AlfalfaGEDB | MsG0480020463.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020463.01

KEGG: -
GO: -
PFAM: PF03168

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020463.01.T01	XP_003606085.1	93.662	142	7	1	1	142	80	219	1.05E-91	275

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020463.01.T01	Q9SRN0	54.225	142	62	1	1	142	71	209	6.62E-53	169

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020463.01.T01	G7JH86	93.662	142	7	1	1	142	80	219	5.01e-92	275

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049140.01	MsG0480020463.01	0.819147	1.352716e-52	1.032533e-49
MsG0180006000.01	MsG0480020463.01	0.807291	5.430177e-50	3.013031e-47
MsG0280010977.01	MsG0480020463.01	0.801068	1.071951e-48	5.071877e-46
MsG0480020463.01	MsG0480020495.01	0.820629	6.201144e-53	4.932170e-50
MsG0480020463.01	MsG0480020699.01	0.807035	6.152267e-50	3.391033e-47
MsG0480020463.01	MsG0480020847.01	0.836077	1.156084e-56	1.441257e-53
MsG0480020463.01	MsG0480021450.01	0.804135	2.498339e-49	1.278154e-46
MsG0480020463.01	MsG0480022784.01	0.807720	4.402994e-50	2.470485e-47
MsG0480020463.01	MsG0480022998.01	0.809009	2.338115e-50	1.357101e-47
MsG0480020463.01	MsG0480023262.01	0.807299	5.410418e-50	3.002682e-47
MsG0480020463.01	MsG0480023296.01	0.830313	3.149892e-55	3.306598e-52
MsG0480020463.01	MsG0480023672.01	0.831507	1.605330e-55	1.745650e-52
MsG0480020463.01	MsG0580024380.01	0.807027	6.176522e-50	3.403680e-47
MsG0480020463.01	MsG0580024489.01	0.816624	5.024458e-52	3.578373e-49
MsG0480020463.01	MsG0580024748.01	0.810358	1.199214e-50	7.213470e-48
MsG0480020463.01	MsG0580028756.01	0.832041	1.185497e-55	1.309643e-52
MsG0480020463.01	MsG0580029261.01	0.807469	4.979809e-50	2.776008e-47
MsG0480020463.01	MsG0680035878.01	0.811401	7.132379e-51	4.410816e-48
MsG0480020463.01	MsG0780035954.01	0.860453	2.043614e-63	5.589213e-60
MsG0480020463.01	MsG0780038332.01	0.800339	1.509692e-48	7.012067e-46
MsG0480020463.01	MsG0780040924.01	0.810307	1.229960e-50	7.388269e-48
MsG0480020463.01	MsG0780041063.01	0.818251	2.160684e-52	1.608953e-49
MsG0480020463.01	MsG0780041340.01	0.803882	2.820257e-49	1.433554e-46
MsG0480020463.01	MsG0880042773.01	0.844083	9.413889e-59	1.503011e-55
MsG0480020463.01	MsG0880043637.01	0.855574	5.758088e-62	1.335298e-58
MsG0480020463.01	MsG0880045430.01	0.811602	6.448792e-51	4.008967e-48
MsG0480020463.01	MsG0880047059.01	0.832430	9.498662e-56	1.061397e-52
MsG0380016703.01	MsG0480020463.01	0.805448	1.328628e-49	7.030872e-47
MsG0380017164.01	MsG0480020463.01	0.811772	5.923253e-51	3.698976e-48
MsG0480018397.01	MsG0480020463.01	0.814949	1.187126e-51	8.075765e-49
MsG0280006540.01	MsG0480020463.01	0.812092	5.044954e-51	3.177809e-48
MsG0280006563.01	MsG0480020463.01	0.802893	4.520141e-49	2.240014e-46
MsG0280009752.01	MsG0480020463.01	0.802602	5.189761e-49	2.552844e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020463.01.T01	MTR_4g051880	93.662	142	7	1	1	142	80	219	1.27e-95	275
MsG0480020463.01.T01	MTR_2g086790	62.238	143	51	2	1	142	81	221	1.46e-62	191
MsG0480020463.01.T01	MTR_7g106050	53.147	143	63	2	1	142	71	210	2.66e-50	159
MsG0480020463.01.T01	MTR_1g057420	50.694	144	64	2	1	142	71	209	4.94e-49	156
MsG0480020463.01.T01	MTR_7g106080	53.704	108	46	2	36	142	7	111	8.75e-36	119
MsG0480020463.01.T01	MTR_3g092790	39.823	113	68	0	1	113	76	188	1.04e-27	102
MsG0480020463.01.T01	MTR_8g024250	34.579	107	70	0	1	107	149	255	2.02e-15	71.2
MsG0480020463.01.T01	MTR_7g118270	28.972	107	76	0	1	107	97	203	5.86e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020463.01.T01	AT3G44220	54.861	144	55	2	1	142	71	206	1.76e-54	170
MsG0480020463.01.T01	AT3G11660	54.225	142	62	1	1	142	71	209	6.75e-54	169
MsG0480020463.01.T01	AT5G22200	46.479	142	70	2	1	142	75	210	1.25e-46	150
MsG0480020463.01.T01	AT2G35960	47.183	142	72	1	1	142	72	210	1.06e-45	148
MsG0480020463.01.T01	AT5G06330	47.183	142	68	2	1	142	73	207	6.56e-44	143
MsG0480020463.01.T01	AT3G52470	46.528	144	70	2	1	142	70	208	8.38e-44	143
MsG0480020463.01.T01	AT2G35970	41.844	141	79	1	1	141	73	210	1.52e-37	127
MsG0480020463.01.T01	AT4G09590	41.135	141	80	1	1	141	73	210	1.19e-36	125
MsG0480020463.01.T01	AT5G53730	38.053	113	70	0	1	113	80	192	1.12e-28	104
MsG0480020463.01.T01	AT4G01410	34.507	142	84	2	1	142	95	227	1.19e-24	94.7
MsG0480020463.01.T01	AT5G05657	40.860	93	49	3	1	91	44	132	1.23e-15	69.3

