AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035575.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035575.01.T01 MTR_6g084470 98.802 167 2 0 1 167 1 167 9.14e-122 340
MsG0680035575.01.T01 MTR_8g104210 64.368 174 48 7 3 167 4 172 8.58e-61 186
MsG0680035575.01.T01 MTR_8g104210 66.049 162 42 6 14 167 7 163 9.51e-59 181
MsG0680035575.01.T01 MTR_6g018430 42.857 175 85 6 1 167 1 168 7.89e-30 107
MsG0680035575.01.T01 MTR_1g110760 44.099 161 71 5 21 167 5 160 5.64e-22 87.0
MsG0680035575.01.T01 MTR_4g087400 37.888 161 66 7 1 153 1 135 9.44e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035575.01.T01 AT3G02555 46.893 177 69 6 1 167 1 162 1.32e-32 114
MsG0680035575.01.T01 AT3G02555 46.893 177 69 6 1 167 1 162 1.32e-32 114
MsG0680035575.01.T01 AT5G16110 46.739 184 75 8 1 167 67 244 2.86e-32 115
MsG0680035575.01.T01 AT1G68490 40.341 176 86 5 5 167 14 183 8.02e-27 100
MsG0680035575.01.T01 AT1G13390 38.462 182 90 9 1 167 2 176 4.72e-20 82.4
MsG0680035575.01.T01 AT1G13390 38.462 182 90 9 1 167 2 176 4.72e-20 82.4

Find 74 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGGAGCAAAGGAAATTT+TGG 0.134528 6:-108653093 None:intergenic
CTCTAGTTAGGTTTATAAAA+AGG 0.147083 6:+108654868 MsG0680035575.01.T01:three_prime_UTR
CTTGTTCTTTCTCTCTAGTT+AGG 0.247722 6:+108654856 MsG0680035575.01.T01:three_prime_UTR
ATTGCTATAGTGAAAGATTT+GGG 0.272791 6:+108654303 MsG0680035575.01.T01:CDS
TCTCTTTGATATTATCAAAT+AGG 0.276395 6:-108655070 None:intergenic
AGACCAAATCGAATAGGTTT+AGG 0.280991 6:-108653638 None:intergenic
GAAAGATTTGGGGAGCAATT+AGG 0.318109 6:+108654314 MsG0680035575.01.T01:CDS
ATATATGTAAATATCAAAAT+TGG 0.318457 6:+108654694 MsG0680035575.01.T01:three_prime_UTR
CAGTCAGAGGTTGAAGATTC+AGG 0.321864 6:+108653823 MsG0680035575.01.T01:CDS
CTCAAAATTTGTGGCCTAAA+GGG 0.326387 6:-108653683 None:intergenic
GTGATCCAAGATGAACAATT+TGG 0.329036 6:+108654392 MsG0680035575.01.T01:CDS
GTGGCCTAAAGGGTCTGATA+TGG 0.337002 6:-108653673 None:intergenic
AGGTTGAAGATTCAGGTGTT+AGG 0.346664 6:+108653830 MsG0680035575.01.T01:CDS
TGCCCTAAACCTATTCGATT+TGG 0.350421 6:+108653635 MsG0680035575.01.T01:CDS
GAAGAGGGTGCCATGGAAAA+TGG 0.350699 6:-108654443 None:intergenic
AATTGCTATAGTGAAAGATT+TGG 0.355756 6:+108654302 MsG0680035575.01.T01:CDS
AGGTGTTAGGGAAGATCTTC+TGG 0.363892 6:+108653843 MsG0680035575.01.T01:CDS
GAGGTCATGTTTATTATTAG+AGG 0.367665 6:+108654811 MsG0680035575.01.T01:three_prime_UTR
TTAAAATACCTCAAAATTTG+TGG 0.380720 6:-108653692 None:intergenic
GACCAAATCGAATAGGTTTA+GGG 0.384037 6:-108653637 None:intergenic
