AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040523.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040523.01.T01 MTR_7g092070 99.571 233 1 0 1 233 323 555 1.15e-165 477
MsG0780040523.01.T01 MTR_3g029590 71.186 236 58 6 1 231 323 553 9.04e-106 323
MsG0780040523.01.T01 MTR_1g050438 57.203 236 86 5 2 234 288 511 6.90e-74 237
MsG0780040523.01.T01 MTR_0728s0020 57.203 236 86 5 2 234 304 527 1.33e-73 237
MsG0780040523.01.T01 MTR_5g071070 43.396 212 89 7 2 190 208 411 2.15e-42 154
MsG0780040523.01.T01 MTR_5g071070 43.396 212 89 7 2 190 290 493 3.38e-42 154
MsG0780040523.01.T01 MTR_5g082830 44.856 243 96 8 2 225 277 500 4.50e-42 153
MsG0780040523.01.T01 MTR_3g435680 48.851 174 54 5 2 173 211 351 6.95e-40 143
MsG0780040523.01.T01 MTR_3g435590 60.000 100 35 1 1 100 297 391 2.86e-33 127
MsG0780040523.01.T01 MTR_1g075890 42.929 198 79 6 3 195 290 458 3.17e-33 128
MsG0780040523.01.T01 MTR_3g464260 43.005 193 84 5 2 194 241 407 1.05e-32 126
MsG0780040523.01.T01 MTR_3g464280 41.753 194 88 6 2 195 214 382 2.09e-28 114
MsG0780040523.01.T01 MTR_3g464270 36.598 194 99 4 2 195 70 239 1.53e-26 107
MsG0780040523.01.T01 MTR_3g070880 38.286 175 63 5 2 168 223 360 5.43e-25 104
MsG0780040523.01.T01 MTR_2g090605 72.549 51 14 0 2 52 137 187 1.65e-20 90.9
MsG0780040523.01.T01 MTR_6g090380 83.721 43 7 0 2 44 163 205 2.37e-20 90.1
MsG0780040523.01.T01 MTR_8g102100 49.020 102 38 2 2 95 141 236 3.32e-20 89.7
MsG0780040523.01.T01 MTR_3g103960 37.662 154 73 4 2 145 114 254 7.62e-19 84.3
MsG0780040523.01.T01 MTR_4g078660 72.093 43 12 0 2 44 120 162 1.85e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040523.01.T01 AT2G41900 72.689 238 42 7 1 233 320 539 1.76e-104 320
MsG0780040523.01.T01 AT5G12850 59.833 239 80 6 1 234 316 543 1.37e-86 273
MsG0780040523.01.T01 AT5G58620 48.768 203 73 7 2 194 269 450 4.37e-43 155
MsG0780040523.01.T01 AT2G40140 41.919 198 70 5 2 194 274 431 1.08e-32 126
MsG0780040523.01.T01 AT2G40140 41.919 198 70 5 2 194 274 431 1.08e-32 126
MsG0780040523.01.T01 AT3G55980 51.351 148 44 8 2 139 271 400 1.47e-31 123
MsG0780040523.01.T01 AT3G55980 49.342 152 41 8 2 139 338 467 2.94e-31 122
MsG0780040523.01.T01 AT4G29190 48.148 108 53 2 2 106 139 246 1.32e-20 90.5
MsG0780040523.01.T01 AT1G03790 66.102 59 17 1 2 57 143 201 9.83e-20 88.6
MsG0780040523.01.T01 AT2G19810 73.333 45 12 0 2 46 138 182 4.98e-19 86.3
MsG0780040523.01.T01 AT2G25900 73.333 45 12 0 2 46 150 194 1.22e-18 84.7
