Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017253.01.T01 | XP_003603107.1 | 94.964 | 278 | 12 | 1 | 1 | 276 | 1 | 278 | 0 | 545 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017253.01.T01 | Q9M0G2 | 45.833 | 288 | 100 | 10 | 43 | 275 | 69 | 355 | 7.35E-68 | 217 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017253.01.T01 | G7J714 | 94.964 | 278 | 12 | 1 | 1 | 276 | 1 | 278 | 0.0 | 545 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000489.01 | MsG0380017253.01 | -0.808606 | 2.852110e-50 | 1.637855e-47 |
| MsG0180000646.01 | MsG0380017253.01 | -0.812459 | 4.194313e-51 | 2.668462e-48 |
| MsG0180000647.01 | MsG0380017253.01 | -0.814094 | 1.835145e-51 | 1.219903e-48 |
| MsG0180002891.01 | MsG0380017253.01 | -0.809246 | 2.080878e-50 | 1.215535e-47 |
| MsG0180004106.01 | MsG0380017253.01 | -0.801522 | 8.653315e-49 | 4.141302e-46 |
| MsG0180004246.01 | MsG0380017253.01 | -0.817605 | 3.023667e-52 | 2.212057e-49 |
| MsG0180005803.01 | MsG0380017253.01 | -0.824752 | 6.807858e-54 | 6.085637e-51 |
| MsG0180005807.01 | MsG0380017253.01 | -0.806970 | 6.352097e-50 | 3.495200e-47 |
| MsG0280010611.01 | MsG0380017253.01 | -0.826229 | 3.041363e-54 | 2.835412e-51 |
| MsG0280010675.01 | MsG0380017253.01 | -0.802700 | 4.953801e-49 | 2.442933e-46 |
| MsG0280011110.01 | MsG0380017253.01 | 0.838861 | 2.235589e-57 | 3.033217e-54 |
| MsG0380013687.01 | MsG0380017253.01 | -0.812700 | 3.714662e-51 | 2.378256e-48 |
| MsG0380014530.01 | MsG0380017253.01 | -0.810886 | 9.220776e-51 | 5.625421e-48 |
| MsG0380015486.01 | MsG0380017253.01 | -0.822624 | 2.143898e-53 | 1.804161e-50 |
| MsG0380017253.01 | MsG0380017780.01 | 0.809119 | 2.214947e-50 | 1.289458e-47 |
| MsG0380017253.01 | MsG0480018232.01 | -0.859844 | 3.122347e-63 | 8.362384e-60 |
| MsG0380017253.01 | MsG0480021060.01 | -0.833316 | 5.720855e-56 | 6.564573e-53 |
| MsG0380017253.01 | MsG0480022617.01 | -0.804804 | 1.811544e-49 | 9.429583e-47 |
| MsG0380017253.01 | MsG0480023998.01 | -0.822368 | 2.458970e-53 | 2.054236e-50 |
| MsG0380017253.01 | MsG0580024404.01 | -0.808055 | 3.737492e-50 | 2.115480e-47 |
| MsG0380017253.01 | MsG0580024820.01 | -0.896190 | 4.677893e-76 | 5.006313e-72 |
| MsG0380017253.01 | MsG0580025845.01 | -0.805048 | 1.611336e-49 | 8.439762e-47 |
| MsG0380017253.01 | MsG0580026050.01 | -0.814006 | 1.919566e-51 | 1.272998e-48 |
| MsG0380017253.01 | MsG0580026271.01 | -0.803206 | 3.894113e-49 | 1.945228e-46 |
| MsG0380017253.01 | MsG0580028093.01 | -0.803461 | 3.447112e-49 | 1.733288e-46 |
| MsG0380017253.01 | MsG0580028719.01 | -0.804463 | 2.134677e-49 | 1.101324e-46 |
| MsG0380017253.01 | MsG0580029276.01 | -0.802835 | 4.646536e-49 | 2.299384e-46 |
| MsG0380017253.01 | MsG0680030680.01 | -0.814027 | 1.899037e-51 | 1.260111e-48 |
| MsG0380017253.01 | MsG0680031998.01 | -0.802354 | 5.837294e-49 | 2.853429e-46 |
| MsG0380017253.01 | MsG0680034402.01 | -0.800324 | 1.520013e-48 | 7.057532e-46 |
| MsG0380017253.01 | MsG0680035788.01 | 0.823030 | 1.724879e-53 | 1.468246e-50 |
| MsG0380017253.01 | MsG0780036509.01 | -0.805400 | 1.359387e-49 | 7.185007e-47 |
