AlfalfaGEDB | MsG0880046716.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046716.01

KEGG: K07936
GO: GO:0003924 GO:0005525 GO:0006913
PFAM: PF00071

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046716.01.T01	XP_024637534.1	95.089	224	10	1	8	230	21	244	7.94E-158	447

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046716.01.T01	P38548	85.455	220	32	0	11	230	2	221	1.18E-142	400

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046716.01.T01	G7JV88	91.403	221	8	2	11	230	2	212	2.44e-145	413

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0880046716.01	MsG0880046726.01	0.959273	2.925188e-117	1.152825e-111

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PPI

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Gene1	Gene2	Type
MsG0480023941.01	MsG0880046716.01	PPI
MsG0580026742.01	MsG0880046716.01	PPI
MsG0780041238.01	MsG0880046716.01	PPI
MsG0880046716.01	MsG0280006351.01	PPI
MsG0880046716.01	MsG0480023719.01	PPI
MsG0880046716.01	MsG0880042987.01	PPI
MsG0880046716.01	MsG0880046390.01	PPI
MsG0880046716.01	MsG0180004652.01	PPI
MsG0880046716.01	MsG0580024324.01	PPI
MsG0880046716.01	MsG0180006070.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046716.01.T01	MTR_4g113620	91.403	221	8	2	11	230	2	212	6.18e-149	413
MsG0880046716.01.T01	MTR_3g107707	85.000	220	33	0	11	230	2	221	2.06e-143	400
MsG0880046716.01.T01	MTR_3g107713	84.545	220	34	0	11	230	2	221	7.43e-143	398
MsG0880046716.01.T01	MTR_3g107717	84.545	220	34	0	11	230	2	221	9.25e-143	398
MsG0880046716.01.T01	MTR_4g098950	30.570	193	125	4	23	212	10	196	3.82e-28	106
MsG0880046716.01.T01	MTR_5g013900	33.929	168	108	3	23	187	10	177	1.51e-27	104
MsG0880046716.01.T01	MTR_8g103550	34.783	161	102	3	24	181	11	171	2.60e-27	103
MsG0880046716.01.T01	MTR_8g031680	31.395	172	115	3	23	191	7	178	3.71e-27	103
MsG0880046716.01.T01	MTR_1g097420	28.877	187	120	4	23	196	9	195	7.18e-27	102
MsG0880046716.01.T01	MTR_3g064390	33.750	160	103	3	24	180	12	171	2.08e-26	101

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046716.01.T01	AT5G55190	84.545	220	34	0	11	230	2	221	1.00e-143	400
MsG0880046716.01.T01	AT5G20010	84.545	220	34	0	11	230	2	221	1.64e-142	397
MsG0880046716.01.T01	AT5G20020	85.000	220	33	0	11	230	2	221	1.07e-136	383
MsG0880046716.01.T01	AT5G55080	66.351	211	66	1	11	221	2	207	1.35e-100	291
MsG0880046716.01.T01	AT4G39890	36.585	164	100	3	23	182	10	173	4.12e-29	108
MsG0880046716.01.T01	AT2G44610	32.738	168	110	3	23	187	10	177	3.12e-27	103
MsG0880046716.01.T01	AT1G07410	32.086	187	119	3	23	206	13	194	8.68e-27	102
MsG0880046716.01.T01	AT5G39620	32.749	171	105	3	23	183	6	176	2.66e-26	101
MsG0880046716.01.T01	AT2G21880	31.361	169	106	3	19	177	6	174	5.28e-26	100
MsG0880046716.01.T01	AT4G18430	30.052	193	129	4	23	209	14	206	8.14e-26	100