MsG0780040523.01.T01 AT2G25900 73.333 45 12 0 2 46 150 194 1.22e-18 84.7
MsG0780040523.01.T01 AT5G44260 68.889 45 14 0 2 46 121 165 3.75e-17 80.9
MsG0780040523.01.T01 AT5G07500 72.093 43 12 0 2 44 112 154 3.85e-16 76.6

Find 71 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTGCAGGCGGCTTCTTA+TGG 0.247506 7:+78771062 MsG0780040523.01.T01:CDS
AAAACAGTTGAAGTCAGATT+TGG 0.296997 7:-78770937 None:intergenic
TACAATGGCCGAAGCTCTTC+AGG 0.319804 7:-78770457 None:intergenic
ATCCCAGCCTTGCATCTTCC+TGG 0.322304 7:+78770673 MsG0780040523.01.T01:CDS
GCAGCCATGAGCATGTTGCC+TGG 0.353518 7:+78770544 MsG0780040523.01.T01:CDS
ACAGGCATGCCAACTCATTA+AGG 0.364336 7:-78770798 None:intergenic
CGGCCATTGTATGTTTCAAC+TGG 0.366588 7:+78770469 MsG0780040523.01.T01:CDS
AAGAGATCATCAAGATTTGA+TGG 0.375352 7:-78770880 None:intergenic
TCAAGATTTGATGGATTCAA+TGG 0.378043 7:-78770871 None:intergenic
CTTGAGCCCATTGGAGAGAT+AGG 0.383762 7:-78771125 None:intergenic
TTGTTGCTGTTGATCATAAT+CGG 0.386100 7:-78770773 None:intergenic
CTAGACTGAAGATTACTTCC+AGG 0.391284 7:-78770691 None:intergenic
AGATGCAAGGCTGGGATGTT+TGG 0.402195 7:-78770667 None:intergenic
TGTTGATCATCATTTATTGC+AGG 0.414822 7:+78771049 MsG0780040523.01.T01:CDS
TTTCACACAACTCTGTTGCT+TGG 0.421951 7:+78770638 MsG0780040523.01.T01:CDS
TCTTATGGTGTTCAGCCATC+AGG 0.430030 7:+78771077 MsG0780040523.01.T01:CDS
CGTGGAGACATTCTTCCTGA+TGG 0.449642 7:-78771092 None:intergenic
ACTCCACCCATGTCACCTTC+TGG 0.450662 7:+78770607 MsG0780040523.01.T01:CDS
GGCAAAGAAGCAAACTCTCC+TGG 0.456158 7:-78770428 None:intergenic
CTTCCAGGAAGATGCAAGGC+TGG 0.462334 7:-78770676 None:intergenic
GACATCGAAGAAGGTGAGCC+AGG 0.468116 7:-78770562 None:intergenic
AATTAATATGACGTGGAGAC+AGG 0.471650 7:-78770816 None:intergenic
AGGACGGTACAAATTGTTCC+AGG 0.478546 7:+78770410 MsG0780040523.01.T01:CDS
GGTGACATGGGTGGAGTGAA+TGG 0.492460 7:-78770601 None:intergenic
GTCAAGCACGTCTTCTGCCA+TGG 0.494988 7:+78770513 MsG0780040523.01.T01:CDS
CAATGCTAGAGATATTCATA+TGG 0.504807 7:+78770732 MsG0780040523.01.T01:CDS
CCCACACTCCTGAAGAGCTT+CGG 0.505759 7:+78770449 MsG0780040523.01.T01:CDS
TGTACCGTCCTTGCAGAGAC+GGG 0.511148 7:-78770398 None:intergenic
GTGGAACCTATCTCTCCAAT+GGG 0.516161 7:+78771119 MsG0780040523.01.T01:CDS
GAGATAGGTTCCACGTTTCG+TGG 0.525659 7:-78771110 None:intergenic
CTATCTCTCCAATGGGCTCA+AGG 0.530752 7:+78771126 MsG0780040523.01.T01:CDS
ATTGACATCCTTGAGCCCAT+TGG 0.533162 7:-78771134 None:intergenic
