AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017780.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017780.01.T01 MTR_3g111970 95.652 138 6 0 1 138 1 138 2.42e-96 274
MsG0380017780.01.T01 MTR_3g111970 94.783 115 6 0 1 115 1 115 1.47e-77 225
MsG0380017780.01.T01 MTR_1g022395 60.417 144 48 4 1 137 1 142 1.21e-52 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017780.01.T01 AT1G19530 44.882 127 54 6 1 126 1 112 1.02e-19 79.3
MsG0380017780.01.T01 AT1G19530 44.882 127 54 6 1 126 1 112 1.02e-19 79.3

Find 53 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGAAATTGATGCTTTC+TGG 0.107507 3:+100582336 MsG0380017780.01.T01:CDS
ATTAATTAAGTACCTGATTT+AGG 0.181404 3:-100582208 None:intergenic
CTGAATAGTCCTTGATAAAT+TGG 0.192807 3:-100582402 None:intergenic
GAAAGCATCAATTTCTTCTT+TGG 0.235405 3:-100582333 None:intergenic
CAATTTATCAAGGACTATTC+AGG 0.271260 3:+100582403 MsG0380017780.01.T01:CDS
TTAATTTATCCATAAGGTTT+TGG 0.305558 3:+100583078 MsG0380017780.01.T01:three_prime_UTR
CACGGTATCGTCGTATTTGC+CGG 0.314261 3:-100582541 None:intergenic
TTACATTTATATATTGTAAA+GGG 0.358760 3:-100582970 None:intergenic
AAATAAATACCAAAACCTTA+TGG 0.361279 3:-100583087 None:intergenic
GCAGGTGGACCAAGAGCAAC+TGG 0.363566 3:+100582801 MsG0380017780.01.T01:intron
CGCCCCATGAAGAAATCATT+TGG 0.393233 3:+100582573 MsG0380017780.01.T01:CDS
TTGGTAGGAGCTTCCATCAC+TGG 0.398603 3:-100582842 None:intergenic
CATGCCAAATGATTTCTTCA+TGG 0.402591 3:-100582577 None:intergenic
TCAATCAATGGGTTCTCTAA+TGG 0.403373 3:+100582159 MsG0380017780.01.T01:exon
TTGGAATTAAGAGTGTGATT+TGG 0.408407 3:+100582095 MsG0380017780.01.T01:five_prime_UTR
AAAATGTTAATTTATCCATA+AGG 0.408580 3:+100583072 MsG0380017780.01.T01:three_prime_UTR
TTCTTCATGGGGCGGGCCAA+GGG 0.427383 3:-100582564 None:intergenic
CAGAATCACTTGAAAGGAAT+CGG 0.429094 3:+100582303 MsG0380017780.01.T01:intron
GTTACATTTATATATTGTAA+AGG 0.436853 3:-100582971 None:intergenic
ATGCCAAATGATTTCTTCAT+GGG 0.446330 3:-100582576 None:intergenic
TTTCTTCATGGGGCGGGCCA+AGG 0.451036 3:-100582565 None:intergenic
ATAAATTGGAAATAACTTTG+AGG 0.453495 3:-100582388 None:intergenic
GCAGTATCACGTAGCCAACT+TGG 0.457996 3:+100582877 MsG0380017780.01.T01:CDS
CAGAAACGCCCAGTTGCTCT+TGG 0.458612 3:-100582810 None:intergenic
CATTTATATATTGTAAAGGG+TGG 0.467372 3:-100582967 None:intergenic
CACACAACTGATCCTAAATC+AGG 0.475780 3:+100582196 MsG0380017780.01.T01:CDS
AAGTTATTTCCAATTTATCA+AGG 0.477495 3:+100582393 MsG0380017780.01.T01:CDS
ATTCAGCAGCAATCAATCAA+TGG 0.478897 3:+100582147 MsG0380017780.01.T01:exon
