AlfalfaGEDB | MsG0880042728.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042728.01

KEGG: -
GO: GO:0030145 GO:0045735
PFAM: PF00190

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042728.01.T01	XP_003627277.1	96.172	209	8	0	1	209	1	209	5.82E-143	407

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042728.01.T01	Q9ZRA4	58.937	207	82	3	3	207	2	207	4.94E-79	238

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042728.01.T01	G7L6U9	96.172	209	8	0	1	209	1	209	2.78e-143	407

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000489.01	MsG0880042728.01	-0.800399	1.468103e-48	6.829056e-46
MsG0180000549.01	MsG0880042728.01	-0.808683	2.745331e-50	1.579724e-47
MsG0180000793.01	MsG0880042728.01	-0.803560	3.289183e-49	1.658169e-46
MsG0180002700.01	MsG0880042728.01	-0.800287	1.547266e-48	7.177009e-46
MsG0180003320.01	MsG0880042728.01	0.810297	1.236026e-50	7.422717e-48
MsG0180004059.01	MsG0880042728.01	0.801139	1.036852e-48	4.914597e-46
MsG0180004424.01	MsG0880042728.01	0.808653	2.786737e-50	1.602261e-47
MsG0180005130.01	MsG0880042728.01	0.852900	3.406900e-61	7.228113e-58
MsG0180005143.01	MsG0880042728.01	0.850527	1.602598e-60	3.148241e-57
MsG0180005760.01	MsG0880042728.01	-0.800212	1.602008e-48	7.416770e-46
MsG0180006195.01	MsG0880042728.01	0.803200	3.904111e-49	1.949951e-46
MsG0180006253.01	MsG0880042728.01	0.807566	4.748007e-50	2.653257e-47
MsG0780040343.01	MsG0880042728.01	-0.818486	1.911957e-52	1.433088e-49
MsG0780040684.01	MsG0880042728.01	-0.805691	1.181516e-49	6.291893e-47
MsG0780041004.01	MsG0880042728.01	-0.809069	2.270048e-50	1.319753e-47
MsG0780041289.01	MsG0880042728.01	-0.813284	2.766936e-51	1.799309e-48
MsG0780041385.01	MsG0880042728.01	-0.815280	1.002223e-51	6.880065e-49
MsG0780041778.01	MsG0880042728.01	-0.800234	1.586208e-48	7.347482e-46
MsG0880042728.01	MsG0880045412.01	-0.805686	1.184007e-49	6.304396e-47
MsG0880042728.01	MsG0880045801.01	0.811599	6.460415e-51	4.015800e-48
MsG0880042728.01	MsG0880046252.01	-0.804174	2.451608e-49	1.255506e-46
MsG0880042728.01	MsG0880046434.01	0.828438	8.990826e-55	8.936764e-52
MsG0880042728.01	MsG0880046759.01	-0.801835	7.463098e-49	3.600155e-46
MsG0880042728.01	MsG0880046791.01	-0.827357	1.636196e-54	1.576060e-51
MsG0880042728.01	MsG0880047312.01	0.843729	1.171291e-58	1.849461e-55
MsG0880042728.01	MsG0880047460.01	0.805116	1.559110e-49	8.180615e-47
MsG0880042728.01	MsG0880047727.01	-0.802195	6.295621e-49	3.064762e-46
MsG0280010623.01	MsG0880042728.01	0.817789	2.748464e-52	2.020961e-49
MsG0280011110.01	MsG0880042728.01	0.812278	4.595386e-51	2.909403e-48
MsG0380011662.01	MsG0880042728.01	-0.811886	5.593982e-51	3.503834e-48
MsG0380013212.01	MsG0880042728.01	0.820007	8.610231e-53	6.732137e-50
MsG0380013213.01	MsG0880042728.01	0.834271	3.300451e-56	3.897319e-53
MsG0380013530.01	MsG0880042728.01	-0.819583	1.076441e-52	8.317225e-50
MsG0380014909.01	MsG0880042728.01	-0.815442	9.226585e-52	6.362121e-49
