Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043842.01.T01 | XP_003628077.1 | 83.582 | 469 | 67 | 4 | 1 | 468 | 1 | 460 | 0 | 803 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043842.01.T01 | Q9SD53 | 23.427 | 461 | 279 | 16 | 26 | 465 | 48 | 455 | 1.61E-19 | 94.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043842.01.T01 | G7LJ67 | 83.582 | 469 | 67 | 4 | 1 | 468 | 1 | 460 | 0.0 | 803 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780040254.01 | MsG0880043842.01 | 0.837920 | 3.908502e-57 | 5.152988e-54 |
| MsG0880043480.01 | MsG0880043842.01 | 0.801625 | 8.244656e-49 | 3.955777e-46 |
| MsG0880043522.01 | MsG0880043842.01 | 0.803387 | 3.571604e-49 | 1.792418e-46 |
| MsG0280010816.01 | MsG0880043842.01 | 0.803163 | 3.973670e-49 | 1.982859e-46 |
| MsG0380012020.01 | MsG0880043842.01 | 0.831260 | 1.846288e-55 | 1.992797e-52 |
| MsG0480023873.01 | MsG0880043842.01 | 0.802487 | 5.481575e-49 | 2.688415e-46 |
| MsG0480024014.01 | MsG0880043842.01 | 0.814104 | 1.825992e-51 | 1.214141e-48 |
| MsG0380017375.01 | MsG0880043842.01 | 0.805688 | 1.183064e-49 | 6.299649e-47 |
| MsG0580029622.01 | MsG0880043842.01 | 0.800794 | 1.219517e-48 | 5.729704e-46 |
| MsG0280010109.01 | MsG0880043842.01 | 0.806230 | 9.098538e-50 | 4.912656e-47 |
| MsG0780036773.01 | MsG0880043842.01 | 0.805286 | 1.436141e-49 | 7.568353e-47 |
| MsG0780039443.01 | MsG0880043842.01 | 0.800971 | 1.122119e-48 | 5.296304e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043842.01.T01 | MTR_8g042170 | 83.582 | 469 | 67 | 4 | 1 | 468 | 1 | 460 | 0.0 | 803 |
| MsG0880043842.01.T01 | MTR_8g037923 | 75.480 | 469 | 113 | 2 | 1 | 468 | 6 | 473 | 0.0 | 731 |
| MsG0880043842.01.T01 | MTR_8g037920 | 71.226 | 424 | 104 | 2 | 46 | 468 | 6 | 412 | 0.0 | 613 |
| MsG0880043842.01.T01 | MTR_8g042400 | 81.267 | 363 | 52 | 2 | 106 | 468 | 1 | 347 | 0.0 | 601 |
| MsG0880043842.01.T01 | MTR_8g042070 | 87.640 | 267 | 31 | 1 | 201 | 467 | 1 | 265 | 2.61e-160 | 454 |
| MsG0880043842.01.T01 | MTR_8g042080 | 87.647 | 170 | 20 | 1 | 1 | 169 | 1 | 170 | 2.81e-105 | 310 |
| MsG0880043842.01.T01 | MTR_7g060310 | 36.986 | 511 | 232 | 12 | 10 | 467 | 16 | 489 | 1.23e-97 | 302 |
| MsG0880043842.01.T01 | MTR_7g059540 | 37.000 | 500 | 250 | 13 | 6 | 467 | 5 | 477 | 1.60e-92 | 289 |
| MsG0880043842.01.T01 | MTR_7g060320 | 37.924 | 501 | 236 | 14 | 1 | 467 | 7 | 466 | 1.88e-92 | 288 |
| MsG0880043842.01.T01 | MTR_7g059540 | 36.800 | 500 | 241 | 14 | 6 | 467 | 6 | 468 | 1.06e-91 | 286 |
| MsG0880043842.01.T01 | MTR_7g060260 | 38.526 | 475 | 224 | 12 | 8 | 467 | 7 | 428 | 5.33e-87 | 273 |
| MsG0880043842.01.T01 | MTR_7g446270 | 37.917 | 480 | 228 | 12 | 24 | 461 | 29 | 480 | 1.26e-85 | 271 |
| MsG0880043842.01.T01 | MTR_7g060115 | 37.552 | 482 | 206 | 14 | 24 | 467 | 25 | 449 | 1.56e-85 | 270 |
| MsG0880043842.01.T01 | MTR_7g060330 | 36.948 | 498 | 222 | 12 | 24 | 467 | 30 | 489 | 1.58e-85 | 271 |
| MsG0880043842.01.T01 | MTR_7g060840 | 35.656 | 488 | 233 | 13 | 1 | 467 | 7 | 434 | 1.62e-85 | 270 |
