Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046782.01.T01 | XP_003630728.1 | 85.544 | 754 | 63 | 5 | 14 | 724 | 14 | 764 | 0 | 1199 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000010.01 | MsG0880046782.01 | 0.808623 | 2.827507e-50 | 1.624449e-47 |
| MsG0180003246.01 | MsG0880046782.01 | 0.813417 | 2.586426e-51 | 1.688112e-48 |
| MsG0180003767.01 | MsG0880046782.01 | 0.804482 | 2.115497e-49 | 1.091951e-46 |
| MsG0180006034.01 | MsG0880046782.01 | 0.811220 | 7.805781e-51 | 4.804204e-48 |
| MsG0280011440.01 | MsG0880046782.01 | 0.817479 | 3.227992e-52 | 2.353514e-49 |
| MsG0380016347.01 | MsG0880046782.01 | 0.801888 | 7.278763e-49 | 3.516093e-46 |
| MsG0380016619.01 | MsG0880046782.01 | 0.813805 | 2.125609e-51 | 1.402002e-48 |
| MsG0480022872.01 | MsG0880046782.01 | 0.806635 | 7.474314e-50 | 4.077113e-47 |
| MsG0780039997.01 | MsG0880046782.01 | 0.814677 | 1.364086e-51 | 9.212022e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046782.01.T01 | MTR_8g102650 | 85.544 | 754 | 63 | 5 | 14 | 724 | 14 | 764 | 0.0 | 1199 |
| MsG0880046782.01.T01 | MTR_8g102650 | 32.314 | 229 | 95 | 9 | 559 | 731 | 655 | 879 | 3.98e-12 | 70.5 |
| MsG0880046782.01.T01 | MTR_7g069290 | 51.991 | 427 | 163 | 6 | 21 | 445 | 14 | 400 | 4.62e-139 | 415 |
| MsG0880046782.01.T01 | MTR_7g069270 | 50.000 | 460 | 168 | 8 | 1 | 458 | 1 | 400 | 5.44e-136 | 407 |
| MsG0880046782.01.T01 | MTR_3g091460 | 36.704 | 534 | 318 | 8 | 19 | 538 | 25 | 552 | 3.03e-95 | 309 |
| MsG0880046782.01.T01 | MTR_1g113010 | 33.945 | 545 | 340 | 8 | 8 | 538 | 12 | 550 | 1.66e-90 | 296 |
| MsG0880046782.01.T01 | MTR_7g069230 | 54.406 | 261 | 88 | 4 | 106 | 363 | 2 | 234 | 4.47e-85 | 269 |
| MsG0880046782.01.T01 | MTR_7g069300 | 53.846 | 156 | 63 | 2 | 174 | 329 | 47 | 193 | 3.57e-49 | 173 |
| MsG0880046782.01.T01 | MTR_7g069300 | 35.122 | 205 | 105 | 4 | 337 | 530 | 65 | 252 | 3.49e-31 | 123 |
| MsG0880046782.01.T01 | MTR_1g050495 | 26.777 | 422 | 299 | 3 | 116 | 537 | 125 | 536 | 1.33e-38 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046782.01.T01 | AT1G10270 | 65.125 | 519 | 174 | 2 | 25 | 539 | 86 | 601 | 0.0 | 690 |
| MsG0880046782.01.T01 | AT3G49240 | 36.347 | 542 | 328 | 11 | 7 | 538 | 15 | 549 | 2.72e-92 | 301 |
| MsG0880046782.01.T01 | AT3G60960 | 36.386 | 415 | 212 | 11 | 50 | 445 | 18 | 399 | 6.59e-67 | 226 |
| MsG0880046782.01.T01 | AT3G60960 | 36.386 | 415 | 212 | 11 | 50 | 445 | 18 | 399 | 6.59e-67 | 226 |
| MsG0880046782.01.T01 | AT3G60960 | 36.386 | 415 | 212 | 11 | 50 | 445 | 18 | 399 | 6.59e-67 | 226 |
| MsG0880046782.01.T01 | AT3G60980 | 38.196 | 377 | 201 | 11 | 89 | 441 | 38 | 406 | 3.52e-66 | 225 |
| MsG0880046782.01.T01 | AT5G28380 | 36.000 | 325 | 161 | 9 | 134 | 441 | 88 | 382 | 1.29e-52 | 187 |
