Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039369.01.T01 | XP_024625347.1 | 95.733 | 375 | 15 | 1 | 63 | 436 | 116 | 490 | 0 | 707 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048065.01 | MsG0780039369.01 | 0.826665 | 2.395501e-54 | 2.261122e-51 |
| MsG0080048152.01 | MsG0780039369.01 | 0.836731 | 7.878413e-57 | 1.001650e-53 |
| MsG0080048307.01 | MsG0780039369.01 | 0.819789 | 9.657995e-53 | 7.505565e-50 |
| MsG0080048787.01 | MsG0780039369.01 | 0.818180 | 2.242926e-52 | 1.666988e-49 |
| MsG0080048878.01 | MsG0780039369.01 | 0.816626 | 5.017772e-52 | 3.573840e-49 |
| MsG0080048892.01 | MsG0780039369.01 | 0.810222 | 1.283198e-50 | 7.690683e-48 |
| MsG0180000114.01 | MsG0780039369.01 | 0.801309 | 9.569346e-49 | 4.555130e-46 |
| MsG0180000218.01 | MsG0780039369.01 | -0.854951 | 8.741773e-62 | 1.985712e-58 |
| MsG0180000354.01 | MsG0780039369.01 | 0.802549 | 5.321705e-49 | 2.614191e-46 |
| MsG0180000355.01 | MsG0780039369.01 | 0.830102 | 3.546979e-55 | 3.700811e-52 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039369.01.T01 | MTR_7g072070 | 95.733 | 375 | 15 | 1 | 63 | 436 | 116 | 490 | 0.0 | 707 |
| MsG0780039369.01.T01 | MTR_8g105170 | 30.435 | 345 | 237 | 2 | 74 | 418 | 223 | 564 | 7.49e-41 | 155 |
| MsG0780039369.01.T01 | MTR_8g105170 | 27.019 | 322 | 232 | 2 | 97 | 418 | 316 | 634 | 7.24e-36 | 141 |
| MsG0780039369.01.T01 | MTR_8g105170 | 22.289 | 332 | 249 | 3 | 74 | 402 | 363 | 688 | 4.95e-25 | 108 |
| MsG0780039369.01.T01 | MTR_6g079180 | 27.485 | 342 | 245 | 2 | 77 | 418 | 26 | 364 | 1.47e-40 | 149 |
| MsG0780039369.01.T01 | MTR_6g079180 | 25.152 | 330 | 234 | 4 | 73 | 397 | 57 | 378 | 3.16e-30 | 120 |
| MsG0780039369.01.T01 | MTR_6g079180 | 26.255 | 259 | 156 | 1 | 195 | 418 | 1 | 259 | 1.53e-24 | 104 |
| MsG0780039369.01.T01 | MTR_6g079210 | 27.485 | 342 | 245 | 2 | 77 | 418 | 26 | 364 | 1.54e-40 | 149 |
| MsG0780039369.01.T01 | MTR_6g079210 | 25.155 | 322 | 228 | 4 | 73 | 389 | 57 | 370 | 8.68e-31 | 122 |
| MsG0780039369.01.T01 | MTR_6g079210 | 26.255 | 259 | 156 | 1 | 195 | 418 | 1 | 259 | 7.57e-25 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039369.01.T01 | AT2G38420 | 46.988 | 332 | 176 | 0 | 68 | 399 | 118 | 449 | 4.52e-102 | 311 |
| MsG0780039369.01.T01 | AT1G05670 | 31.359 | 287 | 195 | 1 | 132 | 418 | 354 | 638 | 2.66e-41 | 156 |
| MsG0780039369.01.T01 | AT1G05670 | 26.823 | 384 | 237 | 4 | 73 | 418 | 226 | 603 | 5.55e-35 | 138 |
| MsG0780039369.01.T01 | AT1G05670 | 24.740 | 384 | 268 | 7 | 56 | 434 | 347 | 714 | 4.05e-28 | 117 |
