Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031115.01.T01 | XP_007151450.1 | 68.579 | 401 | 42 | 5 | 73 | 442 | 295 | 642 | 7.01E-179 | 525 |
| MsG0680031115.01.T01 | XP_007151450.1 | 62.637 | 91 | 32 | 1 | 1 | 91 | 130 | 218 | 5.49E-24 | 117 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0680031115.01 | 0.818300 | 2.106280e-52 | 1.570622e-49 |
| MsG0080047945.01 | MsG0680031115.01 | 0.810489 | 1.124005e-50 | 6.784421e-48 |
| MsG0080047955.01 | MsG0680031115.01 | 0.808052 | 3.742622e-50 | 2.118258e-47 |
| MsG0080048068.01 | MsG0680031115.01 | 0.818589 | 1.811245e-52 | 1.361426e-49 |
| MsG0080048198.01 | MsG0680031115.01 | 0.806525 | 7.885308e-50 | 4.289425e-47 |
| MsG0080048528.01 | MsG0680031115.01 | 0.831960 | 1.241364e-55 | 1.368170e-52 |
| MsG0080048773.01 | MsG0680031115.01 | 0.853476 | 2.330276e-61 | 5.041405e-58 |
| MsG0080048777.01 | MsG0680031115.01 | 0.840244 | 9.770862e-58 | 1.383491e-54 |
| MsG0080048780.01 | MsG0680031115.01 | 0.872324 | 3.470354e-67 | 1.445897e-63 |
| MsG0080048801.01 | MsG0680031115.01 | 0.865496 | 5.660719e-65 | 1.845448e-61 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031115.01.T01 | MTR_6g016600 | 68.342 | 398 | 40 | 6 | 77 | 442 | 266 | 609 | 2.89e-175 | 504 |
| MsG0680031115.01.T01 | MTR_6g016600 | 93.506 | 77 | 5 | 0 | 1 | 77 | 104 | 180 | 6.08e-37 | 143 |
| MsG0680031115.01.T01 | MTR_7g096060 | 46.919 | 211 | 89 | 1 | 188 | 375 | 248 | 458 | 2.94e-60 | 209 |
| MsG0680031115.01.T01 | MTR_7g010800 | 54.412 | 204 | 69 | 2 | 195 | 376 | 221 | 422 | 9.72e-60 | 207 |
| MsG0680031115.01.T01 | MTR_3g115110 | 46.512 | 258 | 100 | 4 | 192 | 416 | 261 | 513 | 1.06e-59 | 207 |
| MsG0680031115.01.T01 | MTR_5g069780 | 48.249 | 257 | 93 | 6 | 181 | 399 | 312 | 566 | 1.75e-58 | 205 |
| MsG0680031115.01.T01 | MTR_3g104470 | 52.475 | 202 | 75 | 2 | 194 | 376 | 238 | 437 | 3.21e-58 | 203 |
| MsG0680031115.01.T01 | MTR_4g094662 | 46.559 | 247 | 101 | 4 | 182 | 404 | 229 | 468 | 4.34e-58 | 202 |
| MsG0680031115.01.T01 | MTR_3g104480 | 53.234 | 201 | 73 | 2 | 195 | 376 | 237 | 435 | 5.75e-58 | 202 |
| MsG0680031115.01.T01 | MTR_2g438140 | 46.154 | 247 | 102 | 4 | 182 | 404 | 226 | 465 | 6.11e-58 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031115.01.T01 | AT3G02450 | 57.286 | 398 | 82 | 7 | 77 | 441 | 280 | 622 | 5.30e-141 | 417 |
| MsG0680031115.01.T01 | AT3G02450 | 45.205 | 73 | 36 | 1 | 5 | 77 | 130 | 198 | 1.27e-12 | 70.1 |
| MsG0680031115.01.T01 | AT2G26140 | 53.960 | 202 | 72 | 2 | 194 | 376 | 238 | 437 | 8.80e-60 | 207 |
| MsG0680031115.01.T01 | AT1G50250 | 53.140 | 207 | 73 | 2 | 192 | 376 | 271 | 475 | 1.73e-59 | 207 |
| MsG0680031115.01.T01 | AT5G42270 | 50.685 | 219 | 82 | 3 | 182 | 376 | 247 | 463 | 5.16e-59 | 205 |
| MsG0680031115.01.T01 | AT3G47060 | 36.386 | 404 | 200 | 9 | 54 | 402 | 177 | 578 | 6.52e-59 | 206 |
