AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040827.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040827.01.T01 MTR_7g099210 77.841 176 10 1 1 147 1 176 2.15e-89 276
MsG0780040827.01.T01 MTR_1g069355 82.308 130 23 0 18 147 79 208 1.04e-71 228
MsG0780040827.01.T01 MTR_1g069355 82.308 130 23 0 18 147 79 208 1.27e-71 229
MsG0780040827.01.T01 MTR_4g119620 33.000 100 61 3 44 140 85 181 2.48e-12 63.2
MsG0780040827.01.T01 MTR_3g106080 40.789 76 44 1 41 116 549 623 3.06e-12 63.2
MsG0780040827.01.T01 MTR_1g072140 34.000 100 60 3 44 140 76 172 5.97e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040827.01.T01 AT1G04140 73.571 140 37 0 8 147 74 213 5.18e-67 217
MsG0780040827.01.T01 AT1G04140 73.571 140 37 0 8 147 74 213 6.88e-67 216
MsG0780040827.01.T01 AT5G43930 78.740 127 27 0 18 144 87 213 1.58e-64 209
MsG0780040827.01.T01 AT5G43930 78.740 127 27 0 18 144 87 213 1.58e-64 209
MsG0780040827.01.T01 AT5G43930 78.740 127 27 0 18 144 87 213 1.58e-64 209
MsG0780040827.01.T01 AT5G43930 66.225 151 27 1 18 144 87 237 2.91e-59 195
MsG0780040827.01.T01 AT3G49660 35.714 98 61 2 44 140 78 174 3.36e-15 71.2

Find 48 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATCAAGAAGTTCGATTA+TGG 0.243621 7:-83009360 MsG0780040827.01.T01:CDS
CGTTGCTTAAAGTGTTTAAT+TGG 0.247471 7:-83009610 MsG0780040827.01.T01:CDS
AATTAAAGAATATTCTAATA+TGG 0.306006 7:-83010667 MsG0780040827.01.T01:five_prime_UTR
CGTGTTCGTGTTCGTGTTCA+AGG 0.309647 7:+83010592 None:intergenic
AAGATAATCGACTGTGAAAC+TGG 0.350714 7:-83009634 MsG0780040827.01.T01:CDS
AACGGCAATGATTTCCCCTT+TGG 0.368185 7:+83009138 None:intergenic
GGATCAAGAAGTTCGATTAT+GGG 0.386752 7:-83009359 MsG0780040827.01.T01:CDS
GAAATCATTGCCGTTGCATC+AGG 0.388142 7:-83009130 MsG0780040827.01.T01:CDS
CCACCCAAGGCGTCCTCATA+TGG 0.404101 7:+83009587 None:intergenic
TTACCTGCCGTTATAATTAC+CGG 0.420052 7:+83010369 None:intergenic
CTCCCACTAGCAAGTATTTG+TGG 0.430838 7:+83009385 None:intergenic
CCCTTTGGCATGAAAAGCAA+TGG 0.446896 7:+83009153 None:intergenic
AATACTTGCTAGTGGGAGCT+TGG 0.475100 7:-83009380 MsG0780040827.01.T01:CDS
TGAAAAGCAATGGATGCAAT+TGG 0.476696 7:+83009163 None:intergenic
GAAAATGATCCTTAGGGTTG+AGG 0.480101 7:-83009866 MsG0780040827.01.T01:intron
TACCTTGTGGCCTGATGCAA+CGG 0.492040 7:+83009120 None:intergenic
ACCTTCACCGTCGTCTCGCT+AGG 0.494262 7:-83010623 MsG0780040827.01.T01:five_prime_UTR
GAATCAGAGAGAGTAATAGT+AGG 0.497721 7:+83010496 None:intergenic
AGTGTTTAATTGGCCATATG+AGG 0.500364 7:-83009600 MsG0780040827.01.T01:CDS
TGGCCATATGAGGACGCCTT+GGG 0.511608 7:-83009590 MsG0780040827.01.T01:CDS
GATTTCAAGCTTACCACCCA+AGG 0.513791 7:+83009574 None:intergenic
TTGGCCATATGAGGACGCCT+TGG 0.541707 7:-83009591 MsG0780040827.01.T01:CDS
CACGAACACGAACACGAACA+CGG 0.542530 7:-83010585 MsG0780040827.01.T01:five_prime_UTR
