AlfalfaGEDB | MsG0580029642.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029642.01

KEGG: -
GO: GO:0003676 GO:0005524
PFAM: PF00270 PF00271

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029642.01.T01	XP_003617301.2	98.477	591	8	1	1	591	1	590	0	1159

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029642.01.T01	Q9LUW5	66.078	510	149	4	1	486	1	510	0	660

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029642.01.T01	G7KCY5	98.477	591	8	1	1	591	1	590	0.0	1159

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001213.01	MsG0580029642.01	0.807199	5.680456e-50	3.144332e-47
MsG0180001795.01	MsG0580029642.01	0.845825	3.188532e-59	5.381151e-56
MsG0180003128.01	MsG0580029642.01	0.820495	6.656286e-53	5.275055e-50
MsG0280010942.01	MsG0580029642.01	0.821836	3.266840e-53	2.687874e-50
MsG0280011120.01	MsG0580029642.01	-0.827660	1.384064e-54	1.344750e-51
MsG0380012310.01	MsG0580029642.01	0.834414	3.038821e-56	3.603805e-53
MsG0380012566.01	MsG0580029642.01	0.813590	2.369512e-51	1.553840e-48
MsG0380013071.01	MsG0580029642.01	0.805137	1.543204e-49	8.101686e-47
MsG0380016315.01	MsG0580029642.01	-0.803711	3.059708e-49	1.548328e-46
MsG0380017254.01	MsG0580029642.01	0.853008	3.174595e-61	6.760380e-58

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PPI

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Gene1	Gene2	Type
MsG0580029403.01	MsG0580029642.01	PPI
MsG0580029642.01	MsG0580029399.01	PPI
MsG0580029642.01	MsG0580029400.01	PPI
MsG0580029642.01	MsG0580029413.01	PPI
MsG0580029642.01	MsG0280007117.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029642.01.T01	MTR_5g090120	98.477	591	8	1	1	591	1	590	0.0	1159
MsG0580029642.01.T01	MTR_3g449250	77.283	427	94	1	40	463	46	472	0.0	677
MsG0580029642.01.T01	MTR_3g449250	77.283	427	94	1	40	463	46	472	0.0	677
MsG0580029642.01.T01	MTR_1g102410	42.640	394	197	6	82	450	45	434	1.16e-94	303
MsG0580029642.01.T01	MTR_6g056080	43.229	384	203	8	77	450	99	477	5.04e-90	292
MsG0580029642.01.T01	MTR_2g100230	39.542	349	202	5	91	434	106	450	5.08e-80	261
MsG0580029642.01.T01	MTR_1g054630	35.477	451	274	8	83	517	156	605	2.73e-76	254
MsG0580029642.01.T01	MTR_4g105100	38.642	383	215	11	90	463	214	585	2.01e-75	252
MsG0580029642.01.T01	MTR_3g467550	39.785	372	204	7	69	429	81	443	6.87e-74	246
MsG0580029642.01.T01	MTR_6g005550	37.322	351	210	5	83	428	167	512	4.77e-73	248

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029642.01.T01	AT3G22330	66.078	510	149	4	1	486	1	510	0.0	660
MsG0580029642.01.T01	AT3G22310	60.545	550	138	5	1	511	1	510	0.0	638
MsG0580029642.01.T01	AT5G26742	65.508	374	125	1	78	447	100	473	1.24e-168	498
MsG0580029642.01.T01	AT5G26742	65.508	374	125	1	78	447	100	473	1.28e-168	498
MsG0580029642.01.T01	AT5G26742	66.279	344	112	1	108	447	37	380	1.12e-156	464
MsG0580029642.01.T01	AT5G62190	45.503	378	193	6	82	450	98	471	6.26e-94	302
MsG0580029642.01.T01	AT1G55150	39.467	413	220	12	78	467	90	495	1.55e-83	270
MsG0580029642.01.T01	AT1G55150	39.467	413	220	12	78	467	115	520	1.40e-82	268
MsG0580029642.01.T01	AT5G63120	38.873	355	208	5	90	439	175	525	1.92e-79	262
MsG0580029642.01.T01	AT5G14610	37.822	349	204	6	86	429	167	507	6.54e-75	251

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Find 171 sgRNAs with CRISPR-Local