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGATGCCTATTCTGTTGTT+TGG	0.267291	4:-41995126	None:intergenic
TCAGAATAATGGAATGGTTT+TGG	0.280643	4:+41995317	MsG0480020463.01.T01:CDS
TTATGACCTTGGTAAGTATC+TGG	0.286360	4:-41995213	None:intergenic
TGAAGATTGCTTAGCGTAAA+AGG	0.309792	4:-41995288	None:intergenic
ATTTGGTCGCCGTTTATTTA+CGG	0.310515	4:+41995246	MsG0480020463.01.T01:CDS
TTGGTTAATATTAAGGTTAT+TGG	0.336031	4:+41995336	MsG0480020463.01.T01:CDS
TGAACTGTCCTGCTTATGTT+AGG	0.340610	4:+41995412	MsG0480020463.01.T01:CDS
CTTCAGCAGGATCAGAATAA+TGG	0.360367	4:+41995306	MsG0480020463.01.T01:CDS
CAACTCCGGCGGCATTATTC+CGG	0.373049	4:-41995452	None:intergenic
TTAAGTGGAGGGTTGGAAGT+TGG	0.386817	4:+41995364	MsG0480020463.01.T01:CDS
AGATGCCTATTCTGTTGTTT+GGG	0.392628	4:-41995125	None:intergenic
CTTAGCGTAAAAGGCGAAAC+CGG	0.402202	4:-41995279	None:intergenic
TATTTACGGCGTTGGCGTGC+CGG	0.414145	4:+41995260	MsG0480020463.01.T01:CDS
GTAAATAAACGGCGACCAAA+TGG	0.417716	4:-41995244	None:intergenic
ACGCCAACGCCGTAAATAAA+CGG	0.440550	4:-41995255	None:intergenic
AGGTTATTGGAAGGGTTAAG+TGG	0.445350	4:+41995349	MsG0480020463.01.T01:CDS
TATGTTAGGTTCTCCGCCTC+CGG	0.446962	4:+41995426	MsG0480020463.01.T01:CDS
TACGCTAAGCAATCTTCAGC+AGG	0.449107	4:+41995293	MsG0480020463.01.T01:CDS
TAAGTATCTGGAATTGAAGT+TGG	0.456345	4:-41995201	None:intergenic
GGTTCTCCGCCTCCGGTGAC+CGG	0.462032	4:+41995433	MsG0480020463.01.T01:CDS
TATTGGAAGGGTTAAGTGGA+GGG	0.476241	4:+41995353	MsG0480020463.01.T01:CDS
TGGTAAAATCTTTATGACCT+TGG	0.480324	4:-41995224	None:intergenic
TCGCCGTTTATTTACGGCGT+TGG	0.490468	4:+41995252	MsG0480020463.01.T01:CDS
ATTATTCCGGTCACCGGAGG+CGG	0.496180	4:-41995439	None:intergenic
CACAACCCAAACAACAGAAT+AGG	0.506911	4:+41995120	MsG0480020463.01.T01:CDS
GGAAGGGTTAAGTGGAGGGT+TGG	0.512565	4:+41995357	MsG0480020463.01.T01:CDS
CGCCGGAGTTGTTGCTCCGG+CGG	0.527356	4:+41995464	MsG0480020463.01.T01:CDS
AGGGTTGGAAGTTGGATCTC+CGG	0.534934	4:+41995372	MsG0480020463.01.T01:CDS
TGCCGCCGGAGTTGTTGCTC+CGG	0.544084	4:+41995461	MsG0480020463.01.T01:CDS
TAATATTAAGGTTATTGGAA+GGG	0.546343	4:+41995341	MsG0480020463.01.T01:CDS
TTAATATTAAGGTTATTGGA+AGG	0.548348	4:+41995340	MsG0480020463.01.T01:CDS
GGCGGCATTATTCCGGTCAC+CGG	0.556756	4:-41995445	None:intergenic
GCGGAGAACCTAACATAAGC+AGG	0.559676	4:-41995420	None:intergenic
GCAGGATCAGAATAATGGAA+TGG	0.594421	4:+41995311	MsG0480020463.01.T01:CDS
GGCATTATTCCGGTCACCGG+AGG	0.594682	4:-41995442	None:intergenic
