AlfalfaGEDB | MsG0480021237.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021237.01

KEGG: -
GO: -
PFAM: PF10950

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021237.01.T01	AES89076.1	85.417	48	7	0	1	48	1	48	4.80E-20	85.9

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021237.01.T01	G7JG36	85.417	48	7	0	1	48	1	48	2.29e-20	85.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049059.01	MsG0480021237.01	0.833178	6.192007e-56	7.075665e-53
MsG0180003409.01	MsG0480021237.01	0.825460	4.631398e-54	4.223823e-51
MsG0180003731.01	MsG0480021237.01	0.827486	1.523435e-54	1.472835e-51
MsG0180006069.01	MsG0480021237.01	0.816124	6.502082e-52	4.567816e-49
MsG0480019038.01	MsG0480021237.01	0.800253	1.571668e-48	7.283868e-46
MsG0480021237.01	MsG0680030690.01	0.801044	1.084119e-48	5.126524e-46
MsG0480021237.01	MsG0680033623.01	0.818435	1.962744e-52	1.469239e-49
MsG0480021237.01	MsG0680035575.01	0.802066	6.691506e-49	3.246898e-46
MsG0480021237.01	MsG0680035777.01	0.843474	1.370130e-58	2.146118e-55
MsG0480021237.01	MsG0880041883.01	0.840876	6.676840e-58	9.641085e-55
MsG0480021237.01	MsG0880047515.01	0.800804	1.213863e-48	5.704649e-46
MsG0380014947.01	MsG0480021237.01	0.806431	8.253365e-50	4.478874e-47
MsG0380016346.01	MsG0480021237.01	0.803973	2.699397e-49	1.375327e-46
MsG0380016348.01	MsG0480021237.01	0.851607	7.950100e-61	1.617806e-57
MsG0380016573.01	MsG0480021237.01	0.802244	6.151605e-49	2.998495e-46
MsG0380017135.01	MsG0480021237.01	-0.813972	1.953004e-51	1.293957e-48
MsG0380017915.01	MsG0480021237.01	0.832995	6.873650e-56	7.812235e-53
MsG0480018160.01	MsG0480021237.01	0.824039	1.001823e-53	8.772872e-51
MsG0480018333.01	MsG0480021237.01	0.846403	2.220295e-59	3.817387e-56
MsG0480018355.01	MsG0480021237.01	0.829151	6.044439e-55	6.134215e-52
MsG0280007052.01	MsG0480021237.01	0.803641	3.163702e-49	1.598150e-46
MsG0280007071.01	MsG0480021237.01	0.812204	4.767919e-51	3.012528e-48
MsG0280010196.01	MsG0480021237.01	0.840244	9.770269e-58	1.383436e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021237.01.T01	MTR_4g069770	85.417	48	7	1	48	1	48	5.82e-24	85.9
MsG0480021237.01.T01	MTR_5g095980	70.588	51	15	1	51	1	51	1.61e-15	64.7
MsG0480021237.01.T01	MTR_3g007880	64.706	51	18	1	51	1	51	8.40e-13	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATATCTTATATGTTATA+TGG	0.245081	4:+55641439	MsG0480021237.01.T01:three_prime_UTR
GAAAAGACTTGGGAGATTAT+TGG	0.261061	4:+55641196	MsG0480021237.01.T01:CDS
