AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044546.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044546.01.T01 MTR_8g464390 83.838 99 12 1 7 105 37 131 2.30e-56 181
MsG0880044546.01.T01 MTR_2g080950 91.358 81 7 0 7 87 37 117 3.11e-52 170
MsG0880044546.01.T01 MTR_8g464390 82.143 84 11 1 22 105 1 80 7.49e-44 147
MsG0880044546.01.T01 MTR_6g080710 69.474 95 23 2 7 99 37 127 6.59e-43 141
MsG0880044546.01.T01 MTR_2g080950 92.424 66 5 0 22 87 1 66 1.29e-40 139
MsG0880044546.01.T01 MTR_2g030230 48.936 94 45 2 9 99 41 134 1.52e-23 92.4
MsG0880044546.01.T01 MTR_8g088320 44.681 94 49 2 9 99 32 125 3.63e-22 88.6
MsG0880044546.01.T01 MTR_8g038400 45.098 102 53 2 9 107 40 141 1.57e-21 87.4
MsG0880044546.01.T01 MTR_7g020860 40.157 127 40 4 9 104 525 646 3.91e-21 87.0
MsG0880044546.01.T01 MTR_7g020860 57.576 66 26 1 9 72 525 590 4.15e-20 84.3
MsG0880044546.01.T01 MTR_4g074170 45.098 102 38 2 9 92 502 603 1.07e-19 83.2
MsG0880044546.01.T01 MTR_8g067090 46.988 83 38 1 7 89 31 107 1.34e-19 82.4
MsG0880044546.01.T01 MTR_8g471120 46.988 83 38 1 7 89 31 107 1.34e-19 82.4
MsG0880044546.01.T01 MTR_8g067080 55.000 60 27 0 7 66 31 90 3.17e-19 81.3
MsG0880044546.01.T01 MTR_8g067080 55.000 60 27 0 7 66 31 90 3.19e-19 81.3
MsG0880044546.01.T01 MTR_8g471110 55.000 60 27 0 7 66 31 90 3.19e-19 81.3
MsG0880044546.01.T01 MTR_8g100100 60.656 61 22 1 9 67 523 583 1.14e-18 80.1
MsG0880044546.01.T01 MTR_8g100100 60.656 61 22 1 9 67 517 577 1.19e-18 80.1
MsG0880044546.01.T01 MTR_3g017500 46.465 99 44 4 9 98 44 142 5.05e-18 77.8
MsG0880044546.01.T01 MTR_3g017500 46.465 99 44 4 9 98 74 172 6.87e-18 77.8
MsG0880044546.01.T01 MTR_3g098680 57.377 61 24 1 9 67 520 580 6.48e-17 75.1
MsG0880044546.01.T01 MTR_1g069000 50.847 59 29 0 7 65 25 83 1.30e-16 74.3
MsG0880044546.01.T01 MTR_7g099830 49.153 59 30 0 7 65 25 83 2.10e-16 73.6
MsG0880044546.01.T01 MTR_2g080950 87.097 31 4 0 57 87 1 31 4.22e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044546.01.T01 AT5G54310 93.827 81 5 0 7 87 37 117 1.12e-53 174
MsG0880044546.01.T01 AT3G17660 71.605 81 23 0 7 87 37 117 1.06e-41 135
MsG0880044546.01.T01 AT3G17660 71.605 81 23 0 7 87 37 117 4.28e-41 135
MsG0880044546.01.T01 AT3G17660 71.605 81 23 0 7 87 37 117 7.51e-41 135
MsG0880044546.01.T01 AT3G17660 68.182 66 21 0 22 87 1 66 1.16e-29 105
MsG0880044546.01.T01 AT3G17660 53.086 81 28 1 7 87 37 107 3.11e-25 94.7
MsG0880044546.01.T01 AT4G05330 49.474 95 39 3 9 94 38 132 2.11e-23 92.0