| MsG0380017253.01 | MsG0780036517.01 | -0.807756 | 4.325815e-50 | 2.429452e-47 |
| MsG0380017253.01 | MsG0780036584.01 | -0.812424 | 4.270250e-51 | 2.714226e-48 |
| MsG0380017253.01 | MsG0780038334.01 | -0.831431 | 1.676219e-55 | 1.818534e-52 |
| MsG0380017253.01 | MsG0780039179.01 | -0.802745 | 4.849654e-49 | 2.394295e-46 |
| MsG0380017253.01 | MsG0780039186.01 | -0.804013 | 2.647834e-49 | 1.350447e-46 |
| MsG0380017253.01 | MsG0780039256.01 | -0.814497 | 1.495129e-51 | 1.004864e-48 |
| MsG0380017253.01 | MsG0780039338.01 | -0.801948 | 7.077321e-49 | 3.423684e-46 |
| MsG0380017253.01 | MsG0780039339.01 | -0.809642 | 1.710338e-50 | 1.009738e-47 |
| MsG0380017253.01 | MsG0780040278.01 | -0.819517 | 1.113949e-52 | 8.591327e-50 |
| MsG0380017253.01 | MsG0780040895.01 | -0.825019 | 5.889872e-54 | 5.305189e-51 |
| MsG0380017253.01 | MsG0780041074.01 | -0.802193 | 6.299667e-49 | 3.066626e-46 |
| MsG0380017253.01 | MsG0780041088.01 | -0.824632 | 7.266024e-54 | 6.472805e-51 |
| MsG0380017253.01 | MsG0880042876.01 | -0.804634 | 1.965886e-49 | 1.018726e-46 |
| MsG0380017253.01 | MsG0880042988.01 | -0.824174 | 9.311753e-54 | 8.186218e-51 |
| MsG0380017253.01 | MsG0880044043.01 | -0.807445 | 5.036949e-50 | 2.806020e-47 |
| MsG0380017253.01 | MsG0880044410.01 | -0.802788 | 4.750788e-49 | 2.348123e-46 |
| MsG0380017253.01 | MsG0880044538.01 | -0.808571 | 2.900869e-50 | 1.664391e-47 |
| MsG0380017253.01 | MsG0880045668.01 | -0.838320 | 3.085024e-57 | 4.116713e-54 |
| MsG0380017253.01 | MsG0880045899.01 | -0.818331 | 2.072764e-52 | 1.546989e-49 |
| MsG0380017253.01 | MsG0880045923.01 | -0.822403 | 2.413930e-53 | 2.018559e-50 |
| MsG0380017253.01 | MsG0880046024.01 | -0.802023 | 6.830802e-49 | 3.310802e-46 |
| MsG0380017253.01 | MsG0880046025.01 | -0.811813 | 5.801245e-51 | 3.626782e-48 |
| MsG0280006587.01 | MsG0380017253.01 | -0.835522 | 1.598094e-56 | 1.959067e-53 |
| MsG0280008715.01 | MsG0380017253.01 | -0.811879 | 5.613927e-51 | 3.515748e-48 |
| MsG0280008717.01 | MsG0380017253.01 | -0.802889 | 4.527769e-49 | 2.243599e-46 |
| MsG0280008939.01 | MsG0380017253.01 | -0.829538 | 4.868160e-55 | 4.996753e-52 |
| MsG0280008966.01 | MsG0380017253.01 | -0.816957 | 4.229840e-52 | 3.040378e-49 |
| MsG0280009869.01 | MsG0380017253.01 | -0.819813 | 9.538428e-53 | 7.417589e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017253.01.T01 | MTR_3g103960 | 94.964 | 278 | 12 | 1 | 1 | 276 | 1 | 278 | 0.0 | 545 |
| MsG0380017253.01.T01 | MTR_8g102100 | 44.479 | 326 | 107 | 9 | 24 | 276 | 52 | 376 | 2.17e-76 | 237 |
| MsG0380017253.01.T01 | MTR_6g090380 | 44.918 | 305 | 112 | 9 | 23 | 275 | 76 | 376 | 4.16e-74 | 231 |
| MsG0380017253.01.T01 | MTR_5g071070 | 64.912 | 114 | 39 | 1 | 43 | 156 | 138 | 250 | 1.03e-52 | 181 |
| MsG0380017253.01.T01 | MTR_4g078660 | 66.972 | 109 | 35 | 1 | 49 | 157 | 56 | 163 | 1.99e-52 | 174 |
| MsG0380017253.01.T01 | MTR_5g071070 | 57.664 | 137 | 51 | 2 | 43 | 173 | 220 | 355 | 4.33e-52 | 181 |
| MsG0380017253.01.T01 | MTR_7g092070 | 60.294 | 136 | 50 | 2 | 43 | 175 | 254 | 388 | 4.91e-52 | 181 |