| MsG0880043842.01.T01 | MTR_0182s0060 | 35.283 | 530 | 255 | 14 | 8 | 467 | 7 | 518 | 2.01e-85 | 272 |
| MsG0880043842.01.T01 | MTR_7g060020 | 36.774 | 465 | 209 | 12 | 24 | 467 | 29 | 429 | 1.72e-84 | 266 |
| MsG0880043842.01.T01 | MTR_7g059810 | 37.393 | 468 | 222 | 14 | 24 | 467 | 26 | 446 | 8.04e-84 | 265 |
| MsG0880043842.01.T01 | MTR_7g060370 | 36.853 | 502 | 246 | 13 | 1 | 467 | 7 | 472 | 2.68e-82 | 262 |
| MsG0880043842.01.T01 | MTR_7g060220 | 34.255 | 470 | 227 | 13 | 24 | 467 | 25 | 438 | 1.67e-72 | 236 |
| MsG0880043842.01.T01 | MTR_7g060160 | 34.599 | 474 | 218 | 12 | 8 | 467 | 6 | 401 | 4.40e-72 | 234 |
| MsG0880043842.01.T01 | MTR_7g059510 | 32.347 | 473 | 209 | 10 | 24 | 467 | 29 | 419 | 7.82e-66 | 218 |
| MsG0880043842.01.T01 | MTR_7g059525 | 42.164 | 268 | 121 | 5 | 211 | 467 | 41 | 285 | 1.45e-60 | 200 |
| MsG0880043842.01.T01 | MTR_8g042090 | 85.047 | 107 | 16 | 0 | 321 | 427 | 2 | 108 | 6.23e-60 | 192 |
| MsG0880043842.01.T01 | MTR_7g059480 | 49.744 | 195 | 83 | 2 | 273 | 467 | 596 | 775 | 6.50e-55 | 196 |
| MsG0880043842.01.T01 | MTR_7g059480 | 41.237 | 194 | 89 | 7 | 1 | 182 | 6 | 186 | 2.01e-33 | 134 |
| MsG0880043842.01.T01 | MTR_7g060080 | 45.641 | 195 | 91 | 2 | 273 | 467 | 14 | 193 | 8.79e-54 | 179 |
| MsG0880043842.01.T01 | MTR_7g059520 | 40.816 | 196 | 91 | 5 | 273 | 467 | 3 | 174 | 8.67e-42 | 147 |
| MsG0880043842.01.T01 | MTR_7g059515 | 44.099 | 161 | 71 | 4 | 24 | 175 | 25 | 175 | 1.28e-35 | 131 |
| MsG0880043842.01.T01 | MTR_5g086340 | 27.115 | 461 | 232 | 15 | 36 | 465 | 45 | 432 | 2.15e-32 | 129 |
| MsG0880043842.01.T01 | MTR_8g042830 | 26.754 | 456 | 233 | 16 | 26 | 465 | 43 | 413 | 2.96e-31 | 125 |
| MsG0880043842.01.T01 | MTR_5g086260 | 34.404 | 218 | 115 | 6 | 251 | 466 | 118 | 309 | 8.13e-27 | 110 |
| MsG0880043842.01.T01 | MTR_7g060060 | 40.506 | 158 | 76 | 4 | 48 | 196 | 1 | 149 | 8.22e-26 | 105 |
| MsG0880043842.01.T01 | MTR_5g016570 | 35.450 | 189 | 104 | 6 | 277 | 465 | 356 | 526 | 7.92e-25 | 107 |
| MsG0880043842.01.T01 | MTR_2g036320 | 25.268 | 467 | 260 | 16 | 16 | 461 | 62 | 460 | 1.94e-22 | 100 |
| MsG0880043842.01.T01 | MTR_5g087580 | 31.414 | 191 | 112 | 5 | 275 | 465 | 43 | 214 | 3.19e-22 | 95.1 |
| MsG0880043842.01.T01 | MTR_2g041180 | 26.190 | 462 | 263 | 19 | 16 | 461 | 45 | 444 | 4.02e-21 | 96.3 |
| MsG0880043842.01.T01 | MTR_1g048070 | 35.583 | 163 | 70 | 5 | 19 | 174 | 23 | 157 | 9.29e-19 | 85.9 |
| MsG0880043842.01.T01 | MTR_8g005325 | 26.174 | 447 | 239 | 17 | 44 | 457 | 74 | 462 | 2.26e-18 | 87.8 |
| MsG0880043842.01.T01 | MTR_7g059475 | 27.481 | 262 | 129 | 8 | 69 | 292 | 1 | 239 | 9.11e-17 | 80.5 |
| MsG0880043842.01.T01 | MTR_2g036310 | 28.235 | 170 | 106 | 4 | 299 | 465 | 2 | 158 | 5.26e-16 | 75.9 |
| MsG0880043842.01.T01 | MTR_8g005235 | 23.394 | 436 | 264 | 14 | 44 | 457 | 45 | 432 | 1.29e-15 | 79.3 |
| MsG0880043842.01.T01 | MTR_6g011050 | 23.356 | 441 | 271 | 15 | 26 | 461 | 26 | 404 | 6.53e-14 | 73.9 |
| MsG0880043842.01.T01 | MTR_6g010810 | 24.944 | 445 | 260 | 20 | 26 | 461 | 24 | 403 | 1.20e-13 | 72.8 |
