AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030561.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030561.01.T01 MTR_3g091650 88.281 128 15 0 66 193 203 330 1.40e-71 229
MsG0680030561.01.T01 MTR_3g091650 88.281 128 15 0 66 193 203 330 8.97e-71 229
MsG0680030561.01.T01 MTR_8g012340 60.000 105 37 3 9 113 294 393 1.18e-28 112
MsG0680030561.01.T01 MTR_8g011560 54.630 108 41 3 9 113 26 128 2.76e-25 98.6
MsG0680030561.01.T01 MTR_8g010620 54.717 106 42 3 9 113 96 196 3.46e-24 97.1
MsG0680030561.01.T01 MTR_8g011100 55.660 106 41 3 9 113 210 310 8.49e-24 97.4
MsG0680030561.01.T01 MTR_8g010620 54.717 106 42 3 9 113 163 263 9.04e-24 97.1
MsG0680030561.01.T01 MTR_8g010620 54.717 106 42 3 9 113 224 324 1.18e-23 97.4
MsG0680030561.01.T01 MTR_8g010620 54.717 106 42 3 9 113 199 299 1.20e-23 97.1
MsG0680030561.01.T01 MTR_7g053470 51.852 108 45 4 9 116 288 388 1.11e-21 92.0
MsG0680030561.01.T01 MTR_8g038785 67.606 71 13 2 2 68 418 482 3.23e-20 88.2
MsG0680030561.01.T01 MTR_8g008640 50.000 100 48 2 12 111 222 319 6.23e-19 84.3
MsG0680030561.01.T01 MTR_8g080980 48.148 108 49 3 9 116 292 392 1.37e-18 83.2
MsG0680030561.01.T01 MTR_8g011140 69.091 55 14 1 9 60 167 221 5.42e-17 77.0
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 78 135 3.27e-12 64.3
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 162 219 3.83e-12 64.3
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 78 135 4.02e-12 64.3
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 162 219 4.43e-12 64.3
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 264 321 4.75e-12 64.3
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 288 345 5.83e-12 63.9
MsG0680030561.01.T01 MTR_1g017870 55.000 60 25 1 7 66 288 345 6.51e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030561.01.T01 AT3G25230 58.824 85 33 2 9 93 293 375 8.32e-21 89.7
MsG0680030561.01.T01 AT3G25230 58.824 85 33 2 9 93 293 375 1.00e-20 89.4
MsG0680030561.01.T01 AT5G48570 48.624 109 49 4 9 116 302 404 7.43e-18 81.3
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.84e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.84e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 161 269 6.91e-16 75.5
MsG0680030561.01.T01 AT5G53020 39.844 128 56 4 66 191 152 260 7.58e-16 75.5

Find 26 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATTTCACAAGGATT+TGG 0.229217 6:-5294024 MsG0680030561.01.T01:CDS
ATCTTTGCAGTGAAAGTAAT+TGG 0.273802 6:-5294761 MsG0680030561.01.T01:intron
TTAAGTTATCAAAGGTTAGA+AGG 0.298370 6:-5293920 MsG0680030561.01.T01:CDS
CGGTCGCACTGTTATGCATT+TGG 0.321613 6:-5294129 MsG0680030561.01.T01:CDS
GCTAAGATAAGCAAAATTTC+AGG 0.349111 6:-5295048 MsG0680030561.01.T01:intron
