AlfalfaGEDB | MsG0780039620.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039620.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039620.01.T01	MCH97906.1	88.148	135	16	0	7	141	1	135	1.10E-82	250

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039620.01.T01	Q8LG03	46.528	144	77	0	6	149	39	182	1.57E-33	124

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039620.01.T01	A0A392NDF3	88.148	135	16	0	7	141	1	135	5.25e-83	250

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005559.01	MsG0780039620.01	0.806224	9.127152e-50	4.927182e-47
MsG0180006023.01	MsG0780039620.01	0.809418	1.911228e-50	1.121575e-47
MsG0280010614.01	MsG0780039620.01	0.822181	2.717525e-53	2.257807e-50
MsG0280010735.01	MsG0780039620.01	0.802947	4.405685e-49	2.186185e-46
MsG0280011085.01	MsG0780039620.01	0.836292	1.019295e-56	1.279093e-53
MsG0480020445.01	MsG0780039620.01	0.825428	4.712345e-54	4.293810e-51
MsG0480021134.01	MsG0780039620.01	0.818472	1.925716e-52	1.442901e-49
MsG0480022479.01	MsG0780039620.01	0.838933	2.141979e-57	2.912574e-54
MsG0580024682.01	MsG0780039620.01	0.806437	8.231501e-50	4.467636e-47
MsG0580024770.01	MsG0780039620.01	0.815581	8.589066e-52	5.945012e-49
MsG0580025907.01	MsG0780039620.01	0.804081	2.562950e-49	1.309433e-46
MsG0580027129.01	MsG0780039620.01	0.800530	1.380428e-48	6.442578e-46
MsG0680030436.01	MsG0780039620.01	0.813981	1.943910e-51	1.288211e-48
MsG0280010459.01	MsG0780039620.01	0.806125	9.572369e-50	5.154437e-47
MsG0680035681.01	MsG0780039620.01	0.807556	4.770881e-50	2.665398e-47
MsG0680035737.01	MsG0780039620.01	0.817205	3.720900e-52	2.692439e-49
MsG0780036906.01	MsG0780039620.01	0.812100	5.023577e-51	3.165028e-48
MsG0780039620.01	MsG0780041310.01	0.807139	5.847595e-50	3.231710e-47
MsG0780039620.01	MsG0880043921.01	0.831898	1.285828e-55	1.414667e-52
MsG0780039620.01	MsG0880044546.01	0.802098	6.591856e-49	3.201127e-46
MsG0780039620.01	MsG0880046615.01	0.813385	2.629143e-51	1.714514e-48
MsG0780039620.01	MsG0880046669.01	0.813246	2.819967e-51	1.831853e-48
MsG0780039620.01	MsG0880047497.01	0.804551	2.045672e-49	1.057825e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039620.01.T01	MTR_7g076190	85.517	145	20	1	6	150	46	189	5.77e-85	254

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039620.01.T01	AT4G00755	46.528	144	77	0	6	149	39	182	1.60e-34	124
MsG0780039620.01.T01	AT4G00755	46.528	144	77	0	6	149	39	182	1.60e-34	124