AAGACAGCTGATTTGTGAGT+CGG 0.538872 7:-78770964 None:intergenic
GACATGTGTGAATATGCTCA+TGG 0.543598 7:+78770337 None:intergenic
CGTGGAACCTATCTCTCCAA+TGG 0.547113 7:+78771118 MsG0780040523.01.T01:CDS
GGGCTCAAGGATGTCAATGC+TGG 0.547672 7:+78771139 MsG0780040523.01.T01:CDS
GCCGAAGCTCTTCAGGAGTG+TGG 0.548910 7:-78770450 None:intergenic
AGACGGGTTCTATATTGTGC+AGG 0.553860 7:-78770382 None:intergenic
ATTCAATGGCTTCATTCGAC+CGG 0.556611 7:-78770857 None:intergenic
CCCATGTCACCTTCTGGCAA+TGG 0.558647 7:+78770613 MsG0780040523.01.T01:CDS
TTCCAGGAAGATGCAAGGCT+GGG 0.563396 7:-78770675 None:intergenic
TTGTACCGTCCTTGCAGAGA+CGG 0.564971 7:-78770399 None:intergenic
GACATGACAGACATCGAAGA+AGG 0.569249 7:-78770571 None:intergenic
CATGGCTGCAGCAAAATCCA+TGG 0.577281 7:-78770530 None:intergenic
GTATGTTTCAACTGGTTCTG+CGG 0.583036 7:+78770477 MsG0780040523.01.T01:CDS
TGAGCCAGGCAACATGCTCA+TGG 0.584222 7:-78770548 None:intergenic
GACGTGCTTGACCGAGGTGA+GGG 0.585321 7:-78770502 None:intergenic
CCGAAGCTCTTCAGGAGTGT+GGG 0.587103 7:-78770449 None:intergenic
GCAACAACTCCTTAATGAGT+TGG 0.589263 7:+78770789 MsG0780040523.01.T01:CDS
GTTCTGCGGTTCCCTCACCT+CGG 0.594015 7:+78770491 MsG0780040523.01.T01:CDS
AGACGTGCTTGACCGAGGTG+AGG 0.601319 7:-78770503 None:intergenic
AAGAATGTCTCCACGAAACG+TGG 0.605224 7:+78771100 MsG0780040523.01.T01:CDS
TCTAACAGTCTTAGCCGCTC+CGG 0.606773 7:+78770838 MsG0780040523.01.T01:CDS
GCCATTGCCAGAAGGTGACA+TGG 0.611555 7:-78770614 None:intergenic
CTGTTAGAATTAATATGACG+TGG 0.612704 7:-78770823 None:intergenic
ATATAGAACCCGTCTCTGCA+AGG 0.614108 7:+78770390 MsG0780040523.01.T01:CDS
TGATCATCATTTATTGCAGG+CGG 0.614691 7:+78771052 MsG0780040523.01.T01:CDS
ATATCGAGGAGAAGAACTCT+CGG 0.618533 7:-78770908 None:intergenic
ACATGTGTGAATATGCTCAT+GGG 0.619210 7:+78770338 None:intergenic
AAGGCTGGGATGTTTGGCTG+AGG 0.620227 7:-78770661 None:intergenic
ATGGGTGGAGTGAATGGTGA+AGG 0.622440 7:-78770595 None:intergenic
TTGCCAGAAGGTGACATGGG+TGG 0.627061 7:-78770610 None:intergenic
AGAACCCGTCTCTGCAAGGA+CGG 0.642420 7:+78770394 MsG0780040523.01.T01:CDS
CATGTGTGAATATGCTCATG+GGG 0.652271 7:+78770339 None:intergenic
GCAGAAGACGTGCTTGACCG+AGG 0.653400 7:-78770508 None:intergenic
TGTGAAATGCCATTGCCAGA+AGG 0.659354 7:-78770622 None:intergenic
ATTACTTCCAGGAAGATGCA+AGG 0.661108 7:-78770680 None:intergenic
ATGGCTTCATTCGACCGGAG+CGG 0.667016 7:-78770852 None:intergenic