MsG0880044546.01.T01 AT4G05330 49.474 95 39 3 9 94 38 132 2.11e-23 92.0
MsG0880044546.01.T01 AT4G21160 43.119 109 53 3 9 108 38 146 6.32e-22 88.2
MsG0880044546.01.T01 AT4G21160 43.119 109 53 3 9 108 38 146 6.32e-22 88.2
MsG0880044546.01.T01 AT4G21160 43.119 109 53 3 9 108 38 146 6.32e-22 88.2
MsG0880044546.01.T01 AT4G21160 43.119 109 53 3 9 108 38 146 6.32e-22 88.2
MsG0880044546.01.T01 AT5G13300 57.377 61 24 1 9 67 524 584 2.38e-19 82.0
MsG0880044546.01.T01 AT5G61980 62.295 61 21 1 9 67 521 581 2.83e-19 82.0
MsG0880044546.01.T01 AT4G05330 46.875 96 42 3 8 94 2 97 2.87e-19 80.5
MsG0880044546.01.T01 AT5G46750 54.098 61 28 0 7 67 31 91 2.29e-18 79.0
MsG0880044546.01.T01 AT3G53710 53.333 60 28 0 7 66 25 84 2.74e-18 79.0
MsG0880044546.01.T01 AT3G53710 53.333 60 28 0 7 66 25 84 2.74e-18 79.0
MsG0880044546.01.T01 AT3G53710 53.333 60 28 0 7 66 25 84 2.74e-18 79.0
MsG0880044546.01.T01 AT1G60860 44.330 97 52 1 9 103 319 415 3.91e-18 78.6
MsG0880044546.01.T01 AT4G17890 45.783 83 39 1 7 89 34 110 4.41e-18 78.2
MsG0880044546.01.T01 AT1G60860 44.330 97 52 1 9 103 379 475 4.48e-18 78.6
MsG0880044546.01.T01 AT4G17890 45.783 83 39 1 7 89 34 110 4.89e-18 78.2
MsG0880044546.01.T01 AT1G60860 44.330 97 52 1 9 103 490 586 5.10e-18 78.2
MsG0880044546.01.T01 AT2G35210 52.459 61 29 0 7 67 31 91 1.19e-17 77.0
MsG0880044546.01.T01 AT3G07940 43.243 111 43 6 9 108 70 171 1.25e-17 77.0
MsG0880044546.01.T01 AT2G35210 52.459 61 29 0 7 67 31 91 1.31e-17 77.0
MsG0880044546.01.T01 AT3G07940 43.243 111 43 6 9 108 70 171 1.55e-17 75.5
MsG0880044546.01.T01 AT2G37550 52.542 59 28 0 7 65 25 83 1.72e-17 76.6
MsG0880044546.01.T01 AT2G37550 52.542 59 28 0 7 65 25 83 1.72e-17 76.6
MsG0880044546.01.T01 AT1G10870 50.725 69 32 1 9 75 490 558 1.79e-17 76.6
MsG0880044546.01.T01 AT1G10870 50.725 69 32 1 9 75 490 558 1.82e-17 76.6

Find 21 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATAAATGCAACCTGCTC+TGG 0.291922 8:-44189648 None:intergenic
TTTATTTGCATGAGTTGTTC+AGG 0.310667 8:+44188826 MsG0880044546.01.T01:CDS
ATGAGAGAGCAAATAGTTAC+TGG 0.354535 8:+44189777 MsG0880044546.01.T01:CDS
GTAGGTTCGTTCTGCTACTT+TGG 0.370771 8:+44189614 MsG0880044546.01.T01:intron
CAGGAATACATCGAAGTCTT+GGG 0.391033 8:+44188845 MsG0880044546.01.T01:CDS
GGACACCTGGCTCCCAGAGC+AGG 0.422168 8:+44189635 MsG0880044546.01.T01:CDS
TGCAACCTGCTCTGGGAGCC+AGG 0.424491 8:-44189640 None:intergenic
TCAGGAATACATCGAAGTCT+TGG 0.467211 8:+44188844 MsG0880044546.01.T01:CDS
