AlfalfaGEDB | MsG0780036369.01

M. sativa cultivar ZhongmuNo.1 / MsG0780036369.01

KEGG: -
GO: GO:0008168 GO:0046983
PFAM: PF08100

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036369.01.T01	XP_003621331.1	85.315	143	21	0	1	143	1	143	7.63E-84	260

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036369.01.T01	P93324	58.741	143	50	3	8	143	8	148	3.32E-46	156

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036369.01.T01	G7L359	85.315	143	21	0	1	143	1	143	3.64e-84	260

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000399.01	MsG0780036369.01	0.803177	3.948367e-49	1.970893e-46
MsG0680031009.01	MsG0780036369.01	0.802615	5.159154e-49	2.538611e-46
MsG0680033467.01	MsG0780036369.01	0.820432	6.881610e-53	5.444343e-50
MsG0480022833.01	MsG0780036369.01	0.824255	8.912986e-54	7.853057e-51
MsG0580024202.01	MsG0780036369.01	0.836289	1.021011e-56	1.281143e-53
MsG0580024207.01	MsG0780036369.01	0.840130	1.046739e-57	1.476922e-54
MsG0580025023.01	MsG0780036369.01	0.809411	1.917996e-50	1.125347e-47
MsG0580025432.01	MsG0780036369.01	0.801585	8.400462e-49	4.026528e-46
MsG0280008226.01	MsG0780036369.01	0.821717	3.480191e-53	2.853707e-50
MsG0780036367.01	MsG0780036369.01	0.824981	6.012724e-54	5.409954e-51
MsG0780036369.01	MsG0780036370.01	0.821772	3.379670e-53	2.775628e-50
MsG0780036369.01	MsG0780040081.01	0.803333	3.664499e-49	1.836514e-46
MsG0780036369.01	MsG0780040808.01	0.809052	2.289499e-50	1.330431e-47
MsG0780036369.01	MsG0780040991.01	0.801201	1.006932e-48	4.780076e-46
MsG0780036369.01	MsG0880046402.01	0.835737	1.409324e-56	1.738985e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036369.01.T01	MTR_7g011990	85.315	143	21	0	1	143	1	143	9.24e-88	260
MsG0780036369.01.T01	MTR_7g012040	77.931	145	26	2	3	143	2	144	4.81e-76	230
MsG0780036369.01.T01	MTR_7g012020	77.931	145	27	2	3	143	2	145	9.95e-76	226
MsG0780036369.01.T01	MTR_7g012020	77.931	145	27	2	3	143	2	145	5.67e-75	225
MsG0780036369.01.T01	MTR_7g012020	77.931	145	27	2	3	143	2	145	3.53e-74	226
MsG0780036369.01.T01	MTR_7g011900	75.676	148	29	3	1	143	1	146	1.87e-73	224
MsG0780036369.01.T01	MTR_7g012090	65.789	152	42	3	1	143	1	151	6.14e-62	194
MsG0780036369.01.T01	MTR_7g012070	63.158	152	46	3	1	143	1	151	6.76e-62	194
MsG0780036369.01.T01	MTR_2g055940	71.429	126	32	2	21	143	15	139	3.83e-56	179
MsG0780036369.01.T01	MTR_6g060290	59.864	147	40	4	1	143	1	132	3.63e-52	163
MsG0780036369.01.T01	MTR_1g096310	58.042	143	51	3	8	143	8	148	2.39e-47	157
MsG0780036369.01.T01	MTR_1g036460	43.357	143	70	4	8	143	6	144	3.97e-28	106
MsG0780036369.01.T01	MTR_1g036490	41.096	146	75	4	5	143	3	144	1.26e-26	102
MsG0780036369.01.T01	MTR_1g050220	46.552	116	53	2	26	134	6	119	2.94e-24	95.1
MsG0780036369.01.T01	MTR_1g036510	43.939	132	63	4	19	143	31	158	4.95e-24	94.0
MsG0780036369.01.T01	MTR_1g036510	43.939	132	63	4	19	143	31	158	8.93e-24	94.0
MsG0780036369.01.T01	MTR_3g021440	46.825	126	58	5	21	140	22	144	1.37e-23	94.4
MsG0780036369.01.T01	MTR_1g036510	43.939	132	63	4	19	143	31	158	1.95e-23	94.4
MsG0780036369.01.T01	MTR_3g021430	45.045	111	53	4	35	140	24	131	2.98e-18	79.7
MsG0780036369.01.T01	MTR_3g092900	38.843	121	68	3	5	121	2	120	3.10e-18	79.7
MsG0780036369.01.T01	MTR_5g098170	37.615	109	62	3	19	121	12	120	9.93e-14	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036369.01.T01	AT5G54160	42.718	103	57	1	21	121	16	118	3.34e-18	79.7
MsG0780036369.01.T01	AT1G21100	37.600	125	65	4	16	129	14	136	2.49e-14	68.9
MsG0780036369.01.T01	AT1G21130	38.095	126	65	4	15	129	13	136	4.03e-14	68.6
MsG0780036369.01.T01	AT1G21130	38.095	126	65	4	15	129	13	136	4.04e-14	67.8

