Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026434.01.T01 | XP_003589014.1 | 91.615 | 322 | 26 | 1 | 1 | 322 | 1 | 321 | 0 | 602 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026434.01.T01 | Q9FFJ8 | 24.29 | 317 | 215 | 6 | 6 | 322 | 156 | 447 | 4.66E-20 | 93.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026434.01.T01 | G7IB81 | 91.615 | 322 | 26 | 1 | 1 | 322 | 1 | 321 | 0.0 | 602 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002233.01 | MsG0580026434.01 | 0.800689 | 1.281122e-48 | 6.003255e-46 |
| MsG0180002234.01 | MsG0580026434.01 | 0.813018 | 3.164386e-51 | 2.043020e-48 |
| MsG0180002518.01 | MsG0580026434.01 | 0.830371 | 3.049005e-55 | 3.206292e-52 |
| MsG0280006325.01 | MsG0580026434.01 | 0.811107 | 8.258421e-51 | 5.067645e-48 |
| MsG0380011786.01 | MsG0580026434.01 | 0.807610 | 4.647391e-50 | 2.600017e-47 |
| MsG0380013883.01 | MsG0580026434.01 | 0.833339 | 5.644591e-56 | 6.481931e-53 |
| MsG0380014422.01 | MsG0580026434.01 | 0.819713 | 1.004958e-52 | 7.793199e-50 |
| MsG0380014867.01 | MsG0580026434.01 | 0.815176 | 1.057361e-51 | 7.237138e-49 |
| MsG0480018664.01 | MsG0580026434.01 | 0.814124 | 1.807663e-51 | 1.202618e-48 |
| MsG0480019482.01 | MsG0580026434.01 | 0.803587 | 3.247258e-49 | 1.638146e-46 |
| MsG0480021587.01 | MsG0580026434.01 | 0.823904 | 1.077297e-53 | 9.399177e-51 |
| MsG0380014977.01 | MsG0580026434.01 | 0.841659 | 4.156194e-58 | 6.148716e-55 |
| MsG0380017312.01 | MsG0580026434.01 | 0.803905 | 2.788469e-49 | 1.418278e-46 |
| MsG0380017820.01 | MsG0580026434.01 | 0.812640 | 3.829239e-51 | 2.447730e-48 |
| MsG0580026222.01 | MsG0580026434.01 | 0.862886 | 3.688685e-64 | 1.097338e-60 |
| MsG0580026276.01 | MsG0580026434.01 | 0.821787 | 3.352815e-53 | 2.754767e-50 |
| MsG0580026303.01 | MsG0580026434.01 | 0.817380 | 3.397706e-52 | 2.470580e-49 |
| MsG0580026434.01 | MsG0580026631.01 | 0.812189 | 4.804467e-51 | 3.034399e-48 |
| MsG0580026434.01 | MsG0580027332.01 | 0.829659 | 4.549059e-55 | 4.686168e-52 |
| MsG0580026434.01 | MsG0580027970.01 | 0.802452 | 5.573155e-49 | 2.731013e-46 |
| MsG0580026434.01 | MsG0580028169.01 | 0.810323 | 1.220369e-50 | 7.333760e-48 |
| MsG0580026434.01 | MsG0580028170.01 | 0.815103 | 1.097124e-51 | 7.494555e-49 |
| MsG0580026434.01 | MsG0580029381.01 | 0.865220 | 6.915521e-65 | 2.232662e-61 |
| MsG0580026434.01 | MsG0680031101.01 | 0.801817 | 7.527119e-49 | 3.629385e-46 |
| MsG0580026434.01 | MsG0680031380.01 | 0.829853 | 4.079638e-55 | 4.227002e-52 |
| MsG0580026434.01 | MsG0680033050.01 | 0.811861 | 5.664226e-51 | 3.545592e-48 |
| MsG0580026434.01 | MsG0680035896.01 | 0.809683 | 1.676361e-50 | 9.906944e-48 |
| MsG0580026434.01 | MsG0780036134.01 | 0.801084 | 1.063692e-48 | 5.034980e-46 |
| MsG0580026434.01 | MsG0780036203.01 | 0.802540 | 5.345348e-49 | 2.625238e-46 |
| MsG0580026434.01 | MsG0780036498.01 | 0.802683 | 4.993115e-49 | 2.461187e-46 |
| MsG0580026434.01 | MsG0780037606.01 | 0.826690 | 2.361638e-54 | 2.230741e-51 |
| MsG0580026434.01 | MsG0780039282.01 | 0.845299 | 4.430024e-59 | 7.349777e-56 |
| MsG0580026434.01 | MsG0880044644.01 | 0.818187 | 2.233691e-52 | 1.660499e-49 |