AACCGCCGGAGCAACAACTC+CGG	0.597861	4:-41995466	None:intergenic
AAAAGCTGAAACTTAACCGC+CGG	0.610815	4:-41995480	None:intergenic
AAGTTGGTAGCGAGATCTGT+TGG	0.612269	4:-41995185	None:intergenic
TTATTGGAAGGGTTAAGTGG+AGG	0.618679	4:+41995352	MsG0480020463.01.T01:CDS
CGCCGGAGCAACAACTCCGG+CGG	0.631010	4:-41995463	None:intergenic
GGTGACCGGAATAATGCCGC+CGG	0.633130	4:+41995447	MsG0480020463.01.T01:CDS
TTGGAAGTTGGATCTCCGGA+AGG	0.666739	4:+41995376	MsG0480020463.01.T01:CDS
TCAATTCCAGATACTTACCA+AGG	0.693104	4:+41995207	MsG0480020463.01.T01:CDS
TCTTATAAGAAGCATAGACA+TGG	0.699623	4:-41995161	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATATTAAGGTTATTGGAA+GGG	+	Chr4:41995341-41995360	MsG0480020463.01.T01:CDS	20.0%
!!	TTAATATTAAGGTTATTGGA+AGG	+	Chr4:41995340-41995359	MsG0480020463.01.T01:CDS	20.0%
!!	TTGGTTAATATTAAGGTTAT+TGG	+	Chr4:41995336-41995355	MsG0480020463.01.T01:CDS	20.0%
!!!	AATGGTTTTGGTTAATATTA+AGG	+	Chr4:41995329-41995348	MsG0480020463.01.T01:CDS	20.0%
!!	GTCATAAAGATTTTACCATT+TGG	+	Chr4:41995229-41995248	MsG0480020463.01.T01:CDS	25.0%
	CACAAAAATATGATACCTTC+CGG	-	Chr4:41995394-41995413	None:intergenic	30.0%
	TGGTAAAATCTTTATGACCT+TGG	-	Chr4:41995227-41995246	None:intergenic	30.0%
!	TAAGTATCTGGAATTGAAGT+TGG	-	Chr4:41995204-41995223	None:intergenic	30.0%
!	TCTTATAAGAAGCATAGACA+TGG	-	Chr4:41995164-41995183	None:intergenic	30.0%
!!	TCAGAATAATGGAATGGTTT+TGG	+	Chr4:41995317-41995336	MsG0480020463.01.T01:CDS	30.0%
	ATTTGGTCGCCGTTTATTTA+CGG	+	Chr4:41995246-41995265	MsG0480020463.01.T01:CDS	35.0%
	TAGATGCCTATTCTGTTGTT+TGG	-	Chr4:41995129-41995148	None:intergenic	35.0%
	TCAATTCCAGATACTTACCA+AGG	+	Chr4:41995207-41995226	MsG0480020463.01.T01:CDS	35.0%
	TGAAGATTGCTTAGCGTAAA+AGG	-	Chr4:41995291-41995310	None:intergenic	35.0%
	TTATGACCTTGGTAAGTATC+TGG	-	Chr4:41995216-41995235	None:intergenic	35.0%
!	AGATGCCTATTCTGTTGTTT+GGG	-	Chr4:41995128-41995147	None:intergenic	35.0%
	AAAAGCTGAAACTTAACCGC+CGG	-	Chr4:41995483-41995502	None:intergenic	40.0%
	AGGTTATTGGAAGGGTTAAG+TGG	+	Chr4:41995349-41995368	MsG0480020463.01.T01:CDS	40.0%
	CACAACCCAAACAACAGAAT+AGG	+	Chr4:41995120-41995139	MsG0480020463.01.T01:CDS	40.0%
	CTTCAGCAGGATCAGAATAA+TGG	+	Chr4:41995306-41995325	MsG0480020463.01.T01:CDS	40.0%
	GTAAATAAACGGCGACCAAA+TGG	-	Chr4:41995247-41995266	None:intergenic	40.0%
	TATTGGAAGGGTTAAGTGGA+GGG	+	Chr4:41995353-41995372	MsG0480020463.01.T01:CDS	40.0%
