AlfalfaGEDB | MsG0480022166.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022166.01

KEGG: -
GO: GO:0005524
PFAM: PF00118

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022166.01.T01	AES90090.1	85.614	285	33	2	35	311	77	361	3.93E-164	475

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022166.01.T01	Q56XV8	65.614	285	89	3	35	310	183	467	6.40E-113	341

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022166.01.T01	Q2MGR4	85.614	285	33	2	35	311	189	473	1.72e-162	475

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0480022166.01	0.806230	9.097970e-50	4.912357e-47
MsG0080048748.01	MsG0480022166.01	0.831483	1.627469e-55	1.768474e-52
MsG0080048773.01	MsG0480022166.01	0.829654	4.561511e-55	4.698297e-52
MsG0080048826.01	MsG0480022166.01	0.811162	8.035754e-51	4.938029e-48
MsG0080048849.01	MsG0480022166.01	0.830462	2.897416e-55	3.054447e-52
MsG0080048938.01	MsG0480022166.01	0.882075	1.411085e-70	8.476203e-67
MsG0080049011.01	MsG0480022166.01	0.829451	5.111603e-55	5.233399e-52
MsG0080049068.01	MsG0480022166.01	0.801792	7.618179e-49	3.670855e-46
MsG0080049069.01	MsG0480022166.01	0.825586	4.324454e-54	3.958276e-51
MsG0180000026.01	MsG0480022166.01	0.842922	1.922703e-58	2.959357e-55

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022166.01.T01	MTR_4g084130	85.614	285	33	2	35	311	77	361	4.76e-168	475
MsG0480022166.01.T01	MTR_4g084130	85.614	285	33	2	35	311	189	473	4.15e-166	474
MsG0480022166.01.T01	MTR_4g057970	56.690	284	115	2	35	310	197	480	8.22e-104	315
MsG0480022166.01.T01	MTR_1g017390	40.426	282	160	2	35	308	197	478	2.58e-64	213
MsG0480022166.01.T01	MTR_1g017380	40.426	282	160	2	35	308	197	478	3.32e-64	213
MsG0480022166.01.T01	MTR_1g090130	37.638	271	155	5	37	296	185	452	3.84e-49	172
MsG0480022166.01.T01	MTR_1g090140	36.296	270	160	3	37	296	185	452	8.50e-49	171
MsG0480022166.01.T01	MTR_8g017080	33.688	282	175	3	41	312	190	469	1.47e-44	160

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022166.01.T01	AT5G18820	65.614	285	89	3	35	310	77	361	2.75e-115	341
MsG0480022166.01.T01	AT5G18820	65.614	285	89	3	35	310	183	467	6.53e-114	341
MsG0480022166.01.T01	AT2G28000	54.452	292	124	3	27	310	189	479	4.01e-101	308
MsG0480022166.01.T01	AT5G18820	63.229	223	80	1	35	255	183	405	6.44e-82	255
MsG0480022166.01.T01	AT1G55490	40.702	285	159	4	35	310	206	489	3.86e-64	213
MsG0480022166.01.T01	AT1G55490	40.702	285	159	4	35	310	206	489	3.86e-64	213
MsG0480022166.01.T01	AT1G55490	40.702	285	159	4	35	310	206	489	3.86e-64	213
MsG0480022166.01.T01	AT1G55490	40.702	285	159	4	35	310	206	489	3.86e-64	213
MsG0480022166.01.T01	AT1G55490	40.702	285	159	4	35	310	206	489	3.86e-64	213
MsG0480022166.01.T01	AT3G13470	40.351	285	160	4	35	310	202	485	1.43e-63	211

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Find 112 sgRNAs with CRISPR-Local