MsG0680031200.01	MsG0880042728.01	0.801539	8.585698e-49	4.110653e-46
MsG0680032026.01	MsG0880042728.01	0.809342	1.984529e-50	1.162215e-47
MsG0680032515.01	MsG0880042728.01	-0.808267	3.368281e-50	1.917383e-47
MsG0680034974.01	MsG0880042728.01	0.808578	2.890454e-50	1.658728e-47
MsG0680034980.01	MsG0880042728.01	0.827312	1.677447e-54	1.613538e-51
MsG0680034981.01	MsG0880042728.01	0.800126	1.668007e-48	7.705532e-46
MsG0480018617.01	MsG0880042728.01	0.834439	2.995330e-56	3.554845e-53
MsG0480018621.01	MsG0880042728.01	0.833236	5.986940e-56	6.853151e-53
MsG0480018692.01	MsG0880042728.01	0.817676	2.913738e-52	2.135844e-49
MsG0480018693.01	MsG0880042728.01	0.827891	1.217503e-54	1.190999e-51
MsG0480018697.01	MsG0880042728.01	0.808414	3.134244e-50	1.790950e-47
MsG0480019322.01	MsG0880042728.01	0.845721	3.402521e-59	5.722885e-56
MsG0480019641.01	MsG0880042728.01	-0.800776	1.229672e-48	5.774843e-46
MsG0480020285.01	MsG0880042728.01	0.822795	1.956432e-53	1.654233e-50
MsG0480020753.01	MsG0880042728.01	-0.811664	6.251659e-51	3.892696e-48
MsG0480021091.01	MsG0880042728.01	0.842316	2.785150e-58	4.206967e-55
MsG0480021101.01	MsG0880042728.01	-0.806368	8.509826e-50	4.610596e-47
MsG0480021682.01	MsG0880042728.01	-0.809152	2.179455e-50	1.269915e-47
MsG0580024944.01	MsG0880042728.01	-0.814833	1.259530e-51	8.541704e-49
MsG0580024994.01	MsG0880042728.01	0.800815	1.207169e-48	5.674810e-46
MsG0580025007.01	MsG0880042728.01	0.814687	1.356941e-51	9.166328e-49
MsG0580025024.01	MsG0880042728.01	0.834450	2.976116e-56	3.533294e-53
MsG0580025589.01	MsG0880042728.01	-0.814538	1.463790e-51	9.848673e-49
MsG0380016452.01	MsG0880042728.01	-0.834041	3.769042e-56	4.419822e-53
MsG0380016836.01	MsG0880042728.01	-0.825701	4.060820e-54	3.729277e-51
MsG0380017739.01	MsG0880042728.01	-0.808884	2.486947e-50	1.438640e-47
MsG0380017976.01	MsG0880042728.01	-0.815388	9.483603e-52	6.529565e-49
MsG0580026521.01	MsG0880042728.01	0.803973	2.698886e-49	1.375093e-46
MsG0580027751.01	MsG0880042728.01	0.805346	1.395792e-49	7.366937e-47
MsG0580027777.01	MsG0880042728.01	0.808858	2.518957e-50	1.456176e-47
MsG0580028306.01	MsG0880042728.01	-0.816746	4.717785e-52	3.371198e-49
MsG0280006706.01	MsG0880042728.01	-0.810510	1.111938e-50	6.715395e-48
MsG0280007513.01	MsG0880042728.01	0.849444	3.220710e-60	6.106616e-57
MsG0280007677.01	MsG0880042728.01	0.840994	6.218707e-58	9.011678e-55
MsG0280007689.01	MsG0880042728.01	0.811484	6.841943e-51	4.240423e-48
MsG0280007874.01	MsG0880042728.01	0.802553	5.311023e-49	2.609192e-46
MsG0280008178.01	MsG0880042728.01	0.800413	1.458353e-48	6.786363e-46
MsG0280008180.01	MsG0880042728.01	0.811901	5.552546e-51	3.479314e-48
MsG0280008227.01	MsG0880042728.01	-0.807294	5.423857e-50	3.009730e-47
MsG0780035932.01	MsG0880042728.01	0.833008	6.824734e-56	7.759416e-53
MsG0780036258.01	MsG0880042728.01	0.820814	5.620751e-53	4.494181e-50
MsG0780036259.01	MsG0880042728.01	0.830499	2.837884e-55	2.995056e-52