CATTGCCAAATTGTTCATCT+TGG 0.385266 6:-108654397 None:intergenic
CCTCAAAATTTGTGGCCTAA+AGG 0.407341 6:-108653684 None:intergenic
ATAACACATTGAATTAAGAT+TGG 0.414067 6:-108654667 None:intergenic
TTTGTGGATCTCCACCTAGC+AGG 0.415060 6:+108654357 MsG0680035575.01.T01:CDS
CTCCAAATTACAATTACAAA+AGG 0.421159 6:-108654975 None:intergenic
TGTGCTCCAATGAAGAAGTT+TGG 0.423704 6:+108654488 MsG0680035575.01.T01:CDS
GGTTGAAGATTCAGGTGTTA+GGG 0.425014 6:+108653831 MsG0680035575.01.T01:CDS
TTGTAATTTGGAGGAAAATG+AGG 0.440902 6:+108654985 MsG0680035575.01.T01:three_prime_UTR
AGAGATGAGGATGGAATCTC+TGG 0.450122 6:+108653610 MsG0680035575.01.T01:CDS
CAAGCTGCAGTGAGGATTGA+AGG 0.451676 6:+108654518 MsG0680035575.01.T01:CDS
GTGTGACCAAACTTCTTCAT+TGG 0.453010 6:-108654494 None:intergenic
GAGAGTTACCTGAGCTAAGC+TGG 0.458037 6:-108653150 None:intergenic
AATTGTTCATCTTGGATCAC+AGG 0.459752 6:-108654389 None:intergenic
TTTGGCAATGGCAATGGTCA+TGG 0.463708 6:+108654410 MsG0680035575.01.T01:CDS
TTCATCAGACCAAATCGAAT+AGG 0.466136 6:-108653644 None:intergenic
TGTAATTTGGAGGAAAATGA+GGG 0.477912 6:+108654986 MsG0680035575.01.T01:three_prime_UTR
GAAACCATATCAGACCCTTT+AGG 0.483980 6:+108653669 MsG0680035575.01.T01:CDS
GTGTCATGTAAAATTGAGAT+GGG 0.484590 6:+108654745 MsG0680035575.01.T01:three_prime_UTR
TTGTGGATCTCCACCTAGCA+GGG 0.495640 6:+108654358 MsG0680035575.01.T01:CDS
AGGTCATGTTTATTATTAGA+GGG 0.497411 6:+108654812 MsG0680035575.01.T01:three_prime_UTR
TTGATTGTCTCAATAGGGAT+AGG 0.498503 6:+108654543 MsG0680035575.01.T01:CDS
CATCTCTTCACAGCTTCTCA+TGG 0.503294 6:-108653594 None:intergenic
TCTTAATGAAATCCACATGA+AGG 0.528410 6:-108654907 None:intergenic
ATAGAGTTCTAAACAGCAAG+TGG 0.535502 6:+108654622 MsG0680035575.01.T01:three_prime_UTR
GGTGTCATGTAAAATTGAGA+TGG 0.539155 6:+108654744 MsG0680035575.01.T01:three_prime_UTR
ATAGGTGACACTTACAAAGA+AGG 0.553069 6:-108655052 None:intergenic
TCACCATCTTCCTCAGCTAG+AGG 0.554835 6:+108654464 MsG0680035575.01.T01:CDS
TTGGCAATGGCAATGGTCAT+GGG 0.558206 6:+108654411 MsG0680035575.01.T01:CDS
TCTCTGAGCCAGCTTAGCTC+AGG 0.560746 6:+108653142 MsG0680035575.01.T01:five_prime_UTR
GAACAATTTGGCAATGGCAA+TGG 0.561621 6:+108654404 MsG0680035575.01.T01:CDS
GACTCCTCAGAGAGGAGAAG+AGG 0.561825 6:-108653121 None:intergenic
TTAGAATGAAAACCTTCATG+TGG 0.565331 6:+108654895 MsG0680035575.01.T01:three_prime_UTR
CAACCTCTAGCTGAGGAAGA+TGG 0.571591 6:-108654467 None:intergenic
ATTGTTCATCTTGGATCACA+GGG 0.573361 6:-108654388 None:intergenic
AAGCTGTGAAGAGATGAGGA+TGG 0.577435 6:+108653601 MsG0680035575.01.T01:CDS
TCATGTTTATTATTAGAGGG+AGG 0.578993 6:+108654815 MsG0680035575.01.T01:three_prime_UTR