| MsG0280006551.01 | MsG0580026434.01 | 0.844050 | 9.609505e-59 | 1.532842e-55 |
| MsG0280008759.01 | MsG0580026434.01 | 0.821813 | 3.306767e-53 | 2.719023e-50 |
| MsG0280009248.01 | MsG0580026434.01 | 0.832344 | 9.976100e-56 | 1.111830e-52 |
| MsG0280009306.01 | MsG0580026434.01 | 0.824321 | 8.599560e-54 | 7.591140e-51 |
| MsG0280009371.01 | MsG0580026434.01 | 0.828061 | 1.108138e-54 | 1.089455e-51 |
| MsG0280010117.01 | MsG0580026434.01 | 0.807734 | 4.373763e-50 | 2.454994e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026434.01.T01 | MTR_1g016370 | 91.615 | 322 | 26 | 1 | 1 | 322 | 1 | 321 | 0.0 | 602 |
| MsG0580026434.01.T01 | MTR_4g061370 | 87.888 | 322 | 20 | 2 | 1 | 322 | 1013 | 1315 | 0.0 | 550 |
| MsG0580026434.01.T01 | MTR_5g055390 | 76.797 | 306 | 27 | 2 | 17 | 322 | 20 | 281 | 7.85e-162 | 453 |
| MsG0580026434.01.T01 | MTR_8g054100 | 89.669 | 242 | 21 | 1 | 1 | 242 | 1 | 238 | 1.99e-148 | 417 |
| MsG0580026434.01.T01 | MTR_2g018710 | 63.987 | 311 | 108 | 3 | 13 | 321 | 14 | 322 | 8.82e-141 | 401 |
| MsG0580026434.01.T01 | MTR_2g086350 | 63.987 | 311 | 108 | 3 | 13 | 321 | 14 | 322 | 2.07e-140 | 400 |
| MsG0580026434.01.T01 | MTR_1g080080 | 63.987 | 311 | 108 | 3 | 13 | 321 | 14 | 322 | 2.63e-140 | 400 |
| MsG0580026434.01.T01 | MTR_3g110155 | 57.057 | 333 | 126 | 4 | 1 | 321 | 1 | 328 | 9.69e-128 | 368 |
| MsG0580026434.01.T01 | MTR_8g006585 | 57.057 | 333 | 126 | 4 | 1 | 321 | 1 | 328 | 9.69e-128 | 368 |
| MsG0580026434.01.T01 | MTR_8g006695 | 57.057 | 333 | 126 | 4 | 1 | 321 | 1 | 328 | 9.69e-128 | 368 |
| MsG0580026434.01.T01 | MTR_5g054570 | 60.450 | 311 | 101 | 4 | 13 | 321 | 14 | 304 | 1.46e-126 | 364 |
| MsG0580026434.01.T01 | MTR_2g435870 | 54.575 | 306 | 114 | 3 | 19 | 321 | 5 | 288 | 4.99e-114 | 332 |
| MsG0580026434.01.T01 | MTR_4g046743 | 52.581 | 310 | 96 | 4 | 13 | 321 | 14 | 273 | 1.82e-101 | 299 |
| MsG0580026434.01.T01 | MTR_2g054730 | 85.915 | 142 | 20 | 0 | 181 | 322 | 11 | 152 | 1.53e-78 | 236 |
| MsG0580026434.01.T01 | MTR_2g436180 | 49.123 | 228 | 76 | 4 | 100 | 321 | 1 | 194 | 1.11e-62 | 197 |
| MsG0580026434.01.T01 | MTR_7g029320 | 46.000 | 250 | 76 | 5 | 13 | 261 | 14 | 205 | 1.09e-59 | 190 |
| MsG0580026434.01.T01 | MTR_3g023200 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 8.96e-45 | 148 |
| MsG0580026434.01.T01 | MTR_2g436540 | 41.410 | 227 | 103 | 6 | 119 | 321 | 10 | 230 | 1.06e-44 | 153 |
| MsG0580026434.01.T01 | MTR_3g448480 | 51.974 | 152 | 43 | 3 | 13 | 163 | 14 | 136 | 2.81e-40 | 138 |
| MsG0580026434.01.T01 | MTR_0961s0020 | 69.767 | 86 | 23 | 2 | 122 | 206 | 5 | 88 | 4.61e-31 | 113 |
| MsG0580026434.01.T01 | MTR_1g073440 | 38.028 | 142 | 87 | 1 | 21 | 161 | 15 | 156 | 4.50e-29 | 114 |
| MsG0580026434.01.T01 | MTR_8g105430 | 36.667 | 150 | 94 | 1 | 12 | 161 | 31 | 179 | 6.04e-27 | 106 |
| MsG0580026434.01.T01 | MTR_4g043620 | 38.636 | 132 | 81 | 0 | 30 | 161 | 25 | 156 | 2.04e-26 | 106 |
| MsG0580026434.01.T01 | MTR_8g468830 | 27.619 | 315 | 199 | 8 | 21 | 319 | 14 | 315 | 2.41e-25 | 103 |
| MsG0580026434.01.T01 | MTR_5g018260 | 27.937 | 315 | 199 | 9 | 22 | 315 | 16 | 323 | 1.23e-24 | 102 |