MsG0780036260.01	MsG0880042728.01	0.824347	8.478957e-54	7.490291e-51
MsG0780036835.01	MsG0880042728.01	0.800083	1.702188e-48	7.854809e-46
MsG0780037436.01	MsG0880042728.01	-0.815202	1.043347e-51	7.146497e-49
MsG0780039449.01	MsG0880042728.01	-0.806377	8.472486e-50	4.591430e-47
MsG0780039686.01	MsG0880042728.01	0.811165	8.023564e-51	4.930886e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042728.01.T01	MTR_8g020630	96.172	209	8	0	1	209	1	209	7.04e-147	407
MsG0880042728.01.T01	MTR_8g020650	95.694	209	9	0	1	209	1	209	3.41e-138	385
MsG0880042728.01.T01	MTR_8g020670	89.474	209	22	0	1	209	1	209	5.85e-136	379
MsG0880042728.01.T01	MTR_2g044040	74.641	209	53	0	1	209	1	209	2.12e-113	322
MsG0880042728.01.T01	MTR_0284s0010	74.163	209	54	0	1	209	1	209	2.49e-110	315
MsG0880042728.01.T01	MTR_2g045100	71.292	209	60	0	1	209	1	209	1.48e-107	308
MsG0880042728.01.T01	MTR_2g036120	71.014	207	60	0	1	207	1	207	1.51e-106	305
MsG0880042728.01.T01	MTR_3g019710	70.813	209	61	0	1	209	1	209	1.44e-105	303
MsG0880042728.01.T01	MTR_5g031460	68.900	209	65	0	1	209	1	209	3.26e-102	294
MsG0880042728.01.T01	MTR_3g019650	68.900	209	64	1	1	209	1	208	2.87e-100	289
MsG0880042728.01.T01	MTR_3g019680	68.900	209	64	1	1	209	1	208	9.06e-100	288
MsG0880042728.01.T01	MTR_7g011340	70.813	209	61	0	1	209	1	209	4.28e-98	283
MsG0880042728.01.T01	MTR_4g010340	61.538	208	76	3	3	209	1	205	5.38e-86	253
MsG0880042728.01.T01	MTR_3g019810	64.532	203	66	1	1	203	107	303	1.40e-84	253
MsG0880042728.01.T01	MTR_8g020590	57.416	209	86	3	3	209	1	208	3.13e-81	241
MsG0880042728.01.T01	MTR_2g041550	56.938	209	86	3	3	209	4	210	4.97e-80	238
MsG0880042728.01.T01	MTR_8g020760	56.398	211	90	2	1	209	1	211	8.13e-80	237
MsG0880042728.01.T01	MTR_2g019780	55.814	215	86	3	1	209	1	212	8.78e-80	237
MsG0880042728.01.T01	MTR_3g073170	56.338	213	86	3	1	209	1	210	1.36e-79	237
MsG0880042728.01.T01	MTR_2g041580	55.981	209	88	3	3	209	4	210	5.21e-79	235
MsG0880042728.01.T01	MTR_8g020610	57.416	209	85	3	3	209	1	207	1.45e-78	234
MsG0880042728.01.T01	MTR_2g041570	55.502	209	89	3	3	209	4	210	3.56e-78	233
MsG0880042728.01.T01	MTR_8g020770	53.774	212	95	3	1	209	1	212	1.29e-77	232
MsG0880042728.01.T01	MTR_8g020750	53.774	212	95	3	1	209	1	212	1.59e-73	221
MsG0880042728.01.T01	MTR_8g020763	58.242	182	75	1	29	209	9	190	2.37e-72	218
MsG0880042728.01.T01	MTR_4g027415	73.239	142	38	0	1	142	1	142	4.12e-64	195
MsG0880042728.01.T01	MTR_2g072560	66.957	115	38	0	1	115	1	115	1.52e-52	166
MsG0880042728.01.T01	MTR_3g019610	70.755	106	31	0	1	106	1	106	1.33e-49	157
MsG0880042728.01.T01	MTR_1g085140	36.181	199	123	3	14	208	20	218	3.29e-37	129
MsG0880042728.01.T01	MTR_7g111240	38.021	192	115	3	21	208	23	214	3.35e-37	129
MsG0880042728.01.T01	MTR_7g111240	38.021	192	112	4	21	208	23	211	2.63e-36	126
MsG0880042728.01.T01	MTR_1g085500	34.450	209	131	4	6	208	9	217	5.81e-36	126
MsG0880042728.01.T01	MTR_1g112200	35.437	206	124	5	3	205	8	207	1.50e-35	124