	TGAACTGTCCTGCTTATGTT+AGG	+	Chr4:41995412-41995431	MsG0480020463.01.T01:CDS	40.0%
	TTATTGGAAGGGTTAAGTGG+AGG	+	Chr4:41995352-41995371	MsG0480020463.01.T01:CDS	40.0%
!	GCAGGATCAGAATAATGGAA+TGG	+	Chr4:41995311-41995330	MsG0480020463.01.T01:CDS	40.0%
	ACGCCAACGCCGTAAATAAA+CGG	-	Chr4:41995258-41995277	None:intergenic	45.0%
	CTTAGCGTAAAAGGCGAAAC+CGG	-	Chr4:41995282-41995301	None:intergenic	45.0%
	TACGCTAAGCAATCTTCAGC+AGG	+	Chr4:41995293-41995312	MsG0480020463.01.T01:CDS	45.0%
	TTAAGTGGAGGGTTGGAAGT+TGG	+	Chr4:41995364-41995383	MsG0480020463.01.T01:CDS	45.0%
!!	AAGTTGGTAGCGAGATCTGT+TGG	-	Chr4:41995188-41995207	None:intergenic	45.0%
	AGGGTTGGAAGTTGGATCTC+CGG	+	Chr4:41995372-41995391	MsG0480020463.01.T01:CDS	50.0%
	GCGGAGAACCTAACATAAGC+AGG	-	Chr4:41995423-41995442	None:intergenic	50.0%
	TATGTTAGGTTCTCCGCCTC+CGG	+	Chr4:41995426-41995445	MsG0480020463.01.T01:CDS	50.0%
	TCGCCGTTTATTTACGGCGT+TGG	+	Chr4:41995252-41995271	MsG0480020463.01.T01:CDS	50.0%
	TTGGAAGTTGGATCTCCGGA+AGG	+	Chr4:41995376-41995395	MsG0480020463.01.T01:CDS	50.0%
	ATTATTCCGGTCACCGGAGG+CGG	-	Chr4:41995442-41995461	None:intergenic	55.0%
	CAACTCCGGCGGCATTATTC+CGG	-	Chr4:41995455-41995474	None:intergenic	55.0%
	GGAAGGGTTAAGTGGAGGGT+TGG	+	Chr4:41995357-41995376	MsG0480020463.01.T01:CDS	55.0%
!!	TATTTACGGCGTTGGCGTGC+CGG	+	Chr4:41995260-41995279	MsG0480020463.01.T01:CDS	55.0%
	AACCGCCGGAGCAACAACTC+CGG	-	Chr4:41995469-41995488	None:intergenic	60.0%
	GGCATTATTCCGGTCACCGG+AGG	-	Chr4:41995445-41995464	None:intergenic	60.0%
	GGCGGCATTATTCCGGTCAC+CGG	-	Chr4:41995448-41995467	None:intergenic	60.0%
	GGTGACCGGAATAATGCCGC+CGG	+	Chr4:41995447-41995466	MsG0480020463.01.T01:CDS	60.0%
	TGCCGCCGGAGTTGTTGCTC+CGG	+	Chr4:41995461-41995480	MsG0480020463.01.T01:CDS	65.0%
	CGCCGGAGCAACAACTCCGG+CGG	-	Chr4:41995466-41995485	None:intergenic	70.0%
	CGCCGGAGTTGTTGCTCCGG+CGG	+	Chr4:41995464-41995483	MsG0480020463.01.T01:CDS	70.0%
	GGTTCTCCGCCTCCGGTGAC+CGG	+	Chr4:41995433-41995452	MsG0480020463.01.T01:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	41995099	41995527	41995099	ID=MsG0480020463.01;Name=MsG0480020463.01
Chr4	mRNA	41995099	41995527	41995099	ID=MsG0480020463.01.T01;Parent=MsG0480020463.01;Name=MsG0480020463.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|142
Chr4	exon	41995099	41995527	41995099	ID=MsG0480020463.01.T01:exon:4983;Parent=MsG0480020463.01.T01
Chr4	CDS	41995099	41995527	41995099	ID=MsG0480020463.01.T01:cds;Parent=MsG0480020463.01.T01

