AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040482.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040482.01.T01 MTR_7g091390 97.619 84 2 0 8 91 227 310 1.69e-53 172
MsG0780040482.01.T01 MTR_7g091390 97.619 84 2 0 8 91 315 398 6.24e-53 173
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040482.01.T01 AT2G41710 55.294 85 28 3 8 91 331 406 2.39e-20 87.0
MsG0780040482.01.T01 AT2G41710 55.294 85 28 3 8 91 366 441 2.50e-20 87.0
MsG0780040482.01.T01 AT2G41710 55.294 85 28 3 8 91 336 411 2.54e-20 86.7
MsG0780040482.01.T01 AT2G41710 55.294 85 28 3 8 91 331 406 2.96e-20 86.7

Find 41 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGTACAAGCTCAGCTTTC+AGG 0.147623 7:+78233920 None:intergenic
CTCATTTAACAATGCTAAAA+AGG 0.206842 7:-78235023 MsG0780040482.01.T01:CDS
CACATTAATCCAAATGTTCT+TGG 0.247919 7:+78234037 None:intergenic
GCTTTCTTCAATTAGAAATT+CGG 0.325409 7:+78233949 None:intergenic
TTCACCTTACCAAGAACATT+TGG 0.367815 7:-78234046 MsG0780040482.01.T01:CDS
AAGCTCAGCTTTCAGGCTCT+TGG 0.370253 7:+78233927 None:intergenic
GTTTCAGCCACGGGAGTGAT+TGG 0.381045 7:-78233976 MsG0780040482.01.T01:CDS
CATTGGATCAACAGTGTTGT+AGG 0.410420 7:+78235171 None:intergenic
GAACATTTGGATTAATGTGC+AGG 0.411774 7:-78234033 MsG0780040482.01.T01:CDS
AAACATAGTGTAGGTTGAAT+CGG 0.419840 7:+78233995 None:intergenic
CAATGGTAGATCCTGCTCTC+TGG 0.448245 7:-78235153 MsG0780040482.01.T01:CDS
TTGCTGCCATCATGATTTGA+AGG 0.466238 7:+78235292 None:intergenic
TAATCCAAATGTTCTTGGTA+AGG 0.474955 7:+78234042 None:intergenic
ATGAGAGATGTCCAGAGAGC+AGG 0.478776 7:+78235142 None:intergenic
AACATAGTGTAGGTTGAATC+GGG 0.509559 7:+78233996 None:intergenic
CTCAGATGTACGAGAAAGGC+CGG 0.518845 7:+78235105 None:intergenic
AGAAAGGCCGGCAGGAAGCA+TGG 0.520929 7:+78235117 None:intergenic
CTCATTCCCATGCTTCCTGC+CGG 0.523635 7:-78235124 MsG0780040482.01.T01:CDS
GATCGACTTGACATTGCAAT+GGG 0.526683 7:-78235268 MsG0780040482.01.T01:CDS
CAGACAAGAATGATGTCAAT+GGG 0.541457 7:+78235209 None:intergenic
CTTTCTCGTACATCTGAGGT+TGG 0.548824 7:-78235100 MsG0780040482.01.T01:CDS
CGGCCTTTCTCGTACATCTG+AGG 0.553206 7:-78235104 MsG0780040482.01.T01:CDS
CAACCTCAGATGTACGAGAA+AGG 0.556043 7:+78235101 None:intergenic
GATGTACGAGAAAGGCCGGC+AGG 0.557024 7:+78235109 None:intergenic
CAGAGAGCAGGATCTACCAT+TGG 0.557981 7:+78235154 None:intergenic
CTACACTATGTTTCAGCCAC+GGG 0.558537 7:-78233985 MsG0780040482.01.T01:CDS
GAAAGGCCGGCAGGAAGCAT+GGG 0.562709 7:+78235118 None:intergenic
TGATCGACTTGACATTGCAA+TGG 0.573444 7:-78235269 MsG0780040482.01.T01:CDS
GCAGACAAGAATGATGTCAA+TGG 0.579355 7:+78235208 None:intergenic
CTACAACACTGTTGATCCAA+TGG 0.582269 7:-78235170 MsG0780040482.01.T01:CDS
GAGAAACCTTCAAATCATGA+TGG 0.583795 7:-78235298 MsG0780040482.01.T01:CDS
TAGTCAATGAGTTGGATCGT+GGG 0.590774 7:-78235330 MsG0780040482.01.T01:CDS
ATAGTCAATGAGTTGGATCG+TGG 0.596990 7:-78235331 MsG0780040482.01.T01:CDS