CCAACTCTGTCATAATTTGT+CGG 0.480013 8:-44189812 None:intergenic
GTTCTGCTACTTTGGACACC+TGG 0.506568 8:+44189622 MsG0880044546.01.T01:CDS
AGATGGGCTAGTGTCAATCT+TGG 0.512988 8:+44188799 MsG0880044546.01.T01:CDS
GAATAAATGCAACCTGCTCT+GGG 0.526047 8:-44189647 None:intergenic
TGATTTGCAGAGGACCAAGA+TGG 0.533820 8:+44188782 MsG0880044546.01.T01:CDS
AGATTGACACTAGCCCATCT+TGG 0.538185 8:-44188796 None:intergenic
TCTTGGGGTACATATTTCAA+AGG 0.571580 8:+44188861 MsG0880044546.01.T01:CDS
CCGACAAATTATGACAGAGT+TGG 0.575509 8:+44189812 MsG0880044546.01.T01:CDS
GATTTGCAGAGGACCAAGAT+GGG 0.581489 8:+44188783 MsG0880044546.01.T01:CDS
ATAAACTAACATACTTTGCA+CGG 0.585290 8:-44189850 None:intergenic
TGAGAGAGCAAATAGTTACT+GGG 0.605138 8:+44189778 MsG0880044546.01.T01:CDS
GTTTATACCTTGAACCAGAG+TGG 0.636982 8:+44189867 MsG0880044546.01.T01:CDS
AGGAATACATCGAAGTCTTG+GGG 0.657722 8:+44188846 MsG0880044546.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACAGAAATCCAATAAAAA+AGG - Chr8:44189460-44189479 None:intergenic 20.0%
!! TCTTTAAATCCACATTAAAA+AGG - Chr8:44189314-44189333 None:intergenic 20.0%
!! TTTCAATAATGTTGCAAATT+TGG - Chr8:44189215-44189234 None:intergenic 20.0%
!!! ATTTTTTTTAAAAACCACTC+TGG - Chr8:44189884-44189903 None:intergenic 20.0%
! ATAAACTAACATACTTTGCA+CGG - Chr8:44189853-44189872 None:intergenic 25.0%
! ATTATTACATGCTTACTTCT+TGG + Chr8:44189584-44189603 MsG0880044546.01.T01:intron 25.0%
! ATTGAAATGCATTCTTGAAT+TGG + Chr8:44189228-44189247 MsG0880044546.01.T01:intron 25.0%
! TACAAAATTCAATAACCACA+AGG - Chr8:44189700-44189719 None:intergenic 25.0%
! TATGATCTTGATGCTTAAAA+AGG + Chr8:44188939-44188958 MsG0880044546.01.T01:intron 25.0%
! TCAATGTAAGTGAATCTAAA+TGG + Chr8:44189668-44189687 MsG0880044546.01.T01:intron 25.0%
!!! TCATTGTTTTAAACAGCTAT+GGG + Chr8:44189752-44189771 MsG0880044546.01.T01:intron 25.0%
!!! TTCATTGTTTTAAACAGCTA+TGG + Chr8:44189751-44189770 MsG0880044546.01.T01:intron 25.0%
AATCCAATAAAAAAGGACCT+AGG - Chr8:44189453-44189472 None:intergenic 30.0%
AATGTGGATTTAAAGATTGC+TGG + Chr8:44189318-44189337 MsG0880044546.01.T01:intron 30.0%
ATGTGGATTTAAAGATTGCT+GGG + Chr8:44189319-44189338 MsG0880044546.01.T01:intron 30.0%
CAATGTTATGTTCCTTTGTA+TGG + Chr8:44188887-44188906 MsG0880044546.01.T01:intron 30.0%
TCAACAATTCTACCATACAA+AGG - Chr8:44188902-44188921 None:intergenic 30.0%
TCTAACCGTATATTGACAAA+CGG - Chr8:44189285-44189304 None:intergenic 30.0%