| MsG0580026434.01.T01 | MTR_8g069895 | 34.848 | 132 | 86 | 0 | 30 | 161 | 25 | 156 | 2.28e-23 | 98.2 |
| MsG0580026434.01.T01 | MTR_3g070080 | 28.477 | 302 | 168 | 9 | 12 | 311 | 373 | 628 | 1.82e-22 | 98.6 |
| MsG0580026434.01.T01 | MTR_3g070080 | 38.931 | 131 | 79 | 1 | 22 | 152 | 185 | 314 | 3.57e-22 | 97.4 |
| MsG0580026434.01.T01 | MTR_3g070080 | 34.677 | 124 | 80 | 1 | 21 | 144 | 7 | 129 | 3.29e-15 | 76.6 |
| MsG0580026434.01.T01 | MTR_3g070080 | 28.477 | 302 | 168 | 9 | 12 | 311 | 436 | 691 | 2.12e-22 | 98.2 |
| MsG0580026434.01.T01 | MTR_3g070080 | 38.931 | 131 | 79 | 1 | 22 | 152 | 248 | 377 | 3.14e-22 | 97.8 |
| MsG0580026434.01.T01 | MTR_3g070080 | 34.677 | 124 | 80 | 1 | 21 | 144 | 70 | 192 | 2.69e-15 | 77.0 |
| MsG0580026434.01.T01 | MTR_5g022570 | 28.667 | 300 | 182 | 8 | 19 | 312 | 17 | 290 | 3.86e-22 | 94.7 |
| MsG0580026434.01.T01 | MTR_7g056530 | 63.934 | 61 | 22 | 0 | 103 | 163 | 28 | 88 | 6.09e-21 | 86.7 |
| MsG0580026434.01.T01 | MTR_2g021280 | 33.333 | 132 | 88 | 0 | 30 | 161 | 25 | 156 | 2.41e-20 | 90.9 |
| MsG0580026434.01.T01 | MTR_0693s0050 | 41.007 | 139 | 70 | 5 | 27 | 158 | 14 | 147 | 1.38e-19 | 87.0 |
| MsG0580026434.01.T01 | MTR_4g045750 | 58.750 | 80 | 30 | 2 | 139 | 217 | 61 | 138 | 1.38e-19 | 84.0 |
| MsG0580026434.01.T01 | MTR_7g034425 | 53.247 | 77 | 35 | 1 | 12 | 87 | 85 | 161 | 4.73e-19 | 83.2 |
| MsG0580026434.01.T01 | MTR_4g029080 | 41.975 | 81 | 47 | 0 | 81 | 161 | 2 | 82 | 8.70e-15 | 69.7 |
| MsG0580026434.01.T01 | MTR_8g446440 | 25.490 | 255 | 151 | 10 | 81 | 319 | 2 | 233 | 3.44e-14 | 71.2 |
| MsG0580026434.01.T01 | MTR_7g105890 | 40.566 | 106 | 52 | 4 | 27 | 127 | 14 | 113 | 3.75e-14 | 72.8 |
| MsG0580026434.01.T01 | MTR_4g128540 | 39.623 | 106 | 53 | 4 | 27 | 127 | 14 | 113 | 1.51e-13 | 70.9 |
| MsG0580026434.01.T01 | MTR_4g128327 | 39.796 | 98 | 49 | 3 | 27 | 119 | 14 | 106 | 4.17e-13 | 65.9 |
| MsG0580026434.01.T01 | MTR_3g053640 | 24.918 | 305 | 177 | 11 | 13 | 310 | 171 | 430 | 8.62e-12 | 65.9 |
| MsG0580026434.01.T01 | MTR_1g055365 | 28.346 | 127 | 90 | 1 | 20 | 146 | 191 | 316 | 1.44e-11 | 65.9 |
| MsG0580026434.01.T01 | MTR_1g055365 | 26.471 | 136 | 99 | 1 | 21 | 156 | 21 | 155 | 4.42e-11 | 64.3 |
| MsG0580026434.01.T01 | MTR_8g085310 | 23.569 | 297 | 192 | 9 | 25 | 310 | 434 | 706 | 8.51e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026434.01.T01 | AT5G05800 | 24.290 | 317 | 215 | 6 | 6 | 322 | 156 | 447 | 4.76e-21 | 93.6 |
| MsG0580026434.01.T01 | AT5G05800 | 34.234 | 111 | 72 | 1 | 20 | 130 | 2 | 111 | 2.35e-15 | 77.0 |
| MsG0580026434.01.T01 | AT5G05800 | 24.290 | 317 | 215 | 6 | 6 | 322 | 156 | 447 | 4.76e-21 | 93.6 |
| MsG0580026434.01.T01 | AT5G05800 | 34.234 | 111 | 72 | 1 | 20 | 130 | 2 | 111 | 2.35e-15 | 77.0 |
| MsG0580026434.01.T01 | AT5G05800 | 24.290 | 317 | 215 | 6 | 6 | 322 | 156 | 447 | 4.76e-21 | 93.6 |
| MsG0580026434.01.T01 | AT5G05800 | 34.234 | 111 | 72 | 1 | 20 | 130 | 2 | 111 | 2.35e-15 | 77.0 |
| MsG0580026434.01.T01 | AT5G05800 | 24.290 | 317 | 215 | 6 | 6 | 322 | 156 | 447 | 4.76e-21 | 93.6 |