| MsG0880043842.01.T01 | MTR_7g060200 | 44.186 | 86 | 37 | 3 | 94 | 170 | 19 | 102 | 1.54e-13 | 69.7 |
| MsG0880043842.01.T01 | MTR_6g010850 | 29.747 | 158 | 95 | 4 | 305 | 460 | 200 | 343 | 6.90e-12 | 67.4 |
| MsG0880043842.01.T01 | MTR_7g011980 | 26.042 | 192 | 121 | 5 | 276 | 465 | 169 | 341 | 2.52e-11 | 65.5 |
| MsG0880043842.01.T01 | MTR_4g009590 | 26.180 | 233 | 137 | 8 | 239 | 465 | 347 | 550 | 5.01e-11 | 65.5 |
| MsG0880043842.01.T01 | MTR_4g015410 | 23.499 | 383 | 200 | 15 | 113 | 464 | 49 | 369 | 9.49e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043842.01.T01 | AT3G50120 | 27.447 | 470 | 260 | 18 | 26 | 465 | 105 | 523 | 8.54e-40 | 150 |
| MsG0880043842.01.T01 | AT3G50170 | 27.731 | 476 | 249 | 19 | 26 | 465 | 115 | 531 | 7.39e-36 | 139 |
| MsG0880043842.01.T01 | AT3G50150 | 25.446 | 448 | 288 | 15 | 26 | 465 | 92 | 501 | 1.33e-33 | 133 |
| MsG0880043842.01.T01 | AT3G50160 | 26.770 | 452 | 255 | 18 | 26 | 465 | 106 | 493 | 5.11e-33 | 131 |
| MsG0880043842.01.T01 | AT3G50130 | 28.358 | 469 | 258 | 19 | 26 | 467 | 141 | 558 | 1.44e-32 | 130 |
| MsG0880043842.01.T01 | AT3G50180 | 26.222 | 450 | 271 | 14 | 26 | 464 | 180 | 579 | 7.48e-32 | 129 |
| MsG0880043842.01.T01 | AT3G50140 | 25.536 | 466 | 276 | 19 | 26 | 467 | 115 | 533 | 1.98e-31 | 127 |
| MsG0880043842.01.T01 | AT5G22550 | 26.042 | 480 | 270 | 22 | 26 | 464 | 46 | 481 | 2.63e-28 | 117 |
| MsG0880043842.01.T01 | AT3G50190 | 23.478 | 460 | 260 | 18 | 26 | 465 | 98 | 485 | 5.25e-25 | 108 |
| MsG0880043842.01.T01 | AT2G36430 | 25.720 | 486 | 272 | 16 | 3 | 465 | 23 | 442 | 3.16e-23 | 102 |
| MsG0880043842.01.T01 | AT5G22550 | 24.313 | 473 | 238 | 20 | 26 | 464 | 46 | 432 | 2.49e-21 | 96.7 |
| MsG0880043842.01.T01 | AT3G44710 | 23.387 | 496 | 315 | 18 | 4 | 464 | 30 | 495 | 1.48e-20 | 94.7 |
| MsG0880043842.01.T01 | AT3G47200 | 23.427 | 461 | 279 | 16 | 26 | 465 | 48 | 455 | 1.65e-20 | 94.4 |
| MsG0880043842.01.T01 | AT3G47200 | 23.427 | 461 | 279 | 16 | 26 | 465 | 48 | 455 | 1.65e-20 | 94.4 |
| MsG0880043842.01.T01 | AT2G28580 | 25.764 | 458 | 252 | 17 | 26 | 465 | 111 | 498 | 2.99e-19 | 90.9 |
| MsG0880043842.01.T01 | AT2G28580 | 25.764 | 458 | 252 | 17 | 26 | 465 | 125 | 512 | 3.95e-19 | 90.5 |
| MsG0880043842.01.T01 | AT5G22540 | 24.183 | 459 | 266 | 18 | 26 | 464 | 33 | 429 | 2.23e-18 | 87.8 |
| MsG0880043842.01.T01 | AT2G28580 | 24.733 | 469 | 260 | 15 | 26 | 465 | 43 | 447 | 8.28e-18 | 86.3 |
| MsG0880043842.01.T01 | AT2G28580 | 24.733 | 469 | 260 | 15 | 26 | 465 | 125 | 529 | 9.69e-18 | 86.3 |
| MsG0880043842.01.T01 | AT2G28580 | 24.733 | 469 | 260 | 15 | 26 | 465 | 111 | 515 | 1.08e-17 | 86.3 |
| MsG0880043842.01.T01 | AT2G44930 | 24.094 | 469 | 268 | 14 | 26 | 465 | 86 | 495 | 3.09e-16 | 81.6 |
| MsG0880043842.01.T01 | AT5G22560 | 23.780 | 492 | 290 | 18 | 26 | 464 | 49 | 508 | 6.07e-16 | 80.5 |
| MsG0880043842.01.T01 | AT4G31980 | 22.343 | 461 | 259 | 16 | 26 | 465 | 295 | 677 | 7.43e-16 | 80.9 |
| MsG0880043842.01.T01 | AT3G47250 | 21.645 | 462 | 288 | 18 | 26 | 465 | 63 | 472 | 4.12e-15 | 77.8 |