AATTAGATTTGTTAGTTTGA+TGG	0.285742	4:-55640944	None:intergenic
AAAATACATTATAGATAAAA+AGG	0.331556	4:-55641540	None:intergenic
AACTCTGAACAAGAGCTTCA+TGG	0.354626	4:+55641387	MsG0480021237.01.T01:three_prime_UTR
TAGATACATCAATCAAATAT+TGG	0.395866	4:-55641617	None:intergenic
AACAAGTTCTTTGATTGCTC+AGG	0.409554	4:-55641246	None:intergenic
CTCTTATGCAAGAAAAGACT+TGG	0.442343	4:+55641185	MsG0480021237.01.T01:CDS
GAGTATGAGAGAAGCAAAGA+TGG	0.451907	4:-55641001	None:intergenic
GATTTGTTAGTTTGATGGCT+AGG	0.457950	4:-55640939	None:intergenic
GTGTGATATAATATGACATT+AGG	0.480243	4:-55641317	None:intergenic
TTGATTGCTCAGGCATAGGT+TGG	0.490736	4:-55641236	None:intergenic
TTCTTTGATTGCTCAGGCAT+AGG	0.516799	4:-55641240	None:intergenic
TTTGATTGATGTATCTAGTG+TGG	0.527087	4:+55641623	MsG0480021237.01.T01:three_prime_UTR
AGAACTTGTTCAAAATCAAG+AGG	0.539159	4:+55641259	MsG0480021237.01.T01:CDS
TCTTATGCAAGAAAAGACTT+GGG	0.611202	4:+55641186	MsG0480021237.01.T01:CDS
TAAAAGTCCACATCTTAGCA+AGG	0.615141	4:+55640966	MsG0480021237.01.T01:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATACATTATAGATAAAA+AGG	-	Chr4:55641543-55641562	None:intergenic	10.0%
!!	TTAATATCTTATATGTTATA+TGG	+	Chr4:55641439-55641458	MsG0480021237.01.T01:three_prime_UTR	10.0%
!!!	TTATCTATAATGTATTTTTA+TGG	+	Chr4:55641545-55641564	MsG0480021237.01.T01:three_prime_UTR	10.0%
!!!	AATTTTCTTTGTACTAAAAT+AGG	+	Chr4:55641152-55641171	MsG0480021237.01.T01:intron	15.0%
!!!	TATTTAAGGTTTATTTTGTA+GGG	+	Chr4:55641474-55641493	MsG0480021237.01.T01:three_prime_UTR	15.0%
!!	GGAAAAAAGATTAGTATTTA+AGG	+	Chr4:55641460-55641479	MsG0480021237.01.T01:three_prime_UTR	20.0%
!!	TAGATACATCAATCAAATAT+TGG	-	Chr4:55641620-55641639	None:intergenic	20.0%
!!	TTAGTACAAAGAAAATTGAA+AGG	-	Chr4:55641149-55641168	None:intergenic	20.0%
!!!	AATTAGATTTGTTAGTTTGA+TGG	-	Chr4:55640947-55640966	None:intergenic	20.0%
!!!	GTATTTAAGGTTTATTTTGT+AGG	+	Chr4:55641473-55641492	MsG0480021237.01.T01:three_prime_UTR	20.0%
!	GTGTGATATAATATGACATT+AGG	-	Chr4:55641320-55641339	None:intergenic	25.0%
!!!	TTTTTTGCTGCATCTATTTT+TGG	+	Chr4:55641577-55641596	MsG0480021237.01.T01:three_prime_UTR	25.0%
	ATGGAACATAAAAACATGGT+TGG	+	Chr4:55641406-55641425	MsG0480021237.01.T01:three_prime_UTR	30.0%
	CTTCATGGAACATAAAAACA+TGG	+	Chr4:55641402-55641421	MsG0480021237.01.T01:three_prime_UTR	30.0%
	TCTTATGCAAGAAAAGACTT+GGG	+	Chr4:55641186-55641205	MsG0480021237.01.T01:CDS	30.0%
	TTTGATTGATGTATCTAGTG+TGG	+	Chr4:55641623-55641642	MsG0480021237.01.T01:three_prime_UTR	30.0%