| MsG0580026434.01.T01 | AT5G05800 | 34.234 | 111 | 72 | 1 | 20 | 130 | 2 | 111 | 2.35e-15 | 77.0 |
| MsG0580026434.01.T01 | AT5G05800 | 24.290 | 317 | 215 | 6 | 6 | 322 | 156 | 447 | 4.76e-21 | 93.6 |
| MsG0580026434.01.T01 | AT5G05800 | 34.234 | 111 | 72 | 1 | 20 | 130 | 2 | 111 | 2.35e-15 | 77.0 |
| MsG0580026434.01.T01 | AT3G11290 | 26.144 | 306 | 207 | 7 | 12 | 310 | 155 | 448 | 4.40e-16 | 79.0 |
| MsG0580026434.01.T01 | AT3G11290 | 26.144 | 306 | 207 | 7 | 12 | 310 | 155 | 448 | 4.40e-16 | 79.0 |
| MsG0580026434.01.T01 | AT2G24960 | 27.737 | 137 | 98 | 1 | 8 | 144 | 155 | 290 | 2.30e-12 | 68.2 |
| MsG0580026434.01.T01 | AT2G24960 | 27.737 | 137 | 98 | 1 | 8 | 144 | 155 | 290 | 2.71e-12 | 68.2 |
| MsG0580026434.01.T01 | AT2G24960 | 27.273 | 143 | 103 | 1 | 12 | 154 | 314 | 455 | 4.36e-11 | 64.3 |
| MsG0580026434.01.T01 | AT3G11310 | 32.168 | 143 | 91 | 2 | 20 | 157 | 171 | 312 | 4.19e-11 | 64.3 |
Find 65 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCATGGTATAACTTATT+TGG | 0.134716 | 5:-37171965 | MsG0580026434.01.T01:CDS |
| ACAACTGAATTTGCAAATAT+TGG | 0.140047 | 5:-37171562 | MsG0580026434.01.T01:CDS |
| GGCAGAAAACAAAAGCTATT+TGG | 0.232374 | 5:-37172174 | MsG0580026434.01.T01:CDS |
| AACTTATTTGGGAGAGAAAC+TGG | 0.287945 | 5:-37171953 | MsG0580026434.01.T01:CDS |
| CAACTGAATTTGCAAATATT+GGG | 0.308854 | 5:-37171561 | MsG0580026434.01.T01:CDS |
| GAAATAAACATCTCCCTTGC+AGG | 0.323302 | 5:+37171271 | None:intergenic |
| AAACTTTGTGAAAGCATGTT+TGG | 0.334075 | 5:-37172134 | MsG0580026434.01.T01:CDS |
| GCAGAAAACAAAAGCTATTT+GGG | 0.339319 | 5:-37172173 | MsG0580026434.01.T01:CDS |
| TTTAAGGATGTAGTGGCTAA+TGG | 0.351555 | 5:-37171712 | MsG0580026434.01.T01:CDS |
| CGTACCAACACGTTCTCTAT+TGG | 0.354820 | 5:+37172102 | None:intergenic |
| GAGCATGGTATAACTTATTT+GGG | 0.363157 | 5:-37171964 | MsG0580026434.01.T01:CDS |
| TCTATTACATACCAGCTGTT+TGG | 0.363433 | 5:+37171874 | None:intergenic |
| TTTGGGAGAGAAACTGGATT+AGG | 0.364125 | 5:-37171947 | MsG0580026434.01.T01:CDS |
| CAGAGGAGAAGAGGTTGAAT+TGG | 0.373243 | 5:-37171237 | MsG0580026434.01.T01:CDS |
| GTTCGGTTAAATCATGAAAT+CGG | 0.390304 | 5:+37172044 | None:intergenic |
| GGTTCCTCCTCACCTAATGA+TGG | 0.395108 | 5:-37172199 | MsG0580026434.01.T01:CDS |
| TTGGTCTCCCACCACTCATC+AGG | 0.427119 | 5:+37171893 | None:intergenic |
| AGCCTTCTCATACTTCAGTT+CGG | 0.461572 | 5:+37172027 | None:intergenic |
| AGAAGGCTCAACTAAAGAAT+AGG | 0.479515 | 5:-37172012 | MsG0580026434.01.T01:CDS |
| GCTGCTGGTAATGCATCTAT+TGG | 0.486263 | 5:-37171382 | MsG0580026434.01.T01:CDS |
| GGAGAGAAACTGGATTAGGA+TGG | 0.487744 | 5:-37171943 | MsG0580026434.01.T01:CDS |
| GTTCCTCCTCACCTAATGAT+GGG | 0.491567 | 5:-37172198 | MsG0580026434.01.T01:CDS |
| GATGAGGCAGTGGCTGTAGA+AGG | 0.502147 | 5:-37171619 | MsG0580026434.01.T01:CDS |
| GTCAAAAGAGTGGTGAGAAA+AGG | 0.505016 | 5:-37171513 | MsG0580026434.01.T01:CDS |
| TGCTGATGTTTAAGCCTGCA+AGG | 0.517021 | 5:-37171285 | MsG0580026434.01.T01:CDS |
| TTCCTCCTCACCTAATGATG+GGG | 0.519823 | 5:-37172197 | MsG0580026434.01.T01:CDS |
| TGGCTAATGGAGAGTACACT+TGG | 0.522261 | 5:-37171699 | MsG0580026434.01.T01:CDS |