TGTTCCTCTTCTCCTCTCTG+AGG 0.579802 6:+108653117 MsG0680035575.01.T01:five_prime_UTR
AGCTGAGGAAGATGGTGAAG+AGG 0.581033 6:-108654459 None:intergenic
CAAGATGAACAATTTGGCAA+TGG 0.587612 6:+108654398 MsG0680035575.01.T01:CDS
AGATGGTGAAGAGGGTGCCA+TGG 0.590017 6:-108654450 None:intergenic
ACAGGGTTTGAAGCCCTGCT+AGG 0.591659 6:-108654371 None:intergenic
CAAATTACAATTACAAAAGG+AGG 0.597317 6:-108654972 None:intergenic
GGGTTTGAAGCCCTGCTAGG+TGG 0.606726 6:-108654368 None:intergenic
GCTGAGGAAGATGGTGAAGA+GGG 0.607679 6:-108654458 None:intergenic
TCTGGATATCATTCTCCCCA+AGG 0.612920 6:+108653861 MsG0680035575.01.T01:CDS
TAAATATCAAAATTGGTCTG+TGG 0.614604 6:+108654701 MsG0680035575.01.T01:three_prime_UTR
TTGCTATAGTGAAAGATTTG+GGG 0.631998 6:+108654304 MsG0680035575.01.T01:CDS
AATAATCAGCTATCAGTCAG+AGG 0.650098 6:+108653810 MsG0680035575.01.T01:intron
TGGAGCACAACCTCTAGCTG+AGG 0.654183 6:-108654474 None:intergenic
GGCTCAGAGACTCCTCAGAG+AGG 0.664516 6:-108653129 None:intergenic
TGAGAAGCTGTGAAGAGATG+AGG 0.665577 6:+108653597 MsG0680035575.01.T01:CDS
TGTCATGTAAAATTGAGATG+GGG 0.683143 6:+108654746 MsG0680035575.01.T01:three_prime_UTR
GTCACACACAAGCTGCAGTG+AGG 0.704483 6:+108654510 MsG0680035575.01.T01:CDS
TGTGAAAAGATGAATCACTG+TGG 0.839717 6:+108653554 MsG0680035575.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATGTAAATATCAAAAT+TGG + Chr6:108654694-108654713 MsG0680035575.01.T01:three_prime_UTR 10.0%
!!! ATTTATTTTTTTAAGCAAAT+TGG + Chr6:108653369-108653388 MsG0680035575.01.T01:intron 10.0%
!!! AAAACAAATTTTGATCAAAA+GGG + Chr6:108653294-108653313 MsG0680035575.01.T01:intron 15.0%
!!! ATTTTTACAAAAACAATTGA+TGG - Chr6:108653764-108653783 None:intergenic 15.0%
!!! CTTAATTTTTCATGAAAATA+AGG + Chr6:108653902-108653921 MsG0680035575.01.T01:intron 15.0%
!!! TTAATTTGAAGAATAAAAAG+AGG + Chr6:108654792-108654811 MsG0680035575.01.T01:three_prime_UTR 15.0%
!! ATAACACATTGAATTAAGAT+TGG - Chr6:108654670-108654689 None:intergenic 20.0%
!! ATGTTTAATGAACTTAGTTA+AGG + Chr6:108654059-108654078 MsG0680035575.01.T01:intron 20.0%
!! CTGAAAAATCTTGTAATATA+TGG + Chr6:108655016-108655035 MsG0680035575.01.T01:three_prime_UTR 20.0%
!! TCTAGTTAGGTTTATAAAAA+GGG + Chr6:108654869-108654888 MsG0680035575.01.T01:three_prime_UTR 20.0%
!! TCTCTTTGATATTATCAAAT+AGG - Chr6:108655073-108655092 None:intergenic 20.0%
!! TTAAAATACCTCAAAATTTG+TGG - Chr6:108653695-108653714 None:intergenic 20.0%
!!! AACAATGATTTGTTTTGATT+TGG + Chr6:108654267-108654286 MsG0680035575.01.T01:intron 20.0%
!!! ACAAATTTTGAGGTATTTTA+AGG + Chr6:108653694-108653713 MsG0680035575.01.T01:intron 20.0%
