Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040008.01.T01 | XP_003624458.1 | 97.436 | 390 | 10 | 0 | 1 | 390 | 1 | 390 | 0 | 794 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001795.01 | MsG0780040008.01 | 0.814064 | 1.863572e-51 | 1.237827e-48 |
| MsG0380013071.01 | MsG0780040008.01 | 0.837147 | 6.170032e-57 | 7.943244e-54 |
| MsG0380016315.01 | MsG0780040008.01 | -0.816301 | 5.935490e-52 | 4.190236e-49 |
| MsG0380017254.01 | MsG0780040008.01 | 0.853514 | 2.272755e-61 | 4.923449e-58 |
| MsG0480021102.01 | MsG0780040008.01 | 0.808959 | 2.396585e-50 | 1.389169e-47 |
| MsG0480021249.01 | MsG0780040008.01 | 0.846888 | 1.636669e-59 | 2.857909e-56 |
| MsG0480021251.01 | MsG0780040008.01 | 0.815814 | 7.621237e-52 | 5.308934e-49 |
| MsG0580025052.01 | MsG0780040008.01 | -0.885078 | 1.108041e-71 | 7.498574e-68 |
| MsG0580029642.01 | MsG0780040008.01 | 0.809880 | 1.520495e-50 | 9.032344e-48 |
| MsG0780040008.01 | MsG0780040038.01 | 0.925350 | 2.066250e-90 | 9.179803e-86 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040008.01.T01 | MTR_7g083540 | 97.436 | 390 | 10 | 0 | 1 | 390 | 1 | 390 | 0.0 | 794 |
| MsG0780040008.01.T01 | MTR_7g108150 | 47.445 | 137 | 54 | 4 | 4 | 129 | 19 | 148 | 9.71e-31 | 121 |
| MsG0780040008.01.T01 | MTR_7g108150 | 55.556 | 63 | 26 | 1 | 322 | 382 | 307 | 369 | 1.07e-13 | 72.4 |
| MsG0780040008.01.T01 | MTR_5g069480 | 43.396 | 106 | 54 | 2 | 5 | 110 | 5 | 104 | 1.21e-23 | 100 |
| MsG0780040008.01.T01 | MTR_5g069480 | 42.727 | 110 | 57 | 2 | 1 | 110 | 1 | 104 | 1.70e-23 | 101 |
| MsG0780040008.01.T01 | MTR_3g082630 | 39.568 | 139 | 76 | 3 | 1 | 138 | 1 | 132 | 3.42e-23 | 100 |
| MsG0780040008.01.T01 | MTR_3g082630 | 56.061 | 66 | 29 | 0 | 318 | 383 | 331 | 396 | 1.53e-14 | 75.1 |
| MsG0780040008.01.T01 | MTR_2g088900 | 43.119 | 109 | 52 | 2 | 1 | 108 | 97 | 196 | 2.18e-19 | 90.1 |
| MsG0780040008.01.T01 | MTR_2g088900 | 26.065 | 399 | 251 | 12 | 2 | 385 | 141 | 510 | 4.95e-15 | 77.0 |
| MsG0780040008.01.T01 | MTR_1g109350 | 34.188 | 117 | 68 | 2 | 1 | 117 | 1 | 108 | 3.00e-17 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040008.01.T01 | AT2G33500 | 35.225 | 423 | 198 | 11 | 5 | 385 | 12 | 400 | 5.06e-64 | 210 |
| MsG0780040008.01.T01 | AT2G33500 | 36.298 | 416 | 204 | 11 | 5 | 385 | 12 | 401 | 6.85e-64 | 210 |
| MsG0780040008.01.T01 | AT1G28050 | 53.509 | 114 | 47 | 1 | 4 | 117 | 8 | 115 | 1.22e-36 | 138 |
| MsG0780040008.01.T01 | AT1G28050 | 39.785 | 186 | 87 | 6 | 215 | 385 | 254 | 429 | 4.37e-22 | 97.8 |
| MsG0780040008.01.T01 | AT2G47890 | 48.361 | 122 | 55 | 2 | 5 | 126 | 13 | 126 | 9.11e-29 | 114 |
| MsG0780040008.01.T01 | AT2G47890 | 48.361 | 122 | 55 | 2 | 5 | 126 | 13 | 126 | 1.01e-28 | 112 |