| MsG0880046782.01.T01 | AT5G28340 | 36.000 | 325 | 161 | 9 | 134 | 441 | 133 | 427 | 2.31e-52 | 188 |
| MsG0880046782.01.T01 | AT5G28380 | 34.426 | 305 | 153 | 9 | 154 | 441 | 1 | 275 | 4.04e-43 | 157 |
| MsG0880046782.01.T01 | AT1G12620 | 26.357 | 387 | 274 | 4 | 115 | 501 | 174 | 549 | 6.93e-41 | 159 |
Find 222 sgRNAs with CRISPR-Local
Find 248 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGCTTAGAATTTATCT+TGG | 0.409265 | 8:+78046377 | None:intergenic |
| AAATCATGGTGCTGATTCAA+TGG | 0.410356 | 8:-78046808 | MsG0880046782.01.T01:CDS |
| AACAAAACATTACAAGTGGC+AGG | 0.541519 | 8:+78046582 | None:intergenic |
| AACCATGATATTAAAAGTAT+CGG | 0.480661 | 8:+78046461 | None:intergenic |
| AACCTTCCTAAAAGTATCAA+GGG | 0.509127 | 8:+78046404 | None:intergenic |
| AAGAAACGATATTCGGAACA+AGG | 0.602063 | 8:+78047018 | None:intergenic |
| AAGGAGCATGCGAGATGATG+TGG | 0.572690 | 8:+78046931 | None:intergenic |
| AATCATCGCTGCTATGTATC+GGG | 0.526652 | 8:-78047096 | MsG0880046782.01.T01:CDS |
| AATCGCTGTATTGTTTATGA+TGG | 0.530866 | 8:-78046708 | MsG0880046782.01.T01:CDS |
| AATTGAGAAGGGGACCTTGC+CGG | 0.415817 | 8:+78045799 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCGCTGTATTGTTTATGA+TGG | - | Chr8:78046047-78046066 | MsG0880046782.01.T01:CDS | 30.0% | |
| AGAGATAATATTACGATACC+CGG | + | Chr8:78046417-78046436 | None:intergenic | 30.0% | |
| ATTTGAGAAGGAAACTCATA+AGG | + | Chr8:78047235-78047254 | None:intergenic | 30.0% | |
| CAACATCTGATCAAACAATT+CGG | + | Chr8:78046237-78046256 | None:intergenic | 30.0% | |
| GATAGATGGTTATTTGAAAG+TGG | - | Chr8:78046535-78046554 | MsG0880046782.01.T01:CDS | 30.0% | |
| GGTATCGTAATATTATCTCT+AGG | - | Chr8:78046417-78046436 | MsG0880046782.01.T01:CDS | 30.0% | |
| TTAACAGCTTGAAAATTTGG+AGG | + | Chr8:78046272-78046291 | None:intergenic | 30.0% | |
| TTATGAGTTTCCTTCTCAAA+TGG | - | Chr8:78047234-78047253 | MsG0880046782.01.T01:CDS | 30.0% | |
| AACAAAACATTACAAGTGGC+AGG | + | Chr8:78046176-78046195 | None:intergenic | 35.0% | |
| AAGAAACGATATTCGGAACA+AGG | + | Chr8:78045740-78045759 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 78045291 | 78047486 | 78045291 | ID=MsG0880046782.01;Name=MsG0880046782.01 |
| Chr8 | mRNA | 78045291 | 78047486 | 78045291 | ID=MsG0880046782.01.T01;Parent=MsG0880046782.01;Name=MsG0880046782.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|731 |
| Chr8 | exon | 78045291 | 78047486 | 78045291 | ID=MsG0880046782.01.T01:exon:18615;Parent=MsG0880046782.01.T01 |
| Chr8 | CDS | 78045291 | 78047486 | 78045291 | ID=MsG0880046782.01.T01:cds;Parent=MsG0880046782.01.T01 |
| Gene Sequence |
