AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026026.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026026.01.T01 MTR_5g037890 96.032 126 5 0 1 126 1 126 1.72e-90 258
MsG0580026026.01.T01 MTR_5g037950 65.873 126 42 1 1 125 1 126 2.44e-51 159
MsG0580026026.01.T01 MTR_5g037930 64.062 128 44 1 1 126 1 128 4.29e-50 155
MsG0580026026.01.T01 MTR_7g009070 53.774 106 49 0 20 125 28 133 6.31e-42 135
MsG0580026026.01.T01 MTR_3g112410 46.847 111 59 0 14 124 2 112 2.15e-36 120
MsG0580026026.01.T01 MTR_1g023140 40.708 113 67 0 14 126 2 114 1.74e-33 113
MsG0580026026.01.T01 MTR_5g021180 42.342 111 64 0 14 124 4 114 2.79e-33 113
MsG0580026026.01.T01 MTR_8g107460 41.346 104 61 0 18 121 254 357 1.84e-24 95.9
MsG0580026026.01.T01 MTR_8g005320 32.381 105 71 0 17 121 25 129 3.04e-22 85.5
MsG0580026026.01.T01 MTR_7g073680 34.286 105 69 0 17 121 23 127 4.11e-22 85.1
MsG0580026026.01.T01 MTR_2g010750 37.143 105 66 0 18 122 9 113 1.07e-21 84.0
MsG0580026026.01.T01 MTR_8g006685 32.381 105 71 0 17 121 94 198 5.38e-21 84.3
MsG0580026026.01.T01 MTR_8g006560 32.381 105 71 0 17 121 94 198 5.38e-21 84.3
MsG0580026026.01.T01 MTR_4g081380 35.514 107 65 2 18 122 10 114 9.65e-20 79.0
MsG0580026026.01.T01 MTR_2g082590 30.476 105 73 0 18 122 10 114 1.90e-18 75.5
MsG0580026026.01.T01 MTR_2g082590 31.858 113 68 2 10 122 24 127 3.50e-18 75.1
MsG0580026026.01.T01 MTR_1g098660 32.222 90 59 2 39 126 77 166 1.54e-14 66.6
MsG0580026026.01.T01 MTR_8g059015 34.091 88 56 2 38 123 87 174 4.91e-14 65.5
MsG0580026026.01.T01 MTR_7g080250 36.364 88 55 1 40 126 94 181 5.01e-14 65.5
MsG0580026026.01.T01 MTR_3g089065 27.586 116 79 3 10 123 59 171 8.09e-14 64.7
MsG0580026026.01.T01 MTR_4g094690 32.927 82 53 1 44 123 85 166 1.11e-13 64.3
MsG0580026026.01.T01 MTR_2g079420 34.091 88 56 2 38 123 84 171 4.01e-13 62.8
MsG0580026026.01.T01 MTR_1g114290 32.911 79 49 2 38 115 84 159 4.92e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026026.01.T01 AT5G39950 46.847 111 59 0 15 125 22 132 8.19e-41 132
MsG0580026026.01.T01 AT1G69880 46.729 107 57 0 17 123 36 142 7.91e-35 117
MsG0580026026.01.T01 AT1G59730 39.844 128 74 1 1 125 1 128 1.09e-33 114
MsG0580026026.01.T01 AT3G51030 41.818 110 64 0 14 123 2 111 1.05e-32 111
MsG0580026026.01.T01 AT1G19730 44.737 114 62 1 14 126 2 115 9.21e-32 109
MsG0580026026.01.T01 AT1G45145 42.991 107 61 0 18 124 5 111 1.05e-30 106
MsG0580026026.01.T01 AT5G42980 42.727 110 63 0 17 126 4 113 2.14e-30 105
MsG0580026026.01.T01 AT3G17880 39.806 103 62 0 19 121 264 366 5.27e-22 89.7
MsG0580026026.01.T01 AT3G17880 39.806 103 62 0 19 121 271 373 6.05e-22 89.4
MsG0580026026.01.T01 AT3G56420 35.870 92 59 0 28 119 50 141 3.46e-20 80.9
MsG0580026026.01.T01 AT3G56420 35.870 92 59 0 28 119 50 141 3.46e-20 80.9
MsG0580026026.01.T01 AT3G56420 35.870 92 59 0 28 119 50 141 3.46e-20 80.9
MsG0580026026.01.T01 AT3G08710 33.333 93 62 0 29 121 34 126 4.54e-19 77.4
MsG0580026026.01.T01 AT3G08710 33.333 93 62 0 29 121 34 126 4.54e-19 77.4
MsG0580026026.01.T01 AT3G08710 33.333 93 62 0 29 121 34 126 4.54e-19 77.4
MsG0580026026.01.T01 AT1G11530 32.381 105 71 0 18 122 2 106 1.19e-17 73.2
MsG0580026026.01.T01 AT2G40790 33.696 92 61 0 28 119 50 141 3.58e-16 70.5
MsG0580026026.01.T01 AT2G40790 33.696 92 61 0 28 119 50 141 3.58e-16 70.5
MsG0580026026.01.T01 AT1G43560 28.125 128 86 3 1 122 33 160 1.41e-15 69.3
MsG0580026026.01.T01 AT5G16400 37.500 88 54 1 40 126 97 184 2.43e-15 68.9
MsG0580026026.01.T01 AT3G02730 39.080 87 52 1 41 126 88 174 4.30e-15 68.2
MsG0580026026.01.T01 AT4G03520 38.554 83 49 2 43 123 101 183 8.00e-15 67.8
MsG0580026026.01.T01 AT1G03680 38.272 81 48 2 45 123 97 177 2.16e-14 66.2
MsG0580026026.01.T01 AT1G31020 30.909 110 74 1 16 123 47 156 4.52e-14 65.1
MsG0580026026.01.T01 AT1G76760 28.421 95 61 3 33 120 70 164 9.81e-14 64.3
MsG0580026026.01.T01 AT2G35010 36.250 80 47 2 47 123 113 191 9.17e-13 62.4
MsG0580026026.01.T01 AT2G35010 36.250 80 47 2 47 123 113 191 9.17e-13 62.4
MsG0580026026.01.T01 AT1G50320 36.709 79 46 2 38 115 85 160 3.33e-12 60.8
MsG0580026026.01.T01 AT1G52990 31.868 91 60 2 34 123 222 311 1.80e-11 60.1