CAATGAGTTGGATCGTGGGA+AGG 0.597269 7:-78235326 MsG0780040482.01.T01:CDS
GACATTGCAATGGGAATGAG+TGG 0.603558 7:-78235259 MsG0780040482.01.T01:CDS
CCTACACTATGTTTCAGCCA+CGG 0.629098 7:-78233986 MsG0780040482.01.T01:CDS
CGGATGTCCAATCACTCCCG+TGG 0.636413 7:+78233969 None:intergenic
GTGTTGTAGGCTGTCAAAAG+TGG 0.663016 7:+78235184 None:intergenic
CCGTGGCTGAAACATAGTGT+AGG 0.666827 7:+78233986 None:intergenic
ACATTGCAATGGGAATGAGT+GGG 0.685010 7:-78235258 MsG0780040482.01.T01:CDS
CATTGCAATGGGAATGAGTG+GGG 0.722588 7:-78235257 MsG0780040482.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAATTGGTTTATTAT+GGG - Chr7:78234596-78234615 MsG0780040482.01.T01:intron 10.0%
!! ACTGTAATTTGAAAAATTAT+TGG + Chr7:78234986-78235005 None:intergenic 15.0%
!!! AATAAACCAATTTAAAATGT+TGG + Chr7:78234594-78234613 None:intergenic 15.0%
!!! AATATAATTAATGCAGTTTT+AGG - Chr7:78234847-78234866 MsG0780040482.01.T01:intron 15.0%
!!! CATTTTAAATTGGTTTATTA+TGG - Chr7:78234595-78234614 MsG0780040482.01.T01:intron 15.0%
!!! TTTTTCAAATTACAGTTTTA+AGG - Chr7:78234990-78235009 MsG0780040482.01.T01:intron 15.0%
!! AAATATGTGAGATATGATTA+TGG + Chr7:78234896-78234915 None:intergenic 20.0%
!! ACTAGCATCTATAAAATTAA+AGG + Chr7:78234664-78234683 None:intergenic 20.0%
!!! AAAAAGCATTAAATGAAAGT+AGG + Chr7:78234342-78234361 None:intergenic 20.0%
!!! AGTATTTTACTTTATTCACA+AGG - Chr7:78234218-78234237 MsG0780040482.01.T01:intron 20.0%
!!! TATTTGATAACATTCACATT+AGG + Chr7:78234924-78234943 None:intergenic 20.0%
!!! TATTTTTACTTTTATGAGTC+TGG - Chr7:78234473-78234492 MsG0780040482.01.T01:intron 20.0%
!!! TATTTTTTCTTAAGTACTTC+TGG - Chr7:78234795-78234814 MsG0780040482.01.T01:intron 20.0%
!!! TTTAATTTTATAGATGCTAG+TGG - Chr7:78234663-78234682 MsG0780040482.01.T01:intron 20.0%
! AACAAAAATAGTCAATGAGT+TGG - Chr7:78233945-78233964 MsG0780040482.01.T01:CDS 25.0%
! CTCATTTAACAATGCTAAAA+AGG - Chr7:78234260-78234279 MsG0780040482.01.T01:intron 25.0%
! GCTTTCTTCAATTAGAAATT+CGG + Chr7:78235337-78235356 None:intergenic 25.0%
!! ATTCAGCCAACATTTTAAAT+TGG - Chr7:78234585-78234604 MsG0780040482.01.T01:intron 25.0%
!!! ACAGTTTTAAGGATTTATTC+AGG - Chr7:78235001-78235020 MsG0780040482.01.T01:intron 25.0%
ACTGTTTATCACATCTTTGT+AGG - Chr7:78235214-78235233 MsG0780040482.01.T01:CDS 30.0%
CACATTAATCCAAATGTTCT+TGG + Chr7:78235249-78235268 None:intergenic 30.0%
GGAGCTTCTCATTTATATTA+TGG - Chr7:78234617-78234636 MsG0780040482.01.T01:intron 30.0%
TAATCCAAATGTTCTTGGTA+AGG + Chr7:78235244-78235263 None:intergenic 30.0%
TCTATCTGGCAATAATAAGT+TGG - Chr7:78235048-78235067 MsG0780040482.01.T01:CDS 30.0%
TTAATTGTTTCGTGTAACAG+TGG + Chr7:78235101-78235120 None:intergenic 30.0%
! AAACATAGTGTAGGTTGAAT+CGG + Chr7:78235291-78235310 None:intergenic 30.0%
AGATAGAGTTTGACTGCTAT+AGG + Chr7:78235035-78235054 None:intergenic 35.0%
CAGACAAGAATGATGTCAAT+GGG + Chr7:78234077-78234096 None:intergenic 35.0%