| MsG0880043842.01.T01 | AT3G47250 | 21.645 | 462 | 288 | 18 | 26 | 465 | 63 | 472 | 4.12e-15 | 77.8 |
| MsG0880043842.01.T01 | AT3G47250 | 21.645 | 462 | 288 | 18 | 26 | 465 | 63 | 472 | 4.12e-15 | 77.8 |
| MsG0880043842.01.T01 | AT1G65985 | 24.176 | 455 | 268 | 20 | 24 | 443 | 14 | 426 | 3.85e-11 | 65.5 |
| MsG0880043842.01.T01 | AT1G65985 | 24.176 | 455 | 268 | 20 | 24 | 443 | 14 | 426 | 3.85e-11 | 65.5 |
Find 73 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTTCTTTGCTACACTTTA+TGG | 0.073408 | 8:+31470442 | MsG0880043842.01.T01:CDS |
| AATTAACAATTGTGATAATT+TGG | 0.081397 | 8:+31470616 | MsG0880043842.01.T01:CDS |
| ATTGGAACCCTTTGGATCTT+AGG | 0.174609 | 8:-31470128 | None:intergenic |
| CGAAGAAACAGACATTAATT+TGG | 0.188689 | 8:+31470586 | MsG0880043842.01.T01:CDS |
| GCAAAGCTCTTTAATGATTT+AGG | 0.189385 | 8:+31471199 | MsG0880043842.01.T01:CDS |
| TTCAATGTTGGAAACCTTAT+TGG | 0.207962 | 8:-31470732 | None:intergenic |
| TGCCATTAATGCGGCTAAAA+AGG | 0.232548 | 8:-31471395 | None:intergenic |
| TTGCTCGTTCTTTACCTTCA+TGG | 0.271366 | 8:+31471090 | MsG0880043842.01.T01:CDS |
| GTTGGTCCAACTTAAGGTTT+AGG | 0.287492 | 8:-31470493 | None:intergenic |
| AGGAAGAAATTAATTTGAAT+TGG | 0.289187 | 8:+31470843 | MsG0880043842.01.T01:CDS |
| ATTAACAATTGTGATAATTT+GGG | 0.297105 | 8:+31470617 | MsG0880043842.01.T01:CDS |
| TCAACATTTGTTGCTAACTA+TGG | 0.298948 | 8:+31470272 | MsG0880043842.01.T01:CDS |
| ACATTTCGTTCAAGTCTAAA+TGG | 0.314952 | 8:+31470954 | MsG0880043842.01.T01:CDS |
| AAATTAATTTGAATTGGAAT+TGG | 0.317522 | 8:+31470849 | MsG0880043842.01.T01:CDS |
| CTTTACCTTCATGGATTCAT+TGG | 0.318220 | 8:+31471099 | MsG0880043842.01.T01:CDS |
| TTGGTCCAACTTAAGGTTTA+GGG | 0.332201 | 8:-31470492 | None:intergenic |
| AACTTAAGGTTTAGGGCTTC+CGG | 0.343209 | 8:-31470485 | None:intergenic |
| GCATCAGTTGGTCCAACTTA+AGG | 0.352988 | 8:-31470499 | None:intergenic |
| GGACCAAATGATATGATCTT+TGG | 0.358041 | 8:-31470191 | None:intergenic |
| GATTTATGGCAAACCAGGTT+TGG | 0.364456 | 8:-31471435 | None:intergenic |
| GAGAGCATTACAAGCTTCTA+TGG | 0.369567 | 8:+31470246 | MsG0880043842.01.T01:CDS |
| TCAATGTTGGAAACCTTATT+GGG | 0.373913 | 8:-31470731 | None:intergenic |
| GTGAATATATTTCTTGCTAT+AGG | 0.374230 | 8:-31471275 | None:intergenic |
| ATGGAGAGATACTTTATTGT+TGG | 0.375548 | 8:+31470526 | MsG0880043842.01.T01:CDS |
| ACTTAAGGTTTAGGGCTTCC+GGG | 0.381935 | 8:-31470484 | None:intergenic |
| GCTTGGAAGCGACGAAGAAT+TGG | 0.396278 | 8:+31471177 | MsG0880043842.01.T01:CDS |
| AGGGTACTTGTCACCCAATA+AGG | 0.397724 | 8:+31470718 | MsG0880043842.01.T01:CDS |
| TTCAAGTCTAAATGGTTTAG+TGG | 0.405983 | 8:+31470962 | MsG0880043842.01.T01:CDS |
| AACTATGGCGAAATTGATAA+AGG | 0.408429 | 8:+31470287 | MsG0880043842.01.T01:CDS |
| TGGAATGGTAATGGTCCAAT+TGG | 0.413103 | 8:+31470655 | MsG0880043842.01.T01:CDS |
| ATAAGACAGATAACTTGAAC+TGG | 0.424070 | 8:+31470927 | MsG0880043842.01.T01:CDS |
| TTCGCCATGTTCTTCAATGT+TGG | 0.433622 | 8:-31470744 | None:intergenic |
| TCTTGCTTGGATATTGTTTG+TGG | 0.441123 | 8:+31470415 | MsG0880043842.01.T01:CDS |