MsG0880042728.01.T01	MTR_2g031270	36.453	203	116	5	9	205	18	213	6.74e-34	120
MsG0880042728.01.T01	MTR_1g060120	33.168	202	127	2	12	205	20	221	9.33e-34	120
MsG0880042728.01.T01	MTR_2g030855	36.041	197	117	4	15	205	19	212	1.06e-33	120
MsG0880042728.01.T01	MTR_1g079490	34.579	214	126	4	3	205	7	217	3.11e-33	119
MsG0880042728.01.T01	MTR_4g017050	36.239	218	122	6	1	204	1	215	8.84e-33	118
MsG0880042728.01.T01	MTR_5g046410	36.239	218	122	6	1	204	1	215	1.22e-32	117
MsG0880042728.01.T01	MTR_5g046430	36.239	218	122	6	1	204	1	215	1.22e-32	117
MsG0880042728.01.T01	MTR_4g017030	36.239	218	122	6	1	204	1	215	1.22e-32	117
MsG0880042728.01.T01	MTR_2g019250	35.780	218	123	6	1	204	1	215	2.47e-32	117
MsG0880042728.01.T01	MTR_6g005310	38.119	202	111	5	14	205	18	215	2.54e-32	116
MsG0880042728.01.T01	MTR_6g005330	36.986	219	120	6	1	205	1	215	2.65e-32	116
MsG0880042728.01.T01	MTR_6g005360	38.119	202	111	5	14	205	18	215	2.95e-32	116
MsG0880042728.01.T01	MTR_4g017040	35.185	216	127	5	1	204	1	215	5.58e-32	115
MsG0880042728.01.T01	MTR_6g005340	37.624	202	112	5	14	205	18	215	1.44e-31	114
MsG0880042728.01.T01	MTR_1g023770	33.010	206	126	4	6	205	10	209	2.66e-31	114
MsG0880042728.01.T01	MTR_6g005380	36.979	192	112	4	11	194	15	205	1.61e-28	106
MsG0880042728.01.T01	MTR_2g030895	35.465	172	104	4	41	205	7	178	9.69e-28	103
MsG0880042728.01.T01	MTR_8g007300	35.028	177	100	5	4	169	8	180	2.86e-26	101
MsG0880042728.01.T01	MTR_2g030865	35.465	172	104	4	41	205	7	178	4.04e-26	99.8
MsG0880042728.01.T01	MTR_3g096330	34.503	171	103	2	8	169	561	731	4.47e-26	105
MsG0880042728.01.T01	MTR_5g036570	31.414	191	126	4	22	207	31	221	1.68e-25	99.0
MsG0880042728.01.T01	MTR_6g005350	33.333	201	127	4	11	205	15	214	5.80e-25	97.8
MsG0880042728.01.T01	MTR_2g086630	38.994	159	91	4	52	205	47	204	3.17e-24	95.5
MsG0880042728.01.T01	MTR_2g086620	38.365	159	92	4	52	205	47	204	1.62e-23	93.6
MsG0880042728.01.T01	MTR_2g031300	44.340	106	55	3	41	142	7	112	1.17e-21	87.4
MsG0880042728.01.T01	MTR_2g086640	37.107	159	94	4	52	205	46	203	2.30e-21	87.8
MsG0880042728.01.T01	MTR_2g096110	35.915	142	74	3	40	178	2	129	3.40e-19	80.5
MsG0880042728.01.T01	MTR_4g052770	36.296	135	84	2	73	205	71	205	5.89e-19	81.6
MsG0880042728.01.T01	MTR_4g052780	36.296	135	84	2	73	205	71	205	1.16e-18	80.9
MsG0880042728.01.T01	MTR_7g029500	52.830	53	25	0	150	202	38	90	2.08e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042728.01.T01	AT1G72610	60.622	193	74	2	19	209	16	208	6.10e-78	233
MsG0880042728.01.T01	AT5G20630	56.522	207	87	3	3	207	4	209	3.40e-76	228
MsG0880042728.01.T01	AT5G39160	40.196	204	110	5	12	206	16	216	4.60e-41	139
MsG0880042728.01.T01	AT5G39190	40.291	206	111	6	12	206	16	220	3.51e-40	137
MsG0880042728.01.T01	AT5G39160	39.806	206	112	6	12	206	16	220	1.03e-39	135
MsG0880042728.01.T01	AT3G62020	36.493	211	124	5	8	208	8	218	1.83e-39	135
MsG0880042728.01.T01	AT5G39130	40.291	206	111	6	12	206	16	220	1.99e-39	135
