AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003731.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003731.01.T01 MTR_1g069000 94.872 78 4 0 168 245 404 481 3.25e-46 160
MsG0180003731.01.T01 MTR_1g069000 73.913 92 15 1 10 92 42 133 1.21e-35 132
MsG0180003731.01.T01 MTR_1g069000 80.357 56 11 0 110 165 248 303 2.12e-20 89.7
MsG0180003731.01.T01 MTR_7g099830 58.947 95 30 1 10 95 42 136 1.34e-28 112
MsG0180003731.01.T01 MTR_7g099830 33.540 161 37 5 110 208 260 412 1.87e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003731.01.T01 AT2G37550 36.816 201 63 4 109 245 256 456 7.52e-27 107
MsG0180003731.01.T01 AT2G37550 57.778 90 29 1 10 90 42 131 2.21e-26 106
MsG0180003731.01.T01 AT2G37550 36.816 201 63 4 109 245 256 456 7.52e-27 107
MsG0180003731.01.T01 AT2G37550 57.778 90 29 1 10 90 42 131 2.21e-26 106
MsG0180003731.01.T01 AT3G53710 58.621 87 27 1 10 87 42 128 1.08e-24 102
MsG0180003731.01.T01 AT3G53710 47.059 85 35 2 168 245 378 459 1.81e-13 69.7
MsG0180003731.01.T01 AT3G53710 58.621 87 27 1 10 87 42 128 1.08e-24 102
MsG0180003731.01.T01 AT3G53710 47.059 85 35 2 168 245 378 459 1.81e-13 69.7
MsG0180003731.01.T01 AT3G53710 58.621 87 27 1 10 87 42 128 1.08e-24 102
MsG0180003731.01.T01 AT3G53710 47.059 85 35 2 168 245 378 459 1.81e-13 69.7

Find 89 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAAGCTGCTGGACATAA+TGG 0.311171 1:+67400737 MsG0180003731.01.T01:CDS
CACAAAAGAGATTACTTCTA+AGG 0.329121 1:+67399311 MsG0180003731.01.T01:CDS
ATCCCAGCTCTTGGGATGAT+TGG 0.345579 1:+67400585 MsG0180003731.01.T01:CDS
ATGATGCTGGTTGGGCTGGC+TGG 0.346920 1:+67400663 MsG0180003731.01.T01:CDS
CCGGTTGTGAAGGAGAATTT+AGG 0.374910 1:+67398661 MsG0180003731.01.T01:CDS
ATGATTCCGGCTGGGATGAT+TGG 0.384077 1:+67398724 MsG0180003731.01.T01:CDS
AGTTTATCGTGATCGGATTC+AGG 0.397292 1:+67398606 MsG0180003731.01.T01:CDS
CTTCCTTCCTAGAATCTTTG+TGG 0.398078 1:-67400610 None:intergenic
ATTGGGACCACAAAGATTCT+AGG 0.416709 1:+67400603 MsG0180003731.01.T01:CDS
CCACAATAATGATGCTGGTT+GGG 0.420450 1:+67400655 MsG0180003731.01.T01:CDS
TCCCAGCTCTTGGGATGATT+GGG 0.422693 1:+67400586 MsG0180003731.01.T01:CDS
CGCCGATGGGGCAGAGTCGC+CGG 0.424686 1:+67398697 MsG0180003731.01.T01:CDS
AATAATGATGCTGGTTGGGC+TGG 0.431540 1:+67400659 MsG0180003731.01.T01:CDS
TCGGTCGTGGCGTGATCCTC+CGG 0.431712 1:+67398642 MsG0180003731.01.T01:CDS
TTCCTTCGGAATAGAATACT+TGG 0.434318 1:-67398545 None:intergenic
TGATTCCGGCTGGGATGATT+GGG 0.435890 1:+67398725 MsG0180003731.01.T01:CDS
TACTCACAATCCCAGCTCTT+GGG 0.438114 1:+67400577 MsG0180003731.01.T01:CDS
TGAAGGAGAATTTAGGTTCA+AGG 0.439952 1:+67398668 MsG0180003731.01.T01:CDS
AAATCAGATGACGCTTGGAC+AGG 0.441545 1:+67400761 MsG0180003731.01.T01:CDS
GGGGATTGGTAAGTTGTCAT+TGG 0.442974 1:+67399254 MsG0180003731.01.T01:intron
AGAAAGCTGCTGGACATAAT+GGG 0.444118 1:+67400738 MsG0180003731.01.T01:CDS
CCTAAATTCTCCTTCACAAC+CGG 0.446662 1:-67398661 None:intergenic
TCCGGAAAACACCGTGGTCT+CGG 0.447372 1:+67398419 MsG0180003731.01.T01:CDS
CGGATTCAGGCTTTGTCGGA+AGG 0.463866 1:+67398619 MsG0180003731.01.T01:CDS
GTCCCAATCATCCCAAGAGC+TGG 0.464845 1:-67400588 None:intergenic
TGATCGGATTCAGGCTTTGT+CGG 0.466457 1:+67398615 MsG0180003731.01.T01:CDS
GTAACAATATCCGTTTCCTT+CGG 0.466590 1:-67398559 None:intergenic
TGGGGAGCCGATGCTTTAGC+TGG 0.469014 1:-67400635 None:intergenic
AATCAGATGACGCTTGGACA+GGG 0.470359 1:+67400762 MsG0180003731.01.T01:CDS
TGATGCTGGTTGGGCTGGCT+GGG 0.473809 1:+67400664 MsG0180003731.01.T01:CDS
GTACTCACAATCCCAGCTCT+TGG 0.474135 1:+67400576 MsG0180003731.01.T01:CDS
