AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029622.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029622.01.T01 MTR_5g089720 97.489 438 10 1 1 438 1 437 0.0 889
MsG0580029622.01.T01 MTR_5g094340 51.795 195 81 4 32 218 53 242 6.24e-62 206
MsG0580029622.01.T01 MTR_3g055960 48.718 195 94 3 32 222 54 246 4.06e-61 205
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029622.01.T01 AT5G12300 56.204 274 86 6 33 296 19 268 1.57e-87 271
MsG0580029622.01.T01 AT1G50570 40.389 411 189 11 29 438 33 388 1.05e-83 262
MsG0580029622.01.T01 AT1G50570 40.389 411 189 11 29 438 33 388 1.05e-83 262
MsG0580029622.01.T01 AT5G55530 49.746 197 93 3 32 224 48 242 8.28e-62 206
MsG0580029622.01.T01 AT5G55530 49.746 197 93 3 32 224 48 242 8.28e-62 206
MsG0580029622.01.T01 AT5G55530 49.746 197 93 3 32 224 48 242 8.28e-62 206

Find 59 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCAACAATTTGTGAAAT+TGG 0.112542 5:+99603417 None:intergenic
CCGCTATGATCTTGTCTTTC+GGG 0.138670 5:+99602595 None:intergenic
AACTTCTTGTTCTCATGATT+TGG 0.220949 5:+99602562 None:intergenic
TGTGTAGCTCTATTCAAATT+TGG 0.233288 5:+99603744 None:intergenic
ACCGCTATGATCTTGTCTTT+CGG 0.271993 5:+99602594 None:intergenic
CTGTTATGAAATGTGAAATT+TGG 0.279391 5:-99603497 MsG0580029622.01.T01:CDS
AGAAATCGACAATAAGAAAA+AGG 0.318863 5:-99602539 MsG0580029622.01.T01:CDS
AGCATAGAAGTAACAAAGTT+TGG 0.319974 5:-99602766 MsG0580029622.01.T01:CDS
TTCCAACAATTTGTGAAATT+GGG 0.331184 5:+99603418 None:intergenic
TTCTTTGAAGTTGGTGTATC+GGG 0.348339 5:+99602865 None:intergenic
ATTATCTTTAGACACATCTT+TGG 0.351395 5:-99603319 MsG0580029622.01.T01:CDS
TCAATTCTTGAATACTCAAT+TGG 0.352551 5:+99603216 None:intergenic
TTGCTGCCATTTCTTCATCT+TGG 0.384771 5:-99603099 MsG0580029622.01.T01:CDS
TCAAGATTAATATTCCCTAA+TGG 0.389292 5:+99602727 None:intergenic
TCCTGACATAAGTATTGTTA+AGG 0.391070 5:-99603190 MsG0580029622.01.T01:CDS
GGGACAAAGAGTAACAAAAT+TGG 0.398931 5:+99602885 None:intergenic
GGAATTGTGAAGGCTTTCAT+TGG 0.401534 5:+99602999 None:intergenic
ATTTCACAAATTGTTGGAAA+AGG 0.401795 5:-99603414 MsG0580029622.01.T01:CDS
CTTGTAGAAAGAGTTTCATC+AGG 0.408913 5:+99603591 None:intergenic
AATGAAAGCCTTCACAATTC+CGG 0.426166 5:-99602997 MsG0580029622.01.T01:CDS
GTCCCAATTTCACAAATTGT+TGG 0.426474 5:-99603420 MsG0580029622.01.T01:CDS
TCACCTTCTTTCTTTGAAGT+TGG 0.429226 5:+99602856 None:intergenic
TTTCTTTGAAGTTGGTGTAT+CGG 0.431713 5:+99602864 None:intergenic
GAGTTCGCCAGATGAGAATC+AGG 0.437738 5:-99603037 MsG0580029622.01.T01:CDS
GAATCCACCTGATTCTCATC+TGG 0.447663 5:+99603030 None:intergenic
TCCTTAACAATACTTATGTC+AGG 0.450696 5:+99603189 None:intergenic