| GCAGTGGCTGTAGAAGGATC+TGG | 0.527670 | 5:-37171613 | MsG0580026434.01.T01:CDS |
| TCCTCCTCACCTAATGATGG+GGG | 0.527940 | 5:-37172196 | MsG0580026434.01.T01:CDS |
| ATGACAGTCTGAGAAAGGAT+TGG | 0.531322 | 5:-37171988 | MsG0580026434.01.T01:CDS |
| CTTCTCCTCTGAACCTCGTA+GGG | 0.535159 | 5:+37171249 | None:intergenic |
| TAGGTATGACAGTCTGAGAA+AGG | 0.543958 | 5:-37171993 | MsG0580026434.01.T01:CDS |
| AGGTTAGTCATGAATGGCAG+TGG | 0.548613 | 5:+37171193 | None:intergenic |
| TCACACAGGTTAGTCATGAA+TGG | 0.548759 | 5:+37171187 | None:intergenic |
| CGTTCTCGCAATGATGCTGC+TGG | 0.555390 | 5:-37171397 | MsG0580026434.01.T01:CDS |
| TATTTCCCTACGAGGTTCAG+AGG | 0.555424 | 5:-37171254 | MsG0580026434.01.T01:CDS |
| ATCATTTGAGACTTCATCCA+TGG | 0.558263 | 5:+37171336 | None:intergenic |
| CATGACTAACCTGTGTGACT+AGG | 0.563491 | 5:-37171182 | None:intergenic |
| TACGAGGTTCAGAGGAGAAG+AGG | 0.567800 | 5:-37171246 | MsG0580026434.01.T01:CDS |
| CATGGCAATACCCCACATGA+TGG | 0.569277 | 5:+37171673 | None:intergenic |
| TACACTTGGGCACCATCATG+TGG | 0.573130 | 5:-37171685 | MsG0580026434.01.T01:CDS |
| GTGGTTAACTCACTGGCGAA+TGG | 0.579267 | 5:+37171739 | None:intergenic |
| TGCATCTATTGGTGAAGTGA+TGG | 0.591393 | 5:-37171371 | MsG0580026434.01.T01:CDS |
| GCTGATGTTTAAGCCTGCAA+GGG | 0.594722 | 5:-37171284 | MsG0580026434.01.T01:CDS |
| TCTTCTCCTCTGAACCTCGT+AGG | 0.595412 | 5:+37171248 | None:intergenic |
| GAGAGAAACTGGATTAGGAT+GGG | 0.602509 | 5:-37171942 | MsG0580026434.01.T01:CDS |
| GATAGTGAGGATGCAAGTGT+TGG | 0.604914 | 5:-37171589 | MsG0580026434.01.T01:CDS |
| TGAGAAAGGATTGGAGAGCA+TGG | 0.612846 | 5:-37171979 | MsG0580026434.01.T01:CDS |
| AGAAGGATCTGGCGATAGTG+AGG | 0.613640 | 5:-37171602 | MsG0580026434.01.T01:CDS |
| GCTACAACAGTGTTGTTCAC+AGG | 0.614063 | 5:+37171917 | None:intergenic |
| CCTCCTCACCTAATGATGGG+GGG | 0.614154 | 5:-37172195 | MsG0580026434.01.T01:CDS |
| AACCGAACTGAAGTATGAGA+AGG | 0.615476 | 5:-37172029 | MsG0580026434.01.T01:CDS |
| GTGGTGGGAGACCAAACAGC+TGG | 0.618177 | 5:-37171885 | MsG0580026434.01.T01:intron |
| ACACTTGGGCACCATCATGT+GGG | 0.626839 | 5:-37171684 | MsG0580026434.01.T01:CDS |
| TTGTAGCACCTGATGAGTGG+TGG | 0.632872 | 5:-37171901 | MsG0580026434.01.T01:CDS |
| GGTTAGTCATGAATGGCAGT+GGG | 0.638001 | 5:+37171194 | None:intergenic |
| CCCATACATCCTAGTCACAC+AGG | 0.638813 | 5:+37171173 | None:intergenic |
| CACTTGGGCACCATCATGTG+GGG | 0.646121 | 5:-37171683 | MsG0580026434.01.T01:CDS |
| TGTAGCACCTGATGAGTGGT+GGG | 0.651018 | 5:-37171900 | MsG0580026434.01.T01:CDS |
| GATGGCTGAGCTTCACACCA+TGG | 0.663371 | 5:-37171353 | MsG0580026434.01.T01:CDS |
| AAAACCGATGGTCAAAAGAG+TGG | 0.667696 | 5:-37171523 | MsG0580026434.01.T01:CDS |
| GTCTCCAATAGAGAACGTGT+TGG | 0.677392 | 5:-37172106 | MsG0580026434.01.T01:CDS |
| GGCTAATGGAGAGTACACTT+GGG | 0.684848 | 5:-37171698 | MsG0580026434.01.T01:CDS |
| CTGTTGTAGCACCTGATGAG+TGG | 0.705267 | 5:-37171904 | MsG0580026434.01.T01:CDS |
| GAGATGTTTATTTCCCTACG+AGG | 0.736446 | 5:-37171262 | MsG0580026434.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGAAAAATTTAGGAACAA+GGG | - | Chr5:37171643-37171662 | MsG0580026434.01.T01:CDS | 20.0% |
