Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045644.01.T01 | GAU47077.1 | 85.294 | 102 | 10 | 1 | 1 | 97 | 1 | 102 | 9.18E-57 | 180 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045644.01.T01 | P41090 | 75.248 | 101 | 20 | 1 | 2 | 97 | 264 | 364 | 1.43E-48 | 160 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045644.01.T01 | A0A2Z6PN38 | 85.294 | 102 | 10 | 1 | 1 | 97 | 1 | 102 | 4.38e-57 | 180 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045644.01 | MsG0880045645.01 | 0.907284 | 5.982094e-81 | 1.054310e-76 |
| MsG0880045644.01 | MsG0880045960.01 | 0.815839 | 7.526738e-52 | 5.246390e-49 |
| MsG0180002808.01 | MsG0880045644.01 | 0.800308 | 1.531748e-48 | 7.109109e-46 |
| MsG0580024260.01 | MsG0880045644.01 | 0.805309 | 1.420479e-49 | 7.490168e-47 |
| MsG0580025023.01 | MsG0880045644.01 | 0.803429 | 3.501555e-49 | 1.759192e-46 |
| MsG0580028510.01 | MsG0880045644.01 | 0.800992 | 1.111105e-48 | 5.247222e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045644.01.T01 | MTR_8g075830 | 88.235 | 102 | 6 | 1 | 2 | 97 | 264 | 365 | 4.01e-58 | 182 |
| MsG0880045644.01.T01 | MTR_8g075890 | 86.275 | 102 | 8 | 1 | 2 | 97 | 264 | 365 | 1.91e-56 | 178 |
| MsG0880045644.01.T01 | MTR_3g058610 | 70.330 | 91 | 21 | 1 | 2 | 86 | 264 | 354 | 4.79e-40 | 135 |
| MsG0880045644.01.T01 | MTR_5g022020 | 82.090 | 67 | 12 | 0 | 4 | 70 | 266 | 332 | 4.75e-35 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045644.01.T01 | AT3G51240 | 69.792 | 96 | 29 | 0 | 2 | 97 | 179 | 274 | 1.23e-45 | 148 |
| MsG0880045644.01.T01 | AT3G51240 | 69.792 | 96 | 29 | 0 | 2 | 97 | 263 | 358 | 7.17e-45 | 148 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAATCTGGTGCTGGGTTT+TGG | 0.300278 | 8:+62098130 | None:intergenic |
| AAGAATCTCGTTCAATGGTT+TGG | 0.325008 | 8:+62097954 | None:intergenic |
| AGAATCTCGTTCAATGGTTT+GGG | 0.356018 | 8:+62097955 | None:intergenic |
| TACATTTCAGCAAAAGTGAT+TGG | 0.382508 | 8:+62098057 | None:intergenic |
| AAAGGGTACACAGTTGAATC+TGG | 0.445262 | 8:+62098117 | None:intergenic |
| GGAATGTTGCTATGGATAAA+CGG | 0.477243 | 8:+62098151 | None:intergenic |
| TAAGCAAGAATCTCGTTCAA+TGG | 0.477778 | 8:+62097949 | None:intergenic |
| TACACAGTTGAATCTGGTGC+TGG | 0.480189 | 8:+62098123 | None:intergenic |
| TCTCCATCTCTAATCTTCAA+AGG | 0.494530 | 8:+62098099 | None:intergenic |
| CTCCATCTCTAATCTTCAAA+GGG | 0.500771 | 8:+62098100 | None:intergenic |
| TATGGATAAACGGCTGTAGT+TGG | 0.512198 | 8:+62098161 | None:intergenic |
| CTAGGATGAAGAAGCTGGGT+AGG | 0.521316 | 8:-62098002 | MsG0880045644.01.T01:CDS |
| GATTGCTAGGATGAAGAAGC+TGG | 0.523859 | 8:-62098007 | MsG0880045644.01.T01:CDS |
| GTAAGGACCTTGAGATTGCT+AGG | 0.527971 | 8:-62098020 | MsG0880045644.01.T01:CDS |
| ACACAGTTGAATCTGGTGCT+GGG | 0.539512 | 8:+62098124 | None:intergenic |
| TACCCTTTGAAGATTAGAGA+TGG | 0.545506 | 8:-62098102 | MsG0880045644.01.T01:CDS |
| GTATAGGAGGAAGATGAGTA+AGG | 0.553968 | 8:-62098037 | MsG0880045644.01.T01:CDS |
| AGTTGGAATTCACCACTGCT+TGG | 0.567113 | 8:+62098178 | None:intergenic |
| TGTATGCAGTATCTAAGTAA+TGG | 0.572840 | 8:-62098222 | MsG0880045644.01.T01:CDS |
| TAGGATGAAGAAGCTGGGTA+GGG | 0.590624 | 8:-62098001 | MsG0880045644.01.T01:CDS |
| AGATGGAGAGAAATCTGTGA+TGG | 0.606446 | 8:-62098085 | MsG0880045644.01.T01:CDS |
| TGCAGTATCTAAGTAATGGA+CGG | 0.608604 | 8:-62098218 | MsG0880045644.01.T01:CDS |