Find 371 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTAAGGCTGAAATTC+AGG	0.392021	4:-69210612	MsG0480022166.01.T01:CDS
AAATATAGTTGTTGCATTAC+TGG	0.223714	4:+69210643	None:intergenic
AAATCAATTGCAGCTAATGC+TGG	0.460888	4:-69207704	MsG0480022166.01.T01:three_prime_UTR
AACAAGAATGCTCTTATGAA+TGG	0.468016	4:-69207354	MsG0480022166.01.T01:three_prime_UTR
AACTTGAGGATAGAAAGCTG+AGG	0.724054	4:-69209206	MsG0480022166.01.T01:CDS
AATCGATACAATGCCATGAC+TGG	0.503792	4:-69207632	MsG0480022166.01.T01:three_prime_UTR
AATGAGGGACTTGTTCCTGG+AGG	0.707403	4:-69209147	MsG0480022166.01.T01:CDS
AATTGCATCGGAAAGCTATA+TGG	0.273514	4:+69212641	None:intergenic
ACACACTGAGCTAGAACTTG+AGG	0.625418	4:-69209220	MsG0480022166.01.T01:CDS
ACCAGAGGTACACGTGGCTT+GGG	0.437428	4:+69207478	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAACCATGAGCTACT+TGG	+	Chr4:69209884-69209903	None:intergenic	30.0%
AAAAATGTAAGCCTAGTATG+AGG	+	Chr4:69208521-69208540	None:intergenic	30.0%
AAAATACTAACGCTTGAGTA+GGG	-	Chr4:69211152-69211171	MsG0480022166.01.T01:intron	30.0%
AAAATTGAATCTTAGGGTGA+TGG	+	Chr4:69210184-69210203	None:intergenic	30.0%
AAACAGACAACAGCTATAAT+AGG	+	Chr4:69208132-69208151	None:intergenic	30.0%
AAATTGAATCTTAGGGTGAT+GGG	+	Chr4:69210183-69210202	None:intergenic	30.0%
AACAAGAATGCTCTTATGAA+TGG	-	Chr4:69212356-69212375	MsG0480022166.01.T01:intron	30.0%
AACAGACAACAGCTATAATA+GGG	+	Chr4:69208131-69208150	None:intergenic	30.0%
AGAAAAGGCAACATAATACA+TGG	+	Chr4:69210788-69210807	None:intergenic	30.0%
AGCAAGTCATTGTGTTTAAA+TGG	-	Chr4:69209919-69209938	MsG0480022166.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	69207045	69212687	69207045	ID=MsG0480022166.01;Name=MsG0480022166.01
Chr4	mRNA	69207045	69212687	69207045	ID=MsG0480022166.01.T01;Parent=MsG0480022166.01;Name=MsG0480022166.01.T01;_AED=0.27;_eAED=0.31;_QI=0\|0.33\|0.42\|0.85\|0.66\|0.57\|7\|713\|316
Chr4	exon	69212602	69212687	69212602	ID=MsG0480022166.01.T01:exon:8706;Parent=MsG0480022166.01.T01
Chr4	exon	69212075	69212219	69212075	ID=MsG0480022166.01.T01:exon:8707;Parent=MsG0480022166.01.T01
Chr4	exon	69211327	69211568	69211327	ID=MsG0480022166.01.T01:exon:8708;Parent=MsG0480022166.01.T01
Chr4	exon	69210952	69211010	69210952	ID=MsG0480022166.01.T01:exon:8709;Parent=MsG0480022166.01.T01
Chr4	exon	69210520	69210752	69210520	ID=MsG0480022166.01.T01:exon:8710;Parent=MsG0480022166.01.T01
Chr4	exon	69209052	69209250	69209052	ID=MsG0480022166.01.T01:exon:8711;Parent=MsG0480022166.01.T01
Chr4	exon	69207045	69207744	69207045	ID=MsG0480022166.01.T01:exon:8712;Parent=MsG0480022166.01.T01
Chr4	CDS	69212602	69212687	69212602	ID=MsG0480022166.01.T01:cds;Parent=MsG0480022166.01.T01
Chr4	CDS	69212075	69212219	69212075	ID=MsG0480022166.01.T01:cds;Parent=MsG0480022166.01.T01
Chr4	CDS	69211327	69211568	69211327	ID=MsG0480022166.01.T01:cds;Parent=MsG0480022166.01.T01
Chr4	CDS	69210952	69211010	69210952	ID=MsG0480022166.01.T01:cds;Parent=MsG0480022166.01.T01
Chr4	CDS	69210520	69210752	69210520	ID=MsG0480022166.01.T01:cds;Parent=MsG0480022166.01.T01
Chr4	CDS	69209065	69209250	69209065	ID=MsG0480022166.01.T01:cds;Parent=MsG0480022166.01.T01
Chr4	three_prime_UTR	69209052	69209064	69209052	ID=MsG0480022166.01.T01:three_prime_utr;Parent=MsG0480022166.01.T01
Chr4	three_prime_UTR	69207045	69207744	69207045	ID=MsG0480022166.01.T01:three_prime_utr;Parent=MsG0480022166.01.T01