| TCATACTTGCAATGAACATC+TGG | 0.445620 | 8:-31471064 | None:intergenic |
| CATTGAAGAACATGGCGAAA+TGG | 0.446634 | 8:+31470748 | MsG0880043842.01.T01:CDS |
| GGTTTAGGGCTTCCGGGCAT+TGG | 0.455070 | 8:-31470478 | None:intergenic |
| GGTGTATTTCAAAACTTGCT+TGG | 0.456888 | 8:+31471160 | MsG0880043842.01.T01:CDS |
| AGAATCTCAAGCATTTCTCT+TGG | 0.458501 | 8:-31470560 | None:intergenic |
| AGGGCTTAGGATCTTCCAAT+TGG | 0.458983 | 8:-31470670 | None:intergenic |
| ACGCCAAAGATCATATCATT+TGG | 0.461742 | 8:+31470188 | MsG0880043842.01.T01:CDS |
| GTAAAAGAACTTAGATCAGC+TGG | 0.462024 | 8:+31471139 | MsG0880043842.01.T01:CDS |
| TGGACCAACTGATGCACATA+TGG | 0.485178 | 8:+31470507 | MsG0880043842.01.T01:CDS |
| TTCACTGAGGATGCAATTGA+AGG | 0.489786 | 8:+31470377 | MsG0880043842.01.T01:CDS |
| GGAAGCCCTAAACCTTAAGT+TGG | 0.491856 | 8:+31470487 | MsG0880043842.01.T01:CDS |
| GGAATACTTATAAGAGCATA+CGG | 0.496169 | 8:+31470870 | MsG0880043842.01.T01:CDS |
| TCGAGTATATGAAAGGGCTT+AGG | 0.498991 | 8:-31470683 | None:intergenic |
| GCTTGGATATTGTTTGTGGA+TGG | 0.502147 | 8:+31470419 | MsG0880043842.01.T01:CDS |
| AGCAAAATCGAGTATATGAA+AGG | 0.504765 | 8:-31470690 | None:intergenic |
| TTGGGTGAATGCAAACGACT+TGG | 0.512870 | 8:+31470635 | MsG0880043842.01.T01:CDS |
| AATGGTTTAGTGGAGAACTA+AGG | 0.515095 | 8:+31470972 | MsG0880043842.01.T01:CDS |
| ACAGTGATAATGATCTTGCT+TGG | 0.524232 | 8:+31470402 | MsG0880043842.01.T01:CDS |
| TTCTTACTTTCATCCAAACC+TGG | 0.527818 | 8:+31471422 | MsG0880043842.01.T01:CDS |
| CAAACGACTTGGAATGGTAA+TGG | 0.528526 | 8:+31470646 | MsG0880043842.01.T01:CDS |
| CTCTCCATATGTGCATCAGT+TGG | 0.531103 | 8:-31470511 | None:intergenic |
| TTTAGGATTTATGGCAAACC+AGG | 0.540085 | 8:-31471440 | None:intergenic |
| TTTGATTCCTAAGATCCAAA+GGG | 0.551215 | 8:+31470121 | MsG0880043842.01.T01:CDS |
| GCAAAATCGAGTATATGAAA+GGG | 0.560907 | 8:-31470689 | None:intergenic |
| ATACGGGATCTAAAGACAGC+AGG | 0.564830 | 8:+31470887 | MsG0880043842.01.T01:CDS |
| AGTTATCTACTATGGATGAA+AGG | 0.569581 | 8:+31470048 | None:intergenic |
| AAATAAATGGAAGACATGGT+TGG | 0.569744 | 8:+31471327 | MsG0880043842.01.T01:CDS |
| CACTCATTTCAATACACCAT+GGG | 0.583087 | 8:+31471363 | MsG0880043842.01.T01:CDS |
| GTCAACCAATGAATCCATGA+AGG | 0.593693 | 8:-31471104 | None:intergenic |
| AAATGTTGATGACCAATGCC+CGG | 0.597421 | 8:+31470466 | MsG0880043842.01.T01:CDS |
| GAAATGGAAACAAAATCTGA+AGG | 0.617064 | 8:+31470764 | MsG0880043842.01.T01:CDS |
| TGAATGCAAACGACTTGGAA+TGG | 0.638011 | 8:+31470640 | MsG0880043842.01.T01:CDS |
| AAGGCTTTCGCAACTCAAAG+AGG | 0.638415 | 8:+31470067 | MsG0880043842.01.T01:CDS |
| GTTTCCAACATTGAAGAACA+TGG | 0.638979 | 8:+31470740 | MsG0880043842.01.T01:CDS |
| ACACTCATTTCAATACACCA+TGG | 0.648316 | 8:+31471362 | MsG0880043842.01.T01:CDS |
| TTCATTGGTTGACAATGCAG+AGG | 0.655855 | 8:+31471114 | MsG0880043842.01.T01:CDS |
| AAGAACTAATGCCATTAATG+CGG | 0.659409 | 8:-31471404 | None:intergenic |
| AAAGACAGCAGGAATTCAAG+TGG | 0.660546 | 8:+31470898 | MsG0880043842.01.T01:CDS |
