AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048177.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048177.01.T01 MTR_7g095710 98.810 84 1 0 1 84 748 831 5.72e-51 171
MsG0080048177.01.T01 MTR_6g016470 88.095 84 10 0 1 84 715 798 5.93e-45 154
MsG0080048177.01.T01 MTR_8g013780 85.714 84 12 0 1 84 267 350 1.11e-44 151
MsG0080048177.01.T01 MTR_2g038310 78.313 83 18 0 2 84 772 854 4.92e-38 134
MsG0080048177.01.T01 MTR_2g038310 78.313 83 18 0 2 84 772 854 5.15e-38 134
MsG0080048177.01.T01 MTR_4g008170 83.544 79 13 0 6 84 789 867 1.99e-37 133
MsG0080048177.01.T01 MTR_4g008170 83.544 79 13 0 6 84 796 874 2.35e-37 132
MsG0080048177.01.T01 MTR_2g105830 84.932 73 11 0 6 78 779 851 2.12e-34 124
MsG0080048177.01.T01 MTR_4g096990 69.412 85 25 1 1 84 716 800 3.64e-33 120
MsG0080048177.01.T01 MTR_5g015590 69.512 82 24 1 4 84 721 802 2.69e-32 118
MsG0080048177.01.T01 MTR_5g015590 69.512 82 24 1 4 84 721 802 2.77e-32 118
MsG0080048177.01.T01 MTR_5g015590 69.512 82 24 1 4 84 721 802 2.90e-32 118
MsG0080048177.01.T01 MTR_3g103070 67.059 85 27 1 1 84 544 628 1.50e-31 116
MsG0080048177.01.T01 MTR_3g103070 67.059 85 27 1 1 84 720 804 1.50e-31 116
MsG0080048177.01.T01 MTR_8g090125 63.415 82 29 1 4 84 676 757 6.62e-29 108
MsG0080048177.01.T01 MTR_4g043690 60.714 84 32 1 2 84 723 806 1.47e-28 107
MsG0080048177.01.T01 MTR_0005s0140 60.714 84 32 1 2 84 638 721 1.49e-28 107
MsG0080048177.01.T01 MTR_4g008650 62.195 82 30 1 4 84 718 799 2.13e-28 107
MsG0080048177.01.T01 MTR_4g008650 62.195 82 30 1 4 84 718 799 2.21e-28 107
MsG0080048177.01.T01 MTR_8g446010 45.833 96 51 1 4 98 674 769 3.20e-23 92.8
MsG0080048177.01.T01 MTR_8g045070 45.833 96 51 1 4 98 651 746 3.52e-23 92.4
MsG0080048177.01.T01 MTR_8g027160 54.667 75 34 0 8 82 702 776 1.17e-22 90.9
MsG0080048177.01.T01 MTR_8g027160 54.667 75 34 0 8 82 702 776 1.40e-22 90.9
MsG0080048177.01.T01 MTR_7g100110 60.000 70 28 0 9 78 719 788 4.98e-22 89.4
MsG0080048177.01.T01 MTR_5g015590 71.667 60 16 1 4 62 721 780 2.49e-20 84.3
MsG0080048177.01.T01 MTR_3g103270 46.154 91 47 2 4 93 691 780 7.68e-19 80.1
MsG0080048177.01.T01 MTR_8g027160 57.407 54 23 0 8 61 702 755 2.68e-14 67.4
MsG0080048177.01.T01 MTR_8g027160 55.556 54 24 0 8 61 702 755 7.54e-14 65.9
MsG0080048177.01.T01 MTR_2g036380 40.000 75 44 1 9 83 410 483 1.31e-12 62.4
MsG0080048177.01.T01 MTR_2g036380 40.000 75 44 1 9 83 379 452 1.33e-12 62.4
MsG0080048177.01.T01 MTR_2g036380 40.000 75 44 1 9 83 562 635 1.41e-12 62.4
MsG0080048177.01.T01 MTR_2g036380 40.000 75 44 1 9 83 472 545 1.45e-12 62.4
MsG0080048177.01.T01 MTR_8g079250 41.429 70 40 1 9 78 839 907 1.55e-12 62.4
MsG0080048177.01.T01 MTR_5g010420 41.892 74 42 1 9 82 830 902 2.89e-12 61.6
MsG0080048177.01.T01 MTR_8g012390 42.667 75 42 1 8 82 809 882 4.47e-12 60.8
MsG0080048177.01.T01 MTR_7g117500 36.842 95 52 2 2 96 561 647 4.51e-12 60.8
MsG0080048177.01.T01 MTR_2g035840 38.202 89 47 2 9 97 839 919 1.03e-11 59.7
MsG0080048177.01.T01 MTR_4g127580 38.667 75 45 1 9 83 569 642 1.06e-11 59.7
