AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017540.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017540.01.T01 MTR_3g108770 91.429 140 7 2 1 136 4 142 6.00e-89 255
MsG0380017540.01.T01 MTR_1g045900 90.714 140 8 2 1 136 4 142 1.98e-88 253
MsG0380017540.01.T01 MTR_4g075190 84.286 140 17 2 1 136 4 142 5.24e-84 242
MsG0380017540.01.T01 MTR_5g075850 74.074 54 12 1 85 136 1 54 1.24e-22 84.3
MsG0380017540.01.T01 MTR_5g075820 71.111 45 13 0 92 136 110 154 2.17e-17 73.9
MsG0380017540.01.T01 MTR_1g054195 68.889 45 13 1 92 135 175 219 6.59e-16 71.6
MsG0380017540.01.T01 MTR_1g080840 31.667 120 73 4 1 113 4 121 7.77e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017540.01.T01 AT5G08290 88.571 140 11 2 1 136 4 142 4.90e-87 250
MsG0380017540.01.T01 AT5G08290 97.059 102 3 0 35 136 1 102 3.79e-71 208
MsG0380017540.01.T01 AT3G24730 36.458 96 55 3 36 127 50 143 8.29e-14 64.7

Find 54 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACAGTTATGTTCTTCTTT+AGG 0.229575 3:-97640101 MsG0380017540.01.T01:CDS
GTACCAAATGAGGTAGATTT+TGG 0.235671 3:+97639845 None:intergenic
CATAAAACATAAATCTTTGT+TGG 0.327339 3:+97639704 None:intergenic
GTACTTGGTGGAGTAATCTT+TGG 0.329915 3:+97639939 None:intergenic
GACAGTTACACACTTGATAA+AGG 0.331693 3:+97639820 None:intergenic
GAAACAACAACAAGATCAAT+TGG 0.342414 3:-97640047 MsG0380017540.01.T01:CDS
TGTCCAAAATCTACCTCATT+TGG 0.347328 3:-97639848 MsG0380017540.01.T01:three_prime_UTR
GCCTCACTTGCATTCGGGAT+GGG 0.362710 3:-97641015 MsG0380017540.01.T01:CDS
CATGCTGCCTCACTTGCATT+CGG 0.374586 3:-97641021 MsG0380017540.01.T01:CDS
TTCGGGATGGGCTGTTGATC+AGG 0.384531 3:-97641003 MsG0380017540.01.T01:CDS
GTCATCCGTTTGGTCACGAT+TGG 0.401871 3:-97640951 MsG0380017540.01.T01:CDS
TACTTGGTGGAGTAATCTTT+GGG 0.403435 3:+97639940 None:intergenic
TTGGGCTATGAAGGACAAAC+AGG 0.451623 3:-97640028 MsG0380017540.01.T01:CDS
CAAGATCAATTGGGCTATGA+AGG 0.451707 3:-97640037 MsG0380017540.01.T01:CDS
TACATTCTAGTAACGGTACT+TGG 0.457670 3:+97639924 None:intergenic
TGCCTCACTTGCATTCGGGA+TGG 0.468994 3:-97641016 MsG0380017540.01.T01:CDS
TGTTGTTGTTTCCAGTTCCA+AGG 0.469561 3:+97640058 None:intergenic
TCATCCGTTTGGTCACGATT+GGG 0.473676 3:-97640950 MsG0380017540.01.T01:CDS
ACAGTTACACACTTGATAAA+GGG 0.481529 3:+97639821 None:intergenic
TCTACAGAGGTGCAAGGGAG+GGG 0.484596 3:-97639981 MsG0380017540.01.T01:CDS
GTGTATGCCCAACATGTACT+TGG 0.485312 3:-97639736 MsG0380017540.01.T01:three_prime_UTR
ATCATGATAGACCTTGGAAC+TGG 0.486256 3:-97640069 MsG0380017540.01.T01:CDS
ATGCTGCCTCACTTGCATTC+GGG 0.490375 3:-97641020 MsG0380017540.01.T01:CDS