CTGCTTCAGTTTATCGTGAT+CGG 0.474442 1:+67398599 MsG0180003731.01.T01:CDS
GTTCAAGGGAAGGCCGCCGA+TGG 0.474588 1:+67398683 MsG0180003731.01.T01:CDS
CAGAGATTGGTCAAAGGACA+TGG 0.483037 1:+67400259 MsG0180003731.01.T01:CDS
CCCAACCAGCATCATTATTG+TGG 0.485486 1:-67400655 None:intergenic
AGCAGCGTTGGTGTTATACT+TGG 0.487190 1:-67398581 None:intergenic
TTCAAGGGAAGGCCGCCGAT+GGG 0.491943 1:+67398684 MsG0180003731.01.T01:CDS
ATTTAATTATCACAGGGGAT+TGG 0.494169 1:+67399240 MsG0180003731.01.T01:intron
AGTCGCCGGAGTAATGATTC+CGG 0.494324 1:+67398711 MsG0180003731.01.T01:CDS
CACTCAAAGACATCAGAGAT+TGG 0.494503 1:+67400246 MsG0180003731.01.T01:CDS
GCCGGAGTAATGATTCCGGC+TGG 0.503400 1:+67398715 MsG0180003731.01.T01:CDS
GCTGCTAATGCTGTTCAGAC+TGG 0.505364 1:+67399288 MsG0180003731.01.T01:CDS
GGAAGGCGGTTATGATCAAA+AGG 0.509923 1:+67400201 MsG0180003731.01.T01:CDS
GCATCTAATAAGAAAGCTGC+TGG 0.515421 1:+67400728 MsG0180003731.01.T01:CDS
CCCACAATAATGATGCTGGT+TGG 0.515926 1:+67400654 MsG0180003731.01.T01:CDS
CTTTGTCGGAAGGTCGGTCG+TGG 0.519720 1:+67398629 MsG0180003731.01.T01:CDS
CCAACCAGCATCATTATTGT+GGG 0.519854 1:-67400654 None:intergenic
ATGGGAAATCAGATGACGCT+TGG 0.522747 1:+67400756 MsG0180003731.01.T01:CDS
GATCCGTCACCATGGATGCT+TGG 0.527744 1:+67398462 MsG0180003731.01.T01:CDS
GATTTCGGACCAAGCATCCA+TGG 0.529426 1:-67398471 None:intergenic
CCGGCTGGGATGATTGGGAT+AGG 0.534746 1:+67398730 MsG0180003731.01.T01:CDS
ACGATAAACTGAAGCAGCGT+TGG 0.536105 1:-67398593 None:intergenic
GAAGGAGAATTTAGGTTCAA+GGG 0.536336 1:+67398669 MsG0180003731.01.T01:CDS
TTCAGGCTTTGTCGGAAGGT+CGG 0.544881 1:+67398623 MsG0180003731.01.T01:CDS
CTCCGGCGACTCTGCCCCAT+CGG 0.545912 1:-67398699 None:intergenic
GCTCCCCACAATAATGATGC+TGG 0.546441 1:+67400650 MsG0180003731.01.T01:CDS
GTATTCTATTCCGAAGGAAA+CGG 0.550054 1:+67398549 MsG0180003731.01.T01:CDS
GCGTGATCCTCCGGTTGTGA+AGG 0.563730 1:+67398651 MsG0180003731.01.T01:CDS
ATCAGATGACGCTTGGACAG+GGG 0.565298 1:+67400763 MsG0180003731.01.T01:CDS
TGCCAAGTATTCTATTCCGA+AGG 0.565535 1:+67398543 MsG0180003731.01.T01:CDS
TCTTTGTTTCAGGTCAAGGA+AGG 0.567944 1:+67400184 MsG0180003731.01.T01:intron
CGGCTGGGATGATTGGGATA+GGG 0.577559 1:+67398731 MsG0180003731.01.T01:CDS
TCGGAATAGAATACTTGGCA+AGG 0.577709 1:-67398540 None:intergenic
TCCCAATCATCCCAAGAGCT+GGG 0.579490 1:-67400587 None:intergenic
TTCATGTGCTTAGAATGCTC+CGG 0.579995 1:+67398401 None:intergenic
TGGGATAATGCAAAAGATGA+TGG 0.583815 1:+67400683 MsG0180003731.01.T01:CDS
CCTATCCCAATCATCCCAGC+CGG 0.584873 1:-67398730 None:intergenic
CCGGAGTAATGATTCCGGCT+GGG 0.585313 1:+67398716 MsG0180003731.01.T01:CDS
TCAAGGGAAGGCCGCCGATG+GGG 0.586047 1:+67398685 MsG0180003731.01.T01:CDS
TCAGATGACGCTTGGACAGG+GGG 0.587832 1:+67400764 MsG0180003731.01.T01:CDS
CCCAGCCGGAATCATTACTC+CGG 0.606336 1:-67398716 None:intergenic
AAGCATCCATGGTGACGGAT+CGG 0.610332 1:-67398460 None:intergenic
TTGTTTCAGGTCAAGGAAGG+CGG 0.612416 1:+67400187 MsG0180003731.01.T01:intron
AGAGATTGGTCAAAGGACAT+GGG 0.615964 1:+67400260 MsG0180003731.01.T01:CDS
GGACCAAGCATCCATGGTGA+CGG 0.618778 1:-67398465 None:intergenic
CATGGTGACGGATCGGACGA+AGG 0.619426 1:-67398453 None:intergenic
CGGCGACTCTGCCCCATCGG+CGG 0.622822 1:-67398696 None:intergenic
GAGAATTTAGGTTCAAGGGA+AGG 0.624220 1:+67398673 MsG0180003731.01.T01:CDS
CTTCGTCCGATCCGTCACCA+TGG 0.629444 1:+67398454 MsG0180003731.01.T01:CDS
GGAAGAACCAGCTAAAGCAT+CGG 0.631170 1:+67400628 MsG0180003731.01.T01:CDS
GATGATTGGGATAGGGAAGG+AGG 0.632481 1:+67398738 MsG0180003731.01.T01:CDS
GGACCACAAAGATTCTAGGA+AGG 0.638994 1:+67400607 MsG0180003731.01.T01:CDS