!!! ATTTGAGATTTTTTTCATTG+AGG - Chr6:108653244-108653263 None:intergenic 20.0%
!!! CAAAACAAATTTTGATCAAA+AGG + Chr6:108653293-108653312 MsG0680035575.01.T01:intron 20.0%
!!! CAAATTTTGAGGTATTTTAA+GGG + Chr6:108653695-108653714 MsG0680035575.01.T01:intron 20.0%
!!! GTTTCAATTTTGTTTTGATT+TGG + Chr6:108653432-108653451 MsG0680035575.01.T01:intron 20.0%
!!! TGTAATATATGGTTTTTTGT+GGG + Chr6:108655027-108655046 MsG0680035575.01.T01:three_prime_UTR 20.0%
!!! TTGTAATATATGGTTTTTTG+TGG + Chr6:108655026-108655045 MsG0680035575.01.T01:three_prime_UTR 20.0%
! AATTGCTATAGTGAAAGATT+TGG + Chr6:108654302-108654321 MsG0680035575.01.T01:CDS 25.0%
! ACCAAACCAATTATGAAAAA+AGG + Chr6:108653480-108653499 MsG0680035575.01.T01:intron 25.0%
! AGGTCATGTTTATTATTAGA+GGG + Chr6:108654812-108654831 MsG0680035575.01.T01:three_prime_UTR 25.0%
! ATTGCTATAGTGAAAGATTT+GGG + Chr6:108654303-108654322 MsG0680035575.01.T01:CDS 25.0%
! CAAATTACAATTACAAAAGG+AGG - Chr6:108654975-108654994 None:intergenic 25.0%
! CCAAACCAATTATGAAAAAA+GGG + Chr6:108653481-108653500 MsG0680035575.01.T01:intron 25.0%
! CTCCAAATTACAATTACAAA+AGG - Chr6:108654978-108654997 None:intergenic 25.0%
! CTCTAGTTAGGTTTATAAAA+AGG + Chr6:108654868-108654887 MsG0680035575.01.T01:three_prime_UTR 25.0%
! GAAAAATTGTTCATGAGTTA+AGG + Chr6:108654723-108654742 MsG0680035575.01.T01:three_prime_UTR 25.0%
! GAAAATGATGAAATCCAAAA+GGG - Chr6:108653274-108653293 None:intergenic 25.0%
! TGAAAATGATGAAATCCAAA+AGG - Chr6:108653275-108653294 None:intergenic 25.0%
! TTGACATAGAAATTTACCTT+GGG - Chr6:108653880-108653899 None:intergenic 25.0%
! TTTGACATAGAAATTTACCT+TGG - Chr6:108653881-108653900 None:intergenic 25.0%
!! CTCAAATTTTATAACCCTTT+TGG + Chr6:108653257-108653276 MsG0680035575.01.T01:intron 25.0%
!! CTCCTTTTGTAATTGTAATT+TGG + Chr6:108654973-108654992 MsG0680035575.01.T01:three_prime_UTR 25.0%
!! CTTTTGTAATTGTAATTTGG+AGG + Chr6:108654976-108654995 MsG0680035575.01.T01:three_prime_UTR 25.0%
!! TAAATATCAAAATTGGTCTG+TGG + Chr6:108654701-108654720 MsG0680035575.01.T01:three_prime_UTR 25.0%
!! TCAATTCAATTACCATGTTT+TGG + Chr6:108653939-108653958 MsG0680035575.01.T01:intron 25.0%
!! TCTTTTCAATTGAATTTTGC+AGG + Chr6:108653515-108653534 MsG0680035575.01.T01:intron 25.0%
!! TTTTAATCTCTTCAAGTAGT+TGG + Chr6:108653345-108653364 MsG0680035575.01.T01:intron 25.0%
!!! AAACACCCTTTTTTCATAAT+TGG - Chr6:108653489-108653508 None:intergenic 25.0%
!!! GGTTCAATTTTGTTTAGATT+TGG + Chr6:108653390-108653409 MsG0680035575.01.T01:intron 25.0%
CATCAATCATAACCAAAACA+TGG - Chr6:108653954-108653973 None:intergenic 30.0%