CACATAATCCAAGTACATGT+TGG 0.495823 3:+97639728 None:intergenic
ACTAGAATGTATCAATCTAC+AGG 0.497165 3:-97639912 MsG0380017540.01.T01:exon
ACATAATCCAAGTACATGTT+GGG 0.503365 3:+97639729 None:intergenic
AGAGGTGCAAGGGAGGGGCG+TGG 0.504679 3:-97639976 MsG0380017540.01.T01:CDS
ACGTCTCGTTGTCATCCGTT+TGG 0.505472 3:-97640961 MsG0380017540.01.T01:CDS
AAACAACAACAAGATCAATT+GGG 0.514098 3:-97640046 MsG0380017540.01.T01:CDS
TCATACATAGTGTTAAAGTC+AGG 0.525607 3:+97640138 None:intergenic
AAGAAGAACATAACTGTGGA+AGG 0.528366 3:+97640105 None:intergenic
TCATCCCAATCGTGACCAAA+CGG 0.546375 3:+97640946 None:intergenic
GATAAAGGGTGTACCAAATG+AGG 0.558599 3:+97639835 None:intergenic
GTCTACAGAGGTGCAAGGGA+GGG 0.559541 3:-97639982 MsG0380017540.01.T01:CDS
AAGCACATCATGATAGACCT+TGG 0.567283 3:-97640075 MsG0380017540.01.T01:CDS
GACTGTCTACAGAGGTGCAA+GGG 0.580642 3:-97639986 MsG0380017540.01.T01:CDS
ATTCTAGTAACGGTACTTGG+TGG 0.586412 3:+97639927 None:intergenic
TCAGGCAATCCTCGCTGAGG+AGG 0.599877 3:-97640985 MsG0380017540.01.T01:CDS
GTTTAAGATGACATACAAGT+AGG 0.612858 3:+97639776 None:intergenic
GCCCATCCCGAATGCAAGTG+AGG 0.615016 3:+97641014 None:intergenic
TGTCTACAGAGGTGCAAGGG+AGG 0.615983 3:-97639983 MsG0380017540.01.T01:CDS
GATGGATGAGGTGCTGTCAT+CGG 0.623483 3:-97640217 MsG0380017540.01.T01:CDS
GGTGTGTGTCAAGATGGATG+AGG 0.631234 3:-97640229 MsG0380017540.01.T01:intron
AGAAGAACATAACTGTGGAA+GGG 0.633856 3:+97640106 None:intergenic
GCACCTCTGTGATGTCCACA+AGG 0.642857 3:+97640160 None:intergenic
AGACTGTCTACAGAGGTGCA+AGG 0.646760 3:-97639987 MsG0380017540.01.T01:CDS
GAATGTATCAATCTACAGGA+AGG 0.651779 3:-97639908 MsG0380017540.01.T01:three_prime_UTR
CTTTGCTGTGATATACCTTG+TGG 0.662344 3:-97640175 MsG0380017540.01.T01:CDS
AGATTGATACATTCTAGTAA+CGG 0.666479 3:+97639917 None:intergenic
CCTAAAGAAGAACATAACTG+TGG 0.685302 3:+97640101 None:intergenic
TGATCAGGCAATCCTCGCTG+AGG 0.692917 3:-97640988 MsG0380017540.01.T01:CDS
ATCATTGAGACTGTCTACAG+AGG 0.717834 3:-97639994 MsG0380017540.01.T01:CDS
CGAGACGTTCCTCCTCAGCG+AGG 0.755149 3:+97640976 None:intergenic
ATACCTTGTGGACATCACAG+AGG 0.789529 3:-97640163 MsG0380017540.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGCTTTTTTTTTTTTTTTTT+TGG - Chr3:97640461-97640480 MsG0380017540.01.T01:intron 10.0%
!! AAAACAGCCAAATAATATAT+AGG - Chr3:97640014-97640033 MsG0380017540.01.T01:CDS 20.0%
!! AGAGATAAAGTAATGAATTT+AGG - Chr3:97640044-97640063 MsG0380017540.01.T01:CDS 20.0%
!! ATCAACCTAAAATTAAGAAT+TGG + Chr3:97640387-97640406 None:intergenic 20.0%
!! CATAAAACATAAATCTTTGT+TGG + Chr3:97641010-97641029 None:intergenic 20.0%