| !! | TTATGAAAAATTTAGGAACA+AGG | - | Chr5:37171642-37171661 | MsG0580026434.01.T01:CDS | 20.0% |
| !!! | TCCTAAATTTTTCATAAAGA+GGG | + | Chr5:37171639-37171658 | None:intergenic | 20.0% |
| !!! | TTCCTAAATTTTTCATAAAG+AGG | + | Chr5:37171640-37171659 | None:intergenic | 20.0% |
| ! | AAAAGGGTGGAAAAATATTA+AGG | - | Chr5:37171341-37171360 | MsG0580026434.01.T01:CDS | 25.0% |
| ! | AACACAAGTATACATATCTA+AGG | + | Chr5:37171581-37171600 | None:intergenic | 25.0% |
| ! | AATACCAAGAAAAATGTTGT+GGG | - | Chr5:37171189-37171208 | MsG0580026434.01.T01:CDS | 25.0% |
| ! | ACAACTGAATTTGCAAATAT+TGG | - | Chr5:37171847-37171866 | MsG0580026434.01.T01:intron | 25.0% |
| ! | ACCCTCTTTATGAAAAATTT+AGG | - | Chr5:37171635-37171654 | MsG0580026434.01.T01:CDS | 25.0% |
| ! | ATGAAAAATTTAGGAACAAG+GGG | - | Chr5:37171644-37171663 | MsG0580026434.01.T01:CDS | 25.0% |
| ! | CAACTGAATTTGCAAATATT+GGG | - | Chr5:37171848-37171867 | MsG0580026434.01.T01:intron | 25.0% |
| !!! | ATGATCTTTATTTTCATGCT+CGG | - | Chr5:37172091-37172110 | MsG0580026434.01.T01:CDS | 25.0% |
| AATTCTTCACAAAAAACCGA+TGG | - | Chr5:37171874-37171893 | MsG0580026434.01.T01:intron | 30.0% | |
| AGAGCATGGTATAACTTATT+TGG | - | Chr5:37171444-37171463 | MsG0580026434.01.T01:CDS | 30.0% | |
| GAATACCAAGAAAAATGTTG+TGG | - | Chr5:37171188-37171207 | MsG0580026434.01.T01:CDS | 30.0% | |
| GAGCATGGTATAACTTATTT+GGG | - | Chr5:37171445-37171464 | MsG0580026434.01.T01:CDS | 30.0% | |
| GCAGAAAACAAAAGCTATTT+GGG | - | Chr5:37171236-37171255 | MsG0580026434.01.T01:CDS | 30.0% | |
| GTTCGGTTAAATCATGAAAT+CGG | + | Chr5:37171368-37171387 | None:intergenic | 30.0% | |
| ! | AAACTTTGTGAAAGCATGTT+TGG | - | Chr5:37171275-37171294 | MsG0580026434.01.T01:CDS | 30.0% |
| ! | GTACGAGCTTTTCAAAAAAA+GGG | - | Chr5:37171325-37171344 | MsG0580026434.01.T01:CDS | 30.0% |
| ! | TCATCATTTTCAAACACACA+TGG | + | Chr5:37171757-37171776 | None:intergenic | 30.0% |
| ! | TGATAATGATGATGATGATG+AGG | - | Chr5:37171774-37171793 | MsG0580026434.01.T01:CDS | 30.0% |
| !! | TGAGTTAACCACACTTTTTA+AGG | - | Chr5:37171681-37171700 | MsG0580026434.01.T01:CDS | 30.0% |
| AACTTATTTGGGAGAGAAAC+TGG | - | Chr5:37171456-37171475 | MsG0580026434.01.T01:CDS | 35.0% | |
| AGAAGGCTCAACTAAAGAAT+AGG | - | Chr5:37171397-37171416 | MsG0580026434.01.T01:CDS | 35.0% | |
| CACTACATCCTTAAAAAGTG+TGG | + | Chr5:37171692-37171711 | None:intergenic | 35.0% | |
| GATGTGAGTTATGAAAATGC+AGG | - | Chr5:37171609-37171628 | MsG0580026434.01.T01:CDS | 35.0% | |
| GGCAGAAAACAAAAGCTATT+TGG | - | Chr5:37171235-37171254 | MsG0580026434.01.T01:CDS | 35.0% | |
| TCTATTACATACCAGCTGTT+TGG | + | Chr5:37171538-37171557 | None:intergenic | 35.0% | |
| TTTAAGGATGTAGTGGCTAA+TGG | - | Chr5:37171697-37171716 | MsG0580026434.01.T01:CDS | 35.0% | |
| ! | AAAAAGTGTGGTTAACTCAC+TGG | + | Chr5:37171680-37171699 | None:intergenic | 35.0% |
| ! | ATCATTTGAGACTTCATCCA+TGG | + | Chr5:37172076-37172095 | None:intergenic | 35.0% |
| ! | GGTACGAGCTTTTCAAAAAA+AGG | - | Chr5:37171324-37171343 | MsG0580026434.01.T01:CDS | 35.0% |
| !! | CACACTTTTTAAGGATGTAG+TGG | - | Chr5:37171690-37171709 | MsG0580026434.01.T01:CDS | 35.0% |