CCTTCTGACTGAAATAAAAT+CGG + Chr6:108654116-108654135 MsG0680035575.01.T01:intron 30.0%
GAGGTCATGTTTATTATTAG+AGG + Chr6:108654811-108654830 MsG0680035575.01.T01:three_prime_UTR 30.0%
GTGTCATGTAAAATTGAGAT+GGG + Chr6:108654745-108654764 MsG0680035575.01.T01:three_prime_UTR 30.0%
TCATGTTTATTATTAGAGGG+AGG + Chr6:108654815-108654834 MsG0680035575.01.T01:three_prime_UTR 30.0%
TCTTAATGAAATCCACATGA+AGG - Chr6:108654910-108654929 None:intergenic 30.0%
TGACATAGAAATTTACCTTG+GGG - Chr6:108653879-108653898 None:intergenic 30.0%
TGTAATTTGGAGGAAAATGA+GGG + Chr6:108654986-108655005 MsG0680035575.01.T01:three_prime_UTR 30.0%
TGTCATGTAAAATTGAGATG+GGG + Chr6:108654746-108654765 MsG0680035575.01.T01:three_prime_UTR 30.0%
TTAGAATGAAAACCTTCATG+TGG + Chr6:108654895-108654914 MsG0680035575.01.T01:three_prime_UTR 30.0%
TTGCTATAGTGAAAGATTTG+GGG + Chr6:108654304-108654323 MsG0680035575.01.T01:CDS 30.0%
TTGTAATTTGGAGGAAAATG+AGG + Chr6:108654985-108655004 MsG0680035575.01.T01:three_prime_UTR 30.0%
! AAACAGAACCTTTTTCTCAA+AGG - Chr6:108653178-108653197 None:intergenic 30.0%
! AAATGGAGCAAAGGAAATTT+TGG - Chr6:108653096-108653115 None:intergenic 30.0%
! AACAGAACCTTTTTCTCAAA+GGG - Chr6:108653177-108653196 None:intergenic 30.0%
! AATGGAGCAAAGGAAATTTT+GGG - Chr6:108653095-108653114 None:intergenic 30.0%
! AGATTTTGCATCATACCTTA+AGG + Chr6:108653204-108653223 MsG0680035575.01.T01:intron 30.0%
!! GAATTTTAGTCCATTTTCCA+TGG + Chr6:108654433-108654452 MsG0680035575.01.T01:CDS 30.0%
!!! AAGGTTTTGATTGTCTCAAT+AGG + Chr6:108654537-108654556 MsG0680035575.01.T01:CDS 30.0%
!!! AGGTTTTGATTGTCTCAATA+GGG + Chr6:108654538-108654557 MsG0680035575.01.T01:CDS 30.0%
!!! CCCTTTTTTCATAATTGGTT+TGG - Chr6:108653484-108653503 None:intergenic 30.0%
!!! GTTTTGATTTGGATTTTGCA+GGG + Chr6:108654278-108654297 MsG0680035575.01.T01:intron 30.0%
!!! TGTTTTGATTTGGATTTTGC+AGG + Chr6:108654277-108654296 MsG0680035575.01.T01:intron 30.0%
AATAATCAGCTATCAGTCAG+AGG + Chr6:108653810-108653829 MsG0680035575.01.T01:intron 35.0%
AATTGTTCATCTTGGATCAC+AGG - Chr6:108654392-108654411 None:intergenic 35.0%
AGACCAAATCGAATAGGTTT+AGG - Chr6:108653641-108653660 None:intergenic 35.0%
ATAGGTGACACTTACAAAGA+AGG - Chr6:108655055-108655074 None:intergenic 35.0%
ATTCTCATGGAAAATCGTCA+TGG - Chr6:108654169-108654188 None:intergenic 35.0%
ATTGTTCATCTTGGATCACA+GGG - Chr6:108654391-108654410 None:intergenic 35.0%
CAAGATGAACAATTTGGCAA+TGG + Chr6:108654398-108654417 MsG0680035575.01.T01:CDS 35.0%
CAGTCAGAAGGTAATAAAGT+CGG - Chr6:108654107-108654126 None:intergenic 35.0%
