544.01.T01:CDS | 45.0% |
| CACCTCAAACCTAGGTGACC+CGG | + | Chr6:5061810-5061829 | None:intergenic | 55.0% | |
| TACCGGGTCACCTAGGTTTG+AGG | - | Chr6:5061805-5061824 | MsG0680030544.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 5061746 | 5062015 | 5061746 | ID=MsG0680030544.01;Name=MsG0680030544.01 |
| Chr6 | mRNA | 5061746 | 5062015 | 5061746 | ID=MsG0680030544.01.T01;Parent=MsG0680030544.01;Name=MsG0680030544.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr6 | exon | 5061746 | 5062015 | 5061746 | ID=MsG0680030544.01.T01:exon:10966;Parent=MsG0680030544.01.T01 |
| Chr6 | CDS | 5061746 | 5062015 | 5061746 | ID=MsG0680030544.01.T01:cds;Parent=MsG0680030544.01.T01 |
| Gene Sequence |
| Protein sequence |