Find 28 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAACTTGTTTGAAATCAT+TGG	0.292345	7:-7168483	MsG0780036369.01.T01:CDS
GGTAGCATAGTGAGTGTTTA+TGG	0.300569	7:-7168321	MsG0780036369.01.T01:CDS
ATGTGTCATCCTGCTTTGAT+AGG	0.301521	7:-7168222	MsG0780036369.01.T01:CDS
AACTGCTGGAAACACTAAAT+TGG	0.308710	7:+7168524	None:intergenic
AATAGTGACCATGGTTATCT+TGG	0.387856	7:-7168261	MsG0780036369.01.T01:CDS
AACACTCTGATTTGCCGAAT+CGG	0.420138	7:-7168413	MsG0780036369.01.T01:CDS
GTTTATGGGATCACACCTTC+TGG	0.427376	7:-7168306	MsG0780036369.01.T01:CDS
AACATACGCTCGAGCCGATT+CGG	0.429975	7:+7168399	None:intergenic
CACATGGCTTTCTTTCTCAC+TGG	0.430420	7:+7168608	None:intergenic
GTATCACTGTCATCTATTTG+TGG	0.439622	7:+7168570	None:intergenic
CACTATGCTACCATCATCAT+TGG	0.442096	7:+7168332	None:intergenic
GTAGCATAGTGAGTGTTTAT+GGG	0.464079	7:-7168320	MsG0780036369.01.T01:CDS
ATTGCAGCATTAAGAACTGC+TGG	0.480012	7:+7168510	None:intergenic
TATCACTGTCATCTATTTGT+GGG	0.481354	7:+7168571	None:intergenic
GCTTTCTTTCTCACTGGCAT+AGG	0.498989	7:+7168614	None:intergenic
TGTGTCATCCTGCTTTGATA+GGG	0.514622	7:-7168221	MsG0780036369.01.T01:CDS
ACACTCTCAGCTATGGTACT+TGG	0.515175	7:-7168549	MsG0780036369.01.T01:CDS
TTGTTTGAAATCATTGGTGA+TGG	0.517050	7:-7168477	MsG0780036369.01.T01:CDS
ATCCTGCTTTGATAGGGTTG+TGG	0.524365	7:-7168215	MsG0780036369.01.T01:CDS
GGCAAATCAGAGTGTTGAGT+TGG	0.551660	7:+7168420	None:intergenic
TCTAATCGGACCAATGATGA+TGG	0.567171	7:-7168342	MsG0780036369.01.T01:CDS
CAGTGATACACTCTCAGCTA+TGG	0.574081	7:-7168556	MsG0780036369.01.T01:CDS
TACCACAACCCTATCAAAGC+AGG	0.575416	7:+7168213	None:intergenic
TAACAAAAGTATTTACCAGA+AGG	0.598640	7:+7168291	None:intergenic
ATCACTGTCATCTATTTGTG+GGG	0.627306	7:+7168572	None:intergenic
TATGATGAAAATAGTGACCA+TGG	0.659727	7:-7168270	MsG0780036369.01.T01:CDS
GGGTGTGAGATTCAATCACA+TGG	0.675697	7:+7168592	None:intergenic
TGAATGAGCCAAGATAACCA+TGG	0.765614	7:+7168253	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTCTTTTGTCTATTTCTAAT+CGG	-	Chr7:7168477-7168496	MsG0780036369.01.T01:CDS	25.0%
!!!	TAACAAAAGTATTTACCAGA+AGG	+	Chr7:7168545-7168564	None:intergenic	25.0%
	TATCACTGTCATCTATTTGT+GGG	+	Chr7:7168265-7168284	None:intergenic	30.0%
	TATGATGAAAATAGTGACCA+TGG	-	Chr7:7168563-7168582	MsG0780036369.01.T01:CDS	30.0%
!	CTCAACTTGTTTGAAATCAT+TGG	-	Chr7:7168350-7168369	MsG0780036369.01.T01:CDS	30.0%
!	TTGTTTGAAATCATTGGTGA+TGG	-	Chr7:7168356-7168375	MsG0780036369.01.T01:CDS	30.0%
	AACTGCTGGAAACACTAAAT+TGG	+	Chr7:7168312-7168331	None:intergenic	35.0%
	AATAGTGACCATGGTTATCT+TGG	-	Chr7:7168572-7168591	MsG0780036369.01.T01:CDS	35.0%
	ATCACTGTCATCTATTTGTG+GGG	+	Chr7:7168264-7168283	None:intergenic	35.0%
	GTAGCATAGTGAGTGTTTAT+GGG	-	Chr7:7168513-7168532	MsG0780036369.01.T01:CDS	35.0%
	GTATCACTGTCATCTATTTG+TGG	+	Chr7:7168266-7168285	None:intergenic	35.0%
	ATGTGTCATCCTGCTTTGAT+AGG	-	Chr7:7168611-7168630	MsG0780036369.01.T01:CDS	40.0%
	ATTGCAGCATTAAGAACTGC+TGG	+	Chr7:7168326-7168345	None:intergenic	40.0%
	CACTATGCTACCATCATCAT+TGG	+	Chr7:7168504-7168523	None:intergenic	40.0%
	GGTAGCATAGTGAGTGTTTA+TGG	-	Chr7:7168512-7168531	MsG0780036369.01.T01:CDS	40.0%
	TCTAATCGGACCAATGATGA+TGG	-	Chr7:7168491-7168510	MsG0780036369.01.T01:CDS	40.0%
	TGAATGAGCCAAGATAACCA+TGG	+	Chr7:7168583-7168602	None:intergenic	40.0%
	TGTGTCATCCTGCTTTGATA+GGG	-	Chr7:7168612-7168631	MsG0780036369.01.T01:CDS	40.0%
!	AACACTCTGATTTGCCGAAT+CGG	-	Chr7:7168420-7168439	MsG0780036369.01.T01:CDS	40.0%
	ACACTCTCAGCTATGGTACT+TGG	-	Chr7:7168284-7168303	MsG0780036369.01.T01:CDS	45.0%
	ATCCTGCTTTGATAGGGTTG+TGG	-	Chr7:7168618-7168637	MsG0780036369.01.T01:CDS	45.0%
	CACATGGCTTTCTTTCTCAC+TGG	+	Chr7:7168228-7168247	None:intergenic	45.0%
	CAGTGATACACTCTCAGCTA+TGG	-	Chr7:7168277-7168296	MsG0780036369.01.T01:CDS	45.0%
	GCTTTCTTTCTCACTGGCAT+AGG	+	Chr7:7168222-7168241	None:intergenic	45.0%
	GGGTGTGAGATTCAATCACA+TGG	+	Chr7:7168244-7168263	None:intergenic	45.0%
	GTTTATGGGATCACACCTTC+TGG	-	Chr7:7168527-7168546	MsG0780036369.01.T01:CDS	45.0%
	TACCACAACCCTATCAAAGC+AGG	+	Chr7:7168623-7168642	None:intergenic	45.0%
!!	GGCAAATCAGAGTGTTGAGT+TGG	+	Chr7:7168416-7168435	None:intergenic	45.0%
	AACATACGCTCGAGCCGATT+CGG	+	Chr7:7168437-7168456	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	7168212	7168643	7168212	ID=MsG0780036369.01;Name=MsG0780036369.01
Chr7	mRNA	7168212	7168643	7168212	ID=MsG0780036369.01.T01;Parent=MsG0780036369.01;Name=MsG0780036369.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|143
Chr7	exon	7168212	7168643	7168212	ID=MsG0780036369.01.T01:exon:3846;Parent=MsG0780036369.01.T01
Chr7	CDS	7168212	7168643	7168212	ID=MsG0780036369.01.T01:cds;Parent=MsG0780036369.01.T01