MsG0880042728.01.T01	AT3G05950	41.969	193	102	5	22	205	30	221	2.00e-39	135
MsG0880042728.01.T01	AT3G04200	39.378	193	109	5	22	206	31	223	5.75e-39	134
MsG0880042728.01.T01	AT5G26700	37.198	207	126	4	5	208	7	212	7.60e-39	133
MsG0880042728.01.T01	AT1G09560	40.957	188	107	3	22	205	28	215	2.39e-38	132
MsG0880042728.01.T01	AT5G39110	38.182	220	121	5	1	205	1	220	3.24e-38	132
MsG0880042728.01.T01	AT1G18980	36.765	204	123	4	6	205	17	218	3.65e-38	132
MsG0880042728.01.T01	AT1G02335	35.681	213	129	4	4	208	6	218	8.78e-38	130
MsG0880042728.01.T01	AT3G05930	37.441	211	124	4	3	205	5	215	9.37e-38	130
MsG0880042728.01.T01	AT5G39180	37.727	220	121	6	1	205	1	219	3.06e-37	129
MsG0880042728.01.T01	AT5G39120	37.727	220	121	6	1	205	1	219	7.80e-37	128
MsG0880042728.01.T01	AT5G39150	37.727	220	121	6	1	205	1	219	9.26e-37	128
MsG0880042728.01.T01	AT3G62020	38.418	177	104	4	37	208	13	189	1.94e-36	126
MsG0880042728.01.T01	AT5G39190	40.449	178	98	3	37	206	21	198	1.41e-35	124
MsG0880042728.01.T01	AT5G39160	39.888	178	99	3	37	206	21	198	5.02e-35	123
MsG0880042728.01.T01	AT5G38960	35.266	207	117	5	13	206	21	223	4.26e-34	121
MsG0880042728.01.T01	AT1G18970	35.821	201	123	4	9	205	20	218	1.40e-33	120
MsG0880042728.01.T01	AT3G04150	35.204	196	119	4	22	209	30	225	3.23e-33	119
MsG0880042728.01.T01	AT3G10080	38.265	196	103	7	22	205	30	219	3.28e-33	119
MsG0880042728.01.T01	AT3G10080	38.265	196	103	7	22	205	101	290	1.52e-32	119
MsG0880042728.01.T01	AT3G04150	35.556	180	109	3	37	209	59	238	1.82e-32	117
MsG0880042728.01.T01	AT4G14630	35.437	206	116	5	13	205	21	222	3.90e-32	116
MsG0880042728.01.T01	AT1G74820	36.559	186	112	4	24	205	42	225	2.27e-31	114
MsG0880042728.01.T01	AT5G38940	38.144	194	112	5	20	205	28	221	3.51e-31	114
MsG0880042728.01.T01	AT5G38940	38.144	194	112	5	20	205	52	245	5.94e-31	114
MsG0880042728.01.T01	AT5G38910	35.484	217	124	5	1	205	7	219	6.22e-31	113
MsG0880042728.01.T01	AT5G61750	37.931	174	98	4	4	170	5	175	2.11e-30	111
MsG0880042728.01.T01	AT5G38930	36.000	200	120	5	14	205	22	221	1.20e-29	110
MsG0880042728.01.T01	AT3G04190	32.161	199	127	4	15	205	23	221	1.33e-29	109
MsG0880042728.01.T01	AT3G04170	33.803	213	122	7	6	205	12	218	6.53e-29	108
MsG0880042728.01.T01	AT3G04180	32.161	199	127	4	15	205	23	221	1.15e-27	104
MsG0880042728.01.T01	AT1G10460	32.222	180	93	4	7	183	38	191	1.44e-19	83.6
MsG0880042728.01.T01	AT5G38910	28.972	214	105	5	1	205	7	182	1.02e-15	72.4

Find 47 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAAGAGTTGTTGGTGTTTC+CGG	0.235370	8:-13275181	None:intergenic
CAATTCAGAAGGTAAATTAT+TGG	0.292227	8:-13275541	None:intergenic
GTTGAAACCGGGCGATCTTA+TGG	0.295038	8:+13275401	MsG0880042728.01.T01:CDS
CAATAATTTACCTTCTGAAT+TGG	0.309787	8:+13275542	MsG0880042728.01.T01:CDS
TGATAGCCTGAAGGGGTATC+TGG	0.313327	8:-13275102	None:intergenic
TTATTCTAAGACGTTGAAAC+CGG	0.377736	8:+13275389	MsG0880042728.01.T01:CDS
TTTCCAGGCCTTAACGGACT+TGG	0.385891	8:+13275237	MsG0880042728.01.T01:CDS