Gene Sequence

>MsG0480022166.01.T01

ATGACGGCGTCACAATTGCACGACCCATATAGCTTTCCGATGCAATTGAGAATGCTGGTCCATGCTAATTCAAGAGGTCGACCATGCTGTAGCCTCGATTTCTGCTGGAATGACGAGTATGTTGGTAATTTGATTGCTGAAGCTATAGAGAAGATTGGATTTGATGGGGTAATCACCATCGAGTCATCCTCATCATCTGAACCCTCTGTGGTAATTGAAGAAGGGATGAAGATTGACAGGGGTTACATGTCTCCTTACTTCATCACCAACCAGGAAAAATCCATTGTGGAGTTTGACAATGCTAAAGTTTTGGTAACGGATCAGAAGATTTCAAGCGTCAAAGAAATAGTTCCTTTGCTGGAAAAAGCTATGCAGTTGAGTGCTCCGCTCTTAATTATTGCGGAGGACGTCACTGCACAAGTGTTGGAAACATTAATAGTGAACAAAATGCAAGATTACTACGAGTTGCAGCTTTGCCAAGATTACAATCCCCTAGCGGTTGTTTCAGTACGTCAGTTGACTGTTAATAGAGGTGCGGATTTTCTCTGTGGAGATTTGGGTCTTACACTTGAAGGTACAACATCAGACCAGCTTGGTACTGCAATGAAAGTGAAAATAACCAGTAATGCAACAACTATATTTGCTGATCCAAACACTAAGGCTGAAATTCAGGCTAGAATTTTACAGATAAAGAAGGATCTTATTGAAACAGACAATGCAAACCATTCGAGAAAGCTCTCAGAGAGAATTGCGAAACTCACTGGGGTAGGTGCACACACTGAGCTAGAACTTGAGGATAGAAAGCTGAGGATTGAGGATGCTAAGAATGCTACTTTTGCTGCCATAAATGAGGGACTTGTTCCTGGAGGGGGTGCCACATATGTCCACCTTTTGGACTTGATACCTGCCATAAAGAACTCCATGGAAGATCTGAGAGCAAATTGGTGCTGA

Protein sequence

>MsG0480022166.01.T01

MTASQLHDPYSFPMQLRMLVHANSRGRPCCSLDFCWNDEYVGNLIAEAIEKIGFDGVITIESSSSSEPSVVIEEGMKIDRGYMSPYFITNQEKSIVEFDNAKVLVTDQKISSVKEIVPLLEKAMQLSAPLLIIAEDVTAQVLETLIVNKMQDYYELQLCQDYNPLAVVSVRQLTVNRGADFLCGDLGLTLEGTTSDQLGTAMKVKITSNATTIFADPNTKAEIQARILQIKKDLIETDNANHSRKLSERIAKLTGVGAHTELELEDRKLRIEDAKNATFAAINEGLVPGGGATYVHLLDLIPAIKNSMEDLRANWC*