AGACATCAGAGATTGGTCAA+AGG 0.639485 1:+67400253 MsG0180003731.01.T01:CDS
GAGATTGGTCAAAGGACATG+GGG 0.646826 1:+67400261 MsG0180003731.01.T01:CDS
CAACCAGCATCATTATTGTG+GGG 0.653135 1:-67400653 None:intergenic
TGGGATGATTGGGATAGGGA+AGG 0.657232 1:+67398735 MsG0180003731.01.T01:CDS
AGATGTGAACACCGAGACCA+CGG 0.664997 1:-67398430 None:intergenic
AAATTCTCCTTCACAACCGG+AGG 0.674563 1:-67398658 None:intergenic
GAATGCTCCGGAAAACACCG+TGG 0.731228 1:+67398413 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTAGAAAGAAAAAA+TGG - Chr1:67400158-67400177 None:intergenic 10.0%
!! AAAAATTAGAAAGAAAAAAT+GGG - Chr1:67400157-67400176 None:intergenic 10.0%
!! TATTGTATTTAATTATCACA+GGG + Chr1:67399234-67399253 MsG0180003731.01.T01:intron 15.0%
!! TTATTGTATTTAATTATCAC+AGG + Chr1:67399233-67399252 MsG0180003731.01.T01:intron 15.0%
!! AGCTAAAAAGAAATAAATGA+AGG + Chr1:67399695-67399714 MsG0180003731.01.T01:intron 20.0%
!! ATGAAAAGAAAAGAACTAAT+TGG - Chr1:67400024-67400043 None:intergenic 20.0%
!! ATTGTATTTAATTATCACAG+GGG + Chr1:67399235-67399254 MsG0180003731.01.T01:intron 20.0%
!! GAAAATTTGTATTTGTTTCA+CGG + Chr1:67399389-67399408 MsG0180003731.01.T01:intron 20.0%
!! GCTAAAAAGAAATAAATGAA+GGG + Chr1:67399696-67399715 MsG0180003731.01.T01:intron 20.0%
!!! ATTTGGATTATTTTGTTCAT+GGG + Chr1:67399335-67399354 MsG0180003731.01.T01:intron 20.0%
!!! CTTTTCATTTTTGCATTAAT+TGG + Chr1:67400036-67400055 MsG0180003731.01.T01:intron 20.0%
!!! TATTTGGATTATTTTGTTCA+TGG + Chr1:67399334-67399353 MsG0180003731.01.T01:intron 20.0%
!!! TGATTTTATTTCAATTGCAT+TGG - Chr1:67399980-67399999 None:intergenic 20.0%
!!! TGTATTGATAATGTATTTGT+GGG + Chr1:67399751-67399770 MsG0180003731.01.T01:intron 20.0%
!!! TTCATTTATTTCTTTTTAGC+TGG - Chr1:67399696-67399715 None:intergenic 20.0%
! CAACAAATTGAACTAATACA+TGG - Chr1:67399438-67399457 None:intergenic 25.0%
! TGACTGCATTAAAAAGATAA+CGG - Chr1:67399657-67399676 None:intergenic 25.0%
! TGATTACATTCAATTACTAC+CGG + Chr1:67399133-67399152 MsG0180003731.01.T01:intron 25.0%
! TTGAATGTAATCAAATGTGT+CGG - Chr1:67399126-67399145 None:intergenic 25.0%
!! CTATTTGATAACACTTATCT+CGG - Chr1:67399548-67399567 None:intergenic 25.0%
!! GAAAAATAATCATTTTGCTG+TGG - Chr1:67399016-67399035 None:intergenic 25.0%
!!! CCATTTTTTGATTTGGATTT+CGG - Chr1:67398489-67398508 None:intergenic 25.0%
!!! GGATTTGATAACTTTTATGA+AGG + Chr1:67400704-67400723 MsG0180003731.01.T01:CDS 25.0%
!!! GTGTATTGATAATGTATTTG+TGG + Chr1:67399750-67399769 MsG0180003731.01.T01:intron 25.0%
!!! TTATTTTTCTGTTGTTTCAC+AGG + Chr1:67399027-67399046 MsG0180003731.01.T01:intron 25.0%
!!! TTTGGATTATTTTGTTCATG+GGG + Chr1:67399336-67399355 MsG0180003731.01.T01:intron 25.0%
!!! TTTTTTTTGCTCTTTGTTTC+AGG + Chr1:67400174-67400193 MsG0180003731.01.T01:intron 25.0%
ACTATCAGGATTTCAATCAA+GGG + Chr1:67400396-67400415 MsG0180003731.01.T01:intron 30.0%
ATTTAATTATCACAGGGGAT+TGG + Chr1:67399240-67399259 MsG0180003731.01.T01:intron 30.0%
CAAATTCGACTACTAATAAC+TGG + Chr1:67400345-67400364 MsG0180003731.01.T01:intron 30.0%
CACAAAAGAGATTACTTCTA+AGG + Chr1:67399311-67399330 MsG0180003731.01.T01:CDS 30.0%
CATGCTTTATAGTTGATTAG+TGG + Chr1:67399187-67399206 MsG0180003731.01.T01:intron 30.0%
CCGAAATCCAAATCAAAAAA+TGG + Chr1:67398486-67398505 MsG0180003731.01.T01:CDS 30.0%
CGAAATGAGAATGATAGAAA+TGG + Chr1:67400371-67400390 MsG0180003731.01.T01:intron 30.0%
TACTATCAGGATTTCAATCA+AGG + Chr1:67400395-67400414 MsG0180003731.01.T01:intron 30.0%
TGGAAGGAACAACTATAATA+AGG + Chr1:67400521-67400540 MsG0180003731.01.T01:intron 30.0%