GAACCAGTTGAAACATACAA+TGG 0.685763 7:-78770472 None:intergenic
AGATTTGGATCAGCATATCG+AGG 0.694701 7:-78770922 None:intergenic
CCATTGCCAGAAGGTGACAT+GGG 0.719317 7:-78770613 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAAAAATTGTATTCAC+TGG - Chr7:78771024-78771043 None:intergenic 25.0%
AAAACAGTTGAAGTCAGATT+TGG - Chr7:78770940-78770959 None:intergenic 30.0%
AAGAGATCATCAAGATTTGA+TGG - Chr7:78770883-78770902 None:intergenic 30.0%
CAATGCTAGAGATATTCATA+TGG + Chr7:78770732-78770751 MsG0780040523.01.T01:CDS 30.0%
CTGTTAGAATTAATATGACG+TGG - Chr7:78770826-78770845 None:intergenic 30.0%
TGTTGATCATCATTTATTGC+AGG + Chr7:78771049-78771068 MsG0780040523.01.T01:CDS 30.0%
! TTGTTGCTGTTGATCATAAT+CGG - Chr7:78770776-78770795 None:intergenic 30.0%
!! TCAAGATTTGATGGATTCAA+TGG - Chr7:78770874-78770893 None:intergenic 30.0%
AATTAATATGACGTGGAGAC+AGG - Chr7:78770819-78770838 None:intergenic 35.0%
GAACCAGTTGAAACATACAA+TGG - Chr7:78770475-78770494 None:intergenic 35.0%
TGATCATCATTTATTGCAGG+CGG + Chr7:78771052-78771071 MsG0780040523.01.T01:CDS 35.0%
AGATTTGGATCAGCATATCG+AGG - Chr7:78770925-78770944 None:intergenic 40.0%
ATATCGAGGAGAAGAACTCT+CGG - Chr7:78770911-78770930 None:intergenic 40.0%
ATTACTTCCAGGAAGATGCA+AGG - Chr7:78770683-78770702 None:intergenic 40.0%
ATTCAATGGCTTCATTCGAC+CGG - Chr7:78770860-78770879 None:intergenic 40.0%
CTAGACTGAAGATTACTTCC+AGG - Chr7:78770694-78770713 None:intergenic 40.0%
GCAACAACTCCTTAATGAGT+TGG + Chr7:78770789-78770808 MsG0780040523.01.T01:CDS 40.0%
TTTCACACAACTCTGTTGCT+TGG + Chr7:78770638-78770657 MsG0780040523.01.T01:CDS 40.0%
!! AAGACAGCTGATTTGTGAGT+CGG - Chr7:78770967-78770986 None:intergenic 40.0%
!! GTATGTTTCAACTGGTTCTG+CGG + Chr7:78770477-78770496 MsG0780040523.01.T01:CDS 40.0%
AAGAATGTCTCCACGAAACG+TGG + Chr7:78771100-78771119 MsG0780040523.01.T01:CDS 45.0%
ACAGGCATGCCAACTCATTA+AGG - Chr7:78770801-78770820 None:intergenic 45.0%
AGGACGGTACAAATTGTTCC+AGG + Chr7:78770410-78770429 MsG0780040523.01.T01:CDS 45.0%
ATATAGAACCCGTCTCTGCA+AGG + Chr7:78770390-78770409 MsG0780040523.01.T01:CDS 45.0%
ATTGACATCCTTGAGCCCAT+TGG - Chr7:78771137-78771156 None:intergenic 45.0%
CGGCCATTGTATGTTTCAAC+TGG + Chr7:78770469-78770488 MsG0780040523.01.T01:CDS 45.0%
GACATGACAGACATCGAAGA+AGG - Chr7:78770574-78770593 None:intergenic 45.0%
GTGGAACCTATCTCTCCAAT+GGG + Chr7:78771119-78771138 MsG0780040523.01.T01:CDS 45.0%
TCTTATGGTGTTCAGCCATC+AGG + Chr7:78771077-78771096 MsG0780040523.01.T01:CDS 45.0%