| GAATACTTATAAGAGCATAC+GGG | 0.665130 | 8:+31470871 | MsG0880043842.01.T01:CDS |
| TGTGAAGAATATGTTCACTG+AGG | 0.803057 | 8:+31470364 | MsG0880043842.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAACAATTGTGATAATT+TGG | + | Chr8:31470616-31470635 | MsG0880043842.01.T01:CDS | 15.0% |
| !! | ATTAACAATTGTGATAATTT+GGG | + | Chr8:31470617-31470636 | MsG0880043842.01.T01:CDS | 15.0% |
| !! | ATTAAGTTTCGAAAAAAATA+AGG | - | Chr8:31471028-31471047 | None:intergenic | 15.0% |
| !!! | AAAAGCATTACAAAAATAAA+TGG | + | Chr8:31471314-31471333 | MsG0880043842.01.T01:CDS | 15.0% |
| !!! | AAATTAATTTGAATTGGAAT+TGG | + | Chr8:31470849-31470868 | MsG0880043842.01.T01:CDS | 15.0% |
| !!! | AGGAAGAAATTAATTTGAAT+TGG | + | Chr8:31470843-31470862 | MsG0880043842.01.T01:CDS | 20.0% |
| ! | AATGCTAAAAATCTGAAAGA+AGG | + | Chr8:31470224-31470243 | MsG0880043842.01.T01:CDS | 25.0% |
| ! | ACAAAAATAAATGGAAGACA+TGG | + | Chr8:31471323-31471342 | MsG0880043842.01.T01:CDS | 25.0% |
| ! | ACGTCATTGAACAAAAAAAT+AGG | - | Chr8:31471001-31471020 | None:intergenic | 25.0% |
| !! | GTGAATATATTTCTTGCTAT+AGG | - | Chr8:31471278-31471297 | None:intergenic | 25.0% |
| !!! | TTTTTAGCATTGTGATGAAT+CGG | - | Chr8:31470215-31470234 | None:intergenic | 25.0% |
| AAATAAATGGAAGACATGGT+TGG | + | Chr8:31471327-31471346 | MsG0880043842.01.T01:CDS | 30.0% | |
| AACTATGGCGAAATTGATAA+AGG | + | Chr8:31470287-31470306 | MsG0880043842.01.T01:CDS | 30.0% | |
| AAGAACTAATGCCATTAATG+CGG | - | Chr8:31471407-31471426 | None:intergenic | 30.0% | |
| ACATTTCGTTCAAGTCTAAA+TGG | + | Chr8:31470954-31470973 | MsG0880043842.01.T01:CDS | 30.0% | |
| AGCAAAATCGAGTATATGAA+AGG | - | Chr8:31470693-31470712 | None:intergenic | 30.0% | |
| ATAAGACAGATAACTTGAAC+TGG | + | Chr8:31470927-31470946 | MsG0880043842.01.T01:CDS | 30.0% | |
| ATGGAGAGATACTTTATTGT+TGG | + | Chr8:31470526-31470545 | MsG0880043842.01.T01:CDS | 30.0% | |
| ATGTTCTTTGCTACACTTTA+TGG | + | Chr8:31470442-31470461 | MsG0880043842.01.T01:CDS | 30.0% | |
| CGAAGAAACAGACATTAATT+TGG | + | Chr8:31470586-31470605 | MsG0880043842.01.T01:CDS | 30.0% | |
| GAAATGGAAACAAAATCTGA+AGG | + | Chr8:31470764-31470783 | MsG0880043842.01.T01:CDS | 30.0% | |
| GCAAAATCGAGTATATGAAA+GGG | - | Chr8:31470692-31470711 | None:intergenic | 30.0% | |
| GCAAAGCTCTTTAATGATTT+AGG | + | Chr8:31471199-31471218 | MsG0880043842.01.T01:CDS | 30.0% | |
| TCAATGTTGGAAACCTTATT+GGG | - | Chr8:31470734-31470753 | None:intergenic | 30.0% | |
| TTCAAGTCTAAATGGTTTAG+TGG | + | Chr8:31470962-31470981 | MsG0880043842.01.T01:CDS | 30.0% | |
| TTCAATGTTGGAAACCTTAT+TGG | - | Chr8:31470735-31470754 | None:intergenic | 30.0% | |
| TTTGATTCCTAAGATCCAAA+GGG | + | Chr8:31470121-31470140 | MsG0880043842.01.T01:CDS | 30.0% | |
| ! | ATGTGACAACACATTTTTGT+TGG | - | Chr8:31471235-31471254 | None:intergenic | 30.0% |
| ! | GAATACTTATAAGAGCATAC+GGG | + | Chr8:31470871-31470890 | MsG0880043842.01.T01:CDS | 30.0% |
| ! | GGAATACTTATAAGAGCATA+CGG | + | Chr8:31470870-31470889 | MsG0880043842.01.T01:CDS | 30.0% |
| ! | TCAACATTTGTTGCTAACTA+TGG | + | Chr8:31470272-31470291 | MsG0880043842.01.T01:CDS | 30.0% |