CAAAGAGGAGTCTGATTTGA+TGG 0.372221 6:-5294069 MsG0680030561.01.T01:CDS
TGCTACCTCACCCTTCTTCA+TGG 0.373091 6:+5294504 None:intergenic
TGAGCAGAGCAAGTGATTGA+TGG 0.470924 6:-5294547 MsG0680030561.01.T01:intron
AGACATCTTGCGACAGAACT+CGG 0.491610 6:-5294149 MsG0680030561.01.T01:CDS
GATGGTTCAGAAATTCACTT+TGG 0.497055 6:-5294051 MsG0680030561.01.T01:CDS
GAAAGAGGTTAAGTTATCAA+AGG 0.511514 6:-5293928 MsG0680030561.01.T01:CDS
GGAGGAACTTATTTCTGTCA+TGG 0.518018 6:-5294096 MsG0680030561.01.T01:CDS
AGCTGTAATGACCATGAAGA+AGG 0.522299 6:-5294515 MsG0680030561.01.T01:CDS
AAAGCAAATGCTGTTGAAAG+AGG 0.523700 6:-5293943 MsG0680030561.01.T01:CDS
GAGCAGAGCAAGTGATTGAT+GGG 0.549453 6:-5294546 MsG0680030561.01.T01:intron
TATGCATTTGGATGCTACAA+AGG 0.577556 6:-5294117 MsG0680030561.01.T01:CDS
GTTTGAATTCACAAGAGATG+AGG 0.584171 6:-5294678 MsG0680030561.01.T01:CDS
TTTGAATTCACAAGAGATGA+GGG 0.588141 6:-5294677 MsG0680030561.01.T01:intron
TTCTGTCATGGAGAACAAAG+AGG 0.590110 6:-5294084 MsG0680030561.01.T01:CDS
AAATGGAGAGAAGCTTCAGA+AGG 0.605319 6:-5293873 MsG0680030561.01.T01:CDS
GCTGTAATGACCATGAAGAA+GGG 0.607177 6:-5294514 MsG0680030561.01.T01:CDS
AATGACCATGAAGAAGGGTG+AGG 0.610564 6:-5294509 MsG0680030561.01.T01:CDS
GCATTTGGATGCTACAAAGG+AGG 0.627843 6:-5294114 MsG0680030561.01.T01:CDS
GGAGATTGCAAAATTTCACA+AGG 0.637872 6:-5294030 MsG0680030561.01.T01:CDS
GCGAACATAAGCGAAATGCA+AGG 0.645192 6:-5295072 None:intergenic
GCTCTGTTTCATCATCGCCA+CGG 0.673900 6:+5294702 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCATTGAAAACTAAATG+AGG + Chr6:5294444-5294463 None:intergenic 20.0%
!! TATATTAAAATCATGTCCTA+TGG - Chr6:5294642-5294661 MsG0680030561.01.T01:intron 20.0%
!! TGGTACAAAAACAAATTTAT+GGG + Chr6:5294262-5294281 None:intergenic 20.0%
!! TTGGTACAAAAACAAATTTA+TGG + Chr6:5294263-5294282 None:intergenic 20.0%
!!! GTTTTTTTACTATGATACTT+TGG - Chr6:5294563-5294582 MsG0680030561.01.T01:intron 20.0%
! CGTTAGTAATTTCAATCTTT+AGG + Chr6:5294544-5294563 None:intergenic 25.0%
! TATCAAAATATTGTCAAGGA+AGG - Chr6:5293965-5293984 MsG0680030561.01.T01:CDS 25.0%
! TGTAAAGCTTTCAAAATACA+AGG - Chr6:5293866-5293885 MsG0680030561.01.T01:CDS 25.0%
! TTAAGTTATCAAAGGTTAGA+AGG - Chr6:5294958-5294977 MsG0680030561.01.T01:intron 25.0%
AATAACATTCTTCTGATCTC+AGG - Chr6:5294667-5294686 MsG0680030561.01.T01:intron 30.0%
ACAAAGCAACAAAATCAAAG+AGG + Chr6:5293892-5293911 None:intergenic 30.0%
AGAAGAATGTTATTGACCAT+AGG + Chr6:5294661-5294680 None:intergenic 30.0%
ATCATCATTGATGCTAATGA+AGG - Chr6:5294033-5294052 MsG0680030561.01.T01:CDS 30.0%
ATCTTTGCAGTGAAAGTAAT+TGG - Chr6:5294117-5294136 MsG0680030561.01.T01:CDS 30.0%
CTTCTTGTCATCAATTACTT+CGG + Chr6:5293943-5293962 None:intergenic 30.0%
GAAAGAGGTTAAGTTATCAA+AGG - Chr6:5294950-5294969 MsG0680030561.01.T01:intron 30.0%