! TATGACTTTTAGCATAGTGT+GGG + Chr1:67399911-67399930 MsG0180003731.01.T01:intron 30.0%
! TTATGACTTTTAGCATAGTG+TGG + Chr1:67399910-67399929 MsG0180003731.01.T01:intron 30.0%
!! GAGATTACTTCTAAGGTATT+TGG + Chr1:67399318-67399337 MsG0180003731.01.T01:intron 30.0%
!! GGGAATTGATAATGTTAACT+TGG + Chr1:67399771-67399790 MsG0180003731.01.T01:intron 30.0%
!!! ATAGCTGTTTTTCAATTCCA+CGG - Chr1:67399505-67399524 None:intergenic 30.0%
AAACTCAAAGCTCAAACTCT+TGG + Chr1:67400474-67400493 MsG0180003731.01.T01:intron 35.0%
AAAGGAGGTAATTCTTCTAC+TGG + Chr1:67400422-67400441 MsG0180003731.01.T01:intron 35.0%
AACTCAAAGCTCAAACTCTT+GGG + Chr1:67400475-67400494 MsG0180003731.01.T01:intron 35.0%
AATCAAAAAATGGAAGCTGG+TGG + Chr1:67398496-67398515 MsG0180003731.01.T01:CDS 35.0%
ACAGTGGATGTTAATTGGAT+CGG + Chr1:67399872-67399891 MsG0180003731.01.T01:intron 35.0%
ACATGGTAGAAATATCCCTA+AGG - Chr1:67399421-67399440 None:intergenic 35.0%
AGTCATATTCATATCTACGG+AGG - Chr1:67399103-67399122 None:intergenic 35.0%
CCAAATCAAAAAATGGAAGC+TGG + Chr1:67398493-67398512 MsG0180003731.01.T01:CDS 35.0%
CGGAGTCATATTCATATCTA+CGG - Chr1:67399106-67399125 None:intergenic 35.0%
GAAGAGAACAATCTTCATCA+GGG + Chr1:67400444-67400463 MsG0180003731.01.T01:intron 35.0%
GATTTCAATCAAGGGAACAA+AGG + Chr1:67400404-67400423 MsG0180003731.01.T01:intron 35.0%
GTAACAATATCCGTTTCCTT+CGG - Chr1:67398562-67398581 None:intergenic 35.0%
GTATTCTATTCCGAAGGAAA+CGG + Chr1:67398549-67398568 MsG0180003731.01.T01:CDS 35.0%
TCAGAACAGTGGATGTTAAT+TGG + Chr1:67399867-67399886 MsG0180003731.01.T01:intron 35.0%
TGATAGAAATGGCTACTATC+AGG + Chr1:67400382-67400401 MsG0180003731.01.T01:intron 35.0%
TGATGTGTGATTGACAATAC+TGG + Chr1:67399627-67399646 MsG0180003731.01.T01:intron 35.0%
TGGGATAATGCAAAAGATGA+TGG + Chr1:67400683-67400702 MsG0180003731.01.T01:CDS 35.0%
TTCCTTCGGAATAGAATACT+TGG - Chr1:67398548-67398567 None:intergenic 35.0%
! ATGACTTTTAGCATAGTGTG+GGG + Chr1:67399912-67399931 MsG0180003731.01.T01:intron 35.0%
! GAAGGAGAATTTAGGTTCAA+GGG + Chr1:67398669-67398688 MsG0180003731.01.T01:CDS 35.0%
! GTTCCTTCCAACTATCTTTT+TGG - Chr1:67400511-67400530 None:intergenic 35.0%
! TGAAGGAGAATTTAGGTTCA+AGG + Chr1:67398668-67398687 MsG0180003731.01.T01:CDS 35.0%
!! GATTTTAGGAATTTGAACGG+CGG + Chr1:67398794-67398813 MsG0180003731.01.T01:intron 35.0%
!! GCTTTTCATGGTATTTTTGC+TGG + Chr1:67400130-67400149 MsG0180003731.01.T01:intron 35.0%
!! GGGGATTTTAGGAATTTGAA+CGG + Chr1:67398791-67398810 MsG0180003731.01.T01:intron 35.0%
!! TGCTAACTGTTTGCTTTTCA+TGG + Chr1:67400118-67400137 MsG0180003731.01.T01:intron 35.0%
!!! ATTTTTTGCGAAGAAGATGG+CGG + Chr1:67398898-67398917 MsG0180003731.01.T01:intron 35.0%
!!! CCAGCTTCCATTTTTTGATT+TGG - Chr1:67398496-67398515 None:intergenic 35.0%
AAGAAAGCTGCTGGACATAA+TGG + Chr1:67400737-67400756 MsG0180003731.01.T01:CDS 40.0%
AAGGACATGGGGTTTAATGA+AGG + Chr1:67400272-67400291 MsG0180003731.01.T01:intron 40.0%
AGAAAGCTGCTGGACATAAT+GGG + Chr1:67400738-67400757 MsG0180003731.01.T01:CDS 40.0%
AGCTGTTGAATGAGGTCAAT+AGG + Chr1:67399943-67399962 MsG0180003731.01.T01:intron 40.0%
AGGACATGGGGTTTAATGAA+GGG + Chr1:67400273-67400292 MsG0180003731.01.T01:intron 40.0%
AGTACCTTCATGATTGTCTC+TGG + Chr1:67400077-67400096 MsG0180003731.01.T01:intron 40.0%
AGTTTATCGTGATCGGATTC+AGG + Chr1:67398606-67398625 MsG0180003731.01.T01:CDS 40.0%
ATTGGGACCACAAAGATTCT+AGG + Chr1:67400603-67400622 MsG0180003731.01.T01:CDS 40.0%
ATTGGGGCCAAAAAGATAGT+TGG + Chr1:67400501-67400520 MsG0180003731.01.T01:intron 40.0%