ACCTAGCGAGACGACGGTGA+AGG 0.558637 7:+83010622 None:intergenic
GCAGCGGCGTTTAGTCCAGA+TGG 0.579480 7:-83009778 MsG0780040827.01.T01:CDS
GTTCTTTAGTACCTGCGCCA+AGG 0.582270 7:-83009810 MsG0780040827.01.T01:CDS
AGAAAGTAAATATACAGAAG+TGG 0.582698 7:+83009305 None:intergenic
AGGTACTAAAGAACAATACT+TGG 0.589341 7:+83009819 None:intergenic
CATCGGAATCGTGATCGCAC+CGG 0.590292 7:-83010388 MsG0780040827.01.T01:CDS
TGCCGTTGCATCAGGCCACA+AGG 0.593080 7:-83009122 MsG0780040827.01.T01:CDS
GCAAGTATTTGTGGATGCAA+TGG 0.594535 7:+83009394 None:intergenic
GATCGCACCGGTAATTATAA+CGG 0.595029 7:-83010376 MsG0780040827.01.T01:CDS
CACATATGACGAGAATTCAT+CGG 0.599243 7:-83010405 MsG0780040827.01.T01:exon
GCCGCTGCAATAGTAGACCT+TGG 0.604711 7:+83009793 None:intergenic
GCACCGGTAATTATAACGGC+AGG 0.608085 7:-83010372 MsG0780040827.01.T01:intron
GTATTTGTGGATGCAATGGA+TGG 0.608429 7:+83009398 None:intergenic
CCATATGAGGACGCCTTGGG+TGG 0.612145 7:-83009587 MsG0780040827.01.T01:intron
ACACGAACACGAACACGGAG+AGG 0.616284 7:-83010580 MsG0780040827.01.T01:five_prime_UTR
CATCCACAAATACTTGCTAG+TGG 0.619566 7:-83009388 MsG0780040827.01.T01:CDS
ATCCACAAATACTTGCTAGT+GGG 0.630308 7:-83009387 MsG0780040827.01.T01:CDS
AACGAGAAGAAACAGAAGAG+AGG 0.651443 7:+83010470 None:intergenic
GCAATAGTAGACCTTGGCGC+AGG 0.654426 7:+83009799 None:intergenic
ATTATGGGATGCTAACACAT+CGG 0.657460 7:-83009344 MsG0780040827.01.T01:CDS
GCCAAGGTCTACTATTGCAG+CGG 0.665562 7:-83009794 MsG0780040827.01.T01:CDS
GTATAATTAACACAAGTCGT+AGG 0.672001 7:+83010644 None:intergenic
GCAGAGACCTAGCGAGACGA+CGG 0.673343 7:+83010616 None:intergenic
AGTCGATTATCTTTACTGTG+TGG 0.679994 7:+83009644 None:intergenic
GGGTTGAGGCTGATTCACTG+CGG 0.741601 7:-83009852 MsG0780040827.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAAGAATATTCTAATA+TGG - Chr7:83009105-83009124 MsG0780040827.01.T01:CDS 10.0%
!!! GTATTTTTATAATTGAATTA+TGG - Chr7:83009506-83009525 MsG0780040827.01.T01:intron 10.0%
!! AACAAAAAAATTAGTAATAG+TGG + Chr7:83010526-83010545 None:intergenic 15.0%
!! TTCAATTTAAACTAATAATG+AGG + Chr7:83010063-83010082 None:intergenic 15.0%
!! TTTAGCACATAAATTAAAAT+TGG + Chr7:83010337-83010356 None:intergenic 15.0%
!! AATAGATGCATAATAAATTC+AGG + Chr7:83010234-83010253 None:intergenic 20.0%
!! ACAAAAAAAACTAATCACAA+AGG + Chr7:83010089-83010108 None:intergenic 20.0%
!! CAAAAAAAACTAATCACAAA+GGG + Chr7:83010088-83010107 None:intergenic 20.0%
!! TTTCTGTTTGATGAATTTAT+TGG - Chr7:83010567-83010586 MsG0780040827.01.T01:five_prime_UTR 20.0%
! AAAATTAGTAATAGTGGCAA+AGG + Chr7:83010520-83010539 None:intergenic 25.0%
! AGAAAGTAAATATACAGAAG+TGG + Chr7:83010470-83010489 None:intergenic 25.0%
! ATTCAACAAATTCAACACAA+CGG + Chr7:83009728-83009747 None:intergenic 25.0%
! GAGAAATGATTAATCAGAAA+CGG + Chr7:83009436-83009455 None:intergenic 25.0%