!	AGAACTTGTTCAAAATCAAG+AGG	+	Chr4:55641259-55641278	MsG0480021237.01.T01:CDS	30.0%
!!!	ATTTTTTCCTTGCTAAGATG+TGG	-	Chr4:55640976-55640995	None:intergenic	30.0%
	AACAAGTTCTTTGATTGCTC+AGG	-	Chr4:55641249-55641268	None:intergenic	35.0%
	CTCTTATGCAAGAAAAGACT+TGG	+	Chr4:55641185-55641204	MsG0480021237.01.T01:CDS	35.0%
	GAAAAGACTTGGGAGATTAT+TGG	+	Chr4:55641196-55641215	MsG0480021237.01.T01:CDS	35.0%
	TAAAAGTCCACATCTTAGCA+AGG	+	Chr4:55640966-55640985	MsG0480021237.01.T01:five_prime_UTR	35.0%
!	AGTCTTTTCTTGCATAAGAG+AGG	-	Chr4:55641185-55641204	None:intergenic	35.0%
!	CATACTCTTTTCTCTTCTTC+TGG	+	Chr4:55641017-55641036	MsG0480021237.01.T01:CDS	35.0%
!	GAGTTTTTCACAAAGACATG+TGG	-	Chr4:55641372-55641391	None:intergenic	35.0%
!!	AGATTTGTTAGTTTGATGGC+TGG	-	Chr4:55640943-55640962	None:intergenic	35.0%
!!	GATTTGTTAGTTTGATGGCT+GGG	-	Chr4:55640942-55640961	None:intergenic	35.0%
	AACTCTGAACAAGAGCTTCA+TGG	+	Chr4:55641387-55641406	MsG0480021237.01.T01:three_prime_UTR	40.0%
	GAGTATGAGAGAAGCAAAGA+TGG	-	Chr4:55641004-55641023	None:intergenic	40.0%
	TTCTTTGATTGCTCAGGCAT+AGG	-	Chr4:55641243-55641262	None:intergenic	40.0%
!	TGTGGTTTTCTTCTGCTTCT+TGG	-	Chr4:55641354-55641373	None:intergenic	40.0%
	TTGATTGCTCAGGCATAGGT+TGG	-	Chr4:55641239-55641258	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	55640942	55641684	55640942	ID=MsG0480021237.01;Name=MsG0480021237.01
Chr4	mRNA	55640942	55641684	55640942	ID=MsG0480021237.01.T01;Parent=MsG0480021237.01;Name=MsG0480021237.01.T01;_AED=0.14;_eAED=0.14;_QI=52\|1\|1\|1\|0\|0\|2\|370\|61
Chr4	exon	55640942	55641038	55640942	ID=MsG0480021237.01.T01:exon:6789;Parent=MsG0480021237.01.T01
Chr4	exon	55641174	55641684	55641174	ID=MsG0480021237.01.T01:exon:6790;Parent=MsG0480021237.01.T01
Chr4	five_prime_UTR	55640942	55640993	55640942	ID=MsG0480021237.01.T01:five_prime_utr;Parent=MsG0480021237.01.T01
Chr4	CDS	55640994	55641038	55640994	ID=MsG0480021237.01.T01:cds;Parent=MsG0480021237.01.T01
Chr4	CDS	55641174	55641314	55641174	ID=MsG0480021237.01.T01:cds;Parent=MsG0480021237.01.T01
Chr4	three_prime_UTR	55641315	55641684	55641315	ID=MsG0480021237.01.T01:three_prime_utr;Parent=MsG0480021237.01.T01

Gene Sequence	>MsG0480021237.01.T01 ATGAAGTCCATCTTTGCTTCTCTCATACTCTTTTCTCTTCTTCTGGTTGCTGACCTCTCTTATGCAAGAAAAGACTTGGGAGATTATTGGAAGAATATGATGAATGACCAACCTATGCCTGAGCAATCAAAGAACTTGTTCAAAATCAAGAGGTTTCAGATGACTTTATTAGAGACTTTGATGTAA
Protein sequence	>MsG0480021237.01.T01 MKSIFASLILFSLLLVADLSYARKDLGDYWKNMMNDQPMPEQSKNLFKIKRFQMTLLETLM*