GTCTTTCACGGCTTCGTAGC+CGG	0.397464	8:+13275162	MsG0880042728.01.T01:CDS
TTGAACTATTATCAATAATT+CGG	0.400836	8:-13275328	None:intergenic
CAGGCTCCAGATACCCCTTC+AGG	0.418942	8:+13275096	MsG0880042728.01.T01:CDS
TCTTCACTTGTGCAAGATCA+AGG	0.425262	8:-13275581	None:intergenic
AAGAAGCTAAAGGCACGTTT+TGG	0.429524	8:+13275600	MsG0880042728.01.T01:CDS
TAATTTACCTTCTGAATTGG+TGG	0.446679	8:+13275545	MsG0880042728.01.T01:CDS
AAAATCATTGACAGAAGCTT+GGG	0.456575	8:-13275058	None:intergenic
TCAGACGATTTCGTCTTTCA+CGG	0.473366	8:+13275150	MsG0880042728.01.T01:CDS
CGGAGATTCCAAGTCCGTTA+AGG	0.474170	8:-13275245	None:intergenic
TTTCAAATTAACACTGGTAA+GGG	0.484943	8:+13275447	MsG0880042728.01.T01:CDS
GTCTGATGTGATATTTGCTA+GGG	0.486410	8:-13275133	None:intergenic
CGTCTGATGTGATATTTGCT+AGG	0.500974	8:-13275134	None:intergenic
GAATTATTGATAATAGTTCA+AGG	0.514232	8:+13275330	MsG0880042728.01.T01:CDS
TGCGGCGTTGAAAGAGTTGT+TGG	0.517226	8:-13275190	None:intergenic
ATTCCAAGTCCGTTAAGGCC+TGG	0.517650	8:-13275240	None:intergenic
GTTCAAGGTGAAATAACTGC+TGG	0.522312	8:+13275345	MsG0880042728.01.T01:CDS
AGATCAAGTAATTGAGCACC+AGG	0.524185	8:-13275507	None:intergenic
ATCAATAATTCGGTAGCACC+AGG	0.528604	8:-13275318	None:intergenic
ATTCCAATGCATACTCATCC+TGG	0.533481	8:+13275300	MsG0880042728.01.T01:CDS
TATTCTAAGACGTTGAAACC+GGG	0.536714	8:+13275390	MsG0880042728.01.T01:CDS
GCACCAGGATGAGTATGCAT+TGG	0.553990	8:-13275303	None:intergenic
GTTTGCAGTGATAGCCTGAA+GGG	0.577336	8:-13275110	None:intergenic
GTTTGTGCCACCAATTCAGA+AGG	0.585911	8:-13275552	None:intergenic
TGAAAATGCAACATCCCTTG+TGG	0.593070	8:-13275429	None:intergenic
CGCTATGTCTAACCGCGCTG+CGG	0.604451	8:-13275265	None:intergenic
TGCACAAGTGAAGAAGCTAA+AGG	0.608175	8:+13275590	MsG0880042728.01.T01:CDS
GGTTTGCAGTGATAGCCTGA+AGG	0.608817	8:-13275111	None:intergenic
GACGAATGCGGAGGTAAGTG+CGG	0.628119	8:-13275208	None:intergenic
TGGATTTCTGACACCTACCG+CGG	0.639675	8:+13275365	MsG0880042728.01.T01:CDS
TCTGATGTGATATTTGCTAG+GGG	0.642281	8:-13275132	None:intergenic
TGGAAAATCAGTGACGAATG+CGG	0.643979	8:-13275220	None:intergenic
CGGTTAGACATAGCGGAAGG+TGG	0.644542	8:+13275273	MsG0880042728.01.T01:CDS
GTCTTAGAATAAACCGCGGT+AGG	0.649666	8:-13275378	None:intergenic
CGCAGCGCGGTTAGACATAG+CGG	0.655117	8:+13275266	MsG0880042728.01.T01:CDS
GCGCGGTTAGACATAGCGGA+AGG	0.662984	8:+13275270	MsG0880042728.01.T01:CDS
TTTGCAGTGATAGCCTGAAG+GGG	0.694847	8:-13275109	None:intergenic
AAAATCAGTGACGAATGCGG+AGG	0.711598	8:-13275217	None:intergenic
GATCAAGTAATTGAGCACCA+GGG	0.730668	8:-13275506	None:intergenic
CAACGTCTTAGAATAAACCG+CGG	0.742395	8:-13275382	None:intergenic
GACTTGGAATCTCCGCAGCG+CGG	0.748357	8:+13275253	MsG0880042728.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAACTATTATCAATAATT+CGG	-	Chr8:13275331-13275350	None:intergenic	15.0%
!!!	GAATTATTGATAATAGTTCA+AGG	+	Chr8:13275330-13275349	MsG0880042728.01.T01:CDS	20.0%
!	CAATAATTTACCTTCTGAAT+TGG	+	Chr8:13275542-13275561	MsG0880042728.01.T01:CDS	25.0%