!! CTTATGTCCTATATATTATT+TGG + Chr3:97640024-97640043 None:intergenic 20.0%
!! GAGATAAAGTAATGAATTTA+GGG - Chr3:97640045-97640064 MsG0380017540.01.T01:CDS 20.0%
!! TAAAATATCAAGAATGCTTA+TGG - Chr3:97639865-97639884 MsG0380017540.01.T01:three_prime_UTR 20.0%
!! TCAACCTAAAATTAAGAATT+GGG + Chr3:97640386-97640405 None:intergenic 20.0%
!!! TCTTAATTTTAGGTTGATAA+GGG - Chr3:97640389-97640408 MsG0380017540.01.T01:intron 20.0%
!!! TTCTTAATTTTAGGTTGATA+AGG - Chr3:97640388-97640407 MsG0380017540.01.T01:intron 20.0%
! AAACAACAACAAGATCAATT+GGG - Chr3:97640665-97640684 MsG0380017540.01.T01:intron 25.0%
! AGATTGATACATTCTAGTAA+CGG + Chr3:97640797-97640816 None:intergenic 25.0%
! ATAAAGTAATGAATTTAGGG+AGG - Chr3:97640048-97640067 MsG0380017540.01.T01:CDS 25.0%
! CAACCTAAAATTAAGAATTG+GGG + Chr3:97640385-97640404 None:intergenic 25.0%
! CAGCATAAACAATTTGAATT+TGG + Chr3:97640093-97640112 None:intergenic 25.0%
!! ACAAAACTGCTTAATTTGAA+TGG - Chr3:97639898-97639917 MsG0380017540.01.T01:three_prime_UTR 25.0%
AATATCAAGAATGCTTATGG+AGG - Chr3:97639868-97639887 MsG0380017540.01.T01:three_prime_UTR 30.0%
ACAGTTACACACTTGATAAA+GGG + Chr3:97640893-97640912 None:intergenic 30.0%
ACATAATCCAAGTACATGTT+GGG + Chr3:97640985-97641004 None:intergenic 30.0%
ACCATCACAATAACAATTTC+AGG - Chr3:97640285-97640304 MsG0380017540.01.T01:intron 30.0%
ACTAGAATGTATCAATCTAC+AGG - Chr3:97640799-97640818 MsG0380017540.01.T01:intron 30.0%
ATTGAGCGAAACTGATAATT+AGG - Chr3:97640416-97640435 MsG0380017540.01.T01:intron 30.0%
GAAACAACAACAAGATCAAT+TGG - Chr3:97640664-97640683 MsG0380017540.01.T01:intron 30.0%
TCATACATAGTGTTAAAGTC+AGG + Chr3:97640576-97640595 None:intergenic 30.0%
TGATGTATTTACAGAGAAAC+AGG + Chr3:97640183-97640202 None:intergenic 30.0%
! ATGCCCCAATTCTTAATTTT+AGG - Chr3:97640379-97640398 MsG0380017540.01.T01:intron 30.0%
! GTTTAAGATGACATACAAGT+AGG + Chr3:97640938-97640957 None:intergenic 30.0%
! TCCTGAAATTGTTATTGTGA+TGG + Chr3:97640289-97640308 None:intergenic 30.0%
AAGAAGAACATAACTGTGGA+AGG + Chr3:97640609-97640628 None:intergenic 35.0%
AAGTAATGAATTTAGGGAGG+TGG - Chr3:97640051-97640070 MsG0380017540.01.T01:CDS 35.0%
AGAAGAACATAACTGTGGAA+GGG + Chr3:97640608-97640627 None:intergenic 35.0%
AGGACTATATTGGGAAATAC+TGG + Chr3:97640139-97640158 None:intergenic 35.0%
CACATAATCCAAGTACATGT+TGG + Chr3:97640986-97641005 None:intergenic 35.0%
CCACAGTTATGTTCTTCTTT+AGG - Chr3:97640610-97640629 MsG0380017540.01.T01:intron 35.0%
CCTAAAGAAGAACATAACTG+TGG + Chr3:97640613-97640632 None:intergenic 35.0%
GAATGTATCAATCTACAGGA+AGG - Chr3:97640803-97640822 MsG0380017540.01.T01:intron 35.0%