CAACCAGCATCATTATTGTG+GGG - Chr1:67400656-67400675 None:intergenic 40.0%
CAACTCCTTATTCATCTGGT+GGG + Chr1:67400546-67400565 MsG0180003731.01.T01:intron 40.0%
CACTCAAAGACATCAGAGAT+TGG + Chr1:67400246-67400265 MsG0180003731.01.T01:CDS 40.0%
CCAACCAGCATCATTATTGT+GGG - Chr1:67400657-67400676 None:intergenic 40.0%
CCTAAATTCTCCTTCACAAC+CGG - Chr1:67398664-67398683 None:intergenic 40.0%
CTAGTATGCTATAGCACTAC+AGG - Chr1:67399578-67399597 None:intergenic 40.0%
CTGCTTCAGTTTATCGTGAT+CGG + Chr1:67398599-67398618 MsG0180003731.01.T01:CDS 40.0%
CTTCCTTCCTAGAATCTTTG+TGG - Chr1:67400613-67400632 None:intergenic 40.0%
GCATCTAATAAGAAAGCTGC+TGG + Chr1:67400728-67400747 MsG0180003731.01.T01:CDS 40.0%
GCTATCTGATAACACTGTAG+TGG - Chr1:67399474-67399493 None:intergenic 40.0%
GCTGTTGAATGAGGTCAATA+GGG + Chr1:67399944-67399963 MsG0180003731.01.T01:intron 40.0%
GGAAGAGAACAATCTTCATC+AGG + Chr1:67400443-67400462 MsG0180003731.01.T01:intron 40.0%
GGATCAACTCCTTATTCATC+TGG + Chr1:67400542-67400561 MsG0180003731.01.T01:intron 40.0%
TAGAGGAAAGCTGTTGAATG+AGG + Chr1:67399935-67399954 MsG0180003731.01.T01:intron 40.0%
TATTTATACGCGGTCGCAAT+TGG + Chr1:67398850-67398869 MsG0180003731.01.T01:intron 40.0%
TCAACTCCTTATTCATCTGG+TGG + Chr1:67400545-67400564 MsG0180003731.01.T01:intron 40.0%
TCATGACTCGTCGAAAACAA+AGG - Chr1:67399072-67399091 None:intergenic 40.0%
TCGGAATAGAATACTTGGCA+AGG - Chr1:67398543-67398562 None:intergenic 40.0%
TGCCAAGTATTCTATTCCGA+AGG + Chr1:67398543-67398562 MsG0180003731.01.T01:CDS 40.0%
TTCAATCAAGGGAACAAAGG+AGG + Chr1:67400407-67400426 MsG0180003731.01.T01:intron 40.0%
! AGACATCAGAGATTGGTCAA+AGG + Chr1:67400253-67400272 MsG0180003731.01.T01:CDS 40.0%
! AGAGATTGGTCAAAGGACAT+GGG + Chr1:67400260-67400279 MsG0180003731.01.T01:CDS 40.0%
! CCACAATAATGATGCTGGTT+GGG + Chr1:67400655-67400674 MsG0180003731.01.T01:CDS 40.0%
! GAGAATTTAGGTTCAAGGGA+AGG + Chr1:67398673-67398692 MsG0180003731.01.T01:CDS 40.0%
! TCTTTGTTTCAGGTCAAGGA+AGG + Chr1:67400184-67400203 MsG0180003731.01.T01:intron 40.0%
! TTGCTCTTTGTTTCAGGTCA+AGG + Chr1:67400180-67400199 MsG0180003731.01.T01:intron 40.0%
!!! GGGATTTTTTGCGAAGAAGA+TGG + Chr1:67398895-67398914 MsG0180003731.01.T01:intron 40.0%
AAATCAGATGACGCTTGGAC+AGG + Chr1:67400761-67400780 MsG0180003731.01.T01:CDS 45.0%
AAATTCTCCTTCACAACCGG+AGG - Chr1:67398661-67398680 None:intergenic 45.0%
AATCAGATGACGCTTGGACA+GGG + Chr1:67400762-67400781 MsG0180003731.01.T01:CDS 45.0%
ACGATAAACTGAAGCAGCGT+TGG - Chr1:67398596-67398615 None:intergenic 45.0%
ATGGGAAATCAGATGACGCT+TGG + Chr1:67400756-67400775 MsG0180003731.01.T01:CDS 45.0%
CCCAACCAGCATCATTATTG+TGG - Chr1:67400658-67400677 None:intergenic 45.0%
CCCACAATAATGATGCTGGT+TGG + Chr1:67400654-67400673 MsG0180003731.01.T01:CDS 45.0%
CCGGTTGTGAAGGAGAATTT+AGG + Chr1:67398661-67398680 MsG0180003731.01.T01:CDS 45.0%
CGACGGAGGATATTTATACG+CGG + Chr1:67398840-67398859 MsG0180003731.01.T01:intron 45.0%
CTCAAACTCTTGGGACGATT+GGG + Chr1:67400484-67400503 MsG0180003731.01.T01:intron 45.0%
GACATGGGGTTTAATGAAGG+GGG + Chr1:67400275-67400294 MsG0180003731.01.T01:intron 45.0%
GGAAGAACCAGCTAAAGCAT+CGG + Chr1:67400628-67400647 MsG0180003731.01.T01:CDS 45.0%
GGAAGGCGGTTATGATCAAA+AGG + Chr1:67400201-67400220 MsG0180003731.01.T01:CDS 45.0%
GGACATGGGGTTTAATGAAG+GGG + Chr1:67400274-67400293 MsG0180003731.01.T01:intron 45.0%
GGGCCAAAAAGATAGTTGGA+AGG + Chr1:67400505-67400524 MsG0180003731.01.T01:intron 45.0%
GTTTCACGGAATGCACCTTA+GGG + Chr1:67399403-67399422 MsG0180003731.01.T01:intron 45.0%
TACTCACAATCCCAGCTCTT+GGG + Chr1:67400577-67400596 MsG0180003731.01.T01:CDS 45.0%