MsG0080048177.01.T01 MTR_4g094695 40.541 74 43 1 9 82 840 912 1.07e-11 59.7
MsG0080048177.01.T01 MTR_2g036650 35.417 96 54 2 2 97 561 648 1.37e-11 59.7
MsG0080048177.01.T01 MTR_4g116950 36.842 95 52 2 2 96 556 642 1.49e-11 59.3
MsG0080048177.01.T01 MTR_1g009760 35.789 95 53 2 2 96 555 641 1.75e-11 59.3
MsG0080048177.01.T01 MTR_1g009760 35.789 95 53 2 2 96 558 644 1.95e-11 58.9
MsG0080048177.01.T01 MTR_1g009720 34.737 95 54 2 2 96 555 641 2.02e-11 58.9
MsG0080048177.01.T01 MTR_2g104860 35.417 96 54 2 2 97 553 640 3.32e-11 58.5
MsG0080048177.01.T01 MTR_8g063880 34.737 95 54 2 2 96 555 641 3.59e-11 58.2
MsG0080048177.01.T01 MTR_2g449840 34.737 95 54 2 2 96 556 642 6.06e-11 57.8
MsG0080048177.01.T01 MTR_3g108800 34.737 95 54 2 2 96 555 641 7.82e-11 57.4
MsG0080048177.01.T01 MTR_3g108800 34.737 95 54 2 2 96 554 640 8.06e-11 57.4
MsG0080048177.01.T01 MTR_4g127710 34.375 96 55 2 2 97 558 645 8.22e-11 57.4
MsG0080048177.01.T01 MTR_4g127710 34.375 96 55 2 2 97 558 645 8.29e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048177.01.T01 AT3G63380 92.857 84 6 0 1 84 715 798 4.80e-47 160
MsG0080048177.01.T01 AT3G22910 86.905 84 11 0 1 84 711 794 9.90e-45 154
MsG0080048177.01.T01 AT5G57110 81.250 80 15 0 5 84 753 832 2.44e-38 135
MsG0080048177.01.T01 AT5G57110 81.250 80 15 0 5 84 753 832 2.44e-38 135
MsG0080048177.01.T01 AT5G57110 81.250 80 15 0 5 84 753 832 2.44e-38 135
MsG0080048177.01.T01 AT4G29900 77.500 80 18 0 5 84 757 836 2.51e-37 132
MsG0080048177.01.T01 AT4G29900 77.500 80 18 0 5 84 757 836 2.51e-37 132
MsG0080048177.01.T01 AT3G21180 82.278 79 14 0 6 84 771 849 9.86e-37 131
MsG0080048177.01.T01 AT4G37640 68.235 85 26 1 1 84 718 802 3.07e-32 118
MsG0080048177.01.T01 AT2G41560 67.901 81 26 0 4 84 716 796 3.36e-32 118
MsG0080048177.01.T01 AT2G41560 67.901 81 26 0 4 84 614 694 3.54e-32 118
MsG0080048177.01.T01 AT2G41560 67.901 81 26 0 4 84 614 694 3.54e-32 118
MsG0080048177.01.T01 AT2G41560 67.901 81 26 0 4 84 614 694 3.54e-32 118
MsG0080048177.01.T01 AT1G27770 65.882 85 28 1 1 84 647 731 1.51e-31 116
MsG0080048177.01.T01 AT1G27770 65.882 85 28 1 1 84 658 742 1.66e-31 116
MsG0080048177.01.T01 AT1G27770 65.882 85 28 1 1 84 721 805 1.85e-31 116
MsG0080048177.01.T01 AT1G27770 65.882 85 28 1 1 84 721 805 1.85e-31 116
MsG0080048177.01.T01 AT3G57330 66.667 81 27 0 4 84 658 738 1.92e-31 116
MsG0080048177.01.T01 AT3G57330 66.667 81 27 0 4 84 713 793 2.10e-31 115
MsG0080048177.01.T01 AT2G22950 67.059 85 27 1 1 84 719 803 2.76e-31 115
MsG0080048177.01.T01 AT5G53010 49.451 91 39 1 7 97 761 844 1.06e-23 94.0
MsG0080048177.01.T01 AT4G00900 54.667 75 34 0 8 82 698 772 1.72e-22 90.5
MsG0080048177.01.T01 AT4G00900 54.667 75 34 0 8 82 698 772 1.72e-22 90.5
MsG0080048177.01.T01 AT1G07670 58.571 70 29 0 9 78 703 772 7.19e-22 89.0
MsG0080048177.01.T01 AT1G07670 58.571 70 29 0 9 78 703 772 7.19e-22 89.0
MsG0080048177.01.T01 AT1G07810 58.571 70 29 0 9 78 703 772 8.90e-22 88.6
MsG0080048177.01.T01 AT1G10130 48.315 89 44 2 6 93 661 748 1.05e-19 82.8
