AlfalfaGEDB | MsG0680031241.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031241.01

KEGG: -
GO: GO:0005515
PFAM: PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031241.01.T01	XP_003618770.1	96.053	304	12	0	1	304	1	304	0	612

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031241.01.T01	Q8VYR5	54.098	305	133	3	1	304	1	299	8.10E-121	357

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280007752.01	MsG0680031241.01	0.803469	3.434085e-49	1.727098e-46
MsG0480021424.01	MsG0680031241.01	0.801954	7.056718e-49	3.414237e-46
MsG0480021811.01	MsG0680031241.01	0.815037	1.134970e-51	7.739743e-49
MsG0480021984.01	MsG0680031241.01	0.807533	4.824918e-50	2.694040e-47
MsG0480021985.01	MsG0680031241.01	0.804403	2.196951e-49	1.131691e-46
MsG0680031241.01	MsG0680031242.01	0.848030	7.947687e-60	1.439230e-56
MsG0680031241.01	MsG0780038824.01	0.814397	1.572928e-51	1.054306e-48
MsG0680031241.01	MsG0780040949.01	0.836959	6.891429e-57	8.822079e-54
MsG0680031241.01	MsG0880043077.01	0.823103	1.658621e-53	1.414745e-50
MsG0680031241.01	MsG0880046844.01	0.807822	4.188838e-50	2.356528e-47

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PPI

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Gene1	Gene2	Type
MsG0780039369.01	MsG0680031241.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031241.01.T01	MTR_6g021740	96.053	304	12	0	1	304	1	304	0.0	612
MsG0680031241.01.T01	MTR_2g437460	30.000	150	105	0	43	192	202	351	6.48e-17	81.6
MsG0680031241.01.T01	MTR_1g033960	29.530	149	105	0	44	192	263	411	1.48e-14	74.3
MsG0680031241.01.T01	MTR_1g033960	25.556	180	111	5	38	196	147	324	2.02e-11	65.1
MsG0680031241.01.T01	MTR_3g105900	26.174	149	110	0	48	196	939	1087	1.87e-14	74.3
MsG0680031241.01.T01	MTR_3g105900	24.454	229	150	4	67	288	222	434	4.47e-11	63.9
MsG0680031241.01.T01	MTR_2g026645	26.275	255	174	7	44	294	286	530	2.60e-14	73.6
MsG0680031241.01.T01	MTR_8g105170	26.829	164	116	2	34	195	371	532	3.66e-14	73.6
MsG0680031241.01.T01	MTR_1g068960	28.571	161	115	0	34	194	436	596	7.89e-14	72.4
MsG0680031241.01.T01	MTR_5g095130	24.521	261	175	4	44	304	621	859	9.22e-14	72.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031241.01.T01	AT4G35850	54.098	305	133	3	1	304	1	299	8.26e-122	357
MsG0680031241.01.T01	AT2G16880	26.241	141	104	0	48	188	243	383	1.28e-13	71.6
MsG0680031241.01.T01	AT3G49730	24.176	182	132	2	38	218	229	405	1.45e-13	71.6
MsG0680031241.01.T01	AT2G37230	26.000	150	111	0	43	192	218	367	1.47e-13	71.6
MsG0680031241.01.T01	AT5G61990	24.627	268	188	4	35	298	407	664	4.16e-13	70.1
MsG0680031241.01.T01	AT4G16390	27.500	160	115	1	34	192	304	463	5.02e-13	69.7
MsG0680031241.01.T01	AT2G02150	29.114	158	112	0	48	205	265	422	5.94e-13	69.7
MsG0680031241.01.T01	AT2G02150	29.114	158	112	0	48	205	265	422	5.94e-13	69.7
MsG0680031241.01.T01	AT4G19440	27.703	148	101	2	48	192	556	700	1.38e-12	68.6
MsG0680031241.01.T01	AT4G19440	24.880	209	136	2	16	207	616	820	5.61e-11	63.9