TGTGAAATGCCATTGCCAGA+AGG - Chr7:78770625-78770644 None:intergenic 45.0%
TTATTGCAGGCGGCTTCTTA+TGG + Chr7:78771062-78771081 MsG0780040523.01.T01:CDS 45.0%
! AGACGGGTTCTATATTGTGC+AGG - Chr7:78770385-78770404 None:intergenic 45.0%
AGATGCAAGGCTGGGATGTT+TGG - Chr7:78770670-78770689 None:intergenic 50.0%
ATGGGTGGAGTGAATGGTGA+AGG - Chr7:78770598-78770617 None:intergenic 50.0%
CATGGCTGCAGCAAAATCCA+TGG - Chr7:78770533-78770552 None:intergenic 50.0%
CCATTGCCAGAAGGTGACAT+GGG - Chr7:78770616-78770635 None:intergenic 50.0%
CGTGGAACCTATCTCTCCAA+TGG + Chr7:78771118-78771137 MsG0780040523.01.T01:CDS 50.0%
CGTGGAGACATTCTTCCTGA+TGG - Chr7:78771095-78771114 None:intergenic 50.0%
CTTGAGCCCATTGGAGAGAT+AGG - Chr7:78771128-78771147 None:intergenic 50.0%
GAGATAGGTTCCACGTTTCG+TGG - Chr7:78771113-78771132 None:intergenic 50.0%
GGCAAAGAAGCAAACTCTCC+TGG - Chr7:78770431-78770450 None:intergenic 50.0%
TACAATGGCCGAAGCTCTTC+AGG - Chr7:78770460-78770479 None:intergenic 50.0%
TCTAACAGTCTTAGCCGCTC+CGG + Chr7:78770838-78770857 MsG0780040523.01.T01:CDS 50.0%
TTCCAGGAAGATGCAAGGCT+GGG - Chr7:78770678-78770697 None:intergenic 50.0%
TTGTACCGTCCTTGCAGAGA+CGG - Chr7:78770402-78770421 None:intergenic 50.0%
! CTATCTCTCCAATGGGCTCA+AGG + Chr7:78771126-78771145 MsG0780040523.01.T01:CDS 50.0%
AAGGCTGGGATGTTTGGCTG+AGG - Chr7:78770664-78770683 None:intergenic 55.0%
ACTCCACCCATGTCACCTTC+TGG + Chr7:78770607-78770626 MsG0780040523.01.T01:CDS 55.0%
AGAACCCGTCTCTGCAAGGA+CGG + Chr7:78770394-78770413 MsG0780040523.01.T01:CDS 55.0%
ATCCCAGCCTTGCATCTTCC+TGG + Chr7:78770673-78770692 MsG0780040523.01.T01:CDS 55.0%
ATGGCTTCATTCGACCGGAG+CGG - Chr7:78770855-78770874 None:intergenic 55.0%
CCCACACTCCTGAAGAGCTT+CGG + Chr7:78770449-78770468 MsG0780040523.01.T01:CDS 55.0%
CCCATGTCACCTTCTGGCAA+TGG + Chr7:78770613-78770632 MsG0780040523.01.T01:CDS 55.0%
CTTCCAGGAAGATGCAAGGC+TGG - Chr7:78770679-78770698 None:intergenic 55.0%
GACATCGAAGAAGGTGAGCC+AGG - Chr7:78770565-78770584 None:intergenic 55.0%
GCCATTGCCAGAAGGTGACA+TGG - Chr7:78770617-78770636 None:intergenic 55.0%
GGGCTCAAGGATGTCAATGC+TGG + Chr7:78771139-78771158 MsG0780040523.01.T01:CDS 55.0%
GGTGACATGGGTGGAGTGAA+TGG - Chr7:78770604-78770623 None:intergenic 55.0%
GTCAAGCACGTCTTCTGCCA+TGG + Chr7:78770513-78770532 MsG0780040523.01.T01:CDS 55.0%
TGAGCCAGGCAACATGCTCA+TGG - Chr7:78770551-78770570 None:intergenic 55.0%
TGTACCGTCCTTGCAGAGAC+GGG - Chr7:78770401-78770420 None:intergenic 55.0%