Find 22 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCATTCCACTGCTAAA+TGG 0.236688 5:-29320755 MsG0580026026.01.T01:CDS
GCAGCAAAATCTTTCATAAC+TGG 0.263663 5:+29320553 None:intergenic
GCTTGATGTTGATGAGTTAT+TGG 0.264813 5:-29320503 MsG0580026026.01.T01:intron
TTATTGATTTCACAGCTAAA+TGG 0.322109 5:-29320600 MsG0580026026.01.T01:CDS
TTTCAAAGAAACTAATAAAC+TGG 0.393712 5:-29320715 MsG0580026026.01.T01:intron
TTCTTAATGAGTAGAAAAGT+TGG 0.438267 5:+29320252 None:intergenic
AGGAAAAGTTGTTGATAAAG+TGG 0.478902 5:-29320229 MsG0580026026.01.T01:CDS
TAAAGTGGTGGGAGTAAGAA+AGG 0.479129 5:-29320214 MsG0580026026.01.T01:CDS
GGGAGTTTCTCAAACATTCC+AGG 0.482800 5:-29320286 MsG0580026026.01.T01:CDS
GTGTGGACCTTGCAAAGTTA+TGG 0.487071 5:-29320578 MsG0580026026.01.T01:CDS
ATACACAAAATTATACAAGT+TGG 0.527315 5:+29320812 None:intergenic
CATTCCATTTAGCAGTGGAA+TGG 0.530623 5:+29320751 None:intergenic
TGGAATGGAATGCAAGGACA+TGG 0.540723 5:+29320766 None:intergenic
AAAAGTTGTTGATAAAGTGG+TGG 0.577415 5:-29320226 MsG0580026026.01.T01:CDS
AAGTTGGCAATGAATGCACC+TGG 0.579245 5:+29320268 None:intergenic
GATTTCACAGCTAAATGGTG+TGG 0.590907 5:-29320595 MsG0580026026.01.T01:CDS
AAAGTTGTTGATAAAGTGGT+GGG 0.597105 5:-29320225 MsG0580026026.01.T01:CDS
ACTGGATCCATAACTTTGCA+AGG 0.602265 5:+29320571 None:intergenic
ATGAGCATTCCATTTAGCAG+TGG 0.609708 5:+29320746 None:intergenic
TAGCAGTGGAATGGAATGCA+AGG 0.615008 5:+29320760 None:intergenic
TAAGAAAGGAGCAACTACAA+AGG 0.660815 5:-29320200 MsG0580026026.01.T01:CDS
GGAATGGAATGCAAGGACAT+GGG 0.705076 5:+29320767 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAGCATTTATAAT+AGG + Chr5:29320627-29320646 None:intergenic 10.0%
!! ATACACAAAATTATACAAGT+TGG + Chr5:29320186-29320205 None:intergenic 20.0%
!! TTTCAAAGAAACTAATAAAC+TGG - Chr5:29320280-29320299 MsG0580026026.01.T01:CDS 20.0%
!!! TCTTATGTAGTATTTTTCTT+TGG - Chr5:29320363-29320382 MsG0580026026.01.T01:intron 20.0%
!!! TGTTTTTTCCACAATATAAA+TGG - Chr5:29320681-29320700 MsG0580026026.01.T01:intron 20.0%
! TAACAAACTAGTTAAAGTGA+AGG + Chr5:29320533-29320552 None:intergenic 25.0%
! TTCTTAATGAGTAGAAAAGT+TGG + Chr5:29320746-29320765 None:intergenic 25.0%
!! ACTTTTCTACTCATTAAGAA+AGG - Chr5:29320746-29320765 MsG0580026026.01.T01:CDS 25.0%
!! GTATTTTTCTTTGGTATAGA+TGG - Chr5:29320372-29320391 MsG0580026026.01.T01:intron 25.0%
!!! TTATTGATTTCACAGCTAAA+TGG - Chr5:29320395-29320414 MsG0580026026.01.T01:intron 25.0%
AATTAAACCTGGCATAAAGT+TGG + Chr5:29320567-29320586 None:intergenic 30.0%
! AAAAGTTGTTGATAAAGTGG+TGG - Chr5:29320769-29320788 MsG0580026026.01.T01:CDS 30.0%
! AAAGTTGTTGATAAAGTGGT+GGG - Chr5:29320770-29320789 MsG0580026026.01.T01:CDS 30.0%
! AGGAAAAGTTGTTGATAAAG+TGG - Chr5:29320766-29320785 MsG0580026026.01.T01:CDS 30.0%
ACCAACTGAGTAATTAAACC+TGG + Chr5:29320578-29320597 None:intergenic 35.0%
CCACAATATAAATGGCAACA+GGG - Chr5:29320689-29320708 MsG0580026026.01.T01:intron 35.0%
GCAGCAAAATCTTTCATAAC+TGG + Chr5:29320445-29320464 None:intergenic 35.0%