Find 354 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAATATCTCTGAATTAT+TGG	0.139299	5:-100166555	None:intergenic
AAGAGAATCTCGGCGGCGGC+GGG	0.401354	5:-100163249	None:intergenic
AAGATCAATCCAGGACTCTT+AGG	0.323614	5:+100166639	MsG0580029642.01.T01:CDS
AATCTCGGCGGCGGCGGGGA+TGG	0.397597	5:-100163244	None:intergenic
AATTACTGTAGCCTCCAGAA+CGG	0.666725	5:-100167514	None:intergenic
AATTGAACGCAAACCTGAAT+GGG	0.465119	5:-100163507	None:intergenic
ACAAATATCTCTGAATTATT+GGG	0.364531	5:-100166554	None:intergenic
ACACAGATAGTATCCAAATT+AGG	0.347866	5:-100164297	None:intergenic
ACACTTGCTGGCTTCAGAAA+CGG	0.545670	5:+100165404	MsG0580029642.01.T01:CDS
ACGGAGCTCAGTGGCGGCGG+TGG	0.427658	5:-100163277	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATATTTAGTCCCGTCTT+AGG	+	Chr5:100165603-100165622	MsG0580029642.01.T01:intron	30.0%
AATAAGCTCTTTATCCAAAC+AGG	-	Chr5:100163872-100163891	None:intergenic	30.0%
ACACAGATAGTATCCAAATT+AGG	-	Chr5:100164300-100164319	None:intergenic	30.0%
ACCTATTAACTTTCGTAGTA+AGG	+	Chr5:100164749-100164768	MsG0580029642.01.T01:intron	30.0%
AGCAAAACTTACAAGATCAA+TGG	-	Chr5:100164616-100164635	None:intergenic	30.0%
AGGTAATAAAGATTGACAGA+TGG	-	Chr5:100166727-100166746	None:intergenic	30.0%
ATAACTTTACGATGTCAATG+GGG	-	Chr5:100165521-100165540	None:intergenic	30.0%
ATAAGCTCTTTATCCAAACA+GGG	-	Chr5:100163871-100163890	None:intergenic	30.0%
ATGGAAATGGATAATATGCA+GGG	-	Chr5:100166708-100166727	None:intergenic	30.0%
ATTAGAATCTTGGATGTAAG+GGG	+	Chr5:100166227-100166246	MsG0580029642.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	100163195	100167820	100163195	ID=MsG0580029642.01;Name=MsG0580029642.01
Chr5	mRNA	100163195	100167820	100163195	ID=MsG0580029642.01.T01;Parent=MsG0580029642.01;Name=MsG0580029642.01.T01;_AED=0.33;_eAED=0.33;_QI=0\|0\|0\|1\|1\|1\|7\|0\|591
Chr5	exon	100163195	100163515	100163195	ID=MsG0580029642.01.T01:exon:36425;Parent=MsG0580029642.01.T01
Chr5	exon	100163670	100163797	100163670	ID=MsG0580029642.01.T01:exon:36426;Parent=MsG0580029642.01.T01
Chr5	exon	100164212	100164623	100164212	ID=MsG0580029642.01.T01:exon:36427;Parent=MsG0580029642.01.T01
Chr5	exon	100165062	100165160	100165062	ID=MsG0580029642.01.T01:exon:36428;Parent=MsG0580029642.01.T01
Chr5	exon	100165269	100165493	100165269	ID=MsG0580029642.01.T01:exon:36429;Parent=MsG0580029642.01.T01
Chr5	exon	100166536	100166697	100166536	ID=MsG0580029642.01.T01:exon:36430;Parent=MsG0580029642.01.T01
Chr5	exon	100167392	100167820	100167392	ID=MsG0580029642.01.T01:exon:36431;Parent=MsG0580029642.01.T01
Chr5	CDS	100163195	100163515	100163195	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01
Chr5	CDS	100163670	100163797	100163670	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01
Chr5	CDS	100164212	100164623	100164212	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01
Chr5	CDS	100165062	100165160	100165062	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01
Chr5	CDS	100165269	100165493	100165269	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01
Chr5	CDS	100166536	100166697	100166536	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01
Chr5	CDS	100167392	100167820	100167392	ID=MsG0580029642.01.T01:cds;Parent=MsG0580029642.01.T01