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Find 100 sgRNAs with CRISPR-Local

Find 412 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTTCTTCAATCTCTCTT+AGG	0.270939	6:+16549484	MsG0680031241.01.T01:CDS
AACTTTCTAATTTCAACCTG+TGG	0.505817	6:+16552742	MsG0680031241.01.T01:CDS
AAGGACCAAATGAAAATCAT+GGG	0.535637	6:+16552113	MsG0680031241.01.T01:CDS
AAGGGAATGAAAAGTTTCAC+GGG	0.575462	6:-16552042	None:intergenic
AATCGGAGCCCCGAAACCCG+TGG	0.634595	6:-16549624	None:intergenic
AATGCTGAGAATGTGATGGT+GGG	0.697576	6:+16553588	MsG0680031241.01.T01:CDS
ACACTCGGTCTATGCGTCCT+TGG	0.391054	6:-16552985	None:intergenic
ACATGTCTTAGTGTTACAAA+TGG	0.505318	6:+16555839	MsG0680031241.01.T01:three_prime_UTR
ACATGTTAATACCTAGACAA+TGG	0.675595	6:-16555822	None:intergenic
ACCCATTTCGCACTGCAAAA+CGG	0.485063	6:-16549596	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATGGCATGTTTCCTTT+AGG	-	Chr6:16553068-16553087	None:intergenic	30.0%
AAACTACGCTAAAAATGTTC+AGG	+	Chr6:16549680-16549699	MsG0680031241.01.T01:CDS	30.0%
AAAGGCTAAACATAGAAGAA+AGG	-	Chr6:16554441-16554460	None:intergenic	30.0%
AACTTTCTAATTTCAACCTG+TGG	+	Chr6:16552742-16552761	MsG0680031241.01.T01:CDS	30.0%
AAGAAATGTGAAAAAGGCAA+AGG	-	Chr6:16554593-16554612	None:intergenic	30.0%
AAGGACCAAATGAAAATCAT+GGG	+	Chr6:16552113-16552132	MsG0680031241.01.T01:CDS	30.0%
AATAACCACCATATTTAGCA+TGG	-	Chr6:16552446-16552465	None:intergenic	30.0%
ACATAAAATATGTAAGGTCC+GGG	+	Chr6:16550735-16550754	MsG0680031241.01.T01:intron	30.0%
ACATGTCTTAGTGTTACAAA+TGG	+	Chr6:16555839-16555858	MsG0680031241.01.T01:three_prime_UTR	30.0%
ACATGTTAATACCTAGACAA+TGG	-	Chr6:16555825-16555844	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	16549416	16556465	16549416	ID=MsG0680031241.01;Name=MsG0680031241.01
Chr6	mRNA	16549416	16556465	16549416	ID=MsG0680031241.01.T01;Parent=MsG0680031241.01;Name=MsG0680031241.01.T01;_AED=0.46;_eAED=0.46;_QI=65\|1\|1\|1\|0.85\|0.87\|8\|839\|304
Chr6	exon	16549416	16549505	16549416	ID=MsG0680031241.01.T01:exon:4461;Parent=MsG0680031241.01.T01
Chr6	exon	16549589	16549742	16549589	ID=MsG0680031241.01.T01:exon:4462;Parent=MsG0680031241.01.T01
Chr6	exon	16551986	16552148	16551986	ID=MsG0680031241.01.T01:exon:4463;Parent=MsG0680031241.01.T01
Chr6	exon	16552730	16552795	16552730	ID=MsG0680031241.01.T01:exon:4464;Parent=MsG0680031241.01.T01
Chr6	exon	16552908	16553005	16552908	ID=MsG0680031241.01.T01:exon:4465;Parent=MsG0680031241.01.T01
Chr6	exon	16553328	16553445	16553328	ID=MsG0680031241.01.T01:exon:4466;Parent=MsG0680031241.01.T01
Chr6	exon	16553531	16553740	16553531	ID=MsG0680031241.01.T01:exon:4467;Parent=MsG0680031241.01.T01
Chr6	exon	16555546	16556465	16555546	ID=MsG0680031241.01.T01:exon:4468;Parent=MsG0680031241.01.T01
Chr6	five_prime_UTR	16549416	16549480	16549416	ID=MsG0680031241.01.T01:five_prime_utr;Parent=MsG0680031241.01.T01
Chr6	CDS	16549481	16549505	16549481	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16549589	16549742	16549589	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16551986	16552148	16551986	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16552730	16552795	16552730	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16552908	16553005	16552908	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16553328	16553445	16553328	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16553531	16553740	16553531	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	CDS	16555546	16555626	16555546	ID=MsG0680031241.01.T01:cds;Parent=MsG0680031241.01.T01
Chr6	three_prime_UTR	16555627	16556465	16555627	ID=MsG0680031241.01.T01:three_prime_utr;Parent=MsG0680031241.01.T01