GCTAAGATAAGCAAAATTTC+AGG - Chr6:5293830-5293849 MsG0680030561.01.T01:CDS 30.0%
TACTTTGACAATTTGTGTGT+TGG - Chr6:5294617-5294636 MsG0680030561.01.T01:intron 30.0%
TGCAAAATTTCACAAGGATT+TGG - Chr6:5294854-5294873 MsG0680030561.01.T01:intron 30.0%
TTTGAATTCACAAGAGATGA+GGG - Chr6:5294201-5294220 MsG0680030561.01.T01:intron 30.0%
! AGGGTATCAAAATATTGTCA+AGG - Chr6:5293961-5293980 MsG0680030561.01.T01:CDS 30.0%
! CTTTGTTTCTTTGATGTGTT+GGG - Chr6:5293906-5293925 MsG0680030561.01.T01:CDS 30.0%
! GAAGTAATTGATGACAAGAA+GGG - Chr6:5293942-5293961 MsG0680030561.01.T01:CDS 30.0%
!! AGCACTGTATTTTTGAAGAA+GGG - Chr6:5294153-5294172 MsG0680030561.01.T01:CDS 30.0%
!! TAGCACTGTATTTTTGAAGA+AGG - Chr6:5294152-5294171 MsG0680030561.01.T01:CDS 30.0%
!! TTCATAATGGATTTAGTGCA+TGG - Chr6:5294066-5294085 MsG0680030561.01.T01:CDS 30.0%
AAAGCAAATGCTGTTGAAAG+AGG - Chr6:5294935-5294954 MsG0680030561.01.T01:intron 35.0%
AATATTGTCAAGGAAGGAGA+AGG - Chr6:5293971-5293990 MsG0680030561.01.T01:CDS 35.0%
CTGAGCATGAAACAGAAAAA+TGG - Chr6:5294988-5295007 MsG0680030561.01.T01:intron 35.0%
GATGGTTCAGAAATTCACTT+TGG - Chr6:5294827-5294846 MsG0680030561.01.T01:intron 35.0%
GGAGATTGCAAAATTTCACA+AGG - Chr6:5294848-5294867 MsG0680030561.01.T01:intron 35.0%
GTTTGAATTCACAAGAGATG+AGG - Chr6:5294200-5294219 MsG0680030561.01.T01:intron 35.0%
TATGCATTTGGATGCTACAA+AGG - Chr6:5294761-5294780 MsG0680030561.01.T01:intron 35.0%
TTTACAACAGCTCCATCATT+TGG + Chr6:5294007-5294026 None:intergenic 35.0%
! AGGCTCTCTTCTTTTCATAA+TGG - Chr6:5294053-5294072 MsG0680030561.01.T01:CDS 35.0%
! CGAAGTAATTGATGACAAGA+AGG - Chr6:5293941-5293960 MsG0680030561.01.T01:CDS 35.0%
!! AAACATTGACTTTCGTCAGT+TGG + Chr6:5294282-5294301 None:intergenic 35.0%
!! GCTTTGTTTCTTTGATGTGT+TGG - Chr6:5293905-5293924 MsG0680030561.01.T01:CDS 35.0%
AAATGGAGAGAAGCTTCAGA+AGG - Chr6:5295005-5295024 MsG0680030561.01.T01:intron 40.0%
AAGAGATGGTACTTCGAACT+TGG + Chr6:5294475-5294494 None:intergenic 40.0%
AAGTACCATCTCTTCAACAC+TGG - Chr6:5294481-5294500 MsG0680030561.01.T01:intron 40.0%
AGCTGTAATGACCATGAAGA+AGG - Chr6:5294363-5294382 MsG0680030561.01.T01:intron 40.0%
CACTGCAAAGATGCAAAATG+AGG + Chr6:5294109-5294128 None:intergenic 40.0%
GCTGTAATGACCATGAAGAA+GGG - Chr6:5294364-5294383 MsG0680030561.01.T01:intron 40.0%
TTCTGTCATGGAGAACAAAG+AGG - Chr6:5294794-5294813 MsG0680030561.01.T01:intron 40.0%
! CAAAGAGGAGTCTGATTTGA+TGG - Chr6:5294809-5294828 MsG0680030561.01.T01:intron 40.0%
!! GGAGGAACTTATTTCTGTCA+TGG - Chr6:5294782-5294801 MsG0680030561.01.T01:intron 40.0%
AATGACCATGAAGAAGGGTG+AGG - Chr6:5294369-5294388 MsG0680030561.01.T01:intron 45.0%
AGACATCTTGCGACAGAACT+CGG - Chr6:5294729-5294748 MsG0680030561.01.T01:CDS 45.0%
GAGCAGAGCAAGTGATTGAT+GGG - Chr6:5294332-5294351 MsG0680030561.01.T01:intron 45.0%