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Find 46 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGCTACCATTGGTGTGG+AGG	0.584491	8:+77267994	MsG0880046716.01.T01:intron
AAGCATTTGAGTAAACTTTA+AGG	0.158330	8:+77269007	MsG0880046716.01.T01:exon
AAGCTTGTCCTTGTAGGCGA+TGG	0.503724	8:+77267679	MsG0880046716.01.T01:CDS
AATATACCCATTGTCCTGTG+TGG	0.680204	8:+77268491	MsG0880046716.01.T01:CDS
AATGCTTCATCATCGTCATC+AGG	0.493441	8:-77268991	None:intergenic
ACAAGGACAAGCTTGAAAGT+GGG	0.656622	8:-77267670	None:intergenic
ACCCTGCAAGTTTCTTGGCA+AGG	0.522554	8:-77268640	None:intergenic
ACCTTGCCAAGAAACTTGCA+GGG	0.657492	8:+77268639	MsG0880046716.01.T01:CDS
ACTTCCGGAGGAGCAAGAGC+AGG	0.529548	8:-77268793	None:intergenic
ACTTTCAAGCTTGTCCTTGT+AGG	0.329790	8:+77267673	MsG0880046716.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGTGAAGGTTCATATTAC+TGG	-	Chr8:77268764-77268783	None:intergenic	30.0%
AGCATAATTAAGTGGAACTT+TGG	-	Chr8:77267739-77267758	None:intergenic	30.0%
AGGTTCATATTACTGGAAAT+TGG	-	Chr8:77268757-77268776	None:intergenic	30.0%
CAAAGCAAGTTATATTTCAC+AGG	+	Chr8:77268549-77268568	MsG0880046716.01.T01:CDS	30.0%
TAATATGAACCTTCACTTTG+TGG	+	Chr8:77268765-77268784	MsG0880046716.01.T01:CDS	30.0%
TCTTAGCTGAGTGATAATTT+GGG	+	Chr8:77268431-77268450	MsG0880046716.01.T01:intron	30.0%
TGTGGAATCAATACGTAAAT+TGG	-	Chr8:77267917-77267936	None:intergenic	30.0%
TTAGACATGCATATATAGTG+TGG	-	Chr8:77267935-77267954	None:intergenic	30.0%
TTCTTAGCTGAGTGATAATT+TGG	+	Chr8:77268430-77268449	MsG0880046716.01.T01:intron	30.0%
CATACAGAAATGTTCCAACA+TGG	+	Chr8:77268323-77268342	MsG0880046716.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	77267610	77269214	77267610	ID=MsG0880046716.01;Name=MsG0880046716.01
Chr8	mRNA	77267610	77269214	77267610	ID=MsG0880046716.01.T01;Parent=MsG0880046716.01;Name=MsG0880046716.01.T01;_AED=0.27;_eAED=0.29;_QI=0\|0.66\|0.28\|1\|1\|1\|7\|194\|230
Chr8	exon	77267610	77267709	77267610	ID=MsG0880046716.01.T01:exon:32692;Parent=MsG0880046716.01.T01
Chr8	exon	77267834	77267890	77267834	ID=MsG0880046716.01.T01:exon:32693;Parent=MsG0880046716.01.T01
Chr8	exon	77267999	77268116	77267999	ID=MsG0880046716.01.T01:exon:32694;Parent=MsG0880046716.01.T01
Chr8	exon	77268273	77268362	77268273	ID=MsG0880046716.01.T01:exon:32695;Parent=MsG0880046716.01.T01
Chr8	exon	77268481	77268660	77268481	ID=MsG0880046716.01.T01:exon:32696;Parent=MsG0880046716.01.T01
Chr8	exon	77268765	77268842	77268765	ID=MsG0880046716.01.T01:exon:32697;Parent=MsG0880046716.01.T01
Chr8	exon	77268951	77269214	77268951	ID=MsG0880046716.01.T01:exon:32698;Parent=MsG0880046716.01.T01
Chr8	CDS	77267610	77267709	77267610	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	CDS	77267834	77267890	77267834	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	CDS	77267999	77268116	77267999	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	CDS	77268273	77268362	77268273	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	CDS	77268481	77268660	77268481	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	CDS	77268765	77268842	77268765	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	CDS	77268951	77269020	77268951	ID=MsG0880046716.01.T01:cds;Parent=MsG0880046716.01.T01
Chr8	three_prime_UTR	77269021	77269214	77269021	ID=MsG0880046716.01.T01:three_prime_utr;Parent=MsG0880046716.01.T01

Gene Sequence

>MsG0880046716.01.T01

ATGCTTTTGTTTTGTCCCGTTTCTATACAGGCTCTCCCTCAGCAGCAAATTGTTAACTATCCCACTTTCAAGCTTGTCCTTGTAGGCGATGGAGGAACTGGAAAAACCACTTTTGTTAAAAGACATCTAACTGGCGAATTTGAGAAGAGATATGAACCTACCATTGGTGTGGAGGTTCATCCACTAGACTTTTATACAAATTGTGGAAAAATTCGTTTTAATTGCTGGGACACTGCTGGCCAAGAAAAATTTGGTGGTCTCAGAGATGCTTATTACACTCAAGGACAATGTGCTATTATTATGTTCGATGTAACTGCTCGTTTGACATACAGAAATGTTCCAACATGGCACAGAGATATCACTCGTGTGTGTGAGAATATACCCATTGTCCTGTGTGGAAACAAGGTTGATGTGAAGAATAGACAGATTAAAGCAAAGCAAGTTATATTTCACAGGAAGAAGAATCTACAATACTATGAAATATCTGCAAAGAGTAACTATAATTTTGAGAAACCTTTCTTATACCTTGCCAAGAAACTTGCAGGTAATATGAACCTTCACTTTGTGGAGATGCCTGCTCTTGCTCCTCCGGAAGTCGCCTTTGATATAGCTGCGCAAGAATCGATTGAACATGAGATATGTATGCCAGCATTTCAGCCTCTTCCTGATGACGATGATGAAGCATTTGAGTAA

Protein sequence

>MsG0880046716.01.T01

MLLFCPVSIQALPQQQIVNYPTFKLVLVGDGGTGKTTFVKRHLTGEFEKRYEPTIGVEVHPLDFYTNCGKIRFNCWDTAGQEKFGGLRDAYYTQGQCAIIMFDVTARLTYRNVPTWHRDITRVCENIPIVLCGNKVDVKNRQIKAKQVIFHRKKNLQYYEISAKSNYNFEKPFLYLAKKLAGNMNLHFVEMPALAPPEVAFDIAAQESIEHEICMPAFQPLPDDDDEAFE*