| MsG0380017253.01.T01 | MTR_2g090605 | 68.571 | 105 | 32 | 1 | 53 | 157 | 77 | 180 | 7.56e-51 | 172 |
| MsG0380017253.01.T01 | MTR_3g070880 | 49.704 | 169 | 81 | 2 | 16 | 181 | 126 | 293 | 3.29e-49 | 170 |
| MsG0380017253.01.T01 | MTR_3g029590 | 48.876 | 178 | 76 | 5 | 43 | 219 | 254 | 417 | 4.53e-49 | 173 |
| MsG0380017253.01.T01 | MTR_5g082830 | 64.035 | 114 | 40 | 1 | 43 | 156 | 207 | 319 | 5.25e-48 | 169 |
| MsG0380017253.01.T01 | MTR_1g075890 | 59.690 | 129 | 50 | 2 | 43 | 171 | 219 | 345 | 5.53e-47 | 166 |
| MsG0380017253.01.T01 | MTR_3g435680 | 53.383 | 133 | 54 | 2 | 31 | 156 | 122 | 253 | 1.12e-45 | 158 |
| MsG0380017253.01.T01 | MTR_1g050438 | 60.550 | 109 | 42 | 1 | 48 | 156 | 223 | 330 | 1.71e-45 | 161 |
| MsG0380017253.01.T01 | MTR_0728s0020 | 60.550 | 109 | 42 | 1 | 48 | 156 | 239 | 346 | 2.95e-45 | 161 |
| MsG0380017253.01.T01 | MTR_3g435590 | 37.398 | 246 | 138 | 6 | 43 | 276 | 228 | 469 | 1.58e-44 | 157 |
| MsG0380017253.01.T01 | MTR_3g464270 | 44.872 | 156 | 82 | 2 | 52 | 207 | 8 | 159 | 1.77e-40 | 144 |
| MsG0380017253.01.T01 | MTR_3g464260 | 45.270 | 148 | 45 | 4 | 39 | 177 | 183 | 303 | 3.05e-29 | 116 |
| MsG0380017253.01.T01 | MTR_3g464290 | 56.977 | 86 | 35 | 2 | 43 | 127 | 90 | 174 | 2.09e-27 | 110 |
| MsG0380017253.01.T01 | MTR_3g464280 | 31.250 | 192 | 67 | 4 | 39 | 202 | 165 | 319 | 9.60e-22 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017253.01.T01 | AT4G29190 | 45.833 | 288 | 100 | 10 | 43 | 275 | 69 | 355 | 7.49e-69 | 217 |
| MsG0380017253.01.T01 | AT2G19810 | 42.492 | 313 | 120 | 8 | 22 | 275 | 47 | 358 | 9.18e-69 | 217 |
| MsG0380017253.01.T01 | AT2G25900 | 47.660 | 235 | 89 | 6 | 49 | 256 | 86 | 313 | 9.13e-65 | 205 |
| MsG0380017253.01.T01 | AT2G25900 | 47.660 | 235 | 89 | 6 | 49 | 256 | 86 | 313 | 9.13e-65 | 205 |
| MsG0380017253.01.T01 | AT1G03790 | 51.205 | 166 | 78 | 1 | 12 | 177 | 44 | 206 | 4.38e-56 | 185 |
| MsG0380017253.01.T01 | AT5G44260 | 63.636 | 121 | 41 | 1 | 37 | 157 | 47 | 164 | 1.54e-53 | 178 |
| MsG0380017253.01.T01 | AT2G40140 | 44.053 | 227 | 94 | 4 | 43 | 256 | 204 | 410 | 2.12e-52 | 180 |
| MsG0380017253.01.T01 | AT2G40140 | 44.053 | 227 | 94 | 4 | 43 | 256 | 204 | 410 | 2.12e-52 | 180 |
| MsG0380017253.01.T01 | AT2G41900 | 60.902 | 133 | 50 | 2 | 43 | 175 | 251 | 381 | 8.10e-51 | 177 |
| MsG0380017253.01.T01 | AT5G58620 | 63.158 | 114 | 41 | 1 | 43 | 156 | 199 | 311 | 3.16e-50 | 174 |
| MsG0380017253.01.T01 | AT5G12850 | 62.281 | 114 | 42 | 1 | 43 | 156 | 247 | 359 | 1.40e-49 | 174 |
| MsG0380017253.01.T01 | AT3G55980 | 55.147 | 136 | 60 | 1 | 43 | 178 | 201 | 335 | 6.90e-49 | 170 |
| MsG0380017253.01.T01 | AT3G55980 | 55.147 | 136 | 60 | 1 | 43 | 178 | 268 | 402 | 2.45e-48 | 170 |
| MsG0380017253.01.T01 | AT5G07500 | 60.177 | 113 | 44 | 1 | 48 | 160 | 47 | 158 | 1.15e-47 | 159 |
Find 80 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTGTGTTGTGTTCCATTT+AGG | 0.148827 | 3:-94081314 | None:intergenic |
| GCCAAAATCTATGCAAAATT+TGG | 0.227605 | 3:+94082019 | MsG0380017253.01.T01:CDS |