TTGTATGGTAGAATTGTTGA+GGG + Chr8:44188902-44188921 MsG0880044546.01.T01:intron 30.0%
TTTATTTGCATGAGTTGTTC+AGG + Chr8:44188826-44188845 MsG0880044546.01.T01:CDS 30.0%
TTTGTATGGTAGAATTGTTG+AGG + Chr8:44188901-44188920 MsG0880044546.01.T01:intron 30.0%
! AGACTGTTACCTTTTTAATG+TGG + Chr8:44189302-44189321 MsG0880044546.01.T01:intron 30.0%
! TGACTTGAACTTTTGTTGAT+AGG + Chr8:44189256-44189275 MsG0880044546.01.T01:intron 30.0%
! TGAGTTTCTTGTTTGCATTA+TGG + Chr8:44189067-44189086 MsG0880044546.01.T01:intron 30.0%
!! TTACTTCTTGGTTTTTCTGT+AGG + Chr8:44189596-44189615 MsG0880044546.01.T01:intron 30.0%
!!! ATGGAAATTTTGATTTGCAG+AGG + Chr8:44188772-44188791 MsG0880044546.01.T01:CDS 30.0%
!!! TGTTTTGAGATGCTATTGTT+CGG + Chr8:44189148-44189167 MsG0880044546.01.T01:intron 30.0%
ATGAGAGAGCAAATAGTTAC+TGG + Chr8:44189777-44189796 MsG0880044546.01.T01:CDS 35.0%
ATGTTATTCCAGTTGTGTTG+TGG + Chr8:44189092-44189111 MsG0880044546.01.T01:intron 35.0%
GGAATCCGTTTGTCAATATA+CGG + Chr8:44189277-44189296 MsG0880044546.01.T01:intron 35.0%
TCTTGGGGTACATATTTCAA+AGG + Chr8:44188861-44188880 MsG0880044546.01.T01:CDS 35.0%
TGAGAGAGCAAATAGTTACT+GGG + Chr8:44189778-44189797 MsG0880044546.01.T01:CDS 35.0%
TGTTATTCCAGTTGTGTTGT+GGG + Chr8:44189093-44189112 MsG0880044546.01.T01:intron 35.0%
TTAATATCCCACAACACAAC+TGG - Chr8:44189103-44189122 None:intergenic 35.0%
! CCAACTCTGTCATAATTTGT+CGG - Chr8:44189815-44189834 None:intergenic 35.0%
! GAGGTAGAAATTTTGTGCTA+TGG + Chr8:44189001-44189020 MsG0880044546.01.T01:intron 35.0%
! TGTGCTATGGTTGTTTCATA+TGG + Chr8:44189014-44189033 MsG0880044546.01.T01:intron 35.0%
! TTAAAAACCACTCTGGTTCA+AGG - Chr8:44189877-44189896 None:intergenic 35.0%
AAGTCAGAAATCAAGTCACG+AGG + Chr8:44188982-44189001 MsG0880044546.01.T01:intron 40.0%
AGGAATACATCGAAGTCTTG+GGG + Chr8:44188846-44188865 MsG0880044546.01.T01:CDS 40.0%
CAGGAATACATCGAAGTCTT+GGG + Chr8:44188845-44188864 MsG0880044546.01.T01:CDS 40.0%
CCGACAAATTATGACAGAGT+TGG + Chr8:44189812-44189831 MsG0880044546.01.T01:CDS 40.0%
GAATAAATGCAACCTGCTCT+GGG - Chr8:44189650-44189669 None:intergenic 40.0%
GTTTATACCTTGAACCAGAG+TGG + Chr8:44189867-44189886 MsG0880044546.01.T01:CDS 40.0%
TAATGTGACTCAGTTTGACG+TGG + Chr8:44189409-44189428 MsG0880044546.01.T01:intron 40.0%
TCAGGAATACATCGAAGTCT+TGG + Chr8:44188844-44188863 MsG0880044546.01.T01:CDS 40.0%
TCTAAATGGTCATGACCTTG+TGG + Chr8:44189682-44189701 MsG0880044546.01.T01:intron 40.0%
TGAATAAATGCAACCTGCTC+TGG - Chr8:44189651-44189670 None:intergenic 40.0%