CATTGCCAAATTGTTCATCT+TGG - Chr6:108654400-108654419 None:intergenic 35.0%
CTCAAAATTTGTGGCCTAAA+GGG - Chr6:108653686-108653705 None:intergenic 35.0%
CTTGTTCTTTCTCTCTAGTT+AGG + Chr6:108654856-108654875 MsG0680035575.01.T01:three_prime_UTR 35.0%
GACCAAATCGAATAGGTTTA+GGG - Chr6:108653640-108653659 None:intergenic 35.0%
GCATAAACACAAACACCTTA+AGG - Chr6:108653222-108653241 None:intergenic 35.0%
GTGATCCAAGATGAACAATT+TGG + Chr6:108654392-108654411 MsG0680035575.01.T01:CDS 35.0%
TGTGAAAAGATGAATCACTG+TGG + Chr6:108653554-108653573 MsG0680035575.01.T01:exon 35.0%
TGTTGTTGAATCACTTGATC+TGG + Chr6:108654031-108654050 MsG0680035575.01.T01:intron 35.0%
TTCATCAGACCAAATCGAAT+AGG - Chr6:108653647-108653666 None:intergenic 35.0%
TTGATTGTCTCAATAGGGAT+AGG + Chr6:108654543-108654562 MsG0680035575.01.T01:CDS 35.0%
! AAACAGCAAGTGGAGATTTT+TGG + Chr6:108654632-108654651 MsG0680035575.01.T01:three_prime_UTR 35.0%
! GATTTTCCATGAGAATCGAA+GGG + Chr6:108654173-108654192 MsG0680035575.01.T01:intron 35.0%
! GGTGTCATGTAAAATTGAGA+TGG + Chr6:108654744-108654763 MsG0680035575.01.T01:three_prime_UTR 35.0%
!! AAGGAAATTTTGGGTCTCAA+AGG - Chr6:108653086-108653105 None:intergenic 35.0%
!! ATAGAGTTCTAAACAGCAAG+TGG + Chr6:108654622-108654641 MsG0680035575.01.T01:three_prime_UTR 35.0%
!! CCGATTTTATTTCAGTCAGA+AGG - Chr6:108654119-108654138 None:intergenic 35.0%
AGGTTGAAGATTCAGGTGTT+AGG + Chr6:108653830-108653849 MsG0680035575.01.T01:CDS 40.0%
CCTCAAAATTTGTGGCCTAA+AGG - Chr6:108653687-108653706 None:intergenic 40.0%
GAAACCATATCAGACCCTTT+AGG + Chr6:108653669-108653688 MsG0680035575.01.T01:CDS 40.0%
GAAAGATTTGGGGAGCAATT+AGG + Chr6:108654314-108654333 MsG0680035575.01.T01:CDS 40.0%
GAACAATTTGGCAATGGCAA+TGG + Chr6:108654404-108654423 MsG0680035575.01.T01:CDS 40.0%
GAGGAACAAAAATGGAGCAA+AGG - Chr6:108653105-108653124 None:intergenic 40.0%
GAGGAGAAGAGGAACAAAAA+TGG - Chr6:108653113-108653132 None:intergenic 40.0%
GATCCACAAAAAAATGGTGG+TGG - Chr6:108654347-108654366 None:intergenic 40.0%
GGAGATCCACAAAAAAATGG+TGG - Chr6:108654350-108654369 None:intergenic 40.0%
GGTGGAGATCCACAAAAAAA+TGG - Chr6:108654353-108654372 None:intergenic 40.0%
GGTTGAAGATTCAGGTGTTA+GGG + Chr6:108653831-108653850 MsG0680035575.01.T01:CDS 40.0%
GTGTGACCAAACTTCTTCAT+TGG - Chr6:108654497-108654516 None:intergenic 40.0%
TAGGCTGGAAAAAAGAAGCT+TGG + Chr6:108654198-108654217 MsG0680035575.01.T01:intron 40.0%
TGCCCTAAACCTATTCGATT+TGG + Chr6:108653635-108653654 MsG0680035575.01.T01:CDS 40.0%
TGTGCTCCAATGAAGAAGTT+TGG + Chr6:108654488-108654507 MsG0680035575.01.T01:CDS 40.0%