CATGGGGCGGGCCAAGGGCA+CGG 0.483921 3:-100582559 None:intergenic
TTAGATTTAGATAGACCATT+CGG 0.484528 3:-100582942 None:intergenic
TTCAGCAGCAATCAATCAAT+GGG 0.491514 3:+100582148 MsG0380017780.01.T01:exon
AAATGATTTCTTCATGGGGC+GGG 0.491715 3:-100582571 None:intergenic
TGCTTTCTGGAGATCAAAGA+AGG 0.491783 3:+100582349 MsG0380017780.01.T01:CDS
ACGGTATCGTCGTATTTGCC+GGG 0.502188 3:-100582540 None:intergenic
ATACGACGATACCGTGCCCT+TGG 0.513413 3:+100582548 MsG0380017780.01.T01:CDS
GGGTTCTCTAATGGCAGGAT+GGG 0.519019 3:+100582168 MsG0380017780.01.T01:CDS
ATACAAAGAAAGAAAGACAT+TGG 0.548215 3:+100582076 MsG0380017780.01.T01:five_prime_UTR
TGGGTTCTCTAATGGCAGGA+TGG 0.562729 3:+100582167 MsG0380017780.01.T01:CDS
ATATTTCAGAATCACTTGAA+AGG 0.563841 3:+100582297 MsG0380017780.01.T01:intron
TCAATGGGTTCTCTAATGGC+AGG 0.564446 3:+100582163 MsG0380017780.01.T01:exon
CATGAAGAAATCATTTGGCA+TGG 0.570197 3:+100582578 MsG0380017780.01.T01:CDS
CAAATGATTTCTTCATGGGG+CGG 0.572003 3:-100582572 None:intergenic
CAGTATCACGTAGCCAACTT+GGG 0.576698 3:+100582878 MsG0380017780.01.T01:CDS
ATACTGCGATGCATACTTGT+TGG 0.576932 3:-100582861 None:intergenic
TCTGAATGAACCTCCAGTGA+TGG 0.578917 3:+100582829 MsG0380017780.01.T01:CDS
GTAGGAGCTTCCATCACTGG+AGG 0.595410 3:-100582839 None:intergenic
GAATTAGTGCTTGTTCCGAA+TGG 0.599911 3:+100582927 MsG0380017780.01.T01:CDS
TGCCAAATGATTTCTTCATG+GGG 0.625447 3:-100582575 None:intergenic
TCAAAATTAAACACAGGACA+TGG 0.627591 3:+100582905 MsG0380017780.01.T01:CDS
TGCGATGCATACTTGTTGGT+AGG 0.629639 3:-100582857 None:intergenic
GAGATCAAAGAAGGAGACAG+AGG 0.641594 3:+100582358 MsG0380017780.01.T01:CDS
GGATCATCAAAATTAAACAC+AGG 0.655378 3:+100582899 MsG0380017780.01.T01:CDS
CAGGTGGACCAAGAGCAACT+GGG 0.675150 3:+100582802 MsG0380017780.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACATTTATATATTGTAAA+GGG - Chr3:100582973-100582992 None:intergenic 10.0%
!! AAAATGTTAATTTATCCATA+AGG + Chr3:100583072-100583091 MsG0380017780.01.T01:three_prime_UTR 15.0%
!! AATTCATTTCGATATTTAAT+TGG + Chr3:100582513-100582532 MsG0380017780.01.T01:intron 15.0%
!! CTTAAAAATATGAAGAAAAA+TGG + Chr3:100582624-100582643 MsG0380017780.01.T01:CDS 15.0%
!! CTTTATATTGAATTTGAATA+TGG + Chr3:100582763-100582782 MsG0380017780.01.T01:intron 15.0%
!! GTTACATTTATATATTGTAA+AGG - Chr3:100582974-100582993 None:intergenic 15.0%
!! ATAAATTGGAAATAACTTTG+AGG - Chr3:100582391-100582410 None:intergenic 20.0%
!! ATTAATTAAGTACCTGATTT+AGG - Chr3:100582211-100582230 None:intergenic 20.0%