| !!! | GCACTTGCTTTTTTCTTACT+TGG | + | Chr5:37171946-37171965 | None:intergenic | 35.0% |
| AAAACCGATGGTCAAAAGAG+TGG | - | Chr5:37171886-37171905 | MsG0580026434.01.T01:CDS | 40.0% | |
| AACCGAACTGAAGTATGAGA+AGG | - | Chr5:37171380-37171399 | MsG0580026434.01.T01:CDS | 40.0% | |
| AGCCTTCTCATACTTCAGTT+CGG | + | Chr5:37171385-37171404 | None:intergenic | 40.0% | |
| ATGACAGTCTGAGAAAGGAT+TGG | - | Chr5:37171421-37171440 | MsG0580026434.01.T01:CDS | 40.0% | |
| GAAATAAACATCTCCCTTGC+AGG | + | Chr5:37172141-37172160 | None:intergenic | 40.0% | |
| GAGATGTTTATTTCCCTACG+AGG | - | Chr5:37172147-37172166 | MsG0580026434.01.T01:CDS | 40.0% | |
| GTCAAAAGAGTGGTGAGAAA+AGG | - | Chr5:37171896-37171915 | MsG0580026434.01.T01:CDS | 40.0% | |
| TAGGTATGACAGTCTGAGAA+AGG | - | Chr5:37171416-37171435 | MsG0580026434.01.T01:CDS | 40.0% | |
| TGCATCTATTGGTGAAGTGA+TGG | - | Chr5:37172038-37172057 | MsG0580026434.01.T01:CDS | 40.0% | |
| TTTGGGAGAGAAACTGGATT+AGG | - | Chr5:37171462-37171481 | MsG0580026434.01.T01:CDS | 40.0% | |
| ! | CGAGCTTTTCAAAAAAAGGG+TGG | - | Chr5:37171328-37171347 | MsG0580026434.01.T01:CDS | 40.0% |
| ! | GAGAGAAACTGGATTAGGAT+GGG | - | Chr5:37171467-37171486 | MsG0580026434.01.T01:CDS | 40.0% |
| ! | GGAACCCACAACATTTTTCT+TGG | + | Chr5:37171196-37171215 | None:intergenic | 40.0% |
| ! | TCACACAGGTTAGTCATGAA+TGG | + | Chr5:37172225-37172244 | None:intergenic | 40.0% |
| CAGAGGAGAAGAGGTTGAAT+TGG | - | Chr5:37172172-37172191 | MsG0580026434.01.T01:CDS | 45.0% | |
| CGTACCAACACGTTCTCTAT+TGG | + | Chr5:37171310-37171329 | None:intergenic | 45.0% | |
| GATAGTGAGGATGCAAGTGT+TGG | - | Chr5:37171820-37171839 | MsG0580026434.01.T01:intron | 45.0% | |
| GCTGATGTTTAAGCCTGCAA+GGG | - | Chr5:37172125-37172144 | MsG0580026434.01.T01:CDS | 45.0% | |
| GTCTCCAATAGAGAACGTGT+TGG | - | Chr5:37171303-37171322 | MsG0580026434.01.T01:CDS | 45.0% | |
| GTTCCTCCTCACCTAATGAT+GGG | - | Chr5:37171211-37171230 | MsG0580026434.01.T01:CDS | 45.0% | |
| TATTTCCCTACGAGGTTCAG+AGG | - | Chr5:37172155-37172174 | MsG0580026434.01.T01:CDS | 45.0% | |
| TGAGAAAGGATTGGAGAGCA+TGG | - | Chr5:37171430-37171449 | MsG0580026434.01.T01:CDS | 45.0% | |
| TGCTGATGTTTAAGCCTGCA+AGG | - | Chr5:37172124-37172143 | MsG0580026434.01.T01:CDS | 45.0% | |
| TTCCTCCTCACCTAATGATG+GGG | - | Chr5:37171212-37171231 | MsG0580026434.01.T01:CDS | 45.0% | |
| ! | AGGTTAGTCATGAATGGCAG+TGG | + | Chr5:37172219-37172238 | None:intergenic | 45.0% |
| ! | CTCACCACTCTTTTGACCAT+CGG | + | Chr5:37171893-37171912 | None:intergenic | 45.0% |
| ! | GCTACAACAGTGTTGTTCAC+AGG | + | Chr5:37171495-37171514 | None:intergenic | 45.0% |
| ! | GCTGCTGGTAATGCATCTAT+TGG | - | Chr5:37172027-37172046 | MsG0580026434.01.T01:CDS | 45.0% |
| ! | GGAGAGAAACTGGATTAGGA+TGG | - | Chr5:37171466-37171485 | MsG0580026434.01.T01:CDS | 45.0% |
| ! | GGCTAATGGAGAGTACACTT+GGG | - | Chr5:37171711-37171730 | MsG0580026434.01.T01:CDS | 45.0% |
| ! | GGTTAGTCATGAATGGCAGT+GGG | + | Chr5:37172218-37172237 | None:intergenic | 45.0% |
| ! | TGATGATGATGATGAGGCAG+TGG | - | Chr5:37171780-37171799 | MsG0580026434.01.T01:CDS | 45.0% |