GACAGTTACACACTTGATAA+AGG + Chr3:97640894-97640913 None:intergenic 35.0%
TACATTCTAGTAACGGTACT+TGG + Chr3:97640790-97640809 None:intergenic 35.0%
TGTCCAAAATCTACCTCATT+TGG - Chr3:97640863-97640882 MsG0380017540.01.T01:intron 35.0%
TTCAGAAAAAGTTGAGAAGC+AGG + Chr3:97640159-97640178 None:intergenic 35.0%
TTGAGAAGCAGGACTATATT+GGG + Chr3:97640148-97640167 None:intergenic 35.0%
! GTACCAAATGAGGTAGATTT+TGG + Chr3:97640869-97640888 None:intergenic 35.0%
! TACTTGGTGGAGTAATCTTT+GGG + Chr3:97640774-97640793 None:intergenic 35.0%
!!! TTTTTTGGTGTGTGTCAAGA+TGG - Chr3:97640476-97640495 MsG0380017540.01.T01:intron 35.0%
AAGCACATCATGATAGACCT+TGG - Chr3:97640636-97640655 MsG0380017540.01.T01:intron 40.0%
ATCATGATAGACCTTGGAAC+TGG - Chr3:97640642-97640661 MsG0380017540.01.T01:intron 40.0%
ATCATTGAGACTGTCTACAG+AGG - Chr3:97640717-97640736 MsG0380017540.01.T01:intron 40.0%
ATTCTAGTAACGGTACTTGG+TGG + Chr3:97640787-97640806 None:intergenic 40.0%
CAAGATCAATTGGGCTATGA+AGG - Chr3:97640674-97640693 MsG0380017540.01.T01:intron 40.0%
GTTGAGAAGCAGGACTATAT+TGG + Chr3:97640149-97640168 None:intergenic 40.0%
! CTTTGCTGTGATATACCTTG+TGG - Chr3:97640536-97640555 MsG0380017540.01.T01:intron 40.0%
! GATAAAGGGTGTACCAAATG+AGG + Chr3:97640879-97640898 None:intergenic 40.0%
! GTACTTGGTGGAGTAATCTT+TGG + Chr3:97640775-97640794 None:intergenic 40.0%
!! TGTTGTTGTTTCCAGTTCCA+AGG + Chr3:97640656-97640675 None:intergenic 40.0%
ATACCTTGTGGACATCACAG+AGG - Chr3:97640548-97640567 MsG0380017540.01.T01:intron 45.0%
GTGTATGCCCAACATGTACT+TGG - Chr3:97640975-97640994 MsG0380017540.01.T01:CDS 45.0%
TCATCCCAATCGTGACCAAA+CGG + Chr3:97639768-97639787 None:intergenic 45.0%
TCATCCGTTTGGTCACGATT+GGG - Chr3:97639761-97639780 MsG0380017540.01.T01:three_prime_UTR 45.0%
TTGGGATGAGACTTGTATGC+AGG - Chr3:97639779-97639798 MsG0380017540.01.T01:three_prime_UTR 45.0%
TTGGGCTATGAAGGACAAAC+AGG - Chr3:97640683-97640702 MsG0380017540.01.T01:intron 45.0%
ACGTCTCGTTGTCATCCGTT+TGG - Chr3:97639750-97639769 MsG0380017540.01.T01:three_prime_UTR 50.0%
ATACCACGAACACACTGCGA+TGG - Chr3:97640321-97640340 MsG0380017540.01.T01:intron 50.0%
ATACCATCGCAGTGTGTTCG+TGG + Chr3:97640327-97640346 None:intergenic 50.0%
ATGCTGCCTCACTTGCATTC+GGG - Chr3:97639691-97639710 MsG0380017540.01.T01:three_prime_UTR 50.0%
GGTGTGTGTCAAGATGGATG+AGG - Chr3:97640482-97640501 MsG0380017540.01.T01:intron 50.0%
GTCATCCGTTTGGTCACGAT+TGG - Chr3:97639760-97639779 MsG0380017540.01.T01:three_prime_UTR 50.0%
! AGACTGTCTACAGAGGTGCA+AGG - Chr3:97640724-97640743 MsG0380017540.01.T01:intron 50.0%
! GACTGTCTACAGAGGTGCAA+GGG - Chr3:97640725-97640744 MsG0380017540.01.T01:intron 50.0%