>MsG0880046782.01.T01 ATGTCACTCTGCCGCCTTCTCCTCCGCTCTCTCCGCCGTCCCACAACCGTCTCTCCCATCTCCATCACCACCCGCTCCTTCGCATTCTCCTCCGCCGAAGAAGCAGCGGCAGAGCGACGCCGTCGTAAACGCCGTCTTCGCATCGAGCCTCCCTTGAACGCAATCCGTCCTCCTCCTCCTCAACACCAGCCTCGCGACCCTAACGCTCCTCGCCTCCCTGACTCCACATCGTCGCTGGTCGGACCTCGATTGAGTCTTCACAACCGTGTCCAATACCTAATTCGCGCCGGTGACCTAGACGCCGCCTCGGCCGCCGCTCGTCACTCGGTGTTCTCATCGACTCGCCCGACGGTTTTCACTTGCAACGCAATCATCGCTGCTATGTATCGGGCTAAGAGGTATAACGAAGCTATTGCACTTTTTCATTTCTTCTTCAACCAATCTAACCTTGTTCCGAATATCGTTTCTTACAACAATGTTATCAATACTCACTGCGATGAAGGCCGTGTCGATGTGGCTCTCGAGGTTTATCGCCACATCATCTCGCATGCTCCTTTTGGTCCTTCATCAGTAACTTATCGTCATCTCACCAAAGGTTTGATCAGTGTTGGTCGCATTGATGAAGCTGTTGATTTGTTGCGTGAGATGTTGGCTAAAAATCATGGTGCTGATTCAATGGTTTATAATAATATGATATCGGGTTTTCTTGAACTTGGTAATTTAGATAAGGCTAATGAGCTTTTTGATGAGCTTAAGAATCGCTGTATTGTTTATGATGGTGTTGTGAATGCTACTTATATGGAATGGTTTTTTAAGCAGGGAAGAGATAAGGAAGCCATGGATTCGTATATGTCTTTGATGGATCGTGGGTTTAGGATGACTCCTGCCACTTGTAATGTTTTGTTGGAGGTTTTGCTTAAGCATGATAAGCAAAAGGAGGCTTCCGAATTGTTTGATCAGATGTTGGATAATCACACTCCTCCAAATTTTCAAGCTGTTAATTCCGATACTTTTAATATCATGGTTAATGAGTGCTTTAAGCATGGTAAGTTTGATGAAGCCCTTGATACTTTTAGGAAGGTTGGTACCAAGATAAATTCTAAGCCTTTTATAATGGATGTTGCCGGGTATCGTAATATTATCTCTAGGTTTTGTGAGATTGGGATGATGTCGGAAGCTGACACATTGTTTGAAGAGTACTGCTCCAAGCATTTGAGTGCGGATGTGCCTACTCACACTGCTATGATAGATGGTTATTTGAAAGTGGGTAACATTGATGATGCTTTGAGGATATTTAACAAGATGGTGGATTGTGGTTTGAGGGTGACTGCTACCTTTGGCAATAGGGTTTTTGATGAATTGATTAAGAATGGCAAAGTTAATGAGTGTGCTCGTATTTTGAGCAAAATGGGAGGACAAGATCCCAAGCCTGATTCCACTTGCTATGAGGTTGTGATCAAGGGACTGTGTGCTGAAGGTTTGTTGGATAAGAGTCAAGAGTTGTTGGACGAGGTTATGAGGTATGGTGTAGGGGTCACTCTTGCCTTGCGTGAATATGTGACTGAGGTTTTCAAAAATGCTGGGAAAGGTGAGGAGATTGAGAGGCTGCTAGATACGAACCGAATTGGATACAATTCCCGTCCAAGACCTGCATACCGTCCACCACCGGCAAGGTCCCCTTCTCAATTGGATAGAACACATAACCCAACTTATGGGCATCCGCAACAGCGCCCACCACTGCCTTCTGCTCAGATGGCTGGAGTGCACAACCCACCTTCTGAGTTTCCATCTCGTATTGTAGCACCTCAGCAATACCGATACCAAACACGGCCTTCTCAAGTTGTAGGAACGCATAACCAACCTTCTGGGTTTCCAGCTCAAGTAGCAGCACAAAATTCCCCACCACACTTTGATACTCGAATGGGTGGAATACACAACCCGCCTTATGAGTTTCCTTCTCAAATGGCAGCACATCAACGATACCAAACACCGCCTTCTCGGGTGCCAGGAGCACATAACGAACCTTCTAGATTTCCGGTTCAAATGGCAGCACAAAATTTCCCACCGCACTTTGATACTCGAATGGCTGGAGCAGATAACAACCGCTTTCCCCCTAGATATCCACCTCCAATCTCAGGGCAACAACACCCACTGTCTCCTCAAATGACAGCAAATGGACCTTCTCACTCAACATGA |
| Protein sequence |
>MsG0880046782.01.T01 MSLCRLLLRSLRRPTTVSPISITTRSFAFSSAEEAAAERRRRKRRLRIEPPLNAIRPPPPQHQPRDPNAPRLPDSTSSLVGPRLSLHNRVQYLIRAGDLDAASAAARHSVFSSTRPTVFTCNAIIAAMYRAKRYNEAIALFHFFFNQSNLVPNIVSYNNVINTHCDEGRVDVALEVYRHIISHAPFGPSSVTYRHLTKGLISVGRIDEAVDLLREMLAKNHGADSMVYNNMISGFLELGNLDKANELFDELKNRCIVYDGVVNATYMEWFFKQGRDKEAMDSYMSLMDRGFRMTPATCNVLLEVLLKHDKQKEASELFDQMLDNHTPPNFQAVNSDTFNIMVNECFKHGKFDEALDTFRKVGTKINSKPFIMDVAGYRNIISRFCEIGMMSEADTLFEEYCSKHLSADVPTHTAMIDGYLKVGNIDDALRIFNKMVDCGLRVTATFGNRVFDELIKNGKVNECARILSKMGGQDPKPDSTCYEVVIKGLCAEGLLDKSQELLDEVMRYGVGVTLALREYVTEVFKNAGKGEEIERLLDTNRIGYNSRPRPAYRPPPARSPSQLDRTHNPTYGHPQQRPPLPSAQMAGVHNPPSEFPSRIVAPQQYRYQTRPSQVVGTHNQPSGFPAQVAAQNSPPHFDTRMGGIHNPPYEFPSQMAAHQRYQTPPSRVPGAHNEPSRFPVQMAAQNFPPHFDTRMAGADNNRFPPRYPPPISGQQHPLSPQMTANGPSHST* |