!!! AAGTTATTTCCAATTTATCA+AGG + Chr3:100582393-100582412 MsG0380017780.01.T01:CDS 20.0%
!!! TGTCATGAATACTTTTAATT+TGG + Chr3:100582731-100582750 MsG0380017780.01.T01:intron 20.0%
!!! TTAAAAGTATTCATGACAAT+TGG - Chr3:100582729-100582748 None:intergenic 20.0%
!!! TTAATTTATCCATAAGGTTT+TGG + Chr3:100583078-100583097 MsG0380017780.01.T01:three_prime_UTR 20.0%
! AAAATATGAAGAAAAATGGC+TGG + Chr3:100582628-100582647 MsG0380017780.01.T01:CDS 25.0%
! AATTTGAATATGGTTGCATT+GGG + Chr3:100582773-100582792 MsG0380017780.01.T01:intron 25.0%
! ATACAAAGAAAGAAAGACAT+TGG + Chr3:100582076-100582095 MsG0380017780.01.T01:five_prime_UTR 25.0%
! ATATTTCAGAATCACTTGAA+AGG + Chr3:100582297-100582316 MsG0380017780.01.T01:intron 25.0%
! CAAAACAAAAATTACATTCG+TGG - Chr3:100583001-100583020 None:intergenic 25.0%
! CATTTATATATTGTAAAGGG+TGG - Chr3:100582970-100582989 None:intergenic 25.0%
! TTAGATTTAGATAGACCATT+CGG - Chr3:100582945-100582964 None:intergenic 25.0%
AAGAAGAAATTGATGCTTTC+TGG + Chr3:100582336-100582355 MsG0380017780.01.T01:CDS 30.0%
ATGCCAAATGATTTCTTCAT+GGG - Chr3:100582579-100582598 None:intergenic 30.0%
CAATTTATCAAGGACTATTC+AGG + Chr3:100582403-100582422 MsG0380017780.01.T01:CDS 30.0%
CATGGATGTTGACAAAAATA+TGG + Chr3:100582596-100582615 MsG0380017780.01.T01:CDS 30.0%
CTGAATAGTCCTTGATAAAT+TGG - Chr3:100582405-100582424 None:intergenic 30.0%
GAATTTGAATATGGTTGCAT+TGG + Chr3:100582772-100582791 MsG0380017780.01.T01:intron 30.0%
GGATCATCAAAATTAAACAC+AGG + Chr3:100582899-100582918 MsG0380017780.01.T01:CDS 30.0%
TCAAAATTAAACACAGGACA+TGG + Chr3:100582905-100582924 MsG0380017780.01.T01:CDS 30.0%
! GTTTTCTAACGACTAACAAT+TGG - Chr3:100582669-100582688 None:intergenic 30.0%
! TTGGAATTAAGAGTGTGATT+TGG + Chr3:100582095-100582114 MsG0380017780.01.T01:five_prime_UTR 30.0%
!! GAAAGCATCAATTTCTTCTT+TGG - Chr3:100582336-100582355 None:intergenic 30.0%
!! TAATTTTGATGATCCCAAGT+TGG - Chr3:100582894-100582913 None:intergenic 30.0%
AAGTCTGCAAGTAGTTGAAT+TGG - Chr3:100582703-100582722 None:intergenic 35.0%
ATTCAACTACTTGCAGACTT+AGG + Chr3:100582703-100582722 MsG0380017780.01.T01:intron 35.0%
ATTCAGCAGCAATCAATCAA+TGG + Chr3:100582147-100582166 MsG0380017780.01.T01:exon 35.0%
CAGAATCACTTGAAAGGAAT+CGG + Chr3:100582303-100582322 MsG0380017780.01.T01:intron 35.0%
CATGAAGAAATCATTTGGCA+TGG + Chr3:100582578-100582597 MsG0380017780.01.T01:CDS 35.0%
CATGCCAAATGATTTCTTCA+TGG - Chr3:100582580-100582599 None:intergenic 35.0%
TGCCAAATGATTTCTTCATG+GGG - Chr3:100582578-100582597 None:intergenic 35.0%
