Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004844.01.T01 | XP_003591500.1 | 93.827 | 162 | 8 | 1 | 1 | 160 | 1 | 162 | 1.95E-94 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004844.01.T01 | G7IEM0 | 93.827 | 162 | 8 | 1 | 1 | 160 | 1 | 162 | 9.30e-95 | 280 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001795.01 | MsG0180004844.01 | -0.833759 | 4.434495e-56 | 5.156263e-53 |
| MsG0180003128.01 | MsG0180004844.01 | -0.828275 | 9.844135e-55 | 9.738071e-52 |
| MsG0180003129.01 | MsG0180004844.01 | -0.863115 | 3.134247e-64 | 9.395989e-61 |
| MsG0180004844.01 | MsG0280006671.01 | 0.806221 | 9.138308e-50 | 4.932879e-47 |
| MsG0180004844.01 | MsG0280007477.01 | 0.819739 | 9.915022e-53 | 7.694417e-50 |
| MsG0180004844.01 | MsG0380012661.01 | 0.810314 | 1.225641e-50 | 7.363806e-48 |
| MsG0180004844.01 | MsG0380016315.01 | 0.808969 | 2.384537e-50 | 1.382574e-47 |
| MsG0180004844.01 | MsG0480018497.01 | 0.822948 | 1.802367e-53 | 1.530573e-50 |
| MsG0180004844.01 | MsG0480021249.01 | -0.813933 | 1.991723e-51 | 1.318189e-48 |
| MsG0180004844.01 | MsG0480021251.01 | -0.830321 | 3.136758e-55 | 3.293518e-52 |
| MsG0180004844.01 | MsG0580025985.01 | 0.821879 | 3.193125e-53 | 2.630432e-50 |
| MsG0180004844.01 | MsG0580029166.01 | -0.824760 | 6.780147e-54 | 6.062053e-51 |
| MsG0180004844.01 | MsG0580029256.01 | -0.807092 | 5.984591e-50 | 3.303399e-47 |
| MsG0180004844.01 | MsG0780040523.01 | 0.813461 | 2.530167e-51 | 1.653449e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004844.01.T01 | MTR_1g088250 | 93.827 | 162 | 8 | 1 | 1 | 160 | 1 | 162 | 2.36e-98 | 280 |
| MsG0180004844.01.T01 | MTR_1g088260 | 48.819 | 127 | 64 | 1 | 31 | 157 | 3 | 128 | 2.27e-44 | 142 |
| MsG0180004844.01.T01 | MTR_7g108775 | 47.222 | 144 | 73 | 2 | 6 | 147 | 11 | 153 | 1.75e-43 | 141 |
| MsG0180004844.01.T01 | MTR_7g108775 | 47.222 | 144 | 73 | 2 | 6 | 147 | 11 | 153 | 3.00e-43 | 142 |
| MsG0180004844.01.T01 | MTR_6g086680 | 36.364 | 143 | 80 | 4 | 5 | 136 | 7 | 149 | 8.25e-23 | 89.0 |
| MsG0180004844.01.T01 | MTR_8g085580 | 32.520 | 123 | 77 | 3 | 17 | 136 | 23 | 142 | 2.66e-15 | 69.7 |
| MsG0180004844.01.T01 | MTR_7g080280 | 28.226 | 124 | 84 | 2 | 23 | 143 | 40 | 161 | 5.18e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004844.01.T01 | AT1G02816 | 61.151 | 139 | 52 | 1 | 22 | 160 | 30 | 166 | 2.87e-60 | 184 |
| MsG0180004844.01.T01 | AT4G02370 | 53.503 | 157 | 68 | 2 | 8 | 160 | 12 | 167 | 9.47e-56 | 173 |
| MsG0180004844.01.T01 | AT4G02360 | 54.545 | 143 | 64 | 1 | 2 | 144 | 7 | 148 | 5.37e-54 | 168 |