! TACAATCCATATAAAAACTG+AGG + Chr7:83009675-83009694 None:intergenic 25.0%
! TGTTTAAATACCATGACAAT+AGG + Chr7:83009831-83009850 None:intergenic 25.0%
!! ATCTTTGAAAATGATCCTTA+GGG - Chr7:83009900-83009919 MsG0780040827.01.T01:intron 25.0%
!!! GTTGTTTTTCTTGTATTGAT+TGG - Chr7:83010543-83010562 MsG0780040827.01.T01:five_prime_UTR 25.0%
!!! TTTTTGTTTGTTATGTGTAG+TGG - Chr7:83010102-83010121 MsG0780040827.01.T01:intron 25.0%
AATCATTTCTCTGTTTCCAT+CGG - Chr7:83009445-83009464 MsG0780040827.01.T01:intron 30.0%
AGAAAAAAACTACTACCTTG+TGG + Chr7:83010668-83010687 None:intergenic 30.0%
AGGTACTAAAGAACAATACT+TGG + Chr7:83009956-83009975 None:intergenic 30.0%
ATTCCTTCTGTAAACAACAA+AGG + Chr7:83010297-83010316 None:intergenic 30.0%
GTATAATTAACACAAGTCGT+AGG + Chr7:83009131-83009150 None:intergenic 30.0%
TCAGTCTATAAACGATATCT+AGG - Chr7:83009854-83009873 MsG0780040827.01.T01:CDS 30.0%
TGCATTTAGATCGAATCATT+TGG - Chr7:83009475-83009494 MsG0780040827.01.T01:intron 30.0%
TTTATGTGCTAAATGACTAC+AGG - Chr7:83010344-83010363 MsG0780040827.01.T01:intron 30.0%
TTTCAAAGATGTTAGTCAAG+AGG + Chr7:83009890-83009909 None:intergenic 30.0%
! AGTTTGCCTCAGTTTTTATA+TGG - Chr7:83009666-83009685 MsG0780040827.01.T01:intron 30.0%
! CATCTTTGAAAATGATCCTT+AGG - Chr7:83009899-83009918 MsG0780040827.01.T01:intron 30.0%
! CGTTGCTTAAAGTGTTTAAT+TGG - Chr7:83010162-83010181 MsG0780040827.01.T01:intron 30.0%
!! ATGTCTTTAATTTGCTTGCA+CGG - Chr7:83009755-83009774 MsG0780040827.01.T01:intron 30.0%
AAGATAATCGACTGTGAAAC+TGG - Chr7:83010138-83010157 MsG0780040827.01.T01:intron 35.0%
AGTCGATTATCTTTACTGTG+TGG + Chr7:83010131-83010150 None:intergenic 35.0%
AGTGTTTAATTGGCCATATG+AGG - Chr7:83010172-83010191 MsG0780040827.01.T01:intron 35.0%
ATTATGGGATGCTAACACAT+CGG - Chr7:83010428-83010447 MsG0780040827.01.T01:five_prime_UTR 35.0%
CACATATGACGAGAATTCAT+CGG - Chr7:83009367-83009386 MsG0780040827.01.T01:CDS 35.0%
CATAATAAATTCAGGCAGAC+AGG + Chr7:83010226-83010245 None:intergenic 35.0%
CTAATTTGAGCTGATCCTAA+TGG + Chr7:83009594-83009613 None:intergenic 35.0%
GAATCAGAGAGAGTAATAGT+AGG + Chr7:83009279-83009298 None:intergenic 35.0%
GGATCAAGAAGTTCGATTAT+GGG - Chr7:83010413-83010432 MsG0780040827.01.T01:exon 35.0%
TGAAAAGCAATGGATGCAAT+TGG + Chr7:83010612-83010631 None:intergenic 35.0%
TGGATCAAGAAGTTCGATTA+TGG - Chr7:83010412-83010431 MsG0780040827.01.T01:exon 35.0%
TTACCTGCCGTTATAATTAC+CGG + Chr7:83009406-83009425 None:intergenic 35.0%
! ATCCACAAATACTTGCTAGT+GGG - Chr7:83010385-83010404 MsG0780040827.01.T01:CDS 35.0%
!! CTTGCTACATGTTTTGTTCT+CGG + Chr7:83009793-83009812 None:intergenic 35.0%
!! TTGCCTTTGTTGTTTACAGA+AGG - Chr7:83010291-83010310 MsG0780040827.01.T01:intron 35.0%
AACGAGAAGAAACAGAAGAG+AGG + Chr7:83009305-83009324 None:intergenic 40.0%