| !! | TTTTGATTCCTAAGATCCAA+AGG | + | Chr8:31470120-31470139 | MsG0880043842.01.T01:CDS | 30.0% |
| AATGGTTTAGTGGAGAACTA+AGG | + | Chr8:31470972-31470991 | MsG0880043842.01.T01:CDS | 35.0% | |
| ACACTCATTTCAATACACCA+TGG | + | Chr8:31471362-31471381 | MsG0880043842.01.T01:CDS | 35.0% | |
| ACGCCAAAGATCATATCATT+TGG | + | Chr8:31470188-31470207 | MsG0880043842.01.T01:CDS | 35.0% | |
| AGAATCTCAAGCATTTCTCT+TGG | - | Chr8:31470563-31470582 | None:intergenic | 35.0% | |
| AGACCAAAAAGATAGTGATG+AGG | + | Chr8:31470823-31470842 | MsG0880043842.01.T01:CDS | 35.0% | |
| CACTCATTTCAATACACCAT+GGG | + | Chr8:31471363-31471382 | MsG0880043842.01.T01:CDS | 35.0% | |
| CTTTACCTTCATGGATTCAT+TGG | + | Chr8:31471099-31471118 | MsG0880043842.01.T01:CDS | 35.0% | |
| GGACCAAATGATATGATCTT+TGG | - | Chr8:31470194-31470213 | None:intergenic | 35.0% | |
| GTAAAAGAACTTAGATCAGC+TGG | + | Chr8:31471139-31471158 | MsG0880043842.01.T01:CDS | 35.0% | |
| GTTTCCAACATTGAAGAACA+TGG | + | Chr8:31470740-31470759 | MsG0880043842.01.T01:CDS | 35.0% | |
| TCATACTTGCAATGAACATC+TGG | - | Chr8:31471067-31471086 | None:intergenic | 35.0% | |
| TCTTGCTTGGATATTGTTTG+TGG | + | Chr8:31470415-31470434 | MsG0880043842.01.T01:CDS | 35.0% | |
| TGTGAAGAATATGTTCACTG+AGG | + | Chr8:31470364-31470383 | MsG0880043842.01.T01:CDS | 35.0% | |
| TTCTTACTTTCATCCAAACC+TGG | + | Chr8:31471422-31471441 | MsG0880043842.01.T01:CDS | 35.0% | |
| TTGGTCCAACTTAAGGTTTA+GGG | - | Chr8:31470495-31470514 | None:intergenic | 35.0% | |
| ! | AACTCAAAGAGGCACAAAAA+AGG | + | Chr8:31470078-31470097 | MsG0880043842.01.T01:CDS | 35.0% |
| ! | ACAGTGATAATGATCTTGCT+TGG | + | Chr8:31470402-31470421 | MsG0880043842.01.T01:CDS | 35.0% |
| ! | CTTCCTCATCACTATCTTTT+TGG | - | Chr8:31470829-31470848 | None:intergenic | 35.0% |
| ! | GGTGTATTTCAAAACTTGCT+TGG | + | Chr8:31471160-31471179 | MsG0880043842.01.T01:CDS | 35.0% |
| ! | TCATTTTGCCGCAAAAACAT+TGG | - | Chr8:31470149-31470168 | None:intergenic | 35.0% |
| ! | TTTAGGATTTATGGCAAACC+AGG | - | Chr8:31471443-31471462 | None:intergenic | 35.0% |
| !! | AAAGGGTTCCAATGTTTTTG+CGG | + | Chr8:31470138-31470157 | MsG0880043842.01.T01:CDS | 35.0% |
| AAAAAGGCAATCATAGCCCA+TGG | - | Chr8:31471382-31471401 | None:intergenic | 40.0% | |
| AAAGACAGCAGGAATTCAAG+TGG | + | Chr8:31470898-31470917 | MsG0880043842.01.T01:CDS | 40.0% | |
| ATTGGAACCCTTTGGATCTT+AGG | - | Chr8:31470131-31470150 | None:intergenic | 40.0% | |
| CATTGAAGAACATGGCGAAA+TGG | + | Chr8:31470748-31470767 | MsG0880043842.01.T01:CDS | 40.0% | |
| GATTTATGGCAAACCAGGTT+TGG | - | Chr8:31471438-31471457 | None:intergenic | 40.0% | |
| GCAAAAACATTGGAACCCTT+TGG | - | Chr8:31470139-31470158 | None:intergenic | 40.0% | |
| GCTTGGATATTGTTTGTGGA+TGG | + | Chr8:31470419-31470438 | MsG0880043842.01.T01:CDS | 40.0% | |
| GTCAACCAATGAATCCATGA+AGG | - | Chr8:31471107-31471126 | None:intergenic | 40.0% | |
| GTTGGTCCAACTTAAGGTTT+AGG | - | Chr8:31470496-31470515 | None:intergenic | 40.0% | |