| MsG0180004844.01.T01 | AT1G02813 | 45.139 | 144 | 76 | 2 | 3 | 144 | 2 | 144 | 3.85e-41 | 135 |
| MsG0180004844.01.T01 | AT5G19590 | 33.871 | 124 | 75 | 2 | 24 | 146 | 31 | 148 | 2.43e-17 | 74.7 |
| MsG0180004844.01.T01 | AT5G19590 | 33.871 | 124 | 75 | 2 | 24 | 146 | 31 | 148 | 2.43e-17 | 74.7 |
| MsG0180004844.01.T01 | AT5G16380 | 28.221 | 163 | 98 | 6 | 5 | 154 | 12 | 168 | 2.57e-14 | 67.8 |
| MsG0180004844.01.T01 | AT5G19860 | 34.234 | 111 | 67 | 3 | 23 | 130 | 35 | 142 | 1.50e-13 | 65.5 |
| MsG0180004844.01.T01 | AT3G07470 | 29.630 | 135 | 84 | 4 | 18 | 149 | 25 | 151 | 1.65e-13 | 64.7 |
| MsG0180004844.01.T01 | AT3G07470 | 28.571 | 147 | 92 | 5 | 8 | 149 | 13 | 151 | 1.68e-13 | 64.7 |
| MsG0180004844.01.T01 | AT3G07470 | 28.571 | 147 | 92 | 5 | 8 | 149 | 13 | 151 | 2.41e-13 | 64.3 |
| MsG0180004844.01.T01 | AT1G55265 | 34.711 | 121 | 71 | 4 | 25 | 141 | 55 | 171 | 1.61e-11 | 59.7 |
| MsG0180004844.01.T01 | AT5G01610 | 29.592 | 98 | 68 | 1 | 26 | 123 | 56 | 152 | 8.71e-11 | 57.8 |
| MsG0180004844.01.T01 | AT5G01610 | 29.592 | 98 | 68 | 1 | 26 | 123 | 56 | 152 | 8.71e-11 | 57.8 |
Find 49 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGTAGGCAGTTGGGTTTC+CGG | 0.215272 | 1:-83475952 | None:intergenic |
| GCGTCAAAGTGATGTTCTTC+TGG | 0.264271 | 1:+83476177 | MsG0180004844.01.T01:CDS |
| AAAATAGACTTACCGATCTA+AGG | 0.267447 | 1:+83476147 | MsG0180004844.01.T01:CDS |
| TGATTTGTCTAATTCGTAAC+TGG | 0.313925 | 1:-83476024 | None:intergenic |
| TGAATATTCTTGAAGTTGTT+AGG | 0.334073 | 1:+83476201 | MsG0180004844.01.T01:CDS |
| TCCTCAGTGTGGTTGTGGTT+TGG | 0.365789 | 1:+83476295 | MsG0180004844.01.T01:CDS |
| ACTCCTATCGGGAGAATTCC+CGG | 0.413850 | 1:-83475999 | None:intergenic |
| CGGTTAGCGCCGTCGCCGTC+AGG | 0.414867 | 1:-83475926 | None:intergenic |
| GAATCTCCTCAGTGTGGTTG+TGG | 0.417985 | 1:+83476290 | MsG0180004844.01.T01:CDS |
| TCGTGTAGTTTCTCGTTACA+AGG | 0.427526 | 1:+83476077 | MsG0180004844.01.T01:CDS |
| CGCAAGGCCTCGTAGGCAGT+TGG | 0.436838 | 1:-83475960 | None:intergenic |
| AGGAGGAGGAGGAGAACGAC+GGG | 0.447449 | 1:-83475906 | None:intergenic |
| GGCAGTTGGGTTTCCGGTGA+CGG | 0.448621 | 1:-83475946 | None:intergenic |
| CGTCAAAGTGATGTTCTTCT+GGG | 0.456544 | 1:+83476178 | MsG0180004844.01.T01:CDS |
| CTCTGTTGGTGTTGCTTCTG+CGG | 0.459033 | 1:+83476244 | MsG0180004844.01.T01:CDS |
| TTTAAGCAGAAGAAAGAGAA+GGG | 0.461680 | 1:-83476358 | None:intergenic |
| TCGTAACTGGTTACTCCTAT+CGG | 0.463432 | 1:-83476011 | None:intergenic |