| TCTTCATCCTAGCAATCTCA+AGG | 0.621342 | 8:+62098013 | None:intergenic |
| ATTGCTAGGATGAAGAAGCT+GGG | 0.635342 | 8:-62098006 | MsG0880045644.01.T01:CDS |
| AAATGCTGATCACCAAGCAG+TGG | 0.664697 | 8:-62098190 | MsG0880045644.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TACATTTCAGCAAAAGTGAT+TGG | + | Chr8:62098113-62098132 | None:intergenic | 30.0% | |
| !! | TCACTTTTGCTGAAATGTAT+AGG | - | Chr8:62098114-62098133 | MsG0880045644.01.T01:CDS | 30.0% |
| AAGAATCTCGTTCAATGGTT+TGG | + | Chr8:62098216-62098235 | None:intergenic | 35.0% | |
| AGAATCTCGTTCAATGGTTT+GGG | + | Chr8:62098215-62098234 | None:intergenic | 35.0% | |
| CTCCATCTCTAATCTTCAAA+GGG | + | Chr8:62098070-62098089 | None:intergenic | 35.0% | |
| GGAATGTTGCTATGGATAAA+CGG | + | Chr8:62098019-62098038 | None:intergenic | 35.0% | |
| TAAGCAAGAATCTCGTTCAA+TGG | + | Chr8:62098221-62098240 | None:intergenic | 35.0% | |
| TCTCCATCTCTAATCTTCAA+AGG | + | Chr8:62098071-62098090 | None:intergenic | 35.0% | |
| ! | CTTTTGCTGAAATGTATAGG+AGG | - | Chr8:62098117-62098136 | MsG0880045644.01.T01:CDS | 35.0% |
| ! | TACCCTTTGAAGATTAGAGA+TGG | - | Chr8:62098065-62098084 | MsG0880045644.01.T01:CDS | 35.0% |
| ! | TGCAGTATCTAAGTAATGGA+CGG | - | Chr8:62097949-62097968 | MsG0880045644.01.T01:CDS | 35.0% |
| !! | GCTCAAGTTTTGTCTTATGT+AGG | + | Chr8:62098193-62098212 | None:intergenic | 35.0% |
| AGATGGAGAGAAATCTGTGA+TGG | - | Chr8:62098082-62098101 | MsG0880045644.01.T01:CDS | 40.0% | |
| ATTGCTAGGATGAAGAAGCT+GGG | - | Chr8:62098161-62098180 | MsG0880045644.01.T01:CDS | 40.0% | |
| GTATAGGAGGAAGATGAGTA+AGG | - | Chr8:62098130-62098149 | MsG0880045644.01.T01:CDS | 40.0% | |
| TATGGATAAACGGCTGTAGT+TGG | + | Chr8:62098009-62098028 | None:intergenic | 40.0% | |
| TCTTCATCCTAGCAATCTCA+AGG | + | Chr8:62098157-62098176 | None:intergenic | 40.0% | |
| ! | AAAGGGTACACAGTTGAATC+TGG | + | Chr8:62098053-62098072 | None:intergenic | 40.0% |
| !!! | TGGGTTTTGGAATGTTGCTA+TGG | + | Chr8:62098027-62098046 | None:intergenic | 40.0% |
| AAATGCTGATCACCAAGCAG+TGG | - | Chr8:62097977-62097996 | MsG0880045644.01.T01:CDS | 45.0% | |
| ACACAGTTGAATCTGGTGCT+GGG | + | Chr8:62098046-62098065 | None:intergenic | 45.0% | |
| AGTTGGAATTCACCACTGCT+TGG | + | Chr8:62097992-62098011 | None:intergenic | 45.0% | |
| GATTGCTAGGATGAAGAAGC+TGG | - | Chr8:62098160-62098179 | MsG0880045644.01.T01:CDS | 45.0% | |
| GTAAGGACCTTGAGATTGCT+AGG | - | Chr8:62098147-62098166 | MsG0880045644.01.T01:CDS | 45.0% | |
| TACACAGTTGAATCTGGTGC+TGG | + | Chr8:62098047-62098066 | None:intergenic | 45.0% | |
| TAGGATGAAGAAGCTGGGTA+GGG | - | Chr8:62098166-62098185 | MsG0880045644.01.T01:CDS | 45.0% | |
| !! | TTGAATCTGGTGCTGGGTTT+TGG | + | Chr8:62098040-62098059 | None:intergenic | 45.0% |
| CTAGGATGAAGAAGCTGGGT+AGG | - | Chr8:62098165-62098184 | MsG0880045644.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 62097948 | 62098241 | 62097948 | ID=MsG0880045644.01;Name=MsG0880045644.01 |
| Chr8 | mRNA | 62097948 | 62098241 | 62097948 | ID=MsG0880045644.01.T01;Parent=MsG0880045644.01;Name=MsG0880045644.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|97 |
| Chr8 | exon | 62097948 | 62098241 | 62097948 | ID=MsG0880045644.01.T01:exon:23724;Parent=MsG0880045644.01.T01 |
| Chr8 | CDS | 62097948 | 62098241 | 62097948 | ID=MsG0880045644.01.T01:cds;Parent=MsG0880045644.01.T01 |
| Gene Sequence |
| Protein sequence |