! ACTCTCTCCCTTTGAGAAAA+AGG + Chr6:108653167-108653186 MsG0680035575.01.T01:intron 40.0%
! CCTTTAGGCCACAAATTTTG+AGG + Chr6:108653684-108653703 MsG0680035575.01.T01:CDS 40.0%
! CGATTTTCCATGAGAATCGA+AGG + Chr6:108654172-108654191 MsG0680035575.01.T01:intron 40.0%
!!! TCACCACCACCATTTTTTTG+TGG + Chr6:108654341-108654360 MsG0680035575.01.T01:CDS 40.0%
AAGCTGTGAAGAGATGAGGA+TGG + Chr6:108653601-108653620 MsG0680035575.01.T01:CDS 45.0%
AGAGATGAGGATGGAATCTC+TGG + Chr6:108653610-108653629 MsG0680035575.01.T01:CDS 45.0%
AGGTGTTAGGGAAGATCTTC+TGG + Chr6:108653843-108653862 MsG0680035575.01.T01:CDS 45.0%
CAGTCAGAGGTTGAAGATTC+AGG + Chr6:108653823-108653842 MsG0680035575.01.T01:CDS 45.0%
CATCTCTTCACAGCTTCTCA+TGG - Chr6:108653597-108653616 None:intergenic 45.0%
CCATGAGAATCGAAGGGTTT+AGG + Chr6:108654179-108654198 MsG0680035575.01.T01:intron 45.0%
CCTAAACCCTTCGATTCTCA+TGG - Chr6:108654182-108654201 None:intergenic 45.0%
TCTGGATATCATTCTCCCCA+AGG + Chr6:108653861-108653880 MsG0680035575.01.T01:CDS 45.0%
TGAGAAGCTGTGAAGAGATG+AGG + Chr6:108653597-108653616 MsG0680035575.01.T01:CDS 45.0%
TTGGCAATGGCAATGGTCAT+GGG + Chr6:108654411-108654430 MsG0680035575.01.T01:CDS 45.0%
TTTGGCAATGGCAATGGTCA+TGG + Chr6:108654410-108654429 MsG0680035575.01.T01:CDS 45.0%
!!! GTGGAGATTTTTGGTGTCAG+AGG + Chr6:108654641-108654660 MsG0680035575.01.T01:three_prime_UTR 45.0%
!!! ATTAAAAAAAATGATTTTTA+AGG - Chr6:108653332-108653351 None:intergenic 5.0%
AGCTGAGGAAGATGGTGAAG+AGG - Chr6:108654462-108654481 None:intergenic 50.0%
CAACCTCTAGCTGAGGAAGA+TGG - Chr6:108654470-108654489 None:intergenic 50.0%
CAAGCTGCAGTGAGGATTGA+AGG + Chr6:108654518-108654537 MsG0680035575.01.T01:CDS 50.0%
GAGAATCGAAGGGTTTAGGC+TGG + Chr6:108654183-108654202 MsG0680035575.01.T01:intron 50.0%
GAGAGTTACCTGAGCTAAGC+TGG - Chr6:108653153-108653172 None:intergenic 50.0%
GCTGAGGAAGATGGTGAAGA+GGG - Chr6:108654461-108654480 None:intergenic 50.0%
GTGGCCTAAAGGGTCTGATA+TGG - Chr6:108653676-108653695 None:intergenic 50.0%
TCACCATCTTCCTCAGCTAG+AGG + Chr6:108654464-108654483 MsG0680035575.01.T01:CDS 50.0%
TGTTCCTCTTCTCCTCTCTG+AGG + Chr6:108653117-108653136 MsG0680035575.01.T01:five_prime_UTR 50.0%
TTGTGGATCTCCACCTAGCA+GGG + Chr6:108654358-108654377 MsG0680035575.01.T01:CDS 50.0%
TTTGTGGATCTCCACCTAGC+AGG + Chr6:108654357-108654376 MsG0680035575.01.T01:CDS 50.0%
!! GAAGAGGGTGCCATGGAAAA+TGG - Chr6:108654446-108654465 None:intergenic 50.0%
GACTCCTCAGAGAGGAGAAG+AGG - Chr6:108653124-108653143 None:intergenic 55.0%
GTCACACACAAGCTGCAGTG+AGG + Chr6:108654510-108654529 MsG0680035575.01.T01:CDS 55.0%