TAAGAAAGGAGCAACTACAA+AGG - Chr5:29320795-29320814 MsG0580026026.01.T01:CDS 35.0%
TCCACAATATAAATGGCAAC+AGG - Chr5:29320688-29320707 MsG0580026026.01.T01:intron 35.0%
TGTAAAACCAACTTTATGCC+AGG - Chr5:29320557-29320576 MsG0580026026.01.T01:CDS 35.0%
! GCTTGATGTTGATGAGTTAT+TGG - Chr5:29320492-29320511 MsG0580026026.01.T01:intron 35.0%
ACTGGATCCATAACTTTGCA+AGG + Chr5:29320427-29320446 None:intergenic 40.0%
ATGAGCATTCCATTTAGCAG+TGG + Chr5:29320252-29320271 None:intergenic 40.0%
CATTCCATTCCACTGCTAAA+TGG - Chr5:29320240-29320259 MsG0580026026.01.T01:CDS 40.0%
CATTCCATTTAGCAGTGGAA+TGG + Chr5:29320247-29320266 None:intergenic 40.0%
GATTTCACAGCTAAATGGTG+TGG - Chr5:29320400-29320419 MsG0580026026.01.T01:intron 40.0%
GCCAGGTTTAATTACTCAGT+TGG - Chr5:29320574-29320593 MsG0580026026.01.T01:CDS 40.0%
! CCCTGTTGCCATTTATATTG+TGG + Chr5:29320692-29320711 None:intergenic 40.0%
! TAAAGTGGTGGGAGTAAGAA+AGG - Chr5:29320781-29320800 MsG0580026026.01.T01:CDS 40.0%
AAGTTGGCAATGAATGCACC+TGG + Chr5:29320730-29320749 None:intergenic 45.0%
GGAATGGAATGCAAGGACAT+GGG + Chr5:29320231-29320250 None:intergenic 45.0%
GGGAGTTTCTCAAACATTCC+AGG - Chr5:29320709-29320728 MsG0580026026.01.T01:intron 45.0%
GTGTGGACCTTGCAAAGTTA+TGG - Chr5:29320417-29320436 MsG0580026026.01.T01:intron 45.0%
TAGCAGTGGAATGGAATGCA+AGG + Chr5:29320238-29320257 None:intergenic 45.0%
TGGAATGGAATGCAAGGACA+TGG + Chr5:29320232-29320251 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr5 gene 29320176 29320841 29320176 ID=MsG0580026026.01;Name=MsG0580026026.01
Chr5 mRNA 29320176 29320841 29320176 ID=MsG0580026026.01.T01;Parent=MsG0580026026.01;Name=MsG0580026026.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|3|0|126
Chr5 exon 29320716 29320841 29320716 ID=MsG0580026026.01.T01:exon:33463;Parent=MsG0580026026.01.T01
Chr5 exon 29320504 29320626 29320504 ID=MsG0580026026.01.T01:exon:33462;Parent=MsG0580026026.01.T01
Chr5 exon 29320176 29320307 29320176 ID=MsG0580026026.01.T01:exon:33461;Parent=MsG0580026026.01.T01
Chr5 CDS 29320716 29320841 29320716 ID=MsG0580026026.01.T01:cds;Parent=MsG0580026026.01.T01
Chr5 CDS 29320504 29320626 29320504 ID=MsG0580026026.01.T01:cds;Parent=MsG0580026026.01.T01
Chr5 CDS 29320176 29320307 29320176 ID=MsG0580026026.01.T01:cds;Parent=MsG0580026026.01.T01
Gene Sequence

>MsG0580026026.01.T01

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTGCATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTCAAAGAAACTAATAAACTGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTATGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGGAGTTTCTCAAACATTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAAGTTGTTGATAAAGTGGTGGGAGTAAGAAAGGAGCAACTACAAAGGATGATTGAAAAGCAAAGAAACTAA

Protein sequence

>MsG0580026026.01.T01

MSSNLYNFVYVDRSTESSHVLAFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVMKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTFLLIKKGKVVDKVVGVRKEQLQRMIEKQRN*