AlfalfaGEDB | MsG0880042671.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042671.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042671.01.T01	RHN51428.1	33.902	410	197	11	1	360	1	386	2.30E-48	179

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042671.01.T01	A0A396HFP9	33.902	410	197	11	1	360	1	386	1.10e-48	179

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0080049033.01	MsG0880042671.01	PPI
MsG0180002283.01	MsG0880042671.01	PPI
MsG0180002283.01	MsG0880042671.01	PPI
MsG0180004482.01	MsG0880042671.01	PPI
MsG0180006100.01	MsG0880042671.01	PPI
MsG0180006260.01	MsG0880042671.01	PPI
MsG0280007632.01	MsG0880042671.01	PPI
MsG0280009289.01	MsG0880042671.01	PPI
MsG0380011614.01	MsG0880042671.01	PPI
MsG0380011614.01	MsG0880042671.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042671.01.T01	MTR_3g047490	34.226	336	157	7	1	285	1	323	3.32e-50	172
MsG0880042671.01.T01	MTR_6g039060	30.612	392	209	9	24	357	19	405	1.61e-46	167
MsG0880042671.01.T01	MTR_0132s0050	34.153	366	205	9	1	345	1	351	2.37e-45	163
MsG0880042671.01.T01	MTR_5g049460	31.941	407	201	12	24	375	19	404	4.46e-45	162
MsG0880042671.01.T01	MTR_0010s0130	41.148	209	114	3	24	226	19	224	4.11e-41	154
MsG0880042671.01.T01	MTR_6g052660	28.392	398	202	11	34	360	6	391	1.67e-37	143
MsG0880042671.01.T01	MTR_0752s0020	36.364	231	118	5	1	226	1	207	2.43e-37	143
MsG0880042671.01.T01	MTR_7g099763	39.196	199	114	3	26	217	24	222	1.02e-35	133
MsG0880042671.01.T01	MTR_5g084777	40.000	205	106	6	26	218	24	223	1.40e-35	139
MsG0880042671.01.T01	MTR_8g012072	38.725	204	110	4	26	218	24	223	2.92e-35	138

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 89 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGCTGGGTCAAAGAA+AGG	0.502080	8:+12384282	MsG0880042671.01.T01:CDS
AAAGAAAGGGAAAATGGTCA+TGG	0.567877	8:+12384296	MsG0880042671.01.T01:CDS
AAAGGAAAGATGCAGAAGGT+TGG	0.591290	8:+12385223	MsG0880042671.01.T01:CDS
AAAGTCGTAAGCAAACAAGC+TGG	0.548529	8:+12384270	MsG0880042671.01.T01:CDS
AACAAGCTGGGTCAAAGAAA+GGG	0.513083	8:+12384283	MsG0880042671.01.T01:CDS
AACATATGTGAACTTGGTCA+AGG	0.631522	8:+12384206	MsG0880042671.01.T01:CDS
AAGCAAAGCGTATCAGAATC+TGG	0.338583	8:+12384021	MsG0880042671.01.T01:CDS
AAGCCCCTAAAGATGAAAAG+TGG	0.558761	8:+12384882	MsG0880042671.01.T01:CDS
AAGGAAGATGTCCTGCTACA+AGG	0.568237	8:+12384453	MsG0880042671.01.T01:CDS
AAGTATGAAGTAAACATGGA+AGG	0.610824	8:+12384132	MsG0880042671.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTATGAAGTAAACATGGA+AGG	+	Chr8:12384132-12384151	MsG0880042671.01.T01:CDS	30.0%
AATTTACTTCATGGCTATTC+TGG	+	Chr8:12384623-12384642	MsG0880042671.01.T01:CDS	30.0%
AGATGATCCATTACATATCT+TGG	-	Chr8:12384662-12384681	None:intergenic	30.0%
GAGTATTTCAAGATGCTAAA+TGG	+	Chr8:12384180-12384199	MsG0880042671.01.T01:CDS	30.0%
GATGATCCATTACATATCTT+GGG	-	Chr8:12384661-12384680	None:intergenic	30.0%
TCTAAGCTATCAAATGATGT+AGG	-	Chr8:12384330-12384349	None:intergenic	30.0%
TGACTCTACTTGAACTATTA+AGG	-	Chr8:12384088-12384107	None:intergenic	30.0%
AAAGAAAGGGAAAATGGTCA+TGG	+	Chr8:12384296-12384315	MsG0880042671.01.T01:CDS	35.0%
AACATATGTGAACTTGGTCA+AGG	+	Chr8:12384206-12384225	MsG0880042671.01.T01:CDS	35.0%
AATAGTTCAAGTAGAGTCAC+CGG	+	Chr8:12384089-12384108	MsG0880042671.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	12383979	12385279	12383979	ID=MsG0880042671.01;Name=MsG0880042671.01
Chr8	mRNA	12383979	12385279	12383979	ID=MsG0880042671.01.T01;Parent=MsG0880042671.01;Name=MsG0880042671.01.T01;_AED=0.43;_eAED=1.00;_QI=0\|0\|0\|1\|0\|0\|2\|0\|392
Chr8	exon	12383979	12384667	12383979	ID=MsG0880042671.01.T01:exon:240;Parent=MsG0880042671.01.T01
Chr8	exon	12384790	12385279	12384790	ID=MsG0880042671.01.T01:exon:241;Parent=MsG0880042671.01.T01
Chr8	CDS	12383979	12384667	12383979	ID=MsG0880042671.01.T01:cds;Parent=MsG0880042671.01.T01
Chr8	CDS	12384790	12385279	12384790	ID=MsG0880042671.01.T01:cds;Parent=MsG0880042671.01.T01