Gene Sequence

>MsG0680031241.01.T01

ATGAAATTTCTTCAATCTCTCTTAGGTAAGACCCGTTTTGCAGTGCGAAATGGGTCACTTCCACGGGTTTCGGGGCTCCGATTCTACAATGTTCCAGCAGAAGAGTATAACAGGAGAAACTACGCTAAAAATGTTCAGGAATACAACACTGTTTTGGGTTCTCTCAATGCTCAACGAAGGATTTTTTTGCTGAGAGATGTGTATGAAGATATGATGCTTGATGGAGTGAGGCCAACCCGTGAAACTTTTCATTCCCTTGCTGTTGGATCCATGAGAGGCTCTAGAATGCATGATGCTTTCTTCTTCAAGGACCAAATGAAAATCATGGGTTTGGTTCCTGATGTTACTTTCTACAACTTTCTAATTTCAACCTGTGGGAAATGCAAGAACTCTGATCAGGCTGTTCAGATTTTGGAGGAGATGAAATCCATGGAAGTAAAGCCAAATGTACAAACCTACATCTGCTTGCTACATGCATGTGCAGCCCAAGGACGCATAGACCGAGTGCATGCAATTGTCCGTGATATGACCGCAGCTGGTCTGGGATTAAATAAGTTCTGCTATGCTGGGCTTATAGTTGCACACCAGAACAAAACACCTTTTACTGATGATTTCGATGCAAAAGTTATTGAGTATGTGGAGAGGTCAAAGCAATGGACTTCAGTAGAAACTGACAGCGCCAATGCTGAGAATGTGATGGTGGGTGTTACTGATGAGGAGTTATATAACTTACCAACGGCAGAATACGTTACTAGGCGTGGAGGATTCTTGAACAGGCCCTTTGTTGCATACCATACTGCATTTAATGCTGCTGCGGATTTCAAGAATGTTAAGTTGACGGATGCACTTCTTGAAATGCTGGAAAAGGAGAAGAAAACCCCTGATATCTATATTGTCATGCAAGTAATTAGGTAA

Protein sequence

>MsG0680031241.01.T01

MKFLQSLLGKTRFAVRNGSLPRVSGLRFYNVPAEEYNRRNYAKNVQEYNTVLGSLNAQRRIFLLRDVYEDMMLDGVRPTRETFHSLAVGSMRGSRMHDAFFFKDQMKIMGLVPDVTFYNFLISTCGKCKNSDQAVQILEEMKSMEVKPNVQTYICLLHACAAQGRIDRVHAIVRDMTAAGLGLNKFCYAGLIVAHQNKTPFTDDFDAKVIEYVERSKQWTSVETDSANAENVMVGVTDEELYNLPTAEYVTRRGGFLNRPFVAYHTAFNAAADFKNVKLTDALLEMLEKEKKTPDIYIVMQVIR*