| MsG0780040008.01.T01 | AT2G47890 | 48.361 | 122 | 55 | 2 | 5 | 126 | 13 | 126 | 1.05e-28 | 114 |
| MsG0780040008.01.T01 | AT2G47890 | 62.745 | 51 | 19 | 0 | 335 | 385 | 281 | 331 | 1.90e-13 | 71.2 |
| MsG0780040008.01.T01 | AT5G48250 | 41.322 | 121 | 67 | 1 | 1 | 121 | 1 | 117 | 2.27e-25 | 106 |
| MsG0780040008.01.T01 | AT5G48250 | 61.224 | 49 | 19 | 0 | 337 | 385 | 312 | 360 | 6.83e-13 | 69.7 |
Find 98 sgRNAs with CRISPR-Local
Find 298 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAGAGTTTCGCGGAGTC+TGG | 0.399009 | 7:+71901092 | None:intergenic |
| AAATTAAGCAAAGAAGTAAA+CGG | 0.500811 | 7:+71900606 | None:intergenic |
| AACCGGTGAGACCTTCTATA+CGG | 0.471948 | 7:+71900865 | None:intergenic |
| AACCTGGGGAGGCTGATACG+TGG | 0.646766 | 7:+71900500 | None:intergenic |
| AAGAGAAATAGTAATGATTA+TGG | 0.284505 | 7:-71900558 | MsG0780040008.01.T01:CDS |
| AAGAGCACACAAGGAATAGA+GGG | 0.577298 | 7:-71897582 | MsG0780040008.01.T01:CDS |
| AAGGGAAATGCCATGGGATG+AGG | 0.502225 | 7:+71897184 | None:intergenic |
| AATATGAATTGCTCAACATT+TGG | 0.400714 | 7:-71898895 | MsG0780040008.01.T01:CDS |
| AATGATTATGGAAATGAATC+TGG | 0.399310 | 7:-71900546 | MsG0780040008.01.T01:CDS |
| ACAAATTAACAAACCTGGGG+AGG | 0.611687 | 7:+71900489 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACACAAATTAACAAACCTG+GGG | + | Chr7:71897833-71897852 | None:intergenic | 30.0% | |
| AAGGTTTAGTTACATTACCA+AGG | - | Chr7:71898342-71898361 | MsG0780040008.01.T01:intron | 30.0% | |
| ACATTGAATCAAAGCAATGT+AGG | + | Chr7:71900281-71900300 | None:intergenic | 30.0% | |
| AGAAAATAGGTCAGTTATTC+CGG | + | Chr7:71899916-71899935 | None:intergenic | 30.0% | |
| AGGAAGATAAGTAGTTGAAA+AGG | - | Chr7:71898006-71898025 | MsG0780040008.01.T01:intron | 30.0% | |
| ATCTCTGTGCTTAATGTATT+AGG | - | Chr7:71899670-71899689 | MsG0780040008.01.T01:intron | 30.0% | |
| ATGCAAGATAACAACACATT+AGG | + | Chr7:71899011-71899030 | None:intergenic | 30.0% | |
| ATGTGATTTAAATGGTTGAG+TGG | - | Chr7:71899786-71899805 | MsG0780040008.01.T01:intron | 30.0% | |
| ATTGAATCCATAACATACAG+TGG | - | Chr7:71900631-71900650 | MsG0780040008.01.T01:CDS | 30.0% | |
| CAAAGTAAGTCAATTAAACC+GGG | - | Chr7:71900098-71900117 | MsG0780040008.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 71897068 | 71901270 | 71897068 | ID=MsG0780040008.01;Name=MsG0780040008.01 |
| Chr7 | mRNA | 71897068 | 71901270 | 71897068 | ID=MsG0780040008.01.T01;Parent=MsG0780040008.01;Name=MsG0780040008.01.T01;_AED=0.36;_eAED=0.36;_QI=102|1|1|1|1|1|4|154|390 |
| Chr7 | exon | 71897068 | 71897327 | 71897068 | ID=MsG0780040008.01.T01:exon:13423;Parent=MsG0780040008.01.T01 |