| GCGCCGTCGCCGTCAGGAGG+AGG | 0.477696 | 1:-83475920 | None:intergenic |
| AAATAGACTTACCGATCTAA+GGG | 0.481492 | 1:+83476148 | MsG0180004844.01.T01:CDS |
| GCAAGGCCTCGTAGGCAGTT+GGG | 0.489260 | 1:-83475959 | None:intergenic |
| CGTAACTGGTTACTCCTATC+GGG | 0.502559 | 1:-83476010 | None:intergenic |
| AACGATCACCGGTAAAATAA+CGG | 0.509312 | 1:+83476124 | MsG0180004844.01.T01:CDS |
| CCGTCGCCGTCAGGAGGAGG+AGG | 0.512636 | 1:-83475917 | None:intergenic |
| GATGACCTTGATTTCTCTGT+TGG | 0.513791 | 1:+83476230 | MsG0180004844.01.T01:CDS |
| AATTCCCGGTGGGAAATTGT+AGG | 0.515251 | 1:-83475985 | None:intergenic |
| CAACACCAACAGAGAAATCA+AGG | 0.526235 | 1:-83476235 | None:intergenic |
| GCGACGGCGCTAACCGTCAC+CGG | 0.534133 | 1:+83475933 | MsG0180004844.01.T01:CDS |
| GACGCTTATACCCCTTAGAT+CGG | 0.549482 | 1:-83476159 | None:intergenic |
| CGTACCTACAATTTCCCACC+GGG | 0.555651 | 1:+83475981 | MsG0180004844.01.T01:CDS |
| CCTCCTCCTCCTGACGGCGA+CGG | 0.559285 | 1:+83475917 | MsG0180004844.01.T01:CDS |
| TTCTTTGAATCTCCTCAGTG+TGG | 0.575291 | 1:+83476284 | MsG0180004844.01.T01:CDS |
| TTAGCGCCGTCGCCGTCAGG+AGG | 0.577589 | 1:-83475923 | None:intergenic |
| GCGTACCTACAATTTCCCAC+CGG | 0.578143 | 1:+83475980 | MsG0180004844.01.T01:CDS |
| CAGGAGGAGGAGGAGAACGA+CGG | 0.578931 | 1:-83475907 | None:intergenic |
| CCACCGGGAATTCTCCCGAT+AGG | 0.583587 | 1:+83475996 | MsG0180004844.01.T01:CDS |
| AGTTACAAATCAACGATCAC+CGG | 0.584572 | 1:+83476113 | MsG0180004844.01.T01:CDS |
| TGGGAAATTGTAGGTACGCA+AGG | 0.589209 | 1:-83475976 | None:intergenic |
| CGTTCTCCTCCTCCTCCTGA+CGG | 0.595497 | 1:+83475911 | MsG0180004844.01.T01:CDS |
| TACGAATTAGACAAATCAAG+TGG | 0.598160 | 1:+83476029 | MsG0180004844.01.T01:CDS |
| CGGAAACCCAACTGCCTACG+AGG | 0.623801 | 1:+83475953 | MsG0180004844.01.T01:CDS |
| GTAACGAGAAACTACACGAA+CGG | 0.631098 | 1:-83476073 | None:intergenic |
| GTAGGTACGCAAGGCCTCGT+AGG | 0.643114 | 1:-83475967 | None:intergenic |
| GATTCAAAGAAATTATCCAA+AGG | 0.666602 | 1:-83476272 | None:intergenic |
| CTATCGGGAGAATTCCCGGT+GGG | 0.669781 | 1:-83475995 | None:intergenic |
| AATAGACTTACCGATCTAAG+GGG | 0.686558 | 1:+83476149 | MsG0180004844.01.T01:CDS |
| GGAGGAGGAGGAGAACGACG+GGG | 0.724686 | 1:-83475905 | None:intergenic |
| TCCAAACCACAACCACACTG+AGG | 0.753988 | 1:-83476296 | None:intergenic |
| CCTATCGGGAGAATTCCCGG+TGG | 0.777816 | 1:-83475996 | None:intergenic |
| ACGAACGGTTTAGATCAGCG+CGG | 0.852399 | 1:-83476058 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATTCAAAGAAATTATCCAA+AGG | - | Chr1:83476275-83476294 | None:intergenic | 25.0% |