| MsG0680031115.01.T01 | AT5G58870 | 47.308 | 260 | 97 | 6 | 181 | 402 | 325 | 582 | 1.49e-58 | 206 |
| MsG0680031115.01.T01 | AT5G15250 | 54.229 | 201 | 68 | 2 | 198 | 376 | 239 | 437 | 1.69e-58 | 204 |
| MsG0680031115.01.T01 | AT5G53170 | 51.208 | 207 | 81 | 2 | 188 | 376 | 367 | 571 | 4.26e-58 | 204 |
| MsG0680031115.01.T01 | AT2G30950 | 46.559 | 247 | 101 | 4 | 182 | 404 | 224 | 463 | 4.33e-58 | 202 |
Find 117 sgRNAs with CRISPR-Local
Find 289 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTAAAGTTACGGTGGC+AGG | 0.467929 | 6:-14697343 | None:intergenic |
| AAACATTTCAACGAACTCAC+TGG | 0.559253 | 6:-14698380 | None:intergenic |
| AAACCTAATAGTCAGACAGT+TGG | 0.591387 | 6:+14696282 | MsG0680031115.01.T01:CDS |
| AACTAACCGGCCAGAAGCCT+TGG | 0.480710 | 6:+14699160 | MsG0680031115.01.T01:CDS |
| AAGGTCTAGTAAAATAAGCA+AGG | 0.598431 | 6:+14700282 | MsG0680031115.01.T01:CDS |
| AATCCAACTGTCTGACTATT+AGG | 0.199018 | 6:-14696285 | None:intergenic |
| AATCTGTGTTAGCGGCGATG+AGG | 0.652895 | 6:+14696160 | MsG0680031115.01.T01:CDS |
| AATGTCTTGCCAGTTCCTGG+AGG | 0.719587 | 6:-14698304 | None:intergenic |
| ACAGTTACTGATGGTCTCGT+AGG | 0.621604 | 6:+14699329 | MsG0680031115.01.T01:CDS |
| ACGACACAGGGCTGGATCCA+AGG | 0.506054 | 6:-14699177 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTGAATCTCATAAAACAG+GGG | - | Chr6:14697229-14697248 | None:intergenic | 30.0% | |
| AAGGTCTAGTAAAATAAGCA+AGG | + | Chr6:14700282-14700301 | MsG0680031115.01.T01:CDS | 30.0% | |
| AAGTAAATCATATATTCCCG+AGG | + | Chr6:14696880-14696899 | MsG0680031115.01.T01:intron | 30.0% | |
| AGAAAGTTCAACTAACTTCT+TGG | + | Chr6:14696681-14696700 | MsG0680031115.01.T01:intron | 30.0% | |
| ATGGATAAGGATATAGACAA+GGG | + | Chr6:14695958-14695977 | MsG0680031115.01.T01:intron | 30.0% | |
| ATTCATTGATGAACTTGATG+CGG | + | Chr6:14698471-14698490 | MsG0680031115.01.T01:CDS | 30.0% | |
| ATTTGAATCAGAAATGAGAG+TGG | + | Chr6:14699124-14699143 | MsG0680031115.01.T01:CDS | 30.0% | |
| CAATCTATAGCAGTTTGAAT+TGG | - | Chr6:14697821-14697840 | None:intergenic | 30.0% | |
| CAATTCAAACTGCTATAGAT+TGG | + | Chr6:14697819-14697838 | MsG0680031115.01.T01:intron | 30.0% | |
| CATCTTTGTATGTTACCAAA+AGG | - | Chr6:14696560-14696579 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 14695638 | 14700408 | 14695638 | ID=MsG0680031115.01;Name=MsG0680031115.01 |
| Chr6 | mRNA | 14695638 | 14700408 | 14695638 | ID=MsG0680031115.01.T01;Parent=MsG0680031115.01;Name=MsG0680031115.01.T01;_AED=0.49;_eAED=0.56;_QI=0|0|0|1|1|1|6|0|442 |
| Chr6 | exon | 14695638 | 14695864 | 14695638 | ID=MsG0680031115.01.T01:exon:9998;Parent=MsG0680031115.01.T01 |
| Chr6 | exon | 14696149 | 14696353 | 14696149 | ID=MsG0680031115.01.T01:exon:9999;Parent=MsG0680031115.01.T01 |
| Chr6 | exon | 14697247 | 14697405 | 14697247 | ID=MsG0680031115.01.T01:exon:10000;Parent=MsG0680031115.01.T01 |