| TTGGCTTCATGAGGAGTGTT+AGG | 0.270195 | 3:+94081867 | MsG0380017253.01.T01:CDS |
| TGCCCGGAGTTTCGCAAATT+AGG | 0.270671 | 3:+94081581 | MsG0380017253.01.T01:CDS |
| TCCGCAACCCATCCAATATC+AGG | 0.272913 | 3:-94082082 | None:intergenic |
| TCTCAGATGGGTTCTGTGTT+TGG | 0.301160 | 3:+94081911 | MsG0380017253.01.T01:CDS |
| ATTGTGTTGTGTTCCATTTA+GGG | 0.312660 | 3:-94081313 | None:intergenic |
| CAAACCTTAAAATCCCTAAA+TGG | 0.341825 | 3:+94081300 | MsG0380017253.01.T01:CDS |
| TCGGTCCCTGTCCCTGATAT+TGG | 0.350163 | 3:+94082070 | MsG0380017253.01.T01:CDS |
| GTAACGACGGAACCCTTTGC+CGG | 0.351954 | 3:+94081690 | MsG0380017253.01.T01:CDS |
| AAACTATGAAGGGGTTTCTA+TGG | 0.361016 | 3:+94081967 | MsG0380017253.01.T01:CDS |
| TGAATTCGAACATTCGGAAT+TGG | 0.372764 | 3:-94081442 | None:intergenic |
| CTCAAACGATGGTGTTGTTT+CGG | 0.374036 | 3:+94082051 | MsG0380017253.01.T01:CDS |
| GGAGATGAAACAAATGATTC+TGG | 0.374623 | 3:-94081773 | None:intergenic |
| AAACTCCGGGCAAGGATTGC+CGG | 0.383876 | 3:-94081570 | None:intergenic |
| TGGCTTCATGAGGAGTGTTA+GGG | 0.417289 | 3:+94081868 | MsG0380017253.01.T01:CDS |
| GAAACCCCTTCATAGTTTGA+TGG | 0.429345 | 3:-94081962 | None:intergenic |
| CTATGTCATTTGAAATGAAA+TGG | 0.449117 | 3:-94081358 | None:intergenic |
| TCTGTGTTTGGTTCTCCACC+AGG | 0.450591 | 3:+94081923 | MsG0380017253.01.T01:CDS |
| GAACTTTGAATTCGAACATT+CGG | 0.452223 | 3:-94081448 | None:intergenic |
| TCCCTGTCCCTGATATTGGA+TGG | 0.452555 | 3:+94082074 | MsG0380017253.01.T01:CDS |
| TCTATGGAGAGGGTGGAATC+AGG | 0.458742 | 3:+94081983 | MsG0380017253.01.T01:CDS |
| AGCAAACTCGTCGCCGGCAA+AGG | 0.462993 | 3:-94081703 | None:intergenic |
| AGTCTTCCATCAAACTATGA+AGG | 0.470405 | 3:+94081956 | MsG0380017253.01.T01:CDS |
| GATTCTTGCCACTTTGCTCA+TGG | 0.472931 | 3:+94081620 | MsG0380017253.01.T01:CDS |
| TGTCCTAATTTGCGAAACTC+CGG | 0.493268 | 3:-94081584 | None:intergenic |
| ACCTCGATACGGTGTCCCTC+CGG | 0.499369 | 3:+94081826 | MsG0380017253.01.T01:CDS |
| GGAGAATCAAGAACTGAAGT+AGG | 0.502308 | 3:-94081794 | None:intergenic |
| CCCTGTCCCTGATATTGGAT+GGG | 0.503425 | 3:+94082075 | MsG0380017253.01.T01:CDS |
| GTCATTTGAAATGAAATGGT+TGG | 0.505715 | 3:-94081354 | None:intergenic |
| GCAAACTCGTCGCCGGCAAA+GGG | 0.507995 | 3:-94081702 | None:intergenic |
| GAAATGAAATGGTTGGAGAA+TGG | 0.518175 | 3:-94081347 | None:intergenic |
| ACTAATTCCCTCACGTTCAC+CGG | 0.518657 | 3:-94081845 | None:intergenic |
| CTACTCCGGCAATCCTTGCC+CGG | 0.523473 | 3:+94081565 | MsG0380017253.01.T01:CDS |
| ACAGAACCCATCTGAGATAC+CGG | 0.527743 | 3:-94081905 | None:intergenic |
| ACCCATCCAATATCAGGGAC+AGG | 0.527836 | 3:-94082076 | None:intergenic |
| ACAAAGCTGAGTTCTGTAAC+GGG | 0.529200 | 3:-94081669 | None:intergenic |
| ATGAAGGGGTTTCTATGGAG+AGG | 0.530332 | 3:+94081972 | MsG0380017253.01.T01:CDS |
| CCCTGATATTGGATGGGTTG+CGG | 0.534129 | 3:+94082081 | MsG0380017253.01.T01:CDS |
| AAAGTGGCAAGAATCACCTT+TGG | 0.537660 | 3:-94081612 | None:intergenic |
| ATGGAGTCCGGTATCTCAGA+TGG | 0.538705 | 3:+94081898 | MsG0380017253.01.T01:CDS |
| GTCCTAATTTGCGAAACTCC+GGG | 0.542282 | 3:-94081583 | None:intergenic |