| ! | TGAATATTCTTGAAGTTGTT+AGG | + | Chr1:83476201-83476220 | MsG0180004844.01.T01:CDS | 25.0% |
| !!! | TCTATTTTCCGTTATTTTAC+CGG | - | Chr1:83476135-83476154 | None:intergenic | 25.0% |
| AAAATAGACTTACCGATCTA+AGG | + | Chr1:83476147-83476166 | MsG0180004844.01.T01:CDS | 30.0% | |
| AAATAGACTTACCGATCTAA+GGG | + | Chr1:83476148-83476167 | MsG0180004844.01.T01:CDS | 30.0% | |
| TACGAATTAGACAAATCAAG+TGG | + | Chr1:83476029-83476048 | MsG0180004844.01.T01:CDS | 30.0% | |
| ! | TGATTTGTCTAATTCGTAAC+TGG | - | Chr1:83476027-83476046 | None:intergenic | 30.0% |
| AACGATCACCGGTAAAATAA+CGG | + | Chr1:83476124-83476143 | MsG0180004844.01.T01:CDS | 35.0% | |
| AATAGACTTACCGATCTAAG+GGG | + | Chr1:83476149-83476168 | MsG0180004844.01.T01:CDS | 35.0% | |
| AGTTACAAATCAACGATCAC+CGG | + | Chr1:83476113-83476132 | MsG0180004844.01.T01:CDS | 35.0% | |
| CAACACCAACAGAGAAATCA+AGG | - | Chr1:83476238-83476257 | None:intergenic | 40.0% | |
| CGTCAAAGTGATGTTCTTCT+GGG | + | Chr1:83476178-83476197 | MsG0180004844.01.T01:CDS | 40.0% | |
| GATGACCTTGATTTCTCTGT+TGG | + | Chr1:83476230-83476249 | MsG0180004844.01.T01:CDS | 40.0% | |
| GTAACGAGAAACTACACGAA+CGG | - | Chr1:83476076-83476095 | None:intergenic | 40.0% | |
| TCGTAACTGGTTACTCCTAT+CGG | - | Chr1:83476014-83476033 | None:intergenic | 40.0% | |
| TCGTGTAGTTTCTCGTTACA+AGG | + | Chr1:83476077-83476096 | MsG0180004844.01.T01:CDS | 40.0% | |
| TTCTTTGAATCTCCTCAGTG+TGG | + | Chr1:83476284-83476303 | MsG0180004844.01.T01:CDS | 40.0% | |
| AATTCCCGGTGGGAAATTGT+AGG | - | Chr1:83475988-83476007 | None:intergenic | 45.0% | |
| CGTAACTGGTTACTCCTATC+GGG | - | Chr1:83476013-83476032 | None:intergenic | 45.0% | |
| GACGCTTATACCCCTTAGAT+CGG | - | Chr1:83476162-83476181 | None:intergenic | 45.0% | |
| GCGTCAAAGTGATGTTCTTC+TGG | + | Chr1:83476177-83476196 | MsG0180004844.01.T01:CDS | 45.0% | |
| TGGGAAATTGTAGGTACGCA+AGG | - | Chr1:83475979-83475998 | None:intergenic | 45.0% | |
| ACGAACGGTTTAGATCAGCG+CGG | - | Chr1:83476061-83476080 | None:intergenic | 50.0% | |
| ACTCCTATCGGGAGAATTCC+CGG | - | Chr1:83476002-83476021 | None:intergenic | 50.0% | |
| CGTACCTACAATTTCCCACC+GGG | + | Chr1:83475981-83476000 | MsG0180004844.01.T01:CDS | 50.0% | |
| GAATCTCCTCAGTGTGGTTG+TGG | + | Chr1:83476290-83476309 | MsG0180004844.01.T01:CDS | 50.0% | |
| GCGTACCTACAATTTCCCAC+CGG | + | Chr1:83475980-83475999 | MsG0180004844.01.T01:CDS | 50.0% | |
| TCCAAACCACAACCACACTG+AGG | - | Chr1:83476299-83476318 | None:intergenic | 50.0% | |