!	CAATTCAGAAGGTAAATTAT+TGG	-	Chr8:13275544-13275563	None:intergenic	25.0%
!	TTTCAAATTAACACTGGTAA+GGG	+	Chr8:13275447-13275466	MsG0880042728.01.T01:CDS	25.0%
!!	TTGCATTTTCAAATTAACAC+TGG	+	Chr8:13275441-13275460	MsG0880042728.01.T01:CDS	25.0%
!!	TTTTCAAATTAACACTGGTA+AGG	+	Chr8:13275446-13275465	MsG0880042728.01.T01:CDS	25.0%
!!	TTTTTGTGTTGCAAATTTAC+AGG	+	Chr8:13275077-13275096	MsG0880042728.01.T01:CDS	25.0%
	AAAAATCATTGACAGAAGCT+TGG	-	Chr8:13275062-13275081	None:intergenic	30.0%
	AAAATCATTGACAGAAGCTT+GGG	-	Chr8:13275061-13275080	None:intergenic	30.0%
	TAATTTACCTTCTGAATTGG+TGG	+	Chr8:13275545-13275564	MsG0880042728.01.T01:CDS	30.0%
!	ATCTTATGGTTTTTCCACAA+GGG	+	Chr8:13275415-13275434	MsG0880042728.01.T01:CDS	30.0%
!!	TTATTCTAAGACGTTGAAAC+CGG	+	Chr8:13275389-13275408	MsG0880042728.01.T01:CDS	30.0%
!	GATCTTATGGTTTTTCCACA+AGG	+	Chr8:13275414-13275433	MsG0880042728.01.T01:CDS	35.0%
!	GTCTGATGTGATATTTGCTA+GGG	-	Chr8:13275136-13275155	None:intergenic	35.0%
!	TCTGATGTGATATTTGCTAG+GGG	-	Chr8:13275135-13275154	None:intergenic	35.0%
!!	TATTCTAAGACGTTGAAACC+GGG	+	Chr8:13275390-13275409	MsG0880042728.01.T01:CDS	35.0%
	AGATCAAGTAATTGAGCACC+AGG	-	Chr8:13275510-13275529	None:intergenic	40.0%
	ATCAATAATTCGGTAGCACC+AGG	-	Chr8:13275321-13275340	None:intergenic	40.0%
	ATTCCAATGCATACTCATCC+TGG	+	Chr8:13275300-13275319	MsG0880042728.01.T01:CDS	40.0%
	CAACGTCTTAGAATAAACCG+CGG	-	Chr8:13275385-13275404	None:intergenic	40.0%
	CGTCTGATGTGATATTTGCT+AGG	-	Chr8:13275137-13275156	None:intergenic	40.0%
	GATCAAGTAATTGAGCACCA+GGG	-	Chr8:13275509-13275528	None:intergenic	40.0%
	GTTCAAGGTGAAATAACTGC+TGG	+	Chr8:13275345-13275364	MsG0880042728.01.T01:CDS	40.0%
	TCAGACGATTTCGTCTTTCA+CGG	+	Chr8:13275150-13275169	MsG0880042728.01.T01:CDS	40.0%
	TCTTCACTTGTGCAAGATCA+AGG	-	Chr8:13275584-13275603	None:intergenic	40.0%
	TGAAAATGCAACATCCCTTG+TGG	-	Chr8:13275432-13275451	None:intergenic	40.0%
	TGCACAAGTGAAGAAGCTAA+AGG	+	Chr8:13275590-13275609	MsG0880042728.01.T01:CDS	40.0%
	TGGAAAATCAGTGACGAATG+CGG	-	Chr8:13275223-13275242	None:intergenic	40.0%
!	ACTTTTAGTAGTGCTAACCC+TGG	+	Chr8:13275489-13275508	MsG0880042728.01.T01:CDS	40.0%
!	GAAAGAGTTGTTGGTGTTTC+CGG	-	Chr8:13275184-13275203	None:intergenic	40.0%
!!	AAGAAGCTAAAGGCACGTTT+TGG	+	Chr8:13275600-13275619	MsG0880042728.01.T01:CDS	40.0%
!!	ACTGATTTTCCAGGCCTTAA+CGG	+	Chr8:13275231-13275250	MsG0880042728.01.T01:CDS	40.0%
	AAAATCAGTGACGAATGCGG+AGG	-	Chr8:13275220-13275239	None:intergenic	45.0%
	GGAAAAACCATAAGATCGCC+CGG	-	Chr8:13275411-13275430	None:intergenic	45.0%
	GTCTTAGAATAAACCGCGGT+AGG	-	Chr8:13275381-13275400	None:intergenic	45.0%
	GTTTGCAGTGATAGCCTGAA+GGG	-	Chr8:13275113-13275132	None:intergenic	45.0%
	GTTTGTGCCACCAATTCAGA+AGG	-	Chr8:13275555-13275574	None:intergenic	45.0%
	TTTGCAGTGATAGCCTGAAG+GGG	-	Chr8:13275112-13275131	None:intergenic	45.0%
!!	AAGCTAAAGGCACGTTTTGG+TGG	+	Chr8:13275603-13275622	MsG0880042728.01.T01:CDS	45.0%
!!!	GCATTCGTCACTGATTTTCC+AGG	+	Chr8:13275222-13275241	MsG0880042728.01.T01:CDS	45.0%