Find 35 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGTCCATTTACTCCATTC+TGG	0.220340	7:-66786398	None:intergenic
CTGAACCAGAATGGAGTAAA+TGG	0.275177	7:+66786393	MsG0780039620.01.T01:CDS
TTGTCCACTACTTGACCAAT+AGG	0.353318	7:-66786625	None:intergenic
GAGCTGGACTTTCCATAGAT+TGG	0.386248	7:-66786437	None:intergenic
GCAATGATTAACTGATGTTA+TGG	0.396359	7:+66786286	None:intergenic
GGTTGGATCTAGCCAATCTA+TGG	0.397982	7:+66786425	MsG0780039620.01.T01:CDS
AACAGGAGATAAGCAGGATT+TGG	0.417619	7:-66786493	None:intergenic
GTTAATGTCTCGGGTACATT+GGG	0.429167	7:-66786654	None:intergenic
CTGCTTATCTCCTGTTGTTG+AGG	0.449921	7:+66786500	MsG0780039620.01.T01:CDS
TGGTTTGCAGTGGTTACAAA+TGG	0.465133	7:+66786306	MsG0780039620.01.T01:CDS
CTATGATCTTTCTGAAGAGC+TGG	0.481335	7:-66786453	None:intergenic
GCTATGCAATCCTCAACAAC+AGG	0.484027	7:-66786510	None:intergenic
CTCAACAACAGGAGATAAGC+AGG	0.486568	7:-66786499	None:intergenic
GATTGCATAGCTAAGGCAAT+TGG	0.489466	7:+66786522	MsG0780039620.01.T01:CDS
AAGTGGTATGTTAATGTCTC+GGG	0.494932	7:-66786663	None:intergenic
TGTTAATGTCTCGGGTACAT+TGG	0.507386	7:-66786655	None:intergenic
CAAGTGGTATGTTAATGTCT+CGG	0.516076	7:-66786664	None:intergenic
AATCAAATTCAGCCTTTCCA+AGG	0.525422	7:+66786713	MsG0780039620.01.T01:CDS
GAAGATTTCAAATATCACCT+TGG	0.529539	7:-66786730	None:intergenic
TAGCACTGATAATTTCCCTC+AGG	0.534992	7:+66786551	MsG0780039620.01.T01:CDS
TCAAGCACAACTGCTTAGAA+AGG	0.538427	7:-66786329	None:intergenic
TGTTGAGGATTGCATAGCTA+AGG	0.558055	7:+66786515	MsG0780039620.01.T01:CDS
GTAAATGGACACACAGAGGT+TGG	0.559495	7:+66786408	MsG0780039620.01.T01:CDS
CAAGCAACTCTAGATAATTG+AGG	0.563729	7:-66786360	None:intergenic
TGTAATAACACATATTTGAG+AGG	0.564766	7:-66786688	None:intergenic
GTTGTTGAACTGAACCAGAA+TGG	0.565614	7:+66786384	MsG0780039620.01.T01:CDS
CACATATTTGAGAGGTCAAG+TGG	0.574105	7:-66786680	None:intergenic
TTTCAAATATCACCTTGGAA+AGG	0.578942	7:-66786725	None:intergenic
GAGGAAAGAGACAACATTGG+TGG	0.593992	7:+66786594	MsG0780039620.01.T01:CDS
GAGCATTGATCACACTCTTG+AGG	0.611911	7:+66786575	MsG0780039620.01.T01:CDS
GTGTGATCAATGCTCTCCTG+AGG	0.612736	7:-66786567	None:intergenic
TGTGATCAATGCTCTCCTGA+GGG	0.617504	7:-66786566	None:intergenic
ACTGATGTTATGGTTTGCAG+TGG	0.653450	7:+66786296	None:intergenic
CTTGAGGAAAGAGACAACAT+TGG	0.703612	7:+66786591	MsG0780039620.01.T01:CDS
TGGAGTAAATGGACACACAG+AGG	0.727277	7:+66786404	MsG0780039620.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGTAATAACACATATTTGAG+AGG	-	Chr7:66786691-66786710	None:intergenic	25.0%
	GAAGATTTCAAATATCACCT+TGG	-	Chr7:66786733-66786752	None:intergenic	30.0%
	TTTCAAATATCACCTTGGAA+AGG	-	Chr7:66786728-66786747	None:intergenic	30.0%
	AAGTGGTATGTTAATGTCTC+GGG	-	Chr7:66786666-66786685	None:intergenic	35.0%
	AATCAAATTCAGCCTTTCCA+AGG	+	Chr7:66786713-66786732	MsG0780039620.01.T01:CDS	35.0%
	CAAGCAACTCTAGATAATTG+AGG	-	Chr7:66786363-66786382	None:intergenic	35.0%
	CAAGTGGTATGTTAATGTCT+CGG	-	Chr7:66786667-66786686	None:intergenic	35.0%
!	TTTTCCTATTGGTCAAGTAG+TGG	+	Chr7:66786621-66786640	MsG0780039620.01.T01:CDS	35.0%
!!	TTGGTGGTAGATTTTCCTAT+TGG	+	Chr7:66786610-66786629	MsG0780039620.01.T01:CDS	35.0%
	AACAGGAGATAAGCAGGATT+TGG	-	Chr7:66786496-66786515	None:intergenic	40.0%
	CACATATTTGAGAGGTCAAG+TGG	-	Chr7:66786683-66786702	None:intergenic	40.0%
	CTGAACCAGAATGGAGTAAA+TGG	+	Chr7:66786393-66786412	MsG0780039620.01.T01:CDS	40.0%
	CTTGAGGAAAGAGACAACAT+TGG	+	Chr7:66786591-66786610	MsG0780039620.01.T01:CDS	40.0%
	GATTGCATAGCTAAGGCAAT+TGG	+	Chr7:66786522-66786541	MsG0780039620.01.T01:CDS	40.0%
	GTTAATGTCTCGGGTACATT+GGG	-	Chr7:66786657-66786676	None:intergenic	40.0%
	GTTGTTGAACTGAACCAGAA+TGG	+	Chr7:66786384-66786403	MsG0780039620.01.T01:CDS	40.0%
	TCAAGCACAACTGCTTAGAA+AGG	-	Chr7:66786332-66786351	None:intergenic	40.0%
	TGTGTCCATTTACTCCATTC+TGG	-	Chr7:66786401-66786420	None:intergenic	40.0%
	TGTTAATGTCTCGGGTACAT+TGG	-	Chr7:66786658-66786677	None:intergenic	40.0%
	TGTTGAGGATTGCATAGCTA+AGG	+	Chr7:66786515-66786534	MsG0780039620.01.T01:CDS	40.0%
	TTGTCCACTACTTGACCAAT+AGG	-	Chr7:66786628-66786647	None:intergenic	40.0%
!	CTATGATCTTTCTGAAGAGC+TGG	-	Chr7:66786456-66786475	None:intergenic	40.0%
!	TGGTTTGCAGTGGTTACAAA+TGG	+	Chr7:66786306-66786325	MsG0780039620.01.T01:CDS	40.0%
!!	TAGCACTGATAATTTCCCTC+AGG	+	Chr7:66786551-66786570	MsG0780039620.01.T01:CDS	40.0%
	CTCAACAACAGGAGATAAGC+AGG	-	Chr7:66786502-66786521	None:intergenic	45.0%
	CTGCTTATCTCCTGTTGTTG+AGG	+	Chr7:66786500-66786519	MsG0780039620.01.T01:CDS	45.0%
	GAGCTGGACTTTCCATAGAT+TGG	-	Chr7:66786440-66786459	None:intergenic	45.0%
	GAGGAAAGAGACAACATTGG+TGG	+	Chr7:66786594-66786613	MsG0780039620.01.T01:CDS	45.0%
	GCTATGCAATCCTCAACAAC+AGG	-	Chr7:66786513-66786532	None:intergenic	45.0%
	GGTTGGATCTAGCCAATCTA+TGG	+	Chr7:66786425-66786444	MsG0780039620.01.T01:CDS	45.0%
	GTAAATGGACACACAGAGGT+TGG	+	Chr7:66786408-66786427	MsG0780039620.01.T01:CDS	45.0%
	TGGAGTAAATGGACACACAG+AGG	+	Chr7:66786404-66786423	MsG0780039620.01.T01:CDS	45.0%
	TGTGATCAATGCTCTCCTGA+GGG	-	Chr7:66786569-66786588	None:intergenic	45.0%
!!	GAGCATTGATCACACTCTTG+AGG	+	Chr7:66786575-66786594	MsG0780039620.01.T01:CDS	45.0%
	GTGTGATCAATGCTCTCCTG+AGG	-	Chr7:66786570-66786589	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	66786300	66786782	66786300	ID=MsG0780039620.01;Name=MsG0780039620.01
Chr7	mRNA	66786300	66786782	66786300	ID=MsG0780039620.01.T01;Parent=MsG0780039620.01;Name=MsG0780039620.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|160
Chr7	exon	66786300	66786782	66786300	ID=MsG0780039620.01.T01:exon:13183;Parent=MsG0780039620.01.T01
Chr7	CDS	66786300	66786782	66786300	ID=MsG0780039620.01.T01:cds;Parent=MsG0780039620.01.T01