| MsG0780039369.01.T01 | AT1G05670 | 25.228 | 329 | 235 | 6 | 108 | 432 | 120 | 441 | 1.63e-21 | 97.8 |
| MsG0780039369.01.T01 | AT1G05670 | 31.359 | 287 | 195 | 1 | 132 | 418 | 354 | 638 | 2.66e-41 | 156 |
| MsG0780039369.01.T01 | AT1G05670 | 26.823 | 384 | 237 | 4 | 73 | 418 | 226 | 603 | 5.55e-35 | 138 |
| MsG0780039369.01.T01 | AT1G05670 | 24.740 | 384 | 268 | 7 | 56 | 434 | 347 | 714 | 4.05e-28 | 117 |
| MsG0780039369.01.T01 | AT1G05670 | 25.228 | 329 | 235 | 6 | 108 | 432 | 120 | 441 | 1.63e-21 | 97.8 |
| MsG0780039369.01.T01 | AT1G05670 | 31.359 | 287 | 195 | 1 | 132 | 418 | 354 | 638 | 2.66e-41 | 156 |
Find 77 sgRNAs with CRISPR-Local
Find 217 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGATTTGACTAGCGTCG+AGG | 0.684228 | 7:-62911492 | MsG0780039369.01.T01:CDS |
| AAATTCAAACTACAAACAGT+TGG | 0.617121 | 7:+62911752 | None:intergenic |
| AACAAATGTGGTTTCTGAAT+AGG | 0.395887 | 7:+62910746 | None:intergenic |
| AAGCAGTGCTTCCCAAACCT+TGG | 0.575547 | 7:+62910791 | None:intergenic |
| AATGAAGGAGATGAGGTTGA+AGG | 0.515393 | 7:-62911039 | MsG0780039369.01.T01:CDS |
| AATTATCTTACCACTGAATG+GGG | 0.611534 | 7:+62913728 | None:intergenic |
| ACAATTATCTTACCACTGAA+TGG | 0.436350 | 7:+62913726 | None:intergenic |
| ACATATAGGATCATGCTTGA+TGG | 0.418497 | 7:-62910996 | MsG0780039369.01.T01:CDS |
| ACATTATAAGTATAAACATC+AGG | 0.549214 | 7:+62911218 | None:intergenic |
| ACGATGATATTCAAGTGTCA+AGG | 0.630752 | 7:-62913840 | MsG0780039369.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAATGTGGTTTCTGAAT+AGG | + | Chr7:62913819-62913838 | None:intergenic | 30.0% | |
| AATTATCTTACCACTGAATG+GGG | + | Chr7:62910837-62910856 | None:intergenic | 30.0% | |
| ACAATTATCTTACCACTGAA+TGG | + | Chr7:62910839-62910858 | None:intergenic | 30.0% | |
| AGACATAGAATGATCTGTTA+AGG | + | Chr7:62912038-62912057 | None:intergenic | 30.0% | |
| AGATTAAATTCTTGTTAGGC+TGG | - | Chr7:62911287-62911306 | MsG0780039369.01.T01:CDS | 30.0% | |
| AGCTAAACTTGCATACATAT+AGG | - | Chr7:62913552-62913571 | MsG0780039369.01.T01:intron | 30.0% | |
| ATGAATTGTATGGTAGAAGA+TGG | - | Chr7:62912993-62913012 | MsG0780039369.01.T01:intron | 30.0% | |
| ATGAGAGTTGAAAGAAGAAA+AGG | + | Chr7:62912543-62912562 | None:intergenic | 30.0% | |
| ATTTCTCATTGTGTTCAAGA+TGG | + | Chr7:62912680-62912699 | None:intergenic | 30.0% | |
| CAATTATCTTACCACTGAAT+GGG | + | Chr7:62910838-62910857 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 62910725 | 62913859 | 62910725 | ID=MsG0780039369.01;Name=MsG0780039369.01 |