TTGCCAGAAGGTGACATGGG+TGG - Chr7:78770613-78770632 None:intergenic 55.0%
! CCGAAGCTCTTCAGGAGTGT+GGG - Chr7:78770452-78770471 None:intergenic 55.0%
!!! CTCATGGGGTTTTCGAGTGC+TGG + Chr7:78770353-78770372 MsG0780040523.01.T01:CDS 55.0%
AGACGTGCTTGACCGAGGTG+AGG - Chr7:78770506-78770525 None:intergenic 60.0%
GACGTGCTTGACCGAGGTGA+GGG - Chr7:78770505-78770524 None:intergenic 60.0%
GCAGAAGACGTGCTTGACCG+AGG - Chr7:78770511-78770530 None:intergenic 60.0%
GCAGCCATGAGCATGTTGCC+TGG + Chr7:78770544-78770563 MsG0780040523.01.T01:CDS 60.0%
GCCGAAGCTCTTCAGGAGTG+TGG - Chr7:78770453-78770472 None:intergenic 60.0%
GTTCTGCGGTTCCCTCACCT+CGG + Chr7:78770491-78770510 MsG0780040523.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 78770340 78771197 78770340 ID=MsG0780040523.01;Name=MsG0780040523.01
Chr7 mRNA 78770340 78771197 78770340 ID=MsG0780040523.01.T01;Parent=MsG0780040523.01;Name=MsG0780040523.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|285
Chr7 exon 78770340 78771197 78770340 ID=MsG0780040523.01.T01:exon:16121;Parent=MsG0780040523.01.T01
Chr7 CDS 78770340 78771197 78770340 ID=MsG0780040523.01.T01:cds;Parent=MsG0780040523.01.T01
Gene Sequence

>MsG0780040523.01.T01

ATGTGTGAATATGCTCATGGGGTTTTCGAGTGCTGGCTGCATCCTGCACAATATAGAACCCGTCTCTGCAAGGACGGTACAAATTGTTCCAGGAGAGTTTGCTTCTTTGCCCACACTCCTGAAGAGCTTCGGCCATTGTATGTTTCAACTGGTTCTGCGGTTCCCTCACCTCGGTCAAGCACGTCTTCTGCCATGGATTTTGCTGCAGCCATGAGCATGTTGCCTGGCTCACCTTCTTCGATGTCTGTCATGTCTCCTTCACCATTCACTCCACCCATGTCACCTTCTGGCAATGGCATTTCACACAACTCTGTTGCTTGGCCTCAGCCAAACATCCCAGCCTTGCATCTTCCTGGAAGTAATCTTCAGTCTAGTCGTTTGAGATCTTCTCTCAATGCTAGAGATATTCATATGGATGATTTTGAGTTGTTATCCGATTATGATCAACAGCAACAACTCCTTAATGAGTTGGCATGCCTGTCTCCACGTCATATTAATTCTAACAGTCTTAGCCGCTCCGGTCGAATGAAGCCATTGAATCCATCAAATCTTGATGATCTCTTTTCTGCCGAGAGTTCTTCTCCTCGATATGCTGATCCAAATCTGACTTCAACTGTTTTTTCTCCGACTCACAAATCAGCTGTCTTTAATCAGTTTCAGCAGCAGCAGAACATGTTGTCACCAGTGAATACAATTTTTCTTCTAAAAATGTTGATCATCATTTATTGCAGGCGGCTTCTTATGGTGTTCAGCCATCAGGAAGAATGTCTCCACGAAACGTGGAACCTATCTCTCCAATGGGCTCAAGGATGTCAATGCTGGCACAACGCGACAAGCAGCAACAATTTCGTAGCCTAA

Protein sequence

>MsG0780040523.01.T01

MCEYAHGVFECWLHPAQYRTRLCKDGTNCSRRVCFFAHTPEELRPLYVSTGSAVPSPRSSTSSAMDFAAAMSMLPGSPSSMSVMSPSPFTPPMSPSGNGISHNSVAWPQPNIPALHLPGSNLQSSRLRSSLNARDIHMDDFELLSDYDQQQQLLNELACLSPRHINSNSLSRSGRMKPLNPSNLDDLFSAESSSPRYADPNLTSTVFSPTHKSAVFNQFQQQQNMLSPVNTIFLLKMLIIIYCRRLLMVFSHQEECLHETWNLSLQWAQGCQCWHNATSSNNFVA*