| GTCTTCCATCAAACTATGAA+GGG | 0.551277 | 3:+94081957 | MsG0380017253.01.T01:CDS |
| GGGAATTAGTTGGCTTCATG+AGG | 0.558865 | 3:+94081858 | MsG0380017253.01.T01:CDS |
| AATTTGCGAAACTCCGGGCA+AGG | 0.560424 | 3:-94081578 | None:intergenic |
| ATTGGTCTGTTGAGAAAACA+TGG | 0.569270 | 3:-94081424 | None:intergenic |
| ACACCGTATCGAGGTTTCCT+CGG | 0.574430 | 3:-94081818 | None:intergenic |
| TAGGGTTGATGAATGGAGTC+CGG | 0.576489 | 3:+94081886 | MsG0380017253.01.T01:CDS |
| TACAAAGCTGAGTTCTGTAA+CGG | 0.577054 | 3:-94081670 | None:intergenic |
| AGGGGTTTCTATGGAGAGGG+TGG | 0.581223 | 3:+94081976 | MsG0380017253.01.T01:CDS |
| GTGTCCCTCCGGTGAACGTG+AGG | 0.581697 | 3:+94081837 | MsG0380017253.01.T01:CDS |
| CAAAGAAGCAAACTCGTCGC+CGG | 0.584023 | 3:-94081709 | None:intergenic |
| GTGAACGTGAGGGAATTAGT+TGG | 0.588119 | 3:+94081848 | MsG0380017253.01.T01:CDS |
| AACGTGGTAGGTCTCATGAC+TGG | 0.591872 | 3:+94081480 | MsG0380017253.01.T01:CDS |
| TCTGTTGAGAAAACATGGAT+TGG | 0.594271 | 3:-94081419 | None:intergenic |
| GGAGTGTTAGGGTTGATGAA+TGG | 0.595988 | 3:+94081879 | MsG0380017253.01.T01:CDS |
| AAATTAGGACACTGCACCAA+AGG | 0.596122 | 3:+94081596 | MsG0380017253.01.T01:CDS |
| TTCGAAAATGCCAACGTGGT+AGG | 0.602152 | 3:+94081468 | MsG0380017253.01.T01:CDS |
| AGCTGAGTTCTGTAACGGGA+AGG | 0.612629 | 3:-94081665 | None:intergenic |
| GTGTTTGGTTCTCCACCAGG+TGG | 0.617183 | 3:+94081926 | MsG0380017253.01.T01:CDS |
| TGGAGTCCGGTATCTCAGAT+GGG | 0.620816 | 3:+94081899 | MsG0380017253.01.T01:CDS |
| CCGCAACCCATCCAATATCA+GGG | 0.621198 | 3:-94082081 | None:intergenic |
| TCTTCCATCAAACTATGAAG+GGG | 0.627249 | 3:+94081958 | MsG0380017253.01.T01:CDS |
| GGTAGATTGAACTCAAACGA+TGG | 0.627699 | 3:+94082040 | MsG0380017253.01.T01:CDS |
| ATTCCCTCACGTTCACCGGA+GGG | 0.629594 | 3:-94081841 | None:intergenic |
| CCTCCGAGGAAACCTCGATA+CGG | 0.630315 | 3:+94081815 | MsG0380017253.01.T01:CDS |
| AGAACTCAGCTTTGTAACGA+CGG | 0.632022 | 3:+94081677 | MsG0380017253.01.T01:CDS |
| GTGAAGCGTTATTTGAGACA+CGG | 0.632246 | 3:-94081751 | None:intergenic |
| CAGTTCTTGATTCTCCTCCG+AGG | 0.634516 | 3:+94081801 | MsG0380017253.01.T01:CDS |
| AATTCCCTCACGTTCACCGG+AGG | 0.638441 | 3:-94081842 | None:intergenic |
| GATTGTCCATACTCTCATCC+CGG | 0.639508 | 3:+94081506 | MsG0380017253.01.T01:CDS |
| TGAAGGGGTTTCTATGGAGA+GGG | 0.646493 | 3:+94081973 | MsG0380017253.01.T01:CDS |
| CCCATCCAATATCAGGGACA+GGG | 0.654742 | 3:-94082075 | None:intergenic |
| TGTCCCTCCGGTGAACGTGA+GGG | 0.657063 | 3:+94081838 | MsG0380017253.01.T01:CDS |
| TGGTAGGTCTCATGACTGGA+CGG | 0.659097 | 3:+94081484 | MsG0380017253.01.T01:CDS |
| ATGGAACACAACACAATACG+AGG | 0.669458 | 3:+94081319 | MsG0380017253.01.T01:CDS |
| ACCGGAGGGACACCGTATCG+AGG | 0.671631 | 3:-94081827 | None:intergenic |
| AGTCATGAGACCTACCACGT+TGG | 0.672854 | 3:-94081478 | None:intergenic |
| CCGTATCGAGGTTTCCTCGG+AGG | 0.710192 | 3:-94081815 | None:intergenic |
| AAAGTTCGAAAATGCCAACG+TGG | 0.760484 | 3:+94081464 | MsG0380017253.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACCAAATTTTGCATAGATTT+TGG | - | Chr3:94082023-94082042 | None:intergenic | 25.0% |