| TCCTCAGTGTGGTTGTGGTT+TGG | + | Chr1:83476295-83476314 | MsG0180004844.01.T01:CDS | 50.0% | |
| ! | TGCTTCTGCGGCTTTTCCTT+TGG | + | Chr1:83476256-83476275 | MsG0180004844.01.T01:CDS | 50.0% |
| !! | CTCTGTTGGTGTTGCTTCTG+CGG | + | Chr1:83476244-83476263 | MsG0180004844.01.T01:CDS | 50.0% |
| CTATCGGGAGAATTCCCGGT+GGG | - | Chr1:83475998-83476017 | None:intergenic | 55.0% | |
| CTCGTAGGCAGTTGGGTTTC+CGG | - | Chr1:83475955-83475974 | None:intergenic | 55.0% | |
| AGGAGGAGGAGGAGAACGAC+GGG | - | Chr1:83475909-83475928 | None:intergenic | 60.0% | |
| CAGGAGGAGGAGGAGAACGA+CGG | - | Chr1:83475910-83475929 | None:intergenic | 60.0% | |
| CCACCGGGAATTCTCCCGAT+AGG | + | Chr1:83475996-83476015 | MsG0180004844.01.T01:CDS | 60.0% | |
| CCTATCGGGAGAATTCCCGG+TGG | - | Chr1:83475999-83476018 | None:intergenic | 60.0% | |
| CGGAAACCCAACTGCCTACG+AGG | + | Chr1:83475953-83475972 | MsG0180004844.01.T01:CDS | 60.0% | |
| CGTTCTCCTCCTCCTCCTGA+CGG | + | Chr1:83475911-83475930 | MsG0180004844.01.T01:CDS | 60.0% | |
| GCAAGGCCTCGTAGGCAGTT+GGG | - | Chr1:83475962-83475981 | None:intergenic | 60.0% | |
| GGCAGTTGGGTTTCCGGTGA+CGG | - | Chr1:83475949-83475968 | None:intergenic | 60.0% | |
| GTAGGTACGCAAGGCCTCGT+AGG | - | Chr1:83475970-83475989 | None:intergenic | 60.0% | |
| CGCAAGGCCTCGTAGGCAGT+TGG | - | Chr1:83475963-83475982 | None:intergenic | 65.0% | |
| GGAGGAGGAGGAGAACGACG+GGG | - | Chr1:83475908-83475927 | None:intergenic | 65.0% | |
| CCTCCTCCTCCTGACGGCGA+CGG | + | Chr1:83475917-83475936 | MsG0180004844.01.T01:CDS | 70.0% | |
| GCGACGGCGCTAACCGTCAC+CGG | + | Chr1:83475933-83475952 | MsG0180004844.01.T01:CDS | 70.0% | |
| TTAGCGCCGTCGCCGTCAGG+AGG | - | Chr1:83475926-83475945 | None:intergenic | 70.0% | |
| CCGTCGCCGTCAGGAGGAGG+AGG | - | Chr1:83475920-83475939 | None:intergenic | 75.0% | |
| CGGTTAGCGCCGTCGCCGTC+AGG | - | Chr1:83475929-83475948 | None:intergenic | 75.0% | |
| ! | GCGCCGTCGCCGTCAGGAGG+AGG | - | Chr1:83475923-83475942 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 83475897 | 83476379 | 83475897 | ID=MsG0180004844.01;Name=MsG0180004844.01 |
| Chr1 | mRNA | 83475897 | 83476379 | 83475897 | ID=MsG0180004844.01.T01;Parent=MsG0180004844.01;Name=MsG0180004844.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|160 |
| Chr1 | exon | 83475897 | 83476379 | 83475897 | ID=MsG0180004844.01.T01:exon:27619;Parent=MsG0180004844.01.T01 |
| Chr1 | CDS | 83475897 | 83476379 | 83475897 | ID=MsG0180004844.01.T01:cds;Parent=MsG0180004844.01.T01 |
| Gene Sequence |
| Protein sequence |