Gene Sequence

>MsG0880042671.01.T01

ATGGCATCATCAAGCTCAAAAGTTTCAGAATCTACATCAAACAAGCAAAGCGTATCAGAATCTGGGTCCATCTACAAAGAGAAGACTATGGATATTCCATGGGAATCCTTAATAGTTCAAGTAGAGTCACCGGTGGATTTTGCTTCACTCAAGAAGTATGAAGTAAACATGGAAGGATTGATAGAGACTCAGATGCTGTCTGAGTATTTCAAGATGCTAAATGGGCCAACATATGTGAACTTGGTCAAGGACTTTTGGTTAAAAGCTGAAGTGTACAATGAAAAGCCTGCAAAAGTCGTAAGCAAACAAGCTGGGTCAAAGAAAGGGAAAATGGTCATGGATATGAATCCTACATCATTTGATAGCTTAGAAATAAGGTCTGAAGTGATGGGCATTCCTATAACCATCACTGAGGACGTTATAGCTAAAGCTTGTAGGATGGATGCAGAGGGAAGATTCCAAGAAATAGTCAGGAAGGAAGATGTCCTGCTACAAGGTTACTACAACACTCTTTTGGGTGGGAAGAAAGATGCAAAGCCTTCTGAAATGGAGATTCACAACAGGATGTTGGTGAAATTCAGTAATGATTGCTTCTTTCAAAGAGCAGGAGGATCTGACCAACCCAACAATCAACAGAAGCTAGCAATTTACTTCATGGCTATTCTGGAAAAGATAAATTCCCAAGATATGACAAGGAGTGCTTCTGATAGATGTTTCGAAGTCTCTACTGCTGAAAGGACTATCAGTAGCAGGAGCTTATGCTCTATGAAGTTCATCAAAGAAGCCCCTAAAGATGAAAAGTGGTTAGAGATGACAACTGCTGAAACTGAGATAATCAGGGACTTTCCTCCCATCTTTAAGGAAAGTGACTCTGATTTCCTAGTTCAATCAGTAGCTGAGTACGTCTTGGAGGAAGATGCGAGGTCTCAGGAGGAAGCTGCTCTTGCTAAAGGGAAAGGCAAGAAGGCCAAAGCAGTTGCTGTGTCAGAAGCTGCTGTTTCTGATGCACCTAAAGGGAAAAGGGCCAGAACTAGTTCTGAATCTGATGCTGCTCCTACTAAAGGAAAGAAGGCCAGAACTAGTACTGAGTCAGAAGCTGCTAAACCTGTTTCTATCAAGGAAAAAGGAAAGATGCAGAAGGTTGGTGCTGCTTCTATTCCAGAAGAGAAGAAAAGGTAA

Protein sequence

>MsG0880042671.01.T01

MASSSSKVSESTSNKQSVSESGSIYKEKTMDIPWESLIVQVESPVDFASLKKYEVNMEGLIETQMLSEYFKMLNGPTYVNLVKDFWLKAEVYNEKPAKVVSKQAGSKKGKMVMDMNPTSFDSLEIRSEVMGIPITITEDVIAKACRMDAEGRFQEIVRKEDVLLQGYYNTLLGGKKDAKPSEMEIHNRMLVKFSNDCFFQRAGGSDQPNNQQKLAIYFMAILEKINSQDMTRSASDRCFEVSTAERTISSRSLCSMKFIKEAPKDEKWLEMTTAETEIIRDFPPIFKESDSDFLVQSVAEYVLEEDARSQEEAALAKGKGKKAKAVAVSEAAVSDAPKGKRARTSSESDAAPTKGKKARTSTESEAAKPVSIKEKGKMQKVGAASIPEEKKR*