| !! | CTATGTCATTTGAAATGAAA+TGG | - | Chr3:94081361-94081380 | None:intergenic | 25.0% |
| ATTGTGTTGTGTTCCATTTA+GGG | - | Chr3:94081316-94081335 | None:intergenic | 30.0% | |
| CAAACCTTAAAATCCCTAAA+TGG | + | Chr3:94081300-94081319 | MsG0380017253.01.T01:CDS | 30.0% | |
| GAACTTTGAATTCGAACATT+CGG | - | Chr3:94081451-94081470 | None:intergenic | 30.0% | |
| GCCAAAATCTATGCAAAATT+TGG | + | Chr3:94082019-94082038 | MsG0380017253.01.T01:CDS | 30.0% | |
| TATTGTGTTGTGTTCCATTT+AGG | - | Chr3:94081317-94081336 | None:intergenic | 30.0% | |
| ! | GTCATTTGAAATGAAATGGT+TGG | - | Chr3:94081357-94081376 | None:intergenic | 30.0% |
| ! | TGTTCCATTTAGGGATTTTA+AGG | - | Chr3:94081307-94081326 | None:intergenic | 30.0% |
| !!! | CATTTAGGGATTTTAAGGTT+TGG | - | Chr3:94081302-94081321 | None:intergenic | 30.0% |
| AAACTATGAAGGGGTTTCTA+TGG | + | Chr3:94081967-94081986 | MsG0380017253.01.T01:CDS | 35.0% | |
| AGTCTTCCATCAAACTATGA+AGG | + | Chr3:94081956-94081975 | MsG0380017253.01.T01:CDS | 35.0% | |
| CCTCAAAAATACAACTACTC+CGG | + | Chr3:94081551-94081570 | MsG0380017253.01.T01:CDS | 35.0% | |
| GAAATGAAATGGTTGGAGAA+TGG | - | Chr3:94081350-94081369 | None:intergenic | 35.0% | |
| GGAGATGAAACAAATGATTC+TGG | - | Chr3:94081776-94081795 | None:intergenic | 35.0% | |
| GTCTTCCATCAAACTATGAA+GGG | + | Chr3:94081957-94081976 | MsG0380017253.01.T01:CDS | 35.0% | |
| TACAAAGCTGAGTTCTGTAA+CGG | - | Chr3:94081673-94081692 | None:intergenic | 35.0% | |
| TCTGTTGAGAAAACATGGAT+TGG | - | Chr3:94081422-94081441 | None:intergenic | 35.0% | |
| TCTTCCATCAAACTATGAAG+GGG | + | Chr3:94081958-94081977 | MsG0380017253.01.T01:CDS | 35.0% | |
| TGAATTCGAACATTCGGAAT+TGG | - | Chr3:94081445-94081464 | None:intergenic | 35.0% | |
| ! | ATTGGTCTGTTGAGAAAACA+TGG | - | Chr3:94081427-94081446 | None:intergenic | 35.0% |
| !! | CGGAGTAGTTGTATTTTTGA+GGG | - | Chr3:94081553-94081572 | None:intergenic | 35.0% |
| AAAGTGGCAAGAATCACCTT+TGG | - | Chr3:94081615-94081634 | None:intergenic | 40.0% | |
| AAAGTTCGAAAATGCCAACG+TGG | + | Chr3:94081464-94081483 | MsG0380017253.01.T01:CDS | 40.0% | |
| AAATTAGGACACTGCACCAA+AGG | + | Chr3:94081596-94081615 | MsG0380017253.01.T01:CDS | 40.0% | |
| ACAAAGCTGAGTTCTGTAAC+GGG | - | Chr3:94081672-94081691 | None:intergenic | 40.0% | |
| ATGGAACACAACACAATACG+AGG | + | Chr3:94081319-94081338 | MsG0380017253.01.T01:CDS | 40.0% | |
| CAAAAACACCATGAGCAAAG+TGG | - | Chr3:94081631-94081650 | None:intergenic | 40.0% | |
| GAAACCCCTTCATAGTTTGA+TGG | - | Chr3:94081965-94081984 | None:intergenic | 40.0% | |
| GGAGAATCAAGAACTGAAGT+AGG | - | Chr3:94081797-94081816 | None:intergenic | 40.0% | |
| GGTAGATTGAACTCAAACGA+TGG | + | Chr3:94082040-94082059 | MsG0380017253.01.T01:CDS | 40.0% | |
| ! | AGAACTCAGCTTTGTAACGA+CGG | + | Chr3:94081677-94081696 | MsG0380017253.01.T01:CDS | 40.0% |
| ! | GTGAAGCGTTATTTGAGACA+CGG | - | Chr3:94081754-94081773 | None:intergenic | 40.0% |
| ! | TGTCCTAATTTGCGAAACTC+CGG | - | Chr3:94081587-94081606 | None:intergenic | 40.0% |
| !! | CCGGAGTAGTTGTATTTTTG+AGG | - | Chr3:94081554-94081573 | None:intergenic | 40.0% |
