Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035788.01.T01 | XP_003620790.1 | 91.011 | 89 | 8 | 0 | 1 | 89 | 1 | 89 | 1.27E-48 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035788.01.T01 | G7KI13 | 91.011 | 89 | 8 | 0 | 1 | 89 | 1 | 89 | 6.05e-49 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001442.01 | MsG0680035788.01 | -0.802939 | 4.421516e-49 | 2.193650e-46 |
| MsG0380011839.01 | MsG0680035788.01 | -0.807450 | 5.023975e-50 | 2.799258e-47 |
| MsG0380013373.01 | MsG0680035788.01 | -0.807940 | 3.953398e-50 | 2.230938e-47 |
| MsG0580026024.01 | MsG0680035788.01 | -0.803830 | 2.891066e-49 | 1.467551e-46 |
| MsG0580026026.01 | MsG0680035788.01 | -0.808171 | 3.530526e-50 | 2.004679e-47 |
| MsG0380017253.01 | MsG0680035788.01 | 0.823030 | 1.724879e-53 | 1.468246e-50 |
| MsG0380017780.01 | MsG0680035788.01 | 0.802404 | 5.702529e-49 | 2.791031e-46 |
| MsG0680035788.01 | MsG0880046135.01 | 0.814691 | 1.354225e-51 | 9.148892e-49 |
| MsG0680035788.01 | MsG0880046573.01 | -0.823799 | 1.139940e-53 | 9.916802e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035788.01.T01 | MTR_6g090490 | 91.011 | 89 | 8 | 0 | 1 | 89 | 1 | 89 | 1.53e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTGATCTTCGAAATT+TGG | 0.115608 | 6:+112276530 | None:intergenic |
| TGGAGCTGCTTTGGATTCTA+AGG | 0.294697 | 6:-112276555 | MsG0680035788.01.T01:CDS |
| TGCAGAACATGGAGCTGCTT+TGG | 0.354322 | 6:-112276564 | MsG0680035788.01.T01:CDS |
| CCTCCGTGACCTCCTTTCTT+CGG | 0.356322 | 6:+112276626 | None:intergenic |
| ACCTCCTTTCTTCGGTGAAC+CGG | 0.365180 | 6:+112276634 | None:intergenic |
| ACGAGATTCCGATGCGGAGG+AGG | 0.466592 | 6:+112276685 | None:intergenic |
| CTTCAGATCGGTGCAGAACA+TGG | 0.498685 | 6:-112276575 | MsG0680035788.01.T01:CDS |
| AAAGATCGCAAATCATCCTC+TGG | 0.510278 | 6:-112276662 | MsG0680035788.01.T01:CDS |
| ACACAACCACCGCAATCAAA+CGG | 0.536314 | 6:+112276466 | None:intergenic |
| AAATCATCCTCTGGAATGAC+CGG | 0.538942 | 6:-112276653 | MsG0680035788.01.T01:CDS |
| CAATCAAACGGTAACGGAAG+CGG | 0.553668 | 6:+112276478 | None:intergenic |
| CGGAAAATCCTCCTCCGCAT+CGG | 0.554876 | 6:-112276693 | MsG0680035788.01.T01:CDS |
| GTCACGGAGGGAAGTTCACA+TGG | 0.573877 | 6:-112276613 | MsG0680035788.01.T01:CDS |
| AAAAGCCACAAATCCACCAC+CGG | 0.574764 | 6:-112276713 | MsG0680035788.01.T01:CDS |
| GGAGGGAAGTTCACATGGAT+CGG | 0.576604 | 6:-112276608 | MsG0680035788.01.T01:CDS |
| TGTTCTGCACCGATCTGAAG+AGG | 0.576691 | 6:+112276578 | None:intergenic |
| ATGACCGGTTCACCGAAGAA+AGG | 0.587391 | 6:-112276638 | MsG0680035788.01.T01:CDS |
| AGCGGTGACAGTGTCAGTGT+CGG | 0.604133 | 6:+112276496 | None:intergenic |
| GGATCTGATCCTCTTCAGAT+CGG | 0.604184 | 6:-112276587 | MsG0680035788.01.T01:CDS |
| CGAAGAAAGGAGGTCACGGA+GGG | 0.619061 | 6:-112276625 | MsG0680035788.01.T01:CDS |
| GGTGACAGTGTCAGTGTCGG+CGG | 0.620679 | 6:+112276499 | None:intergenic |
| ACTTCAACAATGAAATCCAC+CGG | 0.621434 | 6:-112276737 | None:intergenic |
| CGGTGAACCGGTCATTCCAG+AGG | 0.635019 | 6:+112276646 | None:intergenic |
| TCACCGAAGAAAGGAGGTCA+CGG | 0.636627 | 6:-112276629 | MsG0680035788.01.T01:CDS |
| TTTACGAGATTCCGATGCGG+AGG | 0.641309 | 6:+112276682 | None:intergenic |
| ACCGGTTCACCGAAGAAAGG+AGG | 0.641392 | 6:-112276635 | MsG0680035788.01.T01:CDS |
| CCACCGCAATCAAACGGTAA+CGG | 0.649561 | 6:+112276472 | None:intergenic |
| CCGAAGAAAGGAGGTCACGG+AGG | 0.673021 | 6:-112276626 | MsG0680035788.01.T01:CDS |