GTCATCAAAACAACAGCTTT+AGG 0.454918 5:-99603773 MsG0580029622.01.T01:CDS
AAGCCTTCACAATTCCGGTT+TGG 0.455785 5:-99602992 MsG0580029622.01.T01:CDS
GCAACAGTTTACTGAGTCCT+TGG 0.468052 5:-99602650 MsG0580029622.01.T01:CDS
GAAGCACCAAGATGAAGAAA+TGG 0.478364 5:+99603093 None:intergenic
AAAATTCTCAAACAAAGAAA+AGG 0.487388 5:-99602806 MsG0580029622.01.T01:CDS
GAATTGTGAAGGCTTTCATT+GGG 0.490123 5:+99603000 None:intergenic
TGTTAAGGAGAATCAACAAA+TGG 0.492303 5:-99603175 MsG0580029622.01.T01:CDS
TGCTATGCAGAAGCAAATAG+TGG 0.492416 5:-99602692 MsG0580029622.01.T01:CDS
AAAGATATCTGAAGTTATGT+TGG 0.495670 5:-99603241 MsG0580029622.01.T01:CDS
GATAGTAGAGCTTACCAAAC+CGG 0.496758 5:+99602978 None:intergenic
AAAAGGAATGCAAGAAGGAA+AGG 0.505483 5:-99602789 MsG0580029622.01.T01:CDS
CAAAGAAAAGGAATGCAAGA+AGG 0.507838 5:-99602794 MsG0580029622.01.T01:CDS
GAAGATGAAGCAGAGTTCAC+AGG 0.514799 5:-99603711 MsG0580029622.01.T01:CDS
GGCGAAAATGAAGCTTCCTA+TGG 0.518944 5:-99602629 MsG0580029622.01.T01:CDS
TCTTGAATTGTTGTTACTTG+AGG 0.524311 5:+99603125 None:intergenic
TCGGGTTTGTCAACGTCCAT+AGG 0.526209 5:+99602613 None:intergenic
TTTCATCAGGGTTGTAAGTG+AGG 0.531627 5:+99603604 None:intergenic
GTTAATTGAACAGTTCCAGC+TGG 0.532682 5:+99603342 None:intergenic
CTTTCTACAAGAATAATCAA+TGG 0.534660 5:-99603579 MsG0580029622.01.T01:CDS
AAATAGTGGATATGTATATG+AGG 0.538443 5:-99602678 MsG0580029622.01.T01:CDS
ACAAGAATAATCAATGGAGG+TGG 0.563554 5:-99603573 MsG0580029622.01.T01:CDS
TTCATCAGGGTTGTAAGTGA+GGG 0.567857 5:+99603605 None:intergenic
ACACCAACTTCAAAGAAAGA+AGG 0.576636 5:-99602859 MsG0580029622.01.T01:CDS
TTACCAAACCGGAATTGTGA+AGG 0.582333 5:+99602989 None:intergenic
CAACATTCAATGAAAACTTG+AGG 0.590629 5:-99603542 MsG0580029622.01.T01:CDS
GAATATTAATCTTGAAGCTG+AGG 0.593724 5:-99602719 MsG0580029622.01.T01:CDS
AAGATGAAGCAGAGTTCACA+GGG 0.598725 5:-99603710 MsG0580029622.01.T01:CDS
TGTTATGAATGTTTCTAGCA+TGG 0.599484 5:+99603667 None:intergenic
TTGTAGAAAGAGTTTCATCA+GGG 0.607403 5:+99603592 None:intergenic
TAGTAGAGCAAGAATTCACA+TGG 0.626136 5:-99603469 MsG0580029622.01.T01:CDS
TCTACAAGAATAATCAATGG+AGG 0.653951 5:-99603576 MsG0580029622.01.T01:CDS
TTCGCCAGATGAGAATCAGG+TGG 0.666779 5:-99603034 MsG0580029622.01.T01:CDS
CCCGAAAGACAAGATCATAG+CGG 0.770714 5:-99602595 MsG0580029622.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTCTCAAACAAAGAAA+AGG - Chr5:99603484-99603503 MsG0580029622.01.T01:CDS 20.0%
!!! AAGTTTTTAATTCTCCATTA+GGG - Chr5:99603549-99603568 MsG0580029622.01.T01:CDS 20.0%
! AGAAATCGACAATAAGAAAA+AGG - Chr5:99603751-99603770 MsG0580029622.01.T01:CDS 25.0%