| !! | CTCAAACGATGGTGTTGTTT+CGG | + | Chr3:94082051-94082070 | MsG0380017253.01.T01:CDS | 40.0% |
| !! | CTCATGGTGTTTTTGAATGC+TGG | + | Chr3:94081636-94081655 | MsG0380017253.01.T01:CDS | 40.0% |
| !! | TGTATTTTTGAGGGTCTCTC+CGG | - | Chr3:94081544-94081563 | None:intergenic | 40.0% |
| ACTAATTCCCTCACGTTCAC+CGG | - | Chr3:94081848-94081867 | None:intergenic | 45.0% | |
| AGACTCAAAAAACCACCTGG+TGG | - | Chr3:94081941-94081960 | None:intergenic | 45.0% | |
| ATGAAGGGGTTTCTATGGAG+AGG | + | Chr3:94081972-94081991 | MsG0380017253.01.T01:CDS | 45.0% | |
| GATTGTCCATACTCTCATCC+CGG | + | Chr3:94081506-94081525 | MsG0380017253.01.T01:CDS | 45.0% | |
| GGAAGACTCAAAAAACCACC+TGG | - | Chr3:94081944-94081963 | None:intergenic | 45.0% | |
| GTGAACGTGAGGGAATTAGT+TGG | + | Chr3:94081848-94081867 | MsG0380017253.01.T01:CDS | 45.0% | |
| TGAAGGGGTTTCTATGGAGA+GGG | + | Chr3:94081973-94081992 | MsG0380017253.01.T01:CDS | 45.0% | |
| TTCGAAAATGCCAACGTGGT+AGG | + | Chr3:94081468-94081487 | MsG0380017253.01.T01:CDS | 45.0% | |
| ! | ACAGAACCCATCTGAGATAC+CGG | - | Chr3:94081908-94081927 | None:intergenic | 45.0% |
| ! | GGGAATTAGTTGGCTTCATG+AGG | + | Chr3:94081858-94081877 | MsG0380017253.01.T01:CDS | 45.0% |
| ! | GTCCTAATTTGCGAAACTCC+GGG | - | Chr3:94081586-94081605 | None:intergenic | 45.0% |
| ! | TCTCAGATGGGTTCTGTGTT+TGG | + | Chr3:94081911-94081930 | MsG0380017253.01.T01:CDS | 45.0% |
| !! | GATTCTTGCCACTTTGCTCA+TGG | + | Chr3:94081620-94081639 | MsG0380017253.01.T01:CDS | 45.0% |
| !! | GGAGTGTTAGGGTTGATGAA+TGG | + | Chr3:94081879-94081898 | MsG0380017253.01.T01:CDS | 45.0% |
| !! | TAGGGTTGATGAATGGAGTC+CGG | + | Chr3:94081886-94081905 | MsG0380017253.01.T01:CDS | 45.0% |
| !! | TGGCTTCATGAGGAGTGTTA+GGG | + | Chr3:94081868-94081887 | MsG0380017253.01.T01:CDS | 45.0% |
| !! | TTGGCTTCATGAGGAGTGTT+AGG | + | Chr3:94081867-94081886 | MsG0380017253.01.T01:CDS | 45.0% |
| AACGTGGTAGGTCTCATGAC+TGG | + | Chr3:94081480-94081499 | MsG0380017253.01.T01:CDS | 50.0% | |
| AATTTGCGAAACTCCGGGCA+AGG | - | Chr3:94081581-94081600 | None:intergenic | 50.0% | |
| ACACCGTATCGAGGTTTCCT+CGG | - | Chr3:94081821-94081840 | None:intergenic | 50.0% | |
| ACCCATCCAATATCAGGGAC+AGG | - | Chr3:94082079-94082098 | None:intergenic | 50.0% | |
| AGTCATGAGACCTACCACGT+TGG | - | Chr3:94081481-94081500 | None:intergenic | 50.0% | |
| ATGGAGTCCGGTATCTCAGA+TGG | + | Chr3:94081898-94081917 | MsG0380017253.01.T01:CDS | 50.0% | |
| CAAAGAAGCAAACTCGTCGC+CGG | - | Chr3:94081712-94081731 | None:intergenic | 50.0% | |
| CAGTTCTTGATTCTCCTCCG+AGG | + | Chr3:94081801-94081820 | MsG0380017253.01.T01:CDS | 50.0% | |
| CCCATCCAATATCAGGGACA+GGG | - | Chr3:94082078-94082097 | None:intergenic | 50.0% | |
| CCCTGTCCCTGATATTGGAT+GGG | + | Chr3:94082075-94082094 | MsG0380017253.01.T01:CDS | 50.0% | |
| CCGCAACCCATCCAATATCA+GGG | - | Chr3:94082084-94082103 | None:intergenic | 50.0% | |
| TCCCTGTCCCTGATATTGGA+TGG | + | Chr3:94082074-94082093 | MsG0380017253.01.T01:CDS | 50.0% | |
| TCCGCAACCCATCCAATATC+AGG | - | Chr3:94082085-94082104 | None:intergenic | 50.0% | |