!! GATGGATGAGGTGCTGTCAT+CGG - Chr3:97640494-97640513 MsG0380017540.01.T01:intron 50.0%
GCACCTCTGTGATGTCCACA+AGG + Chr3:97640554-97640573 None:intergenic 55.0%
GCCTCACTTGCATTCGGGAT+GGG - Chr3:97639696-97639715 MsG0380017540.01.T01:three_prime_UTR 55.0%
TGATCAGGCAATCCTCGCTG+AGG - Chr3:97639723-97639742 MsG0380017540.01.T01:three_prime_UTR 55.0%
TGCCTCACTTGCATTCGGGA+TGG - Chr3:97639695-97639714 MsG0380017540.01.T01:three_prime_UTR 55.0%
! GTCTACAGAGGTGCAAGGGA+GGG - Chr3:97640729-97640748 MsG0380017540.01.T01:intron 55.0%
! TCTACAGAGGTGCAAGGGAG+GGG - Chr3:97640730-97640749 MsG0380017540.01.T01:intron 55.0%
! TGTCTACAGAGGTGCAAGGG+AGG - Chr3:97640728-97640747 MsG0380017540.01.T01:intron 55.0%
!! TTCGGGATGGGCTGTTGATC+AGG - Chr3:97639708-97639727 MsG0380017540.01.T01:three_prime_UTR 55.0%
GCCCATCCCGAATGCAAGTG+AGG + Chr3:97639700-97639719 None:intergenic 60.0%
TCAGGCAATCCTCGCTGAGG+AGG - Chr3:97639726-97639745 MsG0380017540.01.T01:three_prime_UTR 60.0%
CGAGACGTTCCTCCTCAGCG+AGG + Chr3:97639738-97639757 None:intergenic 65.0%
! AGAGGTGCAAGGGAGGGGCG+TGG - Chr3:97640735-97640754 MsG0380017540.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 97639691 97641042 97639691 ID=MsG0380017540.01;Name=MsG0380017540.01
Chr3 mRNA 97639691 97641042 97639691 ID=MsG0380017540.01.T01;Parent=MsG0380017540.01;Name=MsG0380017540.01.T01;_AED=0.31;_eAED=0.35;_QI=0|0|0.5|1|1|1|2|239|136
Chr3 exon 97639691 97640238 97639691 ID=MsG0380017540.01.T01:exon:5288;Parent=MsG0380017540.01.T01
Chr3 exon 97640941 97641042 97640941 ID=MsG0380017540.01.T01:exon:5287;Parent=MsG0380017540.01.T01
Chr3 CDS 97640941 97641042 97640941 ID=MsG0380017540.01.T01:cds;Parent=MsG0380017540.01.T01
Chr3 CDS 97639930 97640238 97639930 ID=MsG0380017540.01.T01:cds;Parent=MsG0380017540.01.T01
Chr3 three_prime_UTR 97639691 97639929 97639691 ID=MsG0380017540.01.T01:three_prime_utr;Parent=MsG0380017540.01.T01
Gene Sequence

>MsG0380017540.01.T01

ATGCTGCCTCACTTGCATTCGGGATGGGCTGTTGATCAGGCAATCCTCGCTGAGGAGGAACGTCTCGTTGTCATCCGTTTGGTCACGATTGGGATGAGACTTATGGATGAGGTGCTGTCATCGGTTGCAGAGACAATAAAGAACTTTGCTGTGATATACCTTGTGGACATCACAGAGGTGCCTGACTTTAACACTATGTATGAGCTGTATGACCCTTCCACAGTTATGTTCTTCTTTAGGAACAAGCACATCATGATAGACCTTGGAACTGGAAACAACAACAAGATCAATTGGGCTATGAAGGACAAACAGGAGTTCATTGACATCATTGAGACTGTCTACAGAGGTGCAAGGGAGGGGCGTGGTCTTGTCATTGCTCCCAAAGATTACTCCACCAAGTACCGTTACTAG

Protein sequence

>MsG0380017540.01.T01

MLPHLHSGWAVDQAILAEEERLVVIRLVTIGMRLMDEVLSSVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIMIDLGTGNNNKINWAMKDKQEFIDIIETVYRGAREGRGLVIAPKDYSTKYRY*