| ATCTTTACGAGATTCCGATG+CGG | 0.748893 | 6:+112276679 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTTCTTGATCTTCGAAATT+TGG | + | Chr6:112276682-112276701 | None:intergenic | 30.0% | |
| AAAGATCGCAAATCATCCTC+TGG | - | Chr6:112276547-112276566 | MsG0680035788.01.T01:CDS | 40.0% | |
| ATCTTTACGAGATTCCGATG+CGG | + | Chr6:112276533-112276552 | None:intergenic | 40.0% | |
| !! | AAATCATCCTCTGGAATGAC+CGG | - | Chr6:112276556-112276575 | MsG0680035788.01.T01:CDS | 40.0% |
| AAAAGCCACAAATCCACCAC+CGG | - | Chr6:112276496-112276515 | MsG0680035788.01.T01:CDS | 45.0% | |
| GGATCTGATCCTCTTCAGAT+CGG | - | Chr6:112276622-112276641 | MsG0680035788.01.T01:CDS | 45.0% | |
| ! | TGGAGCTGCTTTGGATTCTA+AGG | - | Chr6:112276654-112276673 | MsG0680035788.01.T01:CDS | 45.0% |
| !! | GGTGGATTTGTGGCTTTTTC+CGG | + | Chr6:112276494-112276513 | None:intergenic | 45.0% |
| !!! | ATTTTCCGGTGGTGGATTTG+TGG | + | Chr6:112276504-112276523 | None:intergenic | 45.0% |
| ACCTCCTTTCTTCGGTGAAC+CGG | + | Chr6:112276578-112276597 | None:intergenic | 50.0% | |
| CTTCAGATCGGTGCAGAACA+TGG | - | Chr6:112276634-112276653 | MsG0680035788.01.T01:CDS | 50.0% | |
| GGAGGGAAGTTCACATGGAT+CGG | - | Chr6:112276601-112276620 | MsG0680035788.01.T01:CDS | 50.0% | |
| TCACCGAAGAAAGGAGGTCA+CGG | - | Chr6:112276580-112276599 | MsG0680035788.01.T01:CDS | 50.0% | |
| TGCAGAACATGGAGCTGCTT+TGG | - | Chr6:112276645-112276664 | MsG0680035788.01.T01:CDS | 50.0% | |
| TTTACGAGATTCCGATGCGG+AGG | + | Chr6:112276530-112276549 | None:intergenic | 50.0% | |
| ! | ATGACCGGTTCACCGAAGAA+AGG | - | Chr6:112276571-112276590 | MsG0680035788.01.T01:CDS | 50.0% |
| ! | TGTTCTGCACCGATCTGAAG+AGG | + | Chr6:112276634-112276653 | None:intergenic | 50.0% |
| !! | GGATTTGTGGCTTTTTCCGG+TGG | + | Chr6:112276491-112276510 | None:intergenic | 50.0% |
| AGCGGTGACAGTGTCAGTGT+CGG | + | Chr6:112276716-112276735 | None:intergenic | 55.0% | |
| CCTCCGTGACCTCCTTTCTT+CGG | + | Chr6:112276586-112276605 | None:intergenic | 55.0% | |
| CGAAGAAAGGAGGTCACGGA+GGG | - | Chr6:112276584-112276603 | MsG0680035788.01.T01:CDS | 55.0% | |
| CGGAAAATCCTCCTCCGCAT+CGG | - | Chr6:112276516-112276535 | MsG0680035788.01.T01:CDS | 55.0% | |
| GTCACGGAGGGAAGTTCACA+TGG | - | Chr6:112276596-112276615 | MsG0680035788.01.T01:CDS | 55.0% | |
| ! | ACCGGTTCACCGAAGAAAGG+AGG | - | Chr6:112276574-112276593 | MsG0680035788.01.T01:CDS | 55.0% |
| ! | CGATGCGGAGGAGGATTTTC+CGG | + | Chr6:112276518-112276537 | None:intergenic | 55.0% |
| ACGAGATTCCGATGCGGAGG+AGG | + | Chr6:112276527-112276546 | None:intergenic | 60.0% | |
| CCGAAGAAAGGAGGTCACGG+AGG | - | Chr6:112276583-112276602 | MsG0680035788.01.T01:CDS | 60.0% | |
| CGGTGAACCGGTCATTCCAG+AGG | + | Chr6:112276566-112276585 | None:intergenic | 60.0% | |
| ! | GGAGGAGGATTTTCCGGTGG+TGG | + | Chr6:112276512-112276531 | None:intergenic | 60.0% |
| ! | GGTGACAGTGTCAGTGTCGG+CGG | + | Chr6:112276713-112276732 | None:intergenic | 60.0% |
| ! | TGCGGAGGAGGATTTTCCGG+TGG | + | Chr6:112276515-112276534 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 112276481 | 112276750 | 112276481 | ID=MsG0680035788.01;Name=MsG0680035788.01 |
| Chr6 | mRNA | 112276481 | 112276750 | 112276481 | ID=MsG0680035788.01.T01;Parent=MsG0680035788.01;Name=MsG0680035788.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr6 | exon | 112276481 | 112276750 | 112276481 | ID=MsG0680035788.01.T01:exon:15118;Parent=MsG0680035788.01.T01 |
| Chr6 | CDS | 112276481 | 112276750 | 112276481 | ID=MsG0680035788.01.T01:cds;Parent=MsG0680035788.01.T01 |
| Gene Sequence |
| Protein sequence |