| Chr7 | mRNA | 62910725 | 62913859 | 62910725 | ID=MsG0780039369.01.T01;Parent=MsG0780039369.01;Name=MsG0780039369.01.T01;_AED=0.47;_eAED=0.49;_QI=0|0|0|1|0|0.5|2|0|439 |
| Chr7 | exon | 62913663 | 62913859 | 62913663 | ID=MsG0780039369.01.T01:exon:16056;Parent=MsG0780039369.01.T01 |
| Chr7 | exon | 62910725 | 62911847 | 62910725 | ID=MsG0780039369.01.T01:exon:16057;Parent=MsG0780039369.01.T01 |
| Chr7 | CDS | 62913663 | 62913859 | 62913663 | ID=MsG0780039369.01.T01:cds;Parent=MsG0780039369.01.T01 |
| Chr7 | CDS | 62910725 | 62911847 | 62910725 | ID=MsG0780039369.01.T01:cds;Parent=MsG0780039369.01.T01 |
| Gene Sequence |
>MsG0780039369.01.T01 ATGATATTCAAGTGTCAAGGAATGGACCTCATTTCGTTGGCACTTGAATACCATCGTTCAAGATGCTCATCGAATGTTGGAGTCCTGCTGCTTAGCAAGTTTGAATTTGCCCCATTCAGTGGTAAGATAATTGTTGCAGTTGGAAGGTACATTCCGGAATGTAATGTAATTGTTGACATACATGTCGTTTATTTAATATTCTATGGTTTTAATGATAGGGTTCAAGATGCTGTTGATTTGTTTTTTAGAATTCCGAGGTTTAGATGCACACCAACTGTTTGTAGTTTGAATTTGTTGCTTTCATTGCTTTGTAGGAAGAGGGAGTGTTTGAAAATGGTTCCGGATATTTTGTTGAAAAGTCGGGATTTGAAGATTCGTCTTGAGGAATCGAGTTTTTGGGTTTTGATTAAGGCGTTGTGTAGGATTAAGAGAGTTGATTATGCTATTAAGATGATGAATTGTATGGTAGAAGATGGTTATGGTTTGGATGATAAGATATGTTCTTTGATAATATCGTCGTTGTTTGAGCAAAATGATTTGACTAGCGTCGAGGCTTTGGTTGTTTGGGGAAATATGAGGAAACTAGGGTTTTGTCCGGGTGTAATGGATTGTACCAATATGATTAGGTTTTTGGTGAAGGGAGGGAAGGGTAAGGATGCTTTGGAGATTTTGAATCAGCTTAAGGAAGATGGAATTAAGCCTGATATTGTTTGTTATACTATTGTTTTGAGTGGAATTGTTAAAGAAGGTGATTATGTGAAGTTAGATGAATTGTTTGATGAAATACTTGTATTAGGACTCGTTCCTGATGTTTATACTTATAATGTGTATATAAATGGTTTGTGTAAGCAGAATAATTTTGATGAGGCACTTAAGATTGTTGTTTCTATGGAAAAATTAGGATGCAAACCAAATGTGGTTACTTATAATACTTTATTAGGTGCATTGTGTATGAATGGAGATTTAGGTAAGGCAAAGAGGGTAATGAAGGAGATGAGGTTGAAGGGTGTTGAGCTAAACTTGCATACATATAGGATCATGCTTGATGGTTTGATTGGTAAAGGTGAGATTGGTGAAGCATGTGTTTTCTTGGAGGAAATGTTGGAGAAGTGTTTTTATCCTAGATCTTCAACATTTGATAGTATCGTACATCAAATGTGCCAAAAGGGTTTGATTAGTGATGCACTTGTGTTGATGAATAAAATTGCTGAAAAAAGTTTTGATCCAGGAGCCAAGGTTTGGGAAGCACTGCTTCTTAACTCAGAATCTAAGGTTGCCTATTCAGAAACCACATTTGTTGGATTGTTGAGGCTAAAATAA |
| Protein sequence |
>MsG0780039369.01.T01 MIFKCQGMDLISLALEYHRSRCSSNVGVLLLSKFEFAPFSGKIIVAVGRYIPECNVIVDIHVVYLIFYGFNDRVQDAVDLFFRIPRFRCTPTVCSLNLLLSLLCRKRECLKMVPDILLKSRDLKIRLEESSFWVLIKALCRIKRVDYAIKMMNCMVEDGYGLDDKICSLIISSLFEQNDLTSVEALVVWGNMRKLGFCPGVMDCTNMIRFLVKGGKGKDALEILNQLKEDGIKPDIVCYTIVLSGIVKEGDYVKLDELFDEILVLGLVPDVYTYNVYINGLCKQNNFDEALKIVVSMEKLGCKPNVVTYNTLLGALCMNGDLGKAKRVMKEMRLKGVELNLHTYRIMLDGLIGKGEIGEACVFLEEMLEKCFYPRSSTFDSIVHQMCQKGLISDALVLMNKIAEKSFDPGAKVWEALLLNSESKVAYSETTFVGLLRLK* |