Gene Sequence

>MsG0580029642.01.T01

ATGATTACCACTATCCTCAGACGCGCTTCTTCAACCCTCTCACGGCGAACCATCCCCGCCGCCGCCGAGATTCTCTTCTCCACCACCGCCGCCACTGAGCTCCGTCACCTCACAGCAAGATCCTTTCACTCTAAATCACAACCCTTACTATTCCGCGCTTCTTCTGCTTCTCGCGCTGGTTACGCCGCCGAAGCTTTTCGTTTTGAAGAACCTTCGAAATCGAATAGCGATGAGGGTCTTGAGATCGCGAAGCTTGGGATTTCTCAGGATATTGTCGCTGCTTTGGAGAAGAAAGGAATCACCAAGCTTTTTCCCATTCAGAGGGCTGTGCTGGAACCTGCTATGCAAGGTCGTGATATGATTGGTCGAGCAAGAACAGGAACTGGAAAAACTCTTGCTTTTGGGATACCCATCATGGATAAGATCATTCAGTTTAATGCTAAACATGGGCGAGGAAAGGATCCTCTGGCTTTGGTTTTGGCGCCGACTAGAGAACTTGCGAAGCAGGTTGAGAAGGAATTCTACGAGTCGGCGCCTAATTTGGATACTATCTGTGTTTATGGGGGTACCCCGATCTCTCAACAGATGAGGCAGCTTGATTATGGTGTTGATATAGCGGTTGGTACACCTGGACGAATTATTGATCTTCTTAATAGAGGTGCTCTAAATTTGAAAGAAGTCCAGTTTGTTATTCTTGACGAAGCCGATCAGATGCTTCAAGTTGGGTTCCAGGAAGACGTGGAGAAGATATTGGAGAGATTACCCACTGAGAGACAAACTCTCATGTTCTCTGCAACAATGCCAACTTGGATAAAGCAGTTAACACGCAATTACCTCAAAAATCCTCTAACCATTGATCTTGTTGGAGATTCTGATCAGAAGCTGGCAGATGGAATTTCACTGTACTCGATTCTATCTGATGCGTATGTCAAAGCAGGAATTATTGTTCCTCTAATTAAAGAACATGCAAAAGGAGGAAAGTGTATTGTTTTCACCCAAACAAAACGTGACGCTGATCGAATATCACATGGCATGTCAAAAAGTATTCCGTGTGAGGCATTGCATGGAGATATATCACAAGTCCAGAGAGAAAGGACACTTGCTGGCTTCAGAAACGGCCATTTTAATGTTTTAGTGGCAACTGATGTTGCTTCACGTGGGCTCGATATACCTAATGTTGATCTTGTGATTCATTATGAACTTCCCAATAATTCAGAGATATTTGTTCACCGATCTGGACGAACTGGTCGTGCTGGTAAGAAAGGAACTGCTATTCTTGTTTATACACAAGATCAATCCAGGACTCTTAGGACTATTGAGCGCGATGTAGGCTGTAAATTTTCAGAGTTACCAAAAATTGCCGTTGACACTGCATCACTGGGCGGATTTAGTGGTGGCGGCAGATTTGGTGGTGGCGGTGGTGGATTTGGTGGTGGGGGTGGCGGCCGATCTGGTTCTTTTGTTGGTGGCCGTTCTGGAGGCTACAGTAATTCAGGCGGGTTTTCTGGTTCAAGACAATCTGGAGGGGGGTTTTCTGGTGGATCAAGCTCATATGGTGAAAATAGATATGGCGGATCAAGTTCTGGTCGTTTTGGCTCCTTTGGTTCCGGTGATTCAGGCAGTAGATCTGGTGGATCCTCTGGTGGATTCAGCAAACAAGGTGGTTTTGGTGGATTCGGTGGTGGTTCGGATCGCTCTGGTGGATTCGGTGGTGGTTTTGGAGATTTTCGGTCGGGTAAACCTGGCGCATTTGGTGATCGTCGTGGCAGGGATTAG

Protein sequence

>MsG0580029642.01.T01

MITTILRRASSTLSRRTIPAAAEILFSTTAATELRHLTARSFHSKSQPLLFRASSASRAGYAAEAFRFEEPSKSNSDEGLEIAKLGISQDIVAALEKKGITKLFPIQRAVLEPAMQGRDMIGRARTGTGKTLAFGIPIMDKIIQFNAKHGRGKDPLALVLAPTRELAKQVEKEFYESAPNLDTICVYGGTPISQQMRQLDYGVDIAVGTPGRIIDLLNRGALNLKEVQFVILDEADQMLQVGFQEDVEKILERLPTERQTLMFSATMPTWIKQLTRNYLKNPLTIDLVGDSDQKLADGISLYSILSDAYVKAGIIVPLIKEHAKGGKCIVFTQTKRDADRISHGMSKSIPCEALHGDISQVQRERTLAGFRNGHFNVLVATDVASRGLDIPNVDLVIHYELPNNSEIFVHRSGRTGRAGKKGTAILVYTQDQSRTLRTIERDVGCKFSELPKIAVDTASLGGFSGGGRFGGGGGGFGGGGGGRSGSFVGGRSGGYSNSGGFSGSRQSGGGFSGGSSSYGENRYGGSSSGRFGSFGSGDSGSRSGGSSGGFSKQGGFGGFGGGSDRSGGFGGGFGDFRSGKPGAFGDRRGRD*