CAGTAGTAACACTATCATGT+TGG + Chr7:78234554-78234573 None:intergenic 35.0%
GAACATTTGGATTAATGTGC+AGG - Chr7:78235250-78235269 MsG0780040482.01.T01:CDS 35.0%
GAGAAACCTTCAAATCATGA+TGG - Chr7:78233985-78234004 MsG0780040482.01.T01:CDS 35.0%
GATAGAGTTTGACTGCTATA+GGG + Chr7:78235034-78235053 None:intergenic 35.0%
TATAGCAGTCAAACTCTATC+TGG - Chr7:78235034-78235053 MsG0780040482.01.T01:CDS 35.0%
TATTGATCCCATAACCAGTT+TGG + Chr7:78234831-78234850 None:intergenic 35.0%
TTCACCTTACCAAGAACATT+TGG - Chr7:78235237-78235256 MsG0780040482.01.T01:CDS 35.0%
TTGTAAGGTTGAGTTAGTGT+AGG - Chr7:78235154-78235173 MsG0780040482.01.T01:CDS 35.0%
! AACATAGTGTAGGTTGAATC+GGG + Chr7:78235290-78235309 None:intergenic 35.0%
! ACACTATCATGTTGGTCTAA+TGG + Chr7:78234546-78234565 None:intergenic 35.0%
! ACCTTACAAACCAGGTTTTA+TGG + Chr7:78235143-78235162 None:intergenic 35.0%
! GTGAAAGTATGAATGAAGCT+AGG - Chr7:78234950-78234969 MsG0780040482.01.T01:intron 35.0%
!! CCTTACAAACCAGGTTTTAT+GGG + Chr7:78235142-78235161 None:intergenic 35.0%
!! CTTACAAACCAGGTTTTATG+GGG + Chr7:78235141-78235160 None:intergenic 35.0%
AAGTCCATCCAAACTGGTTA+TGG - Chr7:78234820-78234839 MsG0780040482.01.T01:intron 40.0%
ACATTGCAATGGGAATGAGT+GGG - Chr7:78234025-78234044 MsG0780040482.01.T01:CDS 40.0%
AGTCCATCCAAACTGGTTAT+GGG - Chr7:78234821-78234840 MsG0780040482.01.T01:intron 40.0%
CATTGGATCAACAGTGTTGT+AGG + Chr7:78234115-78234134 None:intergenic 40.0%
CTAACTCAACCTTACAAACC+AGG + Chr7:78235151-78235170 None:intergenic 40.0%
CTACAACACTGTTGATCCAA+TGG - Chr7:78234113-78234132 MsG0780040482.01.T01:intron 40.0%
GCAGACAAGAATGATGTCAA+TGG + Chr7:78234078-78234097 None:intergenic 40.0%
TTGCTGCCATCATGATTTGA+AGG + Chr7:78233994-78234013 None:intergenic 40.0%
! ATAGTCAATGAGTTGGATCG+TGG - Chr7:78233952-78233971 MsG0780040482.01.T01:CDS 40.0%
! CCCATAAAACCTGGTTTGTA+AGG - Chr7:78235139-78235158 MsG0780040482.01.T01:CDS 40.0%
! CTGGCAATAATAAGTTGGCA+GGG - Chr7:78235053-78235072 MsG0780040482.01.T01:CDS 40.0%
! TAGTCAATGAGTTGGATCGT+GGG - Chr7:78233953-78233972 MsG0780040482.01.T01:CDS 40.0%
! TCTGGCAATAATAAGTTGGC+AGG - Chr7:78235052-78235071 MsG0780040482.01.T01:CDS 40.0%
!! GATCGACTTGACATTGCAAT+GGG - Chr7:78234015-78234034 MsG0780040482.01.T01:CDS 40.0%
!! TGATCGACTTGACATTGCAA+TGG - Chr7:78234014-78234033 MsG0780040482.01.T01:CDS 40.0%
ACTCATCAGCAAATGTCGTG+TGG + Chr7:78234873-78234892 None:intergenic 45.0%
CAACCTCAGATGTACGAGAA+AGG + Chr7:78234185-78234204 None:intergenic 45.0%
CATTGCAATGGGAATGAGTG+GGG - Chr7:78234026-78234045 MsG0780040482.01.T01:CDS 45.0%
CCTACACTATGTTTCAGCCA+CGG - Chr7:78235297-78235316 MsG0780040482.01.T01:CDS 45.0%
CTACACTATGTTTCAGCCAC+GGG - Chr7:78235298-78235317 MsG0780040482.01.T01:CDS 45.0%
GACATTGCAATGGGAATGAG+TGG - Chr7:78234024-78234043 MsG0780040482.01.T01:CDS 45.0%
GATCCCATAACCAGTTTGGA+TGG + Chr7:78234827-78234846 None:intergenic 45.0%