MsG0080048177.01.T01 AT1G63440 44.595 74 40 1 9 82 838 910 5.59e-14 66.2
MsG0080048177.01.T01 AT4G30110 36.000 75 48 0 9 83 564 638 6.45e-12 60.5
MsG0080048177.01.T01 AT4G30110 36.000 75 48 0 9 83 564 638 6.45e-12 60.5
MsG0080048177.01.T01 AT1G17260 40.244 82 41 2 15 96 573 646 7.84e-12 60.5
MsG0080048177.01.T01 AT5G44790 41.892 74 42 1 9 82 849 921 9.64e-12 60.1
MsG0080048177.01.T01 AT2G19110 39.130 69 41 1 15 83 580 647 1.30e-11 59.7
MsG0080048177.01.T01 AT2G19110 39.130 69 41 1 15 83 580 647 1.30e-11 59.7
MsG0080048177.01.T01 AT2G19110 39.130 69 41 1 15 83 580 647 1.30e-11 59.7
MsG0080048177.01.T01 AT4G30190 35.417 96 54 2 2 97 553 640 1.45e-11 59.7
MsG0080048177.01.T01 AT2G18960 35.789 95 53 2 2 96 489 575 1.47e-11 59.3
MsG0080048177.01.T01 AT2G18960 35.789 95 53 2 2 96 489 575 1.47e-11 59.3
MsG0080048177.01.T01 AT2G18960 35.417 96 54 2 2 97 553 640 1.48e-11 59.3
MsG0080048177.01.T01 AT2G07560 35.417 96 54 2 2 97 555 642 1.57e-11 59.3
MsG0080048177.01.T01 AT2G24520 34.375 96 55 2 2 97 292 379 1.61e-11 59.3
MsG0080048177.01.T01 AT2G24520 34.375 96 55 2 2 97 535 622 1.85e-11 59.3
MsG0080048177.01.T01 AT2G24520 34.375 96 55 2 2 97 535 622 1.85e-11 59.3
MsG0080048177.01.T01 AT2G24520 34.375 96 55 2 2 97 598 685 1.89e-11 59.3
MsG0080048177.01.T01 AT3G42640 34.737 95 54 2 2 96 556 642 3.83e-11 58.2
MsG0080048177.01.T01 AT3G47950 34.375 96 55 2 2 97 561 648 5.61e-11 57.8
MsG0080048177.01.T01 AT3G47950 34.375 96 55 2 2 97 561 648 5.61e-11 57.8

Find 19 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAAACTGAATAAATTTC+TGG 0.116163 contig236end:+19990 None:intergenic
GACGATATCGGAACTTTCTT+TGG 0.231369 contig236end:+20080 None:intergenic
AGCTGATATAGGACTTTCTA+TGG 0.273764 contig236end:-20124 MsG0080048177.01.T01:CDS
CTTTGGCGACTTCGGTTCCT+TGG 0.332634 contig236end:+20097 None:intergenic
CCATCAAAAGTTTGTCCATA+GGG 0.437671 contig236end:+20223 None:intergenic
GGAACTTTCTTTGGCGACTT+CGG 0.449820 contig236end:+20089 None:intergenic
GCCTTAAAAGAAGCTGATAT+AGG 0.472466 contig236end:-20135 MsG0080048177.01.T01:CDS
GTGCAATGCTTAAGAAAGAA+AGG 0.495384 contig236end:-20201 MsG0080048177.01.T01:CDS
GGTCATGTAGTTGCGGTCAC+AGG 0.513331 contig236end:-20180 MsG0080048177.01.T01:CDS
GTCGTCTAAGATGACGATAT+CGG 0.571011 contig236end:+20068 None:intergenic
GGGATGATCTTGCCATCACT+CGG 0.576371 contig236end:+20244 None:intergenic
GATGGCAAGATCATCCCCTA+TGG 0.578067 contig236end:-20238 MsG0080048177.01.T01:CDS
GGACTTTCTATGGGAATCCA+AGG 0.578565 contig236end:-20114 MsG0080048177.01.T01:CDS
ACCATCAAAAGTTTGTCCAT+AGG 0.578755 contig236end:+20222 None:intergenic
AAAGAAAGGTCATGTAGTTG+CGG 0.598089 contig236end:-20187 MsG0080048177.01.T01:CDS
GCTGATATAGGACTTTCTAT+GGG 0.607580 contig236end:-20123 MsG0080048177.01.T01:CDS
AGTCGATAAGATCCGAGTGA+TGG 0.608832 contig236end:-20256 MsG0080048177.01.T01:CDS
GTAGTTGCGGTCACAGGCGA+CGG 0.616359 contig236end:-20174 MsG0080048177.01.T01:CDS
CATCAAAAGTTTGTCCATAG+GGG 0.646674 contig236end:+20224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTGAAACTGAATAAATTTC+TGG + contig236end:20264-20283 None:intergenic 25.0%