TCTCTGAGCCAGCTTAGCTC+AGG + Chr6:108653142-108653161 MsG0680035575.01.T01:five_prime_UTR 55.0%
TGGAGCACAACCTCTAGCTG+AGG - Chr6:108654477-108654496 None:intergenic 55.0%
! ACAGGGTTTGAAGCCCTGCT+AGG - Chr6:108654374-108654393 None:intergenic 55.0%
!! AGATGGTGAAGAGGGTGCCA+TGG - Chr6:108654453-108654472 None:intergenic 55.0%
GGCTCAGAGACTCCTCAGAG+AGG - Chr6:108653132-108653151 None:intergenic 60.0%
! GGGTTTGAAGCCCTGCTAGG+TGG - Chr6:108654371-108654390 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 108653066 108655108 108653066 ID=MsG0680035575.01;Name=MsG0680035575.01
Chr6 mRNA 108653066 108655108 108653066 ID=MsG0680035575.01.T01;Parent=MsG0680035575.01;Name=MsG0680035575.01.T01;_AED=0.24;_eAED=0.24;_QI=124|1|1|1|0.66|0.5|4|513|167
Chr6 exon 108653066 108653163 108653066 ID=MsG0680035575.01.T01:exon:14438;Parent=MsG0680035575.01.T01
Chr6 exon 108653537 108653705 108653537 ID=MsG0680035575.01.T01:exon:14439;Parent=MsG0680035575.01.T01
Chr6 exon 108653819 108653882 108653819 ID=MsG0680035575.01.T01:exon:14440;Parent=MsG0680035575.01.T01
Chr6 exon 108654299 108655108 108654299 ID=MsG0680035575.01.T01:exon:14441;Parent=MsG0680035575.01.T01
Chr6 five_prime_UTR 108653066 108653163 108653066 ID=MsG0680035575.01.T01:five_prime_utr;Parent=MsG0680035575.01.T01
Chr6 five_prime_UTR 108653537 108653562 108653537 ID=MsG0680035575.01.T01:five_prime_utr;Parent=MsG0680035575.01.T01
Chr6 CDS 108653563 108653705 108653563 ID=MsG0680035575.01.T01:cds;Parent=MsG0680035575.01.T01
Chr6 CDS 108653819 108653882 108653819 ID=MsG0680035575.01.T01:cds;Parent=MsG0680035575.01.T01
Chr6 CDS 108654299 108654595 108654299 ID=MsG0680035575.01.T01:cds;Parent=MsG0680035575.01.T01
Chr6 three_prime_UTR 108654596 108655108 108654596 ID=MsG0680035575.01.T01:three_prime_utr;Parent=MsG0680035575.01.T01
Gene Sequence

>MsG0680035575.01.T01

ATGAATCACTGTGGCTATCAACAGAAGAACGCCATGAGAAGCTGTGAAGAGATGAGGATGGAATCTCTGGTTTGCCCTAAACCTATTCGATTTGGTCTGATGAACAGAAACCATATCAGACCCTTTAGGCCACAAATTTTGAGCTATCAGTCAGAGGTTGAAGATTCAGGTGTTAGGGAAGATCTTCTGGATATCATTCTCCCCAAGGGAAATTGCTATAGTGAAAGATTTGGGGAGCAATTAGGATCATCACCACCACCATTTTTTTGTGGATCTCCACCTAGCAGGGCTTCAAACCCTGTGATCCAAGATGAACAATTTGGCAATGGCAATGGTCATGGGAATTTTAGTCCATTTTCCATGGCACCCTCTTCACCATCTTCCTCAGCTAGAGGTTGTGCTCCAATGAAGAAGTTTGGTCACACACAAGCTGCAGTGAGGATTGAAGGTTTTGATTGTCTCAATAGGGATAGGAGCAACTGCAGCATTTCAGCTGTTGCATAA
Protein sequence

>MsG0680035575.01.T01

MNHCGYQQKNAMRSCEEMRMESLVCPKPIRFGLMNRNHIRPFRPQILSYQSEVEDSGVREDLLDIILPKGNCYSERFGEQLGSSPPPFFCGSPPSRASNPVIQDEQFGNGNGHGNFSPFSMAPSSPSSSARGCAPMKKFGHTQAAVRIEGFDCLNRDRSNCSISAVA*