TAGCAGACGTATCTTGATGC+CGG - Chr1:67400103-67400122 None:intergenic 45.0%
TCAAACTCTTGGGACGATTG+GGG + Chr1:67400485-67400504 MsG0180003731.01.T01:intron 45.0%
TCAGATAGCTGATATAGCCG+TGG + Chr1:67399485-67399504 MsG0180003731.01.T01:intron 45.0%
TCTCGGCATATCACAAATCG+TGG - Chr1:67399531-67399550 None:intergenic 45.0%
TGATGTGAGGAGTAAGTCGA+CGG + Chr1:67398770-67398789 MsG0180003731.01.T01:intron 45.0%
TGTTTCACGGAATGCACCTT+AGG + Chr1:67399402-67399421 MsG0180003731.01.T01:intron 45.0%
TTTAGCATAGTGTGGGGTAG+AGG + Chr1:67399918-67399937 MsG0180003731.01.T01:intron 45.0%
TTTAGGAATTTGAACGGCGG+TGG + Chr1:67398797-67398816 MsG0180003731.01.T01:intron 45.0%
! AATAATGATGCTGGTTGGGC+TGG + Chr1:67400659-67400678 MsG0180003731.01.T01:CDS 45.0%
! AGTAAGTCGACGGGGATTTT+AGG + Chr1:67398780-67398799 MsG0180003731.01.T01:intron 45.0%
! CAGAGATTGGTCAAAGGACA+TGG + Chr1:67400259-67400278 MsG0180003731.01.T01:CDS 45.0%
! GAGATTGGTCAAAGGACATG+GGG + Chr1:67400261-67400280 MsG0180003731.01.T01:CDS 45.0%
! GGACCACAAAGATTCTAGGA+AGG + Chr1:67400607-67400626 MsG0180003731.01.T01:CDS 45.0%
! GGGGATTGGTAAGTTGTCAT+TGG + Chr1:67399254-67399273 MsG0180003731.01.T01:intron 45.0%
! TGATCGGATTCAGGCTTTGT+CGG + Chr1:67398615-67398634 MsG0180003731.01.T01:CDS 45.0%
! TGTGGATTGCTCCTTTGTGA+AGG - Chr1:67398998-67399017 None:intergenic 45.0%
! TTGCTCCTTTGTGAAGGCAT+CGG - Chr1:67398992-67399011 None:intergenic 45.0%
! TTGTTTCAGGTCAAGGAAGG+CGG + Chr1:67400187-67400206 MsG0180003731.01.T01:intron 45.0%
!! AGCAGCGTTGGTGTTATACT+TGG - Chr1:67398584-67398603 None:intergenic 45.0%
AATGAAGGGGGTCATGGCTT+TGG + Chr1:67400287-67400306 MsG0180003731.01.T01:intron 50.0%
ACATACTTCCCACCTTGCGA+CGG - Chr1:67398953-67398972 None:intergenic 50.0%
AGATGTGAACACCGAGACCA+CGG - Chr1:67398433-67398452 None:intergenic 50.0%
AGTCGCCGGAGTAATGATTC+CGG + Chr1:67398711-67398730 MsG0180003731.01.T01:CDS 50.0%
ATCAGATGACGCTTGGACAG+GGG + Chr1:67400763-67400782 MsG0180003731.01.T01:CDS 50.0%
ATCCCAGCTCTTGGGATGAT+TGG + Chr1:67400585-67400604 MsG0180003731.01.T01:CDS 50.0%
ATGATTCCGGCTGGGATGAT+TGG + Chr1:67398724-67398743 MsG0180003731.01.T01:CDS 50.0%
ATGTGAGGAGTAAGTCGACG+GGG + Chr1:67398772-67398791 MsG0180003731.01.T01:intron 50.0%
CCTTCATGATTGTCTCTGGC+CGG + Chr1:67400081-67400100 MsG0180003731.01.T01:intron 50.0%
CTAGTCCGATGCCTTCACAA+AGG + Chr1:67398984-67399003 MsG0180003731.01.T01:intron 50.0%
GACTGCCCACCAGATGAATA+AGG - Chr1:67400554-67400573 None:intergenic 50.0%
GATGATTGGGATAGGGAAGG+AGG + Chr1:67398738-67398757 MsG0180003731.01.T01:CDS 50.0%
GATGTGAGGAGTAAGTCGAC+GGG + Chr1:67398771-67398790 MsG0180003731.01.T01:intron 50.0%
GATTTCGGACCAAGCATCCA+TGG - Chr1:67398474-67398493 None:intergenic 50.0%
GCTCAAACTCTTGGGACGAT+TGG + Chr1:67400483-67400502 MsG0180003731.01.T01:intron 50.0%
GCTCCCCACAATAATGATGC+TGG + Chr1:67400650-67400669 MsG0180003731.01.T01:CDS 50.0%
GCTGCTAATGCTGTTCAGAC+TGG + Chr1:67399288-67399307 MsG0180003731.01.T01:CDS 50.0%
GGGTTTAATGAAGGGGGTCA+TGG + Chr1:67400281-67400300 MsG0180003731.01.T01:intron 50.0%
GTACTCACAATCCCAGCTCT+TGG + Chr1:67400576-67400595 MsG0180003731.01.T01:CDS 50.0%
GTCGCAATTGGAAGCTTCTG+CGG + Chr1:67398862-67398881 MsG0180003731.01.T01:intron 50.0%
TCCCAATCATCCCAAGAGCT+GGG - Chr1:67400590-67400609 None:intergenic 50.0%
TCCCAGCTCTTGGGATGATT+GGG + Chr1:67400586-67400605 MsG0180003731.01.T01:CDS 50.0%
TGATTCCGGCTGGGATGATT+GGG + Chr1:67398725-67398744 MsG0180003731.01.T01:CDS 50.0%
TGGGATGATTGGGATAGGGA+AGG + Chr1:67398735-67398754 MsG0180003731.01.T01:CDS 50.0%