Gene Sequence

>MsG0780039620.01.T01

ATGTTATGGTTTGCAGTGGTTACAAATGGCCTTTCTAAGCAGTTGTGCTTGAGAATGTTTCCTCAATTATCTAGAGTTGCTTGTGTTGTTGAACTGAACCAGAATGGAGTAAATGGACACACAGAGGTTGGATCTAGCCAATCTATGGAAAGTCCAGCTCTTCAGAAAGATCATAGAGTCTATAGTTATTTTGCCAAATCCTGCTTATCTCCTGTTGTTGAGGATTGCATAGCTAAGGCAATTGGTGCTTCTAGCACTGATAATTTCCCTCAGGAGAGCATTGATCACACTCTTGAGGAAAGAGACAACATTGGTGGTAGATTTTCCTATTGGTCAAGTAGTGGACAAAGTAATCCCAATGTACCCGAGACATTAACATACCACTTGACCTCTCAAATATGTGTTATTACAGAAATCAAATTCAGCCTTTCCAAGGTGATATTTGAAATCTTCAGAAACTATGTGCTCGACTCATTTATTTGA

Protein sequence

>MsG0780039620.01.T01

MLWFAVVTNGLSKQLCLRMFPQLSRVACVVELNQNGVNGHTEVGSSQSMESPALQKDHRVYSYFAKSCLSPVVEDCIAKAIGASSTDNFPQESIDHTLEERDNIGGRFSYWSSSGQSNPNVPETLTYHLTSQICVITEIKFSLSKVIFEIFRNYVLDSFI*