! ATTATCTTTAGACACATCTT+TGG - Chr5:99602971-99602990 MsG0580029622.01.T01:CDS 25.0%
! ATTTCACAAATTGTTGGAAA+AGG - Chr5:99602876-99602895 MsG0580029622.01.T01:CDS 25.0%
! CTGTTATGAAATGTGAAATT+TGG - Chr5:99602793-99602812 MsG0580029622.01.T01:CDS 25.0%
! CTTTCTACAAGAATAATCAA+TGG - Chr5:99602711-99602730 MsG0580029622.01.T01:CDS 25.0%
! TCAAGATTAATATTCCCTAA+TGG + Chr5:99603566-99603585 None:intergenic 25.0%
! TCAATTCTTGAATACTCAAT+TGG + Chr5:99603077-99603096 None:intergenic 25.0%
! TTCCAACAATTTGTGAAATT+GGG + Chr5:99602875-99602894 None:intergenic 25.0%
! TTTCCAACAATTTGTGAAAT+TGG + Chr5:99602876-99602895 None:intergenic 25.0%
! TTTGAGAAAAAGAATCATCT+AGG - Chr5:99603371-99603390 MsG0580029622.01.T01:CDS 25.0%
!! AAAGATATCTGAAGTTATGT+TGG - Chr5:99603049-99603068 MsG0580029622.01.T01:CDS 25.0%
!!! AAATAGTGGATATGTATATG+AGG - Chr5:99603612-99603631 MsG0580029622.01.T01:CDS 25.0%
!!! CAAGTTTTTAATTCTCCATT+AGG - Chr5:99603548-99603567 MsG0580029622.01.T01:CDS 25.0%
AACTTCTTGTTCTCATGATT+TGG + Chr5:99603731-99603750 None:intergenic 30.0%
AGCATAGAAGTAACAAAGTT+TGG - Chr5:99603524-99603543 MsG0580029622.01.T01:CDS 30.0%
ATCGACAATAAGAAAAAGGA+TGG - Chr5:99603755-99603774 MsG0580029622.01.T01:CDS 30.0%
CAACATTCAATGAAAACTTG+AGG - Chr5:99602748-99602767 MsG0580029622.01.T01:CDS 30.0%
GAAAAAGATCAGTTGAAGAA+AGG + Chr5:99602923-99602942 None:intergenic 30.0%
GAATATTAATCTTGAAGCTG+AGG - Chr5:99603571-99603590 MsG0580029622.01.T01:CDS 30.0%
TCCTTAACAATACTTATGTC+AGG + Chr5:99603104-99603123 None:intergenic 30.0%
TCTACAAGAATAATCAATGG+AGG - Chr5:99602714-99602733 MsG0580029622.01.T01:CDS 30.0%
TGTTAAGGAGAATCAACAAA+TGG - Chr5:99603115-99603134 MsG0580029622.01.T01:CDS 30.0%
TTGTAGAAAGAGTTTCATCA+GGG + Chr5:99602701-99602720 None:intergenic 30.0%
! ACATGGAAGATCAACTTTTA+GGG - Chr5:99602838-99602857 MsG0580029622.01.T01:CDS 30.0%
! CTCAAGTTTTCATTGAATGT+TGG + Chr5:99602750-99602769 None:intergenic 30.0%
! TCCTGACATAAGTATTGTTA+AGG - Chr5:99603100-99603119 MsG0580029622.01.T01:CDS 30.0%
! TCTTGAATTGTTGTTACTTG+AGG + Chr5:99603168-99603187 None:intergenic 30.0%
! TGTGTAGCTCTATTCAAATT+TGG + Chr5:99602549-99602568 None:intergenic 30.0%
!! TGTTATGAATGTTTCTAGCA+TGG + Chr5:99602626-99602645 None:intergenic 30.0%
!! TTTCTTTGAAGTTGGTGTAT+CGG + Chr5:99603429-99603448 None:intergenic 30.0%
AAAAGGAATGCAAGAAGGAA+AGG - Chr5:99603501-99603520 MsG0580029622.01.T01:CDS 35.0%
AATGAAAGCCTTCACAATTC+CGG - Chr5:99603293-99603312 MsG0580029622.01.T01:CDS 35.0%
ACAAGAATAATCAATGGAGG+TGG - Chr5:99602717-99602736 MsG0580029622.01.T01:CDS 35.0%