| Chr7 | exon | 71897541 | 71897800 | 71897541 | ID=MsG0780040008.01.T01:exon:13422;Parent=MsG0780040008.01.T01 |
| Chr7 | exon | 71898873 | 71899013 | 71898873 | ID=MsG0780040008.01.T01:exon:13421;Parent=MsG0780040008.01.T01 |
| Chr7 | exon | 71900503 | 71901270 | 71900503 | ID=MsG0780040008.01.T01:exon:13420;Parent=MsG0780040008.01.T01 |
| Chr7 | five_prime_UTR | 71901169 | 71901270 | 71901169 | ID=MsG0780040008.01.T01:five_prime_utr;Parent=MsG0780040008.01.T01 |
| Chr7 | CDS | 71900503 | 71901168 | 71900503 | ID=MsG0780040008.01.T01:cds;Parent=MsG0780040008.01.T01 |
| Chr7 | CDS | 71898873 | 71899013 | 71898873 | ID=MsG0780040008.01.T01:cds;Parent=MsG0780040008.01.T01 |
| Chr7 | CDS | 71897541 | 71897800 | 71897541 | ID=MsG0780040008.01.T01:cds;Parent=MsG0780040008.01.T01 |
| Chr7 | CDS | 71897222 | 71897327 | 71897222 | ID=MsG0780040008.01.T01:cds;Parent=MsG0780040008.01.T01 |
| Chr7 | three_prime_UTR | 71897068 | 71897221 | 71897068 | ID=MsG0780040008.01.T01:three_prime_utr;Parent=MsG0780040008.01.T01 |
| Gene Sequence |
>MsG0780040008.01.T01 ATGTCGTTTCCGTGTGACTATTGCGACACAAGAAACGCAGTGCTATATTGCAAACCAGACTCCGCGAAACTCTGTTTAGTTTGCGACCAGCACGTGCACTCCGCAAACGCACTCGCACTCAAGCACGTGCGTTTCCAAATTTGCCAAAACTGCAAAAACGACGCCGCTTCAGTTCGTTGCTTCACCGAAAACCTAGTTCAATGTCACCGCTGCGATTGGAACTCTCACGGCGGCGACGACGACAACCCCACCTCTTCATCTTTCCACCACCACAACCGCCGCCGTATAGAAGGTCTCACCGGTTGTCCTTCCGTTCACGAGATCGTTTCTACTTTGGGACTTGATTTGAAGCCGAATGACTCCGTTTTCGTAGCGGAATTTGAAGGTCCGGTGGTTCCGGTTGTGAAGAATAGGGATGAGGTGTATGAACAGGTGGTGGAAGTTGCGAAGAGGAAGAGGAATTTGGAGGAAGATGAAAATGAACTGAGATTCAATGATTGTTGTAATGATGTTGATGATTTGTTGTTGCTTCAGCAAACTCCGTTTACTTCTTTGCTTAATTTTTCGTCTGAATTTGATGTTGGTGCTAAGAGAAATAGTAATGATTATGGAAATGAATCTGGTCTTCTTCTTTGGGATCGTAATCCCACGTATCAGCCTCCCCAGGTCTGGGATTTTCAATTACAGAAATCAAGAGATATGACATATGATGGTGTGGAAAATGCTTCCTTATCGATTCCAAAATCATTGCAAGATGTGTACAATATGAATTGCTCAACATTTGGTGATGATATTCTATCAAGAAATAATCAATCGGATCAGTCATCATCAAGCCATGTGAAGAAGAAAGTAGAAAGCAACAAGAAAACTAGAGATGGGTTGTCTTCTGAGTCCAAATTGATTGAATCCATAACATACAGTGGCGCCGACAGTGTACCAGTTATGGAACATCTTTTGAGTGGCAGTGAAAATGTTAGCAACATAAATGCCAAGATTGGCTTGGAAGAGCACACAAGGAATAGAGGGGATGCCATGTTACGGTACAAGGAGAAGAAGAAAACTCGAAGGTTTGACAAGCACATTCGCTATGAATCAAGGAAGGCTAGGGCTGATACGAGAAAGAGAGTGAGAGGGCGATTTGTTAAGGCAACTGATGATATTCAAGAAGGATGA |
| Protein sequence |
>MsG0780040008.01.T01 MSFPCDYCDTRNAVLYCKPDSAKLCLVCDQHVHSANALALKHVRFQICQNCKNDAASVRCFTENLVQCHRCDWNSHGGDDDNPTSSSFHHHNRRRIEGLTGCPSVHEIVSTLGLDLKPNDSVFVAEFEGPVVPVVKNRDEVYEQVVEVAKRKRNLEEDENELRFNDCCNDVDDLLLLQQTPFTSLLNFSSEFDVGAKRNSNDYGNESGLLLWDRNPTYQPPQVWDFQLQKSRDMTYDGVENASLSIPKSLQDVYNMNCSTFGDDILSRNNQSDQSSSSHVKKKVESNKKTRDGLSSESKLIESITYSGADSVPVMEHLLSGSENVSNINAKIGLEEHTRNRGDAMLRYKEKKKTRRFDKHIRYESRKARADTRKRVRGRFVKATDDIQEG* |
