Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036203.01.T01 | CAA7045307.1 | 72.519 | 131 | 36 | 0 | 3 | 133 | 182 | 312 | 8.87E-64 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036203.01.T01 | P04146 | 34.646 | 127 | 76 | 3 | 3 | 129 | 1283 | 1402 | 9.98E-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036203.01.T01 | A0A6D2JWG4 | 72.519 | 131 | 36 | 0 | 3 | 133 | 182 | 312 | 4.24e-64 | 207 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047925.01 | MsG0780036203.01 | 0.807983 | 3.872622e-50 | 2.187825e-47 |
| MsG0080048431.01 | MsG0780036203.01 | 0.813515 | 2.461147e-51 | 1.610634e-48 |
| MsG0380013883.01 | MsG0780036203.01 | 0.807343 | 5.294880e-50 | 2.941878e-47 |
| MsG0480018664.01 | MsG0780036203.01 | 0.811804 | 5.829286e-51 | 3.643330e-48 |
| MsG0580026434.01 | MsG0780036203.01 | 0.802540 | 5.345348e-49 | 2.625238e-46 |
| MsG0280007331.01 | MsG0780036203.01 | 0.811824 | 5.771751e-51 | 3.609322e-48 |
| MsG0280009248.01 | MsG0780036203.01 | 0.825797 | 3.852905e-54 | 3.548197e-51 |
| MsG0780036201.01 | MsG0780036203.01 | 0.807556 | 4.771480e-50 | 2.665720e-47 |
| MsG0780036203.01 | MsG0880044644.01 | 0.805751 | 1.147508e-49 | 6.120410e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCAAGCAAAGAGTGTAGA+TGG | 0.431701 | 7:-4638725 | MsG0780036203.01.T01:CDS |
| TTGTCTTCATACAAGATTGT+TGG | 0.433179 | 7:+4638645 | None:intergenic |
| AAGAAGCACATACGAAGTAT+TGG | 0.444398 | 7:-4638438 | MsG0780036203.01.T01:CDS |
| GAACTTAGGAGGGATATGTT+TGG | 0.468173 | 7:+4638572 | None:intergenic |
| TGAGTGAATGAGAAGAACTT+AGG | 0.478194 | 7:+4638558 | None:intergenic |
| TACTTCAGCATGATTTGAAG+AGG | 0.525244 | 7:+4638761 | None:intergenic |
| TCCTTGGATATGTTGCAAAA+CGG | 0.528114 | 7:+4638695 | None:intergenic |
| TGTGTTACTCAAATGAAAGA+AGG | 0.534472 | 7:-4638615 | MsG0780036203.01.T01:CDS |
| GTGAATGAGAAGAACTTAGG+AGG | 0.536327 | 7:+4638561 | None:intergenic |
| TGAATGAGAAGAACTTAGGA+GGG | 0.539803 | 7:+4638562 | None:intergenic |
| TGCTTCTTGAAAACTGATGT+AGG | 0.544161 | 7:+4638453 | None:intergenic |
| TTGTTAAACAGACCACTCCT+TGG | 0.560252 | 7:+4638679 | None:intergenic |
| TAGGGAGTGCCTTTGTGAAG+AGG | 0.583440 | 7:+4638472 | None:intergenic |
| GCTTCTTGAAAACTGATGTA+GGG | 0.606058 | 7:+4638454 | None:intergenic |
| TTTGCAACATATCCAAGGAG+TGG | 0.636844 | 7:-4638691 | MsG0780036203.01.T01:CDS |
| TATCTGTTCGAGTGATAATG+TGG | 0.637069 | 7:-4638499 | MsG0780036203.01.T01:CDS |
| TGTGGCAGACCTCTTCACAA+AGG | 0.728433 | 7:-4638481 | MsG0780036203.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGTCTTCATACAAGATTGT+TGG | + | Chr7:4638563-4638582 | None:intergenic | 30.0% | |
| ! | TGTGTTACTCAAATGAAAGA+AGG | - | Chr7:4638590-4638609 | MsG0780036203.01.T01:CDS | 30.0% |
| GCTTCTTGAAAACTGATGTA+GGG | + | Chr7:4638754-4638773 | None:intergenic | 35.0% | |
| TACTTCAGCATGATTTGAAG+AGG | + | Chr7:4638447-4638466 | None:intergenic | 35.0% | |
| TCCTTGGATATGTTGCAAAA+CGG | + | Chr7:4638513-4638532 | None:intergenic | 35.0% | |
| TGAATGAGAAGAACTTAGGA+GGG | + | Chr7:4638646-4638665 | None:intergenic | 35.0% | |
| TGAGTGAATGAGAAGAACTT+AGG | + | Chr7:4638650-4638669 | None:intergenic | 35.0% | |
| TGCTTCTTGAAAACTGATGT+AGG | + | Chr7:4638755-4638774 | None:intergenic | 35.0% | |
| ! | AAGAAGCACATACGAAGTAT+TGG | - | Chr7:4638767-4638786 | MsG0780036203.01.T01:CDS | 35.0% |
| ! | TATCTGTTCGAGTGATAATG+TGG | - | Chr7:4638706-4638725 | MsG0780036203.01.T01:CDS | 35.0% |
| GAACTTAGGAGGGATATGTT+TGG | + | Chr7:4638636-4638655 | None:intergenic | 40.0% | |
| GTGAATGAGAAGAACTTAGG+AGG | + | Chr7:4638647-4638666 | None:intergenic | 40.0% | |
| TTGTTAAACAGACCACTCCT+TGG | + | Chr7:4638529-4638548 | None:intergenic | 40.0% | |
| TTTGCAACATATCCAAGGAG+TGG | - | Chr7:4638514-4638533 | MsG0780036203.01.T01:CDS | 40.0% | |
| ! | AAGCAAGCAAAGAGTGTAGA+TGG | - | Chr7:4638480-4638499 | MsG0780036203.01.T01:CDS | 40.0% |
| ! | TCCGTTTTGCAACATATCCA+AGG | - | Chr7:4638509-4638528 | MsG0780036203.01.T01:CDS | 40.0% |
| TGTGGCAGACCTCTTCACAA+AGG | - | Chr7:4638724-4638743 | MsG0780036203.01.T01:CDS | 50.0% | |
| !! | TAGGGAGTGCCTTTGTGAAG+AGG | + | Chr7:4638736-4638755 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 4638413 | 4638814 | 4638413 | ID=MsG0780036203.01;Name=MsG0780036203.01 |
| Chr7 | mRNA | 4638413 | 4638814 | 4638413 | ID=MsG0780036203.01.T01;Parent=MsG0780036203.01;Name=MsG0780036203.01.T01;_AED=0.48;_eAED=0.86;_QI=0|-1|0|1|-1|1|1|0|133 |
| Chr7 | exon | 4638413 | 4638814 | 4638413 | ID=MsG0780036203.01.T01:exon:2617;Parent=MsG0780036203.01.T01 |
| Chr7 | CDS | 4638413 | 4638814 | 4638413 | ID=MsG0780036203.01.T01:cds;Parent=MsG0780036203.01.T01 |
| Gene Sequence |
| Protein sequence |