ACACCAACTTCAAAGAAAGA+AGG - Chr5:99603431-99603450 MsG0580029622.01.T01:CDS 35.0%
CAAAGAAAAGGAATGCAAGA+AGG - Chr5:99603496-99603515 MsG0580029622.01.T01:CDS 35.0%
CTTGTAGAAAGAGTTTCATC+AGG + Chr5:99602702-99602721 None:intergenic 35.0%
GAATTGTGAAGGCTTTCATT+GGG + Chr5:99603293-99603312 None:intergenic 35.0%
GGGACAAAGAGTAACAAAAT+TGG + Chr5:99603408-99603427 None:intergenic 35.0%
GTCATCAAAACAACAGCTTT+AGG - Chr5:99602517-99602536 MsG0580029622.01.T01:CDS 35.0%
GTCCCAATTTCACAAATTGT+TGG - Chr5:99602870-99602889 MsG0580029622.01.T01:CDS 35.0%
TAGTAGAGCAAGAATTCACA+TGG - Chr5:99602821-99602840 MsG0580029622.01.T01:CDS 35.0%
! CACATGGAAGATCAACTTTT+AGG - Chr5:99602837-99602856 MsG0580029622.01.T01:CDS 35.0%
!! TCACCTTCTTTCTTTGAAGT+TGG + Chr5:99603437-99603456 None:intergenic 35.0%
!! TTCTTTGAAGTTGGTGTATC+GGG + Chr5:99603428-99603447 None:intergenic 35.0%
!!! TCAACTTTTAGGGTTTGCTT+TGG - Chr5:99602848-99602867 MsG0580029622.01.T01:CDS 35.0%
AAGATGAAGCAGAGTTCACA+GGG - Chr5:99602580-99602599 MsG0580029622.01.T01:CDS 40.0%
ACCGCTATGATCTTGTCTTT+CGG + Chr5:99603699-99603718 None:intergenic 40.0%
GATAGTAGAGCTTACCAAAC+CGG + Chr5:99603315-99603334 None:intergenic 40.0%
GGAATTGTGAAGGCTTTCAT+TGG + Chr5:99603294-99603313 None:intergenic 40.0%
GTTAATTGAACAGTTCCAGC+TGG + Chr5:99602951-99602970 None:intergenic 40.0%
TGCTATGCAGAAGCAAATAG+TGG - Chr5:99603598-99603617 MsG0580029622.01.T01:CDS 40.0%
TTACCAAACCGGAATTGTGA+AGG + Chr5:99603304-99603323 None:intergenic 40.0%
TTCATCAGGGTTGTAAGTGA+GGG + Chr5:99602688-99602707 None:intergenic 40.0%
TTTCATCAGGGTTGTAAGTG+AGG + Chr5:99602689-99602708 None:intergenic 40.0%
! CTTTTTCTCAAACGAGTCTG+CGG + Chr5:99603363-99603382 None:intergenic 40.0%
! GAAGCACCAAGATGAAGAAA+TGG + Chr5:99603200-99603219 None:intergenic 40.0%
! TTGCTGCCATTTCTTCATCT+TGG - Chr5:99603191-99603210 MsG0580029622.01.T01:CDS 40.0%
!! GATCTTTTTCATTCTCCAGC+TGG - Chr5:99602933-99602952 MsG0580029622.01.T01:CDS 40.0%
AAGCCTTCACAATTCCGGTT+TGG - Chr5:99603298-99603317 MsG0580029622.01.T01:CDS 45.0%
CCCGAAAGACAAGATCATAG+CGG - Chr5:99603695-99603714 MsG0580029622.01.T01:CDS 45.0%
CCGCTATGATCTTGTCTTTC+GGG + Chr5:99603698-99603717 None:intergenic 45.0%
GAAGATGAAGCAGAGTTCAC+AGG - Chr5:99602579-99602598 MsG0580029622.01.T01:CDS 45.0%
GAATCCACCTGATTCTCATC+TGG + Chr5:99603263-99603282 None:intergenic 45.0%
GCAACAGTTTACTGAGTCCT+TGG - Chr5:99603640-99603659 MsG0580029622.01.T01:CDS 45.0%
GGCGAAAATGAAGCTTCCTA+TGG - Chr5:99603661-99603680 MsG0580029622.01.T01:CDS 45.0%
! AGGAAGCTTCATTTTCGCCA+AGG + Chr5:99603660-99603679 None:intergenic 45.0%
! GATGGTTCGCGTGTGTTTTA+TGG - Chr5:99603773-99603792 MsG0580029622.01.T01:CDS 45.0%