| Chr6 | exon | 14698226 | 14698492 | 14698226 | ID=MsG0680031115.01.T01:exon:10001;Parent=MsG0680031115.01.T01 |
| Chr6 | exon | 14699104 | 14699370 | 14699104 | ID=MsG0680031115.01.T01:exon:10002;Parent=MsG0680031115.01.T01 |
| Chr6 | exon | 14700205 | 14700408 | 14700205 | ID=MsG0680031115.01.T01:exon:10003;Parent=MsG0680031115.01.T01 |
| Chr6 | CDS | 14695638 | 14695864 | 14695638 | ID=MsG0680031115.01.T01:cds;Parent=MsG0680031115.01.T01 |
| Chr6 | CDS | 14696149 | 14696353 | 14696149 | ID=MsG0680031115.01.T01:cds;Parent=MsG0680031115.01.T01 |
| Chr6 | CDS | 14697247 | 14697405 | 14697247 | ID=MsG0680031115.01.T01:cds;Parent=MsG0680031115.01.T01 |
| Chr6 | CDS | 14698226 | 14698492 | 14698226 | ID=MsG0680031115.01.T01:cds;Parent=MsG0680031115.01.T01 |
| Chr6 | CDS | 14699104 | 14699370 | 14699104 | ID=MsG0680031115.01.T01:cds;Parent=MsG0680031115.01.T01 |
| Chr6 | CDS | 14700205 | 14700408 | 14700205 | ID=MsG0680031115.01.T01:cds;Parent=MsG0680031115.01.T01 |
| Gene Sequence |
>MsG0680031115.01.T01 ATGAAGATGAAAAGGGCTTCTGTTGAATCTGTTTTAAATGAGGTTGAAGTGTTTGTTAGGAAGAATATTAGGACGGTGGCGTTTGCTACATCGTTTTCGATTGTGTTTACACTTTGTTTTTTGTTTTTGAAGTTTACTTCACTTCGGCCAGTGAAAATTGTTCCGTATTCAGACTTGATTGCGAGTCTTCGAAATGGGAATGTGGAGAAAGTTTTAGTTGAAGAGGGCTCGGCTCCTCAATCTGTGTTAGCGGCGATGAGGAGTACTTTGATAACTGTGATCACACTGTGGATACCTTTGATTCCTTTGATGTGGCTTATATATCGTCAACTTTCTGCTGCTAGTCCAGCTAAGAAGCGGAAACCTAATAGTCAGACAGTTGGATTTGAAGATGTTCAAGGTGTTGATTCTGCAAAAGTAGAGCTCATGGAGTTTAATCATTATGCTTCGGCCAACAACAACCCAATGGGAAACACAGCCAAGTGGCCCACCTTAAGGCTGGCCTCTTGGAGTGTTTTGCCCATCTGTCCTGCCACCGTAACTTTAATTTTTGACCATTTGTGTACTGCTACGCAACTTAAGTTGCGTACTATAGTTTCATGTCTGCAAGGGGATCTTAATTACCAAAAGGTAGGGGCAAAGCTACCTAGAGGAGTGTTGCTTGTCGGTCCTCCAGGAACTGGCAAGACATTACTTGCACGTGCAATAGCGGGGGAAGCAGGTGTACCCTTTTTCACAGTATCTGCCAGTGAGTTCGTTGAAATGTTTGTTGGAAGAGGTGCTGCTAGAATCAGAGACCTTTTTAGTACAGCAAGGAAATTTGCACCATCAATCATATTCATTGATGAACTTGATGCGTTGCTGACCGAAATGGATGGATTTGAATCAGAAATGAGAGTGGTTGTCATTGCAGCAACTAACCGGCCAGAAGCCTTGGATCCAGCCCTGTGTCGTCCCGGTCGTTTCTCGAGGAAAGTATTTGTAGGGGAACCTGACGAAGAAGGAAGGAGAAAGATATTAGCTGTGCATTTGAGAGGAGTTCCTTTGGAGGAGGATGCTAACATCATATGTCATTTAATTGCCACAGTTACTGATGGTCTCGTAGGTGCTGATCTTGCAAACATCGTAGTGAAACTGTGGCAAGGGAAGAATATAATGGAAGCTATTGAAAGAGCAAAATTTGGAATCAATGATAAGCAACTAAGGTCTAGTAAAATAAGCAAGGAGCTTGGTAAGCTGTTCCCTTGGATGCCATCATTGATGGGAAGAAGTGATAGGAAACAAGATGACATACAAGGACCATTAGGTTATCAATCATTGAATTCATGA |
| Protein sequence |
>MsG0680031115.01.T01 MKMKRASVESVLNEVEVFVRKNIRTVAFATSFSIVFTLCFLFLKFTSLRPVKIVPYSDLIASLRNGNVEKVLVEEGSAPQSVLAAMRSTLITVITLWIPLIPLMWLIYRQLSAASPAKKRKPNSQTVGFEDVQGVDSAKVELMEFNHYASANNNPMGNTAKWPTLRLASWSVLPICPATVTLIFDHLCTATQLKLRTIVSCLQGDLNYQKVGAKLPRGVLLVGPPGTGKTLLARAIAGEAGVPFFTVSASEFVEMFVGRGAARIRDLFSTARKFAPSIIFIDELDALLTEMDGFESEMRVVVIAATNRPEALDPALCRPGRFSRKVFVGEPDEEGRRKILAVHLRGVPLEEDANIICHLIATVTDGLVGADLANIVVKLWQGKNIMEAIERAKFGINDKQLRSSKISKELGKLFPWMPSLMGRSDRKQDDIQGPLGYQSLNS* |