Gene Sequence

>MsG0780036369.01.T01

ATGAGTTCCTATGCCAGTGAGAAAGAAAGCCATGTGATTGAATCTCACACCCCACAAATAGATGACAGTGATACACTCTCAGCTATGGTACTTGGTGCCAATTTAGTGTTTCCAGCAGTTCTTAATGCTGCAATTCAACTCAACTTGTTTGAAATCATTGGTGATGGATTCATGTCATCTTTTGAAATTGCTTCAAAGTTACCAACTCAACACTCTGATTTGCCGAATCGGCTCGAGCGTATGTTGCGTTTGCTTGAAAGCTACTCTCTTTTGTCTATTTCTAATCGGACCAATGATGATGGTAGCATAGTGAGTGTTTATGGGATCACACCTTCTGGTAAATACTTTTGTTATGATGAAAATAGTGACCATGGTTATCTTGGCTCATTCACATCATTTATGTGTCATCCTGCTTTGATAGGGTTGTGGTAA

Protein sequence

>MsG0780036369.01.T01

MSSYASEKESHVIESHTPQIDDSDTLSAMVLGANLVFPAVLNAAIQLNLFEIIGDGFMSSFEIASKLPTQHSDLPNRLERMLRLLESYSLLSISNRTNDDGSIVSVYGITPSGKYFCYDENSDHGYLGSFTSFMCHPALIGLW*