	ATTCCAAGTCCGTTAAGGCC+TGG	-	Chr8:13275243-13275262	None:intergenic	50.0%
	CGGAGATTCCAAGTCCGTTA+AGG	-	Chr8:13275248-13275267	None:intergenic	50.0%
	GCACCAGGATGAGTATGCAT+TGG	-	Chr8:13275306-13275325	None:intergenic	50.0%
	GGTTTGCAGTGATAGCCTGA+AGG	-	Chr8:13275114-13275133	None:intergenic	50.0%
	GTTGAAACCGGGCGATCTTA+TGG	+	Chr8:13275401-13275420	MsG0880042728.01.T01:CDS	50.0%
	TGGATTTCTGACACCTACCG+CGG	+	Chr8:13275365-13275384	MsG0880042728.01.T01:CDS	50.0%
!	TGCGGCGTTGAAAGAGTTGT+TGG	-	Chr8:13275193-13275212	None:intergenic	50.0%
!!	AAGGCACGTTTTGGTGGAAG+AGG	+	Chr8:13275609-13275628	MsG0880042728.01.T01:CDS	50.0%
!!	AGGCACGTTTTGGTGGAAGA+GGG	+	Chr8:13275610-13275629	MsG0880042728.01.T01:CDS	50.0%
!!	TGATAGCCTGAAGGGGTATC+TGG	-	Chr8:13275105-13275124	None:intergenic	50.0%
!!	TTTCCAGGCCTTAACGGACT+TGG	+	Chr8:13275237-13275256	MsG0880042728.01.T01:CDS	50.0%
	GACGAATGCGGAGGTAAGTG+CGG	-	Chr8:13275211-13275230	None:intergenic	55.0%
	GTCTTTCACGGCTTCGTAGC+CGG	+	Chr8:13275162-13275181	MsG0880042728.01.T01:CDS	55.0%
!	CGGTTAGACATAGCGGAAGG+TGG	+	Chr8:13275273-13275292	MsG0880042728.01.T01:CDS	55.0%
	CAGGCTCCAGATACCCCTTC+AGG	+	Chr8:13275096-13275115	MsG0880042728.01.T01:CDS	60.0%
	CGCAGCGCGGTTAGACATAG+CGG	+	Chr8:13275266-13275285	MsG0880042728.01.T01:CDS	60.0%
	CGCTATGTCTAACCGCGCTG+CGG	-	Chr8:13275268-13275287	None:intergenic	60.0%
	GACTTGGAATCTCCGCAGCG+CGG	+	Chr8:13275253-13275272	MsG0880042728.01.T01:CDS	60.0%
!	GCGCGGTTAGACATAGCGGA+AGG	+	Chr8:13275270-13275289	MsG0880042728.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	13275006	13275635	13275006	ID=MsG0880042728.01;Name=MsG0880042728.01
Chr8	mRNA	13275006	13275635	13275006	ID=MsG0880042728.01.T01;Parent=MsG0880042728.01;Name=MsG0880042728.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|209
Chr8	exon	13275006	13275635	13275006	ID=MsG0880042728.01.T01:exon:36777;Parent=MsG0880042728.01.T01
Chr8	CDS	13275006	13275635	13275006	ID=MsG0880042728.01.T01:cds;Parent=MsG0880042728.01.T01

Gene Sequence

>MsG0880042728.01.T01

ATGAAGATTATTCACATAGTTTTTCTTTTCACTATTCTTTCGTTCACTACTTCCCAAGCTTCTGTCAATGATTTTTGTGTTGCAAATTTACAGGCTCCAGATACCCCTTCAGGCTATCACTGCAAACCCCTAGCAAATATCACATCAGACGATTTCGTCTTTCACGGCTTCGTAGCCGGAAACACCAACAACTCTTTCAACGCCGCACTTACCTCCGCATTCGTCACTGATTTTCCAGGCCTTAACGGACTTGGAATCTCCGCAGCGCGGTTAGACATAGCGGAAGGTGGTTCAATTCCAATGCATACTCATCCTGGTGCTACCGAATTATTGATAATAGTTCAAGGTGAAATAACTGCTGGATTTCTGACACCTACCGCGGTTTATTCTAAGACGTTGAAACCGGGCGATCTTATGGTTTTTCCACAAGGGATGTTGCATTTTCAAATTAACACTGGTAAGGGAAAAGCTACTGCTTTTCTTACTTTTAGTAGTGCTAACCCTGGTGCTCAATTACTTGATCTTCTTTTGTTTTCCAATAATTTACCTTCTGAATTGGTGGCACAAACTACTTTCCTTGATCTTGCACAAGTGAAGAAGCTAAAGGCACGTTTTGGTGGAAGAGGGTAG

Protein sequence

>MsG0880042728.01.T01

MKIIHIVFLFTILSFTTSQASVNDFCVANLQAPDTPSGYHCKPLANITSDDFVFHGFVAGNTNNSFNAALTSAFVTDFPGLNGLGISAARLDIAEGGSIPMHTHPGATELLIIVQGEITAGFLTPTAVYSKTLKPGDLMVFPQGMLHFQINTGKGKATAFLTFSSANPGAQLLDLLLFSNNLPSELVAQTTFLDLAQVKKLKARFGGRG*