TTGGGATAGGGAAGGAGGTT+CGG + Chr1:67398743-67398762 MsG0180003731.01.T01:intron 50.0%
! ATCCACTGTTCTGAGCAACG+AGG - Chr1:67399861-67399880 None:intergenic 50.0%
! CGCAAGGTGGGAAGTATGTT+GGG + Chr1:67398954-67398973 MsG0180003731.01.T01:intron 50.0%
! CTTTGGCTTCACGAAGGTTG+AGG + Chr1:67400304-67400323 MsG0180003731.01.T01:intron 50.0%
! GGTGGGAAGTATGTTGGGTT+CGG + Chr1:67398959-67398978 MsG0180003731.01.T01:intron 50.0%
! TATTCATCTGGTGGGCAGTC+TGG + Chr1:67400554-67400573 MsG0180003731.01.T01:intron 50.0%
! TCGCAAGGTGGGAAGTATGT+TGG + Chr1:67398953-67398972 MsG0180003731.01.T01:intron 50.0%
! TTCAGGCTTTGTCGGAAGGT+CGG + Chr1:67398623-67398642 MsG0180003731.01.T01:CDS 50.0%
!! AAGCATCCATGGTGACGGAT+CGG - Chr1:67398463-67398482 None:intergenic 50.0%
!! AGATGGCGGAGAATGAGTCA+AGG + Chr1:67398912-67398931 MsG0180003731.01.T01:intron 50.0%
!! TCATGGCTTTGGCTTCACGA+AGG + Chr1:67400298-67400317 MsG0180003731.01.T01:intron 50.0%
AGCTTCTGCGGCGAGTAAAG+AGG + Chr1:67398874-67398893 MsG0180003731.01.T01:intron 55.0%
CCCAGCCGGAATCATTACTC+CGG - Chr1:67398719-67398738 None:intergenic 55.0%
CCGGAGTAATGATTCCGGCT+GGG + Chr1:67398716-67398735 MsG0180003731.01.T01:CDS 55.0%
CCGGCCAGAGACAATCATGA+AGG - Chr1:67400084-67400103 None:intergenic 55.0%
CCTATCCCAATCATCCCAGC+CGG - Chr1:67398733-67398752 None:intergenic 55.0%
CGGCTGGGATGATTGGGATA+GGG + Chr1:67398731-67398750 MsG0180003731.01.T01:CDS 55.0%
GAGAATGAGTCAAGGCCGGA+AGG + Chr1:67398920-67398939 MsG0180003731.01.T01:intron 55.0%
GATCCGTCACCATGGATGCT+TGG + Chr1:67398462-67398481 MsG0180003731.01.T01:CDS 55.0%
GCATGCACACACACACACAC+CGG - Chr1:67399172-67399191 None:intergenic 55.0%
GCTTCTGCGGCGAGTAAAGA+GGG + Chr1:67398875-67398894 MsG0180003731.01.T01:intron 55.0%
GGACCAAGCATCCATGGTGA+CGG - Chr1:67398468-67398487 None:intergenic 55.0%
GTCCCAATCATCCCAAGAGC+TGG - Chr1:67400591-67400610 None:intergenic 55.0%
TCAGATGACGCTTGGACAGG+GGG + Chr1:67400764-67400783 MsG0180003731.01.T01:CDS 55.0%
TCCGGAAAACACCGTGGTCT+CGG + Chr1:67398419-67398438 MsG0180003731.01.T01:CDS 55.0%
TGCCTCGTTGCTCAGAACAG+TGG + Chr1:67399856-67399875 MsG0180003731.01.T01:intron 55.0%
TGGGATAGGGAAGGAGGTTC+GGG + Chr1:67398744-67398763 MsG0180003731.01.T01:intron 55.0%
! ACCGAGACCACGGTGTTTTC+CGG - Chr1:67398423-67398442 None:intergenic 55.0%
! CGGATTCAGGCTTTGTCGGA+AGG + Chr1:67398619-67398638 MsG0180003731.01.T01:CDS 55.0%
! TACCGGTGTCAGTGTTGTGC+CGG + Chr1:67399150-67399169 MsG0180003731.01.T01:intron 55.0%
CACCGGCACAACACTGACAC+CGG - Chr1:67399155-67399174 None:intergenic 60.0%
CCGGCTGGGATGATTGGGAT+AGG + Chr1:67398730-67398749 MsG0180003731.01.T01:CDS 60.0%
CTTCGTCCGATCCGTCACCA+TGG + Chr1:67398454-67398473 MsG0180003731.01.T01:CDS 60.0%
CTTTGTCGGAAGGTCGGTCG+TGG + Chr1:67398629-67398648 MsG0180003731.01.T01:CDS 60.0%
GAAGGACTTCCACCGTCGCA+AGG + Chr1:67398938-67398957 MsG0180003731.01.T01:intron 60.0%
GACTTCCACCGTCGCAAGGT+GGG + Chr1:67398942-67398961 MsG0180003731.01.T01:intron 60.0%
GCCGGAGTAATGATTCCGGC+TGG + Chr1:67398715-67398734 MsG0180003731.01.T01:CDS 60.0%
GCGTGATCCTCCGGTTGTGA+AGG + Chr1:67398651-67398670 MsG0180003731.01.T01:CDS 60.0%
GGCGGAGAATGAGTCAAGGC+CGG + Chr1:67398916-67398935 MsG0180003731.01.T01:intron 60.0%
TACTTCCCACCTTGCGACGG+TGG - Chr1:67398950-67398969 None:intergenic 60.0%
TGCGACGGTGGAAGTCCTTC+CGG - Chr1:67398938-67398957 None:intergenic 60.0%
TGGGGAGCCGATGCTTTAGC+TGG - Chr1:67400638-67400657 None:intergenic 60.0%
TTCAAGGGAAGGCCGCCGAT+GGG + Chr1:67398684-67398703 MsG0180003731.01.T01:CDS 60.0%