GCATTTGGATGCTACAAAGG+AGG - Chr6:5294764-5294783 MsG0680030561.01.T01:intron 45.0%
GGATATGAGCGTCCAAATGA+TGG - Chr6:5293992-5294011 MsG0680030561.01.T01:CDS 45.0%
GTTGTATTGAGCAGAGCAAG+TGG - Chr6:5294302-5294321 MsG0680030561.01.T01:intron 45.0%
TACCATCTCTTCAACACTGG+TGG - Chr6:5294484-5294503 MsG0680030561.01.T01:intron 45.0%
TGAGCAGAGCAAGTGATTGA+TGG - Chr6:5294331-5294350 MsG0680030561.01.T01:intron 45.0%
!! GTATTTTTGAAGAAGGGCCG+TGG - Chr6:5294159-5294178 MsG0680030561.01.T01:CDS 45.0%
CGGTCGCACTGTTATGCATT+TGG - Chr6:5294749-5294768 MsG0680030561.01.T01:CDS 50.0%
GCTCTGTTTCATCATCGCCA+CGG + Chr6:5294179-5294198 None:intergenic 50.0%
TGCTACCTCACCCTTCTTCA+TGG + Chr6:5294377-5294396 None:intergenic 50.0%
! GTCCACCAGTGTTGAAGAGA+TGG + Chr6:5294489-5294508 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 5293821 5295079 5293821 ID=MsG0680030561.01;Name=MsG0680030561.01
Chr6 mRNA 5293821 5295079 5293821 ID=MsG0680030561.01.T01;Parent=MsG0680030561.01;Name=MsG0680030561.01.T01;_AED=0.35;_eAED=0.36;_QI=0|0.77|0.8|1|0.33|0.3|10|0|196
Chr6 exon 5293821 5294211 5293821 ID=MsG0680030561.01.T01:exon:4372;Parent=MsG0680030561.01.T01
Chr6 exon 5294490 5294562 5294490 ID=MsG0680030561.01.T01:exon:4371;Parent=MsG0680030561.01.T01
Chr6 exon 5294678 5294773 5294678 ID=MsG0680030561.01.T01:exon:4370;Parent=MsG0680030561.01.T01
Chr6 exon 5295049 5295079 5295049 ID=MsG0680030561.01.T01:exon:4369;Parent=MsG0680030561.01.T01
Chr6 CDS 5295049 5295079 5295049 ID=MsG0680030561.01.T01:cds;Parent=MsG0680030561.01.T01
Chr6 CDS 5294678 5294773 5294678 ID=MsG0680030561.01.T01:cds;Parent=MsG0680030561.01.T01
Chr6 CDS 5294490 5294562 5294490 ID=MsG0680030561.01.T01:cds;Parent=MsG0680030561.01.T01
Chr6 CDS 5293821 5294211 5293821 ID=MsG0680030561.01.T01:cds;Parent=MsG0680030561.01.T01
Gene Sequence

>MsG0680030561.01.T01

ATGCAAGGTGCTAAGATAAGCAAAATTTCAGTGAAAGTAATTGGTAAGTTGCAAGATAGCACTGTATTTTTGAAGAAGGGCCGTGGCGATGATGAAACAGAGCTGTTTGAATTCACAAGAGATGAGGAGCAAGTGATTGATGGGCTCGATAGAGCTGTAATGACCATGAAGAAGGGTGAGGTAGCATTGTTGACTATTGCGAAACACAAAACTGCTTTCATTGAGCTGATTTCAAATCAAAGACATCTTGCGACAGAACTCGGTCGCACTGTTATGCATTTGGATGCTACAAAGGAGGAACTTATTTCTGTCATGGAGAACAAAGAGGAGTCTGATTTGATGGTTCAGAAATTCACTTTGGAGATTGCAAAATTTCACAAGGATTTGGAACAAAAAGATAAAATTTTGTCAGCAATGTTGAAGAAATCTAAATTTGATACTGCAGAAAAGCAAATGCTGTTGAAAGAGGTTAAGTTATCAAAGGTTAGAAGGAAGCATGCTGAGCATGAAACAGAAAAATGGAGAGAAGCTTCAGAAGGAAAACATGACAGACATTGCTTTAAAAACATGTTGATGCACACATTCCTTTAA

Protein sequence

>MsG0680030561.01.T01

MQGAKISKISVKVIGKLQDSTVFLKKGRGDDETELFEFTRDEEQVIDGLDRAVMTMKKGEVALLTIAKHKTAFIELISNQRHLATELGRTVMHLDATKEELISVMENKEESDLMVQKFTLEIAKFHKDLEQKDKILSAMLKKSKFDTAEKQMLLKEVKLSKVRRKHAEHETEKWREASEGKHDRHCFKNMLMHTFL*