GAGTTCGCCAGATGAGAATC+AGG - Chr5:99603253-99603272 MsG0580029622.01.T01:CDS 50.0%
TCGGGTTTGTCAACGTCCAT+AGG + Chr5:99603680-99603699 None:intergenic 50.0%
TTCGCCAGATGAGAATCAGG+TGG - Chr5:99603256-99603275 MsG0580029622.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 99602498 99603814 99602498 ID=MsG0580029622.01;Name=MsG0580029622.01
Chr5 mRNA 99602498 99603814 99602498 ID=MsG0580029622.01.T01;Parent=MsG0580029622.01;Name=MsG0580029622.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|438
Chr5 exon 99602498 99603814 99602498 ID=MsG0580029622.01.T01:exon:36254;Parent=MsG0580029622.01.T01
Chr5 CDS 99602498 99603814 99602498 ID=MsG0580029622.01.T01:cds;Parent=MsG0580029622.01.T01
Gene Sequence

>MsG0580029622.01.T01

ATGGATTCATTCAATCAGAGTCATCAAAACAACAGCTTTAGGTACAATCCAAATTTGAATAGAGCTACACAAGTAGATGATGAAGATGAAGCAGAGTTCACAGGGCTTCTTGATGTCTATGTTCACCATGCTAGAAACATTCATAACATTTGCATCTATGATAATCAAGATGTATATGCTAAATTCTCCCTCACTTACAACCCTGATGAAACTCTTTCTACAAGAATAATCAATGGAGGTGGAAAAAATCCAACATTCAATGAAAACTTGAGGATGAAGATAACTCAAATAGATGCTGTTATGAAATGTGAAATTTGGATGTTTAGTAGAGCAAGAATTCACATGGAAGATCAACTTTTAGGGTTTGCTTTGGTCCCAATTTCACAAATTGTTGGAAAAGGAAAAGTAACTGAAGACTATAGCCTTTCTTCAACTGATCTTTTTCATTCTCCAGCTGGAACTGTTCAATTAACATTATCTTTAGACACATCTTTGGTATCAGAATCAACAAATAGTTCGTCGATATCGTCAGAAGTTATTTTGCTTGATAGAAAGATATCTGAAGTTATGTTGGATCCAATTGAGTATTCAAGAATTGAATTTCCTGACATAAGTATTGTTAAGGAGAATCAACAAATGGTTTCTCAATACTTCAATCTTGCTTGTGCCTCAAGTAACAACAATTCAAGAAAGTTGCTGCCATTTCTTCATCTTGGTGCTTCTCATCAACTTGATGATTATGAAATGATGACTATGAGTTCGCCAGATGAGAATCAGGTGGATTCGATCTCTCCCAATGAAAGCCTTCACAATTCCGGTTTGGTAAGCTCTACTATCACAAGCTTAAGCGACGATAGAAATTCCGCAGACTCGTTTGAGAAAAAGAATCATCTAGGATGTGATTCAACCAATTTTGTTACTCTTTGTCCCGATACACCAACTTCAAAGAAAGAAGGTGATCAATCAAAAGATGATGAGAAAGAAGAAAAATTCTCAAACAAAGAAAAGGAATGCAAGAAGGAAAGGAGCATAGAAGTAACAAAGTTTGGACAAGTTTTTAATTCTCCATTAGGGAATATTAATCTTGAAGCTGAGGAATGTGCTATGCAGAAGCAAATAGTGGATATGTATATGAGGAGCATGCAACAGTTTACTGAGTCCTTGGCGAAAATGAAGCTTCCTATGGACGTTGACAAACCCGAAAGACAAGATCATAGCGGTGATGTGATTCCAAATCATGAGAACAAGAAGTTAGAAATCGACAATAAGAAAAAGGATGGTTCGCGTGTGTTTTATGGTAGTCGAGCGTTTTTCTAA

Protein sequence

>MsG0580029622.01.T01

MDSFNQSHQNNSFRYNPNLNRATQVDDEDEAEFTGLLDVYVHHARNIHNICIYDNQDVYAKFSLTYNPDETLSTRIINGGGKNPTFNENLRMKITQIDAVMKCEIWMFSRARIHMEDQLLGFALVPISQIVGKGKVTEDYSLSSTDLFHSPAGTVQLTLSLDTSLVSESTNSSSISSEVILLDRKISEVMLDPIEYSRIEFPDISIVKENQQMVSQYFNLACASSNNNSRKLLPFLHLGASHQLDDYEMMTMSSPDENQVDSISPNESLHNSGLVSSTITSLSDDRNSADSFEKKNHLGCDSTNFVTLCPDTPTSKKEGDQSKDDEKEEKFSNKEKECKKERSIEVTKFGQVFNSPLGNINLEAEECAMQKQIVDMYMRSMQQFTESLAKMKLPMDVDKPERQDHSGDVIPNHENKKLEIDNKKKDGSRVFYGSRAFF*