Gene Sequence

>MsG0480020463.01.T01

ATGCAAGTCACACTCACAACTCACAACCCAAACAACAGAATAGGCATCTACTATCAAAAACTCCATGTCTATGCTTCTTATAAGAGCCAACAGATCTCGCTACCAACTTCAATTCCAGATACTTACCAAGGTCATAAAGATTTTACCATTTGGTCGCCGTTTATTTACGGCGTTGGCGTGCCGGTTTCGCCTTTTACGCTAAGCAATCTTCAGCAGGATCAGAATAATGGAATGGTTTTGGTTAATATTAAGGTTATTGGAAGGGTTAAGTGGAGGGTTGGAAGTTGGATCTCCGGAAGGTATCATATTTTTGTGAACTGTCCTGCTTATGTTAGGTTCTCCGCCTCCGGTGACCGGAATAATGCCGCCGGAGTTGTTGCTCCGGCGGTTAAGTTTCAGCTTTTGCAAAGTTGTCATGTTGATGTTTAG

Protein sequence

>MsG0480020463.01.T01

MQVTLTTHNPNNRIGIYYQKLHVYASYKSQQISLPTSIPDTYQGHKDFTIWSPFIYGVGVPVSPFTLSNLQQDQNNGMVLVNIKVIGRVKWRVGSWISGRYHIFVNCPAYVRFSASGDRNNAAGVVAPAVKFQLLQSCHVDV*