| TCTATGGAGAGGGTGGAATC+AGG | + | Chr3:94081983-94082002 | MsG0380017253.01.T01:CDS | 50.0% | |
| TGCCCGGAGTTTCGCAAATT+AGG | + | Chr3:94081581-94081600 | MsG0380017253.01.T01:CDS | 50.0% | |
| TGGAGTCCGGTATCTCAGAT+GGG | + | Chr3:94081899-94081918 | MsG0380017253.01.T01:CDS | 50.0% | |
| TGGTAGGTCTCATGACTGGA+CGG | + | Chr3:94081484-94081503 | MsG0380017253.01.T01:CDS | 50.0% | |
| ! | AGCTGAGTTCTGTAACGGGA+AGG | - | Chr3:94081668-94081687 | None:intergenic | 50.0% |
| ! | TTTTCGCCGGGATGAGAGTA+TGG | - | Chr3:94081515-94081534 | None:intergenic | 50.0% |
| !! | CCCTGATATTGGATGGGTTG+CGG | + | Chr3:94082081-94082100 | MsG0380017253.01.T01:CDS | 50.0% |
| !! | TCTGTGTTTGGTTCTCCACC+AGG | + | Chr3:94081923-94081942 | MsG0380017253.01.T01:CDS | 50.0% |
| AAACTCCGGGCAAGGATTGC+CGG | - | Chr3:94081573-94081592 | None:intergenic | 55.0% | |
| AATTCCCTCACGTTCACCGG+AGG | - | Chr3:94081845-94081864 | None:intergenic | 55.0% | |
| AGGGGTTTCTATGGAGAGGG+TGG | + | Chr3:94081976-94081995 | MsG0380017253.01.T01:CDS | 55.0% | |
| ATTCCCTCACGTTCACCGGA+GGG | - | Chr3:94081844-94081863 | None:intergenic | 55.0% | |
| CCTCCGAGGAAACCTCGATA+CGG | + | Chr3:94081815-94081834 | MsG0380017253.01.T01:CDS | 55.0% | |
| GTAACGACGGAACCCTTTGC+CGG | + | Chr3:94081690-94081709 | MsG0380017253.01.T01:CDS | 55.0% | |
| TCGGTCCCTGTCCCTGATAT+TGG | + | Chr3:94082070-94082089 | MsG0380017253.01.T01:CDS | 55.0% | |
| !! | GTGTTTGGTTCTCCACCAGG+TGG | + | Chr3:94081926-94081945 | MsG0380017253.01.T01:CDS | 55.0% |
| ACCTCGATACGGTGTCCCTC+CGG | + | Chr3:94081826-94081845 | MsG0380017253.01.T01:CDS | 60.0% | |
| AGCAAACTCGTCGCCGGCAA+AGG | - | Chr3:94081706-94081725 | None:intergenic | 60.0% | |
| ATCCCGGCGAAAAAGCTCGC+CGG | + | Chr3:94081522-94081541 | MsG0380017253.01.T01:CDS | 60.0% | |
| CCGTATCGAGGTTTCCTCGG+AGG | - | Chr3:94081818-94081837 | None:intergenic | 60.0% | |
| CTACTCCGGCAATCCTTGCC+CGG | + | Chr3:94081565-94081584 | MsG0380017253.01.T01:CDS | 60.0% | |
| GCAAACTCGTCGCCGGCAAA+GGG | - | Chr3:94081705-94081724 | None:intergenic | 60.0% | |
| TGTCCCTCCGGTGAACGTGA+GGG | + | Chr3:94081838-94081857 | MsG0380017253.01.T01:CDS | 60.0% | |
| !!! | TCTCCGGCGAGCTTTTTCGC+CGG | - | Chr3:94081528-94081547 | None:intergenic | 60.0% |
| ACCGGAGGGACACCGTATCG+AGG | - | Chr3:94081830-94081849 | None:intergenic | 65.0% | |
| GTGTCCCTCCGGTGAACGTG+AGG | + | Chr3:94081837-94081856 | MsG0380017253.01.T01:CDS | 65.0% | |
| !!! | CTCCGGCGAGCTTTTTCGCC+GGG | - | Chr3:94081527-94081546 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 94081287 | 94082117 | 94081287 | ID=MsG0380017253.01;Name=MsG0380017253.01 |
| Chr3 | mRNA | 94081287 | 94082117 | 94081287 | ID=MsG0380017253.01.T01;Parent=MsG0380017253.01;Name=MsG0380017253.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|276 |
| Chr3 | exon | 94081287 | 94082117 | 94081287 | ID=MsG0380017253.01.T01:exon:26883;Parent=MsG0380017253.01.T01 |
| Chr3 | CDS | 94081287 | 94082117 | 94081287 | ID=MsG0380017253.01.T01:cds;Parent=MsG0380017253.01.T01 |
| Gene Sequence |
| Protein sequence |