TTCAGCAGCAATCAATCAAT+GGG + Chr3:100582148-100582167 MsG0380017780.01.T01:exon 35.0%
TTGTCTTTATGCTCTGTGTT+TGG + Chr3:100582254-100582273 MsG0380017780.01.T01:intron 35.0%
! ATGGCTGGTAAATTTTCTCA+TGG + Chr3:100582643-100582662 MsG0380017780.01.T01:intron 35.0%
! TCAATCAATGGGTTCTCTAA+TGG + Chr3:100582159-100582178 MsG0380017780.01.T01:exon 35.0%
AAATGATTTCTTCATGGGGC+GGG - Chr3:100582574-100582593 None:intergenic 40.0%
ATACTGCGATGCATACTTGT+TGG - Chr3:100582864-100582883 None:intergenic 40.0%
CAAATGATTTCTTCATGGGG+CGG - Chr3:100582575-100582594 None:intergenic 40.0%
CACACAACTGATCCTAAATC+AGG + Chr3:100582196-100582215 MsG0380017780.01.T01:CDS 40.0%
CGATATTTAATTGGACAGCC+CGG + Chr3:100582522-100582541 MsG0380017780.01.T01:intron 40.0%
GAATTAGTGCTTGTTCCGAA+TGG + Chr3:100582927-100582946 MsG0380017780.01.T01:CDS 40.0%
! TGCTTTCTGGAGATCAAAGA+AGG + Chr3:100582349-100582368 MsG0380017780.01.T01:CDS 40.0%
CAGTATCACGTAGCCAACTT+GGG + Chr3:100582878-100582897 MsG0380017780.01.T01:CDS 45.0%
CGCCCCATGAAGAAATCATT+TGG + Chr3:100582573-100582592 MsG0380017780.01.T01:CDS 45.0%
GAGATCAAAGAAGGAGACAG+AGG + Chr3:100582358-100582377 MsG0380017780.01.T01:CDS 45.0%
TCTGAATGAACCTCCAGTGA+TGG + Chr3:100582829-100582848 MsG0380017780.01.T01:CDS 45.0%
! ATGCTCTGTGTTTGGCTTCA+CGG + Chr3:100582262-100582281 MsG0380017780.01.T01:intron 45.0%
! TCAATGGGTTCTCTAATGGC+AGG + Chr3:100582163-100582182 MsG0380017780.01.T01:exon 45.0%
! TGCGATGCATACTTGTTGGT+AGG - Chr3:100582860-100582879 None:intergenic 45.0%
ACGGTATCGTCGTATTTGCC+GGG - Chr3:100582543-100582562 None:intergenic 50.0%
CACGGTATCGTCGTATTTGC+CGG - Chr3:100582544-100582563 None:intergenic 50.0%
GCAGTATCACGTAGCCAACT+TGG + Chr3:100582877-100582896 MsG0380017780.01.T01:CDS 50.0%
GGGTTCTCTAATGGCAGGAT+GGG + Chr3:100582168-100582187 MsG0380017780.01.T01:CDS 50.0%
TGGGTTCTCTAATGGCAGGA+TGG + Chr3:100582167-100582186 MsG0380017780.01.T01:CDS 50.0%
TTGGTAGGAGCTTCCATCAC+TGG - Chr3:100582845-100582864 None:intergenic 50.0%
ATACGACGATACCGTGCCCT+TGG + Chr3:100582548-100582567 MsG0380017780.01.T01:CDS 55.0%
CAGAAACGCCCAGTTGCTCT+TGG - Chr3:100582813-100582832 None:intergenic 55.0%
CAGGTGGACCAAGAGCAACT+GGG + Chr3:100582802-100582821 MsG0380017780.01.T01:intron 55.0%
GTAGGAGCTTCCATCACTGG+AGG - Chr3:100582842-100582861 None:intergenic 55.0%
TGGTTGCATTGGGTGAGTGC+AGG + Chr3:100582783-100582802 MsG0380017780.01.T01:intron 55.0%
TTGCATTGGGTGAGTGCAGG+TGG + Chr3:100582786-100582805 MsG0380017780.01.T01:intron 55.0%