| TCGAGTATATGAAAGGGCTT+AGG | - | Chr8:31470686-31470705 | None:intergenic | 40.0% | |
| TGCCATTAATGCGGCTAAAA+AGG | - | Chr8:31471398-31471417 | None:intergenic | 40.0% | |
| TTCACTGAGGATGCAATTGA+AGG | + | Chr8:31470377-31470396 | MsG0880043842.01.T01:CDS | 40.0% | |
| TTCGCCATGTTCTTCAATGT+TGG | - | Chr8:31470747-31470766 | None:intergenic | 40.0% | |
| TTGCTCGTTCTTTACCTTCA+TGG | + | Chr8:31471090-31471109 | MsG0880043842.01.T01:CDS | 40.0% | |
| ! | AACTTAAGGTTTAGGGCTTC+CGG | - | Chr8:31470488-31470507 | None:intergenic | 40.0% |
| ! | ATACTCGATTTTGCTCGTTC+AGG | + | Chr8:31470698-31470717 | MsG0880043842.01.T01:CDS | 40.0% |
| ! | CAAACGACTTGGAATGGTAA+TGG | + | Chr8:31470646-31470665 | MsG0880043842.01.T01:CDS | 40.0% |
| ! | TACTCGATTTTGCTCGTTCA+GGG | + | Chr8:31470699-31470718 | MsG0880043842.01.T01:CDS | 40.0% |
| ! | TGAATGCAAACGACTTGGAA+TGG | + | Chr8:31470640-31470659 | MsG0880043842.01.T01:CDS | 40.0% |
| ! | TTCATTGGTTGACAATGCAG+AGG | + | Chr8:31471114-31471133 | MsG0880043842.01.T01:CDS | 40.0% |
| !! | AAATGTTGATGACCAATGCC+CGG | + | Chr8:31470466-31470485 | MsG0880043842.01.T01:CDS | 40.0% |
| !! | GAGAGCATTACAAGCTTCTA+TGG | + | Chr8:31470246-31470265 | MsG0880043842.01.T01:CDS | 40.0% |
| !! | TGCCTTTTTAGCCGCATTAA+TGG | + | Chr8:31471393-31471412 | MsG0880043842.01.T01:CDS | 40.0% |
| !! | TGGAATGGTAATGGTCCAAT+TGG | + | Chr8:31470655-31470674 | MsG0880043842.01.T01:CDS | 40.0% |
| AAGGCTTTCGCAACTCAAAG+AGG | + | Chr8:31470067-31470086 | MsG0880043842.01.T01:CDS | 45.0% | |
| AGGGCTTAGGATCTTCCAAT+TGG | - | Chr8:31470673-31470692 | None:intergenic | 45.0% | |
| ATACGGGATCTAAAGACAGC+AGG | + | Chr8:31470887-31470906 | MsG0880043842.01.T01:CDS | 45.0% | |
| CTCTCCATATGTGCATCAGT+TGG | - | Chr8:31470514-31470533 | None:intergenic | 45.0% | |
| GCATCAGTTGGTCCAACTTA+AGG | - | Chr8:31470502-31470521 | None:intergenic | 45.0% | |
| GGAAGCCCTAAACCTTAAGT+TGG | + | Chr8:31470487-31470506 | MsG0880043842.01.T01:CDS | 45.0% | |
| TGGACCAACTGATGCACATA+TGG | + | Chr8:31470507-31470526 | MsG0880043842.01.T01:CDS | 45.0% | |
| TTGGGTGAATGCAAACGACT+TGG | + | Chr8:31470635-31470654 | MsG0880043842.01.T01:CDS | 45.0% | |
| ! | ACTTAAGGTTTAGGGCTTCC+GGG | - | Chr8:31470487-31470506 | None:intergenic | 45.0% |
| ! | AGGGTACTTGTCACCCAATA+AGG | + | Chr8:31470718-31470737 | MsG0880043842.01.T01:CDS | 45.0% |
| GCTTGGAAGCGACGAAGAAT+TGG | + | Chr8:31471177-31471196 | MsG0880043842.01.T01:CDS | 50.0% | |
| ! | GGTTTAGGGCTTCCGGGCAT+TGG | - | Chr8:31470481-31470500 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 31470059 | 31471465 | 31470059 | ID=MsG0880043842.01;Name=MsG0880043842.01 |
| Chr8 | mRNA | 31470059 | 31471465 | 31470059 | ID=MsG0880043842.01.T01;Parent=MsG0880043842.01;Name=MsG0880043842.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|468 |
| Chr8 | exon | 31470059 | 31471465 | 31470059 | ID=MsG0880043842.01.T01:exon:9457;Parent=MsG0880043842.01.T01 |
| Chr8 | CDS | 31470059 | 31471465 | 31470059 | ID=MsG0880043842.01.T01:cds;Parent=MsG0880043842.01.T01 |
| Gene Sequence |
| Protein sequence |