TTTGAACGGCGGTGGTGCAC+CGG + Chr1:67398805-67398824 MsG0180003731.01.T01:intron 60.0%
!! ATGATGCTGGTTGGGCTGGC+TGG + Chr1:67400663-67400682 MsG0180003731.01.T01:CDS 60.0%
!! CATGGTGACGGATCGGACGA+AGG - Chr1:67398456-67398475 None:intergenic 60.0%
!! GAGGTTCGGGTGCTGATGTG+AGG + Chr1:67398757-67398776 MsG0180003731.01.T01:intron 60.0%
!! TGATGCTGGTTGGGCTGGCT+GGG + Chr1:67400664-67400683 MsG0180003731.01.T01:CDS 60.0%
ACCGGCGAGATCGAGGTCGA+CGG + Chr1:67398823-67398842 MsG0180003731.01.T01:intron 65.0%
GATGACGCTTGGACAGGGGG+TGG + Chr1:67400767-67400786 MsG0180003731.01.T01:CDS 65.0%
GGACTTCCACCGTCGCAAGG+TGG + Chr1:67398941-67398960 MsG0180003731.01.T01:intron 65.0%
GTTCAAGGGAAGGCCGCCGA+TGG + Chr1:67398683-67398702 MsG0180003731.01.T01:CDS 65.0%
TCAAGGGAAGGCCGCCGATG+GGG + Chr1:67398685-67398704 MsG0180003731.01.T01:CDS 65.0%
TCCGTCGACCTCGATCTCGC+CGG - Chr1:67398827-67398846 None:intergenic 65.0%
TCGGTCGTGGCGTGATCCTC+CGG + Chr1:67398642-67398661 MsG0180003731.01.T01:CDS 65.0%
CTCCGGCGACTCTGCCCCAT+CGG - Chr1:67398702-67398721 None:intergenic 70.0%
GGCGAGATCGAGGTCGACGG+AGG + Chr1:67398826-67398845 MsG0180003731.01.T01:intron 70.0%
! GTGGTGCACCGGCGAGATCG+AGG + Chr1:67398816-67398835 MsG0180003731.01.T01:intron 70.0%
CGCCGATGGGGCAGAGTCGC+CGG + Chr1:67398697-67398716 MsG0180003731.01.T01:CDS 75.0%
CGGCGACTCTGCCCCATCGG+CGG - Chr1:67398699-67398718 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 67398415 67400799 67398415 ID=MsG0180003731.01;Name=MsG0180003731.01
Chr1 mRNA 67398415 67400799 67398415 ID=MsG0180003731.01.T01;Parent=MsG0180003731.01;Name=MsG0180003731.01.T01;_AED=0.40;_eAED=0.47;_QI=0|0.33|0.25|1|0.66|0.75|4|0|245
Chr1 exon 67398415 67398759 67398415 ID=MsG0180003731.01.T01:exon:24047;Parent=MsG0180003731.01.T01
Chr1 exon 67399255 67399332 67399255 ID=MsG0180003731.01.T01:exon:24048;Parent=MsG0180003731.01.T01
Chr1 exon 67400196 67400282 67400196 ID=MsG0180003731.01.T01:exon:24049;Parent=MsG0180003731.01.T01
Chr1 exon 67400572 67400799 67400572 ID=MsG0180003731.01.T01:exon:24050;Parent=MsG0180003731.01.T01
Chr1 CDS 67398415 67398759 67398415 ID=MsG0180003731.01.T01:cds;Parent=MsG0180003731.01.T01
Chr1 CDS 67399255 67399332 67399255 ID=MsG0180003731.01.T01:cds;Parent=MsG0180003731.01.T01
Chr1 CDS 67400196 67400282 67400196 ID=MsG0180003731.01.T01:cds;Parent=MsG0180003731.01.T01
Chr1 CDS 67400572 67400799 67400572 ID=MsG0180003731.01.T01:cds;Parent=MsG0180003731.01.T01
Gene Sequence

>MsG0180003731.01.T01

ATGCTCCGGAAAACACCGTGGTCTCGGTGTTCACATCTCCTTCGTCCGATCCGTCACCATGGATGCTTGGTCCGAAATCCAAATCAAAAAATGGAAGCTGGTGGCAATGACAATCTCAACGCTTTCCTTGCCAAGTATTCTATTCCGAAGGAAACGGATATTGTTACCAAGTATAACACCAACGCTGCTTCAGTTTATCGTGATCGGATTCAGGCTTTGTCGGAAGGTCGGTCGTGGCGTGATCCTCCGGTTGTGAAGGAGAATTTAGGTTCAAGGGAAGGCCGCCGATGGGGCAGAGTCGCCGGAGTAATGATTCCGGCTGGGATGATTGGGATAGGGAAGGAGGGGATTGGTAAGTTGTCATTGGTTGCTGCATCTGCTGCTAATGCTGTTCAGACTGGCACAAAAGAGATTACTTCTAAGGTCAAGGAAGGCGGTTATGATCAAAAGGTCAACGAAACTGTCACTGTTGTCACTCAAAGACATCAGAGATTGGTCAAAGGACATGGGTCTGGTACTCACAATCCCAGCTCTTGGGATGATTGGGACCACAAAGATTCTAGGAAGGAAGAACCAGCTAAAGCATCGGCTCCCCACAATAATGATGCTGGTTGGGCTGGCTGGGATAATGCAAAAGATGATGGATTTGATAACTTTTATGAAGGTGCATCTAATAAGAAAGCTGCTGGACATAATGGGAAATCAGATGACGCTTGGACAGGGGGTGGCTTCCTCTAA
Protein sequence

>MsG0180003731.01.T01

MLRKTPWSRCSHLLRPIRHHGCLVRNPNQKMEAGGNDNLNAFLAKYSIPKETDIVTKYNTNAASVYRDRIQALSEGRSWRDPPVVKENLGSREGRRWGRVAGVMIPAGMIGIGKEGIGKLSLVAASAANAVQTGTKEITSKVKEGGYDQKVNETVTVVTQRHQRLVKGHGSGTHNPSSWDDWDHKDSRKEEPAKASAPHNNDAGWAGWDNAKDDGFDNFYEGASNKKAAGHNGKSDDAWTGGGFL*