GCAGGTGGACCAAGAGCAAC+TGG + Chr3:100582801-100582820 MsG0380017780.01.T01:intron 60.0%
TTCTTCATGGGGCGGGCCAA+GGG - Chr3:100582567-100582586 None:intergenic 60.0%
TTTCTTCATGGGGCGGGCCA+AGG - Chr3:100582568-100582587 None:intergenic 60.0%
CATGGGGCGGGCCAAGGGCA+CGG - Chr3:100582562-100582581 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 100582053 100583100 100582053 ID=MsG0380017780.01;Name=MsG0380017780.01
Chr3 mRNA 100582053 100583100 100582053 ID=MsG0380017780.01.T01;Parent=MsG0380017780.01;Name=MsG0380017780.01.T01;_AED=0.35;_eAED=0.35;_QI=113|1|1|1|0.75|0.6|5|122|144
Chr3 exon 100582053 100582217 100582053 ID=MsG0380017780.01.T01:exon:2322;Parent=MsG0380017780.01.T01
Chr3 exon 100582306 100582424 100582306 ID=MsG0380017780.01.T01:exon:2323;Parent=MsG0380017780.01.T01
Chr3 exon 100582540 100582649 100582540 ID=MsG0380017780.01.T01:exon:2324;Parent=MsG0380017780.01.T01
Chr3 exon 100582805 100583017 100582805 ID=MsG0380017780.01.T01:exon:2325;Parent=MsG0380017780.01.T01
Chr3 exon 100583038 100583100 100583038 ID=MsG0380017780.01.T01:exon:2326;Parent=MsG0380017780.01.T01
Chr3 five_prime_UTR 100582053 100582165 100582053 ID=MsG0380017780.01.T01:five_prime_utr;Parent=MsG0380017780.01.T01
Chr3 CDS 100582166 100582217 100582166 ID=MsG0380017780.01.T01:cds;Parent=MsG0380017780.01.T01
Chr3 CDS 100582306 100582424 100582306 ID=MsG0380017780.01.T01:cds;Parent=MsG0380017780.01.T01
Chr3 CDS 100582540 100582649 100582540 ID=MsG0380017780.01.T01:cds;Parent=MsG0380017780.01.T01
Chr3 CDS 100582805 100582958 100582805 ID=MsG0380017780.01.T01:cds;Parent=MsG0380017780.01.T01
Chr3 three_prime_UTR 100582959 100583017 100582959 ID=MsG0380017780.01.T01:three_prime_utr;Parent=MsG0380017780.01.T01
Chr3 three_prime_UTR 100583038 100583100 100583038 ID=MsG0380017780.01.T01:three_prime_utr;Parent=MsG0380017780.01.T01
Gene Sequence

>MsG0380017780.01.T01

ATGGGTTCTCTAATGGCAGGATGGGACTCTCACACAACTGATCCTAAATCAGAATCACTTGAAAGGAATCGGTCACTCACCAAAGAAGAAATTGATGCTTTCTGGAGATCAAAGAAGGAGACAGAGGATGAACACCTCAAAGTTATTTCCAATTTATCAAGGACTATTCAGCCCGGCAAATACGACGATACCGTGCCCTTGGCCCGCCCCATGAAGAAATCATTTGGCATGGATGTTGACAAAAATATGGAGCAGCTTAAAAATATGAAGAAAAATGGCTGGTGGACCAAGAGCAACTGGGCGTTTCTGAATGAACCTCCAGTGATGGAAGCTCCTACCAACAAGTATGCATCGCAGTATCACGTAGCCAACTTGGGATCATCAAAATTAAACACAGGACATGGAATTAGTGCTTGTTCCGAATGGTCTATCTAA

Protein sequence

>MsG0380017780.01.T01

MGSLMAGWDSHTTDPKSESLERNRSLTKEEIDAFWRSKKETEDEHLKVISNLSRTIQPGKYDDTVPLARPMKKSFGMDVDKNMEQLKNMKKNGWWTKSNWAFLNEPPVMEAPTNKYASQYHVANLGSSKLNTGHGISACSEWSI*