! ATTCTGTTGCAACTGTTTTA+AGG - contig236end:20213-20232 MsG0080048177.01.T01:CDS 30.0%
! TCCTATATCAGCTTCTTTTA+AGG + contig236end:20120-20139 None:intergenic 30.0%
AAAGAAAGGTCATGTAGTTG+CGG - contig236end:20064-20083 MsG0080048177.01.T01:CDS 35.0%
ACCATCAAAAGTTTGTCCAT+AGG + contig236end:20032-20051 None:intergenic 35.0%
AGCTGATATAGGACTTTCTA+TGG - contig236end:20127-20146 MsG0080048177.01.T01:CDS 35.0%
CATCAAAAGTTTGTCCATAG+GGG + contig236end:20030-20049 None:intergenic 35.0%
CCATCAAAAGTTTGTCCATA+GGG + contig236end:20031-20050 None:intergenic 35.0%
GCCTTAAAAGAAGCTGATAT+AGG - contig236end:20116-20135 MsG0080048177.01.T01:CDS 35.0%
GCTGATATAGGACTTTCTAT+GGG - contig236end:20128-20147 MsG0080048177.01.T01:CDS 35.0%
GTGCAATGCTTAAGAAAGAA+AGG - contig236end:20050-20069 MsG0080048177.01.T01:CDS 35.0%
!!! ATATCAGCTTCTTTTAAGGC+AGG + contig236end:20116-20135 None:intergenic 35.0%
!!! TGTTGCAACTGTTTTAAGGT+GGG - contig236end:20217-20236 MsG0080048177.01.T01:CDS 35.0%
GACGATATCGGAACTTTCTT+TGG + contig236end:20174-20193 None:intergenic 40.0%
! CCCTATGGACAAACTTTTGA+TGG - contig236end:20028-20047 MsG0080048177.01.T01:CDS 40.0%
! GTCGTCTAAGATGACGATAT+CGG + contig236end:20186-20205 None:intergenic 40.0%
!!! CTGTTGCAACTGTTTTAAGG+TGG - contig236end:20216-20235 MsG0080048177.01.T01:CDS 40.0%
AGTCGATAAGATCCGAGTGA+TGG - contig236end:19995-20014 MsG0080048177.01.T01:CDS 45.0%
GGAACTTTCTTTGGCGACTT+CGG + contig236end:20165-20184 None:intergenic 45.0%
GGACTTTCTATGGGAATCCA+AGG - contig236end:20137-20156 MsG0080048177.01.T01:CDS 45.0%
GATGGCAAGATCATCCCCTA+TGG - contig236end:20013-20032 MsG0080048177.01.T01:CDS 50.0%
GGGATGATCTTGCCATCACT+CGG + contig236end:20010-20029 None:intergenic 50.0%
CTTTGGCGACTTCGGTTCCT+TGG + contig236end:20157-20176 None:intergenic 55.0%
GGTCATGTAGTTGCGGTCAC+AGG - contig236end:20071-20090 MsG0080048177.01.T01:CDS 55.0%
GTAGTTGCGGTCACAGGCGA+CGG - contig236end:20077-20096 MsG0080048177.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
contig236end gene 19987 20286 19987 ID=MsG0080048177.01;Name=MsG0080048177.01
contig236end mRNA 19987 20286 19987 ID=MsG0080048177.01.T01;Parent=MsG0080048177.01;Name=MsG0080048177.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|99
contig236end exon 19987 20286 19987 ID=MsG0080048177.01.T01:exon:20250;Parent=MsG0080048177.01.T01
contig236end CDS 19987 20286 19987 ID=MsG0080048177.01.T01:cds;Parent=MsG0080048177.01.T01
Gene Sequence

>MsG0080048177.01.T01

ATGGAAAAAGTCGATAAGATCCGAGTGATGGCAAGATCATCCCCTATGGACAAACTTTTGATGGTGCAATGCTTAAGAAAGAAAGGTCATGTAGTTGCGGTCACAGGCGACGGCACAAACGATGCACCTGCCTTAAAAGAAGCTGATATAGGACTTTCTATGGGAATCCAAGGAACCGAAGTCGCCAAAGAAAGTTCCGATATCGTCATCTTAGACGACAACTTCAATTCTGTTGCAACTGTTTTAAGGTGGGAAGATGTTTTTACAACAACATCCAGAAATTTATTCAGTTTCAACTAA

Protein sequence

>MsG0080048177.01.T01

MEKVDKIRVMARSSPMDKLLMVQCLRKKGHVVAVTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVILDDNFNSVATVLRWEDVFTTTSRNLFSFN*