ACTAATTGCAACTTACTGCG+TGG + Chr7:83010034-83010053 None:intergenic 40.0%
CTAAATGCAACTGAAACCGA+TGG + Chr7:83009464-83009483 None:intergenic 40.0%
GAAAATGATCCTTAGGGTTG+AGG - Chr7:83009906-83009925 MsG0780040827.01.T01:intron 40.0%
GCAAGTATTTGTGGATGCAA+TGG + Chr7:83010381-83010400 None:intergenic 40.0%
GTATTTGTGGATGCAATGGA+TGG + Chr7:83010377-83010396 None:intergenic 40.0%
GTGCTAAATGACTACAGGTT+AGG - Chr7:83010349-83010368 MsG0780040827.01.T01:intron 40.0%
GTGCTCGTTTATTGACCATT+AGG - Chr7:83009576-83009595 MsG0780040827.01.T01:intron 40.0%
! CATCCACAAATACTTGCTAG+TGG - Chr7:83010384-83010403 MsG0780040827.01.T01:CDS 40.0%
! GATCGCACCGGTAATTATAA+CGG - Chr7:83009396-83009415 MsG0780040827.01.T01:CDS 40.0%
! GATGCAAGAACTTTTCCATC+TGG + Chr7:83010012-83010031 None:intergenic 40.0%
! TCTTTTTCTCTCTCTGTCTC+TGG + Chr7:83009239-83009258 None:intergenic 40.0%
!! CATTGCTTTTCATGCCAAAG+GGG - Chr7:83010620-83010639 MsG0780040827.01.T01:five_prime_UTR 40.0%
!! CCATTGCTTTTCATGCCAAA+GGG - Chr7:83010619-83010638 MsG0780040827.01.T01:five_prime_UTR 40.0%
!! TCCATTGCTTTTCATGCCAA+AGG - Chr7:83010618-83010637 MsG0780040827.01.T01:five_prime_UTR 40.0%
AACGGCAATGATTTCCCCTT+TGG + Chr7:83010637-83010656 None:intergenic 45.0%
CCCTTTGGCATGAAAAGCAA+TGG + Chr7:83010622-83010641 None:intergenic 45.0%
CTCCCACTAGCAAGTATTTG+TGG + Chr7:83010390-83010409 None:intergenic 45.0%
GAAATCATTGCCGTTGCATC+AGG - Chr7:83010642-83010661 MsG0780040827.01.T01:five_prime_UTR 45.0%
GATTTCAAGCTTACCACCCA+AGG + Chr7:83010201-83010220 None:intergenic 45.0%
! AATACTTGCTAGTGGGAGCT+TGG - Chr7:83010392-83010411 MsG0780040827.01.T01:CDS 45.0%
! TTTTCTCTCTCTGTCTCTGG+TGG + Chr7:83009236-83009255 None:intergenic 45.0%
CAAAGCGTGGCCTATTGTCA+TGG - Chr7:83009818-83009837 MsG0780040827.01.T01:CDS 50.0%
CACGAACACGAACACGAACA+CGG - Chr7:83009187-83009206 MsG0780040827.01.T01:intron 50.0%
CGTGTTCGTGTTCGTGTTCA+AGG + Chr7:83009183-83009202 None:intergenic 50.0%
GCCAAGGTCTACTATTGCAG+CGG - Chr7:83009978-83009997 MsG0780040827.01.T01:intron 50.0%
GTAGCAAGAGATGCAAAGCG+TGG - Chr7:83009805-83009824 MsG0780040827.01.T01:CDS 50.0%
GTGAATCAGCCTCAACCCTA+AGG + Chr7:83009918-83009937 None:intergenic 50.0%
GTTCTTTAGTACCTGCGCCA+AGG - Chr7:83009962-83009981 MsG0780040827.01.T01:intron 50.0%
TACCTTGTGGCCTGATGCAA+CGG + Chr7:83010655-83010674 None:intergenic 50.0%
! GCACCGGTAATTATAACGGC+AGG - Chr7:83009400-83009419 MsG0780040827.01.T01:CDS 50.0%
ACACGAACACGAACACGGAG+AGG - Chr7:83009192-83009211 MsG0780040827.01.T01:intron 55.0%
CATCGGAATCGTGATCGCAC+CGG - Chr7:83009384-83009403 MsG0780040827.01.T01:CDS 55.0%
GCAATAGTAGACCTTGGCGC+AGG + Chr7:83009976-83009995 None:intergenic 55.0%
GCCGCTGCAATAGTAGACCT+TGG + Chr7:83009982-83010001 None:intergenic 55.0%
TGGCCATATGAGGACGCCTT+GGG - Chr7:83010182-83010201 MsG0780040827.01.T01:intron 55.0%