!!! GCTCCTAGGTCCTTTTTTAT+TGG + Chr8:44189447-44189466 MsG0880044546.01.T01:intron 40.0%
ACTACTGGAAAATCGCTCCT+AGG + Chr8:44189433-44189452 MsG0880044546.01.T01:intron 45.0%
AGATTGACACTAGCCCATCT+TGG - Chr8:44188799-44188818 None:intergenic 45.0%
GATTTGCAGAGGACCAAGAT+GGG + Chr8:44188783-44188802 MsG0880044546.01.T01:CDS 45.0%
TCAGTTTGACGTGGAACTAC+TGG + Chr8:44189418-44189437 MsG0880044546.01.T01:intron 45.0%
TGCTGGGATGATAGTCTACT+TGG + Chr8:44189335-44189354 MsG0880044546.01.T01:intron 45.0%
! GTAGGTTCGTTCTGCTACTT+TGG + Chr8:44189614-44189633 MsG0880044546.01.T01:intron 45.0%
! TGATTTGCAGAGGACCAAGA+TGG + Chr8:44188782-44188801 MsG0880044546.01.T01:CDS 45.0%
!! AGATGGGCTAGTGTCAATCT+TGG + Chr8:44188799-44188818 MsG0880044546.01.T01:CDS 45.0%
!!! ATAATTGATTTAAATTTAAA+AGG - Chr8:44189389-44189408 None:intergenic 5.0%
!! GTTCTGCTACTTTGGACACC+TGG + Chr8:44189622-44189641 MsG0880044546.01.T01:CDS 50.0%
!! TGCAACCTGCTCTGGGAGCC+AGG - Chr8:44189643-44189662 None:intergenic 65.0%
GGACACCTGGCTCCCAGAGC+AGG + Chr8:44189635-44189654 MsG0880044546.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 44188772 44189931 44188772 ID=MsG0880044546.01;Name=MsG0880044546.01
Chr8 mRNA 44188772 44189931 44188772 ID=MsG0880044546.01.T01;Parent=MsG0880044546.01;Name=MsG0880044546.01.T01;_AED=0.48;_eAED=0.51;_QI=0|1|0.33|1|1|1|3|0|109
Chr8 exon 44188772 44188882 44188772 ID=MsG0880044546.01.T01:exon:17779;Parent=MsG0880044546.01.T01
Chr8 exon 44189618 44189672 44189618 ID=MsG0880044546.01.T01:exon:17780;Parent=MsG0880044546.01.T01
Chr8 exon 44189768 44189931 44189768 ID=MsG0880044546.01.T01:exon:17781;Parent=MsG0880044546.01.T01
Chr8 CDS 44188772 44188882 44188772 ID=MsG0880044546.01.T01:cds;Parent=MsG0880044546.01.T01
Chr8 CDS 44189618 44189672 44189618 ID=MsG0880044546.01.T01:cds;Parent=MsG0880044546.01.T01
Chr8 CDS 44189768 44189931 44189768 ID=MsG0880044546.01.T01:cds;Parent=MsG0880044546.01.T01
Gene Sequence

>MsG0880044546.01.T01

ATGGAAATTTTGATTTGCAGAGGACCAAGATGGGCTAGTGTCAATCTTGGCATTTTTATTTGCATGAGTTGTTCAGGAATACATCGAAGTCTTGGGGTACATATTTCAAAGGTTCGTTCTGCTACTTTGGACACCTGGCTCCCAGAGCAGGTTGCATTTATTCAATCTATGGGAAATGAGAGAGCAAATAGTTACTGGGAAGCAGAATTACCGACAAATTATGACAGAGTTGGCATTGAAAACTTCATCCGTGCAAAGTATGTTAGTTTATACCTTGAACCAGAGTGGTTTTTAAAAAAAATAAAATATAATAGTCTTTGTACAGTTTGA

Protein sequence

>MsG0880044546.01.T01

MEILICRGPRWASVNLGIFICMSCSGIHRSLGVHISKVRSATLDTWLPEQVAFIQSMGNERANSYWEAELPTNYDRVGIENFIRAKYVSLYLEPEWFLKKIKYNSLCTV*