GTGTTGTAGGCTGTCAAAAG+TGG + Chr7:78234102-78234121 None:intergenic 45.0%
TCAGTCGACAGGACTGTATA+TGG - Chr7:78235181-78235200 MsG0780040482.01.T01:CDS 45.0%
! CTTTCTCGTACATCTGAGGT+TGG - Chr7:78234183-78234202 MsG0780040482.01.T01:intron 45.0%
ATGAGAGATGTCCAGAGAGC+AGG + Chr7:78234144-78234163 None:intergenic 50.0%
CAATGGTAGATCCTGCTCTC+TGG - Chr7:78234130-78234149 MsG0780040482.01.T01:intron 50.0%
CAGAGAGCAGGATCTACCAT+TGG + Chr7:78234132-78234151 None:intergenic 50.0%
CCGTGGCTGAAACATAGTGT+AGG + Chr7:78235300-78235319 None:intergenic 50.0%
CTCAGATGTACGAGAAAGGC+CGG + Chr7:78234181-78234200 None:intergenic 50.0%
CTGGAGAAGTCCATCCAAAC+TGG - Chr7:78234814-78234833 MsG0780040482.01.T01:intron 50.0%
GTGTAGGAGAATCAGTCGAC+AGG - Chr7:78235170-78235189 MsG0780040482.01.T01:CDS 50.0%
TCGTACAGCCCCATAAAACC+TGG - Chr7:78235130-78235149 MsG0780040482.01.T01:CDS 50.0%
! CAATGAGTTGGATCGTGGGA+AGG - Chr7:78233957-78233976 MsG0780040482.01.T01:CDS 50.0%
CGGCCTTTCTCGTACATCTG+AGG - Chr7:78234179-78234198 MsG0780040482.01.T01:intron 55.0%
CTCATTCCCATGCTTCCTGC+CGG - Chr7:78234159-78234178 MsG0780040482.01.T01:intron 55.0%
GTTTCAGCCACGGGAGTGAT+TGG - Chr7:78235307-78235326 MsG0780040482.01.T01:CDS 55.0%
AGAAAGGCCGGCAGGAAGCA+TGG + Chr7:78234169-78234188 None:intergenic 60.0%
CGGATGTCCAATCACTCCCG+TGG + Chr7:78235317-78235336 None:intergenic 60.0%
GATGTACGAGAAAGGCCGGC+AGG + Chr7:78234177-78234196 None:intergenic 60.0%
! GAAAGGCCGGCAGGAAGCAT+GGG + Chr7:78234168-78234187 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 78233931 78235374 78233931 ID=MsG0780040482.01;Name=MsG0780040482.01
Chr7 mRNA 78233931 78235374 78233931 ID=MsG0780040482.01.T01;Parent=MsG0780040482.01;Name=MsG0780040482.01.T01;_AED=0.49;_eAED=0.72;_QI=0|0|0|1|0|0|2|0|164
Chr7 exon 78235019 78235374 78235019 ID=MsG0780040482.01.T01:exon:16119;Parent=MsG0780040482.01.T01
Chr7 exon 78233931 78234069 78233931 ID=MsG0780040482.01.T01:exon:16118;Parent=MsG0780040482.01.T01
Chr7 CDS 78235019 78235374 78235019 ID=MsG0780040482.01.T01:cds;Parent=MsG0780040482.01.T01
Chr7 CDS 78233931 78234069 78233931 ID=MsG0780040482.01.T01:cds;Parent=MsG0780040482.01.T01
Gene Sequence

>MsG0780040482.01.T01

ATGACGAGAATGAAAACAAAAATAGTCAATGAGTTGGATCGTGGGAAGGCAGTAGAGAAACCTTCAAATCATGATGGCAGCAATGATCGACTTGACATTGCAATGGGAATGAGTGGGGCGTTGTCTCTTCAAAGAAATGTTTACCCATTGACATCATTCTTGTCTGCACCACTTTTGACAGCCTACAACACTGTTGATCCAATGGTAGATCCTGCTCTCTGGACATCTCTCATTCCCATGCTTCCTGCCGGCCTTTCTCGTACATCTGAGGTTGGTATAATGACTAAAGTATTTTACTTTATTCACAAGGAAGAAAGATCACATGAAATCTCATTTAACAATGCTAAAAAGGCTCAGTTCACCTTACCAAGAACATTTGGATTAATGTGCAGGTTACAAAGACAGAGCCCGATTCAACCTACACTATGTTTCAGCCACGGGAGTGATTGGACATCCGAATTTCTAATTGAAGAAAGCCAAGAGCCTGAAAGCTGA

Protein sequence

>MsG0780040482.01.T01

MTRMKTKIVNELDRGKAVEKPSNHDGSNDRLDIAMGMSGALSLQRNVYPLTSFLSAPLLTAYNTVDPMVDPALWTSLIPMLPAGLSRTSEVGIMTKVFYFIHKEERSHEISFNNAKKAQFTLPRTFGLMCRLQRQSPIQPTLCFSHGSDWTSEFLIEESQEPES*