TTGGCCATATGAGGACGCCT+TGG - Chr7:83010181-83010200 MsG0780040827.01.T01:intron 55.0%
! GGGTTGAGGCTGATTCACTG+CGG - Chr7:83009920-83009939 MsG0780040827.01.T01:intron 55.0%
ACCTAGCGAGACGACGGTGA+AGG + Chr7:83009153-83009172 None:intergenic 60.0%
ACCTTCACCGTCGTCTCGCT+AGG - Chr7:83009149-83009168 MsG0780040827.01.T01:CDS 60.0%
CCATATGAGGACGCCTTGGG+TGG - Chr7:83010185-83010204 MsG0780040827.01.T01:intron 60.0%
GCAGAGACCTAGCGAGACGA+CGG + Chr7:83009159-83009178 None:intergenic 60.0%
GCAGCGGCGTTTAGTCCAGA+TGG - Chr7:83009994-83010013 MsG0780040827.01.T01:intron 60.0%
TGCCGTTGCATCAGGCCACA+AGG - Chr7:83010650-83010669 MsG0780040827.01.T01:five_prime_UTR 60.0%
! CCACCCAAGGCGTCCTCATA+TGG + Chr7:83010188-83010207 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83009105 83010689 83009105 ID=MsG0780040827.01;Name=MsG0780040827.01
Chr7 mRNA 83009105 83010689 83009105 ID=MsG0780040827.01.T01;Parent=MsG0780040827.01;Name=MsG0780040827.01.T01;_AED=0.45;_eAED=0.45;_QI=267|0.75|0.8|1|1|1|5|0|148
Chr7 exon 83009105 83009190 83009105 ID=MsG0780040827.01.T01:exon:38573;Parent=MsG0780040827.01.T01
Chr7 exon 83009320 83009428 83009320 ID=MsG0780040827.01.T01:exon:38572;Parent=MsG0780040827.01.T01
Chr7 exon 83009588 83009672 83009588 ID=MsG0780040827.01.T01:exon:38571;Parent=MsG0780040827.01.T01
Chr7 exon 83009757 83009873 83009757 ID=MsG0780040827.01.T01:exon:38570;Parent=MsG0780040827.01.T01
Chr7 exon 83010373 83010689 83010373 ID=MsG0780040827.01.T01:exon:38569;Parent=MsG0780040827.01.T01
Chr7 five_prime_UTR 83010423 83010689 83010423 ID=MsG0780040827.01.T01:five_prime_utr;Parent=MsG0780040827.01.T01
Chr7 CDS 83010373 83010422 83010373 ID=MsG0780040827.01.T01:cds;Parent=MsG0780040827.01.T01
Chr7 CDS 83009757 83009873 83009757 ID=MsG0780040827.01.T01:cds;Parent=MsG0780040827.01.T01
Chr7 CDS 83009588 83009672 83009588 ID=MsG0780040827.01.T01:cds;Parent=MsG0780040827.01.T01
Chr7 CDS 83009320 83009428 83009320 ID=MsG0780040827.01.T01:cds;Parent=MsG0780040827.01.T01
Chr7 CDS 83009105 83009190 83009105 ID=MsG0780040827.01.T01:cds;Parent=MsG0780040827.01.T01
Gene Sequence

>MsG0780040827.01.T01

ATGACGAGAATTCATCGGAATCGTGATCGCACCGGTAATTATAACGGCAGGGTTGAGGCTGATTCACTGCGGCATTTGTCAGCCAAGTATTGTTCTTTAGTACCTGCGCCAAGGTCTACTATTGCAGCGGCGTTTAGTCCAGATGGAAAAGTTCTTGCATCCACGCATGGTGACCACACAGTAAAGATAATCGACTGTGAAACTGGACGTTGCTTAAAGTGTTTAATTGGCCATATGAGGACGCCTTGGGTGGTTAGGTTCCATCCATTGCATCCACAAATACTTGCTAGTGGGAGCTTGGATCAAGAAGTTCGATTATGGGATGCTAACACATCGGAGTGCATAACATCTCACCACTTCTATCGACCAATTGCATCCATTGCTTTTCATGCCAAAGGGGAAATCATTGCCGTTGCATCAGGCCACAAGGTAGTAGTTTTTTTCTGA

Protein sequence

>MsG0780040827.01.T01

MTRIHRNRDRTGNYNGRVEADSLRHLSAKYCSLVPAPRSTIAAAFSPDGKVLASTHGDHTVKIIDCETGRCLKCLIGHMRTPWVVRFHPLHPQILASGSLDQEVRLWDANTSECITSHHFYRPIASIAFHAKGEIIAVASGHKVVVFF*