| ! | TGGCTAATGGAGAGTACACT+TGG | - | Chr5:37171710-37171729 | MsG0580026434.01.T01:CDS | 45.0% |
| ACACTTGGGCACCATCATGT+GGG | - | Chr5:37171725-37171744 | MsG0580026434.01.T01:CDS | 50.0% | |
| AGAAGGATCTGGCGATAGTG+AGG | - | Chr5:37171807-37171826 | MsG0580026434.01.T01:intron | 50.0% | |
| CATGGCAATACCCCACATGA+TGG | + | Chr5:37171739-37171758 | None:intergenic | 50.0% | |
| CTGTTGTAGCACCTGATGAG+TGG | - | Chr5:37171505-37171524 | MsG0580026434.01.T01:CDS | 50.0% | |
| CTTCTCCTCTGAACCTCGTA+GGG | + | Chr5:37172163-37172182 | None:intergenic | 50.0% | |
| GGTTCCTCCTCACCTAATGA+TGG | - | Chr5:37171210-37171229 | MsG0580026434.01.T01:CDS | 50.0% | |
| GTGGTTAACTCACTGGCGAA+TGG | + | Chr5:37171673-37171692 | None:intergenic | 50.0% | |
| TACACTTGGGCACCATCATG+TGG | - | Chr5:37171724-37171743 | MsG0580026434.01.T01:CDS | 50.0% | |
| TACGAGGTTCAGAGGAGAAG+AGG | - | Chr5:37172163-37172182 | MsG0580026434.01.T01:CDS | 50.0% | |
| TCCTCCTCACCTAATGATGG+GGG | - | Chr5:37171213-37171232 | MsG0580026434.01.T01:CDS | 50.0% | |
| TCTTCTCCTCTGAACCTCGT+AGG | + | Chr5:37172164-37172183 | None:intergenic | 50.0% | |
| TGTAGCACCTGATGAGTGGT+GGG | - | Chr5:37171509-37171528 | MsG0580026434.01.T01:CDS | 50.0% | |
| TTGTAGCACCTGATGAGTGG+TGG | - | Chr5:37171508-37171527 | MsG0580026434.01.T01:CDS | 50.0% | |
| ! | GTTTTCTGCCCCCCATCATT+AGG | + | Chr5:37171225-37171244 | None:intergenic | 50.0% |
| CACTTGGGCACCATCATGTG+GGG | - | Chr5:37171726-37171745 | MsG0580026434.01.T01:CDS | 55.0% | |
| CGTTCTCGCAATGATGCTGC+TGG | - | Chr5:37172012-37172031 | MsG0580026434.01.T01:CDS | 55.0% | |
| GATGAGGCAGTGGCTGTAGA+AGG | - | Chr5:37171790-37171809 | MsG0580026434.01.T01:intron | 55.0% | |
| GATGGCTGAGCTTCACACCA+TGG | - | Chr5:37172056-37172075 | MsG0580026434.01.T01:CDS | 55.0% | |
| GCAGTGGCTGTAGAAGGATC+TGG | - | Chr5:37171796-37171815 | MsG0580026434.01.T01:intron | 55.0% | |
| TTGGTCTCCCACCACTCATC+AGG | + | Chr5:37171519-37171538 | None:intergenic | 55.0% | |
| ! | CCTCCTCACCTAATGATGGG+GGG | - | Chr5:37171214-37171233 | MsG0580026434.01.T01:CDS | 55.0% |
| CCCCCCATCATTAGGTGAGG+AGG | + | Chr5:37171217-37171236 | None:intergenic | 60.0% | |
| CTGCCCCCCATCATTAGGTG+AGG | + | Chr5:37171220-37171239 | None:intergenic | 60.0% | |
| ! | GTGGTGGGAGACCAAACAGC+TGG | - | Chr5:37171524-37171543 | MsG0580026434.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 37171183 | 37172248 | 37171183 | ID=MsG0580026434.01;Name=MsG0580026434.01 |
| Chr5 | mRNA | 37171183 | 37172248 | 37171183 | ID=MsG0580026434.01.T01;Parent=MsG0580026434.01;Name=MsG0580026434.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|326 |
| Chr5 | exon | 37171886 | 37172248 | 37171886 | ID=MsG0580026434.01.T01:exon:15366;Parent=MsG0580026434.01.T01 |
| Chr5 | exon | 37171183 | 37171800 | 37171183 | ID=MsG0580026434.01.T01:exon:15367;Parent=MsG0580026434.01.T01 |
| Chr5 | CDS | 37171886 | 37172248 | 37171886 | ID=MsG0580026434.01.T01:cds;Parent=MsG0580026434.01.T01 |
| Chr5 | CDS | 37171183 | 37171800 | 37171183 | ID=MsG0580026434.01.T01:cds;Parent=MsG0580026434.01.T01 |
| Gene Sequence |
| Protein sequence |
